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Center for Computational Systems Medicine
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Fusion Gene and Fusion Protein Summary

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Fusion Amino Acid Sequences (multiple BPs and multiple gene isoforms)

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Fusion Protein Breakpoint Sequences - (for the Screening of the FusionNeoAntigens)

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Potential FusionNeoAntigens in HLA I - (netMHCpan v4.1 + deepHLApan v1.1)

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Potential FusionNeoAntigens in HLA II - (netMHCIIpan v4.1)

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Fusion Breakpoint 14 AA Peptide Structure - (RoseTTAFold)

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D - (Glide)

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Vaccine Design for the FusionNeoAntigens (RNA/protein sequences)

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Potential target of CAR-T therapy development

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Information on the samples that have these potential fusion neoantigens

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Fusion Protein Targeting Drugs - (Manual Curation)

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Fusion Protein Related diseases - (Manual Curation)

Fusion Protein:HP-CFB

Fusion Gene and Fusion Protein Summary

check button Fusion gene summary
Fusion partner gene informationFusion gene name: HP-CFB
FusionPDB ID: 37494
FusionGDB2.0 ID: 37494
HgeneTgene
Gene symbol

HP

CFB

Gene ID

10395

629

Gene nameDLC1 Rho GTPase activating proteincomplement factor B
SynonymsARHGAP7|HP|STARD12|p122-RhoGAPAHUS4|ARMD14|BF|BFD|CFAB|CFBD|FB|FBI12|GBG|H2-Bf|PBF2
Cytomap

8p22

6p21.33

Type of geneprotein-codingprotein-coding
Descriptionrho GTPase-activating protein 7Rho-GTPase-activating protein 7START domain-containing protein 12StAR-related lipid transfer (START) domain containing 12deleted in liver cancer 1 proteindeleted in liver cancer 1 variant 2deleted in liver cancer variacomplement factor BB-factor, properdinC3 proacceleratorC3 proactivatorC3/C5 convertaseglycine-rich beta-glycoproteinproperdin factor B
Modification date2020031320200327
UniProtAcc

P02790

Main function of 5'-partner protein: FUNCTION: Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.

P00751

Main function of 5'-partner protein: FUNCTION: Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase. It has also been implicated in proliferation and differentiation of preactivated B-lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes. Ba inhibits the proliferation of preactivated B-lymphocytes.
Ensembl transtripts involved in fusion geneENST idsENST00000355906, ENST00000357763, 
ENST00000398131, ENST00000562526, 
ENST00000569639, ENST00000570083, 
ENST00000565574, 		ENST00000375455, 
ENST00000399981, ENST00000417261, 
ENST00000419411, ENST00000419920, 
ENST00000424727, ENST00000426239, 
ENST00000427888, ENST00000433503, 
ENST00000436692, ENST00000455591, 
ENST00000459734, ENST00000460958, 
ENST00000465211, ENST00000473139, 
ENST00000474621, ENST00000489605, 
ENST00000497841, ENST00000425368, 
ENST00000456570, ENST00000477310, 
ENST00000556679, 
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)* DoF score9 X 11 X 3=29717 X 11 X 9=1683
# samples 1020
** MAII scorelog2(10/297*10)=-1.57046293102604
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(20/1683*10)=-3.07296327155522
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Fusion gene context

PubMed: HP [Title/Abstract] AND CFB [Title/Abstract] AND fusion [Title/Abstract]

Fusion neoantigen context

PubMed: HP [Title/Abstract] AND CFB [Title/Abstract] AND neoantigen [Title/Abstract]

Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)HP(72093066)-CFB(31918944), # samples:1
Anticipated loss of major functional domain due to fusion event.HP-CFB seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
HP-CFB seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
HP-CFB seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
HP-CFB seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgeneHP

GO:0006915

apoptotic process

17292327

HgeneHP

GO:0006919

activation of cysteine-type endopeptidase activity involved in apoptotic process

17888903

HgeneHP

GO:0008285

negative regulation of cell proliferation

12545165|17932950

HgeneHP

GO:0030336

negative regulation of cell migration

17932950|19158340

HgeneHP

GO:0035307

positive regulation of protein dephosphorylation

17292327

HgeneHP

GO:0051497

negative regulation of stress fiber assembly

17932950

HgeneHP

GO:0051895

negative regulation of focal adhesion assembly

19158340

HgeneHP

GO:1900119

positive regulation of execution phase of apoptosis

17888903



check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr16:72093066/chr6:31918944)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonRetention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here.

check buttonFusion gene breakpoints across HP (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check buttonFusion gene breakpoints across CFB (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure


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Fusion Amino Acid Sequences


check buttonFusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000570083HPchr1672093066+ENST00000556679CFBchr631918944+70627219687222
ENST00000570083HPchr1672093066+ENST00000456570CFBchr631918944+70627219687222
ENST00000570083HPchr1672093066+ENST00000477310CFBchr631918944+68827219687223
ENST00000570083HPchr1672093066+ENST00000425368CFBchr631918944+74227219687222
ENST00000355906HPchr1672093066+ENST00000556679CFBchr631918944+88044616861281
ENST00000355906HPchr1672093066+ENST00000456570CFBchr631918944+88044616861281
ENST00000355906HPchr1672093066+ENST00000477310CFBchr631918944+86244616861282
ENST00000355906HPchr1672093066+ENST00000425368CFBchr631918944+91644616861281
ENST00000398131HPchr1672093066+ENST00000556679CFBchr631918944+6892552670222
ENST00000398131HPchr1672093066+ENST00000456570CFBchr631918944+6892552670222
ENST00000398131HPchr1672093066+ENST00000477310CFBchr631918944+6712552670223
ENST00000398131HPchr1672093066+ENST00000425368CFBchr631918944+7252552670222
ENST00000569639HPchr1672093066+ENST00000556679CFBchr631918944+948514525929134
ENST00000569639HPchr1672093066+ENST00000456570CFBchr631918944+948514525929134
ENST00000569639HPchr1672093066+ENST00000477310CFBchr631918944+930514525929134
ENST00000569639HPchr1672093066+ENST00000425368CFBchr631918944+984514525929134
ENST00000357763HPchr1672093066+ENST00000556679CFBchr631918944+95752327938303
ENST00000357763HPchr1672093066+ENST00000456570CFBchr631918944+95752327938303
ENST00000357763HPchr1672093066+ENST00000477310CFBchr631918944+93952327938303
ENST00000357763HPchr1672093066+ENST00000425368CFBchr631918944+99352327938303
ENST00000562526HPchr1672093066+ENST00000556679CFBchr631918944+67223827653208
ENST00000562526HPchr1672093066+ENST00000456570CFBchr631918944+67223827653208
ENST00000562526HPchr1672093066+ENST00000477310CFBchr631918944+65423827653208
ENST00000562526HPchr1672093066+ENST00000425368CFBchr631918944+70823827653208

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000570083ENST00000556679HPchr1672093066+CFBchr631918944+0.0059286260.9940713
ENST00000570083ENST00000456570HPchr1672093066+CFBchr631918944+0.0059286260.9940713
ENST00000570083ENST00000477310HPchr1672093066+CFBchr631918944+0.006129380.9938706
ENST00000570083ENST00000425368HPchr1672093066+CFBchr631918944+0.006043170.9939568
ENST00000355906ENST00000556679HPchr1672093066+CFBchr631918944+0.0059548680.99404514
ENST00000355906ENST00000456570HPchr1672093066+CFBchr631918944+0.0059548680.99404514
ENST00000355906ENST00000477310HPchr1672093066+CFBchr631918944+0.0063461130.9936539
ENST00000355906ENST00000425368HPchr1672093066+CFBchr631918944+0.0056165360.99438345
ENST00000398131ENST00000556679HPchr1672093066+CFBchr631918944+0.0062018330.9937982
ENST00000398131ENST00000456570HPchr1672093066+CFBchr631918944+0.0062018330.9937982
ENST00000398131ENST00000477310HPchr1672093066+CFBchr631918944+0.0065468280.99345315
ENST00000398131ENST00000425368HPchr1672093066+CFBchr631918944+0.0059500780.9940499
ENST00000569639ENST00000556679HPchr1672093066+CFBchr631918944+0.154528110.84547186
ENST00000569639ENST00000456570HPchr1672093066+CFBchr631918944+0.154528110.84547186
ENST00000569639ENST00000477310HPchr1672093066+CFBchr631918944+0.152707710.8472923
ENST00000569639ENST00000425368HPchr1672093066+CFBchr631918944+0.161904420.8380956
ENST00000357763ENST00000556679HPchr1672093066+CFBchr631918944+0.0030777540.9969222
ENST00000357763ENST00000456570HPchr1672093066+CFBchr631918944+0.0030777540.9969222
ENST00000357763ENST00000477310HPchr1672093066+CFBchr631918944+0.0031928580.9968072
ENST00000357763ENST00000425368HPchr1672093066+CFBchr631918944+0.0029204580.9970796
ENST00000562526ENST00000556679HPchr1672093066+CFBchr631918944+0.0051327960.9948672
ENST00000562526ENST00000456570HPchr1672093066+CFBchr631918944+0.0051327960.9948672
ENST00000562526ENST00000477310HPchr1672093066+CFBchr631918944+0.0052876330.99471235
ENST00000562526ENST00000425368HPchr1672093066+CFBchr631918944+0.0047203760.99527967

check button Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones.

Get the fusion protein sequences from here.

Fusion protein sequence information is available in the fasta format.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

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Fusion Protein Breakpoint Sequences for HP-CFB

check button +/-13 AA sequence from the breakpoints of the fusion protein sequences.
HgeneHchrHbpTgeneTchrTbpLength(fusion protein)BP in fusion proteinPeptide
HPchr1672093066CFBchr63191894423870LRTEGDELPECEADIKALFVSEEEKK
HPchr1672093066CFBchr63191894425584LRTEGDELPECEADIKALFVSEEEKK
HPchr1672093066CFBchr63191894427284LRTEGDELPECEADIKALFVSEEEKK
HPchr1672093066CFBchr631918944446143LRTEGDELPECEADIKALFVSEEEKK
HPchr1672093066CFBchr631918944523165MMSPSPKLPECEADIKALFVSEEEKK

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Potential FusionNeoAntigen Information of HP-CFB in HLA I

check button Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.
HP-CFB_72093066_31918944.msa

check button Potential FusionNeoAntigen Information
* We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5)
Fusion geneHchrHbpTgeneTchrTbpHLA IFusionNeoAntigen peptideBinding scoreImmunogenic scoreNeoantigen start (at BP 13)Neoantigen end (at BP 13)
HP-CFBchr1672093066chr631918944523HLA-B44:03CEADIKALF0.99920.75381019
HP-CFBchr1672093066chr631918944523HLA-B18:01CEADIKALF0.99890.8091019
HP-CFBchr1672093066chr631918944523HLA-B13:01CEADIKALF0.99550.52351019
HP-CFBchr1672093066chr631918944523HLA-B44:03ECEADIKALF0.93810.8079919
HP-CFBchr1672093066chr631918944523HLA-B18:01ECEADIKALF0.6980.7031919
HP-CFBchr1672093066chr631918944523HLA-C05:09EADIKALFV0.99990.85771120
HP-CFBchr1672093066chr631918944523HLA-C08:15EADIKALFV0.99960.93081120
HP-CFBchr1672093066chr631918944523HLA-C04:06EADIKALFV0.98580.68971120
HP-CFBchr1672093066chr631918944523HLA-C05:01EADIKALFV0.99990.85771120
HP-CFBchr1672093066chr631918944523HLA-C04:03EADIKALFV0.99990.74491120
HP-CFBchr1672093066chr631918944523HLA-C08:02EADIKALFV0.99960.93081120
HP-CFBchr1672093066chr631918944523HLA-B18:07CEADIKALF0.99930.77061019
HP-CFBchr1672093066chr631918944523HLA-B44:07CEADIKALF0.99920.75381019
HP-CFBchr1672093066chr631918944523HLA-B44:13CEADIKALF0.99920.75381019
HP-CFBchr1672093066chr631918944523HLA-B44:26CEADIKALF0.99920.75381019
HP-CFBchr1672093066chr631918944523HLA-B18:04CEADIKALF0.99920.8221019
HP-CFBchr1672093066chr631918944523HLA-B18:08CEADIKALF0.99890.58881019
HP-CFBchr1672093066chr631918944523HLA-B18:05CEADIKALF0.99890.8091019
HP-CFBchr1672093066chr631918944523HLA-B18:06CEADIKALF0.99850.81381019
HP-CFBchr1672093066chr631918944523HLA-B18:03CEADIKALF0.99820.80011019
HP-CFBchr1672093066chr631918944523HLA-B18:11CEADIKALF0.9430.71421019
HP-CFBchr1672093066chr631918944523HLA-B35:20CEADIKALF0.85990.61671019
HP-CFBchr1672093066chr631918944523HLA-B48:02CEADIKALF0.56970.58421019
HP-CFBchr1672093066chr631918944523HLA-B15:53CEADIKALF0.33960.58031019
HP-CFBchr1672093066chr631918944523HLA-B44:07ECEADIKALF0.93810.8079919
HP-CFBchr1672093066chr631918944523HLA-B44:26ECEADIKALF0.93810.8079919
HP-CFBchr1672093066chr631918944523HLA-B44:13ECEADIKALF0.93810.8079919
HP-CFBchr1672093066chr631918944523HLA-B18:11ECEADIKALF0.90510.6072919
HP-CFBchr1672093066chr631918944523HLA-B18:04ECEADIKALF0.84980.7386919
HP-CFBchr1672093066chr631918944523HLA-B18:06ECEADIKALF0.71340.7145919
HP-CFBchr1672093066chr631918944523HLA-B18:05ECEADIKALF0.6980.7031919
HP-CFBchr1672093066chr631918944523HLA-B18:08ECEADIKALF0.67920.5104919
HP-CFBchr1672093066chr631918944523HLA-B18:03ECEADIKALF0.67130.6878919

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Potential FusionNeoAntigen Information of HP-CFB in HLA II

check button Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.
HP-CFB_72093066_31918944.msa

check button Potential FusionNeoAntigen Information
* We used NetMHCIIpan v4.1 (%rank<0.5).
Fusion geneHchrHbpTgeneTchrTbpHLA IIFusionNeoAntigen peptideNeoantigen start (at BP 13)Neoantigen end (at BP 13)
HP-CFBchr1672093066chr631918944446DRB1-0403EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944523DRB1-0403EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944446DRB1-0405EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944523DRB1-0405EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944446DRB1-0411EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944446DRB1-0411CEADIKALFVSEEEK1025
HP-CFBchr1672093066chr631918944523DRB1-0411EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944523DRB1-0411CEADIKALFVSEEEK1025
HP-CFBchr1672093066chr631918944446DRB1-0417EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944523DRB1-0417EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944446DRB1-0424EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944523DRB1-0424EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944446DRB1-0427EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944523DRB1-0427EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944446DRB1-0429EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944523DRB1-0429EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944446DRB1-0430EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944523DRB1-0430EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944446DRB1-0439EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944523DRB1-0439EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944446DRB1-0441EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944523DRB1-0441EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944446DRB1-0445EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944523DRB1-0445EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944446DRB1-0446EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944523DRB1-0446EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944446DRB1-0448EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944523DRB1-0448EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944446DRB1-0449EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944523DRB1-0449EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944446DRB1-0450EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944523DRB1-0450EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944446DRB1-0451EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944523DRB1-0451EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944446DRB1-0452EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944523DRB1-0452EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944446DRB1-0457EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944523DRB1-0457EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944446DRB1-0459EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944523DRB1-0459EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944446DRB1-0460EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944523DRB1-0460EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944446DRB1-0467EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944446DRB1-0467CEADIKALFVSEEEK1025
HP-CFBchr1672093066chr631918944523DRB1-0467EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944523DRB1-0467CEADIKALFVSEEEK1025
HP-CFBchr1672093066chr631918944446DRB1-0471EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944523DRB1-0471EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944446DRB1-0477EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944523DRB1-0477EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944446DRB1-0480EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944523DRB1-0480EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944446DRB1-0483EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944523DRB1-0483EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944446DRB1-0484EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944523DRB1-0484EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944446DRB1-0485EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944523DRB1-0485EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944446DRB1-0487EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944446DRB1-0487CEADIKALFVSEEEK1025
HP-CFBchr1672093066chr631918944523DRB1-0487EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944523DRB1-0487CEADIKALFVSEEEK1025
HP-CFBchr1672093066chr631918944446DRB1-0488EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944523DRB1-0488EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944446DRB1-0489EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944523DRB1-0489EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944446DRB1-0491EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944446DRB1-0491CEADIKALFVSEEEK1025
HP-CFBchr1672093066chr631918944523DRB1-0491EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944523DRB1-0491CEADIKALFVSEEEK1025
HP-CFBchr1672093066chr631918944446DRB1-1002EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944523DRB1-1002EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944446DRB1-1410EADIKALFVSEEEKK1126
HP-CFBchr1672093066chr631918944523DRB1-1410EADIKALFVSEEEKK1126

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Fusion breakpoint peptide structures of HP-CFB

check button3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens
* The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA.
File nameBPseqHgeneTgeneHchrHbpTchrTbpAAlen
4397KLPECEADIKALFVHPCFBchr1672093066chr631918944523

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of HP-CFB

check buttonVirtual screening between 25 HLAs (from PDB) and FusionNeoAntigens
* We used Glide to predict the interaction between HLAs and neoantigens.
HLA allelePDB IDFile nameBPseqDocking scoreGlide score
HLA-B14:023BVN4397KLPECEADIKALFV-7.9962-8.1096
HLA-B14:023BVN4397KLPECEADIKALFV-5.70842-6.74372
HLA-B52:013W394397KLPECEADIKALFV-6.83737-6.95077
HLA-B52:013W394397KLPECEADIKALFV-4.4836-5.5189
HLA-A11:014UQ24397KLPECEADIKALFV-10.0067-10.1201
HLA-A11:014UQ24397KLPECEADIKALFV-9.03915-10.0745
HLA-A24:025HGA4397KLPECEADIKALFV-6.56204-6.67544
HLA-A24:025HGA4397KLPECEADIKALFV-5.42271-6.45801
HLA-B44:053DX84397KLPECEADIKALFV-7.85648-8.89178
HLA-B44:053DX84397KLPECEADIKALFV-5.3978-5.5112
HLA-A02:016TDR4397KLPECEADIKALFV-3.37154-4.40684

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Vaccine Design for the FusionNeoAntigens of HP-CFB

check button mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is.
Fusion geneHchrHbpTchrTbpStart in +/-13AAEnd in +/-13AAFusionNeoAntigen peptide sequenceFusionNeoAntigen RNA sequence
HP-CFBchr1672093066chr6319189441019CEADIKALFGTGAAGCAGATATCAAAGCTCTGTTTG
HP-CFBchr1672093066chr6319189441120EADIKALFVAAGCAGATATCAAAGCTCTGTTTGTGT
HP-CFBchr1672093066chr631918944919ECEADIKALFAATGTGAAGCAGATATCAAAGCTCTGTTTG

check button mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs.
Fusion geneHchrHbpTchrTbpStart in +/-13AAEnd in +/-13AAFusionNeoAntigen peptideFusionNEoAntigen RNA sequence
HP-CFBchr1672093066chr6319189441025CEADIKALFVSEEEKGTGAAGCAGATATCAAAGCTCTGTTTGTGTCTGAGGAGGAGAAAA
HP-CFBchr1672093066chr6319189441126EADIKALFVSEEEKKAAGCAGATATCAAAGCTCTGTTTGTGTCTGAGGAGGAGAAAAAGC

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Information of the samples that have these potential fusion neoantigens of HP-CFB

check button These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens.
Cancer typeFusion geneHchrHbpHenstTchrTbpTenstSample
N/AHP-CFBchr1672093066ENST00000357763chr631918944ENST00000425368BC007464

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Potential target of CAR-T therapy development for HP-CFB

check button Predicted 3D structure. We used RoseTTAFold.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features


* Minus value of BPloci means that the break point is located before the CDS.
- In-frame and retained 'Transmembrane'.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

check button Subcellular localization prediction of the transmembrane domain retained fusion proteins
* We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image.
HgeneHchrHbpHenstTgeneTchrTbpTenstDeepLoc result

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Related Drugs to HP-CFB

check button Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDrugSourcePMID

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Related Diseases to HP-CFB

check button Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDiseaseSourcePMID

check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource