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Fusion Protein:MPHOSPH8-ATP8A2 |
Fusion Gene and Fusion Protein Summary |
 Fusion gene summary |
| Fusion partner gene information | Fusion gene name: MPHOSPH8-ATP8A2 | FusionPDB ID: 54691 | FusionGDB2.0 ID: 54691 | Hgene | Tgene | Gene symbol | MPHOSPH8 | ATP8A2 | Gene ID | 54737 | 51761 |
| Gene name | M-phase phosphoprotein 8 | ATPase phospholipid transporting 8A2 | |
| Synonyms | HSMPP8|TWA3|mpp8 | ATP|ATPIB|CAMRQ4|IB|ML-1 | |
| Cytomap | 13q12.11 | 13q12.13 | |
| Type of gene | protein-coding | protein-coding | |
| Description | M-phase phosphoprotein 8M-phase phosphoprotein, mppM-phase phosphoprotein, mpp8two hybrid-associated protein 3 with RanBPM | phospholipid-transporting ATPase IBATPase, aminophospholipid transporter, class I, type 8A, member 2ATPase, aminophospholipid transporter-like, class I, type 8A, member 2P4-ATPase flippase complex alpha subunit ATP8A2probable phospholipid-transporting | |
| Modification date | 20200313 | 20200329 | |
| UniProtAcc | Q99549 Main function of 5'-partner protein: FUNCTION: Heterochromatin component that specifically recognizes and binds methylated 'Lys-9' of histone H3 (H3K9me) and promotes recruitment of proteins that mediate epigenetic repression (PubMed:20871592, PubMed:26022416). Mediates recruitment of the HUSH complex to H3K9me3 sites: the HUSH complex is recruited to genomic loci rich in H3K9me3 and is required to maintain transcriptional silencing by promoting recruitment of SETDB1, a histone methyltransferase that mediates further deposition of H3K9me3, as well as MORC2 (PubMed:26022416, PubMed:28581500). Binds H3K9me and promotes DNA methylation by recruiting DNMT3A to target CpG sites; these can be situated within the coding region of the gene (PubMed:20871592). Mediates down-regulation of CDH1 expression (PubMed:20871592). Also represses L1 retrotransposons in collaboration with MORC2 and, probably, SETDB1, the silencing is dependent of repressive epigenetic modifications, such as H3K9me3 mark. Silencing events often occur within introns of transcriptionally active genes, and lead to the down-regulation of host gene expression (PubMed:29211708). The HUSH complex is also involved in the silencing of unintegrated retroviral DNA by being recruited by ZNF638: some part of the retroviral DNA formed immediately after infection remains unintegrated in the host genome and is transcriptionally repressed (PubMed:30487602). {ECO:0000269|PubMed:20871592, ECO:0000269|PubMed:26022416, ECO:0000269|PubMed:28581500, ECO:0000269|PubMed:29211708, ECO:0000269|PubMed:30487602}. | Q9NTI2 Main function of 5'-partner protein: FUNCTION: Catalytic component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids. Phospholipid translocation seems also to be implicated in vesicle formation and in uptake of lipid signaling molecules. Reconstituted to liposomes, the ATP8A2:TMEM30A flippase complex predominantly transports phosphatidylserine (PS) and to a lesser extent phosphatidylethanolamine (PE). Phospholipid translocation is not associated with a countertransport of an inorganic ion or other charged substrate from the cytoplasmic side toward the exoplasm in connection with the phosphorylation from ATP. ATP8A2:TMEM30A may be involved in regulation of neurite outgrowth. Proposed to function in the generation and maintenance of phospholipid asymmetry in photoreceptor disk membranes and neuronal axon membranes. May be involved in vesicle trafficking in neuronal cells. Required for normal visual and auditory function; involved in photoreceptor and inner ear spiral ganglion cell survival. {ECO:0000250|UniProtKB:C7EXK4}. | |
| Ensembl transtripts involved in fusion gene | ENST ids | ENST00000361479, ENST00000414242, ENST00000496525, | ENST00000491840, ENST00000255283, ENST00000381655, |
| Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0) | * DoF score | 8 X 11 X 5=440 | 13 X 13 X 7=1183 |
| # samples | 12 | 15 | |
| ** MAII score | log2(12/440*10)=-1.87446911791614 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | log2(15/1183*10)=-2.97941566784391 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | |
| Fusion gene context | PubMed: MPHOSPH8 [Title/Abstract] AND ATP8A2 [Title/Abstract] AND fusion [Title/Abstract] | ||
| Fusion neoantigen context | PubMed: MPHOSPH8 [Title/Abstract] AND ATP8A2 [Title/Abstract] AND neoantigen [Title/Abstract] | ||
| Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0) | MPHOSPH8(20208101)-ATP8A2(26535713), # samples:1 | ||
| Anticipated loss of major functional domain due to fusion event. | MPHOSPH8-ATP8A2 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. MPHOSPH8-ATP8A2 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. | ||
| * DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
| Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
| Partner | Gene | GO ID | GO term | PubMed ID |
| Hgene | MPHOSPH8 | GO:0045814 | negative regulation of gene expression, epigenetic | 26022416|28581500|29211708 |
| Hgene | MPHOSPH8 | GO:0090309 | positive regulation of methylation-dependent chromatin silencing | 28581500|29211708 |
| Four levels of functional features of fusion genes Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr13:20208101/chr13:26535713) - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. - How to search 1. Put your fusion gene symbol. 2. Press the tab key until there will be shown the breakpoint information filled. 4. Go down and press 'Search' tab twice. 4. Go down to have the hyperlink of the search result. 5. Click the hyperlink. 6. See the FGviewer result for your fusion gene. |
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| Retention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here. |
| Fusion gene breakpoints across MPHOSPH8 (5'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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| Fusion gene breakpoints across ATP8A2 (3'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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Fusion Amino Acid Sequences |
| Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
| ENST00000414242 | MPHOSPH8 | chr13 | 20208101 | + | ENST00000381655 | ATP8A2 | chr13 | 26535713 | + | 6564 | 314 | 101 | 697 | 198 |
| ENST00000414242 | MPHOSPH8 | chr13 | 20208101 | + | ENST00000255283 | ATP8A2 | chr13 | 26535713 | + | 954 | 314 | 101 | 697 | 198 |
| ENST00000361479 | MPHOSPH8 | chr13 | 20208101 | + | ENST00000381655 | ATP8A2 | chr13 | 26535713 | + | 6531 | 281 | 68 | 664 | 198 |
| ENST00000361479 | MPHOSPH8 | chr13 | 20208101 | + | ENST00000255283 | ATP8A2 | chr13 | 26535713 | + | 921 | 281 | 68 | 664 | 198 |
| DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
| ENST00000414242 | ENST00000381655 | MPHOSPH8 | chr13 | 20208101 | + | ATP8A2 | chr13 | 26535713 | + | 0.001140504 | 0.9988595 |
| ENST00000414242 | ENST00000255283 | MPHOSPH8 | chr13 | 20208101 | + | ATP8A2 | chr13 | 26535713 | + | 0.00187954 | 0.9981205 |
| ENST00000361479 | ENST00000381655 | MPHOSPH8 | chr13 | 20208101 | + | ATP8A2 | chr13 | 26535713 | + | 0.001146553 | 0.99885345 |
| ENST00000361479 | ENST00000255283 | MPHOSPH8 | chr13 | 20208101 | + | ATP8A2 | chr13 | 26535713 | + | 0.002173028 | 0.997827 |
| Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones. |
Get the fusion protein sequences from here. |
| Fusion protein sequence information is available in the fasta format. >FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP |
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Fusion Protein Breakpoint Sequences for MPHOSPH8-ATP8A2 |
| +/-13 AA sequence from the breakpoints of the fusion protein sequences. |
| Hgene | Hchr | Hbp | Tgene | Tchr | Tbp | Length(fusion protein) | BP in fusion protein | Peptide |
| MPHOSPH8 | chr13 | 20208101 | ATP8A2 | chr13 | 26535713 | 281 | 71 | FEVEKILDMKTEGATMVLSSAHFWLG |
| MPHOSPH8 | chr13 | 20208101 | ATP8A2 | chr13 | 26535713 | 314 | 71 | FEVEKILDMKTEGATMVLSSAHFWLG |
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Potential FusionNeoAntigen Information of MPHOSPH8-ATP8A2 in HLA I |
| Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific. |
| MPHOSPH8-ATP8A2_20208101_26535713.msa |
| Potential FusionNeoAntigen Information * We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5) |
| Fusion gene | Hchr | Hbp | Tgene | Tchr | Tbp | HLA I | FusionNeoAntigen peptide | Binding score | Immunogenic score | Neoantigen start (at BP 13) | Neoantigen end (at BP 13) |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | HLA-B15:02 | DMKTEGATM | 0.7254 | 0.9747 | 7 | 16 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | HLA-B08:01 | DMKTEGATM | 0.6895 | 0.8143 | 7 | 16 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | HLA-C05:09 | KTEGATMVL | 0.9999 | 0.9172 | 9 | 18 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | HLA-C08:15 | KTEGATMVL | 0.9998 | 0.9612 | 9 | 18 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | HLA-C03:07 | KTEGATMVL | 0.9957 | 0.9187 | 9 | 18 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | HLA-C15:06 | KTEGATMVL | 0.9935 | 0.8539 | 9 | 18 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | HLA-C03:19 | KTEGATMVL | 0.9898 | 0.9734 | 9 | 18 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | HLA-C03:08 | KTEGATMVL | 0.9841 | 0.7972 | 9 | 18 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | HLA-C04:06 | KTEGATMVL | 0.9724 | 0.9176 | 9 | 18 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | HLA-C08:13 | KTEGATMVL | 0.9319 | 0.9487 | 9 | 18 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | HLA-C08:04 | KTEGATMVL | 0.9319 | 0.9487 | 9 | 18 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | HLA-C08:03 | KTEGATMVL | 0.7353 | 0.9741 | 9 | 18 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | HLA-B15:21 | DMKTEGATM | 0.7322 | 0.9576 | 7 | 16 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | HLA-C05:01 | KTEGATMVL | 0.9999 | 0.9172 | 9 | 18 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | HLA-C04:03 | KTEGATMVL | 0.9999 | 0.8809 | 9 | 18 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | HLA-C08:02 | KTEGATMVL | 0.9998 | 0.9612 | 9 | 18 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | HLA-C15:05 | KTEGATMVL | 0.9934 | 0.8582 | 9 | 18 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | HLA-C15:02 | KTEGATMVL | 0.9922 | 0.8287 | 9 | 18 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | HLA-C03:06 | KTEGATMVL | 0.8608 | 0.978 | 9 | 18 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | HLA-C08:01 | KTEGATMVL | 0.7353 | 0.9741 | 9 | 18 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | HLA-B08:18 | DMKTEGATM | 0.6895 | 0.8143 | 7 | 16 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | HLA-B15:08 | DMKTEGATM | 0.687 | 0.9101 | 7 | 16 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | HLA-B08:12 | DMKTEGATM | 0.6399 | 0.8397 | 7 | 16 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | HLA-C17:01 | KTEGATMVL | 0.3254 | 0.8816 | 9 | 18 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | HLA-B07:13 | KTEGATMVL | 0.0331 | 0.6466 | 9 | 18 |
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Potential FusionNeoAntigen Information of MPHOSPH8-ATP8A2 in HLA II |
| Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific. |
| MPHOSPH8-ATP8A2_20208101_26535713.msa |
| Potential FusionNeoAntigen Information * We used NetMHCIIpan v4.1 (%rank<0.5). |
| Fusion gene | Hchr | Hbp | Tgene | Tchr | Tbp | HLA II | FusionNeoAntigen peptide | Neoantigen start (at BP 13) | Neoantigen end (at BP 13) |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0403 | VEKILDMKTEGATMV | 2 | 17 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0403 | EVEKILDMKTEGATM | 1 | 16 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0413 | VEKILDMKTEGATMV | 2 | 17 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0413 | EVEKILDMKTEGATM | 1 | 16 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0413 | FEVEKILDMKTEGAT | 0 | 15 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0415 | VEKILDMKTEGATMV | 2 | 17 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0415 | EVEKILDMKTEGATM | 1 | 16 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0415 | FEVEKILDMKTEGAT | 0 | 15 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0419 | VEKILDMKTEGATMV | 2 | 17 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0419 | EVEKILDMKTEGATM | 1 | 16 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0427 | VEKILDMKTEGATMV | 2 | 17 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0427 | EVEKILDMKTEGATM | 1 | 16 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0427 | FEVEKILDMKTEGAT | 0 | 15 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0436 | VEKILDMKTEGATMV | 2 | 17 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0436 | EVEKILDMKTEGATM | 1 | 16 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0436 | FEVEKILDMKTEGAT | 0 | 15 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0437 | VEKILDMKTEGATMV | 2 | 17 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0437 | EVEKILDMKTEGATM | 1 | 16 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0439 | VEKILDMKTEGATMV | 2 | 17 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0439 | EVEKILDMKTEGATM | 1 | 16 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0440 | VEKILDMKTEGATMV | 2 | 17 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0440 | EVEKILDMKTEGATM | 1 | 16 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0440 | FEVEKILDMKTEGAT | 0 | 15 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0441 | VEKILDMKTEGATMV | 2 | 17 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0441 | EVEKILDMKTEGATM | 1 | 16 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0442 | VEKILDMKTEGATMV | 2 | 17 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0442 | EVEKILDMKTEGATM | 1 | 16 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0442 | FEVEKILDMKTEGAT | 0 | 