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Center for Computational Systems Medicine
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Fusion Gene and Fusion Protein Summary

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Fusion Amino Acid Sequences (multiple BPs and multiple gene isoforms)

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Fusion Protein Breakpoint Sequences - (for the Screening of the FusionNeoAntigens)

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Potential FusionNeoAntigens in HLA I - (netMHCpan v4.1 + deepHLApan v1.1)

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Potential FusionNeoAntigens in HLA II - (netMHCIIpan v4.1)

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Fusion Breakpoint 14 AA Peptide Structure - (RoseTTAFold)

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D - (Glide)

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Vaccine Design for the FusionNeoAntigens (RNA/protein sequences)

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Potential target of CAR-T therapy development

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Information on the samples that have these potential fusion neoantigens

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Fusion Protein Targeting Drugs - (Manual Curation)

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Fusion Protein Related diseases - (Manual Curation)

Fusion Protein:MTUS1-FGL1

Fusion Gene and Fusion Protein Summary

check button Fusion gene summary
Fusion partner gene informationFusion gene name: MTUS1-FGL1
FusionPDB ID: 55936
FusionGDB2.0 ID: 55936
HgeneTgene
Gene symbol

MTUS1

FGL1

Gene ID

57509

2267

Gene namemicrotubule associated scaffold protein 1fibrinogen like 1
SynonymsATBP|ATIP|ATIP3|ICIS|MP44|MTSG1HFREP1|HP-041|HPS|LFIRE-1|LFIRE1
Cytomap

8p22

8p22

Type of geneprotein-codingprotein-coding
Descriptionmicrotubule-associated tumor suppressor 1AT2 receptor-binding proteinAT2 receptor-interacting proteinAT2R binding proteinangiotensin-II type 2 receptor-interacting proteinerythroid differentiation-relatedmicrotubule associated tumor suppressor 1mitfibrinogen-like protein 1hepassocinhepatocellular carcinoma-related sequencehepatocyte-derived fibrinogen-related protein 1liver fibrinogen-related protein 1
Modification date2020031320200313
UniProtAcc

Q9ULD2

Main function of 5'-partner protein: FUNCTION: Cooperates with AGTR2 to inhibit ERK2 activation and cell proliferation. May be required for AGTR2 cell surface expression. Together with PTPN6, induces UBE2V2 expression upon angiotensin-II stimulation. Isoform 1 inhibits breast cancer cell proliferation, delays the progression of mitosis by prolonging metaphase and reduces tumor growth. {ECO:0000269|PubMed:12692079, ECO:0000269|PubMed:19794912}.		.
Ensembl transtripts involved in fusion geneENST idsENST00000262102, ENST00000381861, 
ENST00000381869, ENST00000519263, 
ENST00000544260, ENST00000297488, 
ENST00000381862, ENST00000400046, 
ENST00000518713, 		ENST00000381840, 
ENST00000381841, ENST00000398054, 
ENST00000398056, ENST00000427924, 
ENST00000518650, ENST00000522444, 
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)* DoF score12 X 13 X 5=7804 X 5 X 5=100
# samples 156
** MAII scorelog2(15/780*10)=-2.37851162325373
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(6/100*10)=-0.736965594166206
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Fusion gene context

PubMed: MTUS1 [Title/Abstract] AND FGL1 [Title/Abstract] AND fusion [Title/Abstract]

Fusion neoantigen context

PubMed: MTUS1 [Title/Abstract] AND FGL1 [Title/Abstract] AND neoantigen [Title/Abstract]

Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)MTUS1(17570723)-FGL1(17743080), # samples:4
Anticipated loss of major functional domain due to fusion event.MTUS1-FGL1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
MTUS1-FGL1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
MTUS1-FGL1 seems lost the major protein functional domain in Hgene partner, which is a tumor suppressor due to the frame-shifted ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
TgeneFGL1

GO:0072574

hepatocyte proliferation

19880967



check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr8:17570723/chr8:17743080)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonRetention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here.

check buttonFusion gene breakpoints across MTUS1 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check buttonFusion gene breakpoints across FGL1 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure


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Fusion Amino Acid Sequences


check buttonFusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000544260MTUS1chr817570723-ENST00000398056FGL1chr817726513-978329235765176
ENST00000544260MTUS1chr817570723-ENST00000522444FGL1chr817726513-969329235765176
ENST00000544260MTUS1chr817570723-ENST00000398054FGL1chr817726513-965329235765176
ENST00000544260MTUS1chr817570723-ENST00000381840FGL1chr817726513-965329235765176
ENST00000544260MTUS1chr817570723-ENST00000381841FGL1chr817726513-965329235765176
ENST00000544260MTUS1chr817570723-ENST00000427924FGL1chr817726513-965329235765176
ENST00000544260MTUS1chr817570723-ENST00000518650FGL1chr817726513-774329235765176
ENST00000381861MTUS1chr817570723-ENST00000398056FGL1chr817726513-13336843201120266
ENST00000381861MTUS1chr817570723-ENST00000522444FGL1chr817726513-13246843201120266
ENST00000381861MTUS1chr817570723-ENST00000398054FGL1chr817726513-13206843201120266
ENST00000381861MTUS1chr817570723-ENST00000381840FGL1chr817726513-13206843201120266
ENST00000381861MTUS1chr817570723-ENST00000381841FGL1chr817726513-13206843201120266
ENST00000381861MTUS1chr817570723-ENST00000427924FGL1chr817726513-13206843201120266
ENST00000381861MTUS1chr817570723-ENST00000518650FGL1chr817726513-11296843201120266
ENST00000519263MTUS1chr817570723-ENST00000398056FGL1chr817726513-328426351743071965
ENST00000519263MTUS1chr817570723-ENST00000522444FGL1chr817726513-327526351743071965
ENST00000519263MTUS1chr817570723-ENST00000398054FGL1chr817726513-327126351743071965
ENST00000519263MTUS1chr817570723-ENST00000381840FGL1chr817726513-327126351743071965
ENST00000519263MTUS1chr817570723-ENST00000381841FGL1chr817726513-327126351743071965
ENST00000519263MTUS1chr817570723-ENST00000427924FGL1chr817726513-327126351743071965
ENST00000519263MTUS1chr817570723-ENST00000518650FGL1chr817726513-308026351743071965
ENST00000544260MTUS1chr817570722-ENST00000398056FGL1chr817726513-978329235765176
ENST00000544260MTUS1chr817570722-ENST00000522444FGL1chr817726513-969329235765176
ENST00000544260MTUS1chr817570722-ENST00000398054FGL1chr817726513-965329235765176
ENST00000544260MTUS1chr817570722-ENST00000381840FGL1chr817726513-965329235765176
ENST00000544260MTUS1chr817570722-ENST00000381841FGL1chr817726513-965329235765176
ENST00000544260MTUS1chr817570722-ENST00000427924FGL1chr817726513-965329235765176
ENST00000544260MTUS1chr817570722-ENST00000518650FGL1chr817726513-774329235765176
ENST00000381861MTUS1chr817570722-ENST00000398056FGL1chr817726513-13336843201120266
ENST00000381861MTUS1chr817570722-ENST00000522444FGL1chr817726513-13246843201120266
ENST00000381861MTUS1chr817570722-ENST00000398054FGL1chr817726513-13206843201120266
ENST00000381861MTUS1chr817570722-ENST00000381840FGL1chr817726513-13206843201120266
ENST00000381861MTUS1chr817570722-ENST00000381841FGL1chr817726513-13206843201120266
ENST00000381861MTUS1chr817570722-ENST00000427924FGL1chr817726513-13206843201120266
ENST00000381861MTUS1chr817570722-ENST00000518650FGL1chr817726513-11296843201120266
ENST00000519263MTUS1chr817570722-ENST00000398056FGL1chr817726513-328426351743071965
ENST00000519263MTUS1chr817570722-ENST00000522444FGL1chr817726513-327526351743071965
ENST00000519263MTUS1chr817570722-ENST00000398054FGL1chr817726513-327126351743071965
ENST00000519263MTUS1chr817570722-ENST00000381840FGL1chr817726513-327126351743071965
ENST00000519263MTUS1chr817570722-ENST00000381841FGL1chr817726513-327126351743071965
ENST00000519263MTUS1chr817570722-ENST00000427924FGL1chr817726513-327126351743071965
ENST00000519263MTUS1chr817570722-ENST00000518650FGL1chr817726513-308026351743071965
ENST00000544260MTUS1chr817570722-ENST00000398056FGL1chr817726512-978329235765176
ENST00000544260MTUS1chr817570722-ENST00000522444FGL1chr817726512-969329235765176
ENST00000544260MTUS1chr817570722-ENST00000398054FGL1chr817726512-965329235765176
ENST00000544260MTUS1chr817570722-ENST00000381840FGL1chr817726512-965329235765176
ENST00000544260MTUS1chr817570722-ENST00000381841FGL1chr817726512-965329235765176
ENST00000544260MTUS1chr817570722-ENST00000427924FGL1chr817726512-965329235765176
ENST00000544260MTUS1chr817570722-ENST00000518650FGL1chr817726512-774329235765176
ENST00000381861MTUS1chr817570722-ENST00000398056FGL1chr817726512-13336843201120266
ENST00000381861MTUS1chr817570722-ENST00000522444FGL1chr817726512-13246843201120266
ENST00000381861MTUS1chr817570722-ENST00000398054FGL1chr817726512-13206843201120266
ENST00000381861MTUS1chr817570722-ENST00000381840FGL1chr817726512-13206843201120266
ENST00000381861MTUS1chr817570722-ENST00000381841FGL1chr817726512-13206843201120266
ENST00000381861MTUS1chr817570722-ENST00000427924FGL1chr817726512-13206843201120266
ENST00000381861MTUS1chr817570722-ENST00000518650FGL1chr817726512-11296843201120266
ENST00000519263MTUS1chr817570722-ENST00000398056FGL1chr817726512-328426351743071965
ENST00000519263MTUS1chr817570722-ENST00000522444FGL1chr817726512-327526351743071965
ENST00000519263MTUS1chr817570722-ENST00000398054FGL1chr817726512-327126351743071965
ENST00000519263MTUS1chr817570722-ENST00000381840FGL1chr817726512-327126351743071965
ENST00000519263MTUS1chr817570722-ENST00000381841FGL1chr817726512-327126351743071965
ENST00000519263MTUS1chr817570722-ENST00000427924FGL1chr817726512-327126351743071965
ENST00000519263MTUS1chr817570722-ENST00000518650FGL1chr817726512-308026351743071965

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000544260ENST00000398056MTUS1chr817570723-FGL1chr817726513-0.0047309240.995269
ENST00000544260ENST00000522444MTUS1chr817570723-FGL1chr817726513-0.0047909750.99520904
ENST00000544260ENST00000398054MTUS1chr817570723-FGL1chr817726513-0.0048366390.9951633
ENST00000544260ENST00000381840MTUS1chr817570723-FGL1chr817726513-0.0048366390.9951633
ENST00000544260ENST00000381841MTUS1chr817570723-FGL1chr817726513-0.0048366390.9951633
ENST00000544260ENST00000427924MTUS1chr817570723-FGL1chr817726513-0.0048366390.9951633
ENST00000544260ENST00000518650MTUS1chr817570723-FGL1chr817726513-0.0050588650.9949411
ENST00000381861ENST00000398056MTUS1chr817570723-FGL1chr817726513-0.0010308770.9989691
ENST00000381861ENST00000522444MTUS1chr817570723-FGL1chr817726513-0.0010535190.9989465
ENST00000381861ENST00000398054MTUS1chr817570723-FGL1chr817726513-0.0010935760.9989064
ENST00000381861ENST00000381840MTUS1chr817570723-FGL1chr817726513-0.0010935760.9989064
ENST00000381861ENST00000381841MTUS1chr817570723-FGL1chr817726513-0.0010935760.9989064
ENST00000381861ENST00000427924MTUS1chr817570723-FGL1chr817726513-0.0010935760.9989064
ENST00000381861ENST00000518650MTUS1chr817570723-FGL1chr817726513-0.0010203310.9989796
ENST00000519263ENST00000398056MTUS1chr817570723-FGL1chr817726513-0.0002705450.99972945
ENST00000519263ENST00000522444MTUS1chr817570723-FGL1chr817726513-0.0002727810.9997272
ENST00000519263ENST00000398054MTUS1chr817570723-FGL1chr817726513-0.0002818150.9997181
ENST00000519263ENST00000381840MTUS1chr817570723-FGL1chr817726513-0.0002818150.9997181
ENST00000519263ENST00000381841MTUS1chr817570723-FGL1chr817726513-0.0002818150.9997181
ENST00000519263ENST00000427924MTUS1chr817570723-FGL1chr817726513-0.0002818150.9997181
ENST00000519263ENST00000518650MTUS1chr817570723-FGL1chr817726513-0.0003296040.9996704
ENST00000544260ENST00000398056MTUS1chr817570722-FGL1chr817726513-0.0047309240.995269
ENST00000544260ENST00000522444MTUS1chr817570722-FGL1chr817726513-0.0047909750.99520904
ENST00000544260ENST00000398054MTUS1chr817570722-FGL1chr817726513-0.0048366390.9951633
ENST00000544260ENST00000381840MTUS1chr817570722-FGL1chr817726513-0.0048366390.9951633
ENST00000544260ENST00000381841MTUS1chr817570722-FGL1chr817726513-0.0048366390.9951633
ENST00000544260ENST00000427924MTUS1chr817570722-FGL1chr817726513-0.0048366390.9951633
ENST00000544260ENST00000518650MTUS1chr817570722-FGL1chr817726513-0.0050588650.9949411
ENST00000381861ENST00000398056MTUS1chr817570722-FGL1chr817726513-0.0010308770.9989691
ENST00000381861ENST00000522444MTUS1chr817570722-FGL1chr817726513-0.0010535190.9989465
ENST00000381861ENST00000398054MTUS1chr817570722-FGL1chr817726513-0.0010935760.9989064
ENST00000381861ENST00000381840MTUS1chr817570722-FGL1chr817726513-0.0010935760.9989064
ENST00000381861ENST00000381841MTUS1chr817570722-FGL1chr817726513-0.0010935760.9989064
ENST00000381861ENST00000427924MTUS1chr817570722-FGL1chr817726513-0.0010935760.9989064
ENST00000381861ENST00000518650MTUS1chr817570722-FGL1chr817726513-0.0010203310.9989796
ENST00000519263ENST00000398056MTUS1chr817570722-FGL1chr817726513-0.0002705450.99972945
ENST00000519263ENST00000522444MTUS1chr817570722-FGL1chr817726513-0.0002727810.9997272
ENST00000519263ENST00000398054MTUS1chr817570722-FGL1chr817726513-0.0002818150.9997181
ENST00000519263ENST00000381840MTUS1chr817570722-FGL1chr817726513-0.0002818150.9997181
ENST00000519263ENST00000381841MTUS1chr817570722-FGL1chr817726513-0.0002818150.9997181
ENST00000519263ENST00000427924MTUS1chr817570722-FGL1chr817726513-0.0002818150.9997181
ENST00000519263ENST00000518650MTUS1chr817570722-FGL1chr817726513-0.0003296040.9996704
ENST00000544260ENST00000398056MTUS1chr817570722-FGL1chr817726512-0.0047309240.995269
ENST00000544260ENST00000522444MTUS1chr817570722-FGL1chr817726512-0.0047909750.99520904
ENST00000544260ENST00000398054MTUS1chr817570722-FGL1chr817726512-0.0048366390.9951633
ENST00000544260ENST00000381840MTUS1chr817570722-FGL1chr817726512-0.0048366390.9951633
ENST00000544260ENST00000381841MTUS1chr817570722-FGL1chr817726512-0.0048366390.9951633
ENST00000544260ENST00000427924MTUS1chr817570722-FGL1chr817726512-0.0048366390.9951633
ENST00000544260ENST00000518650MTUS1chr817570722-FGL1chr817726512-0.0050588650.9949411
ENST00000381861ENST00000398056MTUS1chr817570722-FGL1chr817726512-0.0010308770.9989691
ENST00000381861ENST00000522444MTUS1chr817570722-FGL1chr817726512-0.0010535190.9989465
ENST00000381861ENST00000398054MTUS1chr817570722-FGL1chr817726512-0.0010935760.9989064
ENST00000381861ENST00000381840MTUS1chr817570722-FGL1chr817726512-0.0010935760.9989064
ENST00000381861ENST00000381841MTUS1chr817570722-FGL1chr817726512-0.0010935760.9989064
ENST00000381861ENST00000427924MTUS1chr817570722-FGL1chr817726512-0.0010935760.9989064
ENST00000381861ENST00000518650MTUS1chr817570722-FGL1chr817726512-0.0010203310.9989796
ENST00000519263ENST00000398056MTUS1chr817570722-FGL1chr817726512-0.0002705450.99972945
ENST00000519263ENST00000522444MTUS1chr817570722-FGL1chr817726512-0.0002727810.9997272
ENST00000519263ENST00000398054MTUS1chr817570722-FGL1chr817726512-0.0002818150.9997181
ENST00000519263ENST00000381840MTUS1chr817570722-FGL1chr817726512-0.0002818150.9997181
ENST00000519263ENST00000381841MTUS1chr817570722-FGL1chr817726512-0.0002818150.9997181
ENST00000519263ENST00000427924MTUS1chr817570722-FGL1chr817726512-0.0002818150.9997181
ENST00000519263ENST00000518650MTUS1chr817570722-FGL1chr817726512-0.0003296040.9996704

check button Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones.

Get the fusion protein sequences from here.

Fusion protein sequence information is available in the fasta format.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

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Fusion Protein Breakpoint Sequences for MTUS1-FGL1

check button +/-13 AA sequence from the breakpoints of the fusion protein sequences.
HgeneHchrHbpTgeneTchrTbpLength(fusion protein)BP in fusion proteinPeptide
MTUS1chr817570722FGL1chr8177265122635820GPSRKNLFTALNAEDYTLKIDLADFE
MTUS1chr817570722FGL1chr81772651232931GPSRKNLFTALNAEDYTLKIDLADFE
MTUS1chr817570722FGL1chr817726512684121GPSRKNLFTALNAEDYTLKIDLADFE
MTUS1chr817570722FGL1chr8177265132635820GPSRKNLFTALNAEDYTLKIDLADFE
MTUS1chr817570722FGL1chr81772651332931GPSRKNLFTALNAEDYTLKIDLADFE
MTUS1chr817570722FGL1chr817726513684121GPSRKNLFTALNAEDYTLKIDLADFE
MTUS1chr817570723FGL1chr8177265132635820GPSRKNLFTALNAEDYTLKIDLADFE
MTUS1chr817570723FGL1chr81772651332931GPSRKNLFTALNAEDYTLKIDLADFE
MTUS1chr817570723FGL1chr817726513684121GPSRKNLFTALNAEDYTLKIDLADFE

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Potential FusionNeoAntigen Information of MTUS1-FGL1 in HLA I

check button Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.
MTUS1-FGL1_17570722_17726512.msa

check button Potential FusionNeoAntigen Information
* We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5)
Fusion geneHchrHbpTgeneTchrTbpHLA IFusionNeoAntigen peptideBinding scoreImmunogenic scoreNeoantigen start (at BP 13)Neoantigen end (at BP 13)
MTUS1-FGL1chr817570722chr817726512684HLA-B41:01AEDYTLKI0.89210.86621220
MTUS1-FGL1chr817570722chr817726512684HLA-A02:22ALNAEDYTL0.99150.6804918
MTUS1-FGL1chr817570722chr817726512684HLA-A02:67ALNAEDYTL0.98530.6302918
MTUS1-FGL1chr817570722chr817726512684HLA-A02:30ALNAEDYTL0.98530.6302918
MTUS1-FGL1chr817570722chr817726512684HLA-A02:24ALNAEDYTL0.98530.6302918
MTUS1-FGL1chr817570722chr817726512684HLA-A02:60ALNAEDYTL0.98490.6189918
MTUS1-FGL1chr817570722chr817726512684HLA-A02:11ALNAEDYTL0.98450.6708918
MTUS1-FGL1chr817570722chr817726512684HLA-A02:27ALNAEDYTL0.98080.6608918
MTUS1-FGL1chr817570722chr817726512684HLA-A02:13ALNAEDYTL0.97260.7458918
MTUS1-FGL1chr817570722chr817726512684HLA-A02:38ALNAEDYTL0.96730.6779918
MTUS1-FGL1chr817570722chr817726512684HLA-A02:04ALNAEDYTL0.96540.5339918
MTUS1-FGL1chr817570722chr817726512684HLA-A02:19ALNAEDYTL0.9330.5691918
MTUS1-FGL1chr817570722chr817726512684HLA-A02:29ALNAEDYTL0.84960.6318918
MTUS1-FGL1chr817570722chr817726512684HLA-A02:35ALNAEDYTL0.84770.665918
MTUS1-FGL1chr817570722chr817726512684HLA-A02:20ALNAEDYTL0.83260.6366918
MTUS1-FGL1chr817570722chr817726512684HLA-B13:01ALNAEDYTL0.0520.8105918
MTUS1-FGL1chr817570722chr817726512684HLA-B39:08AEDYTLKI0.95430.74741220
MTUS1-FGL1chr817570722chr817726512684HLA-C05:09NAEDYTLKI0.99980.89731120
MTUS1-FGL1chr817570722chr817726512684HLA-C08:15NAEDYTLKI0.99960.93611120
MTUS1-FGL1chr817570722chr817726512684HLA-A02:01ALNAEDYTL0.98530.6302918
MTUS1-FGL1chr817570722chr817726512684HLA-A02:05ALNAEDYTL0.98530.579918
MTUS1-FGL1chr817570722chr817726512684HLA-A02:07ALNAEDYTL0.98440.6203918
MTUS1-FGL1chr817570722chr817726512684HLA-C04:06NAEDYTLKI0.90790.80381120
MTUS1-FGL1chr817570722chr817726512684HLA-C08:13NAEDYTLKI0.64350.95021120
MTUS1-FGL1chr817570722chr817726512684HLA-C08:04NAEDYTLKI0.64350.95021120
MTUS1-FGL1chr817570722chr817726512684HLA-B51:07NAEDYTLKI0.53170.72911120
MTUS1-FGL1chr817570722chr817726512684HLA-C08:03NAEDYTLKI0.17310.96931120
MTUS1-FGL1chr817570722chr817726512684HLA-B39:08AEDYTLKIDL0.93570.79111222
MTUS1-FGL1chr817570722chr817726512684HLA-C05:01NAEDYTLKI0.99980.89731120
MTUS1-FGL1chr817570722chr817726512684HLA-C04:03NAEDYTLKI0.99980.69321120
MTUS1-FGL1chr817570722chr817726512684HLA-C08:02NAEDYTLKI0.99960.93611120
MTUS1-FGL1chr817570722chr817726512684HLA-A02:14ALNAEDYTL0.97450.6678918
MTUS1-FGL1chr817570722chr817726512684HLA-C15:05NAEDYTLKI0.9490.89631120
MTUS1-FGL1chr817570722chr817726512684HLA-C03:02FTALNAEDY0.6930.9631716
MTUS1-FGL1chr817570722chr817726512684HLA-B15:73ALNAEDYTL0.6590.7434918
MTUS1-FGL1chr817570722chr817726512684HLA-B15:30ALNAEDYTL0.59490.6612918
MTUS1-FGL1chr817570722chr817726512684HLA-C08:01NAEDYTLKI0.17310.96931120
MTUS1-FGL1chr817570722chr817726512684HLA-B40:04AEDYTLKIDL0.98880.64051222

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Potential FusionNeoAntigen Information of MTUS1-FGL1 in HLA II

check button Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.
MTUS1-FGL1_17570722_17726512.msa

check button Potential FusionNeoAntigen Information
* We used NetMHCIIpan v4.1 (%rank<0.5).
Fusion geneHchrHbpTgeneTchrTbpHLA IIFusionNeoAntigen peptideNeoantigen start (at BP 13)Neoantigen end (at BP 13)
MTUS1-FGL1chr817570722chr817726512684DRB1-0411SRKNLFTALNAEDYT217
MTUS1-FGL1chr817570722chr817726512684DRB1-0411RKNLFTALNAEDYTL318
MTUS1-FGL1chr817570722chr817726512684DRB1-0411PSRKNLFTALNAEDY116
MTUS1-FGL1chr817570722chr817726512684DRB1-0417SRKNLFTALNAEDYT217
MTUS1-FGL1chr817570722chr817726512684DRB1-0417RKNLFTALNAEDYTL318
MTUS1-FGL1chr817570722chr817726512684DRB1-0424SRKNLFTALNAEDYT217
MTUS1-FGL1chr817570722chr817726512684DRB1-0467SRKNLFTALNAEDYT217
MTUS1-FGL1chr817570722chr817726512684DRB1-0486NAEDYTLKIDLADFE1126
MTUS1-FGL1chr817570722chr817726512684DRB1-0486LNAEDYTLKIDLADF1025
MTUS1-FGL1chr817570722chr817726512684DRB1-0491SRKNLFTALNAEDYT217
MTUS1-FGL1chr817570722chr817726512684DRB1-0491RKNLFTALNAEDYTL318
MTUS1-FGL1chr817570722chr817726512684DRB1-0491PSRKNLFTALNAEDY116
MTUS1-FGL1chr817570722chr817726512684DRB1-0801NAEDYTLKIDLADFE1126
MTUS1-FGL1chr817570722chr817726512684DRB1-0801LNAEDYTLKIDLADF1025
MTUS1-FGL1chr817570722chr817726512684DRB1-0803NAEDYTLKIDLADFE1126
MTUS1-FGL1chr817570722chr817726512684DRB1-0803LNAEDYTLKIDLADF1025
MTUS1-FGL1chr817570722chr817726512684DRB1-0805NAEDYTLKIDLADFE1126
MTUS1-FGL1chr817570722chr817726512684DRB1-0808NAEDYTLKIDLADFE1126
MTUS1-FGL1chr817570722chr817726512684DRB1-0811NAEDYTLKIDLADFE1126
MTUS1-FGL1chr817570722chr817726512684DRB1-0814NAEDYTLKIDLADFE1126
MTUS1-FGL1chr817570722chr817726512684DRB1-0814LNAEDYTLKIDLADF1025
MTUS1-FGL1chr817570722chr817726512684DRB1-0816NAEDYTLKIDLADFE1126
MTUS1-FGL1chr817570722chr817726512684DRB1-0816LNAEDYTLKIDLADF1025
MTUS1-FGL1chr817570722chr817726512684DRB1-0818NAEDYTLKIDLADFE1126
MTUS1-FGL1chr817570722chr817726512684DRB1-0823NAEDYTLKIDLADFE1126
MTUS1-FGL1chr817570722chr817726512684DRB1-0823LNAEDYTLKIDLADF1025
MTUS1-FGL1chr817570722chr817726512684DRB1-0826NAEDYTLKIDLADFE1126
MTUS1-FGL1chr817570722chr817726512684DRB1-0826LNAEDYTLKIDLADF1025
MTUS1-FGL1chr817570722chr817726512684DRB1-0827NAEDYTLKIDLADFE1126
MTUS1-FGL1chr817570722chr817726512684DRB1-0827LNAEDYTLKIDLADF1025
MTUS1-FGL1chr817570722chr817726512684DRB1-0832NAEDYTLKIDLADFE1126
MTUS1-FGL1chr817570722chr817726512684DRB1-0833NAEDYTLKIDLADFE1126
MTUS1-FGL1chr817570722chr817726512684DRB1-0833LNAEDYTLKIDLADF1025
MTUS1-FGL1chr817570722chr817726512684DRB1-0835NAEDYTLKIDLADFE1126
MTUS1-FGL1chr817570722chr817726512684DRB1-0835LNAEDYTLKIDLADF1025
MTUS1-FGL1chr817570722chr817726512684DRB1-0836NAEDYTLKIDLADFE1126
MTUS1-FGL1chr817570722chr817726512684DRB1-0836LNAEDYTLKIDLADF1025
MTUS1-FGL1chr817570722chr817726512684DRB1-0837NAEDYTLKIDLADFE1126
MTUS1-FGL1chr817570722chr817726512684DRB1-0838NAEDYTLKIDLADFE1126
MTUS1-FGL1chr817570722chr817726512684DRB1-0838LNAEDYTLKIDLADF1025
MTUS1-FGL1chr817570722chr817726512684DRB1-0839NAEDYTLKIDLADFE1126
MTUS1-FGL1chr817570722chr817726512684DRB1-0839LNAEDYTLKIDLADF1025
MTUS1-FGL1chr817570722chr817726512684DRB1-1216KNLFTALNAEDYTLK419
MTUS1-FGL1chr817570722chr817726512684DRB1-1216RKNLFTALNAEDYTL318
MTUS1-FGL1chr817570722chr817726512684DRB1-1216SRKNLFTALNAEDYT217
MTUS1-FGL1chr817570722chr817726512684DRB1-1222KNLFTALNAEDYTLK419
MTUS1-FGL1chr817570722chr817726512684DRB1-1222RKNLFTALNAEDYTL318
MTUS1-FGL1chr817570722chr817726512684DRB1-1222SRKNLFTALNAEDYT217
MTUS1-FGL1chr817570722chr817726512684DRB1-1222NLFTALNAEDYTLKI520
MTUS1-FGL1chr817570722chr817726512684DRB1-1313NAEDYTLKIDLADFE1126

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Fusion breakpoint peptide structures of MTUS1-FGL1

check button3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens
* The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA.
File nameBPseqHgeneTgeneHchrHbpTchrTbpAAlen
4980LFTALNAEDYTLKIMTUS1FGL1chr817570722chr817726512684

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of MTUS1-FGL1

check buttonVirtual screening between 25 HLAs (from PDB) and FusionNeoAntigens
* We used Glide to predict the interaction between HLAs and neoantigens.
HLA allelePDB IDFile nameBPseqDocking scoreGlide score
HLA-B14:023BVN4980LFTALNAEDYTLKI-8.62545-8.73885
HLA-B14:023BVN4980LFTALNAEDYTLKI-3.26321-4.29851
HLA-B52:013W394980LFTALNAEDYTLKI-6.23413-6.34753
HLA-B52:013W394980LFTALNAEDYTLKI-4.55402-5.58932
HLA-A24:025HGA4980LFTALNAEDYTLKI-8.62578-8.73918
HLA-A24:025HGA4980LFTALNAEDYTLKI-6.438-7.4733
HLA-B44:053DX84980LFTALNAEDYTLKI-5.68484-5.79824
HLA-B44:053DX84980LFTALNAEDYTLKI-3.64855-4.68385
HLA-A02:016TDR4980LFTALNAEDYTLKI-5.14764-6.18294

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Vaccine Design for the FusionNeoAntigens of MTUS1-FGL1

check button mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is.
Fusion geneHchrHbpTchrTbpStart in +/-13AAEnd in +/-13AAFusionNeoAntigen peptide sequenceFusionNeoAntigen RNA sequence
MTUS1-FGL1chr817570722chr8177265121120NAEDYTLKIATGCAGAAGACTACACTTTAAAAATCG
MTUS1-FGL1chr817570722chr8177265121220AEDYTLKICAGAAGACTACACTTTAAAAATCG
MTUS1-FGL1chr817570722chr8177265121222AEDYTLKIDLCAGAAGACTACACTTTAAAAATCGACCTTG
MTUS1-FGL1chr817570722chr817726512716FTALNAEDYTTACAGCTCTTAATGCAGAAGACTACA
MTUS1-FGL1chr817570722chr817726512918ALNAEDYTLCTCTTAATGCAGAAGACTACACTTTAA

check button mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs.
Fusion geneHchrHbpTchrTbpStart in +/-13AAEnd in +/-13AAFusionNeoAntigen peptideFusionNEoAntigen RNA sequence
MTUS1-FGL1chr817570722chr817726512116PSRKNLFTALNAEDYCTTCGAGAAAAAATTTATTTACAGCTCTTAATGCAGAAGACTACA
MTUS1-FGL1chr817570722chr8177265121025LNAEDYTLKIDLADFTTAATGCAGAAGACTACACTTTAAAAATCGACCTTGCAGATTTTG
MTUS1-FGL1chr817570722chr8177265121126NAEDYTLKIDLADFEATGCAGAAGACTACACTTTAAAAATCGACCTTGCAGATTTTGAAA
MTUS1-FGL1chr817570722chr817726512217SRKNLFTALNAEDYTCGAGAAAAAATTTATTTACAGCTCTTAATGCAGAAGACTACACTT
MTUS1-FGL1chr817570722chr817726512318RKNLFTALNAEDYTLGAAAAAATTTATTTACAGCTCTTAATGCAGAAGACTACACTTTAA
MTUS1-FGL1chr817570722chr817726512419KNLFTALNAEDYTLKAAAATTTATTTACAGCTCTTAATGCAGAAGACTACACTTTAAAAA
MTUS1-FGL1chr817570722chr817726512520NLFTALNAEDYTLKIATTTATTTACAGCTCTTAATGCAGAAGACTACACTTTAAAAATCG

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Information of the samples that have these potential fusion neoantigens of MTUS1-FGL1

check button These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens.
Cancer typeFusion geneHchrHbpHenstTchrTbpTenstSample
LUADMTUS1-FGL1chr817570722ENST00000381861chr817726512ENST00000381840TCGA-55-8094-01A

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Potential target of CAR-T therapy development for MTUS1-FGL1

check button Predicted 3D structure. We used RoseTTAFold.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features


* Minus value of BPloci means that the break point is located before the CDS.
- In-frame and retained 'Transmembrane'.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

check button Subcellular localization prediction of the transmembrane domain retained fusion proteins
* We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image.
HgeneHchrHbpHenstTgeneTchrTbpTenstDeepLoc result

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Related Drugs to MTUS1-FGL1

check button Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDrugSourcePMID

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Related Diseases to MTUS1-FGL1

check button Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDiseaseSourcePMID

check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource