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Fusion Protein:CYP17A1-INTS8 |
Fusion Gene and Fusion Protein Summary |
 Fusion gene summary |
| Fusion partner gene information | Fusion gene name: CYP17A1-INTS8 | FusionPDB ID: 21000 | FusionGDB2.0 ID: 21000 | Hgene | Tgene | Gene symbol | CYP17A1 | INTS8 | Gene ID | 1586 | 55656 |
| Gene name | cytochrome P450 family 17 subfamily A member 1 | integrator complex subunit 8 | |
| Synonyms | CPT7|CYP17|P450C17|S17AH | C8orf52|INT8|NEDCHS | |
| Cytomap | 10q24.32 | 8q22.1 | |
| Type of gene | protein-coding | protein-coding | |
| Description | steroid 17-alpha-hydroxylase/17,20 lyase17-alpha-hydroxyprogesterone aldolaseCYPXVIIcytochrome P450 17A1cytochrome P450, family 17, subfamily A, polypeptide 1cytochrome P450, subfamily XVII (steroid 17-alpha-hydroxylase), adrenal hyperplasiacytochro | integrator complex subunit 8protein kaonashi-1 | |
| Modification date | 20200313 | 20200313 | |
| UniProtAcc | P05093 Main function of 5'-partner protein: FUNCTION: A cytochrome P450 monooxygenase involved in corticoid and androgen biosynthesis (PubMed:9452426, PubMed:27339894, PubMed:22266943, PubMed:25301938). Catalyzes 17-alpha hydroxylation of C21 steroids, which is common for both pathways. A second oxidative step, required only for androgen synthesis, involves an acyl-carbon cleavage. The 17-alpha hydroxy intermediates, as part of adrenal glucocorticoids biosynthesis pathway, are precursors of cortisol (PubMed:9452426, PubMed:25301938) (Probable). Hydroxylates steroid hormones, pregnenolone and progesterone to form 17-alpha hydroxy metabolites, followed by the cleavage of the C17-C20 bond to form C19 steroids, dehydroepiandrosterone (DHEA) and androstenedione (PubMed:9452426, PubMed:27339894, PubMed:22266943, PubMed:25301938). Has 16-alpha hydroxylase activity. Catalyzes 16-alpha hydroxylation of 17-alpha hydroxy pregnenolone, followed by the cleavage of the C17-C20 bond to form 16-alpha-hydroxy DHEA. Also 16-alpha hydroxylates androgens, relevant for estriol synthesis (PubMed:27339894, PubMed:25301938). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed:9452426, PubMed:27339894, PubMed:22266943, PubMed:25301938). {ECO:0000269|PubMed:22266943, ECO:0000269|PubMed:25301938, ECO:0000269|PubMed:27339894, ECO:0000269|PubMed:9452426, ECO:0000305|PubMed:8027220}. | Q75QN2 Main function of 5'-partner protein: FUNCTION: Component of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. {ECO:0000269|PubMed:28542170}. | |
| Ensembl transtripts involved in fusion gene | ENST ids | ENST00000369887, ENST00000489268, | ENST00000520845, ENST00000447247, ENST00000523731, |
| Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0) | * DoF score | 7 X 6 X 3=126 | 5 X 5 X 5=125 |
| # samples | 7 | 5 | |
| ** MAII score | log2(7/126*10)=-0.84799690655495 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | log2(5/125*10)=-1.32192809488736 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | |
| Fusion gene context | PubMed: CYP17A1 [Title/Abstract] AND INTS8 [Title/Abstract] AND fusion [Title/Abstract] | ||
| Fusion neoantigen context | PubMed: CYP17A1 [Title/Abstract] AND INTS8 [Title/Abstract] AND neoantigen [Title/Abstract] | ||
| Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0) | CYP17A1(104596822)-INTS8(95861690), # samples:1 | ||
| Anticipated loss of major functional domain due to fusion event. | |||
| * DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
| Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
| Partner | Gene | GO ID | GO term | PubMed ID |
| Hgene | CYP17A1 | GO:0008202 | steroid metabolic process | 22266943 |
| Hgene | CYP17A1 | GO:0042446 | hormone biosynthetic process | 22266943 |
| Hgene | CYP17A1 | GO:0042448 | progesterone metabolic process | 22266943 |
| Tgene | INTS8 | GO:0016180 | snRNA processing | 16239144 |
| Four levels of functional features of fusion genes Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr10:104596822/chr8:95861690) - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. - How to search 1. Put your fusion gene symbol. 2. Press the tab key until there will be shown the breakpoint information filled. 4. Go down and press 'Search' tab twice. 4. Go down to have the hyperlink of the search result. 5. Click the hyperlink. 6. See the FGviewer result for your fusion gene. |
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| Retention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here. |
| Fusion gene breakpoints across CYP17A1 (5'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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| Fusion gene breakpoints across INTS8 (3'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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Fusion Amino Acid Sequences |
| Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
| ENST00000369887 | CYP17A1 | chr10 | 104596822 | - | ENST00000523731 | INTS8 | chr8 | 95861690 | + | 3709 | 469 | 115 | 2196 | 693 |
| ENST00000369887 | CYP17A1 | chr10 | 104596822 | - | ENST00000447247 | INTS8 | chr8 | 95861690 | + | 2404 | 469 | 115 | 2145 | 676 |
| DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
| ENST00000369887 | ENST00000523731 | CYP17A1 | chr10 | 104596822 | - | INTS8 | chr8 | 95861690 | + | 0.000227231 | 0.9997727 |
| ENST00000369887 | ENST00000447247 | CYP17A1 | chr10 | 104596822 | - | INTS8 | chr8 | 95861690 | + | 0.000459861 | 0.9995402 |
| Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones. |
Get the fusion protein sequences from here. |
| Fusion protein sequence information is available in the fasta format. >FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP |
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Fusion Protein Breakpoint Sequences for CYP17A1-INTS8 |
| +/-13 AA sequence from the breakpoints of the fusion protein sequences. |
| Hgene | Hchr | Hbp | Tgene | Tchr | Tbp | Length(fusion protein) | BP in fusion protein | Peptide |
| CYP17A1 | chr10 | 104596822 | INTS8 | chr8 | 95861690 | 469 | 118 | IKKGKDFSGRPQMVCSRSVNLEKASE |
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Potential FusionNeoAntigen Information of CYP17A1-INTS8 in HLA I |
| Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific. |
| CYP17A1-INTS8_104596822_95861690.msa |
| Potential FusionNeoAntigen Information * We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5) |
| Fusion gene | Hchr | Hbp | Tgene | Tchr | Tbp | HLA I | FusionNeoAntigen peptide | Binding score | Immunogenic score | Neoantigen start (at BP 13) | Neoantigen end (at BP 13) |
| CYP17A1-INTS8 | chr10 | 104596822 | chr8 | 95861690 | 469 | HLA-B42:01 | RPQMVCSRSV | 0.8239 | 0.7151 | 9 | 19 |
| CYP17A1-INTS8 | chr10 | 104596822 | chr8 | 95861690 | 469 | HLA-A30:01 | MVCSRSVNLEK | 0.9906 | 0.9059 | 12 | 23 |
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Potential FusionNeoAntigen Information of CYP17A1-INTS8 in HLA II |
| Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific. |
| Potential FusionNeoAntigen Information * We used NetMHCIIpan v4.1 (%rank<0.5). |
| Fusion gene | Hchr | Hbp | Tgene | Tchr | Tbp | HLA II | FusionNeoAntigen peptide | Neoantigen start (at BP 13) | Neoantigen end (at BP 13) |
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Fusion breakpoint peptide structures of CYP17A1-INTS8 |
| 3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens * The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA. |
| File name | BPseq | Hgene | Tgene | Hchr | Hbp | Tchr | Tbp | AAlen |
| 2566 | FSGRPQMVCSRSVN | CYP17A1 | INTS8 | chr10 | 104596822 | chr8 | 95861690 | 469 |
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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of CYP17A1-INTS8 |
| Virtual screening between 25 HLAs (from PDB) and FusionNeoAntigens * We used Glide to predict the interaction between HLAs and neoantigens. |
| HLA allele | PDB ID | File name | BPseq | Docking score | Glide score |
| HLA-B14:02 | 3BVN | 2566 | FSGRPQMVCSRSVN | -7.9962 | -8.1096 |
| HLA-B14:02 | 3BVN | 2566 | FSGRPQMVCSRSVN | -5.70842 | -6.74372 |
| HLA-B52:01 | 3W39 | 2566 | FSGRPQMVCSRSVN | -6.83737 | -6.95077 |
| HLA-B52:01 | 3W39 | 2566 | FSGRPQMVCSRSVN | -4.4836 | -5.5189 |
| HLA-A11:01 | 4UQ2 | 2566 | FSGRPQMVCSRSVN | -10.0067 | -10.1201 |
| HLA-A11:01 | 4UQ2 | 2566 | FSGRPQMVCSRSVN | -9.03915 | -10.0745 |
| HLA-A24:02 | 5HGA | 2566 | FSGRPQMVCSRSVN | -6.56204 | -6.67544 |
| HLA-A24:02 | 5HGA | 2566 | FSGRPQMVCSRSVN | -5.42271 | -6.45801 |
| HLA-B44:05 | 3DX8 | 2566 | FSGRPQMVCSRSVN | -7.85648 | -8.89178 |
| HLA-B44:05 | 3DX8 | 2566 | FSGRPQMVCSRSVN | -5.3978 | -5.5112 |
| HLA-A02:01 | 6TDR | 2566 | FSGRPQMVCSRSVN | -3.37154 | -4.40684 |
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Vaccine Design for the FusionNeoAntigens of CYP17A1-INTS8 |
| mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is. |
| Fusion gene | Hchr | Hbp | Tchr | Tbp | Start in +/-13AA | End in +/-13AA | FusionNeoAntigen peptide sequence | FusionNeoAntigen RNA sequence |
| CYP17A1-INTS8 | chr10 | 104596822 | chr8 | 95861690 | 12 | 23 | MVCSRSVNLEK | ATGGTATGTTCAAGATCAGTAAATTTAGAAAAA |
| CYP17A1-INTS8 | chr10 | 104596822 | chr8 | 95861690 | 9 | 19 | RPQMVCSRSV | CGGCCTCAAATGGTATGTTCAAGATCAGTA |
| mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs. |
| Fusion gene | Hchr | Hbp | Tchr | Tbp | Start in +/-13AA | End in +/-13AA | FusionNeoAntigen peptide | FusionNEoAntigen RNA sequence |
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Information of the samples that have these potential fusion neoantigens of CYP17A1-INTS8 |
| These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens. |
| Cancer type | Fusion gene | Hchr | Hbp | Henst | Tchr | Tbp | Tenst | Sample |
| Non-Cancer | CYP17A1-INTS8 | chr10 | 104596822 | ENST00000369887 | chr8 | 95861690 | ENST00000447247 | TCGA-P8-A5KD-11A |
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Potential target of CAR-T therapy development for CYP17A1-INTS8 |
| Predicted 3D structure. We used RoseTTAFold. |
| Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features * Minus value of BPloci means that the break point is located before the CDS. |
| - In-frame and retained 'Transmembrane'. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| Subcellular localization prediction of the transmembrane domain retained fusion proteins * We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image. |
| Hgene | Hchr | Hbp | Henst | Tgene | Tchr | Tbp | Tenst | DeepLoc result |
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Related Drugs to CYP17A1-INTS8 |
| Drugs used for this fusion-positive patient. (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
| Hgene | Tgene | Drug | Source | PMID |
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Related Diseases to CYP17A1-INTS8 |
| Diseases that have this fusion gene. (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
| Hgene | Tgene | Disease | Source | PMID |
| Diseases associated with fusion partners. (DisGeNet 4.0) |
| Partner | Gene | Disease ID | Disease name | # pubmeds | Source |
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