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Center for Computational Systems Medicine
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Fusion Gene and Fusion Protein Summary

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Fusion Amino Acid Sequences (multiple BPs and multiple gene isoforms)

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Fusion Protein Breakpoint Sequences - (for the Screening of the FusionNeoAntigens)

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Potential FusionNeoAntigens in HLA I - (netMHCpan v4.1 + deepHLApan v1.1)

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Potential FusionNeoAntigens in HLA II - (netMHCIIpan v4.1)

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Fusion Breakpoint 14 AA Peptide Structure - (RoseTTAFold)

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D - (Glide)

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Vaccine Design for the FusionNeoAntigens (RNA/protein sequences)

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Potential target of CAR-T therapy development

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Information on the samples that have these potential fusion neoantigens

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Fusion Protein Targeting Drugs - (Manual Curation)

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Fusion Protein Related diseases - (Manual Curation)

Fusion Protein:ESCO2-EPHX2

Fusion Gene and Fusion Protein Summary

check button Fusion gene summary
Fusion partner gene informationFusion gene name: ESCO2-EPHX2
FusionPDB ID: 27516
FusionGDB2.0 ID: 27516
HgeneTgene
Gene symbol

ESCO2

EPHX2

Gene ID

157570

2053

Gene nameestablishment of sister chromatid cohesion N-acetyltransferase 2epoxide hydrolase 2
Synonyms2410004I17Rik|EFO2|EFO2p|RBS|hEFO2ABHD20|CEH|SEH
Cytomap

8p21.1

8p21.2-p21.1

Type of geneprotein-codingprotein-coding
DescriptionN-acetyltransferase ESCO2ECO1 homolog 2establishment factor-like protein 2bifunctional epoxide hydrolase 2epoxide hydrataseepoxide hydrolase 2, cytoplasmicepoxide hydrolase 2, cytosolicepoxide hydrolase, soluble
Modification date2020032820200327
UniProtAcc

Q56NI9

Main function of 5'-partner protein: FUNCTION: Acetyltransferase required for the establishment of sister chromatid cohesion (PubMed:15821733, PubMed:15958495). Couples the processes of cohesion and DNA replication to ensure that only sister chromatids become paired together. In contrast to the structural cohesins, the deposition and establishment factors are required only during the S phase. Acetylates the cohesin component SMC3 (PubMed:21111234). {ECO:0000269|PubMed:15821733, ECO:0000269|PubMed:15958495, ECO:0000269|PubMed:19907496, ECO:0000269|PubMed:21111234}.

P34913

Main function of 5'-partner protein: FUNCTION: Bifunctional enzyme (PubMed:12574510). The C-terminal domain has epoxide hydrolase activity and acts on epoxides (alkene oxides, oxiranes) and arene oxides (PubMed:12869654, PubMed:12574510, PubMed:22798687). Plays a role in xenobiotic metabolism by degrading potentially toxic epoxides (By similarity). Also determines steady-state levels of physiological mediators (PubMed:12869654, PubMed:12574510, PubMed:22798687, PubMed:21217101). {ECO:0000250|UniProtKB:P80299, ECO:0000269|PubMed:12574508, ECO:0000269|PubMed:12574510, ECO:0000269|PubMed:12869654, ECO:0000269|PubMed:21217101, ECO:0000269|PubMed:22798687}.; FUNCTION: Bifunctional enzyme (PubMed:12574510). The N-terminal domain has lipid phosphatase activity, with the highest activity towards threo-9,10-phosphonooxy-hydroxy-octadecanoic acid, followed by erythro-9,10-phosphonooxy-hydroxy-octadecanoic acid, 12-phosphonooxy-octadec-9Z-enoic acid and 12-phosphonooxy-octadec-9E-enoic acid (PubMed:12574510). Has phosphatase activity toward lyso-glycerophospholipids with also some lower activity toward lysolipids of sphingolipid and isoprenoid phosphates (PubMed:22217705, PubMed:22387545). {ECO:0000269|PubMed:12574510, ECO:0000269|PubMed:22217705, ECO:0000269|PubMed:22387545}.
Ensembl transtripts involved in fusion geneENST idsENST00000305188, ENST00000397418, 
ENST00000523910, 		ENST00000520666, 
ENST00000380476, ENST00000517536, 
ENST00000518379, ENST00000521400, 
ENST00000521780, 
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)* DoF score3 X 3 X 3=274 X 4 X 3=48
# samples 34
** MAII scorelog2(3/27*10)=0.15200309344505
effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs).
DoF>8 and MAII>0		log2(4/48*10)=-0.263034405833794
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Fusion gene context

PubMed: ESCO2 [Title/Abstract] AND EPHX2 [Title/Abstract] AND fusion [Title/Abstract]

Fusion neoantigen context

PubMed: ESCO2 [Title/Abstract] AND EPHX2 [Title/Abstract] AND neoantigen [Title/Abstract]

Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)ESCO2(27646495)-EPHX2(27394301), # samples:1
Anticipated loss of major functional domain due to fusion event.ESCO2-EPHX2 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
ESCO2-EPHX2 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
ESCO2-EPHX2 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
ESCO2-EPHX2 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
TgeneEPHX2

GO:0010628

positive regulation of gene expression

18974052

TgeneEPHX2

GO:0016311

dephosphorylation

12574508

TgeneEPHX2

GO:0042632

cholesterol homeostasis

18974052

TgeneEPHX2

GO:0046272

stilbene catabolic process

8342951

TgeneEPHX2

GO:0046839

phospholipid dephosphorylation

12574510

TgeneEPHX2

GO:0097176

epoxide metabolic process

22798687



check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr8:27646495/chr8:27394301)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonRetention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here.

check buttonFusion gene breakpoints across ESCO2 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check buttonFusion gene breakpoints across EPHX2 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure


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Fusion Amino Acid Sequences


check buttonFusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000305188ESCO2chr827646495-ENST00000521400EPHX2chr827394301+30411501311998655
ENST00000305188ESCO2chr827646495-ENST00000517536EPHX2chr827394301+23961501311998655
ENST00000305188ESCO2chr827646495-ENST00000521780EPHX2chr827394301+23961501311998655
ENST00000305188ESCO2chr827646495-ENST00000380476EPHX2chr827394301+23901501311998655
ENST00000305188ESCO2chr827646495-ENST00000518379EPHX2chr827394301+23691501311998655
ENST00000397418ESCO2chr827646495-ENST00000521400EPHX2chr827394301+2721118113942309
ENST00000397418ESCO2chr827646495-ENST00000517536EPHX2chr827394301+2076118113942309
ENST00000397418ESCO2chr827646495-ENST00000521780EPHX2chr827394301+2076118113942309
ENST00000397418ESCO2chr827646495-ENST00000380476EPHX2chr827394301+2070118113942309
ENST00000397418ESCO2chr827646495-ENST00000518379EPHX2chr827394301+2049118113942309

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000305188ENST00000521400ESCO2chr827646495-EPHX2chr827394301+0.0023568470.9976432
ENST00000305188ENST00000517536ESCO2chr827646495-EPHX2chr827394301+0.0018086810.9981913
ENST00000305188ENST00000521780ESCO2chr827646495-EPHX2chr827394301+0.0018086810.9981913
ENST00000305188ENST00000380476ESCO2chr827646495-EPHX2chr827394301+0.0018056760.9981943
ENST00000305188ENST00000518379ESCO2chr827646495-EPHX2chr827394301+0.0018717960.99812824
ENST00000397418ENST00000521400ESCO2chr827646495-EPHX2chr827394301+0.0069647680.9930353
ENST00000397418ENST00000517536ESCO2chr827646495-EPHX2chr827394301+0.0065717630.9934282
ENST00000397418ENST00000521780ESCO2chr827646495-EPHX2chr827394301+0.0065717630.9934282
ENST00000397418ENST00000380476ESCO2chr827646495-EPHX2chr827394301+0.0067974090.99320257
ENST00000397418ENST00000518379ESCO2chr827646495-EPHX2chr827394301+0.0069027330.99309725

check button Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones.

Get the fusion protein sequences from here.

Fusion protein sequence information is available in the fasta format.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

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Fusion Protein Breakpoint Sequences for ESCO2-EPHX2

check button +/-13 AA sequence from the breakpoints of the fusion protein sequences.
HgeneHchrHbpTgeneTchrTbpLength(fusion protein)BP in fusion proteinPeptide
ESCO2chr827646495EPHX2chr8273943011501490QHHHRFLEGIKYVGVAEAELEQNLSR

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Potential FusionNeoAntigen Information of ESCO2-EPHX2 in HLA I

check button Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.
ESCO2-EPHX2_27646495_27394301.msa

check button Potential FusionNeoAntigen Information
* We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5)
Fusion geneHchrHbpTgeneTchrTbpHLA IFusionNeoAntigen peptideBinding scoreImmunogenic scoreNeoantigen start (at BP 13)Neoantigen end (at BP 13)
ESCO2-EPHX2chr827646495chr8273943011501HLA-B39:06IKYVGVAEA0.98990.7971918
ESCO2-EPHX2chr827646495chr8273943011501HLA-C03:06YVGVAEAEL0.96480.98951120

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Potential FusionNeoAntigen Information of ESCO2-EPHX2 in HLA II

check button Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.
ESCO2-EPHX2_27646495_27394301.msa

check button Potential FusionNeoAntigen Information
* We used NetMHCIIpan v4.1 (%rank<0.5).
Fusion geneHchrHbpTgeneTchrTbpHLA IIFusionNeoAntigen peptideNeoantigen start (at BP 13)Neoantigen end (at BP 13)
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0403LEGIKYVGVAEAELE621
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0403EGIKYVGVAEAELEQ722
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0403RFLEGIKYVGVAEAE419
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0403FLEGIKYVGVAEAEL520
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0413LEGIKYVGVAEAELE621
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0415LEGIKYVGVAEAELE621
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0415EGIKYVGVAEAELEQ722
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0415FLEGIKYVGVAEAEL520
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0427LEGIKYVGVAEAELE621
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0427EGIKYVGVAEAELEQ722
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0431LEGIKYVGVAEAELE621
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0436LEGIKYVGVAEAELE621
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0436EGIKYVGVAEAELEQ722
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0436FLEGIKYVGVAEAEL520
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0439LEGIKYVGVAEAELE621
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0439EGIKYVGVAEAELEQ722
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0439RFLEGIKYVGVAEAE419
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0439FLEGIKYVGVAEAEL520
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0440LEGIKYVGVAEAELE621
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0440EGIKYVGVAEAELEQ722
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0440FLEGIKYVGVAEAEL520
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0440RFLEGIKYVGVAEAE419
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0441LEGIKYVGVAEAELE621
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0441EGIKYVGVAEAELEQ722
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0441RFLEGIKYVGVAEAE419
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0441FLEGIKYVGVAEAEL520
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0442LEGIKYVGVAEAELE621
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0442FLEGIKYVGVAEAEL520
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0442EGIKYVGVAEAELEQ722
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0444LEGIKYVGVAEAELE621
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0444FLEGIKYVGVAEAEL520
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0444EGIKYVGVAEAELEQ722
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0446LEGIKYVGVAEAELE621
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0446EGIKYVGVAEAELEQ722
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0446RFLEGIKYVGVAEAE419
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0446FLEGIKYVGVAEAEL520
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0449LEGIKYVGVAEAELE621
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0449EGIKYVGVAEAELEQ722
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0449FLEGIKYVGVAEAEL520
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0449RFLEGIKYVGVAEAE419
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0450LEGIKYVGVAEAELE621
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0450EGIKYVGVAEAELEQ722
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0451LEGIKYVGVAEAELE621
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0451EGIKYVGVAEAELEQ722
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0451RFLEGIKYVGVAEAE419
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0451FLEGIKYVGVAEAEL520
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0452LEGIKYVGVAEAELE621
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0452EGIKYVGVAEAELEQ722
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0452RFLEGIKYVGVAEAE419
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0452FLEGIKYVGVAEAEL520
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0453LEGIKYVGVAEAELE621
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0453FLEGIKYVGVAEAEL520
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0453EGIKYVGVAEAELEQ722
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0453RFLEGIKYVGVAEAE419
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0454LEGIKYVGVAEAELE621
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0455LEGIKYVGVAEAELE621
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0455EGIKYVGVAEAELEQ722
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0455FLEGIKYVGVAEAEL520
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0455RFLEGIKYVGVAEAE419
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0456LEGIKYVGVAEAELE621
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0456FLEGIKYVGVAEAEL520
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0456EGIKYVGVAEAELEQ722
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0456RFLEGIKYVGVAEAE419
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0458LEGIKYVGVAEAELE621
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0458EGIKYVGVAEAELEQ722
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0458FLEGIKYVGVAEAEL520
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0458RFLEGIKYVGVAEAE419
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0459LEGIKYVGVAEAELE621
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0459EGIKYVGVAEAELEQ722
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0460LEGIKYVGVAEAELE621
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0460EGIKYVGVAEAELEQ722
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0460RFLEGIKYVGVAEAE419
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0460FLEGIKYVGVAEAEL520
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0465LEGIKYVGVAEAELE621
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0465EGIKYVGVAEAELEQ722
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0465RFLEGIKYVGVAEAE419
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0465FLEGIKYVGVAEAEL520
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0468LEGIKYVGVAEAELE621
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0468EGIKYVGVAEAELEQ722
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0468FLEGIKYVGVAEAEL520
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0468RFLEGIKYVGVAEAE419
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0469EGIKYVGVAEAELEQ722
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0469LEGIKYVGVAEAELE621
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0470LEGIKYVGVAEAELE621
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0470EGIKYVGVAEAELEQ722
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0470FLEGIKYVGVAEAEL520
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0470RFLEGIKYVGVAEAE419
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0471LEGIKYVGVAEAELE621
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0471EGIKYVGVAEAELEQ722
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0471RFLEGIKYVGVAEAE419
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0471FLEGIKYVGVAEAEL520
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0473LEGIKYVGVAEAELE621
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0478LEGIKYVGVAEAELE621
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0478EGIKYVGVAEAELEQ722
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0478RFLEGIKYVGVAEAE419
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0478FLEGIKYVGVAEAEL520
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0479LEGIKYVGVAEAELE621
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0479FLEGIKYVGVAEAEL520
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0479EGIKYVGVAEAELEQ722
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0479RFLEGIKYVGVAEAE419
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0485LEGIKYVGVAEAELE621
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0485EGIKYVGVAEAELEQ722
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0485RFLEGIKYVGVAEAE419
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0485FLEGIKYVGVAEAEL520
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0488LEGIKYVGVAEAELE621
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0488FLEGIKYVGVAEAEL520
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0488EGIKYVGVAEAELEQ722
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0488RFLEGIKYVGVAEAE419
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0706GIKYVGVAEAELEQN823
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0807GIKYVGVAEAELEQN823
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0807EGIKYVGVAEAELEQ722
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0901GIKYVGVAEAELEQN823
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0901EGIKYVGVAEAELEQ722
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0902GIKYVGVAEAELEQN823
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0903GIKYVGVAEAELEQN823
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0903EGIKYVGVAEAELEQ722
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0904GIKYVGVAEAELEQN823
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0904EGIKYVGVAEAELEQ722
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0905GIKYVGVAEAELEQN823
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0905EGIKYVGVAEAELEQ722
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0907GIKYVGVAEAELEQN823
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0907EGIKYVGVAEAELEQ722
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0908GIKYVGVAEAELEQN823
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0908EGIKYVGVAEAELEQ722
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0909GIKYVGVAEAELEQN823
ESCO2-EPHX2chr827646495chr8273943011501DRB1-0909EGIKYVGVAEAELEQ722
ESCO2-EPHX2chr827646495chr8273943011501DRB1-1002LEGIKYVGVAEAELE621
ESCO2-EPHX2chr827646495chr8273943011501DRB1-1410LEGIKYVGVAEAELE621
ESCO2-EPHX2chr827646495chr8273943011501DRB1-1410EGIKYVGVAEAELEQ722
ESCO2-EPHX2chr827646495chr8273943011501DRB1-1410RFLEGIKYVGVAEAE419
ESCO2-EPHX2chr827646495chr8273943011501DRB1-1410FLEGIKYVGVAEAEL520
ESCO2-EPHX2chr827646495chr8273943011501DRB1-1527GIKYVGVAEAELEQN823
ESCO2-EPHX2chr827646495chr8273943011501DRB1-1534GIKYVGVAEAELEQN823
ESCO2-EPHX2chr827646495chr8273943011501DRB1-1601GIKYVGVAEAELEQN823
ESCO2-EPHX2chr827646495chr8273943011501DRB1-1602GIKYVGVAEAELEQN823
ESCO2-EPHX2chr827646495chr8273943011501DRB1-1603GIKYVGVAEAELEQN823
ESCO2-EPHX2chr827646495chr8273943011501DRB1-1604GIKYVGVAEAELEQN823
ESCO2-EPHX2chr827646495chr8273943011501DRB1-1604EGIKYVGVAEAELEQ722
ESCO2-EPHX2chr827646495chr8273943011501DRB1-1605GIKYVGVAEAELEQN823
ESCO2-EPHX2chr827646495chr8273943011501DRB1-1607GIKYVGVAEAELEQN823
ESCO2-EPHX2chr827646495chr8273943011501DRB1-1608GIKYVGVAEAELEQN823
ESCO2-EPHX2chr827646495chr8273943011501DRB1-1609GIKYVGVAEAELEQN823
ESCO2-EPHX2chr827646495chr8273943011501DRB1-1610GIKYVGVAEAELEQN823
ESCO2-EPHX2chr827646495chr8273943011501DRB1-1611GIKYVGVAEAELEQN823
ESCO2-EPHX2chr827646495chr8273943011501DRB1-1612GIKYVGVAEAELEQN823
ESCO2-EPHX2chr827646495chr8273943011501DRB1-1614GIKYVGVAEAELEQN823
ESCO2-EPHX2chr827646495chr8273943011501DRB1-1614EGIKYVGVAEAELEQ722
ESCO2-EPHX2chr827646495chr8273943011501DRB1-1616GIKYVGVAEAELEQN823

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Fusion breakpoint peptide structures of ESCO2-EPHX2

check button3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens
* The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA.
File nameBPseqHgeneTgeneHchrHbpTchrTbpAAlen
4891LEGIKYVGVAEAELESCO2EPHX2chr827646495chr8273943011501

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of ESCO2-EPHX2

check buttonVirtual screening between 25 HLAs (from PDB) and FusionNeoAntigens
* We used Glide to predict the interaction between HLAs and neoantigens.
HLA allelePDB IDFile nameBPseqDocking scoreGlide score
HLA-B14:023BVN4891LEGIKYVGVAEAEL-7.15543-7.26883
HLA-B14:023BVN4891LEGIKYVGVAEAEL-4.77435-5.80965
HLA-B52:013W394891LEGIKYVGVAEAEL-6.80875-6.92215
HLA-B52:013W394891LEGIKYVGVAEAEL-4.20386-5.23916
HLA-A11:014UQ24891LEGIKYVGVAEAEL-7.5194-8.5547
HLA-A11:014UQ24891LEGIKYVGVAEAEL-6.9601-7.0735
HLA-A24:025HGA4891LEGIKYVGVAEAEL-7.52403-7.63743
HLA-A24:025HGA4891LEGIKYVGVAEAEL-5.82433-6.85963
HLA-B27:056PYJ4891LEGIKYVGVAEAEL-3.28285-4.31815
HLA-B44:053DX84891LEGIKYVGVAEAEL-5.91172-6.94702
HLA-B44:053DX84891LEGIKYVGVAEAEL-4.24346-4.35686

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Vaccine Design for the FusionNeoAntigens of ESCO2-EPHX2

check button mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is.
Fusion geneHchrHbpTchrTbpStart in +/-13AAEnd in +/-13AAFusionNeoAntigen peptide sequenceFusionNeoAntigen RNA sequence
ESCO2-EPHX2chr827646495chr8273943011120YVGVAEAELTATGTGGGAGTGGCTGAGGCTGAACTG
ESCO2-EPHX2chr827646495chr827394301918IKYVGVAEAATCAAATATGTGGGAGTGGCTGAGGCT

check button mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs.
Fusion geneHchrHbpTchrTbpStart in +/-13AAEnd in +/-13AAFusionNeoAntigen peptideFusionNEoAntigen RNA sequence
ESCO2-EPHX2chr827646495chr827394301419RFLEGIKYVGVAEAEAGGTTTCTGGAAGGAATCAAATATGTGGGAGTGGCTGAGGCTGAA
ESCO2-EPHX2chr827646495chr827394301520FLEGIKYVGVAEAELTTTCTGGAAGGAATCAAATATGTGGGAGTGGCTGAGGCTGAACTG
ESCO2-EPHX2chr827646495chr827394301621LEGIKYVGVAEAELECTGGAAGGAATCAAATATGTGGGAGTGGCTGAGGCTGAACTGGAA
ESCO2-EPHX2chr827646495chr827394301722EGIKYVGVAEAELEQGAAGGAATCAAATATGTGGGAGTGGCTGAGGCTGAACTGGAACAG
ESCO2-EPHX2chr827646495chr827394301823GIKYVGVAEAELEQNGGAATCAAATATGTGGGAGTGGCTGAGGCTGAACTGGAACAGAAC

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Information of the samples that have these potential fusion neoantigens of ESCO2-EPHX2

check button These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens.
Cancer typeFusion geneHchrHbpHenstTchrTbpTenstSample
OVESCO2-EPHX2chr827646495ENST00000305188chr827394301ENST00000380476TCGA-23-1027-01A

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Potential target of CAR-T therapy development for ESCO2-EPHX2

check button Predicted 3D structure. We used RoseTTAFold.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features


* Minus value of BPloci means that the break point is located before the CDS.
- In-frame and retained 'Transmembrane'.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

check button Subcellular localization prediction of the transmembrane domain retained fusion proteins
* We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image.
HgeneHchrHbpHenstTgeneTchrTbpTenstDeepLoc result

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Related Drugs to ESCO2-EPHX2

check button Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDrugSourcePMID

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Related Diseases to ESCO2-EPHX2

check button Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDiseaseSourcePMID

check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource