FusionNeoAntigen Logo

Home

Download

Statistics

Examples

Help

Contact

Terms of Use
Center for Computational Systems Medicine
leaf

Fusion Gene and Fusion Protein Summary

leaf

Fusion Amino Acid Sequences (multiple BPs and multiple gene isoforms)

leaf

Fusion Protein Breakpoint Sequences - (for the Screening of the FusionNeoAntigens)

leaf

Potential FusionNeoAntigens in HLA I - (netMHCpan v4.1 + deepHLApan v1.1)

leaf

Potential FusionNeoAntigens in HLA II - (netMHCIIpan v4.1)

leaf

Fusion Breakpoint 14 AA Peptide Structure - (RoseTTAFold)

leaf

Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D - (Glide)

leaf

Vaccine Design for the FusionNeoAntigens (RNA/protein sequences)

leaf

Potential target of CAR-T therapy development

leaf

Information on the samples that have these potential fusion neoantigens

leaf

Fusion Protein Targeting Drugs - (Manual Curation)

leaf

Fusion Protein Related diseases - (Manual Curation)

Fusion Protein:HIP1-STYXL1

Fusion Gene and Fusion Protein Summary

check button Fusion gene summary
Fusion partner gene informationFusion gene name: HIP1-STYXL1
FusionPDB ID: 36393
FusionGDB2.0 ID: 36393
HgeneTgene
Gene symbol

HIP1

STYXL1

Gene ID

3092

51657

Gene namehuntingtin interacting protein 1serine/threonine/tyrosine interacting like 1
SynonymsHIP-I|ILWEQ|SHON|SHONbeta|SHONgammaDUSP24|MK-STYX|MKSTYX
Cytomap

7q11.23

7q11.23

Type of geneprotein-codingprotein-coding
Descriptionhuntingtin-interacting protein 1huntingtin-interacting protein Iserine/threonine/tyrosine-interacting-like protein 1dual specificity phosphatase 24 (putative)dual specificity phosphatase inhibitor MK-STYXdual specificity protein phosphatase 24inactive dual specificity protein phosphatase MK-STYXmap kinase phospha
Modification date2020031320200313
UniProtAcc

O75146

Main function of 5'-partner protein: FUNCTION: Component of clathrin-coated pits and vesicles, that may link the endocytic machinery to the actin cytoskeleton. Binds 3-phosphoinositides (via ENTH domain). May act through the ENTH domain to promote cell survival by stabilizing receptor tyrosine kinases following ligand-induced endocytosis. {ECO:0000269|PubMed:11889126, ECO:0000269|PubMed:14732715}.		.
Ensembl transtripts involved in fusion geneENST idsENST00000336926, ENST00000434438, 
ENST00000479835, 		ENST00000460184, 
ENST00000248600, ENST00000340062, 
ENST00000359697, ENST00000360591, 
ENST00000431581, ENST00000451157, 
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)* DoF score6 X 9 X 5=2708 X 7 X 6=336
# samples 79
** MAII scorelog2(7/270*10)=-1.94753258010586
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(9/336*10)=-1.90046432644909
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Fusion gene context

PubMed: HIP1 [Title/Abstract] AND STYXL1 [Title/Abstract] AND fusion [Title/Abstract]

Fusion neoantigen context

PubMed: HIP1 [Title/Abstract] AND STYXL1 [Title/Abstract] AND neoantigen [Title/Abstract]

Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)HIP1(75368119)-STYXL1(75634722), # samples:2
Anticipated loss of major functional domain due to fusion event.HIP1-STYXL1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
HIP1-STYXL1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
HIP1-STYXL1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
HIP1-STYXL1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgeneHIP1

GO:0006915

apoptotic process

11788820

HgeneHIP1

GO:0006919

activation of cysteine-type endopeptidase activity involved in apoptotic process

11788820

HgeneHIP1

GO:0042981

regulation of apoptotic process

11788820

HgeneHIP1

GO:0048268

clathrin coat assembly

11577110|11889126

HgeneHIP1

GO:0050821

protein stabilization

14732715

HgeneHIP1

GO:0097190

apoptotic signaling pathway

11007801

TgeneSTYXL1

GO:0032515

negative regulation of phosphoprotein phosphatase activity

24709986



check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr7:75368119/chr7:75634722)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonRetention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here.

check buttonFusion gene breakpoints across HIP1 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check buttonFusion gene breakpoints across STYXL1 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure


Top

Fusion Amino Acid Sequences


check buttonFusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000336926HIP1chr775368119-ENST00000248600STYXL1chr775634722-76614727635202
ENST00000336926HIP1chr775368119-ENST00000359697STYXL1chr775634722-76214727635202
ENST00000336926HIP1chr775368119-ENST00000340062STYXL1chr775634722-76214727635202
ENST00000336926HIP1chr775368119-ENST00000360591STYXL1chr775634722-76114727635202
ENST00000336926HIP1chr775368119-ENST00000431581STYXL1chr775634722-69514727635202
ENST00000336926HIP1chr775368119-ENST00000451157STYXL1chr775634722-109414727542171
ENST00000434438HIP1chr775368119-ENST00000248600STYXL1chr775634722-76014121629202
ENST00000434438HIP1chr775368119-ENST00000359697STYXL1chr775634722-75614121629202
ENST00000434438HIP1chr775368119-ENST00000340062STYXL1chr775634722-75614121629202
ENST00000434438HIP1chr775368119-ENST00000360591STYXL1chr775634722-75514121629202
ENST00000434438HIP1chr775368119-ENST00000431581STYXL1chr775634722-68914121629202
ENST00000434438HIP1chr775368119-ENST00000451157STYXL1chr775634722-108814121536171
ENST00000336926HIP1chr775368118-ENST00000248600STYXL1chr775634722-76614727635202
ENST00000336926HIP1chr775368118-ENST00000359697STYXL1chr775634722-76214727635202
ENST00000336926HIP1chr775368118-ENST00000340062STYXL1chr775634722-76214727635202
ENST00000336926HIP1chr775368118-ENST00000360591STYXL1chr775634722-76114727635202
ENST00000336926HIP1chr775368118-ENST00000431581STYXL1chr775634722-69514727635202
ENST00000336926HIP1chr775368118-ENST00000451157STYXL1chr775634722-109414727542171
ENST00000434438HIP1chr775368118-ENST00000248600STYXL1chr775634722-76014121629202
ENST00000434438HIP1chr775368118-ENST00000359697STYXL1chr775634722-75614121629202
ENST00000434438HIP1chr775368118-ENST00000340062STYXL1chr775634722-75614121629202
ENST00000434438HIP1chr775368118-ENST00000360591STYXL1chr775634722-75514121629202
ENST00000434438HIP1chr775368118-ENST00000431581STYXL1chr775634722-68914121629202
ENST00000434438HIP1chr775368118-ENST00000451157STYXL1chr775634722-108814121536171

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000336926ENST00000248600HIP1chr775368119-STYXL1chr775634722-0.003129640.99687034
ENST00000336926ENST00000359697HIP1chr775368119-STYXL1chr775634722-0.0032788690.9967211
ENST00000336926ENST00000340062HIP1chr775368119-STYXL1chr775634722-0.0032788690.9967211
ENST00000336926ENST00000360591HIP1chr775368119-STYXL1chr775634722-0.0033325490.99666744
ENST00000336926ENST00000431581HIP1chr775368119-STYXL1chr775634722-0.0022062290.9977938
ENST00000336926ENST00000451157HIP1chr775368119-STYXL1chr775634722-0.0016675920.99833244
ENST00000434438ENST00000248600HIP1chr775368119-STYXL1chr775634722-0.0028124570.99718755
ENST00000434438ENST00000359697HIP1chr775368119-STYXL1chr775634722-0.0029628330.9970372
ENST00000434438ENST00000340062HIP1chr775368119-STYXL1chr775634722-0.0029628330.9970372
ENST00000434438ENST00000360591HIP1chr775368119-STYXL1chr775634722-0.0030112980.9969887
ENST00000434438ENST00000431581HIP1chr775368119-STYXL1chr775634722-0.0020683750.9979316
ENST00000434438ENST00000451157HIP1chr775368119-STYXL1chr775634722-0.0012793150.9987207
ENST00000336926ENST00000248600HIP1chr775368118-STYXL1chr775634722-0.003129640.99687034
ENST00000336926ENST00000359697HIP1chr775368118-STYXL1chr775634722-0.0032788690.9967211
ENST00000336926ENST00000340062HIP1chr775368118-STYXL1chr775634722-0.0032788690.9967211
ENST00000336926ENST00000360591HIP1chr775368118-STYXL1chr775634722-0.0033325490.99666744
ENST00000336926ENST00000431581HIP1chr775368118-STYXL1chr775634722-0.0022062290.9977938
ENST00000336926ENST00000451157HIP1chr775368118-STYXL1chr775634722-0.0016675920.99833244
ENST00000434438ENST00000248600HIP1chr775368118-STYXL1chr775634722-0.0028124570.99718755
ENST00000434438ENST00000359697HIP1chr775368118-STYXL1chr775634722-0.0029628330.9970372
ENST00000434438ENST00000340062HIP1chr775368118-STYXL1chr775634722-0.0029628330.9970372
ENST00000434438ENST00000360591HIP1chr775368118-STYXL1chr775634722-0.0030112980.9969887
ENST00000434438ENST00000431581HIP1chr775368118-STYXL1chr775634722-0.0020683750.9979316
ENST00000434438ENST00000451157HIP1chr775368118-STYXL1chr775634722-0.0012793150.9987207

check button Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones.

Get the fusion protein sequences from here.

Fusion protein sequence information is available in the fasta format.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

Top

Fusion Protein Breakpoint Sequences for HIP1-STYXL1

check button +/-13 AA sequence from the breakpoints of the fusion protein sequences.
HgeneHchrHbpTgeneTchrTbpLength(fusion protein)BP in fusion proteinPeptide
HIP1chr775368118STYXL1chr77563472214139GLEAAERESFERTQELDAFQPYPIEI
HIP1chr775368118STYXL1chr77563472214739GLEAAERESFERTQELDAFQPYPIEI
HIP1chr775368119STYXL1chr77563472214139GLEAAERESFERTQELDAFQPYPIEI
HIP1chr775368119STYXL1chr77563472214739GLEAAERESFERTQELDAFQPYPIEI

Top

Potential FusionNeoAntigen Information of HIP1-STYXL1 in HLA I

check button Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.
HIP1-STYXL1_75368118_75634722.msa

check button Potential FusionNeoAntigen Information
* We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5)
Fusion geneHchrHbpTgeneTchrTbpHLA IFusionNeoAntigen peptideBinding scoreImmunogenic scoreNeoantigen start (at BP 13)Neoantigen end (at BP 13)
HIP1-STYXL1chr775368118chr775634722147HLA-B14:02ESFERTQEL0.99620.6091716
HIP1-STYXL1chr775368118chr775634722147HLA-B14:01ESFERTQEL0.99620.6091716
HIP1-STYXL1chr775368118chr775634722147HLA-B52:01ESFERTQEL0.98850.733716
HIP1-STYXL1chr775368118chr775634722147HLA-B35:03ESFERTQEL0.9520.7477716
HIP1-STYXL1chr775368118chr775634722147HLA-B35:04ESFERTQEL0.9110.8148716
HIP1-STYXL1chr775368118chr775634722147HLA-B35:02ESFERTQEL0.9110.8148716
HIP1-STYXL1chr775368118chr775634722147HLA-B38:02ERTQELDAF0.83970.84421019
HIP1-STYXL1chr775368118chr775634722147HLA-B38:01ERTQELDAF0.83890.82531019
HIP1-STYXL1chr775368118chr775634722147HLA-B50:01FERTQELDA0.31840.7233918
HIP1-STYXL1chr775368118chr775634722147HLA-B15:01TQELDAFQPY0.99570.9131222
HIP1-STYXL1chr775368118chr775634722147HLA-B13:01RESFERTQEL0.99370.797616
HIP1-STYXL1chr775368118chr775634722147HLA-B15:25TQELDAFQPY0.96050.94141222
HIP1-STYXL1chr775368118chr775634722147HLA-B44:03RESFERTQEL0.9540.9615616
HIP1-STYXL1chr775368118chr775634722147HLA-B39:13RESFERTQEL0.9490.923616
HIP1-STYXL1chr775368118chr775634722147HLA-B15:02TQELDAFQPY0.88390.95221222
HIP1-STYXL1chr775368118chr775634722147HLA-B41:01RESFERTQEL0.85320.6576616
HIP1-STYXL1chr775368118chr775634722147HLA-B50:01RESFERTQEL0.84630.6956616
HIP1-STYXL1chr775368118chr775634722147HLA-B44:03TQELDAFQPY0.71110.94811222
HIP1-STYXL1chr775368118chr775634722147HLA-B15:03TQELDAFQPY0.63330.86911222
HIP1-STYXL1chr775368118chr775634722147HLA-B18:01TQELDAFQPY0.61170.83521222
HIP1-STYXL1chr775368118chr775634722147HLA-B14:02ERESFERTQEL0.99890.6005516
HIP1-STYXL1chr775368118chr775634722147HLA-B14:01ERESFERTQEL0.99890.6005516
HIP1-STYXL1chr775368118chr775634722147HLA-B15:17RTQELDAFQPY0.99610.9561122
HIP1-STYXL1chr775368118chr775634722147HLA-B44:03RTQELDAFQPY0.50660.83511122
HIP1-STYXL1chr775368118chr775634722147HLA-C04:07SFERTQEL0.99980.763816
HIP1-STYXL1chr775368118chr775634722147HLA-C04:10SFERTQEL0.99980.741816
HIP1-STYXL1chr775368118chr775634722147HLA-C04:14SFERTQEL0.98320.8217816
HIP1-STYXL1chr775368118chr775634722147HLA-C03:08ESFERTQEL0.99960.8402716
HIP1-STYXL1chr775368118chr775634722147HLA-C03:07ESFERTQEL0.99960.9142716
HIP1-STYXL1chr775368118chr775634722147HLA-C03:19ESFERTQEL0.99960.9709716
HIP1-STYXL1chr775368118chr775634722147HLA-C15:06ESFERTQEL0.99940.8218716
HIP1-STYXL1chr775368118chr775634722147HLA-C15:04ESFERTQEL0.99920.7938716
HIP1-STYXL1chr775368118chr775634722147HLA-C08:13ESFERTQEL0.99630.8179716
HIP1-STYXL1chr775368118chr775634722147HLA-C08:04ESFERTQEL0.99630.8179716
HIP1-STYXL1chr775368118chr775634722147HLA-C04:06ESFERTQEL0.99630.7679716
HIP1-STYXL1chr775368118chr775634722147HLA-C12:12ESFERTQEL0.99580.8446716
HIP1-STYXL1chr775368118chr775634722147HLA-C01:17ESFERTQEL0.99410.8699716
HIP1-STYXL1chr775368118chr775634722147HLA-C06:03ESFERTQEL0.9920.9788716
HIP1-STYXL1chr775368118chr775634722147HLA-B40:06FERTQELDA0.99060.5355918
HIP1-STYXL1chr775368118chr775634722147HLA-C12:04ESFERTQEL0.99010.9755716
HIP1-STYXL1chr775368118chr775634722147HLA-C08:03ESFERTQEL0.97380.8951716
HIP1-STYXL1chr775368118chr775634722147HLA-C12:16ESFERTQEL0.96590.907716
HIP1-STYXL1chr775368118chr775634722147HLA-B51:07ESFERTQEL0.96040.6343716
HIP1-STYXL1chr775368118chr775634722147HLA-C02:06ESFERTQEL0.95630.8825716
HIP1-STYXL1chr775368118chr775634722147HLA-C03:14ESFERTQEL0.95060.9446716
HIP1-STYXL1chr775368118chr775634722147HLA-C07:05ESFERTQEL0.94010.9324716
HIP1-STYXL1chr775368118chr775634722147HLA-B39:09ESFERTQEL0.93260.551716
HIP1-STYXL1chr775368118chr775634722147HLA-C01:30ESFERTQEL0.92010.8994716
HIP1-STYXL1chr775368118chr775634722147HLA-B35:12ESFERTQEL0.9110.8148716
HIP1-STYXL1chr775368118chr775634722147HLA-C07:29ESFERTQEL0.90640.8688716
HIP1-STYXL1chr775368118chr775634722147HLA-C07:13ESFERTQEL0.89930.8058716
HIP1-STYXL1chr775368118chr775634722147HLA-B39:12ERTQELDAF0.89910.72021019
HIP1-STYXL1chr775368118chr775634722147HLA-B39:10ESFERTQEL0.89510.809716
HIP1-STYXL1chr775368118chr775634722147HLA-C07:27ESFERTQEL0.89460.8893716
HIP1-STYXL1chr775368118chr775634722147HLA-C07:19ESFERTQEL0.89020.6014716
HIP1-STYXL1chr775368118chr775634722147HLA-C07:95ESFERTQEL0.86420.5562716
HIP1-STYXL1chr775368118chr775634722147HLA-B14:03ESFERTQEL0.84880.7012716
HIP1-STYXL1chr775368118chr775634722147HLA-B39:12ESFERTQEL0.84780.8295716
HIP1-STYXL1chr775368118chr775634722147HLA-B40:06RESFERTQEL0.99930.6191616
HIP1-STYXL1chr775368118chr775634722147HLA-C15:06RESFERTQEL0.99790.886616
HIP1-STYXL1chr775368118chr775634722147HLA-B39:08RESFERTQEL0.98380.6077616
HIP1-STYXL1chr775368118chr775634722147HLA-B15:07TQELDAFQPY0.95040.7391222
HIP1-STYXL1chr775368118chr775634722147HLA-B15:05TQELDAFQPY0.94150.91931222
HIP1-STYXL1chr775368118chr775634722147HLA-B15:31TQELDAFQPY0.89170.92751222
HIP1-STYXL1chr775368118chr775634722147HLA-B44:08TQELDAFQPY0.7930.59611222
HIP1-STYXL1chr775368118chr775634722147HLA-B44:09TQELDAFQPY0.77020.67331222
HIP1-STYXL1chr775368118chr775634722147HLA-B39:12ERESFERTQEL0.99920.7838516
HIP1-STYXL1chr775368118chr775634722147HLA-B14:03ERESFERTQEL0.99680.6789516
HIP1-STYXL1chr775368118chr775634722147HLA-B44:09RTQELDAFQPY0.73190.68791122
HIP1-STYXL1chr775368118chr775634722147HLA-C04:01SFERTQEL0.99980.763816
HIP1-STYXL1chr775368118chr775634722147HLA-C18:01SFERTQEL0.99960.7475816
HIP1-STYXL1chr775368118chr775634722147HLA-C04:04SFERTQEL0.99740.8585816
HIP1-STYXL1chr775368118chr775634722147HLA-C06:06SFERTQEL0.99670.9673816
HIP1-STYXL1chr775368118chr775634722147HLA-C14:03SFERTQEL0.9860.9103816
HIP1-STYXL1chr775368118chr775634722147HLA-C14:02SFERTQEL0.9860.9103816
HIP1-STYXL1chr775368118chr775634722147HLA-C03:03ESFERTQEL0.99970.968716
HIP1-STYXL1chr775368118chr775634722147HLA-C03:04ESFERTQEL0.99970.968716
HIP1-STYXL1chr775368118chr775634722147HLA-C03:05ESFERTQEL0.99960.8633716
HIP1-STYXL1chr775368118chr775634722147HLA-C03:17ESFERTQEL0.99950.9144716
HIP1-STYXL1chr775368118chr775634722147HLA-C03:67ESFERTQEL0.99940.9544716
HIP1-STYXL1chr775368118chr775634722147HLA-C15:09ESFERTQEL0.99920.7938716
HIP1-STYXL1chr775368118chr775634722147HLA-C15:05ESFERTQEL0.99920.8205716
HIP1-STYXL1chr775368118chr775634722147HLA-C15:02ESFERTQEL0.9990.763716
HIP1-STYXL1chr775368118chr775634722147HLA-A25:01ESFERTQEL0.99860.6349716
HIP1-STYXL1chr775368118chr775634722147HLA-C01:03ESFERTQEL0.99780.8309716
HIP1-STYXL1chr775368118chr775634722147HLA-C01:02ESFERTQEL0.99720.8687716
HIP1-STYXL1chr775368118chr775634722147HLA-C03:02ESFERTQEL0.99690.9202716
HIP1-STYXL1chr775368118chr775634722147HLA-C16:04ESFERTQEL0.99560.9466716
HIP1-STYXL1chr775368118chr775634722147HLA-C12:03ESFERTQEL0.99530.9427716
HIP1-STYXL1chr775368118chr775634722147HLA-C04:04ESFERTQEL0.99370.7304716
HIP1-STYXL1chr775368118chr775634722147HLA-C06:06ESFERTQEL0.99030.9571716
HIP1-STYXL1chr775368118chr775634722147HLA-C07:04ESFERTQEL0.98960.8316716
HIP1-STYXL1chr775368118chr775634722147HLA-C06:02ESFERTQEL0.98540.9747716
HIP1-STYXL1chr775368118chr775634722147HLA-C06:17ESFERTQEL0.98540.9747716
HIP1-STYXL1chr775368118chr775634722147HLA-C08:01ESFERTQEL0.97380.8951716
HIP1-STYXL1chr775368118chr775634722147HLA-C12:02ESFERTQEL0.96780.9159716
HIP1-STYXL1chr775368118chr775634722147HLA-C07:22ESFERTQEL0.96410.5844716
HIP1-STYXL1chr775368118chr775634722147HLA-C03:06ESFERTQEL0.96170.9672716
HIP1-STYXL1chr775368118chr775634722147HLA-B35:13ESFERTQEL0.95660.7597716
HIP1-STYXL1chr775368118chr775634722147HLA-C02:10ESFERTQEL0.95170.9074716
HIP1-STYXL1chr775368118chr775634722147HLA-C02:02ESFERTQEL0.95170.9074716
HIP1-STYXL1chr775368118chr775634722147HLA-C06:08ESFERTQEL0.94910.9423716
HIP1-STYXL1chr775368118chr775634722147HLA-C16:01ESFERTQEL0.93090.9426716
HIP1-STYXL1chr775368118chr775634722147HLA-C16:02ESFERTQEL0.92260.9803716
HIP1-STYXL1chr775368118chr775634722147HLA-B39:31ERTQELDAF0.91330.71171019
HIP1-STYXL1chr775368118chr775634722147HLA-B35:09ESFERTQEL0.9110.8148716
HIP1-STYXL1chr775368118chr775634722147HLA-C07:01ESFERTQEL0.8650.5708716
HIP1-STYXL1chr775368118chr775634722147HLA-B38:05ERTQELDAF0.83890.82531019
HIP1-STYXL1chr775368118chr775634722147HLA-B07:13ESFERTQEL0.80110.6824716
HIP1-STYXL1chr775368118chr775634722147HLA-C17:01ESFERTQEL0.73590.62716
HIP1-STYXL1chr775368118chr775634722147HLA-B15:08ESFERTQEL0.69130.7109716
HIP1-STYXL1chr775368118chr775634722147HLA-B35:43ESFERTQEL0.68870.7102716
HIP1-STYXL1chr775368118chr775634722147HLA-B50:04FERTQELDA0.31840.7233918
HIP1-STYXL1chr775368118chr775634722147HLA-B50:05FERTQELDA0.31840.7233918
HIP1-STYXL1chr775368118chr775634722147HLA-B40:04RESFERTQEL0.99890.6264616
HIP1-STYXL1chr775368118chr775634722147HLA-C15:05RESFERTQEL0.99810.8841616
HIP1-STYXL1chr775368118chr775634722147HLA-C15:02RESFERTQEL0.99760.8219616
HIP1-STYXL1chr775368118chr775634722147HLA-B15:125TQELDAFQPY0.99570.9131222
HIP1-STYXL1chr775368118chr775634722147HLA-B15:33TQELDAFQPY0.99570.9131222
HIP1-STYXL1chr775368118chr775634722147HLA-B15:34TQELDAFQPY0.99570.9131222
HIP1-STYXL1chr775368118chr775634722147HLA-B15:27TQELDAFQPY0.99550.9191222
HIP1-STYXL1chr775368118chr775634722147HLA-B15:135TQELDAFQPY0.99370.92181222
HIP1-STYXL1chr775368118chr775634722147HLA-B15:50TQELDAFQPY0.99140.88191222
HIP1-STYXL1chr775368118chr775634722147HLA-B15:12TQELDAFQPY0.98660.85641222
HIP1-STYXL1chr775368118chr775634722147HLA-B15:53FERTQELDAF0.98520.7453919
HIP1-STYXL1chr775368118chr775634722147HLA-B15:53RESFERTQEL0.97450.8748616
HIP1-STYXL1chr775368118chr775634722147HLA-B39:02RESFERTQEL0.96840.9224616
HIP1-STYXL1chr775368118chr775634722147HLA-B18:11FERTQELDAF0.96590.6264919
HIP1-STYXL1chr775368118chr775634722147HLA-B15:53TQELDAFQPY0.96550.89371222
HIP1-STYXL1chr775368118chr775634722147HLA-B15:39TQELDAFQPY0.9640.90241222
HIP1-STYXL1chr775368118chr775634722147HLA-B44:26RESFERTQEL0.9540.9615616
HIP1-STYXL1chr775368118chr775634722147HLA-B44:13RESFERTQEL0.9540.9615616
HIP1-STYXL1chr775368118chr775634722147HLA-B44:07RESFERTQEL0.9540.9615616
HIP1-STYXL1chr775368118chr775634722147HLA-B15:35TQELDAFQPY0.94270.89951222
HIP1-STYXL1chr775368118chr775634722147HLA-B15:20TQELDAFQPY0.94140.9441222
HIP1-STYXL1chr775368118chr775634722147HLA-B35:28TQELDAFQPY0.92750.95141222
HIP1-STYXL1chr775368118chr775634722147HLA-B48:02RESFERTQEL0.91970.9141616
HIP1-STYXL1chr775368118chr775634722147HLA-B35:20TQELDAFQPY0.87510.95741222
HIP1-STYXL1chr775368118chr775634722147HLA-B15:54TQELDAFQPY0.86880.88291222
HIP1-STYXL1chr775368118chr775634722147HLA-B50:05RESFERTQEL0.84630.6956616
HIP1-STYXL1chr775368118chr775634722147HLA-B50:04RESFERTQEL0.84630.6956616
HIP1-STYXL1chr775368118chr775634722147HLA-B18:11TQELDAFQPY0.78490.8531222
HIP1-STYXL1chr775368118chr775634722147HLA-B18:04TQELDAFQPY0.74370.84781222
HIP1-STYXL1chr775368118chr775634722147HLA-B48:02TQELDAFQPY0.73680.93931222
HIP1-STYXL1chr775368118chr775634722147HLA-B44:26TQELDAFQPY0.71110.94811222
HIP1-STYXL1chr775368118chr775634722147HLA-B44:13TQELDAFQPY0.71110.94811222
HIP1-STYXL1chr775368118chr775634722147HLA-B44:07TQELDAFQPY0.71110.94811222
HIP1-STYXL1chr775368118chr775634722147HLA-B18:08TQELDAFQPY0.62030.85861222
HIP1-STYXL1chr775368118chr775634722147HLA-B18:05TQELDAFQPY0.61170.83521222
HIP1-STYXL1chr775368118chr775634722147HLA-B18:06TQELDAFQPY0.52960.85111222
HIP1-STYXL1chr775368118chr775634722147HLA-B18:03TQELDAFQPY0.49250.82431222
HIP1-STYXL1chr775368118chr775634722147HLA-B39:31ERESFERTQEL0.99950.776516
HIP1-STYXL1chr775368118chr775634722147HLA-B48:02RTQELDAFQPY0.66810.90151122
HIP1-STYXL1chr775368118chr775634722147HLA-B44:07RTQELDAFQPY0.50660.83511122
HIP1-STYXL1chr775368118chr775634722147HLA-B44:13RTQELDAFQPY0.50660.83511122
HIP1-STYXL1chr775368118chr775634722147HLA-B44:26RTQELDAFQPY0.50660.83511122

Top

Potential FusionNeoAntigen Information of HIP1-STYXL1 in HLA II

check button Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.
HIP1-STYXL1_75368118_75634722.msa

check button Potential FusionNeoAntigen Information
* We used NetMHCIIpan v4.1 (%rank<0.5).
Fusion geneHchrHbpTgeneTchrTbpHLA IIFusionNeoAntigen peptideNeoantigen start (at BP 13)Neoantigen end (at BP 13)
HIP1-STYXL1chr775368118chr775634722147DRB1-1512RTQELDAFQPYPIEI1126

Top

Fusion breakpoint peptide structures of HIP1-STYXL1

check button3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens
* The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA.
File nameBPseqHgeneTgeneHchrHbpTchrTbpAAlen
7806RESFERTQELDAFQHIP1STYXL1chr775368118chr775634722147

Top

Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of HIP1-STYXL1

check buttonVirtual screening between 25 HLAs (from PDB) and FusionNeoAntigens
* We used Glide to predict the interaction between HLAs and neoantigens.
HLA allelePDB IDFile nameBPseqDocking scoreGlide score
HLA-B14:023BVN7806RESFERTQELDAFQ-6.01316-6.12656
HLA-B14:023BVN7806RESFERTQELDAFQ-3.92858-4.96388
HLA-B52:013W397806RESFERTQELDAFQ-5.92102-6.95632
HLA-B52:013W397806RESFERTQELDAFQ-4.84472-4.95812
HLA-A24:025HGA7806RESFERTQELDAFQ-8.26357-9.29887
HLA-A24:025HGA7806RESFERTQELDAFQ-7.03366-7.14706
HLA-B44:053DX87806RESFERTQELDAFQ-6.13971-6.25311
HLA-B44:053DX87806RESFERTQELDAFQ-5.20728-6.24258
HLA-A02:016TDR7806RESFERTQELDAFQ-5.28864-5.40204

Top

Vaccine Design for the FusionNeoAntigens of HIP1-STYXL1

check button mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is.
Fusion geneHchrHbpTchrTbpStart in +/-13AAEnd in +/-13AAFusionNeoAntigen peptide sequenceFusionNeoAntigen RNA sequence
HIP1-STYXL1chr775368118chr7756347221019ERTQELDAFCGGACTCAGGAACTGGATGCATTTCAG
HIP1-STYXL1chr775368118chr7756347221122RTQELDAFQPYACTCAGGAACTGGATGCATTTCAGCCATACCCC
HIP1-STYXL1chr775368118chr7756347221222TQELDAFQPYCAGGAACTGGATGCATTTCAGCCATACCCC
HIP1-STYXL1chr775368118chr775634722516ERESFERTQELCGCGAGAGCTTCGAGCGGACTCAGGAACTGGAT
HIP1-STYXL1chr775368118chr775634722616RESFERTQELGAGAGCTTCGAGCGGACTCAGGAACTGGAT
HIP1-STYXL1chr775368118chr775634722716ESFERTQELAGCTTCGAGCGGACTCAGGAACTGGAT
HIP1-STYXL1chr775368118chr775634722816SFERTQELTTCGAGCGGACTCAGGAACTGGAT
HIP1-STYXL1chr775368118chr775634722918FERTQELDAGAGCGGACTCAGGAACTGGATGCATTT
HIP1-STYXL1chr775368118chr775634722919FERTQELDAFGAGCGGACTCAGGAACTGGATGCATTTCAG

check button mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs.
Fusion geneHchrHbpTchrTbpStart in +/-13AAEnd in +/-13AAFusionNeoAntigen peptideFusionNEoAntigen RNA sequence
HIP1-STYXL1chr775368118chr7756347221126RTQELDAFQPYPIEIACTCAGGAACTGGATGCATTTCAGCCATACCCCATTGAAATCGTG

Top

Information of the samples that have these potential fusion neoantigens of HIP1-STYXL1

check button These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens.
Cancer typeFusion geneHchrHbpHenstTchrTbpTenstSample
OVHIP1-STYXL1chr775368118ENST00000336926chr775634722ENST00000248600TCGA-24-1558

Top

Potential target of CAR-T therapy development for HIP1-STYXL1

check button Predicted 3D structure. We used RoseTTAFold.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features


* Minus value of BPloci means that the break point is located before the CDS.
- In-frame and retained 'Transmembrane'.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

check button Subcellular localization prediction of the transmembrane domain retained fusion proteins
* We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image.
HgeneHchrHbpHenstTgeneTchrTbpTenstDeepLoc result

Top

Related Drugs to HIP1-STYXL1

check button Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDrugSourcePMID

Top

Related Diseases to HIP1-STYXL1

check button Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDiseaseSourcePMID

check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource
HgeneHIP1C0023893Liver Cirrhosis, Experimental1CTD_human