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Fusion Protein:OCLN-ARHGAP26

Fusion Gene and Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: OCLN-ARHGAP26
	FusionPDB ID: 61330
	FusionGDB2.0 ID: 61330
		Hgene	Tgene
	Gene symbol	OCLN	ARHGAP26
	Gene ID	100506658	23092
	Gene name	occludin	Rho GTPase activating protein 26
	Synonyms	BLCPMG\|PPP1R115\|PTORCH1	GRAF\|GRAF1\|OPHN1L\|OPHN1L1
	Cytomap	5q13.2	5q31.3
	Type of gene	protein-coding	protein-coding
	Description	occludinphosphatase 1, regulatory subunit 115tight junction protein occludin	rho GTPase-activating protein 26GTPase regulator associated with focal adhesion kinase pp125(FAK)oligophrenin-1-like protein
	Modification date	20200313	20200313
	UniProtAcc	.	Q9UNA1 Main function of 5'-partner protein: FUNCTION: GTPase-activating protein for RHOA and CDC42.
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000355237, ENST00000396442, ENST00000538151, ENST00000380766, ENST00000514370, ENST00000542132,	ENST00000486650, ENST00000274498, ENST00000378004,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	9 X 6 X 7=378	23 X 27 X 10=6210
	# samples	13	30
	** MAII score	log2(13/378*10)=-1.53987461119262 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(30/6210*10)=-4.37155886261196 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Fusion gene context	PubMed: OCLN [Title/Abstract] AND ARHGAP26 [Title/Abstract] AND fusion [Title/Abstract]
Fusion neoantigen context	PubMed: OCLN [Title/Abstract] AND ARHGAP26 [Title/Abstract] AND neoantigen [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	OCLN(68809936)-ARHGAP26(142393645), # samples:2
Anticipated loss of major functional domain due to fusion event.	OCLN-ARHGAP26 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. OCLN-ARHGAP26 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. OCLN-ARHGAP26 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. OCLN-ARHGAP26 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner

Gene

GO ID

GO term

PubMed ID

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr5:68809936/chr5:142393645)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Retention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here.

Fusion gene breakpoints across OCLN (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across ARHGAP26 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Amino Acid Sequences

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000355237	OCLN	chr5	68809936	+	ENST00000378004	ARHGAP26	chr5	142393645	+	9065	1327	436	2499	687
ENST00000355237	OCLN	chr5	68809936	+	ENST00000274498	ARHGAP26	chr5	142393645	+	6704	1327	436	2664	742
ENST00000396442	OCLN	chr5	68809936	+	ENST00000378004	ARHGAP26	chr5	142393645	+	8808	1070	179	2242	687
ENST00000396442	OCLN	chr5	68809936	+	ENST00000274498	ARHGAP26	chr5	142393645	+	6447	1070	179	2407	742
ENST00000538151	OCLN	chr5	68809936	+	ENST00000378004	ARHGAP26	chr5	142393645	+	7994	256	109	1428	439
ENST00000538151	OCLN	chr5	68809936	+	ENST00000274498	ARHGAP26	chr5	142393645	+	5633	256	109	1593	494
ENST00000355237	OCLN	chr5	68809936	+	ENST00000378004	ARHGAP26	chr5	142393644	+	9065	1327	436	2499	687
ENST00000355237	OCLN	chr5	68809936	+	ENST00000274498	ARHGAP26	chr5	142393644	+	6704	1327	436	2664	742
ENST00000396442	OCLN	chr5	68809936	+	ENST00000378004	ARHGAP26	chr5	142393644	+	8808	1070	179	2242	687
ENST00000396442	OCLN	chr5	68809936	+	ENST00000274498	ARHGAP26	chr5	142393644	+	6447	1070	179	2407	742
ENST00000538151	OCLN	chr5	68809936	+	ENST00000378004	ARHGAP26	chr5	142393644	+	7994	256	109	1428	439
ENST00000538151	OCLN	chr5	68809936	+	ENST00000274498	ARHGAP26	chr5	142393644	+	5633	256	109	1593	494

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000355237	ENST00000378004	OCLN	chr5	68809936	+	ARHGAP26	chr5	142393645	+	0.001757642	0.9982424
ENST00000355237	ENST00000274498	OCLN	chr5	68809936	+	ARHGAP26	chr5	142393645	+	0.002815414	0.9971846
ENST00000396442	ENST00000378004	OCLN	chr5	68809936	+	ARHGAP26	chr5	142393645	+	0.001618151	0.99838185
ENST00000396442	ENST00000274498	OCLN	chr5	68809936	+	ARHGAP26	chr5	142393645	+	0.00261836	0.9973816
ENST00000538151	ENST00000378004	OCLN	chr5	68809936	+	ARHGAP26	chr5	142393645	+	0.000640359	0.99935967
ENST00000538151	ENST00000274498	OCLN	chr5	68809936	+	ARHGAP26	chr5	142393645	+	0.001374532	0.9986255
ENST00000355237	ENST00000378004	OCLN	chr5	68809936	+	ARHGAP26	chr5	142393644	+	0.001757642	0.9982424
ENST00000355237	ENST00000274498	OCLN	chr5	68809936	+	ARHGAP26	chr5	142393644	+	0.002815414	0.9971846
ENST00000396442	ENST00000378004	OCLN	chr5	68809936	+	ARHGAP26	chr5	142393644	+	0.001618151	0.99838185
ENST00000396442	ENST00000274498	OCLN	chr5	68809936	+	ARHGAP26	chr5	142393644	+	0.00261836	0.9973816
ENST00000538151	ENST00000378004	OCLN	chr5	68809936	+	ARHGAP26	chr5	142393644	+	0.000640359	0.99935967
ENST00000538151	ENST00000274498	OCLN	chr5	68809936	+	ARHGAP26	chr5	142393644	+	0.001374532	0.9986255

Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones.

Get the fusion protein sequences from here.

Fusion protein sequence information is available in the fasta format.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

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Fusion Protein Breakpoint Sequences for OCLN-ARHGAP26

+/-13 AA sequence from the breakpoints of the fusion protein sequences.

Hgene	Hchr	Hbp	Tgene	Tchr	Tbp	Length(fusion protein)	BP in fusion protein	Peptide
OCLN	chr5	68809936	ARHGAP26	chr5	142393644	1070	297	HIYDEQPPNVEEWVYNSNKDSQSEGT
OCLN	chr5	68809936	ARHGAP26	chr5	142393644	1327	297	HIYDEQPPNVEEWVYNSNKDSQSEGT
OCLN	chr5	68809936	ARHGAP26	chr5	142393644	256	49	HIYDEQPPNVEEWVYNSNKDSQSEGT
OCLN	chr5	68809936	ARHGAP26	chr5	142393645	1070	297	HIYDEQPPNVEEWVYNSNKDSQSEGT
OCLN	chr5	68809936	ARHGAP26	chr5	142393645	1327	297	HIYDEQPPNVEEWVYNSNKDSQSEGT
OCLN	chr5	68809936	ARHGAP26	chr5	142393645	256	49	HIYDEQPPNVEEWVYNSNKDSQSEGT

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Potential FusionNeoAntigen Information of OCLN-ARHGAP26 in HLA I

Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.

OCLN-ARHGAP26_68809936_142393644.msa

Potential FusionNeoAntigen Information
* We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5)

Fusion gene	Hchr	Hbp	Tgene	Tchr	Tbp	HLA I	FusionNeoAntigen peptide	Binding score	Immunogenic score	Neoantigen start (at BP 13)	Neoantigen end (at BP 13)
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	HLA-B35:01	PPNVEEWVY	0.7118	0.8497	6	15
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	HLA-B35:08	PPNVEEWVY	0.6144	0.8042	6	15
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	HLA-B35:01	QPPNVEEWVY	0.8897	0.9622	5	15
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	HLA-B35:08	QPPNVEEWVY	0.8593	0.9246	5	15
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	HLA-B35:05	QPPNVEEWVY	0.5772	0.71	5	15
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	HLA-B15:31	EQPPNVEEWVY	0.9333	0.7988	4	15
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	HLA-B35:77	PPNVEEWVY	0.7118	0.8497	6	15
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	HLA-B35:77	QPPNVEEWVY	0.8897	0.9622	5	15
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	HLA-B35:23	QPPNVEEWVY	0.8835	0.9624	5	15
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	HLA-B35:24	QPPNVEEWVY	0.7914	0.9478	5	15
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	HLA-B35:20	EQPPNVEEWVY	0.9228	0.89	4	15
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	HLA-B18:08	EQPPNVEEWVY	0.8435	0.6676	4	15

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Potential FusionNeoAntigen Information of OCLN-ARHGAP26 in HLA II

OCLN-ARHGAP26_68809936_142393644.msa

Potential FusionNeoAntigen Information
* We used NetMHCIIpan v4.1 (%rank<0.5).

Fusion gene	Hchr	Hbp	Tgene	Tchr	Tbp	HLA II	FusionNeoAntigen peptide	Neoantigen start (at BP 13)	Neoantigen end (at BP 13)
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB1-0434	VEEWVYNSNKDSQSE	9	24
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB1-0434	EEWVYNSNKDSQSEG	10	25
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB1-0434	EWVYNSNKDSQSEGT	11	26
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB1-0434	NVEEWVYNSNKDSQS	8	23
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB1-0466	VEEWVYNSNKDSQSE	9	24
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB1-0472	EWVYNSNKDSQSEGT	11	26
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB1-0472	VEEWVYNSNKDSQSE	9	24
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB1-0472	EEWVYNSNKDSQSEG	10	25
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB1-0474	EWVYNSNKDSQSEGT	11	26
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB1-0474	VEEWVYNSNKDSQSE	9	24
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB1-0474	EEWVYNSNKDSQSEG	10	25
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB1-1130	VEEWVYNSNKDSQSE	9	24
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB1-1367	VEEWVYNSNKDSQSE	9	24
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB1-1367	NVEEWVYNSNKDSQS	8	23
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB1-1367	EEWVYNSNKDSQSEG	10	25
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB1-1419	VEEWVYNSNKDSQSE	9	24
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB1-1424	VEEWVYNSNKDSQSE	9	24
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB1-1446	VEEWVYNSNKDSQSE	9	24
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB1-1447	VEEWVYNSNKDSQSE	9	24
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0202	VEEWVYNSNKDSQSE	9	24
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0202	NVEEWVYNSNKDSQS	8	23
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0202	EEWVYNSNKDSQSEG	10	25
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0202	PNVEEWVYNSNKDSQ	7	22
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0202	EWVYNSNKDSQSEGT	11	26
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0205	VEEWVYNSNKDSQSE	9	24
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0205	NVEEWVYNSNKDSQS	8	23
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0205	EEWVYNSNKDSQSEG	10	25
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0205	PNVEEWVYNSNKDSQ	7	22
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0205	EWVYNSNKDSQSEGT	11	26
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0209	VEEWVYNSNKDSQSE	9	24
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0209	NVEEWVYNSNKDSQS	8	23
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0210	VEEWVYNSNKDSQSE	9	24
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0210	NVEEWVYNSNKDSQS	8	23
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0210	EEWVYNSNKDSQSEG	10	25
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0210	PNVEEWVYNSNKDSQ	7	22
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0210	EWVYNSNKDSQSEGT	11	26
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0211	VEEWVYNSNKDSQSE	9	24
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0211	NVEEWVYNSNKDSQS	8	23
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0211	EEWVYNSNKDSQSEG	10	25
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0211	PNVEEWVYNSNKDSQ	7	22
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0211	EWVYNSNKDSQSEGT	11	26
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0212	VEEWVYNSNKDSQSE	9	24
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0212	NVEEWVYNSNKDSQS	8	23
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0212	EEWVYNSNKDSQSEG	10	25
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0212	PNVEEWVYNSNKDSQ	7	22
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0212	EWVYNSNKDSQSEGT	11	26
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0213	VEEWVYNSNKDSQSE	9	24
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0213	NVEEWVYNSNKDSQS	8	23
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0213	EEWVYNSNKDSQSEG	10	25
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0213	PNVEEWVYNSNKDSQ	7	22
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0213	EWVYNSNKDSQSEGT	11	26
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0214	VEEWVYNSNKDSQSE	9	24
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0214	NVEEWVYNSNKDSQS	8	23
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0214	EEWVYNSNKDSQSEG	10	25
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0215	VEEWVYNSNKDSQSE	9	24
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0215	NVEEWVYNSNKDSQS	8	23
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0215	EEWVYNSNKDSQSEG	10	25
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0215	PNVEEWVYNSNKDSQ	7	22
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0215	EWVYNSNKDSQSEGT	11	26
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0216	VEEWVYNSNKDSQSE	9	24
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0216	NVEEWVYNSNKDSQS	8	23
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0216	EEWVYNSNKDSQSEG	10	25
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0216	PNVEEWVYNSNKDSQ	7	22
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0217	VEEWVYNSNKDSQSE	9	24
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0217	NVEEWVYNSNKDSQS	8	23
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0217	EEWVYNSNKDSQSEG	10	25
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0217	PNVEEWVYNSNKDSQ	7	22
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0217	EWVYNSNKDSQSEGT	11	26
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0218	VEEWVYNSNKDSQSE	9	24
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0218	NVEEWVYNSNKDSQS	8	23
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0218	EEWVYNSNKDSQSEG	10	25
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0218	PNVEEWVYNSNKDSQ	7	22
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0218	EWVYNSNKDSQSEGT	11	26
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0219	VEEWVYNSNKDSQSE	9	24
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0219	NVEEWVYNSNKDSQS	8	23
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0219	EEWVYNSNKDSQSEG	10	25
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0219	PNVEEWVYNSNKDSQ	7	22
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0219	EWVYNSNKDSQSEGT	11	26
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0220	VEEWVYNSNKDSQSE	9	24
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0220	NVEEWVYNSNKDSQS	8	23
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0220	EEWVYNSNKDSQSEG	10	25
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0220	PNVEEWVYNSNKDSQ	7	22
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0220	EWVYNSNKDSQSEGT	11	26
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0221	VEEWVYNSNKDSQSE	9	24
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0221	NVEEWVYNSNKDSQS	8	23
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0222	VEEWVYNSNKDSQSE	9	24
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0222	NVEEWVYNSNKDSQS	8	23
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0222	EEWVYNSNKDSQSEG	10	25
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0222	PNVEEWVYNSNKDSQ	7	22
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0222	EWVYNSNKDSQSEGT	11	26
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0223	VEEWVYNSNKDSQSE	9	24
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0223	NVEEWVYNSNKDSQS	8	23
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0223	EEWVYNSNKDSQSEG	10	25
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0223	PNVEEWVYNSNKDSQ	7	22
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0223	EWVYNSNKDSQSEGT	11	26
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0225	VEEWVYNSNKDSQSE	9	24
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0225	NVEEWVYNSNKDSQS	8	23
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0225	EEWVYNSNKDSQSEG	10	25
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0225	PNVEEWVYNSNKDSQ	7	22
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0225	EWVYNSNKDSQSEGT	11	26
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	1327	DRB3-0303	VEEWVYNSNKDSQSE	9	24

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Fusion breakpoint peptide structures of OCLN-ARHGAP26

3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens
* The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA.

File name	BPseq	Hgene	Tgene	Hchr	Hbp	Tchr	Tbp	AAlen
6873	PPNVEEWVYNSNKD	OCLN	ARHGAP26	chr5	68809936	chr5	142393644	1327

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of OCLN-ARHGAP26

Virtual screening between 25 HLAs (from PDB) and FusionNeoAntigens
* We used Glide to predict the interaction between HLAs and neoantigens.

HLA allele	PDB ID	File name	BPseq	Docking score	Glide score
HLA-B14:02	3BVN	6873	PPNVEEWVYNSNKD	-7.9962	-8.1096
HLA-B14:02	3BVN	6873	PPNVEEWVYNSNKD	-5.70842	-6.74372
HLA-B52:01	3W39	6873	PPNVEEWVYNSNKD	-6.83737	-6.95077
HLA-B52:01	3W39	6873	PPNVEEWVYNSNKD	-4.4836	-5.5189
HLA-A11:01	4UQ2	6873	PPNVEEWVYNSNKD	-10.0067	-10.1201
HLA-A11:01	4UQ2	6873	PPNVEEWVYNSNKD	-9.03915	-10.0745
HLA-A24:02	5HGA	6873	PPNVEEWVYNSNKD	-6.56204	-6.67544
HLA-A24:02	5HGA	6873	PPNVEEWVYNSNKD	-5.42271	-6.45801
HLA-B44:05	3DX8	6873	PPNVEEWVYNSNKD	-7.85648	-8.89178
HLA-B44:05	3DX8	6873	PPNVEEWVYNSNKD	-5.3978	-5.5112
HLA-A02:01	6TDR	6873	PPNVEEWVYNSNKD	-3.37154	-4.40684

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Vaccine Design for the FusionNeoAntigens of OCLN-ARHGAP26

mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is.

Fusion gene	Hchr	Hbp	Tchr	Tbp	Start in +/-13AA	End in +/-13AA	FusionNeoAntigen peptide sequence	FusionNeoAntigen RNA sequence
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	4	15	EQPPNVEEWVY	GAGCAGCCCCCCAATGTCGAGGAGTGGGTCTAC
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	5	15	QPPNVEEWVY	CAGCCCCCCAATGTCGAGGAGTGGGTCTAC
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	6	15	PPNVEEWVY	CCCCCCAATGTCGAGGAGTGGGTCTAC

mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs.

Fusion gene	Hchr	Hbp	Tchr	Tbp	Start in +/-13AA	End in +/-13AA	FusionNeoAntigen peptide	FusionNEoAntigen RNA sequence
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	10	25	EEWVYNSNKDSQSEG	GAGGAGTGGGTCTACAACTCGAACAAAGACAGCCAGAGTGAAGGG
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	11	26	EWVYNSNKDSQSEGT	GAGTGGGTCTACAACTCGAACAAAGACAGCCAGAGTGAAGGGACT
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	7	22	PNVEEWVYNSNKDSQ	CCCAATGTCGAGGAGTGGGTCTACAACTCGAACAAAGACAGCCAG
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	8	23	NVEEWVYNSNKDSQS	AATGTCGAGGAGTGGGTCTACAACTCGAACAAAGACAGCCAGAGT
OCLN-ARHGAP26	chr5	68809936	chr5	142393644	9	24	VEEWVYNSNKDSQSE	GTCGAGGAGTGGGTCTACAACTCGAACAAAGACAGCCAGAGTGAA

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Information of the samples that have these potential fusion neoantigens of OCLN-ARHGAP26

These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens.

Cancer type	Fusion gene	Hchr	Hbp	Henst	Tchr	Tbp	Tenst	Sample
STAD	OCLN-ARHGAP26	chr5	68809936	ENST00000355237	chr5	142393644	ENST00000274498	TCGA-CD-8528

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Potential target of CAR-T therapy development for OCLN-ARHGAP26

Predicted 3D structure. We used RoseTTAFold.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features

* Minus value of BPloci means that the break point is located before the CDS.

- In-frame and retained 'Transmembrane'.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

OCLN

chr5:68809936

chr5:142393644

ENST00000355237

136_160

297

523.0

Transmembrane

Helical

Hgene

OCLN

chr5:68809936

chr5:142393644

ENST00000355237

171_195

297

523.0

Transmembrane

Helical

Hgene

OCLN

chr5:68809936

chr5:142393644

ENST00000355237

244_265

297

523.0

Transmembrane

Helical

Hgene

OCLN

chr5:68809936

chr5:142393644

ENST00000355237

67_89

297

523.0

Transmembrane

Helical

Hgene

OCLN

chr5:68809936

chr5:142393644

ENST00000396442

136_160

297

523.0

Transmembrane

Helical

Hgene

OCLN

chr5:68809936

chr5:142393644

ENST00000396442

171_195

297

523.0

Transmembrane

Helical

Hgene

OCLN

chr5:68809936

chr5:142393644

ENST00000396442

244_265

297

523.0

Transmembrane

Helical

Hgene

OCLN

chr5:68809936

chr5:142393644

ENST00000396442

67_89

297

523.0

Transmembrane

Helical

Hgene

OCLN

chr5:68809936

chr5:142393645

ENST00000355237

136_160

297

523.0

Transmembrane

Helical

Hgene

OCLN

chr5:68809936

chr5:142393645

ENST00000355237

171_195

297

523.0

Transmembrane

Helical

Hgene

OCLN

chr5:68809936

chr5:142393645

ENST00000355237

244_265

297

523.0

Transmembrane

Helical

Hgene

OCLN

chr5:68809936

chr5:142393645

ENST00000355237

67_89

297

523.0

Transmembrane

Helical

Hgene

OCLN

chr5:68809936

chr5:142393645

ENST00000396442

136_160

297

523.0

Transmembrane

Helical

Hgene

OCLN

chr5:68809936

chr5:142393645

ENST00000396442

171_195

297

523.0

Transmembrane

Helical

Hgene

OCLN

chr5:68809936

chr5:142393645

ENST00000396442

244_265

297

523.0

Transmembrane

Helical

Hgene

OCLN

chr5:68809936

chr5:142393645

ENST00000396442

67_89

297

523.0

Transmembrane

Helical

Subcellular localization prediction of the transmembrane domain retained fusion proteins
* We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image.

Hgene

Hchr

Hbp

Henst

Tgene

Tchr

Tbp

Tenst

DeepLoc result

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Related Drugs to OCLN-ARHGAP26

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

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Related Diseases to OCLN-ARHGAP26

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner	Gene	Disease ID	Disease name	# pubmeds	Source
Tgene	ARHGAP26	C0033975	Psychotic Disorders	1	PSYGENET

	Fusion Gene and Fusion Protein Summary
	Fusion Amino Acid Sequences (multiple BPs and multiple gene isoforms)
	Fusion Protein Breakpoint Sequences - (for the Screening of the FusionNeoAntigens)
	Potential FusionNeoAntigens in HLA I - (netMHCpan v4.1 + deepHLApan v1.1)
	Potential FusionNeoAntigens in HLA II - (netMHCIIpan v4.1)
	Fusion Breakpoint 14 AA Peptide Structure - (RoseTTAFold)
	Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D - (Glide)
	Vaccine Design for the FusionNeoAntigens (RNA/protein sequences)
	Potential target of CAR-T therapy development
	Information on the samples that have these potential fusion neoantigens
	Fusion Protein Targeting Drugs - (Manual Curation)
	Fusion Protein Related diseases - (Manual Curation)