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Center for Computational Systems Medicine
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Fusion Gene and Fusion Protein Summary

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Fusion Amino Acid Sequences (multiple BPs and multiple gene isoforms)

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Fusion Protein Breakpoint Sequences - (for the Screening of the FusionNeoAntigens)

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Potential FusionNeoAntigens in HLA I - (netMHCpan v4.1 + deepHLApan v1.1)

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Potential FusionNeoAntigens in HLA II - (netMHCIIpan v4.1)

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Fusion Breakpoint 14 AA Peptide Structure - (RoseTTAFold)

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D - (Glide)

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Vaccine Design for the FusionNeoAntigens (RNA/protein sequences)

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Potential target of CAR-T therapy development

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Information on the samples that have these potential fusion neoantigens

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Fusion Protein Targeting Drugs - (Manual Curation)

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Fusion Protein Related diseases - (Manual Curation)

Fusion Protein:ENDOV-IKZF3

Fusion Gene and Fusion Protein Summary

check button Fusion gene summary
Fusion partner gene informationFusion gene name: ENDOV-IKZF3
FusionPDB ID: 26558
FusionGDB2.0 ID: 26558
HgeneTgene
Gene symbol

ENDOV

IKZF3

Gene ID

284131

22806

Gene nameendonuclease VIKAROS family zinc finger 3
Synonyms-AIO|AIOLOS|ZNFN1A3
Cytomap

17q25.3

17q12-q21.1

Type of geneprotein-codingprotein-coding
Descriptionendonuclease VhEndoVinosine-specific endoribonucleasezinc finger protein Aioloszinc finger DNA binding protein Aioloszinc finger protein, subfamily 1A, 3 (Aiolos)
Modification date2020031320200327
UniProtAcc

Q8N8Q3

Main function of 5'-partner protein: FUNCTION: [Isoform 1]: Endoribonuclease that specifically cleaves inosine-containing RNAs: cleaves RNA at the second phosphodiester bond 3' to inosine (PubMed:23912683, PubMed:23912718, PubMed:27573237, PubMed:31703097, PubMed:25195743). Active against both single-stranded and double-stranded RNAs (PubMed:31703097, PubMed:25195743). Has strong preference for single-stranded RNAs (ssRNAs) toward double-stranded RNAs (dsRNAs) (PubMed:23912718). Cleaves mRNAs and tRNAs containing inosine (PubMed:23912683, PubMed:31703097). Also able to cleave structure-specific dsRNA substrates containing the specific sites 5'-IIUI-3' and 5'-UIUU-3' (PubMed:23912718, PubMed:27573237). Inosine is present in a number of RNAs following editing; the function of inosine-specific endoribonuclease is still unclear: it could either play a regulatory role in edited RNAs, or be involved in antiviral response by removing the hyperedited long viral dsRNA genome that has undergone A-to-I editing (Probable). Binds branched DNA structures (PubMed:23139746). {ECO:0000269|PubMed:23139746, ECO:0000269|PubMed:23912683, ECO:0000269|PubMed:23912718, ECO:0000269|PubMed:25195743, ECO:0000269|PubMed:27573237, ECO:0000269|PubMed:31703097, ECO:0000305}.; FUNCTION: [Isoform 6]: Endoribonuclease that specifically cleaves inosine-containing RNAs: cleaves RNA at the second phosphodiester bond 3' to inosine (PubMed:31703097). Active against both single-stranded and double-stranded RNAs (PubMed:31703097). Cleaves tRNAs containing inosine (PubMed:31703097). {ECO:0000269|PubMed:31703097}.; FUNCTION: [Isoform 7]: Endoribonuclease that specifically cleaves inosine-containing RNAs: cleaves RNA at the second phosphodiester bond 3' to inosine (PubMed:31703097). Active against both single-stranded and double-stranded RNAs (PubMed:31703097). Cleaves tRNAs containing inosine (PubMed:31703097). {ECO:0000269|PubMed:31703097}.

Q9UKT9

Main function of 5'-partner protein: FUNCTION: Transcription factor that plays an important role in the regulation of lymphocyte differentiation. Plays an essential role in regulation of B-cell differentiation, proliferation and maturation to an effector state. Involved in regulating BCL2 expression and controlling apoptosis in T-cells in an IL2-dependent manner. {ECO:0000269|PubMed:10369681}.
Ensembl transtripts involved in fusion geneENST idsENST00000323854, ENST00000517295, 
ENST00000517795, ENST00000518137, 
ENST00000518901, ENST00000520367, 
ENST00000522751, ENST00000518644, 
ENST00000518907, ENST00000520284, 
ENST00000521847, ENST00000523999, 
ENST00000346872, ENST00000377944, 
ENST00000377945, ENST00000377952, 
ENST00000377958, ENST00000394189, 
ENST00000439016, ENST00000439167, 
ENST00000535189, ENST00000583368, 
ENST00000346243, ENST00000350532, 
ENST00000351680, ENST00000467757, 
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)* DoF score4 X 4 X 3=4834 X 12 X 14=5712
# samples 449
** MAII scorelog2(4/48*10)=-0.263034405833794
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(49/5712*10)=-3.54314232502653
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Fusion gene context

PubMed: ENDOV [Title/Abstract] AND IKZF3 [Title/Abstract] AND fusion [Title/Abstract]

Fusion neoantigen context

PubMed: ENDOV [Title/Abstract] AND IKZF3 [Title/Abstract] AND neoantigen [Title/Abstract]

Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)ENDOV(78403631)-IKZF3(37922746), # samples:2
Anticipated loss of major functional domain due to fusion event.ENDOV-IKZF3 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
ENDOV-IKZF3 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
ENDOV-IKZF3 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
ENDOV-IKZF3 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
ENDOV-IKZF3 seems lost the major protein functional domain in Tgene partner, which is a epigenetic factor due to the frame-shifted ORF.
ENDOV-IKZF3 seems lost the major protein functional domain in Tgene partner, which is a essential gene due to the frame-shifted ORF.
ENDOV-IKZF3 seems lost the major protein functional domain in Tgene partner, which is a transcription factor due to the frame-shifted ORF.
ENDOV-IKZF3 seems lost the major protein functional domain in Tgene partner, which is a tumor suppressor due to the frame-shifted ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr17:78403631/chr17:37922746)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonRetention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here.

check buttonFusion gene breakpoints across ENDOV (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check buttonFusion gene breakpoints across IKZF3 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure


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Fusion Amino Acid Sequences


check buttonFusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000520367ENDOVchr1778403631+ENST00000346872IKZF3chr1737922746-2280731281434468
ENST00000520367ENDOVchr1778403631+ENST00000394189IKZF3chr1737922746-1941731281434468
ENST00000520367ENDOVchr1778403631+ENST00000439167IKZF3chr1737922746-1941731281434468
ENST00000520367ENDOVchr1778403631+ENST00000535189IKZF3chr1737922746-1941731281434468
ENST00000520367ENDOVchr1778403631+ENST00000377944IKZF3chr1737922746-1941731281434468
ENST00000520367ENDOVchr1778403631+ENST00000377945IKZF3chr1737922746-1941731281434468
ENST00000520367ENDOVchr1778403631+ENST00000377952IKZF3chr1737922746-1941731281434468
ENST00000520367ENDOVchr1778403631+ENST00000377958IKZF3chr1737922746-1941731281434468
ENST00000518137ENDOVchr1778403631+ENST00000346872IKZF3chr1737922746-2415866281569513
ENST00000518137ENDOVchr1778403631+ENST00000394189IKZF3chr1737922746-2076866281569513
ENST00000518137ENDOVchr1778403631+ENST00000439167IKZF3chr1737922746-2076866281569513
ENST00000518137ENDOVchr1778403631+ENST00000535189IKZF3chr1737922746-2076866281569513
ENST00000518137ENDOVchr1778403631+ENST00000377944IKZF3chr1737922746-2076866281569513
ENST00000518137ENDOVchr1778403631+ENST00000377945IKZF3chr1737922746-2076866281569513
ENST00000518137ENDOVchr1778403631+ENST00000377952IKZF3chr1737922746-2076866281569513
ENST00000518137ENDOVchr1778403631+ENST00000377958IKZF3chr1737922746-2076866281569513
ENST00000323854ENDOVchr1778403631+ENST00000346243IKZF3chr1737922746-1871116710521870273
ENST00000323854ENDOVchr1778403631+ENST00000350532IKZF3chr1737922746-1871116710521870273
ENST00000323854ENDOVchr1778403631+ENST00000351680IKZF3chr1737922746-1871116710521870273
ENST00000323854ENDOVchr1778403631+ENST00000467757IKZF3chr1737922746-1871116710521870273
ENST00000522751ENDOVchr1778403631+ENST00000439016IKZF3chr1737922746-2120141613102119270
ENST00000522751ENDOVchr1778403631+ENST00000583368IKZF3chr1737922746-2414141613102119269
ENST00000517795ENDOVchr1778403631+ENST00000346872IKZF3chr1737922746-21435941971297366
ENST00000517795ENDOVchr1778403631+ENST00000394189IKZF3chr1737922746-18045941971297366
ENST00000517795ENDOVchr1778403631+ENST00000439167IKZF3chr1737922746-18045941971297366
ENST00000517795ENDOVchr1778403631+ENST00000535189IKZF3chr1737922746-18045941971297366
ENST00000517795ENDOVchr1778403631+ENST00000377944IKZF3chr1737922746-18045941971297366
ENST00000517795ENDOVchr1778403631+ENST00000377945IKZF3chr1737922746-18045941971297366
ENST00000517795ENDOVchr1778403631+ENST00000377952IKZF3chr1737922746-18045941971297366
ENST00000517795ENDOVchr1778403631+ENST00000377958IKZF3chr1737922746-18045941971297366
ENST00000518901ENDOVchr1778403631+ENST00000346872IKZF3chr1737922746-2148599821302406
ENST00000518901ENDOVchr1778403631+ENST00000394189IKZF3chr1737922746-1809599821302406
ENST00000518901ENDOVchr1778403631+ENST00000439167IKZF3chr1737922746-1809599821302406
ENST00000518901ENDOVchr1778403631+ENST00000535189IKZF3chr1737922746-1809599821302406
ENST00000518901ENDOVchr1778403631+ENST00000377944IKZF3chr1737922746-1809599821302406
ENST00000518901ENDOVchr1778403631+ENST00000377945IKZF3chr1737922746-1809599821302406
ENST00000518901ENDOVchr1778403631+ENST00000377952IKZF3chr1737922746-1809599821302406
ENST00000518901ENDOVchr1778403631+ENST00000377958IKZF3chr1737922746-1809599821302406
ENST00000517295ENDOVchr1778403631+ENST00000346872IKZF3chr1737922746-2345796571499480
ENST00000517295ENDOVchr1778403631+ENST00000394189IKZF3chr1737922746-2006796571499480
ENST00000517295ENDOVchr1778403631+ENST00000439167IKZF3chr1737922746-2006796571499480
ENST00000517295ENDOVchr1778403631+ENST00000535189IKZF3chr1737922746-2006796571499480
ENST00000517295ENDOVchr1778403631+ENST00000377944IKZF3chr1737922746-2006796571499480
ENST00000517295ENDOVchr1778403631+ENST00000377945IKZF3chr1737922746-2006796571499480
ENST00000517295ENDOVchr1778403631+ENST00000377952IKZF3chr1737922746-2006796571499480
ENST00000517295ENDOVchr1778403631+ENST00000377958IKZF3chr1737922746-2006796571499480

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000520367ENST00000346872ENDOVchr1778403631+IKZF3chr1737922746-0.00477360.9952264
ENST00000520367ENST00000394189ENDOVchr1778403631+IKZF3chr1737922746-0.0060741530.99392587
ENST00000520367ENST00000439167ENDOVchr1778403631+IKZF3chr1737922746-0.0060741530.99392587
ENST00000520367ENST00000535189ENDOVchr1778403631+IKZF3chr1737922746-0.0060741530.99392587
ENST00000520367ENST00000377944ENDOVchr1778403631+IKZF3chr1737922746-0.0060741530.99392587
ENST00000520367ENST00000377945ENDOVchr1778403631+IKZF3chr1737922746-0.0060741530.99392587
ENST00000520367ENST00000377952ENDOVchr1778403631+IKZF3chr1737922746-0.0060741530.99392587
ENST00000520367ENST00000377958ENDOVchr1778403631+IKZF3chr1737922746-0.0060741530.99392587
ENST00000518137ENST00000346872ENDOVchr1778403631+IKZF3chr1737922746-0.0076685520.9923314
ENST00000518137ENST00000394189ENDOVchr1778403631+IKZF3chr1737922746-0.0096948350.9903052
ENST00000518137ENST00000439167ENDOVchr1778403631+IKZF3chr1737922746-0.0096948350.9903052
ENST00000518137ENST00000535189ENDOVchr1778403631+IKZF3chr1737922746-0.0096948350.9903052
ENST00000518137ENST00000377944ENDOVchr1778403631+IKZF3chr1737922746-0.0096948350.9903052
ENST00000518137ENST00000377945ENDOVchr1778403631+IKZF3chr1737922746-0.0096948350.9903052
ENST00000518137ENST00000377952ENDOVchr1778403631+IKZF3chr1737922746-0.0096948350.9903052
ENST00000518137ENST00000377958ENDOVchr1778403631+IKZF3chr1737922746-0.0096948350.9903052
ENST00000323854ENST00000346243ENDOVchr1778403631+IKZF3chr1737922746-0.0033902570.99660975
ENST00000323854ENST00000350532ENDOVchr1778403631+IKZF3chr1737922746-0.0033902570.99660975
ENST00000323854ENST00000351680ENDOVchr1778403631+IKZF3chr1737922746-0.0033902570.99660975
ENST00000323854ENST00000467757ENDOVchr1778403631+IKZF3chr1737922746-0.0033902570.99660975
ENST00000522751ENST00000439016ENDOVchr1778403631+IKZF3chr1737922746-0.0100842690.9899157
ENST00000522751ENST00000583368ENDOVchr1778403631+IKZF3chr1737922746-0.00905410.99094594
ENST00000517795ENST00000346872ENDOVchr1778403631+IKZF3chr1737922746-0.0045967780.9954032
ENST00000517795ENST00000394189ENDOVchr1778403631+IKZF3chr1737922746-0.0057855020.9942145
ENST00000517795ENST00000439167ENDOVchr1778403631+IKZF3chr1737922746-0.0057855020.9942145
ENST00000517795ENST00000535189ENDOVchr1778403631+IKZF3chr1737922746-0.0057855020.9942145
ENST00000517795ENST00000377944ENDOVchr1778403631+IKZF3chr1737922746-0.0057855020.9942145
ENST00000517795ENST00000377945ENDOVchr1778403631+IKZF3chr1737922746-0.0057855020.9942145
ENST00000517795ENST00000377952ENDOVchr1778403631+IKZF3chr1737922746-0.0057855020.9942145
ENST00000517795ENST00000377958ENDOVchr1778403631+IKZF3chr1737922746-0.0057855020.9942145
ENST00000518901ENST00000346872ENDOVchr1778403631+IKZF3chr1737922746-0.0042821750.9957178
ENST00000518901ENST00000394189ENDOVchr1778403631+IKZF3chr1737922746-0.0052006990.99479926
ENST00000518901ENST00000439167ENDOVchr1778403631+IKZF3chr1737922746-0.0052006990.99479926
ENST00000518901ENST00000535189ENDOVchr1778403631+IKZF3chr1737922746-0.0052006990.99479926
ENST00000518901ENST00000377944ENDOVchr1778403631+IKZF3chr1737922746-0.0052006990.99479926
ENST00000518901ENST00000377945ENDOVchr1778403631+IKZF3chr1737922746-0.0052006990.99479926
ENST00000518901ENST00000377952ENDOVchr1778403631+IKZF3chr1737922746-0.0052006990.99479926
ENST00000518901ENST00000377958ENDOVchr1778403631+IKZF3chr1737922746-0.0052006990.99479926
ENST00000517295ENST00000346872ENDOVchr1778403631+IKZF3chr1737922746-0.0098185990.9901814
ENST00000517295ENST00000394189ENDOVchr1778403631+IKZF3chr1737922746-0.0128355670.9871644
ENST00000517295ENST00000439167ENDOVchr1778403631+IKZF3chr1737922746-0.0128355670.9871644
ENST00000517295ENST00000535189ENDOVchr1778403631+IKZF3chr1737922746-0.0128355670.9871644
ENST00000517295ENST00000377944ENDOVchr1778403631+IKZF3chr1737922746-0.0128355670.9871644
ENST00000517295ENST00000377945ENDOVchr1778403631+IKZF3chr1737922746-0.0128355670.9871644
ENST00000517295ENST00000377952ENDOVchr1778403631+IKZF3chr1737922746-0.0128355670.9871644
ENST00000517295ENST00000377958ENDOVchr1778403631+IKZF3chr1737922746-0.0128355670.9871644

check button Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones.

Get the fusion protein sequences from here.

Fusion protein sequence information is available in the fasta format.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

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Fusion Protein Breakpoint Sequences for ENDOV-IKZF3

check button +/-13 AA sequence from the breakpoints of the fusion protein sequences.
HgeneHchrHbpTgeneTchrTbpLength(fusion protein)BP in fusion proteinPeptide
ENDOVchr1778403631IKZF3chr1737922746116739PLSIDGTCLLFFSEKRHCFDVNYNSS
ENDOVchr1778403631IKZF3chr1737922746141636AACALPTPFNRWSEKRHCFDVNYNSS
ENDOVchr1778403631IKZF3chr1737922746594132PVACPKGDSGESSGEKRHCFDVNYNS
ENDOVchr1778403631IKZF3chr1737922746599172PVACPKGDSGESSGEKRHCFDVNYNS
ENDOVchr1778403631IKZF3chr1737922746731234PVACPKGDSGESSGEKRHCFDVNYNS
ENDOVchr1778403631IKZF3chr1737922746796246PVACPKGDSGESSGEKRHCFDVNYNS
ENDOVchr1778403631IKZF3chr1737922746866279PVACPKGDSGESSGEKRHCFDVNYNS

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Potential FusionNeoAntigen Information of ENDOV-IKZF3 in HLA I

check button Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.
ENDOV-IKZF3_78403631_37922746.msa

check button Potential FusionNeoAntigen Information
* We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5)
Fusion geneHchrHbpTgeneTchrTbpHLA IFusionNeoAntigen peptideBinding scoreImmunogenic scoreNeoantigen start (at BP 13)Neoantigen end (at BP 13)
ENDOV-IKZF3chr1778403631chr17379227461416HLA-A24:25RWSEKRHCF0.95240.56051019
ENDOV-IKZF3chr1778403631chr17379227461416HLA-A24:20RWSEKRHCF0.94170.55751019
ENDOV-IKZF3chr1778403631chr17379227461416HLA-A24:15RWSEKRHCF0.93990.55121019
ENDOV-IKZF3chr1778403631chr17379227461416HLA-A24:31RWSEKRHCF0.89860.56611019
ENDOV-IKZF3chr1778403631chr17379227461416HLA-A24:17RWSEKRHCF0.83430.64771019
ENDOV-IKZF3chr1778403631chr17379227461416HLA-A31:08RWSEKRHCF0.78150.5311019
ENDOV-IKZF3chr1778403631chr17379227461416HLA-A24:14RWSEKRHCF0.62150.65661019
ENDOV-IKZF3chr1778403631chr1737922746796HLA-B44:03GESSGEKRHCF0.99820.9142920
ENDOV-IKZF3chr1778403631chr17379227461416HLA-A24:02RWSEKRHCF0.94170.55751019
ENDOV-IKZF3chr1778403631chr17379227461416HLA-B73:01NRWSEKRHC0.79630.6187918
ENDOV-IKZF3chr1778403631chr17379227461167HLA-C07:05FFSEKRHCF0.78170.94121019
ENDOV-IKZF3chr1778403631chr17379227461167HLA-B15:21FFSEKRHCF0.75230.79911019
ENDOV-IKZF3chr1778403631chr17379227461167HLA-C12:16FFSEKRHCF0.73820.95191019
ENDOV-IKZF3chr1778403631chr17379227461167HLA-C07:19FFSEKRHCF0.65680.61691019
ENDOV-IKZF3chr1778403631chr17379227461167HLA-C07:10FFSEKRHCF0.65220.93951019
ENDOV-IKZF3chr1778403631chr17379227461167HLA-C07:67FFSEKRHCF0.63840.90391019
ENDOV-IKZF3chr1778403631chr17379227461167HLA-C07:80FFSEKRHCF0.63840.90391019
ENDOV-IKZF3chr1778403631chr17379227461167HLA-C07:95FFSEKRHCF0.61610.5621019
ENDOV-IKZF3chr1778403631chr17379227461167HLA-C07:46FFSEKRHCF0.61130.81091019
ENDOV-IKZF3chr1778403631chr17379227461167HLA-C07:27FFSEKRHCF0.55730.92271019
ENDOV-IKZF3chr1778403631chr17379227461167HLA-C07:29FFSEKRHCF0.53240.90581019
ENDOV-IKZF3chr1778403631chr17379227461167HLA-C07:13FFSEKRHCF0.4870.84651019
ENDOV-IKZF3chr1778403631chr17379227461167HLA-C04:07FFSEKRHCF0.22710.62231019
ENDOV-IKZF3chr1778403631chr17379227461167HLA-C04:10FFSEKRHCF0.17970.60541019
ENDOV-IKZF3chr1778403631chr17379227461167HLA-C04:14FFSEKRHCF0.12850.61411019
ENDOV-IKZF3chr1778403631chr17379227461167HLA-C16:01FSEKRHCF0.99040.97091119
ENDOV-IKZF3chr1778403631chr17379227461167HLA-C16:02FSEKRHCF0.98510.97871119
ENDOV-IKZF3chr1778403631chr17379227461167HLA-C07:22FFSEKRHCF0.70790.71571019
ENDOV-IKZF3chr1778403631chr17379227461167HLA-C03:67FFSEKRHCF0.69320.9621019
ENDOV-IKZF3chr1778403631chr17379227461167HLA-C07:17FFSEKRHCF0.64730.94861019
ENDOV-IKZF3chr1778403631chr17379227461167HLA-C07:02FFSEKRHCF0.63840.90391019
ENDOV-IKZF3chr1778403631chr17379227461167HLA-B08:12FFSEKRHCF0.59470.58721019
ENDOV-IKZF3chr1778403631chr17379227461167HLA-C07:01FFSEKRHCF0.58580.55851019
ENDOV-IKZF3chr1778403631chr17379227461167HLA-C07:04FFSEKRHCF0.58120.85141019
ENDOV-IKZF3chr1778403631chr17379227461167HLA-C14:02FFSEKRHCF0.37110.93621019
ENDOV-IKZF3chr1778403631chr17379227461167HLA-C14:03FFSEKRHCF0.37110.93621019
ENDOV-IKZF3chr1778403631chr17379227461167HLA-C04:01FFSEKRHCF0.22710.62231019
ENDOV-IKZF3chr1778403631chr17379227461416HLA-C14:03RWSEKRHCF0.21340.97641019
ENDOV-IKZF3chr1778403631chr17379227461416HLA-C14:02RWSEKRHCF0.21340.97641019
ENDOV-IKZF3chr1778403631chr1737922746796HLA-C16:02SSGEKRHCF0.15260.99111120
ENDOV-IKZF3chr1778403631chr1737922746796HLA-C16:01SSGEKRHCF0.14760.98131120
ENDOV-IKZF3chr1778403631chr17379227461167HLA-C18:01FFSEKRHCF0.1220.63841019
ENDOV-IKZF3chr1778403631chr17379227461167HLA-C06:06FFSEKRHCF0.11250.96521019
ENDOV-IKZF3chr1778403631chr17379227461167HLA-C04:04FFSEKRHCF0.04780.64421019
ENDOV-IKZF3chr1778403631chr1737922746796HLA-B44:26GESSGEKRHCF0.99820.9142920
ENDOV-IKZF3chr1778403631chr1737922746796HLA-B44:13GESSGEKRHCF0.99820.9142920
ENDOV-IKZF3chr1778403631chr1737922746796HLA-B44:07GESSGEKRHCF0.99820.9142920

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Potential FusionNeoAntigen Information of ENDOV-IKZF3 in HLA II

check button Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.

check button Potential FusionNeoAntigen Information
* We used NetMHCIIpan v4.1 (%rank<0.5).
Fusion geneHchrHbpTgeneTchrTbpHLA IIFusionNeoAntigen peptideNeoantigen start (at BP 13)Neoantigen end (at BP 13)

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Fusion breakpoint peptide structures of ENDOV-IKZF3

check button3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens
* The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA.
File nameBPseqHgeneTgeneHchrHbpTchrTbpAAlen
2727GDSGESSGEKRHCFENDOVIKZF3chr1778403631chr1737922746796
File nameBPseqHgeneTgeneHchrHbpTchrTbpAAlen
9268TCLLFFSEKRHCFDENDOVIKZF3chr1778403631chr17379227461167
File nameBPseqHgeneTgeneHchrHbpTchrTbpAAlen
9537TPFNRWSEKRHCFDENDOVIKZF3chr1778403631chr17379227461416

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of ENDOV-IKZF3

check buttonVirtual screening between 25 HLAs (from PDB) and FusionNeoAntigens
* We used Glide to predict the interaction between HLAs and neoantigens.
HLA allelePDB IDFile nameBPseqDocking scoreGlide score
HLA-B51:011E282727GDSGESSGEKRHCF-1.52154-1.53034
HLA-B57:032BVO2727GDSGESSGEKRHCF-1.85408-1.86288
HLA-A03:012XPG2727GDSGESSGEKRHCF-5.74288-5.75168
HLA-B14:023BVN2727GDSGESSGEKRHCF-5.54895-5.55775
HLA-B27:093CZF2727GDSGESSGEKRHCF1000010000
HLA-B52:013W392727GDSGESSGEKRHCF-4.51675-4.52555
HLA-B18:014JQV2727GDSGESSGEKRHCF-0.51997-0.52877
HLA-A11:014UQ22727GDSGESSGEKRHCF-8.63875-8.64755
HLA-A24:025HGA2727GDSGESSGEKRHCF-7.46702-7.47582
HLA-B57:015VUD2727GDSGESSGEKRHCF-3.69897-3.70777
HLA-C08:026JTP2727GDSGESSGEKRHCF-6.02102-6.02982
HLA-B27:056PYJ2727GDSGESSGEKRHCF-6.22207-6.23087
HLA-B27:036PZ52727GDSGESSGEKRHCF-5.51764-5.52644
HLA-B44:053DX82727GDSGESSGEKRHCF-5.57474-5.58354
HLA-B44:021M6O2727GDSGESSGEKRHCF-6.61471-6.62351
HLA-A02:016TDR2727GDSGESSGEKRHCF-3.09316-3.10196
HLA-B14:023BVN9268TCLLFFSEKRHCFD-7.9962-8.1096
HLA-B14:023BVN9268TCLLFFSEKRHCFD-5.70842-6.74372
HLA-B52:013W399268TCLLFFSEKRHCFD-6.83737-6.95077
HLA-B52:013W399268TCLLFFSEKRHCFD-4.4836-5.5189
HLA-A11:014UQ29268TCLLFFSEKRHCFD-10.0067-10.1201
HLA-A11:014UQ29268TCLLFFSEKRHCFD-9.03915-10.0745
HLA-A24:025HGA9268TCLLFFSEKRHCFD-6.56204-6.67544
HLA-A24:025HGA9268TCLLFFSEKRHCFD-5.42271-6.45801
HLA-B44:053DX89268TCLLFFSEKRHCFD-7.85648-8.89178
HLA-B44:053DX89268TCLLFFSEKRHCFD-5.3978-5.5112
HLA-A02:016TDR9268TCLLFFSEKRHCFD-3.37154-4.40684
HLA-B14:023BVN9537TPFNRWSEKRHCFD-7.15543-7.26883
HLA-B14:023BVN9537TPFNRWSEKRHCFD-4.77435-5.80965
HLA-B52:013W399537TPFNRWSEKRHCFD-6.80875-6.92215
HLA-B52:013W399537TPFNRWSEKRHCFD-4.20386-5.23916
HLA-A11:014UQ29537TPFNRWSEKRHCFD-7.5194-8.5547
HLA-A11:014UQ29537TPFNRWSEKRHCFD-6.9601-7.0735
HLA-A24:025HGA9537TPFNRWSEKRHCFD-7.52403-7.63743
HLA-A24:025HGA9537TPFNRWSEKRHCFD-5.82433-6.85963
HLA-B27:056PYJ9537TPFNRWSEKRHCFD-3.28285-4.31815
HLA-B44:053DX89537TPFNRWSEKRHCFD-5.91172-6.94702
HLA-B44:053DX89537TPFNRWSEKRHCFD-4.24346-4.35686

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Vaccine Design for the FusionNeoAntigens of ENDOV-IKZF3

check button mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is.
Fusion geneHchrHbpTchrTbpStart in +/-13AAEnd in +/-13AAFusionNeoAntigen peptide sequenceFusionNeoAntigen RNA sequence
ENDOV-IKZF3chr1778403631chr17379227461019FFSEKRHCFTCTTTTTTAGTGAGAAGCGCCACTGCT
ENDOV-IKZF3chr1778403631chr17379227461019RWSEKRHCFATAGATGGAGTGAGAAGCGCCACTGCT
ENDOV-IKZF3chr1778403631chr17379227461119FSEKRHCFTTTTTAGTGAGAAGCGCCACTGCT
ENDOV-IKZF3chr1778403631chr17379227461120SSGEKRHCFCCTCAGGTGAGAAGCGCCACTGCTTTG
ENDOV-IKZF3chr1778403631chr1737922746918NRWSEKRHCTCAATAGATGGAGTGAGAAGCGCCACT
ENDOV-IKZF3chr1778403631chr1737922746920GESSGEKRHCFGAGAGTCCTCAGGTGAGAAGCGCCACTGCTTTG

check button mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs.
Fusion geneHchrHbpTchrTbpStart in +/-13AAEnd in +/-13AAFusionNeoAntigen peptideFusionNEoAntigen RNA sequence

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Information of the samples that have these potential fusion neoantigens of ENDOV-IKZF3

check button These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens.
Cancer typeFusion geneHchrHbpHenstTchrTbpTenstSample
BRCAENDOV-IKZF3chr1778403631ENST00000323854chr1737922746ENST00000346243TCGA-A2-A0T1-01A
BRCAENDOV-IKZF3chr1778403631ENST00000517295chr1737922746ENST00000346872TCGA-A2-A0T1-01A
BRCAENDOV-IKZF3chr1778403631ENST00000522751chr1737922746ENST00000439016TCGA-A2-A0T1-01A

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Potential target of CAR-T therapy development for ENDOV-IKZF3

check button Predicted 3D structure. We used RoseTTAFold.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features


* Minus value of BPloci means that the break point is located before the CDS.
- In-frame and retained 'Transmembrane'.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

check button Subcellular localization prediction of the transmembrane domain retained fusion proteins
* We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image.
HgeneHchrHbpHenstTgeneTchrTbpTenstDeepLoc result

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Related Drugs to ENDOV-IKZF3

check button Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDrugSourcePMID

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Related Diseases to ENDOV-IKZF3

check button Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDiseaseSourcePMID

check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource