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Center for Computational Systems Medicine
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Fusion Gene and Fusion Protein Summary

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Fusion Amino Acid Sequences (multiple BPs and multiple gene isoforms)

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Fusion Protein Breakpoint Sequences - (for the Screening of the FusionNeoAntigens)

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Potential FusionNeoAntigens in HLA I - (netMHCpan v4.1 + deepHLApan v1.1)

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Potential FusionNeoAntigens in HLA II - (netMHCIIpan v4.1)

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Fusion Breakpoint 14 AA Peptide Structure - (RoseTTAFold)

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D - (Glide)

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Vaccine Design for the FusionNeoAntigens (RNA/protein sequences)

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Potential target of CAR-T therapy development

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Information on the samples that have these potential fusion neoantigens

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Fusion Protein Targeting Drugs - (Manual Curation)

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Fusion Protein Related diseases - (Manual Curation)

Fusion Protein:ERBB2-GUK1

Fusion Gene and Fusion Protein Summary

check button Fusion gene summary
Fusion partner gene informationFusion gene name: ERBB2-GUK1
FusionPDB ID: 27149
FusionGDB2.0 ID: 27149
HgeneTgene
Gene symbol

ERBB2

GUK1

Gene ID

2064

2987

Gene nameerb-b2 receptor tyrosine kinase 2guanylate kinase 1
SynonymsCD340|HER-2|HER-2/neu|HER2|MLN 19|NEU|NGL|TKR1GMK
Cytomap

17q12

1q42.13

Type of geneprotein-codingprotein-coding
Descriptionreceptor tyrosine-protein kinase erbB-2c-erb B2/neu proteinherstatinhuman epidermal growth factor receptor 2metastatic lymph node gene 19 proteinneuro/glioblastoma derived oncogene homologneuroblastoma/glioblastoma derived oncogene homologp185erbB2guanylate kinaseATP:GMP phosphotransferaseGMP kinase
Modification date2020032920200320
UniProtAcc

P04626

Main function of 5'-partner protein: FUNCTION: Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. {ECO:0000305}.; FUNCTION: In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth. {ECO:0000269|PubMed:10358079, ECO:0000269|PubMed:15380516, ECO:0000269|PubMed:21555369}.

Q16774

Main function of 5'-partner protein: FUNCTION: Catalyzes the phosphorylation of GMP to GDP. Essential enzyme for recycling GMP and indirectly, cyclic GMP (cGMP) (PubMed:31201273). Involved in the cGMP metabolism in photoreceptors (By similarity). It may also have a role in the survival and growth progression of some tumors (PubMed:31201273). In addition to its physiological role, GUK1 is essential for convert prodrugs used for the treatment of cancers and viral infections into their pharmacologically active metabolites, most notably acyclovir, ganciclovir, and 6-thioguanine and its closely related analog 6-mercaptopurine (PubMed:197968, PubMed:6248551, PubMed:6306664). {ECO:0000250|UniProtKB:P46195, ECO:0000269|PubMed:197968, ECO:0000269|PubMed:31201273, ECO:0000269|PubMed:6248551, ECO:0000269|PubMed:6306664}.
Ensembl transtripts involved in fusion geneENST idsENST00000269571, ENST00000406381, 
ENST00000445658, ENST00000540042, 
ENST00000540147, ENST00000541774, 
ENST00000578199, ENST00000584450, 
ENST00000584601, ENST00000584888, 
ENST00000366716, ENST00000470040, 
ENST00000366721, ENST00000312726, 
ENST00000366718, ENST00000366722, 
ENST00000366723, ENST00000366726, 
ENST00000366728, ENST00000366730, 
ENST00000391865, 
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)* DoF score50 X 44 X 14=308009 X 8 X 3=216
# samples 7310
** MAII scorelog2(73/30800*10)=-5.39889007670225
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(10/216*10)=-1.11103131238874
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Fusion gene context

PubMed: ERBB2 [Title/Abstract] AND GUK1 [Title/Abstract] AND fusion [Title/Abstract]

Fusion neoantigen context

PubMed: ERBB2 [Title/Abstract] AND GUK1 [Title/Abstract] AND neoantigen [Title/Abstract]

Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)ERBB2(37872192)-GUK1(228333727), # samples:1
Anticipated loss of major functional domain due to fusion event.ERBB2-GUK1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
ERBB2-GUK1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
ERBB2-GUK1 seems lost the major protein functional domain in Hgene partner, which is a CGC due to the frame-shifted ORF.
ERBB2-GUK1 seems lost the major protein functional domain in Hgene partner, which is a IUPHAR drug target due to the frame-shifted ORF.
ERBB2-GUK1 seems lost the major protein functional domain in Hgene partner, which is a kinase due to the frame-shifted ORF.
ERBB2-GUK1 seems lost the major protein functional domain in Tgene partner, which is a cell metabolism gene due to the frame-shifted ORF.
ERBB2-GUK1 seems lost the major protein functional domain in Tgene partner, which is a essential gene due to the frame-shifted ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgeneERBB2

GO:0007165

signal transduction

10572067

HgeneERBB2

GO:0007166

cell surface receptor signaling pathway

9685399

HgeneERBB2

GO:0007169

transmembrane receptor protein tyrosine kinase signaling pathway

7514177

HgeneERBB2

GO:0014065

phosphatidylinositol 3-kinase signaling

7556068

HgeneERBB2

GO:0018108

peptidyl-tyrosine phosphorylation

12000754

HgeneERBB2

GO:0032886

regulation of microtubule-based process

20937854

HgeneERBB2

GO:0035556

intracellular signal transduction

19372587

HgeneERBB2

GO:0042060

wound healing

12646923

HgeneERBB2

GO:0043406

positive regulation of MAP kinase activity

10572067

HgeneERBB2

GO:0045785

positive regulation of cell adhesion

7556068

HgeneERBB2

GO:0046777

protein autophosphorylation

7556068

HgeneERBB2

GO:0050679

positive regulation of epithelial cell proliferation

10572067

HgeneERBB2

GO:0071363

cellular response to growth factor stimulus

20010870

HgeneERBB2

GO:0090314

positive regulation of protein targeting to membrane

20010870

TgeneGUK1

GO:0006163

purine nucleotide metabolic process

8663313

TgeneGUK1

GO:0006805

xenobiotic metabolic process

6306664



check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr17:37872192/chr1:228333727)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonRetention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here.

check buttonFusion gene breakpoints across ERBB2 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check buttonFusion gene breakpoints across GUK1 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure


Top

Fusion Amino Acid Sequences


check buttonFusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000584601ERBB2chr1737872192+ENST00000366730GUK1chr1228333727+284522207522701649
ENST00000584601ERBB2chr1737872192+ENST00000391865GUK1chr1228333727+296022207522701649
ENST00000584601ERBB2chr1737872192+ENST00000366726GUK1chr1228333727+288522207522701649
ENST00000584601ERBB2chr1737872192+ENST00000312726GUK1chr1228333727+295122207522701649
ENST00000584601ERBB2chr1737872192+ENST00000366728GUK1chr1228333727+287422207522770672
ENST00000584601ERBB2chr1737872192+ENST00000366723GUK1chr1228333727+288822207522701649
ENST00000584601ERBB2chr1737872192+ENST00000366722GUK1chr1228333727+289022207522836694
ENST00000584601ERBB2chr1737872192+ENST00000366718GUK1chr1228333727+295622207522701649
ENST00000406381ERBB2chr1737872192+ENST00000366730GUK1chr1228333727+255819335102414634
ENST00000406381ERBB2chr1737872192+ENST00000391865GUK1chr1228333727+267319335102414634
ENST00000406381ERBB2chr1737872192+ENST00000366726GUK1chr1228333727+259819335102414634
ENST00000406381ERBB2chr1737872192+ENST00000312726GUK1chr1228333727+266419335102414634
ENST00000406381ERBB2chr1737872192+ENST00000366728GUK1chr1228333727+258719335102483657
ENST00000406381ERBB2chr1737872192+ENST00000366723GUK1chr1228333727+260119335102414634
ENST00000406381ERBB2chr1737872192+ENST00000366722GUK1chr1228333727+260319335102549679
ENST00000406381ERBB2chr1737872192+ENST00000366718GUK1chr1228333727+266919335102414634
ENST00000541774ERBB2chr1737872192+ENST00000366730GUK1chr1228333727+2093146801949649
ENST00000541774ERBB2chr1737872192+ENST00000391865GUK1chr1228333727+2208146801949649
ENST00000541774ERBB2chr1737872192+ENST00000366726GUK1chr1228333727+2133146801949649
ENST00000541774ERBB2chr1737872192+ENST00000312726GUK1chr1228333727+2199146801949649
ENST00000541774ERBB2chr1737872192+ENST00000366728GUK1chr1228333727+2122146802018672
ENST00000541774ERBB2chr1737872192+ENST00000366723GUK1chr1228333727+2136146801949649
ENST00000541774ERBB2chr1737872192+ENST00000366722GUK1chr1228333727+2138146802084694
ENST00000541774ERBB2chr1737872192+ENST00000366718GUK1chr1228333727+2204146801949649
ENST00000445658ERBB2chr1737872192+ENST00000366730GUK1chr1228333727+1573948501429459
ENST00000445658ERBB2chr1737872192+ENST00000391865GUK1chr1228333727+1688948501429459
ENST00000445658ERBB2chr1737872192+ENST00000366726GUK1chr1228333727+1613948501429459
ENST00000445658ERBB2chr1737872192+ENST00000312726GUK1chr1228333727+1679948501429459
ENST00000445658ERBB2chr1737872192+ENST00000366728GUK1chr1228333727+1602948501498482
ENST00000445658ERBB2chr1737872192+ENST00000366723GUK1chr1228333727+1616948501429459
ENST00000445658ERBB2chr1737872192+ENST00000366722GUK1chr1228333727+1618948501564504
ENST00000445658ERBB2chr1737872192+ENST00000366718GUK1chr1228333727+1684948501429459
ENST00000540147ERBB2chr1737872192+ENST00000366730GUK1chr1228333727+23761751252232735
ENST00000540147ERBB2chr1737872192+ENST00000391865GUK1chr1228333727+24911751252232735
ENST00000540147ERBB2chr1737872192+ENST00000366726GUK1chr1228333727+24161751252232735
ENST00000540147ERBB2chr1737872192+ENST00000312726GUK1chr1228333727+24821751252232735
ENST00000540147ERBB2chr1737872192+ENST00000366728GUK1chr1228333727+24051751252301758
ENST00000540147ERBB2chr1737872192+ENST00000366723GUK1chr1228333727+24191751252232735
ENST00000540147ERBB2chr1737872192+ENST00000366722GUK1chr1228333727+24211751252367780
ENST00000540147ERBB2chr1737872192+ENST00000366718GUK1chr1228333727+24871751252232735
ENST00000584450ERBB2chr1737872192+ENST00000366730GUK1chr1228333727+2353172822209735
ENST00000584450ERBB2chr1737872192+ENST00000391865GUK1chr1228333727+2468172822209735
ENST00000584450ERBB2chr1737872192+ENST00000366726GUK1chr1228333727+2393172822209735
ENST00000584450ERBB2chr1737872192+ENST00000312726GUK1chr1228333727+2459172822209735
ENST00000584450ERBB2chr1737872192+ENST00000366728GUK1chr1228333727+2382172822278758
ENST00000584450ERBB2chr1737872192+ENST00000366723GUK1chr1228333727+2396172822209735
ENST00000584450ERBB2chr1737872192+ENST00000366722GUK1chr1228333727+2398172822344780
ENST00000584450ERBB2chr1737872192+ENST00000366718GUK1chr1228333727+2464172822209735
ENST00000269571ERBB2chr1737872192+ENST00000366730GUK1chr1228333727+22971672572153698
ENST00000269571ERBB2chr1737872192+ENST00000391865GUK1chr1228333727+24121672572153698
ENST00000269571ERBB2chr1737872192+ENST00000366726GUK1chr1228333727+23371672572153698
ENST00000269571ERBB2chr1737872192+ENST00000312726GUK1chr1228333727+24031672572153698
ENST00000269571ERBB2chr1737872192+ENST00000366728GUK1chr1228333727+23261672572222721
ENST00000269571ERBB2chr1737872192+ENST00000366723GUK1chr1228333727+23401672572153698
ENST00000269571ERBB2chr1737872192+ENST00000366722GUK1chr1228333727+23421672572288743
ENST00000269571ERBB2chr1737872192+ENST00000366718GUK1chr1228333727+24081672572153698
ENST00000578199ERBB2chr1737872192+ENST00000366730GUK1chr1228333727+258919645412445634
ENST00000578199ERBB2chr1737872192+ENST00000391865GUK1chr1228333727+270419645412445634
ENST00000578199ERBB2chr1737872192+ENST00000366726GUK1chr1228333727+262919645412445634
ENST00000578199ERBB2chr1737872192+ENST00000312726GUK1chr1228333727+269519645412445634
ENST00000578199ERBB2chr1737872192+ENST00000366728GUK1chr1228333727+261819645412514657
ENST00000578199ERBB2chr1737872192+ENST00000366723GUK1chr1228333727+263219645412445634
ENST00000578199ERBB2chr1737872192+ENST00000366722GUK1chr1228333727+263419645412580679
ENST00000578199ERBB2chr1737872192+ENST00000366718GUK1chr1228333727+270019645412445634
ENST00000540042ERBB2chr1737872192+ENST00000366730GUK1chr1228333727+221415891662070634
ENST00000540042ERBB2chr1737872192+ENST00000391865GUK1chr1228333727+232915891662070634
ENST00000540042ERBB2chr1737872192+ENST00000366726GUK1chr1228333727+225415891662070634
ENST00000540042ERBB2chr1737872192+ENST00000312726GUK1chr1228333727+232015891662070634
ENST00000540042ERBB2chr1737872192+ENST00000366728GUK1chr1228333727+224315891662139657
ENST00000540042ERBB2chr1737872192+ENST00000366723GUK1chr1228333727+225715891662070634
ENST00000540042ERBB2chr1737872192+ENST00000366722GUK1chr1228333727+225915891662205679
ENST00000540042ERBB2chr1737872192+ENST00000366718GUK1chr1228333727+232515891662070634

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000584601ENST00000366730ERBB2chr1737872192+GUK1chr1228333727+0.0307758770.96922415
ENST00000584601ENST00000391865ERBB2chr1737872192+GUK1chr1228333727+0.0253884850.9746115
ENST00000584601ENST00000366726ERBB2chr1737872192+GUK1chr1228333727+0.029441070.97055894
ENST00000584601ENST00000312726ERBB2chr1737872192+GUK1chr1228333727+0.0256505350.97434944
ENST00000584601ENST00000366728ERBB2chr1737872192+GUK1chr1228333727+0.0391844140.9608156
ENST00000584601ENST00000366723ERBB2chr1737872192+GUK1chr1228333727+0.0493947640.9506052
ENST00000584601ENST00000366722ERBB2chr1737872192+GUK1chr1228333727+0.0345206150.9654794
ENST00000584601ENST00000366718ERBB2chr1737872192+GUK1chr1228333727+0.0259846410.97401536
ENST00000406381ENST00000366730ERBB2chr1737872192+GUK1chr1228333727+0.0231071670.9768928
ENST00000406381ENST00000391865ERBB2chr1737872192+GUK1chr1228333727+0.0191737710.98082626
ENST00000406381ENST00000366726ERBB2chr1737872192+GUK1chr1228333727+0.0223293790.97767067
ENST00000406381ENST00000312726ERBB2chr1737872192+GUK1chr1228333727+0.0193340090.980666
ENST00000406381ENST00000366728ERBB2chr1737872192+GUK1chr1228333727+0.0365788680.9634211
ENST00000406381ENST00000366723ERBB2chr1737872192+GUK1chr1228333727+0.043056130.95694387
ENST00000406381ENST00000366722ERBB2chr1737872192+GUK1chr1228333727+0.031396520.9686035
ENST00000406381ENST00000366718ERBB2chr1737872192+GUK1chr1228333727+0.019690980.980309
ENST00000541774ENST00000366730ERBB2chr1737872192+GUK1chr1228333727+0.0458298440.95417017
ENST00000541774ENST00000391865ERBB2chr1737872192+GUK1chr1228333727+0.0376917120.9623083
ENST00000541774ENST00000366726ERBB2chr1737872192+GUK1chr1228333727+0.0446000730.95539993
ENST00000541774ENST00000312726ERBB2chr1737872192+GUK1chr1228333727+0.0380567720.96194327
ENST00000541774ENST00000366728ERBB2chr1737872192+GUK1chr1228333727+0.0590506980.94094926
ENST00000541774ENST00000366723ERBB2chr1737872192+GUK1chr1228333727+0.071804510.92819554
ENST00000541774ENST00000366722ERBB2chr1737872192+GUK1chr1228333727+0.0507872960.9492127
ENST00000541774ENST00000366718ERBB2chr1737872192+GUK1chr1228333727+0.0387251530.9612748
ENST00000445658ENST00000366730ERBB2chr1737872192+GUK1chr1228333727+0.0110657620.9889342
ENST00000445658ENST00000391865ERBB2chr1737872192+GUK1chr1228333727+0.012884970.987115
ENST00000445658ENST00000366726ERBB2chr1737872192+GUK1chr1228333727+0.0123271050.98767287
ENST00000445658ENST00000312726ERBB2chr1737872192+GUK1chr1228333727+0.0129354180.9870646
ENST00000445658ENST00000366728ERBB2chr1737872192+GUK1chr1228333727+0.083653590.91634643
ENST00000445658ENST00000366723ERBB2chr1737872192+GUK1chr1228333727+0.0507147540.94928527
ENST00000445658ENST00000366722ERBB2chr1737872192+GUK1chr1228333727+0.0373467580.9626533
ENST00000445658ENST00000366718ERBB2chr1737872192+GUK1chr1228333727+0.0129539160.9870461
ENST00000540147ENST00000366730ERBB2chr1737872192+GUK1chr1228333727+0.0482063550.9517937
ENST00000540147ENST00000391865ERBB2chr1737872192+GUK1chr1228333727+0.038885350.9611147
ENST00000540147ENST00000366726ERBB2chr1737872192+GUK1chr1228333727+0.0456058420.9543941
ENST00000540147ENST00000312726ERBB2chr1737872192+GUK1chr1228333727+0.039293950.9607061
ENST00000540147ENST00000366728ERBB2chr1737872192+GUK1chr1228333727+0.0579203670.94207966
ENST00000540147ENST00000366723ERBB2chr1737872192+GUK1chr1228333727+0.071692870.9283071
ENST00000540147ENST00000366722ERBB2chr1737872192+GUK1chr1228333727+0.050164970.94983506
ENST00000540147ENST00000366718ERBB2chr1737872192+GUK1chr1228333727+0.039861130.9601389
ENST00000584450ENST00000366730ERBB2chr1737872192+GUK1chr1228333727+0.0502894070.94971055
ENST00000584450ENST00000391865ERBB2chr1737872192+GUK1chr1228333727+0.0410876830.9589123
ENST00000584450ENST00000366726ERBB2chr1737872192+GUK1chr1228333727+0.0478796770.95212024
ENST00000584450ENST00000312726ERBB2chr1737872192+GUK1chr1228333727+0.041445430.95855457
ENST00000584450ENST00000366728ERBB2chr1737872192+GUK1chr1228333727+0.0612633940.9387367
ENST00000584450ENST00000366723ERBB2chr1737872192+GUK1chr1228333727+0.075093460.9249065
ENST00000584450ENST00000366722ERBB2chr1737872192+GUK1chr1228333727+0.0529416650.9470583
ENST00000584450ENST00000366718ERBB2chr1737872192+GUK1chr1228333727+0.0419535560.9580465
ENST00000269571ENST00000366730ERBB2chr1737872192+GUK1chr1228333727+0.0510696320.9489304
ENST00000269571ENST00000391865ERBB2chr1737872192+GUK1chr1228333727+0.0418842470.95811576
ENST00000269571ENST00000366726ERBB2chr1737872192+GUK1chr1228333727+0.0488354270.9511646
ENST00000269571ENST00000312726ERBB2chr1737872192+GUK1chr1228333727+0.0422961670.95770377
ENST00000269571ENST00000366728ERBB2chr1737872192+GUK1chr1228333727+0.062684230.9373158
ENST00000269571ENST00000366723ERBB2chr1737872192+GUK1chr1228333727+0.076886390.9231136
ENST00000269571ENST00000366722ERBB2chr1737872192+GUK1chr1228333727+0.054469670.94553035
ENST00000269571ENST00000366718ERBB2chr1737872192+GUK1chr1228333727+0.0427477170.9572523
ENST00000578199ENST00000366730ERBB2chr1737872192+GUK1chr1228333727+0.0190459080.98095405
ENST00000578199ENST00000391865ERBB2chr1737872192+GUK1chr1228333727+0.0159368050.98406315
ENST00000578199ENST00000366726ERBB2chr1737872192+GUK1chr1228333727+0.0183741880.9816258
ENST00000578199ENST00000312726ERBB2chr1737872192+GUK1chr1228333727+0.0160596430.98394036
ENST00000578199ENST00000366728ERBB2chr1737872192+GUK1chr1228333727+0.0304645130.96953547
ENST00000578199ENST00000366723ERBB2chr1737872192+GUK1chr1228333727+0.0363251860.9636748
ENST00000578199ENST00000366722ERBB2chr1737872192+GUK1chr1228333727+0.0263164580.97368354
ENST00000578199ENST00000366718ERBB2chr1737872192+GUK1chr1228333727+0.0163853490.9836146
ENST00000540042ENST00000366730ERBB2chr1737872192+GUK1chr1228333727+0.0263991450.9736008
ENST00000540042ENST00000391865ERBB2chr1737872192+GUK1chr1228333727+0.0202953630.9797046
ENST00000540042ENST00000366726ERBB2chr1737872192+GUK1chr1228333727+0.0246304530.9753695
ENST00000540042ENST00000312726ERBB2chr1737872192+GUK1chr1228333727+0.0205561020.9794439
ENST00000540042ENST00000366728ERBB2chr1737872192+GUK1chr1228333727+0.0395578070.96044225
ENST00000540042ENST00000366723ERBB2chr1737872192+GUK1chr1228333727+0.0476574260.9523426
ENST00000540042ENST00000366722ERBB2chr1737872192+GUK1chr1228333727+0.0346219280.96537805
ENST00000540042ENST00000366718ERBB2chr1737872192+GUK1chr1228333727+0.0209366880.97906333

check button Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones.

Get the fusion protein sequences from here.

Fusion protein sequence information is available in the fasta format.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

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Fusion Protein Breakpoint Sequences for ERBB2-GUK1

check button +/-13 AA sequence from the breakpoints of the fusion protein sequences.
HgeneHchrHbpTgeneTchrTbpLength(fusion protein)BP in fusion proteinPeptide
ERBB2chr1737872192GUK1chr12283337271468489ALLHTANRPEDECDTTRNPRPGEENG
ERBB2chr1737872192GUK1chr12283337271589474ALLHTANRPEDECDTTRNPRPGEENG
ERBB2chr1737872192GUK1chr12283337271672538ALLHTANRPEDECDTTRNPRPGEENG
ERBB2chr1737872192GUK1chr12283337271728575ALLHTANRPEDECDTTRNPRPGEENG
ERBB2chr1737872192GUK1chr12283337271751575ALLHTANRPEDECDTTRNPRPGEENG
ERBB2chr1737872192GUK1chr12283337271933474ALLHTANRPEDECDTTRNPRPGEENG
ERBB2chr1737872192GUK1chr12283337271964474ALLHTANRPEDECDTTRNPRPGEENG
ERBB2chr1737872192GUK1chr12283337272220489ALLHTANRPEDECDTTRNPRPGEENG
ERBB2chr1737872192GUK1chr1228333727948299ALLHTANRPEDECDTTRNPRPGEENG

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Potential FusionNeoAntigen Information of ERBB2-GUK1 in HLA I

check button Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.

check button Potential FusionNeoAntigen Information
* We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5)
Fusion geneHchrHbpTgeneTchrTbpHLA IFusionNeoAntigen peptideBinding scoreImmunogenic scoreNeoantigen start (at BP 13)Neoantigen end (at BP 13)

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Potential FusionNeoAntigen Information of ERBB2-GUK1 in HLA II

check button Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.

check button Potential FusionNeoAntigen Information
* We used NetMHCIIpan v4.1 (%rank<0.5).
Fusion geneHchrHbpTgeneTchrTbpHLA IIFusionNeoAntigen peptideNeoantigen start (at BP 13)Neoantigen end (at BP 13)

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Fusion breakpoint peptide structures of ERBB2-GUK1

check button3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens
* The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA.

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of ERBB2-GUK1

check buttonVirtual screening between 25 HLAs (from PDB) and FusionNeoAntigens
* We used Glide to predict the interaction between HLAs and neoantigens.
HLA allelePDB IDFile nameBPseqDocking scoreGlide score

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Vaccine Design for the FusionNeoAntigens of ERBB2-GUK1

check button mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is.
Fusion geneHchrHbpTchrTbpStart in +/-13AAEnd in +/-13AAFusionNeoAntigen peptide sequenceFusionNeoAntigen RNA sequence

check button mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs.
Fusion geneHchrHbpTchrTbpStart in +/-13AAEnd in +/-13AAFusionNeoAntigen peptideFusionNEoAntigen RNA sequence

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Information of the samples that have these potential fusion neoantigens of ERBB2-GUK1

check button These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens.
Cancer typeFusion geneHchrHbpHenstTchrTbpTenstSample

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Potential target of CAR-T therapy development for ERBB2-GUK1

check button Predicted 3D structure. We used RoseTTAFold.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features


* Minus value of BPloci means that the break point is located before the CDS.
- In-frame and retained 'Transmembrane'.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

check button Subcellular localization prediction of the transmembrane domain retained fusion proteins
* We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image.
HgeneHchrHbpHenstTgeneTchrTbpTenstDeepLoc result

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Related Drugs to ERBB2-GUK1

check button Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDrugSourcePMID

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Related Diseases to ERBB2-GUK1

check button Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDiseaseSourcePMID

check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource