|
|||||||
|
Fusion Protein:EYS-CAMSAP3 |
Fusion Gene and Fusion Protein Summary |
 Fusion gene summary |
| Fusion partner gene information | Fusion gene name: EYS-CAMSAP3 | FusionPDB ID: 28080 | FusionGDB2.0 ID: 28084 | Hgene | Tgene | Gene symbol | EYS | CAMSAP3 | Gene ID | 346007 | 57662 |
| Gene name | eyes shut homolog | calmodulin regulated spectrin associated protein family member 3 | |
| Synonyms | C6orf178|C6orf179|C6orf180|EGFL10|EGFL11|RP25|SPAM|bA166P24.2|bA307F22.3|bA74E24.1|dJ1018A4.2|dJ22I17.2|dJ303F19.1 | KIAA1543|NEZHA|PPP1R80 | |
| Cytomap | 6q12 | 19p13.2 | |
| Type of gene | protein-coding | protein-coding | |
| Description | protein eyes shut homologEGF-like-domain, multiple 10EGF-like-domain, multiple 11epidermal growth factor-like protein 10epidermal growth factor-like protein 11protein spacemaker homolog | calmodulin-regulated spectrin-associated protein 3protein phosphatase 1, regulatory subunit 80 | |
| Modification date | 20200313 | 20200313 | |
| UniProtAcc | Q5T1H1 Main function of 5'-partner protein: FUNCTION: Required to maintain the integrity of photoreceptor cells (PubMed:18836446). Specifically required for normal morphology of the photoreceptor ciliary pocket, and might thus facilitate protein trafficking between the photoreceptor inner and outer segments via the transition zone (By similarity). {ECO:0000250|UniProtKB:B8JI71, ECO:0000269|PubMed:18836446}. | Q9P1Y5 Main function of 5'-partner protein: FUNCTION: Key microtubule-organizing protein that specifically binds the minus-end of non-centrosomal microtubules and regulates their dynamics and organization (PubMed:19041755, PubMed:23169647). Specifically recognizes growing microtubule minus-ends and autonomously decorates and stabilizes microtubule lattice formed by microtubule minus-end polymerization (PubMed:24486153). Acts on free microtubule minus-ends that are not capped by microtubule-nucleating proteins or other factors and protects microtubule minus-ends from depolymerization (PubMed:24486153). In addition, it also reduces the velocity of microtubule polymerization (PubMed:24486153). Required for the biogenesis and the maintenance of zonula adherens by anchoring the minus-end of microtubules to zonula adherens and by recruiting the kinesin KIFC3 to those junctional sites (PubMed:19041755). Required for orienting the apical-to-basal polarity of microtubules in epithelial cells: acts by tethering non-centrosomal microtubules to the apical cortex, leading to their longitudinal orientation (PubMed:27802168, PubMed:26715742). Plays a key role in early embryos, which lack centrosomes: accumulates at the microtubule bridges that connect pairs of cells and enables the formation of a non-centrosomal microtubule-organizing center that directs intracellular transport in the early embryo (By similarity). Couples non-centrosomal microtubules with actin: interaction with MACF1 at the minus ends of non-centrosomal microtubules, tethers the microtubules to actin filaments, regulating focal adhesion size and cell migration (PubMed:27693509). Plays a key role in the generation of non-centrosomal microtubules by accumulating in the pericentrosomal region and cooperating with KATNA1 to release non-centrosomal microtubules from the centrosome (PubMed:28386021). Through the microtubule cytoskeleton, also regulates the organization of cellular organelles including the Golgi and the early endosomes (PubMed:28089391). Through interaction with AKAP9, involved in translocation of Golgi vesicles in epithelial cells, where microtubules are mainly non-centrosomal (PubMed:28089391). Plays an important role in motile cilia function by facilitatating proper orientation of basal bodies and formation of central microtubule pairs in motile cilia (By similarity). {ECO:0000250|UniProtKB:Q80VC9, ECO:0000269|PubMed:19041755, ECO:0000269|PubMed:23169647, ECO:0000269|PubMed:24486153, ECO:0000269|PubMed:26715742, ECO:0000269|PubMed:27693509, ECO:0000269|PubMed:27802168, ECO:0000269|PubMed:28089391, ECO:0000269|PubMed:28386021}. | |
| Ensembl transtripts involved in fusion gene | ENST ids | ENST00000370621, ENST00000503581, ENST00000370616, ENST00000342421, ENST00000370618, ENST00000393380, ENST00000486069, | ENST00000160298, ENST00000446248, |
| Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0) | * DoF score | 11 X 11 X 4=484 | 3 X 3 X 3=27 |
| # samples | 10 | 3 | |
| ** MAII score | log2(10/484*10)=-2.27500704749987 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | log2(3/27*10)=0.15200309344505 effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs). DoF>8 and MAII>0 | |
| Fusion gene context | PubMed: EYS [Title/Abstract] AND CAMSAP3 [Title/Abstract] AND fusion [Title/Abstract] | ||
| Fusion neoantigen context | PubMed: EYS [Title/Abstract] AND CAMSAP3 [Title/Abstract] AND neoantigen [Title/Abstract] | ||
| Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0) | EYS(65300116)-CAMSAP3(7670112), # samples:1 | ||
| Anticipated loss of major functional domain due to fusion event. | EYS-CAMSAP3 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. EYS-CAMSAP3 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. EYS-CAMSAP3 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. EYS-CAMSAP3 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. EYS-CAMSAP3 seems lost the major protein functional domain in Tgene partner, which is a essential gene due to the frame-shifted ORF. | ||
| * DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
| Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
| Partner | Gene | GO ID | GO term | PubMed ID |
| Tgene | CAMSAP3 | GO:0000226 | microtubule cytoskeleton organization | 24486153|27693509 |
| Tgene | CAMSAP3 | GO:0010923 | negative regulation of phosphatase activity | 19389623 |
| Tgene | CAMSAP3 | GO:0030334 | regulation of cell migration | 27693509 |
| Tgene | CAMSAP3 | GO:0030951 | establishment or maintenance of microtubule cytoskeleton polarity | 26715742|27802168 |
| Tgene | CAMSAP3 | GO:0031113 | regulation of microtubule polymerization | 24486153 |
| Tgene | CAMSAP3 | GO:0045198 | establishment of epithelial cell apical/basal polarity | 26715742|27802168 |
| Tgene | CAMSAP3 | GO:0051893 | regulation of focal adhesion assembly | 27693509 |
| Tgene | CAMSAP3 | GO:1903358 | regulation of Golgi organization | 28089391 |
| Four levels of functional features of fusion genes Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr6:65300116/chr19:7670112) - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. - How to search 1. Put your fusion gene symbol. 2. Press the tab key until there will be shown the breakpoint information filled. 4. Go down and press 'Search' tab twice. 4. Go down to have the hyperlink of the search result. 5. Click the hyperlink. 6. See the FGviewer result for your fusion gene. |
 |
| Retention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here. |
| Fusion gene breakpoints across EYS (5'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
 |
| Fusion gene breakpoints across CAMSAP3 (3'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
 |
Top |
Fusion Amino Acid Sequences |
| Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
| ENST00000370616 | EYS | chr6 | 65300116 | - | ENST00000160298 | CAMSAP3 | chr19 | 7670112 | + | 9284 | 5644 | 0 | 9245 | 3081 |
| ENST00000370616 | EYS | chr6 | 65300116 | - | ENST00000446248 | CAMSAP3 | chr19 | 7670112 | + | 9574 | 5644 | 0 | 9326 | 3108 |
| DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
| ENST00000370616 | ENST00000160298 | EYS | chr6 | 65300116 | - | CAMSAP3 | chr19 | 7670112 | + | 0.001044544 | 0.9989555 |
| ENST00000370616 | ENST00000446248 | EYS | chr6 | 65300116 | - | CAMSAP3 | chr19 | 7670112 | + | 0.000967962 | 0.999032 |
| Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones. |
Get the fusion protein sequences from here. |
| Fusion protein sequence information is available in the fasta format. >FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP |
Top |
Fusion Protein Breakpoint Sequences for EYS-CAMSAP3 |
| +/-13 AA sequence from the breakpoints of the fusion protein sequences. |
| Hgene | Hchr | Hbp | Tgene | Tchr | Tbp | Length(fusion protein) | BP in fusion protein | Peptide |
| EYS | chr6 | 65300116 | CAMSAP3 | chr19 | 7670112 | 5644 | 1881 | LESILAPQRLMISEHVPPELWEPFYT |
Top |
Potential FusionNeoAntigen Information of EYS-CAMSAP3 in HLA I |
| Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific. |
| EYS-CAMSAP3_65300116_7670112.msa |
| Potential FusionNeoAntigen Information * We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5) |
| Fusion gene | Hchr | Hbp | Tgene | Tchr | Tbp | HLA I | FusionNeoAntigen peptide | Binding score | Immunogenic score | Neoantigen start (at BP 13) | Neoantigen end (at BP 13) |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B50:02 | SEHVPPEL | 0.9997 | 0.56 | 12 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B47:01 | SEHVPPEL | 0.998 | 0.6557 | 12 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B45:01 | SEHVPPEL | 0.9963 | 0.8029 | 12 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B18:01 | SEHVPPEL | 0.9925 | 0.9553 | 12 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B41:01 | SEHVPPEL | 0.9767 | 0.7682 | 12 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B39:13 | SEHVPPEL | 0.9538 | 0.9457 | 12 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B50:01 | SEHVPPEL | 0.9417 | 0.7501 | 12 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B27:05 | QRLMISEHV | 0.9998 | 0.7103 | 7 | 16 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B39:06 | QRLMISEHV | 0.9993 | 0.7316 | 7 | 16 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B44:03 | SEHVPPELW | 0.9992 | 0.9791 | 12 | 21 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B18:01 | SEHVPPELW | 0.922 | 0.8764 | 12 | 21 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B13:01 | SEHVPPELW | 0.9146 | 0.982 | 12 | 21 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B47:01 | SEHVPPELW | 0.8985 | 0.7612 | 12 | 21 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B57:01 | ISEHVPPELW | 0.9999 | 0.9709 | 11 | 21 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B58:02 | ISEHVPPELW | 0.9994 | 0.9491 | 11 | 21 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B58:01 | ISEHVPPELW | 0.9991 | 0.9519 | 11 | 21 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B57:03 | ISEHVPPELW | 0.9983 | 0.9721 | 11 | 21 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B15:16 | ISEHVPPELW | 0.9938 | 0.7743 | 11 | 21 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B15:17 | ISEHVPPELW | 0.9885 | 0.9122 | 11 | 21 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B44:03 | ISEHVPPELW | 0.9737 | 0.9589 | 11 | 21 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B44:03 | SEHVPPELWE | 0.9384 | 0.9648 | 12 | 22 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B57:01 | MISEHVPPELW | 1 | 0.9875 | 10 | 21 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B58:02 | MISEHVPPELW | 0.9999 | 0.9841 | 10 | 21 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B58:01 | MISEHVPPELW | 0.9996 | 0.9765 | 10 | 21 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B57:03 | MISEHVPPELW | 0.9996 | 0.9904 | 10 | 21 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-A32:13 | MISEHVPPELW | 0.9993 | 0.9581 | 10 | 21 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B44:03 | MISEHVPPELW | 0.9947 | 0.9746 | 10 | 21 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B45:01 | SEHVPPELWEP | 0.9917 | 0.8115 | 12 | 23 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B40:06 | SEHVPPEL | 1 | 0.63 | 12 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B39:08 | SEHVPPEL | 0.9896 | 0.6991 | 12 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-C05:09 | ISEHVPPEL | 1 | 0.887 | 11 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-C04:07 | ISEHVPPEL | 1 | 0.6931 | 11 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-C08:15 | ISEHVPPEL | 1 | 0.9205 | 11 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-C04:10 | ISEHVPPEL | 1 | 0.6868 | 11 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B27:14 | QRLMISEHV | 0.9999 | 0.6428 | 7 | 16 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-C03:07 | ISEHVPPEL | 0.9994 | 0.9889 | 11 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-C03:19 | ISEHVPPEL | 0.9987 | 0.9937 | 11 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-C15:06 | ISEHVPPEL | 0.9986 | 0.951 | 11 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B73:01 | QRLMISEHV | 0.9984 | 0.5708 | 7 | 16 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-C03:08 | ISEHVPPEL | 0.9984 | 0.9499 | 11 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-C15:04 | ISEHVPPEL | 0.9983 | 0.9288 | 11 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-C04:06 | ISEHVPPEL | 0.9979 | 0.9108 | 11 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B40:03 | SEHVPPELW | 0.9915 | 0.6103 | 12 | 21 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-C08:04 | ISEHVPPEL | 0.9897 | 0.9656 | 11 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-C08:13 | ISEHVPPEL | 0.9897 | 0.9656 | 11 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B27:03 | QRLMISEHV | 0.9799 | 0.76 | 7 | 16 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-C04:14 | ISEHVPPEL | 0.9604 | 0.8038 | 11 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-C08:03 | ISEHVPPEL | 0.932 | 0.9785 | 11 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-C03:14 | ISEHVPPEL | 0.9142 | 0.9759 | 11 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B44:10 | SEHVPPELW | 0.8985 | 0.7072 | 12 | 21 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-C02:06 | ISEHVPPEL | 0.4533 | 0.9611 | 11 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B73:01 | QRLMISEHVP | 0.9938 | 0.5882 | 7 | 17 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B44:10 | ISEHVPPELW | 0.6005 | 0.547 | 11 | 21 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B40:04 | SEHVPPEL | 0.9999 | 0.7277 | 12 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B18:08 | SEHVPPEL | 0.9934 | 0.9265 | 12 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B18:06 | SEHVPPEL | 0.9931 | 0.9611 | 12 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B18:03 | SEHVPPEL | 0.9926 | 0.9514 | 12 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B18:05 | SEHVPPEL | 0.9925 | 0.9553 | 12 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B18:04 | SEHVPPEL | 0.9924 | 0.9589 | 12 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B39:11 | SEHVPPEL | 0.985 | 0.6474 | 12 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B18:11 | SEHVPPEL | 0.9808 | 0.9272 | 12 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B39:02 | SEHVPPEL | 0.9601 | 0.9483 | 12 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B39:31 | SEHVPPEL | 0.9579 | 0.955 | 12 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B50:04 | SEHVPPEL | 0.9417 | 0.7501 | 12 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B50:05 | SEHVPPEL | 0.9417 | 0.7501 | 12 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-C05:01 | ISEHVPPEL | 1 | 0.887 | 11 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-C18:01 | ISEHVPPEL | 1 | 0.6987 | 11 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-C04:01 | ISEHVPPEL | 1 | 0.6931 | 11 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-C04:03 | ISEHVPPEL | 1 | 0.734 | 11 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-C08:02 | ISEHVPPEL | 1 | 0.9205 | 11 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-C01:03 | ISEHVPPEL | 0.9999 | 0.8432 | 11 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B27:09 | QRLMISEHV | 0.9999 | 0.6939 | 7 | 16 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B27:08 | QRLMISEHV | 0.9998 | 0.5622 | 7 | 16 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B44:07 | SEHVPPELW | 0.9992 | 0.9791 | 12 | 21 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B44:13 | SEHVPPELW | 0.9992 | 0.9791 | 12 | 21 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B44:26 | SEHVPPELW | 0.9992 | 0.9791 | 12 | 21 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-C03:04 | ISEHVPPEL | 0.9988 | 0.9929 | 11 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-C03:03 | ISEHVPPEL | 0.9988 | 0.9929 | 11 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-C15:05 | ISEHVPPEL | 0.9986 | 0.9252 | 11 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-C15:09 | ISEHVPPEL | 0.9983 | 0.9288 | 11 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-C15:02 | ISEHVPPEL | 0.9982 | 0.9182 | 11 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-C03:17 | ISEHVPPEL | 0.9978 | 0.9781 | 11 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-C03:05 | ISEHVPPEL | 0.9968 | 0.9574 | 11 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-C03:06 | ISEHVPPEL | 0.9822 | 0.9933 | 11 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-C16:02 | ISEHVPPEL | 0.941 | 0.9891 | 11 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-C16:01 | ISEHVPPEL | 0.9371 | 0.9731 | 11 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-C08:01 | ISEHVPPEL | 0.932 | 0.9785 | 11 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B18:05 | SEHVPPELW | 0.922 | 0.8764 | 12 | 21 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B18:08 | SEHVPPELW | 0.9219 | 0.9776 | 12 | 21 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B18:06 | SEHVPPELW | 0.881 | 0.9129 | 12 | 21 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B18:03 | SEHVPPELW | 0.8351 | 0.8701 | 12 | 21 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B18:11 | SEHVPPELW | 0.7814 | 0.8856 | 12 | 21 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-C17:01 | ISEHVPPEL | 0.7796 | 0.8869 | 11 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B35:13 | ISEHVPPEL | 0.7316 | 0.9507 | 11 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B57:02 | ISEHVPPEL | 0.6897 | 0.7454 | 11 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B15:13 | SEHVPPELW | 0.3506 | 0.9262 | 12 | 21 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B15:24 | SEHVPPELW | 0.1728 | 0.9727 | 12 | 21 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B48:02 | SEHVPPELW | 0.0858 | 0.8731 | 12 | 21 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B07:13 | ISEHVPPEL | 0.016 | 0.7459 | 11 | 20 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B57:10 | ISEHVPPELW | 0.9999 | 0.9709 | 11 | 21 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B57:04 | ISEHVPPELW | 0.9995 | 0.7295 | 11 | 21 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B58:06 | ISEHVPPELW | 0.9984 | 0.9146 | 11 | 21 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B57:02 | ISEHVPPELW | 0.9962 | 0.847 | 11 | 21 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B44:07 | ISEHVPPELW | 0.9737 | 0.9589 | 11 | 21 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B44:26 | ISEHVPPELW | 0.9737 | 0.9589 | 11 | 21 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B44:13 | ISEHVPPELW | 0.9737 | 0.9589 | 11 | 21 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B44:07 | SEHVPPELWE | 0.9384 | 0.9648 | 12 | 22 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B44:13 | SEHVPPELWE | 0.9384 | 0.9648 | 12 | 22 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B44:26 | SEHVPPELWE | 0.9384 | 0.9648 | 12 | 22 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B57:10 | MISEHVPPELW | 1 | 0.9875 | 10 | 21 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B57:04 | MISEHVPPELW | 0.9999 | 0.7824 | 10 | 21 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B57:02 | MISEHVPPELW | 0.9996 | 0.9327 | 10 | 21 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B44:07 | MISEHVPPELW | 0.9947 | 0.9746 | 10 | 21 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B44:13 | MISEHVPPELW | 0.9947 | 0.9746 | 10 | 21 |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 | HLA-B44:26 | MISEHVPPELW | 0.9947 | 0.9746 | 10 | 21 |
Top |
Potential FusionNeoAntigen Information of EYS-CAMSAP3 in HLA II |
| Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific. |
| Potential FusionNeoAntigen Information * We used NetMHCIIpan v4.1 (%rank<0.5). |
| Fusion gene | Hchr | Hbp | Tgene | Tchr | Tbp | HLA II | FusionNeoAntigen peptide | Neoantigen start (at BP 13) | Neoantigen end (at BP 13) |
Top |
Fusion breakpoint peptide structures of EYS-CAMSAP3 |
| 3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens * The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA. |
| File name | BPseq | Hgene | Tgene | Hchr | Hbp | Tchr | Tbp | AAlen |
| 6925 | PQRLMISEHVPPEL | EYS | CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 5644 |
Top |
Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of EYS-CAMSAP3 |
| Virtual screening between 25 HLAs (from PDB) and FusionNeoAntigens * We used Glide to predict the interaction between HLAs and neoantigens. |
| HLA allele | PDB ID | File name | BPseq | Docking score | Glide score |
| HLA-B14:02 | 3BVN | 6925 | PQRLMISEHVPPEL | -7.15543 | -7.26883 |
| HLA-B14:02 | 3BVN | 6925 | PQRLMISEHVPPEL | -4.77435 | -5.80965 |
| HLA-B52:01 | 3W39 | 6925 | PQRLMISEHVPPEL | -6.80875 | -6.92215 |
| HLA-B52:01 | 3W39 | 6925 | PQRLMISEHVPPEL | -4.20386 | -5.23916 |
| HLA-A11:01 | 4UQ2 | 6925 | PQRLMISEHVPPEL | -7.5194 | -8.5547 |
| HLA-A11:01 | 4UQ2 | 6925 | PQRLMISEHVPPEL | -6.9601 | -7.0735 |
| HLA-A24:02 | 5HGA | 6925 | PQRLMISEHVPPEL | -7.52403 | -7.63743 |
| HLA-A24:02 | 5HGA | 6925 | PQRLMISEHVPPEL | -5.82433 | -6.85963 |
| HLA-B27:05 | 6PYJ | 6925 | PQRLMISEHVPPEL | -3.28285 | -4.31815 |
| HLA-B44:05 | 3DX8 | 6925 | PQRLMISEHVPPEL | -5.91172 | -6.94702 |
| HLA-B44:05 | 3DX8 | 6925 | PQRLMISEHVPPEL | -4.24346 | -4.35686 |
Top |
Vaccine Design for the FusionNeoAntigens of EYS-CAMSAP3 |
| mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is. |
| Fusion gene | Hchr | Hbp | Tchr | Tbp | Start in +/-13AA | End in +/-13AA | FusionNeoAntigen peptide sequence | FusionNeoAntigen RNA sequence |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 10 | 21 | MISEHVPPELW | TGATTTCTGAGCACGTGCCCCCGGAGCTGTGGG |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 11 | 20 | ISEHVPPEL | TTTCTGAGCACGTGCCCCCGGAGCTGT |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 11 | 21 | ISEHVPPELW | TTTCTGAGCACGTGCCCCCGGAGCTGTGGG |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 12 | 20 | SEHVPPEL | CTGAGCACGTGCCCCCGGAGCTGT |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 12 | 21 | SEHVPPELW | CTGAGCACGTGCCCCCGGAGCTGTGGG |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 12 | 22 | SEHVPPELWE | CTGAGCACGTGCCCCCGGAGCTGTGGGAGC |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 12 | 23 | SEHVPPELWEP | CTGAGCACGTGCCCCCGGAGCTGTGGGAGCCCT |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 7 | 16 | QRLMISEHV | AACGGCTGATGATTTCTGAGCACGTGC |
| EYS-CAMSAP3 | chr6 | 65300116 | chr19 | 7670112 | 7 | 17 | QRLMISEHVP | AACGGCTGATGATTTCTGAGCACGTGCCCC |
| mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs. |
| Fusion gene | Hchr | Hbp | Tchr | Tbp | Start in +/-13AA | End in +/-13AA | FusionNeoAntigen peptide | FusionNEoAntigen RNA sequence |
Top |
Information of the samples that have these potential fusion neoantigens of EYS-CAMSAP3 |
| These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens. |
| Cancer type | Fusion gene | Hchr | Hbp | Henst | Tchr | Tbp | Tenst | Sample |
| LGG | EYS-CAMSAP3 | chr6 | 65300116 | ENST00000370616 | chr19 | 7670112 | ENST00000160298 | TCGA-TM-A7CF |
Top |
Potential target of CAR-T therapy development for EYS-CAMSAP3 |
| Predicted 3D structure. We used RoseTTAFold. |
| Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features * Minus value of BPloci means that the break point is located before the CDS. |
| - In-frame and retained 'Transmembrane'. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| Subcellular localization prediction of the transmembrane domain retained fusion proteins * We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image. |
| Hgene | Hchr | Hbp | Henst | Tgene | Tchr | Tbp | Tenst | DeepLoc result |
Top |
Related Drugs to EYS-CAMSAP3 |
| Drugs used for this fusion-positive patient. (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
| Hgene | Tgene | Drug | Source | PMID |
Top |
Related Diseases to EYS-CAMSAP3 |
| Diseases that have this fusion gene. (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
| Hgene | Tgene | Disease | Source | PMID |
| Diseases associated with fusion partners. (DisGeNet 4.0) |
| Partner | Gene | Disease ID | Disease name | # pubmeds | Source |
Copyright 2023-PresentThe University of Texas Health Science Center at Houston (UTHealth)
Web File Viewing | Emergency Information
Campus Carry|Site Policies