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Center for Computational Systems Medicine
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Fusion Gene and Fusion Protein Summary

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Fusion Amino Acid Sequences (multiple BPs and multiple gene isoforms)

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Fusion Protein Breakpoint Sequences - (for the Screening of the FusionNeoAntigens)

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Potential FusionNeoAntigens in HLA I - (netMHCpan v4.1 + deepHLApan v1.1)

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Potential FusionNeoAntigens in HLA II - (netMHCIIpan v4.1)

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Fusion Breakpoint 14 AA Peptide Structure - (RoseTTAFold)

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D - (Glide)

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Vaccine Design for the FusionNeoAntigens (RNA/protein sequences)

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Potential target of CAR-T therapy development

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Information on the samples that have these potential fusion neoantigens

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Fusion Protein Targeting Drugs - (Manual Curation)

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Fusion Protein Related diseases - (Manual Curation)

Fusion Protein:GNAS-MACF1

Fusion Gene and Fusion Protein Summary

check button Fusion gene summary
Fusion partner gene informationFusion gene name: GNAS-MACF1
FusionPDB ID: 33635
FusionGDB2.0 ID: 33635
HgeneTgene
Gene symbol

GNAS

MACF1

Gene ID

2778

23499

Gene nameGNAS complex locusmicrotubule actin crosslinking factor 1
SynonymsAHO|C20orf45|GNAS1|GPSA|GSA|GSP|NESP|PITA3|POH|SCG6|SgVIABP620|ACF7|LIS9|MACF|OFC4
Cytomap

20q13.32

1p34.3

Type of geneprotein-codingprotein-coding
Descriptionprotein ALEXprotein GNASprotein SCG6 (secretogranin VI)G protein subunit alpha Sadenylate cyclase-stimulating G alpha proteinalternative gene product encoded by XL-exonextra large alphas proteinguanine nucleotide binding protein (G protein), alpha microtubule-actin cross-linking factor 1620 kDa actin binding proteinactin cross-linking family protein 7macrophin 1 isoformtrabeculin-alpha
Modification date2020032920200313
UniProtAcc

P63092

Main function of 5'-partner protein: FUNCTION: Guanine nucleotide-binding proteins (G proteins) function as transducers in numerous signaling pathways controlled by G protein-coupled receptors (GPCRs) (PubMed:17110384). Signaling involves the activation of adenylyl cyclases, resulting in increased levels of the signaling molecule cAMP (PubMed:26206488, PubMed:8702665). GNAS functions downstream of several GPCRs, including beta-adrenergic receptors (PubMed:21488135). Stimulates the Ras signaling pathway via RAPGEF2 (PubMed:12391161). {ECO:0000269|PubMed:12391161, ECO:0000269|PubMed:17110384, ECO:0000269|PubMed:21488135, ECO:0000269|PubMed:26206488, ECO:0000269|PubMed:8702665}.

Q9UPN3

Main function of 5'-partner protein: FUNCTION: [Isoform 2]: F-actin-binding protein which plays a role in cross-linking actin to other cytoskeletal proteins and also binds to microtubules (PubMed:15265687, PubMed:20937854). Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex (PubMed:20937854). Acts as a positive regulator of Wnt receptor signaling pathway and is involved in the translocation of AXIN1 and its associated complex (composed of APC, CTNNB1 and GSK3B) from the cytoplasm to the cell membrane (By similarity). Has actin-regulated ATPase activity and is essential for controlling focal adhesions (FAs) assembly and dynamics (By similarity). Interaction with CAMSAP3 at the minus ends of non-centrosomal microtubules tethers microtubules minus-ends to actin filaments, regulating focal adhesion size and cell migration (PubMed:27693509). May play role in delivery of transport vesicles containing GPI-linked proteins from the trans-Golgi network through its interaction with GOLGA4 (PubMed:15265687). Plays a key role in wound healing and epidermal cell migration (By similarity). Required for efficient upward migration of bulge cells in response to wounding and this function is primarily rooted in its ability to coordinate microtubule dynamics and polarize hair follicle stem cells (By similarity). As a regulator of actin and microtubule arrangement and stabilization, it plays an essential role in neurite outgrowth, branching and spine formation during brain development (By similarity). {ECO:0000250|UniProtKB:Q9QXZ0, ECO:0000269|PubMed:15265687, ECO:0000269|PubMed:20937854, ECO:0000269|PubMed:27693509}.
Ensembl transtripts involved in fusion geneENST idsENST00000265620, ENST00000306090, 
ENST00000313949, ENST00000354359, 
ENST00000371075, ENST00000371085, 
ENST00000371095, ENST00000371100, 
ENST00000371102, ENST00000464624, 
ENST00000306120, ENST00000371081, 
ENST00000371098, ENST00000371099, 
ENST00000476350, ENST00000484793, 
ENST00000536367, ENST00000602421, 
ENST00000289893, ENST00000317713, 
ENST00000361689, ENST00000372915, 
ENST00000539005, ENST00000545844, 
ENST00000564288, ENST00000567887, 
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)* DoF score44 X 25 X 16=1760024 X 23 X 9=4968
# samples 5124
** MAII scorelog2(51/17600*10)=-5.10893437155316
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(24/4968*10)=-4.37155886261196
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Fusion gene context

PubMed: GNAS [Title/Abstract] AND MACF1 [Title/Abstract] AND fusion [Title/Abstract]

Fusion neoantigen context

PubMed: GNAS [Title/Abstract] AND MACF1 [Title/Abstract] AND neoantigen [Title/Abstract]

Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)GNAS(57486244)-MACF1(39900083), # samples:1
Anticipated loss of major functional domain due to fusion event.GNAS-MACF1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
GNAS-MACF1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
GNAS-MACF1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
GNAS-MACF1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
GNAS-MACF1 seems lost the major protein functional domain in Hgene partner, which is a cell metabolism gene due to the frame-shifted ORF.
GNAS-MACF1 seems lost the major protein functional domain in Hgene partner, which is a CGC due to the frame-shifted ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
TgeneMACF1

GO:0030334

regulation of cell migration

27693509

TgeneMACF1

GO:0043001

Golgi to plasma membrane protein transport

15265687

TgeneMACF1

GO:0045773

positive regulation of axon extension

22705394

TgeneMACF1

GO:0051893

regulation of focal adhesion assembly

27693509



check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr20:57486244/chr1:39900083)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonRetention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here.

check buttonFusion gene breakpoints across GNAS (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check buttonFusion gene breakpoints across MACF1 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure


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Fusion Amino Acid Sequences


check buttonFusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000371100GNASchr2057486244+ENST00000545844MACF1chr139900083-102664029424289421566
ENST00000371100GNASchr2057486244+ENST00000567887MACF1chr139900083-105154029424291311629
ENST00000371100GNASchr2057486244+ENST00000372915MACF1chr139900083-101294029424289421566
ENST00000371100GNASchr2057486244+ENST00000361689MACF1chr139900083-101364029424289421566
ENST00000371100GNASchr2057486244+ENST00000539005MACF1chr139900083-102664029424289421566
ENST00000371100GNASchr2057486244+ENST00000317713MACF1chr139900083-102664029424289421566
ENST00000371100GNASchr2057486244+ENST00000564288MACF1chr139900083-105154029424291311629
ENST00000371100GNASchr2057486244+ENST00000289893MACF1chr139900083-101544029424289601572
ENST00000371102GNASchr2057486244+ENST00000545844MACF1chr139900083-96753438365183511566
ENST00000371102GNASchr2057486244+ENST00000567887MACF1chr139900083-99243438365185401629
ENST00000371102GNASchr2057486244+ENST00000372915MACF1chr139900083-95383438365183511566
ENST00000371102GNASchr2057486244+ENST00000361689MACF1chr139900083-95453438365183511566
ENST00000371102GNASchr2057486244+ENST00000539005MACF1chr139900083-96753438365183511566
ENST00000371102GNASchr2057486244+ENST00000317713MACF1chr139900083-96753438365183511566
ENST00000371102GNASchr2057486244+ENST00000564288MACF1chr139900083-99243438365185401629
ENST00000371102GNASchr2057486244+ENST00000289893MACF1chr139900083-95633438365183691572
ENST00000371095GNASchr2057486244+ENST00000545844MACF1chr139900083-81681931214468441566
ENST00000371095GNASchr2057486244+ENST00000567887MACF1chr139900083-84171931214470331629
ENST00000371095GNASchr2057486244+ENST00000372915MACF1chr139900083-80311931214468441566
ENST00000371095GNASchr2057486244+ENST00000361689MACF1chr139900083-80381931214468441566
ENST00000371095GNASchr2057486244+ENST00000539005MACF1chr139900083-81681931214468441566
ENST00000371095GNASchr2057486244+ENST00000317713MACF1chr139900083-81681931214468441566
ENST00000371095GNASchr2057486244+ENST00000564288MACF1chr139900083-84171931214470331629
ENST00000371095GNASchr2057486244+ENST00000289893MACF1chr139900083-80561931214468621572
ENST00000371085GNASchr2057486244+ENST00000545844MACF1chr139900083-82091972218568851566
ENST00000371085GNASchr2057486244+ENST00000567887MACF1chr139900083-84581972218570741629
ENST00000371085GNASchr2057486244+ENST00000372915MACF1chr139900083-80721972218568851566
ENST00000371085GNASchr2057486244+ENST00000361689MACF1chr139900083-80791972218568851566
ENST00000371085GNASchr2057486244+ENST00000539005MACF1chr139900083-82091972218568851566
ENST00000371085GNASchr2057486244+ENST00000317713MACF1chr139900083-82091972218568851566
ENST00000371085GNASchr2057486244+ENST00000564288MACF1chr139900083-84581972218570741629
ENST00000371085GNASchr2057486244+ENST00000289893MACF1chr139900083-80971972218569031572
ENST00000354359GNASchr2057486244+ENST00000545844MACF1chr139900083-82121975218868881566
ENST00000354359GNASchr2057486244+ENST00000567887MACF1chr139900083-84611975218870771629
ENST00000354359GNASchr2057486244+ENST00000372915MACF1chr139900083-80751975218868881566
ENST00000354359GNASchr2057486244+ENST00000361689MACF1chr139900083-80821975218868881566
ENST00000354359GNASchr2057486244+ENST00000539005MACF1chr139900083-82121975218868881566
ENST00000354359GNASchr2057486244+ENST00000317713MACF1chr139900083-82121975218868881566
ENST00000354359GNASchr2057486244+ENST00000564288MACF1chr139900083-84611975218870771629
ENST00000354359GNASchr2057486244+ENST00000289893MACF1chr139900083-81001975218869061572
ENST00000265620GNASchr2057486244+ENST00000545844MACF1chr139900083-81031866207967791566
ENST00000265620GNASchr2057486244+ENST00000567887MACF1chr139900083-83521866207969681629
ENST00000265620GNASchr2057486244+ENST00000372915MACF1chr139900083-79661866207967791566
ENST00000265620GNASchr2057486244+ENST00000361689MACF1chr139900083-79731866207967791566
ENST00000265620GNASchr2057486244+ENST00000539005MACF1chr139900083-81031866207967791566
ENST00000265620GNASchr2057486244+ENST00000317713MACF1chr139900083-81031866207967791566
ENST00000265620GNASchr2057486244+ENST00000564288MACF1chr139900083-83521866207969681629
ENST00000265620GNASchr2057486244+ENST00000289893MACF1chr139900083-79911866207967971572
ENST00000306090GNASchr2057486244+ENST00000545844MACF1chr139900083-78791642185565551566
ENST00000306090GNASchr2057486244+ENST00000567887MACF1chr139900083-81281642185567441629
ENST00000306090GNASchr2057486244+ENST00000372915MACF1chr139900083-77421642185565551566
ENST00000306090GNASchr2057486244+ENST00000361689MACF1chr139900083-77491642185565551566
ENST00000306090GNASchr2057486244+ENST00000539005MACF1chr139900083-78791642185565551566
ENST00000306090GNASchr2057486244+ENST00000317713MACF1chr139900083-78791642185565551566
ENST00000306090GNASchr2057486244+ENST00000564288MACF1chr139900083-81281642185567441629
ENST00000306090GNASchr2057486244+ENST00000289893MACF1chr139900083-77671642185565731572

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000371100ENST00000545844GNASchr2057486244+MACF1chr139900083-0.0252131580.9747868
ENST00000371100ENST00000567887GNASchr2057486244+MACF1chr139900083-0.027448660.9725513
ENST00000371100ENST00000372915GNASchr2057486244+MACF1chr139900083-0.025785260.9742148
ENST00000371100ENST00000361689GNASchr2057486244+MACF1chr139900083-0.0256756120.97432446
ENST00000371100ENST00000539005GNASchr2057486244+MACF1chr139900083-0.025109980.97489005
ENST00000371100ENST00000317713GNASchr2057486244+MACF1chr139900083-0.025109980.97489005
ENST00000371100ENST00000564288GNASchr2057486244+MACF1chr139900083-0.027448660.9725513
ENST00000371100ENST00000289893GNASchr2057486244+MACF1chr139900083-0.0242233970.9757766
ENST00000371102ENST00000545844GNASchr2057486244+MACF1chr139900083-0.0228794140.97712064
ENST00000371102ENST00000567887GNASchr2057486244+MACF1chr139900083-0.0251595340.9748405
ENST00000371102ENST00000372915GNASchr2057486244+MACF1chr139900083-0.023456180.9765438
ENST00000371102ENST00000361689GNASchr2057486244+MACF1chr139900083-0.0233481380.97665185
ENST00000371102ENST00000539005GNASchr2057486244+MACF1chr139900083-0.0227983240.9772017
ENST00000371102ENST00000317713GNASchr2057486244+MACF1chr139900083-0.0227983240.9772017
ENST00000371102ENST00000564288GNASchr2057486244+MACF1chr139900083-0.0251595340.9748405
ENST00000371102ENST00000289893GNASchr2057486244+MACF1chr139900083-0.0221118980.9778881
ENST00000371095ENST00000545844GNASchr2057486244+MACF1chr139900083-0.0093661890.99063385
ENST00000371095ENST00000567887GNASchr2057486244+MACF1chr139900083-0.009887750.99011225
ENST00000371095ENST00000372915GNASchr2057486244+MACF1chr139900083-0.010136290.9898637
ENST00000371095ENST00000361689GNASchr2057486244+MACF1chr139900083-0.0100084520.98999155
ENST00000371095ENST00000539005GNASchr2057486244+MACF1chr139900083-0.009493740.99050623
ENST00000371095ENST00000317713GNASchr2057486244+MACF1chr139900083-0.009493740.99050623
ENST00000371095ENST00000564288GNASchr2057486244+MACF1chr139900083-0.009887750.99011225
ENST00000371095ENST00000289893GNASchr2057486244+MACF1chr139900083-0.0092831920.99071676
ENST00000371085ENST00000545844GNASchr2057486244+MACF1chr139900083-0.009968190.99003184
ENST00000371085ENST00000567887GNASchr2057486244+MACF1chr139900083-0.0105394190.9894605
ENST00000371085ENST00000372915GNASchr2057486244+MACF1chr139900083-0.0107765330.9892235
ENST00000371085ENST00000361689GNASchr2057486244+MACF1chr139900083-0.0106410690.9893589
ENST00000371085ENST00000539005GNASchr2057486244+MACF1chr139900083-0.010093140.98990685
ENST00000371085ENST00000317713GNASchr2057486244+MACF1chr139900083-0.010093140.98990685
ENST00000371085ENST00000564288GNASchr2057486244+MACF1chr139900083-0.0105394190.9894605
ENST00000371085ENST00000289893GNASchr2057486244+MACF1chr139900083-0.0098732770.9901267
ENST00000354359ENST00000545844GNASchr2057486244+MACF1chr139900083-0.0101072470.9898928
ENST00000354359ENST00000567887GNASchr2057486244+MACF1chr139900083-0.0106868750.9893132
ENST00000354359ENST00000372915GNASchr2057486244+MACF1chr139900083-0.0109290010.989071
ENST00000354359ENST00000361689GNASchr2057486244+MACF1chr139900083-0.0107915790.9892084
ENST00000354359ENST00000539005GNASchr2057486244+MACF1chr139900083-0.0102338780.98976606
ENST00000354359ENST00000317713GNASchr2057486244+MACF1chr139900083-0.0102338780.98976606
ENST00000354359ENST00000564288GNASchr2057486244+MACF1chr139900083-0.0106868750.9893132
ENST00000354359ENST00000289893GNASchr2057486244+MACF1chr139900083-0.0100123520.9899877
ENST00000265620ENST00000545844GNASchr2057486244+MACF1chr139900083-0.0087573090.99124265
ENST00000265620ENST00000567887GNASchr2057486244+MACF1chr139900083-0.0091667730.9908332
ENST00000265620ENST00000372915GNASchr2057486244+MACF1chr139900083-0.009472050.990528
ENST00000265620ENST00000361689GNASchr2057486244+MACF1chr139900083-0.0093435710.99065644
ENST00000265620ENST00000539005GNASchr2057486244+MACF1chr139900083-0.0088651270.9911349
ENST00000265620ENST00000317713GNASchr2057486244+MACF1chr139900083-0.0088651270.9911349
ENST00000265620ENST00000564288GNASchr2057486244+MACF1chr139900083-0.0091667730.9908332
ENST00000265620ENST00000289893GNASchr2057486244+MACF1chr139900083-0.0086584020.99134165
ENST00000306090ENST00000545844GNASchr2057486244+MACF1chr139900083-0.0070284810.9929715
ENST00000306090ENST00000567887GNASchr2057486244+MACF1chr139900083-0.0070686030.99293137
ENST00000306090ENST00000372915GNASchr2057486244+MACF1chr139900083-0.0076030810.9923969
ENST00000306090ENST00000361689GNASchr2057486244+MACF1chr139900083-0.0074987540.9925013
ENST00000306090ENST00000539005GNASchr2057486244+MACF1chr139900083-0.0071127620.9928872
ENST00000306090ENST00000317713GNASchr2057486244+MACF1chr139900083-0.0071127620.9928872
ENST00000306090ENST00000564288GNASchr2057486244+MACF1chr139900083-0.0070686030.99293137
ENST00000306090ENST00000289893GNASchr2057486244+MACF1chr139900083-0.0069451210.9930549

check button Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones.

Get the fusion protein sequences from here.

Fusion protein sequence information is available in the fasta format.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

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Fusion Protein Breakpoint Sequences for GNAS-MACF1

check button +/-13 AA sequence from the breakpoints of the fusion protein sequences.
HgeneHchrHbpTgeneTchrTbpLength(fusion protein)BP in fusion proteinPeptide

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Potential FusionNeoAntigen Information of GNAS-MACF1 in HLA I

check button Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.

check button Potential FusionNeoAntigen Information
* We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5)
Fusion geneHchrHbpTgeneTchrTbpHLA IFusionNeoAntigen peptideBinding scoreImmunogenic scoreNeoantigen start (at BP 13)Neoantigen end (at BP 13)

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Potential FusionNeoAntigen Information of GNAS-MACF1 in HLA II

check button Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.

check button Potential FusionNeoAntigen Information
* We used NetMHCIIpan v4.1 (%rank<0.5).
Fusion geneHchrHbpTgeneTchrTbpHLA IIFusionNeoAntigen peptideNeoantigen start (at BP 13)Neoantigen end (at BP 13)

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Fusion breakpoint peptide structures of GNAS-MACF1

check button3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens
* The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA.

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of GNAS-MACF1

check buttonVirtual screening between 25 HLAs (from PDB) and FusionNeoAntigens
* We used Glide to predict the interaction between HLAs and neoantigens.
HLA allelePDB IDFile nameBPseqDocking scoreGlide score

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Vaccine Design for the FusionNeoAntigens of GNAS-MACF1

check button mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is.
Fusion geneHchrHbpTchrTbpStart in +/-13AAEnd in +/-13AAFusionNeoAntigen peptide sequenceFusionNeoAntigen RNA sequence

check button mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs.
Fusion geneHchrHbpTchrTbpStart in +/-13AAEnd in +/-13AAFusionNeoAntigen peptideFusionNEoAntigen RNA sequence

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Information of the samples that have these potential fusion neoantigens of GNAS-MACF1

check button These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens.
Cancer typeFusion geneHchrHbpHenstTchrTbpTenstSample

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Potential target of CAR-T therapy development for GNAS-MACF1

check button Predicted 3D structure. We used RoseTTAFold.
203_GNAS-MACF1_t000_.e2e.pdb


check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features


* Minus value of BPloci means that the break point is located before the CDS.
- In-frame and retained 'Transmembrane'.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

check button Subcellular localization prediction of the transmembrane domain retained fusion proteins
* We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image.
HgeneHchrHbpHenstTgeneTchrTbpTenstDeepLoc result
GNASchr2057486244ENST00000265620MACF1chr139900083ENST00000289893
GNASchr2057486244ENST00000265620MACF1chr139900083ENST00000317713
GNASchr2057486244ENST00000265620MACF1chr139900083ENST00000361689
GNASchr2057486244ENST00000265620MACF1chr139900083ENST00000564288

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Related Drugs to GNAS-MACF1

check button Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDrugSourcePMID

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Related Diseases to GNAS-MACF1

check button Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDiseaseSourcePMID

check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource