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Fusion Protein:PRCC-BTF3L4

Fusion Gene and Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: PRCC-BTF3L4
	FusionPDB ID: 68399
	FusionGDB2.0 ID: 68399
		Hgene	Tgene
	Gene symbol	PRCC	BTF3L4
	Gene ID	5546	91408
	Gene name	proline rich mitotic checkpoint control factor	basic transcription factor 3 like 4
	Synonyms	RCCP1\|TPRC	-
	Cytomap	1q23.1	1p32.3
	Type of gene	protein-coding	protein-coding
	Description	proline-rich protein PRCCPRCC, proline rich mitotic checkpoint control factorpapillary renal cell carcinoma (translocation-associated)papillary renal cell carcinoma translocation-associated gene protein	transcription factor BTF3 homolog 4
	Modification date	20200313	20200313
	UniProtAcc	Q92733 Main function of 5'-partner protein: FUNCTION: May regulate cell cycle progression through interaction with MAD2L2. {ECO:0000269\|PubMed:11717438}.	Q96K17 Main function of 5'-partner protein:
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000271526, ENST00000353233, ENST00000491853,	ENST00000533836, ENST00000472944, ENST00000484036, ENST00000489308, ENST00000313334,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	12 X 6 X 10=720	7 X 8 X 4=224
	# samples	14	8
	** MAII score	log2(14/720*10)=-2.36257007938471 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(8/224*10)=-1.48542682717024 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Fusion gene context	PubMed: PRCC [Title/Abstract] AND BTF3L4 [Title/Abstract] AND fusion [Title/Abstract]
Fusion neoantigen context	PubMed: PRCC [Title/Abstract] AND BTF3L4 [Title/Abstract] AND neoantigen [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	PRCC(156738031)-BTF3L4(52549011), # samples:1
Anticipated loss of major functional domain due to fusion event.	PRCC-BTF3L4 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. PRCC-BTF3L4 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. PRCC-BTF3L4 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. PRCC-BTF3L4 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	PRCC	GO:0007093	mitotic cell cycle checkpoint	11717438

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr1:156738031/chr1:52549011)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Retention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here.

Fusion gene breakpoints across PRCC (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across BTF3L4 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Amino Acid Sequences

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst

Tenst

Hgene

Hchr

Hbp

Hstrand

Tgene

Tchr

Tbp

Tstrand

Seq length
(transcript)

BP loci
(transcript)

Predicted start
(transcript)

Predicted stop
(transcript)

Seq length
(amino acids)

ENST00000271526

PRCC

chr1

156738031

ENST00000313334

BTF3L4

chr1

52549011

5007

740

272

1048

258

ENST00000353233

PRCC

chr1

156738031

ENST00000313334

BTF3L4

chr1

52549011

4953

686

218

994

258

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000271526	ENST00000313334	PRCC	chr1	156738031	+	BTF3L4	chr1	52549011	+	0.002132481	0.99786747
ENST00000353233	ENST00000313334	PRCC	chr1	156738031	+	BTF3L4	chr1	52549011	+	0.002164329	0.9978357

Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones.

Get the fusion protein sequences from here.

Fusion protein sequence information is available in the fasta format.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

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Fusion Protein Breakpoint Sequences for PRCC-BTF3L4

+/-13 AA sequence from the breakpoints of the fusion protein sequences.

Hgene	Hchr	Hbp	Tgene	Tchr	Tbp	Length(fusion protein)	BP in fusion protein	Peptide
PRCC	chr1	156738031	BTF3L4	chr1	52549011	686	62	LLPPPPQMLAPAFPPPLLLPPPTGDP
PRCC	chr1	156738031	BTF3L4	chr1	52549011	740	62	LLPPPPQMLAPAFPPPLLLPPPTGDP

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Potential FusionNeoAntigen Information of PRCC-BTF3L4 in HLA I

Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.

PRCC-BTF3L4_156738031_52549011.msa

Potential FusionNeoAntigen Information
* We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5)

Fusion gene	Hchr	Hbp	Tgene	Tchr	Tbp	HLA I	FusionNeoAntigen peptide	Binding score	Immunogenic score	Neoantigen start (at BP 13)	Neoantigen end (at BP 13)
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B07:02	APAFPPPL	0.9996	0.6393	9	17
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B07:05	APAFPPPL	0.9995	0.5488	9	17
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B82:01	APAFPPPL	0.7738	0.5337	9	17
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B07:10	APAFPPPLL	0.9991	0.7509	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B07:02	APAFPPPLL	0.999	0.7048	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B07:05	APAFPPPLL	0.9989	0.5707	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B56:01	FPPPLLLPP	0.9843	0.6517	12	21
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B35:03	APAFPPPLL	0.9803	0.9572	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B35:01	APAFPPPLL	0.9695	0.9577	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B35:08	APAFPPPLL	0.9592	0.9516	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B52:01	PAFPPPLLL	0.9203	0.809	10	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B35:04	APAFPPPLL	0.9113	0.9881	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B35:02	APAFPPPLL	0.9113	0.9881	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B51:02	APAFPPPLL	0.9022	0.5594	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B53:01	APAFPPPLL	0.8839	0.5018	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B35:05	APAFPPPLL	0.8824	0.8866	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B51:01	APAFPPPLL	0.8255	0.5697	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B15:10	APAFPPPLL	0.7506	0.7165	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B15:16	PAFPPPLLL	0.7071	0.5945	10	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B82:01	APAFPPPLL	0.38	0.5135	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-A02:11	MLAPAFPPPL	0.9962	0.756	7	17
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-A02:04	MLAPAFPPPL	0.9941	0.9007	7	17
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-A02:17	MLAPAFPPPL	0.9928	0.8219	7	17
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B07:10	APAFPPPLLL	0.9925	0.7112	9	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B51:01	LAPAFPPPLL	0.9921	0.8589	8	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B07:02	APAFPPPLLL	0.9903	0.6648	9	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B51:02	LAPAFPPPLL	0.9853	0.766	8	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-A02:19	MLAPAFPPPL	0.9839	0.6144	7	17
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B35:03	LAPAFPPPLL	0.9751	0.9284	8	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B35:04	LAPAFPPPLL	0.945	0.9825	8	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B35:02	LAPAFPPPLL	0.945	0.9825	8	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B35:03	APAFPPPLLL	0.9299	0.9277	9	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B81:01	LAPAFPPPLL	0.8901	0.5732	8	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B07:05	LAPAFPPPLL	0.8002	0.5716	8	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B35:04	APAFPPPLLL	0.7897	0.9801	9	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B35:02	APAFPPPLLL	0.7897	0.9801	9	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B07:02	LAPAFPPPLL	0.7879	0.5878	8	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B15:16	APAFPPPLLL	0.7199	0.8106	9	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B82:01	LAPAFPPPLL	0.7138	0.5943	8	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B52:01	APAFPPPLLL	0.6971	0.9558	9	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B15:17	APAFPPPLLL	0.6912	0.9423	9	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-A02:22	MLAPAFPPPLL	0.9989	0.7113	7	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B57:03	LAPAFPPPLLL	0.9987	0.9925	8	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-A02:04	MLAPAFPPPLL	0.9983	0.8571	7	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-A02:11	MLAPAFPPPLL	0.9981	0.6917	7	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-A02:13	MLAPAFPPPLL	0.998	0.8078	7	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-A02:24	MLAPAFPPPLL	0.9979	0.6511	7	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-A02:30	MLAPAFPPPLL	0.9979	0.6511	7	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-A02:67	MLAPAFPPPLL	0.9979	0.6511	7	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-A02:17	MLAPAFPPPLL	0.9979	0.8213	7	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-A02:60	MLAPAFPPPLL	0.9979	0.6513	7	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-A02:27	MLAPAFPPPLL	0.9974	0.7266	7	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-A02:38	MLAPAFPPPLL	0.9967	0.7219	7	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-A02:35	MLAPAFPPPLL	0.9961	0.6619	7	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-A02:16	MLAPAFPPPLL	0.9951	0.5779	7	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-A02:29	MLAPAFPPPLL	0.9943	0.656	7	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B15:17	LAPAFPPPLLL	0.992	0.9272	8	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B15:16	LAPAFPPPLLL	0.992	0.8657	8	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-A02:20	MLAPAFPPPLL	0.9851	0.6565	7	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-A02:19	MLAPAFPPPLL	0.9762	0.5999	7	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B51:02	MLAPAFPPPLL	0.9632	0.6485	7	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B35:03	MLAPAFPPPLL	0.9608	0.9536	7	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B35:02	MLAPAFPPPLL	0.9115	0.9828	7	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B35:04	MLAPAFPPPLL	0.9115	0.9828	7	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B07:05	MLAPAFPPPLL	0.8573	0.5219	7	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B07:12	APAFPPPL	0.9984	0.8094	9	17
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C15:06	AFPPPLLL	0.9966	0.9728	11	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C03:07	AFPPPLLL	0.9956	0.9937	11	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C07:29	AFPPPLLL	0.989	0.9771	11	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C04:10	AFPPPLLL	0.9838	0.9033	11	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C04:07	AFPPPLLL	0.9837	0.9054	11	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C04:14	AFPPPLLL	0.9811	0.9567	11	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C07:13	AFPPPLLL	0.9804	0.9693	11	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B07:04	APAFPPPL	0.9757	0.6662	9	17
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C01:17	AFPPPLLL	0.9713	0.9607	11	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B42:02	APAFPPPL	0.967	0.7924	9	17
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C01:30	AFPPPLLL	0.9597	0.9789	11	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B42:01	APAFPPPL	0.959	0.7817	9	17
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B56:04	APAFPPPL	0.9143	0.577	9	17
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B39:10	APAFPPPL	0.7616	0.9863	9	17
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C03:08	LAPAFPPPL	0.999	0.9376	8	17
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B07:12	APAFPPPLL	0.9988	0.8334	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C03:19	LAPAFPPPL	0.9988	0.9956	8	17
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C03:07	LAPAFPPPL	0.9984	0.9928	8	17
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B54:01	FPPPLLLPP	0.9982	0.7893	12	21
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C12:12	LAPAFPPPL	0.997	0.9839	8	17
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C03:08	PAFPPPLLL	0.9961	0.9181	10	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C06:03	LAPAFPPPL	0.9953	0.9983	8	17
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C03:07	PAFPPPLLL	0.9945	0.9915	10	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C03:19	PAFPPPLLL	0.9912	0.9931	10	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C08:13	LAPAFPPPL	0.9899	0.9947	8	17
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C08:04	LAPAFPPPL	0.9899	0.9947	8	17
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C15:04	PAFPPPLLL	0.9893	0.9408	10	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B78:01	FPPPLLLPP	0.9735	0.6295	12	21
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C15:06	PAFPPPLLL	0.973	0.96	10	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B56:04	APAFPPPLL	0.9577	0.5049	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C04:06	PAFPPPLLL	0.9325	0.9483	10	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B07:04	APAFPPPLL	0.9295	0.7112	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B51:07	PAFPPPLLL	0.9276	0.7026	10	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C08:03	LAPAFPPPL	0.9123	0.9979	8	17
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B35:12	APAFPPPLL	0.9113	0.9881	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B39:10	APAFPPPLL	0.8462	0.9879	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B39:09	APAFPPPLL	0.8449	0.8879	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B51:07	APAFPPPLL	0.8436	0.8415	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C01:30	LAPAFPPPL	0.8254	0.9834	8	17
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B78:01	APAFPPPLL	0.8178	0.6936	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B14:03	APAFPPPLL	0.8008	0.9637	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C12:12	PAFPPPLLL	0.7711	0.9664	10	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B39:08	APAFPPPLL	0.7503	0.9726	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C06:03	PAFPPPLLL	0.7231	0.9974	10	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C12:04	PAFPPPLLL	0.7215	0.9977	10	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C08:04	PAFPPPLLL	0.679	0.987	10	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C08:13	PAFPPPLLL	0.679	0.987	10	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C08:03	APAFPPPLL	0.6762	0.9947	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B54:01	APAFPPPLL	0.6748	0.5246	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C02:06	PAFPPPLLL	0.664	0.9681	10	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B42:02	APAFPPPLL	0.6425	0.7632	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B51:08	APAFPPPLL	0.6408	0.6247	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B42:01	APAFPPPLL	0.5938	0.7569	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C01:17	LAPAFPPPL	0.548	0.9861	8	17
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C03:14	PAFPPPLLL	0.5101	0.9929	10	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C08:03	PAFPPPLLL	0.4872	0.9926	10	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C07:19	PAFPPPLLL	0.4828	0.8654	10	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C04:14	APAFPPPLL	0.4155	0.9335	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C04:10	APAFPPPLL	0.074	0.9135	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C04:07	APAFPPPLL	0.071	0.9311	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C01:30	LAPAFPPPLL	0.9928	0.983	8	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B07:12	APAFPPPLLL	0.992	0.8	9	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C01:17	LAPAFPPPLL	0.9781	0.982	8	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C03:08	APAFPPPLLL	0.9768	0.9279	9	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C03:19	APAFPPPLLL	0.9751	0.9935	9	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C02:06	APAFPPPLLL	0.975	0.9769	9	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B07:04	APAFPPPLLL	0.9671	0.6602	9	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C01:17	MLAPAFPPPL	0.9616	0.9896	7	17
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C03:07	APAFPPPLLL	0.9572	0.9912	9	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B51:08	LAPAFPPPLL	0.9553	0.8457	8	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C12:12	APAFPPPLLL	0.9471	0.9675	9	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B35:12	LAPAFPPPLL	0.945	0.9825	8	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B56:04	LAPAFPPPLL	0.9385	0.681	8	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C12:04	APAFPPPLLL	0.9183	0.998	9	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C06:03	APAFPPPLLL	0.9039	0.9976	9	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C15:06	APAFPPPLLL	0.9029	0.9717	9	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C15:04	APAFPPPLLL	0.8933	0.961	9	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C04:06	APAFPPPLLL	0.8737	0.9744	9	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B07:04	LAPAFPPPLL	0.8495	0.6258	8	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B07:12	LAPAFPPPLL	0.8372	0.7642	8	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B42:02	APAFPPPLLL	0.8198	0.7391	9	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B39:10	APAFPPPLLL	0.8161	0.984	9	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C03:14	APAFPPPLLL	0.8129	0.993	9	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B42:01	LAPAFPPPLL	0.8067	0.7765	8	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C08:04	APAFPPPLLL	0.7917	0.9926	9	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C08:13	APAFPPPLLL	0.7917	0.9926	9	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B35:12	APAFPPPLLL	0.7897	0.9801	9	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B42:01	APAFPPPLLL	0.7653	0.7344	9	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C08:03	APAFPPPLLL	0.7599	0.9946	9	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B39:10	LAPAFPPPLL	0.7093	0.988	8	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B51:07	APAFPPPLLL	0.6851	0.8114	9	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B78:01	APAFPPPLLL	0.6458	0.6555	9	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B51:08	APAFPPPLLL	0.5733	0.5038	9	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C03:19	LAPAFPPPLLL	0.9995	0.9938	8	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C03:08	LAPAFPPPLLL	0.9994	0.9058	8	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C03:07	LAPAFPPPLLL	0.9994	0.9884	8	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C15:06	LAPAFPPPLLL	0.9993	0.9705	8	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-A02:02	MLAPAFPPPLL	0.999	0.6703	7	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C01:30	LAPAFPPPLLL	0.9988	0.9816	8	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C15:04	LAPAFPPPLLL	0.9987	0.9725	8	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C01:17	LAPAFPPPLLL	0.9987	0.9779	8	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-A02:07	MLAPAFPPPLL	0.9982	0.7092	7	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-A02:01	MLAPAFPPPLL	0.9979	0.6511	7	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C02:06	LAPAFPPPLLL	0.9963	0.99	8	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B42:01	MLAPAFPPPLL	0.9678	0.9528	7	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B07:12	MLAPAFPPPLL	0.9119	0.6649	7	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B35:12	MLAPAFPPPLL	0.9115	0.9828	7	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B56:04	MLAPAFPPPLL	0.8358	0.6783	7	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B39:10	MLAPAFPPPLL	0.7374	0.9852	7	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B07:22	APAFPPPL	0.9996	0.6393	9	17
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B07:09	APAFPPPL	0.9995	0.6147	9	17
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C03:05	AFPPPLLL	0.9966	0.9776	11	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C15:05	AFPPPLLL	0.996	0.9629	11	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C04:04	AFPPPLLL	0.9875	0.9677	11	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C03:67	AFPPPLLL	0.9875	0.9895	11	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C06:06	AFPPPLLL	0.9848	0.9958	11	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C04:01	AFPPPLLL	0.9837	0.9054	11	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B56:05	APAFPPPL	0.9805	0.529	9	17
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B55:04	APAFPPPL	0.9797	0.5087	9	17
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C18:01	AFPPPLLL	0.9767	0.9145	11	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B07:26	APAFPPPL	0.9679	0.6772	9	17
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C07:04	AFPPPLLL	0.9677	0.945	11	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C14:03	AFPPPLLL	0.9646	0.981	11	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C14:02	AFPPPLLL	0.9646	0.981	11	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C01:02	AFPPPLLL	0.9645	0.9602	11	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C17:01	AFPPPLLL	0.9608	0.92	11	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C01:03	AFPPPLLL	0.9584	0.9515	11	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B56:02	APAFPPPL	0.9143	0.577	9	17
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B67:01	APAFPPPL	0.7948	0.9771	9	17
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B82:02	APAFPPPL	0.7738	0.5337	9	17
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B07:13	APAFPPPLL	0.9995	0.9344	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B48:05	APAFPPPLL	0.9991	0.5502	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C03:03	LAPAFPPPL	0.999	0.9948	8	17
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C03:04	LAPAFPPPL	0.999	0.9948	8	17
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B07:22	APAFPPPLL	0.999	0.7048	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B07:09	APAFPPPLL	0.9989	0.6641	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C03:05	LAPAFPPPL	0.9987	0.9599	8	17
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C03:17	LAPAFPPPL	0.9986	0.977	8	17
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C03:67	LAPAFPPPL	0.9984	0.9917	8	17
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C03:04	PAFPPPLLL	0.9974	0.9922	10	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C03:03	PAFPPPLLL	0.9974	0.9922	10	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C12:03	LAPAFPPPL	0.9962	0.9955	8	17
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C03:67	PAFPPPLLL	0.995	0.9851	10	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C03:05	PAFPPPLLL	0.9938	0.9576	10	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C03:06	LAPAFPPPL	0.9924	0.9956	8	17
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C16:04	LAPAFPPPL	0.9912	0.9965	8	17
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C03:17	PAFPPPLLL	0.9899	0.9793	10	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C15:09	PAFPPPLLL	0.9893	0.9408	10	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C16:01	LAPAFPPPL	0.9841	0.9953	8	17
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B35:13	LAPAFPPPL	0.9825	0.951	8	17
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B35:22	LAPAFPPPL	0.9752	0.6001	8	17
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B35:13	APAFPPPLL	0.9737	0.9592	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C15:02	PAFPPPLLL	0.9708	0.9458	10	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C15:05	PAFPPPLLL	0.9698	0.9418	10	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B35:77	APAFPPPLL	0.9695	0.9577	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B35:23	APAFPPPLL	0.9676	0.9548	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B56:05	FPPPLLLPP	0.966	0.6289	12	21
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B56:02	APAFPPPLL	0.9577	0.5049	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B55:02	FPPPLLLPP	0.956	0.7367	12	21
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B78:02	FPPPLLLPP	0.9424	0.6414	12	21
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B56:05	APAFPPPLL	0.9246	0.5393	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C04:04	PAFPPPLLL	0.9193	0.945	10	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C08:01	LAPAFPPPL	0.9123	0.9979	8	17
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B35:09	APAFPPPLL	0.9113	0.9881	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B35:17	APAFPPPLL	0.8899	0.9354	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B35:30	APAFPPPLL	0.8899	0.9354	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B35:13	PAFPPPLLL	0.8849	0.9117	10	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C03:06	PAFPPPLLL	0.869	0.9925	10	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B67:01	APAFPPPLL	0.8436	0.9633	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B51:09	APAFPPPLL	0.8392	0.6112	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B39:02	APAFPPPLL	0.8371	0.9917	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B78:02	APAFPPPLL	0.8341	0.8659	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B07:26	APAFPPPLL	0.8286	0.6519	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C12:03	PAFPPPLLL	0.7951	0.9928	10	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B07:13	LAPAFPPPL	0.7871	0.9143	8	17
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B35:11	APAFPPPLL	0.7861	0.9599	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B51:14	APAFPPPLL	0.784	0.5379	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B51:06	APAFPPPLL	0.7742	0.5246	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B53:02	APAFPPPLL	0.7234	0.5092	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B39:11	APAFPPPLL	0.72	0.9544	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C16:02	PAFPPPLLL	0.7134	0.9969	10	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C17:01	LAPAFPPPL	0.6894	0.9886	8	17
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C08:01	APAFPPPLL	0.6762	0.9947	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C16:04	PAFPPPLLL	0.6311	0.9926	10	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C12:02	PAFPPPLLL	0.6165	0.9873	10	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B51:21	APAFPPPLL	0.6133	0.5523	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B07:13	PAFPPPLLL	0.5902	0.88	10	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C02:02	PAFPPPLLL	0.5838	0.9833	10	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C02:10	PAFPPPLLL	0.5838	0.9833	10	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C17:01	PAFPPPLLL	0.5694	0.8438	10	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B15:09	APAFPPPLL	0.5625	0.8053	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C01:02	LAPAFPPPL	0.5252	0.9851	8	17
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C08:01	PAFPPPLLL	0.4872	0.9926	10	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C07:01	PAFPPPLLL	0.4761	0.7813	10	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C04:04	APAFPPPLL	0.4609	0.9736	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C01:03	LAPAFPPPL	0.3809	0.9772	8	17
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B82:02	APAFPPPLL	0.38	0.5135	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C18:01	APAFPPPLL	0.0853	0.93	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C04:01	APAFPPPLL	0.071	0.9311	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B35:43	APAFPPPLL	0.0234	0.9555	9	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B07:13	APAFPPPLLL	0.9933	0.9251	9	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B07:22	APAFPPPLLL	0.9903	0.6648	9	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B07:09	APAFPPPLLL	0.99	0.6226	9	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B51:14	LAPAFPPPLL	0.9897	0.7942	8	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B51:09	LAPAFPPPLL	0.985	0.8224	8	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C03:05	APAFPPPLLL	0.982	0.9523	9	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B51:06	LAPAFPPPLL	0.9816	0.8238	8	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C03:17	APAFPPPLLL	0.9806	0.9793	9	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B35:22	LAPAFPPPLL	0.9797	0.6274	8	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C01:02	LAPAFPPPLL	0.9792	0.9816	8	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C01:03	LAPAFPPPLL	0.9788	0.9778	8	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B51:29	LAPAFPPPLL	0.9783	0.6507	8	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C01:02	MLAPAFPPPL	0.9764	0.9891	7	17
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C03:04	APAFPPPLLL	0.9731	0.9924	9	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C03:03	APAFPPPLLL	0.9731	0.9924	9	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B55:04	LAPAFPPPLL	0.9706	0.5927	8	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C02:02	APAFPPPLLL	0.9616	0.9891	9	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C02:10	APAFPPPLLL	0.9616	0.9891	9	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B07:26	LAPAFPPPLL	0.9513	0.6291	8	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C12:03	APAFPPPLLL	0.9485	0.9931	9	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B35:09	LAPAFPPPLL	0.945	0.9825	8	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B51:05	LAPAFPPPLL	0.9419	0.6764	8	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B56:02	LAPAFPPPLL	0.9385	0.681	8	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C16:04	APAFPPPLLL	0.9269	0.9931	9	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B35:13	APAFPPPLLL	0.9151	0.931	9	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B07:26	APAFPPPLLL	0.9133	0.5684	9	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B58:06	APAFPPPLLL	0.898	0.9441	9	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C12:02	APAFPPPLLL	0.8935	0.9882	9	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C15:09	APAFPPPLLL	0.8933	0.961	9	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C16:02	APAFPPPLLL	0.8888	0.9971	9	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C15:02	APAFPPPLLL	0.8805	0.9651	9	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C15:05	APAFPPPLLL	0.8754	0.968	9	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C03:06	APAFPPPLLL	0.8431	0.9932	9	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C17:01	APAFPPPLLL	0.8117	0.9579	9	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B67:01	APAFPPPLLL	0.8024	0.928	9	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B35:09	APAFPPPLLL	0.7897	0.9801	9	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B07:22	LAPAFPPPLL	0.7879	0.5878	8	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C08:01	APAFPPPLLL	0.7599	0.9946	9	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B67:01	LAPAFPPPLL	0.7264	0.8569	8	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B82:02	LAPAFPPPLL	0.7138	0.5943	8	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B51:09	APAFPPPLLL	0.681	0.5181	9	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B40:21	APAFPPPLLL	0.3352	0.5134	9	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C03:03	LAPAFPPPLLL	0.9996	0.9926	8	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C03:04	LAPAFPPPLLL	0.9996	0.9926	8	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C03:17	LAPAFPPPLLL	0.9995	0.9823	8	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C03:05	LAPAFPPPLLL	0.9995	0.9414	8	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C15:02	LAPAFPPPLLL	0.9994	0.9737	8	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C15:05	LAPAFPPPLLL	0.9994	0.9749	8	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-A02:03	MLAPAFPPPLL	0.9988	0.7944	7	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C15:09	LAPAFPPPLLL	0.9987	0.9725	8	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C01:02	LAPAFPPPLLL	0.9981	0.9777	8	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C01:03	LAPAFPPPLLL	0.9976	0.9788	8	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C03:06	LAPAFPPPLLL	0.9959	0.9932	8	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-C17:01	LAPAFPPPLLL	0.9847	0.9914	8	19
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B55:04	MLAPAFPPPLL	0.976	0.5948	7	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B35:22	MLAPAFPPPLL	0.9637	0.5525	7	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B35:09	MLAPAFPPPLL	0.9115	0.9828	7	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B51:06	MLAPAFPPPLL	0.8951	0.7585	7	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B56:02	MLAPAFPPPLL	0.8358	0.6783	7	18
PRCC-BTF3L4	chr1	156738031	chr1	52549011	740	HLA-B67:01	MLAPAFPPPLL	0.8076	0.8386	7	18

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Potential FusionNeoAntigen Information of PRCC-BTF3L4 in HLA II

Potential FusionNeoAntigen Information
* We used NetMHCIIpan v4.1 (%rank<0.5).

Fusion gene

Hchr

Hbp

Tgene

Tchr

Tbp

HLA II

FusionNeoAntigen peptide

Neoantigen start (at BP 13)

Neoantigen end (at BP 13)

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Fusion breakpoint peptide structures of PRCC-BTF3L4

3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens
* The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA.

File name	BPseq	Hgene	Tgene	Hchr	Hbp	Tchr	Tbp	AAlen
7413	QMLAPAFPPPLLLP	PRCC	BTF3L4	chr1	156738031	chr1	52549011	740

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of PRCC-BTF3L4

Virtual screening between 25 HLAs (from PDB) and FusionNeoAntigens
* We used Glide to predict the interaction between HLAs and neoantigens.

HLA allele	PDB ID	File name	BPseq	Docking score	Glide score
HLA-B14:02	3BVN	7413	QMLAPAFPPPLLLP	-7.9962	-8.1096
HLA-B14:02	3BVN	7413	QMLAPAFPPPLLLP	-5.70842	-6.74372
HLA-B52:01	3W39	7413	QMLAPAFPPPLLLP	-6.83737	-6.95077
HLA-B52:01	3W39	7413	QMLAPAFPPPLLLP	-4.4836	-5.5189
HLA-A11:01	4UQ2	7413	QMLAPAFPPPLLLP	-10.0067	-10.1201
HLA-A11:01	4UQ2	7413	QMLAPAFPPPLLLP	-9.03915	-10.0745
HLA-A24:02	5HGA	7413	QMLAPAFPPPLLLP	-6.56204	-6.67544
HLA-A24:02	5HGA	7413	QMLAPAFPPPLLLP	-5.42271	-6.45801
HLA-B44:05	3DX8	7413	QMLAPAFPPPLLLP	-7.85648	-8.89178
HLA-B44:05	3DX8	7413	QMLAPAFPPPLLLP	-5.3978	-5.5112
HLA-A02:01	6TDR	7413	QMLAPAFPPPLLLP	-3.37154	-4.40684

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Vaccine Design for the FusionNeoAntigens of PRCC-BTF3L4

mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is.

Fusion gene	Hchr	Hbp	Tchr	Tbp	Start in +/-13AA	End in +/-13AA	FusionNeoAntigen peptide sequence	FusionNeoAntigen RNA sequence
PRCC-BTF3L4	chr1	156738031	chr1	52549011	10	19	PAFPPPLLL	AAGGGAGATGTGAACATGATTAAAGAT
PRCC-BTF3L4	chr1	156738031	chr1	52549011	11	19	AFPPPLLL	GGAGATGTGAACATGATTAAAGAT
PRCC-BTF3L4	chr1	156738031	chr1	52549011	12	21	FPPPLLLPP	GATGTGAACATGATTAAAGATGATGGG
PRCC-BTF3L4	chr1	156738031	chr1	52549011	7	17	MLAPAFPPPL	GAGTTGCATAAGGGAGATGTGAACATGATT
PRCC-BTF3L4	chr1	156738031	chr1	52549011	7	18	MLAPAFPPPLL	GAGTTGCATAAGGGAGATGTGAACATGATTAAA
PRCC-BTF3L4	chr1	156738031	chr1	52549011	8	17	LAPAFPPPL	TTGCATAAGGGAGATGTGAACATGATT
PRCC-BTF3L4	chr1	156738031	chr1	52549011	8	18	LAPAFPPPLL	TTGCATAAGGGAGATGTGAACATGATTAAA
PRCC-BTF3L4	chr1	156738031	chr1	52549011	8	19	LAPAFPPPLLL	TTGCATAAGGGAGATGTGAACATGATTAAAGAT
PRCC-BTF3L4	chr1	156738031	chr1	52549011	9	17	APAFPPPL	CATAAGGGAGATGTGAACATGATT
PRCC-BTF3L4	chr1	156738031	chr1	52549011	9	18	APAFPPPLL	CATAAGGGAGATGTGAACATGATTAAA
PRCC-BTF3L4	chr1	156738031	chr1	52549011	9	19	APAFPPPLLL	CATAAGGGAGATGTGAACATGATTAAAGAT

mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs.

Fusion gene

Hchr

Hbp

Tchr

Tbp

Start in +/-13AA

End in +/-13AA

FusionNeoAntigen peptide

FusionNEoAntigen RNA sequence

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Information of the samples that have these potential fusion neoantigens of PRCC-BTF3L4

These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens.

Cancer type	Fusion gene	Hchr	Hbp	Henst	Tchr	Tbp	Tenst	Sample
BRCA	PRCC-BTF3L4	chr1	156738031	ENST00000271526	chr1	52549011	ENST00000313334	TCGA-E9-A1R3

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Potential target of CAR-T therapy development for PRCC-BTF3L4

Predicted 3D structure. We used RoseTTAFold.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features

* Minus value of BPloci means that the break point is located before the CDS.

- In-frame and retained 'Transmembrane'.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Subcellular localization prediction of the transmembrane domain retained fusion proteins
* We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image.

Hgene

Hchr

Hbp

Henst

Tgene

Tchr

Tbp

Tenst

DeepLoc result

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Related Drugs to PRCC-BTF3L4

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

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Related Diseases to PRCC-BTF3L4

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner	Gene	Disease ID	Disease name	# pubmeds	Source
Hgene	PRCC	C4518356	MiT family translocation renal cell carcinoma	3	ORPHANET

	Fusion Gene and Fusion Protein Summary
	Fusion Amino Acid Sequences (multiple BPs and multiple gene isoforms)
	Fusion Protein Breakpoint Sequences - (for the Screening of the FusionNeoAntigens)
	Potential FusionNeoAntigens in HLA I - (netMHCpan v4.1 + deepHLApan v1.1)
	Potential FusionNeoAntigens in HLA II - (netMHCIIpan v4.1)
	Fusion Breakpoint 14 AA Peptide Structure - (RoseTTAFold)
	Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D - (Glide)
	Vaccine Design for the FusionNeoAntigens (RNA/protein sequences)
	Potential target of CAR-T therapy development
	Information on the samples that have these potential fusion neoantigens
	Fusion Protein Targeting Drugs - (Manual Curation)
	Fusion Protein Related diseases - (Manual Curation)