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Center for Computational Systems Medicine
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Fusion Gene and Fusion Protein Summary

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Fusion Amino Acid Sequences (multiple BPs and multiple gene isoforms)

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Fusion Protein Breakpoint Sequences - (for the Screening of the FusionNeoAntigens)

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Potential FusionNeoAntigens in HLA I - (netMHCpan v4.1 + deepHLApan v1.1)

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Potential FusionNeoAntigens in HLA II - (netMHCIIpan v4.1)

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Fusion Breakpoint 14 AA Peptide Structure - (RoseTTAFold)

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D - (Glide)

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Vaccine Design for the FusionNeoAntigens (RNA/protein sequences)

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Potential target of CAR-T therapy development

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Information on the samples that have these potential fusion neoantigens

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Fusion Protein Targeting Drugs - (Manual Curation)

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Fusion Protein Related diseases - (Manual Curation)

Fusion Protein:MIEF2-ANKRD30A

Fusion Gene and Fusion Protein Summary

check button Fusion gene summary
Fusion partner gene informationFusion gene name: MIEF2-ANKRD30A
FusionPDB ID: 53615
FusionGDB2.0 ID: 53615
HgeneTgene
Gene symbol

MIEF2

ANKRD30A

Gene ID

125170

91074

Gene namemitochondrial elongation factor 2ankyrin repeat domain 30A
SynonymsMID49|SMCR7NY-BR-1
Cytomap

17p11.2

10p11.21

Type of geneprotein-codingprotein-coding
Descriptionmitochondrial dynamics protein MID49Smith-Magenis syndrome chromosomal region candidate gene 7 proteinSmith-Magenis syndrome chromosome region, candidate 7mitochondrial dynamic protein MID49mitochondrial dynamic protein of 49 kDamitochondrial dynamicankyrin repeat domain-containing protein 30Aserologically defined breast cancer antigen NY-BR-1
Modification date2020032720200313
UniProtAcc

Q96C03

Main function of 5'-partner protein: FUNCTION: Mitochondrial outer membrane protein which regulates mitochondrial organization (PubMed:29361167). It is required for mitochondrial fission and promotes the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface independently of the mitochondrial fission FIS1 and MFF proteins. Regulates DNM1L GTPase activity. {ECO:0000269|PubMed:21508961, ECO:0000269|PubMed:23283981, ECO:0000269|PubMed:23530241, ECO:0000269|PubMed:23921378, ECO:0000269|PubMed:29361167, ECO:0000269|PubMed:29899447}.

Q9BXX3

Main function of 5'-partner protein:
Ensembl transtripts involved in fusion geneENST idsENST00000577216, ENST00000323019, 
ENST00000395703, ENST00000395704, 
ENST00000395706, ENST00000578174, 
ENST00000578621, 		ENST00000361713, 
ENST00000374660, ENST00000602533, 
ENST00000475522, 
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)* DoF score2 X 2 X 1=47 X 7 X 2=98
# samples 17
** MAII scorelog2(1/4*10)=1.32192809488736log2(7/98*10)=-0.485426827170242
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Fusion gene context

PubMed: MIEF2 [Title/Abstract] AND ANKRD30A [Title/Abstract] AND fusion [Title/Abstract]

Fusion neoantigen context

PubMed: MIEF2 [Title/Abstract] AND ANKRD30A [Title/Abstract] AND neoantigen [Title/Abstract]

Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)MIEF2(18166562)-ANKRD30A(37440988), # samples:2
Anticipated loss of major functional domain due to fusion event.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgeneMIEF2

GO:0003374

dynamin family protein polymerization involved in mitochondrial fission

23530241

HgeneMIEF2

GO:0007005

mitochondrion organization

21508961

HgeneMIEF2

GO:0090141

positive regulation of mitochondrial fission

23283981|23530241

HgeneMIEF2

GO:0090314

positive regulation of protein targeting to membrane

23283981

HgeneMIEF2

GO:0090314

positive regulation of protein targeting to membrane

23921378



check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr17:18166562/chr10:37440988)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonRetention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here.

check buttonFusion gene breakpoints across MIEF2 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check buttonFusion gene breakpoints across ANKRD30A (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure


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Fusion Amino Acid Sequences


check buttonFusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000323019MIEF2chr1718166562+ENST00000374660ANKRD30Achr1037440988+37075211934261135
ENST00000323019MIEF2chr1718166562+ENST00000361713ANKRD30Achr1037440988+33505211930691016
ENST00000323019MIEF2chr1718166562+ENST00000602533ANKRD30Achr1037440988+34035211930691016
ENST00000578174MIEF2chr1718166562+ENST00000374660ANKRD30Achr1037440988+36444587933631094
ENST00000578174MIEF2chr1718166562+ENST00000361713ANKRD30Achr1037440988+3287458793006975
ENST00000578174MIEF2chr1718166562+ENST00000602533ANKRD30Achr1037440988+3340458793006975
ENST00000395704MIEF2chr1718166562+ENST00000374660ANKRD30Achr1037440988+36444587933631094
ENST00000395704MIEF2chr1718166562+ENST00000361713ANKRD30Achr1037440988+3287458793006975
ENST00000395704MIEF2chr1718166562+ENST00000602533ANKRD30Achr1037440988+3340458793006975
ENST00000395703MIEF2chr1718166562+ENST00000374660ANKRD30Achr1037440988+37415555303460976
ENST00000395703MIEF2chr1718166562+ENST00000361713ANKRD30Achr1037440988+33845555303103857
ENST00000395703MIEF2chr1718166562+ENST00000602533ANKRD30Achr1037440988+34375555303103857
ENST00000578621MIEF2chr1718166562+ENST00000374660ANKRD30Achr1037440988+35964103133151094
ENST00000578621MIEF2chr1718166562+ENST00000361713ANKRD30Achr1037440988+3239410312958975
ENST00000578621MIEF2chr1718166562+ENST00000602533ANKRD30Achr1037440988+3292410312958975
ENST00000395706MIEF2chr1718166562+ENST00000374660ANKRD30Achr1037440988+35553692632741082
ENST00000395706MIEF2chr1718166562+ENST00000361713ANKRD30Achr1037440988+3198369262917963
ENST00000395706MIEF2chr1718166562+ENST00000602533ANKRD30Achr1037440988+3251369262917963

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000323019ENST00000374660MIEF2chr1718166562+ANKRD30Achr1037440988+0.0013257020.9986743
ENST00000323019ENST00000361713MIEF2chr1718166562+ANKRD30Achr1037440988+0.0015785020.99842155
ENST00000323019ENST00000602533MIEF2chr1718166562+ANKRD30Achr1037440988+0.0016350480.998365
ENST00000578174ENST00000374660MIEF2chr1718166562+ANKRD30Achr1037440988+0.0013536650.9986463
ENST00000578174ENST00000361713MIEF2chr1718166562+ANKRD30Achr1037440988+0.001724880.99827516
ENST00000578174ENST00000602533MIEF2chr1718166562+ANKRD30Achr1037440988+0.001782880.99821705
ENST00000395704ENST00000374660MIEF2chr1718166562+ANKRD30Achr1037440988+0.0013536650.9986463
ENST00000395704ENST00000361713MIEF2chr1718166562+ANKRD30Achr1037440988+0.001724880.99827516
ENST00000395704ENST00000602533MIEF2chr1718166562+ANKRD30Achr1037440988+0.001782880.99821705
ENST00000395703ENST00000374660MIEF2chr1718166562+ANKRD30Achr1037440988+0.0031239540.996876
ENST00000395703ENST00000361713MIEF2chr1718166562+ANKRD30Achr1037440988+0.0038455490.9961545
ENST00000395703ENST00000602533MIEF2chr1718166562+ANKRD30Achr1037440988+0.0039355360.9960645
ENST00000578621ENST00000374660MIEF2chr1718166562+ANKRD30Achr1037440988+0.0014367760.99856323
ENST00000578621ENST00000361713MIEF2chr1718166562+ANKRD30Achr1037440988+0.0019234620.99807656
ENST00000578621ENST00000602533MIEF2chr1718166562+ANKRD30Achr1037440988+0.001972510.9980275
ENST00000395706ENST00000374660MIEF2chr1718166562+ANKRD30Achr1037440988+0.0014856030.99851435
ENST00000395706ENST00000361713MIEF2chr1718166562+ANKRD30Achr1037440988+0.0020547070.99794525
ENST00000395706ENST00000602533MIEF2chr1718166562+ANKRD30Achr1037440988+0.0021162570.99788374

check button Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones.

Get the fusion protein sequences from here.

Fusion protein sequence information is available in the fasta format.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

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Fusion Protein Breakpoint Sequences for MIEF2-ANKRD30A

check button +/-13 AA sequence from the breakpoints of the fusion protein sequences.
HgeneHchrHbpTgeneTchrTbpLength(fusion protein)BP in fusion proteinPeptide
MIEF2chr1718166562ANKRD30Achr1037440988369114QPVLPLAPSSSAPDPMFPPESKQKDY
MIEF2chr1718166562ANKRD30Achr1037440988369209FKAEPPGKPSAFEPATEMQKSVPNKA
MIEF2chr1718166562ANKRD30Achr1037440988369328FKAEPPEKPSAFEPAIEMQKSVPNKA
MIEF2chr1718166562ANKRD30Achr1037440988369447FKAEPPEKPSAFEPAIEMQKSVPNKA
MIEF2chr1718166562ANKRD30Achr1037440988369566FKAEPPEKPSAFEPAIEMQKSVPNKA
MIEF2chr1718166562ANKRD30Achr1037440988410126QPVLPLAPSSSAPDPMFPPESKQKDY
MIEF2chr1718166562ANKRD30Achr1037440988410221FKAEPPGKPSAFEPATEMQKSVPNKA
MIEF2chr1718166562ANKRD30Achr1037440988410340FKAEPPEKPSAFEPAIEMQKSVPNKA
MIEF2chr1718166562ANKRD30Achr1037440988410459FKAEPPEKPSAFEPAIEMQKSVPNKA
MIEF2chr1718166562ANKRD30Achr1037440988410578FKAEPPEKPSAFEPAIEMQKSVPNKA
MIEF2chr1718166562ANKRD30Achr1037440988458126QPVLPLAPSSSAPDPMFPPESKQKDY
MIEF2chr1718166562ANKRD30Achr1037440988458221FKAEPPGKPSAFEPATEMQKSVPNKA
MIEF2chr1718166562ANKRD30Achr1037440988458340FKAEPPEKPSAFEPAIEMQKSVPNKA
MIEF2chr1718166562ANKRD30Achr1037440988458459FKAEPPEKPSAFEPAIEMQKSVPNKA
MIEF2chr1718166562ANKRD30Achr1037440988458578FKAEPPEKPSAFEPAIEMQKSVPNKA
MIEF2chr1718166562ANKRD30Achr1037440988521167QPVLPLAPSSSAPDPMFPPESKQKDY
MIEF2chr1718166562ANKRD30Achr1037440988521262FKAEPPGKPSAFEPATEMQKSVPNKA
MIEF2chr1718166562ANKRD30Achr1037440988521381FKAEPPEKPSAFEPAIEMQKSVPNKA
MIEF2chr1718166562ANKRD30Achr1037440988521500FKAEPPEKPSAFEPAIEMQKSVPNKA
MIEF2chr1718166562ANKRD30Achr1037440988521619FKAEPPEKPSAFEPAIEMQKSVPNKA
MIEF2chr1718166562ANKRD30Achr1037440988555103FKAEPPGKPSAFEPATEMQKSVPNKA
MIEF2chr1718166562ANKRD30Achr1037440988555222FKAEPPEKPSAFEPAIEMQKSVPNKA
MIEF2chr1718166562ANKRD30Achr1037440988555341FKAEPPEKPSAFEPAIEMQKSVPNKA
MIEF2chr1718166562ANKRD30Achr1037440988555460FKAEPPEKPSAFEPAIEMQKSVPNKA

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Potential FusionNeoAntigen Information of MIEF2-ANKRD30A in HLA I

check button Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.
MIEF2-ANKRD30A_18166562_37440988.msa

check button Potential FusionNeoAntigen Information
* We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5)
Fusion geneHchrHbpTgeneTchrTbpHLA IFusionNeoAntigen peptideBinding scoreImmunogenic scoreNeoantigen start (at BP 13)Neoantigen end (at BP 13)
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B35:08SAFEPAIEM0.99920.882918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B35:01SAFEPAIEM0.99780.8945918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B15:02SAFEPAIEM0.99750.9681918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B15:25SAFEPAIEM0.9970.9642918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B35:05SAFEPAIEM0.99450.6858918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B15:17SAFEPAIEM0.9940.9448918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B35:03SAFEPAIEM0.99170.9079918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B15:03SAFEPAIEM0.98930.8033918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B15:16SAFEPAIEM0.98650.7541918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B15:18SAFEPAIEM0.98220.7779918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B14:01SAFEPAIEM0.96230.8952918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B14:02SAFEPAIEM0.96230.8952918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B35:04SAFEPAIEM0.9440.9724918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B35:02SAFEPAIEM0.9440.9724918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B52:01SAFEPAIEM0.9090.9748918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B51:01SAFEPAIEM0.88920.5075918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B15:10SAFEPAIEM0.88910.6757918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B15:37SAFEPAIEM0.85970.7273918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B35:02KPSAFEPAI0.83880.9414716
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B35:04KPSAFEPAI0.83880.9414716
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B35:03KPSAFEPAI0.83750.8707716
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-A02:21SAFEPAIEM0.70850.7549918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B13:01SAFEPAIEM0.57850.9551918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B39:13SAFEPAIEM0.45540.967918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B35:05KPSAFEPAIEM0.99350.5959718
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B35:03KPSAFEPAIEM0.97990.88718
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B35:04KPSAFEPAIEM0.9390.9599718
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B35:02KPSAFEPAIEM0.9390.9599718
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C04:10AFEPAIEM0.99980.77471018
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C04:07AFEPAIEM0.99980.80791018
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C04:14AFEPAIEM0.9850.7861018
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C03:19SAFEPAIEM0.99960.9955918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C03:08SAFEPAIEM0.99960.9304918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C03:07SAFEPAIEM0.9990.9815918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C12:16SAFEPAIEM0.99890.9783918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C15:04SAFEPAIEM0.99890.8879918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C15:06SAFEPAIEM0.99860.937918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B15:21SAFEPAIEM0.99790.9141918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B15:05SAFEPAIEM0.99740.8785918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B15:31SAFEPAIEM0.99720.8889918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C04:06SAFEPAIEM0.9950.9562918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C08:04SAFEPAIEM0.99070.9673918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C08:13SAFEPAIEM0.99070.9673918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C12:12SAFEPAIEM0.98580.9548918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C03:14SAFEPAIEM0.98160.9853918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C04:14SAFEPAIEM0.97720.9024918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B14:03SAFEPAIEM0.97640.8754918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C06:03SAFEPAIEM0.96750.9961918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C12:04SAFEPAIEM0.96640.9957918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C02:06SAFEPAIEM0.96280.9618918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C08:03SAFEPAIEM0.96150.9907918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C07:10SAFEPAIEM0.95740.9829918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C07:13SAFEPAIEM0.95330.9667918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C07:05SAFEPAIEM0.95120.9831918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C07:29SAFEPAIEM0.94610.9537918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B35:12SAFEPAIEM0.9440.9724918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C07:67SAFEPAIEM0.93130.96918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C07:80SAFEPAIEM0.93130.96918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C01:30SAFEPAIEM0.93090.9675918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C07:27SAFEPAIEM0.92660.9675918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C07:95SAFEPAIEM0.91510.8488918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C04:07SAFEPAIEM0.91010.9087918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B15:04SAFEPAIEM0.910.9495918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C07:19SAFEPAIEM0.90260.8544918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B51:07SAFEPAIEM0.89870.9606918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C05:09SAFEPAIEM0.89030.9588918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B78:01SAFEPAIEM0.88090.5462918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C07:46SAFEPAIEM0.8780.9271918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B35:12KPSAFEPAI0.83880.9414716
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B39:09SAFEPAIEM0.76910.7635918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C01:17SAFEPAIEM0.76370.9522918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B39:10SAFEPAIEM0.75310.9564918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B42:02KPSAFEPAI0.57740.6559716
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C08:15SAFEPAIEM0.56060.9799918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B42:01KPSAFEPAI0.55840.6501716
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B39:10KPSAFEPAI0.48190.9294716
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B07:12KPSAFEPAIEM0.99980.5228718
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B42:02KPSAFEPAIEM0.96720.67718
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B39:10KPSAFEPAIEM0.95470.9362718
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B35:12KPSAFEPAIEM0.9390.9599718
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B42:01KPSAFEPAIEM0.93890.665718
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C18:01AFEPAIEM0.99980.84231018
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C04:01AFEPAIEM0.99980.80791018
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C04:04AFEPAIEM0.99380.93281018
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C14:02AFEPAIEM0.98690.96611018
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C14:03AFEPAIEM0.98690.96611018
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C03:04SAFEPAIEM0.99970.9949918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C03:03SAFEPAIEM0.99970.9949918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C03:02SAFEPAIEM0.99960.9718918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C03:67SAFEPAIEM0.99950.9912918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C12:02SAFEPAIEM0.99920.9777918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C15:09SAFEPAIEM0.99890.8879918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C03:17SAFEPAIEM0.99830.972918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C15:05SAFEPAIEM0.9980.9397918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C03:05SAFEPAIEM0.9980.9582918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C16:04SAFEPAIEM0.99780.9871918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B35:77SAFEPAIEM0.99780.8945918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C15:02SAFEPAIEM0.99770.91918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B35:23SAFEPAIEM0.99770.8753918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B15:20SAFEPAIEM0.99770.9571918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B35:11SAFEPAIEM0.99750.9074918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B35:20SAFEPAIEM0.99710.9664918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B35:28SAFEPAIEM0.99690.9633918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B15:39SAFEPAIEM0.9960.8851918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B35:17SAFEPAIEM0.99550.7921918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B35:30SAFEPAIEM0.99550.7921918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B15:11SAFEPAIEM0.99510.9145918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B15:08SAFEPAIEM0.9950.9101918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C04:04SAFEPAIEM0.99350.9553918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B35:43SAFEPAIEM0.99330.9109918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C12:03SAFEPAIEM0.99330.9869918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B15:13SAFEPAIEM0.99040.8196918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B35:13SAFEPAIEM0.98850.9197918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C03:06SAFEPAIEM0.9880.9953918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B35:24SAFEPAIEM0.98430.9392918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B48:02SAFEPAIEM0.98380.9584918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C07:04SAFEPAIEM0.97560.9217918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C16:01SAFEPAIEM0.97430.9795918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C06:17SAFEPAIEM0.96190.9953918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C06:02SAFEPAIEM0.96190.9953918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C08:01SAFEPAIEM0.96150.9907918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C02:02SAFEPAIEM0.96110.9759918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C02:10SAFEPAIEM0.96110.9759918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C07:17SAFEPAIEM0.95140.9667918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B35:09SAFEPAIEM0.9440.9724918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C06:06SAFEPAIEM0.93630.9841918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C07:02SAFEPAIEM0.93130.96918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C07:22SAFEPAIEM0.92990.8515918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B07:13SAFEPAIEM0.92540.845918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C16:02SAFEPAIEM0.92240.9907918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B15:30SAFEPAIEM0.91870.7865918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-A25:01SAFEPAIEM0.91220.8403918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C06:08SAFEPAIEM0.9110.9896918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C04:01SAFEPAIEM0.91010.9087918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-A68:02SAFEPAIEM0.90970.5776918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B15:73SAFEPAIEM0.90710.9159918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B78:02SAFEPAIEM0.90050.6987918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C07:01SAFEPAIEM0.89910.8375918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C04:03SAFEPAIEM0.89740.9186918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C05:01SAFEPAIEM0.89030.9588918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C18:01SAFEPAIEM0.88850.9118918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C17:01SAFEPAIEM0.86820.9591918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-A69:01SAFEPAIEM0.85590.7918918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B35:13KPSAFEPAI0.83930.8816716
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B35:09KPSAFEPAI0.83880.9414716
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B67:01SAFEPAIEM0.80280.8452918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C01:03SAFEPAIEM0.77070.9541918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C01:02SAFEPAIEM0.76910.9483918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B39:02SAFEPAIEM0.71680.9676918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-A02:14SAFEPAIEM0.71310.6654918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B18:04SAFEPAIEM0.70910.9694918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-A02:06SAFEPAIEM0.70850.7549918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B67:01KPSAFEPAI0.61770.9022716
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-C08:02SAFEPAIEM0.56060.9799918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B18:07SAFEPAIEM0.52820.9402918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B15:09SAFEPAIEM0.51130.6069918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B18:08SAFEPAIEM0.44110.9506918
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B35:17KPSAFEPAIEM0.99420.7481718
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B35:30KPSAFEPAIEM0.99420.7481718
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B35:13KPSAFEPAIEM0.97780.8889718
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B67:01KPSAFEPAIEM0.95730.8861718
MIEF2-ANKRD30Achr1718166562chr1037440988521HLA-B35:09KPSAFEPAIEM0.9390.9599718

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Potential FusionNeoAntigen Information of MIEF2-ANKRD30A in HLA II

check button Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.
MIEF2-ANKRD30A_18166562_37440988.msa

check button Potential FusionNeoAntigen Information
* We used NetMHCIIpan v4.1 (%rank<0.5).
Fusion geneHchrHbpTgeneTchrTbpHLA IIFusionNeoAntigen peptideNeoantigen start (at BP 13)Neoantigen end (at BP 13)
MIEF2-ANKRD30Achr1718166562chr1037440988521DRB1-0102FEPAIEMQKSVPNKA1126
MIEF2-ANKRD30Achr1718166562chr1037440988521DRB1-0123FEPAIEMQKSVPNKA1126
MIEF2-ANKRD30Achr1718166562chr1037440988521DRB1-0413FEPAIEMQKSVPNKA1126
MIEF2-ANKRD30Achr1718166562chr1037440988521DRB1-0415FEPAIEMQKSVPNKA1126
MIEF2-ANKRD30Achr1718166562chr1037440988521DRB1-0436FEPAIEMQKSVPNKA1126
MIEF2-ANKRD30Achr1718166562chr1037440988521DRB1-0437FEPAIEMQKSVPNKA1126
MIEF2-ANKRD30Achr1718166562chr1037440988521DRB1-0440FEPAIEMQKSVPNKA1126
MIEF2-ANKRD30Achr1718166562chr1037440988521DRB1-0444FEPAIEMQKSVPNKA1126
MIEF2-ANKRD30Achr1718166562chr1037440988521DRB1-0455FEPAIEMQKSVPNKA1126
MIEF2-ANKRD30Achr1718166562chr1037440988521DRB1-0458FEPAIEMQKSVPNKA1126
MIEF2-ANKRD30Achr1718166562chr1037440988521DRB1-0468FEPAIEMQKSVPNKA1126
MIEF2-ANKRD30Achr1718166562chr1037440988521DRB1-0470FEPAIEMQKSVPNKA1126
MIEF2-ANKRD30Achr1718166562chr1037440988521DRB1-0473FEPAIEMQKSVPNKA1126
MIEF2-ANKRD30Achr1718166562chr1037440988521DRB1-0479FEPAIEMQKSVPNKA1126
MIEF2-ANKRD30Achr1718166562chr1037440988521DRB1-0902PSAFEPAIEMQKSVP823
MIEF2-ANKRD30Achr1718166562chr1037440988521DRB1-1001FEPAIEMQKSVPNKA1126
MIEF2-ANKRD30Achr1718166562chr1037440988521DRB1-1002FEPAIEMQKSVPNKA1126
MIEF2-ANKRD30Achr1718166562chr1037440988521DRB1-1002AFEPAIEMQKSVPNK1025
MIEF2-ANKRD30Achr1718166562chr1037440988521DRB1-1003FEPAIEMQKSVPNKA1126
MIEF2-ANKRD30Achr1718166562chr1037440988521DRB1-1452FEPAIEMQKSVPNKA1126
MIEF2-ANKRD30Achr1718166562chr1037440988521DRB1-1457FEPAIEMQKSVPNKA1126
MIEF2-ANKRD30Achr1718166562chr1037440988521DRB3-0204FEPAIEMQKSVPNKA1126
MIEF2-ANKRD30Achr1718166562chr1037440988521DRB4-0101FEPAIEMQKSVPNKA1126
MIEF2-ANKRD30Achr1718166562chr1037440988521DRB4-0103FEPAIEMQKSVPNKA1126
MIEF2-ANKRD30Achr1718166562chr1037440988521DRB4-0104FEPAIEMQKSVPNKA1126
MIEF2-ANKRD30Achr1718166562chr1037440988521DRB4-0106FEPAIEMQKSVPNKA1126
MIEF2-ANKRD30Achr1718166562chr1037440988521DRB4-0107FEPAIEMQKSVPNKA1126
MIEF2-ANKRD30Achr1718166562chr1037440988521DRB4-0108FEPAIEMQKSVPNKA1126
MIEF2-ANKRD30Achr1718166562chr1037440988521DRB5-0101PSAFEPAIEMQKSVP823
MIEF2-ANKRD30Achr1718166562chr1037440988521DRB5-0104PSAFEPAIEMQKSVP823
MIEF2-ANKRD30Achr1718166562chr1037440988521DRB5-0105PSAFEPAIEMQKSVP823
MIEF2-ANKRD30Achr1718166562chr1037440988521DRB5-0112PSAFEPAIEMQKSVP823
MIEF2-ANKRD30Achr1718166562chr1037440988521DRB5-0112KPSAFEPAIEMQKSV722
MIEF2-ANKRD30Achr1718166562chr1037440988521DRB5-0113PSAFEPAIEMQKSVP823
MIEF2-ANKRD30Achr1718166562chr1037440988521DRB5-0114PSAFEPAIEMQKSVP823

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Fusion breakpoint peptide structures of MIEF2-ANKRD30A

check button3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens
* The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA.
File nameBPseqHgeneTgeneHchrHbpTchrTbpAAlen
1861EKPSAFEPAIEMQKMIEF2ANKRD30Achr1718166562chr1037440988521

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of MIEF2-ANKRD30A

check buttonVirtual screening between 25 HLAs (from PDB) and FusionNeoAntigens
* We used Glide to predict the interaction between HLAs and neoantigens.
HLA allelePDB IDFile nameBPseqDocking scoreGlide score
HLA-B14:023BVN1861EKPSAFEPAIEMQK-7.9962-8.1096
HLA-B14:023BVN1861EKPSAFEPAIEMQK-5.70842-6.74372
HLA-B52:013W391861EKPSAFEPAIEMQK-6.83737-6.95077
HLA-B52:013W391861EKPSAFEPAIEMQK-4.4836-5.5189
HLA-A11:014UQ21861EKPSAFEPAIEMQK-10.0067-10.1201
HLA-A11:014UQ21861EKPSAFEPAIEMQK-9.03915-10.0745
HLA-A24:025HGA1861EKPSAFEPAIEMQK-6.56204-6.67544
HLA-A24:025HGA1861EKPSAFEPAIEMQK-5.42271-6.45801
HLA-B44:053DX81861EKPSAFEPAIEMQK-7.85648-8.89178
HLA-B44:053DX81861EKPSAFEPAIEMQK-5.3978-5.5112
HLA-A02:016TDR1861EKPSAFEPAIEMQK-3.37154-4.40684

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Vaccine Design for the FusionNeoAntigens of MIEF2-ANKRD30A

check button mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is.
Fusion geneHchrHbpTchrTbpStart in +/-13AAEnd in +/-13AAFusionNeoAntigen peptide sequenceFusionNeoAntigen RNA sequence
MIEF2-ANKRD30Achr1718166562chr10374409881018AFEPAIEMCTGCCCCAGATCCGATGTTCCCAC
MIEF2-ANKRD30Achr1718166562chr1037440988716KPSAFEPAICCTCGTCGTCTGCCCCAGATCCGATGT
MIEF2-ANKRD30Achr1718166562chr1037440988718KPSAFEPAIEMCCTCGTCGTCTGCCCCAGATCCGATGTTCCCAC
MIEF2-ANKRD30Achr1718166562chr1037440988918SAFEPAIEMCGTCTGCCCCAGATCCGATGTTCCCAC

check button mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs.
Fusion geneHchrHbpTchrTbpStart in +/-13AAEnd in +/-13AAFusionNeoAntigen peptideFusionNEoAntigen RNA sequence
MIEF2-ANKRD30Achr1718166562chr10374409881025AFEPAIEMQKSVPNKCTGCCCCAGATCCGATGTTCCCACCAGAATCCAAACAAAAGGACT
MIEF2-ANKRD30Achr1718166562chr10374409881126FEPAIEMQKSVPNKACCCCAGATCCGATGTTCCCACCAGAATCCAAACAAAAGGACTATG
MIEF2-ANKRD30Achr1718166562chr1037440988722KPSAFEPAIEMQKSVCCTCGTCGTCTGCCCCAGATCCGATGTTCCCACCAGAATCCAAAC
MIEF2-ANKRD30Achr1718166562chr1037440988823PSAFEPAIEMQKSVPCGTCGTCTGCCCCAGATCCGATGTTCCCACCAGAATCCAAACAAA

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Information of the samples that have these potential fusion neoantigens of MIEF2-ANKRD30A

check button These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens.
Cancer typeFusion geneHchrHbpHenstTchrTbpTenstSample
BRCAMIEF2-ANKRD30Achr1718166562ENST00000323019chr1037440988ENST00000361713TCGA-AR-A2LR-01A

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Potential target of CAR-T therapy development for MIEF2-ANKRD30A

check button Predicted 3D structure. We used RoseTTAFold.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features


* Minus value of BPloci means that the break point is located before the CDS.
- In-frame and retained 'Transmembrane'.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneMIEF2chr17:18166562chr10:37440988ENST00000323019+3423_43103455.0TransmembraneHelical
HgeneMIEF2chr17:18166562chr10:37440988ENST00000395704+3423_43103206.0TransmembraneHelical
HgeneMIEF2chr17:18166562chr10:37440988ENST00000395706+3423_43114466.0TransmembraneHelical

check button Subcellular localization prediction of the transmembrane domain retained fusion proteins
* We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image.
HgeneHchrHbpHenstTgeneTchrTbpTenstDeepLoc result

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Related Drugs to MIEF2-ANKRD30A

check button Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDrugSourcePMID

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Related Diseases to MIEF2-ANKRD30A

check button Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDiseaseSourcePMID

check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource