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Fusion Protein:FBN1-FUS |
Fusion Gene and Fusion Protein Summary |
 Fusion gene summary |
| Fusion partner gene information | Fusion gene name: FBN1-FUS | FusionPDB ID: 29559 | FusionGDB2.0 ID: 29559 | Hgene | Tgene | Gene symbol | FBN1 | FUS | Gene ID | 2200 | 2521 |
| Gene name | fibrillin 1 | FUS RNA binding protein | |
| Synonyms | ACMICD|ECTOL1|FBN|GPHYSD2|MASS|MFLS|MFS1|OCTD|SGS|SSKS|WMS|WMS2 | ALS6|ETM4|FUS1|HNRNPP2|POMP75|TLS | |
| Cytomap | 15q21.1 | 16p11.2 | |
| Type of gene | protein-coding | protein-coding | |
| Description | fibrillin-1asprosinepididymis secretory sperm binding proteinfibrillin 15fibrillin-1 preproprotein | RNA-binding protein FUS75 kDa DNA-pairing proteinfus-like proteinfused in sarcomafusion gene in myxoid liposarcomaheterogeneous nuclear ribonucleoprotein P2oncogene FUSoncogene TLStranslocated in liposarcoma protein | |
| Modification date | 20200313 | 20200329 | |
| UniProtAcc | P35555 Main function of 5'-partner protein: FUNCTION: [Fibrillin-1]: Structural component of the 10-12 nm diameter microfibrils of the extracellular matrix, which conveys both structural and regulatory properties to load-bearing connective tissues (PubMed:1860873, PubMed:15062093). Fibrillin-1-containing microfibrils provide long-term force bearing structural support. In tissues such as the lung, blood vessels and skin, microfibrils form the periphery of the elastic fiber, acting as a scaffold for the deposition of elastin. In addition, microfibrils can occur as elastin-independent networks in tissues such as the ciliary zonule, tendon, cornea and glomerulus where they provide tensile strength and have anchoring roles. Fibrillin-1 also plays a key role in tissue homeostasis through specific interactions with growth factors, such as the bone morphogenetic proteins (BMPs), growth and differentiation factors (GDFs) and latent transforming growth factor-beta-binding proteins (LTBPs), cell-surface integrins and other extracellular matrix protein and proteoglycan components (PubMed:27026396). Regulates osteoblast maturation by controlling TGF-beta bioavailability and calibrating TGF-beta and BMP levels, respectively (By similarity). Negatively regulates osteoclastogenesis by binding and sequestering an osteoclast differentiation and activation factor TNFSF11. This leads to disruption of TNFSF11-induced Ca(2+) signaling and impairment of TNFSF11-mediated nuclear translocation and activation of transcription factor NFATC1 which regulates genes important for osteoclast differentiation and function (PubMed:24039232). Mediates cell adhesion via its binding to cell surface receptors integrins ITGAV:ITGB3 and ITGA5:ITGB1 (PubMed:12807887, PubMed:17158881). Binds heparin and this interaction has an important role in the assembly of microfibrils (PubMed:11461921). {ECO:0000250|UniProtKB:Q61554, ECO:0000269|PubMed:11461921, ECO:0000269|PubMed:12807887, ECO:0000269|PubMed:15062093, ECO:0000269|PubMed:17158881, ECO:0000269|PubMed:1860873, ECO:0000269|PubMed:24039232, ECO:0000303|PubMed:27026396}.; FUNCTION: [Asprosin]: Hormone that targets the liver to increase plasma glucose levels. Secreted by white adipose tissue and circulates in the plasma. Acts in response to fasting and promotes blood glucose elevation by binding to the surface of hepatocytes. Promotes hepatocyte glucose release by activating the protein kinase A activity in the liver, resulting in rapid glucose release into the circulation. {ECO:0000269|PubMed:27087445}. | P35637 Main function of 5'-partner protein: FUNCTION: DNA/RNA-binding protein that plays a role in various cellular processes such as transcription regulation, RNA splicing, RNA transport, DNA repair and damage response (PubMed:27731383). Binds to nascent pre-mRNAs and acts as a molecular mediator between RNA polymerase II and U1 small nuclear ribonucleoprotein thereby coupling transcription and splicing (PubMed:26124092). Binds also its own pre-mRNA and autoregulates its expression; this autoregulation mechanism is mediated by non-sense-mediated decay (PubMed:24204307). Plays a role in DNA repair mechanisms by promoting D-loop formation and homologous recombination during DNA double-strand break repair (PubMed:10567410). In neuronal cells, plays crucial roles in dendritic spine formation and stability, RNA transport, mRNA stability and synaptic homeostasis (By similarity). {ECO:0000250|UniProtKB:P56959, ECO:0000269|PubMed:10567410, ECO:0000269|PubMed:24204307, ECO:0000269|PubMed:26124092, ECO:0000269|PubMed:27731383}. | |
| Ensembl transtripts involved in fusion gene | ENST ids | ENST00000316623, ENST00000560355, ENST00000561429, | ENST00000474990, ENST00000254108, ENST00000380244, ENST00000568685, |
| Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0) | * DoF score | 15 X 17 X 7=1785 | 20 X 13 X 10=2600 |
| # samples | 17 | 22 | |
| ** MAII score | log2(17/1785*10)=-3.39231742277876 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | log2(22/2600*10)=-3.56293619439116 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | |
| Fusion gene context | PubMed: FBN1 [Title/Abstract] AND FUS [Title/Abstract] AND fusion [Title/Abstract] | ||
| Fusion neoantigen context | PubMed: FBN1 [Title/Abstract] AND FUS [Title/Abstract] AND neoantigen [Title/Abstract] | ||
| Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0) | FBN1(48786401)-FUS(31199646), # samples:1 | ||
| Anticipated loss of major functional domain due to fusion event. | FBN1-FUS seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. FBN1-FUS seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. FBN1-FUS seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. FBN1-FUS seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. | ||
| * DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
| Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
| Partner | Gene | GO ID | GO term | PubMed ID |
| Hgene | FBN1 | GO:0033627 | cell adhesion mediated by integrin | 12807887|17158881 |
| Hgene | FBN1 | GO:0045671 | negative regulation of osteoclast differentiation | 24039232 |
| Hgene | FBN1 | GO:2001205 | negative regulation of osteoclast development | 24039232 |
| Tgene | FUS | GO:0006355 | regulation of transcription, DNA-templated | 26124092 |
| Tgene | FUS | GO:0006357 | regulation of transcription by RNA polymerase II | 25453086 |
| Tgene | FUS | GO:0008380 | RNA splicing | 26124092 |
| Tgene | FUS | GO:0043484 | regulation of RNA splicing | 25453086|27731383 |
| Tgene | FUS | GO:0048255 | mRNA stabilization | 27378374 |
| Tgene | FUS | GO:0051260 | protein homooligomerization | 25453086 |
| Tgene | FUS | GO:1905168 | positive regulation of double-strand break repair via homologous recombination | 10567410 |
| Four levels of functional features of fusion genes Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr15:48786401/chr16:31199646) - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. - How to search 1. Put your fusion gene symbol. 2. Press the tab key until there will be shown the breakpoint information filled. 4. Go down and press 'Search' tab twice. 4. Go down to have the hyperlink of the search result. 5. Click the hyperlink. 6. See the FGviewer result for your fusion gene. |
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| Retention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here. |
| Fusion gene breakpoints across FBN1 (5'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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| Fusion gene breakpoints across FUS (3'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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Fusion Amino Acid Sequences |
| Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
| ENST00000316623 | FBN1 | chr15 | 48786401 | - | ENST00000254108 | FUS | chr16 | 31199646 | + | 4334 | 3184 | 84 | 3965 | 1293 |
| ENST00000316623 | FBN1 | chr15 | 48786401 | - | ENST00000380244 | FUS | chr16 | 31199646 | + | 4130 | 3184 | 84 | 3965 | 1293 |
| ENST00000316623 | FBN1 | chr15 | 48786401 | - | ENST00000568685 | FUS | chr16 | 31199646 | + | 4106 | 3184 | 84 | 3968 | 1294 |
| DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
| ENST00000316623 | ENST00000254108 | FBN1 | chr15 | 48786401 | - | FUS | chr16 | 31199646 | + | 0.000492639 | 0.9995073 |
| ENST00000316623 | ENST00000380244 | FBN1 | chr15 | 48786401 | - | FUS | chr16 | 31199646 | + | 0.000514298 | 0.99948573 |
| ENST00000316623 | ENST00000568685 | FBN1 | chr15 | 48786401 | - | FUS | chr16 | 31199646 | + | 0.000426996 | 0.99957305 |
| Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones. |
Get the fusion protein sequences from here. |
| Fusion protein sequence information is available in the fasta format. >FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP |
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Fusion Protein Breakpoint Sequences for FBN1-FUS |
| +/-13 AA sequence from the breakpoints of the fusion protein sequences. |
| Hgene | Hchr | Hbp | Tgene | Tchr | Tbp | Length(fusion protein) | BP in fusion protein | Peptide |
| FBN1 | chr15 | 48786401 | FUS | chr16 | 31199646 | 3184 | 101 | GIAGPRRRGALQGAAAAADRAPGAAR |
| FBN1 | chr15 | 48786401 | FUS | chr16 | 31199646 | 3184 | 1148 | ATRRADFNRGGGNGRGGRGRGGPMGR |
| FBN1 | chr15 | 48786401 | FUS | chr16 | 31199646 | 3184 | 1149 | ATRRADFNRGGGNGRGGRGRGGPMGR |
| FBN1 | chr15 | 48786401 | FUS | chr16 | 31199646 | 3184 | 1149 | TRRADFNRGGGNGRGGRGRGGPMGRG |
| FBN1 | chr15 | 48786401 | FUS | chr16 | 31199646 | 3184 | 1150 | TRRADFNRGGGNGRGGRGRGGPMGRG |
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Potential FusionNeoAntigen Information of FBN1-FUS in HLA I |
| Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific. |
| Potential FusionNeoAntigen Information * We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5) |
| Fusion gene | Hchr | Hbp | Tgene | Tchr | Tbp | HLA I | FusionNeoAntigen peptide | Binding score | Immunogenic score | Neoantigen start (at BP 13) | Neoantigen end (at BP 13) |
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Potential FusionNeoAntigen Information of FBN1-FUS in HLA II |
| Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific. |
| Potential FusionNeoAntigen Information * We used NetMHCIIpan v4.1 (%rank<0.5). |
| Fusion gene | Hchr | Hbp | Tgene | Tchr | Tbp | HLA II | FusionNeoAntigen peptide | Neoantigen start (at BP 13) | Neoantigen end (at BP 13) |
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Fusion breakpoint peptide structures of FBN1-FUS |
| 3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens * The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA. |
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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of FBN1-FUS |
| Virtual screening between 25 HLAs (from PDB) and FusionNeoAntigens * We used Glide to predict the interaction between HLAs and neoantigens. |
| HLA allele | PDB ID | File name | BPseq | Docking score | Glide score |
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Vaccine Design for the FusionNeoAntigens of FBN1-FUS |
| mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is. |
| Fusion gene | Hchr | Hbp | Tchr | Tbp | Start in +/-13AA | End in +/-13AA | FusionNeoAntigen peptide sequence | FusionNeoAntigen RNA sequence |
| mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs. |
| Fusion gene | Hchr | Hbp | Tchr | Tbp | Start in +/-13AA | End in +/-13AA | FusionNeoAntigen peptide | FusionNEoAntigen RNA sequence |
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Information of the samples that have these potential fusion neoantigens of FBN1-FUS |
| These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens. |
| Cancer type | Fusion gene | Hchr | Hbp | Henst | Tchr | Tbp | Tenst | Sample |
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Potential target of CAR-T therapy development for FBN1-FUS |
| Predicted 3D structure. We used RoseTTAFold. |
| Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features * Minus value of BPloci means that the break point is located before the CDS. |
| - In-frame and retained 'Transmembrane'. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| Subcellular localization prediction of the transmembrane domain retained fusion proteins * We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image. |
| Hgene | Hchr | Hbp | Henst | Tgene | Tchr | Tbp | Tenst | DeepLoc result |
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Related Drugs to FBN1-FUS |
| Drugs used for this fusion-positive patient. (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
| Hgene | Tgene | Drug | Source | PMID |
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Related Diseases to FBN1-FUS |
| Diseases that have this fusion gene. (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
| Hgene | Tgene | Disease | Source | PMID |
| Diseases associated with fusion partners. (DisGeNet 4.0) |
| Partner | Gene | Disease ID | Disease name | # pubmeds | Source |
| Tgene | FUS | C1842675 | AMYOTROPHIC LATERAL SCLEROSIS 6 (disorder) | 5 | UNIPROT |
| Tgene | FUS | C3468114 | Juvenile amyotrophic lateral sclerosis | 5 | ORPHANET |
| Tgene | FUS | C0002736 | Amyotrophic Lateral Sclerosis | 2 | CTD_human;ORPHANET |
| Tgene | FUS | C0206634 | Liposarcoma, Myxoid | 2 | CTD_human;ORPHANET |
| Tgene | FUS | C0393554 | Amyotrophic Lateral Sclerosis With Dementia | 1 | CTD_human |
| Tgene | FUS | C0497327 | Dementia | 1 | GENOMICS_ENGLAND |
| Tgene | FUS | C0543859 | Amyotrophic Lateral Sclerosis, Guam Form | 1 | CTD_human |
| Tgene | FUS | C3539195 | TREMOR, HEREDITARY ESSENTIAL, 4 | 1 | CTD_human;UNIPROT |
| Tgene | FUS | C3888102 | Frontotemporal Dementia With Motor Neuron Disease | 1 | ORPHANET |
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