![]() |
|||||||
|
Fusion Protein:KMT2B-CEACAM16 |
Fusion Gene and Fusion Protein Summary |
![]() |
Fusion partner gene information | Fusion gene name: KMT2B-CEACAM16 | FusionPDB ID: 43227 | FusionGDB2.0 ID: 54219 | Hgene | Tgene | Gene symbol | KMT2B | CEACAM16 | Gene ID | 9757 | 388551 |
Gene name | lysine methyltransferase 2B | CEA cell adhesion molecule 16, tectorial membrane component | |
Synonyms | CXXC10|DYT28|HRX2|MLL1B|MLL2|MLL4|TRX2|WBP-7|WBP7 | CEAL2|DFNA4B|DFNB113 | |
Cytomap | 19q13.12 | 19q13.31-q13.32 | |
Type of gene | protein-coding | protein-coding | |
Description | histone-lysine N-methyltransferase 2BWW domain binding protein 7histone-lysine N-methyltransferase MLL4lysine (K)-specific methyltransferase 2Bmixed lineage leukemia gene homolog 2myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosoph | carcinoembryonic antigen-related cell adhesion molecule 16carcinoembryonic antigen like-2 proteincarcinoembryonic antigen related cell adhesion molecule 16 | |
Modification date | 20200313 | 20200313 | |
UniProtAcc | Q9UMN6 Main function of 5'-partner protein: FUNCTION: Histone methyltransferase that methylates 'Lys-4' of histone H3 (PubMed:17707229). H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation (PubMed:17707229). Plays a central role in beta-globin locus transcription regulation by being recruited by NFE2 (PubMed:17707229). Plays an important role in controlling bulk H3K4me during oocyte growth and preimplantation development (By similarity). Required during the transcriptionally active period of oocyte growth for the establishment and/or maintenance of bulk H3K4 trimethylation (H3K4me3), global transcriptional silencing that preceeds resumption of meiosis, oocyte survival and normal zygotic genome activation (By similarity). {ECO:0000250|UniProtKB:O08550, ECO:0000269|PubMed:17707229}. | . | |
Ensembl transtripts involved in fusion gene | ENST ids | ENST00000607650, ENST00000222270, ENST00000420124, ENST00000341701, | ENST00000405314, ENST00000587331, |
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0) | * DoF score | 6 X 6 X 4=144 | 6 X 5 X 6=180 |
# samples | 5 | 7 | |
** MAII score | log2(5/144*10)=-1.52606881166759 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | log2(7/180*10)=-1.36257007938471 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | |
Fusion gene context | PubMed: KMT2B [Title/Abstract] AND CEACAM16 [Title/Abstract] AND fusion [Title/Abstract] | ||
Fusion neoantigen context | PubMed: KMT2B [Title/Abstract] AND CEACAM16 [Title/Abstract] AND neoantigen [Title/Abstract] | ||
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0) | KMT2B(36228164)-CEACAM16(45213768), # samples:1 | ||
Anticipated loss of major functional domain due to fusion event. | KMT2B-CEACAM16 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. KMT2B-CEACAM16 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. KMT2B-CEACAM16 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. KMT2B-CEACAM16 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. |
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
![]() |
Partner | Gene | GO ID | GO term | PubMed ID |
Hgene | KMT2B | GO:0044648 | histone H3-K4 dimethylation | 25561738 |
Hgene | KMT2B | GO:0097692 | histone H3-K4 monomethylation | 25561738 |
![]() Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr19:36228164/chr19:45213768) - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. - How to search 1. Put your fusion gene symbol. 2. Press the tab key until there will be shown the breakpoint information filled. 4. Go down and press 'Search' tab twice. 4. Go down to have the hyperlink of the search result. 5. Click the hyperlink. 6. See the FGviewer result for your fusion gene. |
![]() |
![]() |
![]() * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
![]() |
![]() * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
![]() |
Top |
Fusion Amino Acid Sequences |
![]() |
Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
ENST00000222270 | KMT2B | chr19 | 36228164 | + | ENST00000587331 | CEACAM16 | chr19 | 45213768 | + | 7769 | 7550 | 0 | 7568 | 2522 |
ENST00000222270 | KMT2B | chr19 | 36228164 | + | ENST00000405314 | CEACAM16 | chr19 | 45213768 | + | 7769 | 7550 | 0 | 7568 | 2522 |
ENST00000420124 | KMT2B | chr19 | 36228164 | + | ENST00000587331 | CEACAM16 | chr19 | 45213768 | + | 7769 | 7550 | 0 | 7568 | 2522 |
ENST00000420124 | KMT2B | chr19 | 36228164 | + | ENST00000405314 | CEACAM16 | chr19 | 45213768 | + | 7769 | 7550 | 0 | 7568 | 2522 |
![]() |
Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
ENST00000222270 | ENST00000587331 | KMT2B | chr19 | 36228164 | + | CEACAM16 | chr19 | 45213768 | + | 0.005280212 | 0.99471974 |
ENST00000222270 | ENST00000405314 | KMT2B | chr19 | 36228164 | + | CEACAM16 | chr19 | 45213768 | + | 0.005280212 | 0.99471974 |
ENST00000420124 | ENST00000587331 | KMT2B | chr19 | 36228164 | + | CEACAM16 | chr19 | 45213768 | + | 0.005280212 | 0.99471974 |
ENST00000420124 | ENST00000405314 | KMT2B | chr19 | 36228164 | + | CEACAM16 | chr19 | 45213768 | + | 0.005280212 | 0.99471974 |
![]() |
Get the fusion protein sequences from here. |
Fusion protein sequence information is available in the fasta format. >FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP |
Top |
Fusion Protein Breakpoint Sequences for KMT2B-CEACAM16 |
![]() |
Hgene | Hchr | Hbp | Tgene | Tchr | Tbp | Length(fusion protein) | BP in fusion protein | Peptide |
KMT2B | chr19 | 36228164 | CEACAM16 | chr19 | 45213768 | 7550 | 20 | GSCPGPGSARGRFPGRPRGAGGGGGR |
KMT2B | chr19 | 36228164 | CEACAM16 | chr19 | 45213768 | 7550 | 24 | GPGSARGRFPGRPRGAGGGGGRGGRG |
Top |
Potential FusionNeoAntigen Information of KMT2B-CEACAM16 in HLA I |
![]() |
KMT2B-CEACAM16_36228164_45213768.msa |
![]() * We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5) |
Fusion gene | Hchr | Hbp | Tgene | Tchr | Tbp | HLA I | FusionNeoAntigen peptide | Binding score | Immunogenic score | Neoantigen start (at BP 13) | Neoantigen end (at BP 13) |
KMT2B-CEACAM16 | chr19 | 36228164 | chr19 | 45213768 | 7550 | HLA-A31:02 | RGRFPGRPR | 0.8076 | 0.6026 | 9 | 18 |
KMT2B-CEACAM16 | chr19 | 36228164 | chr19 | 45213768 | 7550 | HLA-A31:02 | SARGRFPGR | 0.8052 | 0.5641 | 7 | 16 |
KMT2B-CEACAM16 | chr19 | 36228164 | chr19 | 45213768 | 7550 | HLA-B27:04 | GRFPGRPRGA | 0.9995 | 0.7844 | 10 | 20 |
KMT2B-CEACAM16 | chr19 | 36228164 | chr19 | 45213768 | 7550 | HLA-B27:14 | GRFPGRPRG | 0.9958 | 0.6921 | 10 | 19 |
KMT2B-CEACAM16 | chr19 | 36228164 | chr19 | 45213768 | 7550 | HLA-A31:01 | RGRFPGRPR | 0.9772 | 0.5709 | 9 | 18 |
KMT2B-CEACAM16 | chr19 | 36228164 | chr19 | 45213768 | 7550 | HLA-A31:01 | SARGRFPGR | 0.9698 | 0.5374 | 7 | 16 |
KMT2B-CEACAM16 | chr19 | 36228164 | chr19 | 45213768 | 7550 | HLA-B27:14 | GRFPGRPRGA | 0.9997 | 0.7406 | 10 | 20 |
KMT2B-CEACAM16 | chr19 | 36228164 | chr19 | 45213768 | 7550 | HLA-B73:01 | GRFPGRPRGA | 0.9956 | 0.8181 | 10 | 20 |
KMT2B-CEACAM16 | chr19 | 36228164 | chr19 | 45213768 | 7550 | HLA-A30:01 | RGRFPGRPR | 0.99 | 0.8346 | 9 | 18 |
KMT2B-CEACAM16 | chr19 | 36228164 | chr19 | 45213768 | 7550 | HLA-A30:01 | SARGRFPGR | 0.9863 | 0.8659 | 7 | 16 |
KMT2B-CEACAM16 | chr19 | 36228164 | chr19 | 45213768 | 7550 | HLA-B27:10 | GRFPGRPRGA | 0.9995 | 0.8733 | 10 | 20 |
Top |
Potential FusionNeoAntigen Information of KMT2B-CEACAM16 in HLA II |
![]() |
![]() * We used NetMHCIIpan v4.1 (%rank<0.5). |
Fusion gene | Hchr | Hbp | Tgene | Tchr | Tbp | HLA II | FusionNeoAntigen peptide | Neoantigen start (at BP 13) | Neoantigen end (at BP 13) |
Top |
Fusion breakpoint peptide structures of KMT2B-CEACAM16 |
![]() * The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA. |
File name | BPseq | Hgene | Tgene | Hchr | Hbp | Tchr | Tbp | AAlen |
3110 | GSARGRFPGRPRGA | KMT2B | CEACAM16 | chr19 | 36228164 | chr19 | 45213768 | 7550 |
Top |
Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of KMT2B-CEACAM16 |
![]() * We used Glide to predict the interaction between HLAs and neoantigens. |
HLA allele | PDB ID | File name | BPseq | Docking score | Glide score |
HLA-B14:02 | 3BVN | 3110 | GSARGRFPGRPRGA | -7.15543 | -7.26883 |
HLA-B14:02 | 3BVN | 3110 | GSARGRFPGRPRGA | -4.77435 | -5.80965 |
HLA-B52:01 | 3W39 | 3110 | GSARGRFPGRPRGA | -6.80875 | -6.92215 |
HLA-B52:01 | 3W39 | 3110 | GSARGRFPGRPRGA | -4.20386 | -5.23916 |
HLA-A11:01 | 4UQ2 | 3110 | GSARGRFPGRPRGA | -7.5194 | -8.5547 |
HLA-A11:01 | 4UQ2 | 3110 | GSARGRFPGRPRGA | -6.9601 | -7.0735 |
HLA-A24:02 | 5HGA | 3110 | GSARGRFPGRPRGA | -7.52403 | -7.63743 |
HLA-A24:02 | 5HGA | 3110 | GSARGRFPGRPRGA | -5.82433 | -6.85963 |
HLA-B27:05 | 6PYJ | 3110 | GSARGRFPGRPRGA | -3.28285 | -4.31815 |
HLA-B44:05 | 3DX8 | 3110 | GSARGRFPGRPRGA | -5.91172 | -6.94702 |
HLA-B44:05 | 3DX8 | 3110 | GSARGRFPGRPRGA | -4.24346 | -4.35686 |
Top |
Vaccine Design for the FusionNeoAntigens of KMT2B-CEACAM16 |
![]() |
Fusion gene | Hchr | Hbp | Tchr | Tbp | Start in +/-13AA | End in +/-13AA | FusionNeoAntigen peptide sequence | FusionNeoAntigen RNA sequence |
KMT2B-CEACAM16 | chr19 | 36228164 | chr19 | 45213768 | 10 | 19 | GRFPGRPRG | CTATCTCCGCCCTGGGGTAACAGCGTG |
KMT2B-CEACAM16 | chr19 | 36228164 | chr19 | 45213768 | 10 | 20 | GRFPGRPRGA | CTATCTCCGCCCTGGGGTAACAGCGTGACC |
KMT2B-CEACAM16 | chr19 | 36228164 | chr19 | 45213768 | 7 | 16 | SARGRFPGR | GGCAGAGGTCTATCTCCGCCCTGGGGT |
KMT2B-CEACAM16 | chr19 | 36228164 | chr19 | 45213768 | 9 | 18 | RGRFPGRPR | GGTCTATCTCCGCCCTGGGGTAACAGC |
![]() |
Fusion gene | Hchr | Hbp | Tchr | Tbp | Start in +/-13AA | End in +/-13AA | FusionNeoAntigen peptide | FusionNEoAntigen RNA sequence |
Top |
Information of the samples that have these potential fusion neoantigens of KMT2B-CEACAM16 |
![]() |
Cancer type | Fusion gene | Hchr | Hbp | Henst | Tchr | Tbp | Tenst | Sample |
LUAD | KMT2B-CEACAM16 | chr19 | 36228164 | ENST00000222270 | chr19 | 45213768 | ENST00000405314 | TCGA-49-4488-01A |
Top |
Potential target of CAR-T therapy development for KMT2B-CEACAM16 |
![]() |
![]() * Minus value of BPloci means that the break point is located before the CDS. |
- In-frame and retained 'Transmembrane'. |
Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
![]() * We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image. |
Hgene | Hchr | Hbp | Henst | Tgene | Tchr | Tbp | Tenst | DeepLoc result |
Top |
Related Drugs to KMT2B-CEACAM16 |
![]() (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
Hgene | Tgene | Drug | Source | PMID |
Top |
Related Diseases to KMT2B-CEACAM16 |
![]() (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
Hgene | Tgene | Disease | Source | PMID |
![]() (DisGeNet 4.0) |
Partner | Gene | Disease ID | Disease name | # pubmeds | Source |