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Fusion Protein:ALPK1-NAA15 |
Fusion Gene and Fusion Protein Summary |
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Fusion partner gene information | Fusion gene name: ALPK1-NAA15 | FusionPDB ID: 4109 | FusionGDB2.0 ID: 4109 | Hgene | Tgene | Gene symbol | ALPK1 | NAA15 | Gene ID | 80216 | 80155 |
Gene name | alpha kinase 1 | N-alpha-acetyltransferase 15, NatA auxiliary subunit | |
Synonyms | 8430410J10Rik|LAK | Ga19|MRD50|NARG1|NAT1P|NATH|TBDN|TBDN100 | |
Cytomap | 4q25 | 4q31.1 | |
Type of gene | protein-coding | protein-coding | |
Description | alpha-protein kinase 1chromosome 4 kinaselymphocyte alpha-kinaselymphocyte alpha-protein kinase | N-alpha-acetyltransferase 15, NatA auxiliary subunitN-terminal acetyltransferaseNMDA receptor regulated 1NMDA receptor-regulated protein 1gastric cancer antigen Ga19protein tubedown-1transcriptional coactivator tubedown-100tubedown-1 | |
Modification date | 20200320 | 20200313 | |
UniProtAcc | Q96QP1 Main function of 5'-partner protein: FUNCTION: Serine/threonine-protein kinase that detects bacterial pathogen-associated molecular pattern metabolites (PAMPs) and initiates an innate immune response, a critical step for pathogen elimination and engagement of adaptive immunity (PubMed:28877472, PubMed:28222186, PubMed:30111836). Specifically recognizes and binds ADP-D-glycero-beta-D-manno-heptose (ADP-Heptose), a potent PAMP present in all Gram-negative and some Gram-positive bacteria (PubMed:30111836). ADP-Heptose-binding stimulates its kinase activity to phosphorylate and activate TIFA, triggering proinflammatory NF-kappa-B signaling (PubMed:30111836). May be involved in monosodium urate monohydrate (MSU)-induced inflammation by mediating phosphorylation of unconventional myosin MYO9A (PubMed:27169898). May also play a role in apical protein transport by mediating phosphorylation of unconventional myosin MYO1A (PubMed:15883161). {ECO:0000269|PubMed:15883161, ECO:0000269|PubMed:27169898, ECO:0000269|PubMed:28222186, ECO:0000269|PubMed:28877472, ECO:0000269|PubMed:30111836}. | Q9BXJ9 Main function of 5'-partner protein: FUNCTION: Auxillary subunit of the N-terminal acetyltransferase A (NatA) complex which displays alpha (N-terminal) acetyltransferase activity. The NAT activity may be important for vascular, hematopoietic and neuronal growth and development. Required to control retinal neovascularization in adult ocular endothelial cells. In complex with XRCC6 and XRCC5 (Ku80), up-regulates transcription from the osteocalcin promoter. {ECO:0000269|PubMed:11687548, ECO:0000269|PubMed:12145306, ECO:0000269|PubMed:15496142}. | |
Ensembl transtripts involved in fusion gene | ENST ids | ENST00000177648, ENST00000458497, ENST00000504176, ENST00000505912, | ENST00000480277, ENST00000515576, ENST00000296543, ENST00000398947, |
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0) | * DoF score | 6 X 6 X 3=108 | 14 X 11 X 8=1232 |
# samples | 6 | 15 | |
** MAII score | log2(6/108*10)=-0.84799690655495 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | log2(15/1232*10)=-3.03796785019902 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | |
Fusion gene context | PubMed: ALPK1 [Title/Abstract] AND NAA15 [Title/Abstract] AND fusion [Title/Abstract] | ||
Fusion neoantigen context | PubMed: ALPK1 [Title/Abstract] AND NAA15 [Title/Abstract] AND neoantigen [Title/Abstract] | ||
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0) | ALPK1(113362261)-NAA15(140291365), # samples:1 | ||
Anticipated loss of major functional domain due to fusion event. | ALPK1-NAA15 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. ALPK1-NAA15 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. ALPK1-NAA15 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. ALPK1-NAA15 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. |
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
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Partner | Gene | GO ID | GO term | PubMed ID |
Hgene | ALPK1 | GO:0002753 | cytoplasmic pattern recognition receptor signaling pathway | 28222186|28877472|30111836 |
Hgene | ALPK1 | GO:0043123 | positive regulation of I-kappaB kinase/NF-kappaB signaling | 28222186|28877472|30111836 |
Hgene | ALPK1 | GO:0045087 | innate immune response | 28222186|28877472|30111836 |
Tgene | NAA15 | GO:0006474 | N-terminal protein amino acid acetylation | 15496142 |
Tgene | NAA15 | GO:0045893 | positive regulation of transcription, DNA-templated | 12145306 |
![]() Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr4:113362261/chr4:140291365) - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. - How to search 1. Put your fusion gene symbol. 2. Press the tab key until there will be shown the breakpoint information filled. 4. Go down and press 'Search' tab twice. 4. Go down to have the hyperlink of the search result. 5. Click the hyperlink. 6. See the FGviewer result for your fusion gene. |
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![]() * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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![]() * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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Fusion Amino Acid Sequences |
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Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
ENST00000458497 | ALPK1 | chr4 | 113362261 | - | ENST00000296543 | NAA15 | chr4 | 140291365 | + | 8152 | 4006 | 279 | 4853 | 1524 |
ENST00000458497 | ALPK1 | chr4 | 113362261 | - | ENST00000398947 | NAA15 | chr4 | 140291365 | + | 8135 | 4006 | 279 | 4850 | 1523 |
ENST00000177648 | ALPK1 | chr4 | 113362261 | - | ENST00000296543 | NAA15 | chr4 | 140291365 | + | 8073 | 3927 | 125 | 4774 | 1549 |
ENST00000177648 | ALPK1 | chr4 | 113362261 | - | ENST00000398947 | NAA15 | chr4 | 140291365 | + | 8056 | 3927 | 125 | 4771 | 1548 |
ENST00000504176 | ALPK1 | chr4 | 113362261 | - | ENST00000296543 | NAA15 | chr4 | 140291365 | + | 7908 | 3762 | 212 | 4609 | 1465 |
ENST00000504176 | ALPK1 | chr4 | 113362261 | - | ENST00000398947 | NAA15 | chr4 | 140291365 | + | 7891 | 3762 | 212 | 4606 | 1464 |
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Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
ENST00000458497 | ENST00000296543 | ALPK1 | chr4 | 113362261 | - | NAA15 | chr4 | 140291365 | + | 0.000201704 | 0.99979836 |
ENST00000458497 | ENST00000398947 | ALPK1 | chr4 | 113362261 | - | NAA15 | chr4 | 140291365 | + | 0.000180267 | 0.9998198 |
ENST00000177648 | ENST00000296543 | ALPK1 | chr4 | 113362261 | - | NAA15 | chr4 | 140291365 | + | 0.000186645 | 0.9998134 |
ENST00000177648 | ENST00000398947 | ALPK1 | chr4 | 113362261 | - | NAA15 | chr4 | 140291365 | + | 0.000166989 | 0.999833 |
ENST00000504176 | ENST00000296543 | ALPK1 | chr4 | 113362261 | - | NAA15 | chr4 | 140291365 | + | 0.000197065 | 0.999803 |
ENST00000504176 | ENST00000398947 | ALPK1 | chr4 | 113362261 | - | NAA15 | chr4 | 140291365 | + | 0.000175144 | 0.99982494 |
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Get the fusion protein sequences from here. |
Fusion protein sequence information is available in the fasta format. >FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP |
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Fusion Protein Breakpoint Sequences for ALPK1-NAA15 |
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Hgene | Hchr | Hbp | Tgene | Tchr | Tbp | Length(fusion protein) | BP in fusion protein | Peptide |
ALPK1 | chr4 | 113362261 | NAA15 | chr4 | 140291365 | 3762 | 1184 | RLSLTRPSMEKPSNMSDKELKKLRNK |
ALPK1 | chr4 | 113362261 | NAA15 | chr4 | 140291365 | 3927 | 1268 | RLSLTRPSMEKPSNMSDKELKKLRNK |
ALPK1 | chr4 | 113362261 | NAA15 | chr4 | 140291365 | 4006 | 1243 | RLSLTRPSMEKPSNMSDKELKKLRNK |
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Potential FusionNeoAntigen Information of ALPK1-NAA15 in HLA I |
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ALPK1-NAA15_113362261_140291365.msa |
![]() * We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5) |
Fusion gene | Hchr | Hbp | Tgene | Tchr | Tbp | HLA I | FusionNeoAntigen peptide | Binding score | Immunogenic score | Neoantigen start (at BP 13) | Neoantigen end (at BP 13) |
ALPK1-NAA15 | chr4 | 113362261 | chr4 | 140291365 | 3927 | HLA-B07:12 | RPSMEKPSNM | 0.9982 | 0.52 | 5 | 15 |
ALPK1-NAA15 | chr4 | 113362261 | chr4 | 140291365 | 3927 | HLA-B07:12 | KPSNMSDKEL | 0.9497 | 0.6119 | 10 | 20 |
ALPK1-NAA15 | chr4 | 113362261 | chr4 | 140291365 | 3927 | HLA-B42:02 | RPSMEKPSNM | 0.9126 | 0.5193 | 5 | 15 |
ALPK1-NAA15 | chr4 | 113362261 | chr4 | 140291365 | 3927 | HLA-B42:01 | RPSMEKPSNM | 0.8831 | 0.5111 | 5 | 15 |
ALPK1-NAA15 | chr4 | 113362261 | chr4 | 140291365 | 3927 | HLA-B42:01 | KPSNMSDKEL | 0.5099 | 0.6 | 10 | 20 |
ALPK1-NAA15 | chr4 | 113362261 | chr4 | 140291365 | 3927 | HLA-B08:12 | RPSMEKPSNM | 0.8265 | 0.6767 | 5 | 15 |
ALPK1-NAA15 | chr4 | 113362261 | chr4 | 140291365 | 3927 | HLA-B67:01 | RPSMEKPSNM | 0.5386 | 0.7963 | 5 | 15 |
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Potential FusionNeoAntigen Information of ALPK1-NAA15 in HLA II |
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![]() * We used NetMHCIIpan v4.1 (%rank<0.5). |
Fusion gene | Hchr | Hbp | Tgene | Tchr | Tbp | HLA II | FusionNeoAntigen peptide | Neoantigen start (at BP 13) | Neoantigen end (at BP 13) |
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Fusion breakpoint peptide structures of ALPK1-NAA15 |
![]() * The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA. |
File name | BPseq | Hgene | Tgene | Hchr | Hbp | Tchr | Tbp | AAlen |
7001 | PSMEKPSNMSDKEL | ALPK1 | NAA15 | chr4 | 113362261 | chr4 | 140291365 | 3927 |
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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of ALPK1-NAA15 |
![]() * We used Glide to predict the interaction between HLAs and neoantigens. |
HLA allele | PDB ID | File name | BPseq | Docking score | Glide score |
HLA-B14:02 | 3BVN | 7001 | PSMEKPSNMSDKEL | -7.15543 | -7.26883 |
HLA-B14:02 | 3BVN | 7001 | PSMEKPSNMSDKEL | -4.77435 | -5.80965 |
HLA-B52:01 | 3W39 | 7001 | PSMEKPSNMSDKEL | -6.80875 | -6.92215 |
HLA-B52:01 | 3W39 | 7001 | PSMEKPSNMSDKEL | -4.20386 | -5.23916 |
HLA-A11:01 | 4UQ2 | 7001 | PSMEKPSNMSDKEL | -7.5194 | -8.5547 |
HLA-A11:01 | 4UQ2 | 7001 | PSMEKPSNMSDKEL | -6.9601 | -7.0735 |
HLA-A24:02 | 5HGA | 7001 | PSMEKPSNMSDKEL | -7.52403 | -7.63743 |
HLA-A24:02 | 5HGA | 7001 | PSMEKPSNMSDKEL | -5.82433 | -6.85963 |
HLA-B27:05 | 6PYJ | 7001 | PSMEKPSNMSDKEL | -3.28285 | -4.31815 |
HLA-B44:05 | 3DX8 | 7001 | PSMEKPSNMSDKEL | -5.91172 | -6.94702 |
HLA-B44:05 | 3DX8 | 7001 | PSMEKPSNMSDKEL | -4.24346 | -4.35686 |
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Vaccine Design for the FusionNeoAntigens of ALPK1-NAA15 |
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Fusion gene | Hchr | Hbp | Tchr | Tbp | Start in +/-13AA | End in +/-13AA | FusionNeoAntigen peptide sequence | FusionNeoAntigen RNA sequence |
ALPK1-NAA15 | chr4 | 113362261 | chr4 | 140291365 | 10 | 20 | KPSNMSDKEL | AGAAACCATCAAACATGTCTGACAAAGAGC |
ALPK1-NAA15 | chr4 | 113362261 | chr4 | 140291365 | 5 | 15 | RPSMEKPSNM | CTAGACCTTCAATGGAGAAACCATCAAACA |
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Fusion gene | Hchr | Hbp | Tchr | Tbp | Start in +/-13AA | End in +/-13AA | FusionNeoAntigen peptide | FusionNEoAntigen RNA sequence |
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Information of the samples that have these potential fusion neoantigens of ALPK1-NAA15 |
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Cancer type | Fusion gene | Hchr | Hbp | Henst | Tchr | Tbp | Tenst | Sample |
SKCM | ALPK1-NAA15 | chr4 | 113362261 | ENST00000177648 | chr4 | 140291365 | ENST00000296543 | TCGA-W3-A825-06A |
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Potential target of CAR-T therapy development for ALPK1-NAA15 |
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![]() * Minus value of BPloci means that the break point is located before the CDS. |
- In-frame and retained 'Transmembrane'. |
Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
![]() * We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image. |
Hgene | Hchr | Hbp | Henst | Tgene | Tchr | Tbp | Tenst | DeepLoc result |
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Related Drugs to ALPK1-NAA15 |
![]() (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
Hgene | Tgene | Drug | Source | PMID |
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Related Diseases to ALPK1-NAA15 |
![]() (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
Hgene | Tgene | Disease | Source | PMID |
![]() (DisGeNet 4.0) |
Partner | Gene | Disease ID | Disease name | # pubmeds | Source |