15 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0444 | VEKILDMKTEGATMV | 2 | 17 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0444 | EVEKILDMKTEGATM | 1 | 16 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0444 | FEVEKILDMKTEGAT | 0 | 15 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0446 | VEKILDMKTEGATMV | 2 | 17 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0446 | EVEKILDMKTEGATM | 1 | 16 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0449 | VEKILDMKTEGATMV | 2 | 17 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0449 | EVEKILDMKTEGATM | 1 | 16 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0450 | VEKILDMKTEGATMV | 2 | 17 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0450 | EVEKILDMKTEGATM | 1 | 16 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0450 | FEVEKILDMKTEGAT | 0 | 15 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0451 | VEKILDMKTEGATMV | 2 | 17 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0451 | EVEKILDMKTEGATM | 1 | 16 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0451 | FEVEKILDMKTEGAT | 0 | 15 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0452 | VEKILDMKTEGATMV | 2 | 17 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0452 | EVEKILDMKTEGATM | 1 | 16 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0453 | VEKILDMKTEGATMV | 2 | 17 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0453 | EVEKILDMKTEGATM | 1 | 16 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0453 | FEVEKILDMKTEGAT | 0 | 15 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0455 | VEKILDMKTEGATMV | 2 | 17 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0455 | EVEKILDMKTEGATM | 1 | 16 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0455 | FEVEKILDMKTEGAT | 0 | 15 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0456 | VEKILDMKTEGATMV | 2 | 17 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0456 | EVEKILDMKTEGATM | 1 | 16 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0456 | FEVEKILDMKTEGAT | 0 | 15 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0458 | VEKILDMKTEGATMV | 2 | 17 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0458 | EVEKILDMKTEGATM | 1 | 16 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0458 | FEVEKILDMKTEGAT | 0 | 15 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0459 | VEKILDMKTEGATMV | 2 | 17 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0459 | EVEKILDMKTEGATM | 1 | 16 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0460 | VEKILDMKTEGATMV | 2 | 17 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0460 | EVEKILDMKTEGATM | 1 | 16 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0461 | VEKILDMKTEGATMV | 2 | 17 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0461 | EVEKILDMKTEGATM | 1 | 16 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0465 | VEKILDMKTEGATMV | 2 | 17 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0468 | VEKILDMKTEGATMV | 2 | 17 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0468 | EVEKILDMKTEGATM | 1 | 16 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0468 | FEVEKILDMKTEGAT | 0 | 15 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0470 | VEKILDMKTEGATMV | 2 | 17 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0470 | EVEKILDMKTEGATM | 1 | 16 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0470 | FEVEKILDMKTEGAT | 0 | 15 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0471 | VEKILDMKTEGATMV | 2 | 17 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0471 | EVEKILDMKTEGATM | 1 | 16 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0473 | VEKILDMKTEGATMV | 2 | 17 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0473 | EVEKILDMKTEGATM | 1 | 16 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0473 | FEVEKILDMKTEGAT | 0 | 15 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0475 | VEKILDMKTEGATMV | 2 | 17 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0478 | VEKILDMKTEGATMV | 2 | 17 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0478 | EVEKILDMKTEGATM | 1 | 16 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0479 | VEKILDMKTEGATMV | 2 | 17 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0479 | EVEKILDMKTEGATM | 1 | 16 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0479 | FEVEKILDMKTEGAT | 0 | 15 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0485 | VEKILDMKTEGATMV | 2 | 17 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0485 | EVEKILDMKTEGATM | 1 | 16 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0488 | VEKILDMKTEGATMV | 2 | 17 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-0488 | EVEKILDMKTEGATM | 1 | 16 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-1002 | VEKILDMKTEGATMV | 2 | 17 |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 | DRB1-1002 | EVEKILDMKTEGATM | 1 | 16 |
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Fusion breakpoint peptide structures of MPHOSPH8-ATP8A2 |
| 3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens * The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA. |
| File name | BPseq | Hgene | Tgene | Hchr | Hbp | Tchr | Tbp | AAlen |
| 4852 | LDMKTEGATMVLSS | MPHOSPH8 | ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 281 |
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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of MPHOSPH8-ATP8A2 |
| Virtual screening between 25 HLAs (from PDB) and FusionNeoAntigens * We used Glide to predict the interaction between HLAs and neoantigens. |
| HLA allele | PDB ID | File name | BPseq | Docking score | Glide score |
| HLA-B14:02 | 3BVN | 4852 | LDMKTEGATMVLSS | -7.9962 | -8.1096 |
| HLA-B14:02 | 3BVN | 4852 | LDMKTEGATMVLSS | -5.70842 | -6.74372 |
| HLA-B52:01 | 3W39 | 4852 | LDMKTEGATMVLSS | -6.83737 | -6.95077 |
| HLA-B52:01 | 3W39 | 4852 | LDMKTEGATMVLSS | -4.4836 | -5.5189 |
| HLA-A11:01 | 4UQ2 | 4852 | LDMKTEGATMVLSS | -10.0067 | -10.1201 |
| HLA-A11:01 | 4UQ2 | 4852 | LDMKTEGATMVLSS | -9.03915 | -10.0745 |
| HLA-A24:02 | 5HGA | 4852 | LDMKTEGATMVLSS | -6.56204 | -6.67544 |
| HLA-A24:02 | 5HGA | 4852 | LDMKTEGATMVLSS | -5.42271 | -6.45801 |
| HLA-B44:05 | 3DX8 | 4852 | LDMKTEGATMVLSS | -7.85648 | -8.89178 |
| HLA-B44:05 | 3DX8 | 4852 | LDMKTEGATMVLSS | -5.3978 | -5.5112 |
| HLA-A02:01 | 6TDR | 4852 | LDMKTEGATMVLSS | -3.37154 | -4.40684 |
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Vaccine Design for the FusionNeoAntigens of MPHOSPH8-ATP8A2 |
| mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is. |
| Fusion gene | Hchr | Hbp | Tchr | Tbp | Start in +/-13AA | End in +/-13AA | FusionNeoAntigen peptide sequence | FusionNeoAntigen RNA sequence |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 7 | 16 | DMKTEGATM | GACATGAAGACCGAGGGGGCAACTATG |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 9 | 18 | KTEGATMVL | AAGACCGAGGGGGCAACTATGGTCCTG |
| mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs. |
| Fusion gene | Hchr | Hbp | Tchr | Tbp | Start in +/-13AA | End in +/-13AA | FusionNeoAntigen peptide | FusionNEoAntigen RNA sequence |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 0 | 15 | FEVEKILDMKTEGAT | TTCGAGGTGGAGAAGATCCTGGACATGAAGACCGAGGGGGCAACT |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 1 | 16 | EVEKILDMKTEGATM | GAGGTGGAGAAGATCCTGGACATGAAGACCGAGGGGGCAACTATG |
| MPHOSPH8-ATP8A2 | chr13 | 20208101 | chr13 | 26535713 | 2 | 17 | VEKILDMKTEGATMV | GTGGAGAAGATCCTGGACATGAAGACCGAGGGGGCAACTATGGTC |
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Information of the samples that have these potential fusion neoantigens of MPHOSPH8-ATP8A2 |
| These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens. |
| Cancer type | Fusion gene | Hchr | Hbp | Henst | Tchr | Tbp | Tenst | Sample |
| COAD | MPHOSPH8-ATP8A2 | chr13 | 20208101 | ENST00000361479 | chr13 | 26535713 | ENST00000255283 | TCGA-AY-A54L-01A |
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Potential target of CAR-T therapy development for MPHOSPH8-ATP8A2 |
| Predicted 3D structure. We used RoseTTAFold. |
| Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features * Minus value of BPloci means that the break point is located before the CDS. |
| - In-frame and retained 'Transmembrane'. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| Tgene | ATP8A2 | chr13:20208101 | chr13:26535713 | ENST00000381655 | 32 | 37 | 1064_1084 | 0 | 1189.0 | Transmembrane | Helical |
| Subcellular localization prediction of the transmembrane domain retained fusion proteins * We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image. |
| Hgene | Hchr | Hbp | Henst | Tgene | Tchr | Tbp | Tenst | DeepLoc result |
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Related Drugs to MPHOSPH8-ATP8A2 |
| Drugs used for this fusion-positive patient. (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
| Hgene | Tgene | Drug | Source | PMID |
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Related Diseases to MPHOSPH8-ATP8A2 |
| Diseases that have this fusion gene. (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
| Hgene | Tgene | Disease | Source | PMID |
| Diseases associated with fusion partners. (DisGeNet 4.0) |
| Partner | Gene | Disease ID | Disease name | # pubmeds | Source |
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