FusionNeoAntigen Logo

Home

Download

Statistics

Examples

Help

Contact

Terms of Use
Center for Computational Systems Medicine
leaf

Fusion Gene and Fusion Protein Summary

leaf

Fusion Amino Acid Sequences (multiple BPs and multiple gene isoforms)

leaf

Fusion Protein Breakpoint Sequences - (for the Screening of the FusionNeoAntigens)

leaf

Potential FusionNeoAntigens in HLA I - (netMHCpan v4.1 + deepHLApan v1.1)

leaf

Potential FusionNeoAntigens in HLA II - (netMHCIIpan v4.1)

leaf

Fusion Breakpoint 14 AA Peptide Structure - (RoseTTAFold)

leaf

Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D - (Glide)

leaf

Vaccine Design for the FusionNeoAntigens (RNA/protein sequences)

leaf

Potential target of CAR-T therapy development

leaf

Information on the samples that have these potential fusion neoantigens

leaf

Fusion Protein Targeting Drugs - (Manual Curation)

leaf

Fusion Protein Related diseases - (Manual Curation)

Fusion Protein:ROCK2-PPP2CA

Fusion Gene and Fusion Protein Summary

check button Fusion gene summary
Fusion partner gene informationFusion gene name: ROCK2-PPP2CA
FusionPDB ID: 75337
FusionGDB2.0 ID: 75337
HgeneTgene
Gene symbol

ROCK2

PPP2CA

Gene ID

9475

5515

Gene nameRho associated coiled-coil containing protein kinase 2protein phosphatase 2 catalytic subunit alpha
SynonymsROCK-IINEDLBA|PP2Ac|PP2CA|PP2Calpha|RP-C
Cytomap

2p25.1

5q31.1

Type of geneprotein-codingprotein-coding
Descriptionrho-associated protein kinase 2p164 ROCK-2rho-associated, coiled-coil-containing protein kinase IIserine/threonine-protein phosphatase 2A catalytic subunit alpha isoformPP2A-alphaprotein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoformprotein phosphatase 2, catalytic subunit, alpha isozymereplication protein Cserine/threonine protein
Modification date2020032920200315
UniProtAcc..
Ensembl transtripts involved in fusion geneENST idsENST00000315872, ENST00000401753, 
ENST00000462366, 		ENST00000231504, 
ENST00000481195, 
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)* DoF score16 X 11 X 9=15848 X 8 X 5=320
# samples 1711
** MAII scorelog2(17/1584*10)=-3.21996568394191
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(11/320*10)=-1.5405683813627
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Fusion gene context

PubMed: ROCK2 [Title/Abstract] AND PPP2CA [Title/Abstract] AND fusion [Title/Abstract]

Fusion neoantigen context

PubMed: ROCK2 [Title/Abstract] AND PPP2CA [Title/Abstract] AND neoantigen [Title/Abstract]

Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)ROCK2(11484122)-PPP2CA(133541822), # samples:2
Anticipated loss of major functional domain due to fusion event.ROCK2-PPP2CA seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
ROCK2-PPP2CA seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgeneROCK2

GO:0006468

protein phosphorylation

18559669

TgenePPP2CA

GO:0035970

peptidyl-threonine dephosphorylation

30611118



check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr2:11484122/chr5:133541822)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonRetention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here.

check buttonFusion gene breakpoints across ROCK2 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check buttonFusion gene breakpoints across PPP2CA (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure


Top

Fusion Amino Acid Sequences


check buttonFusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000315872ROCK2chr211484122-ENST00000481195PPP2CAchr5133541822-48565904491417322
ENST00000315872ROCK2chr211484121-ENST00000481195PPP2CAchr5133541822-48565904491417322

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000315872ENST00000481195ROCK2chr211484122-PPP2CAchr5133541822-0.0001517640.99984825
ENST00000315872ENST00000481195ROCK2chr211484121-PPP2CAchr5133541822-0.0001517640.99984825

check button Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones.

Get the fusion protein sequences from here.

Fusion protein sequence information is available in the fasta format.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

Top

Fusion Protein Breakpoint Sequences for ROCK2-PPP2CA

check button +/-13 AA sequence from the breakpoints of the fusion protein sequences.
HgeneHchrHbpTgeneTchrTbpLength(fusion protein)BP in fusion proteinPeptide
ROCK2chr211484121PPP2CAchr513354182259040RKLEALIRDPRSPINVESLLAKEILT
ROCK2chr211484122PPP2CAchr513354182259040RKLEALIRDPRSPINVESLLAKEILT

Top

Potential FusionNeoAntigen Information of ROCK2-PPP2CA in HLA I

check button Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.
ROCK2-PPP2CA_11484121_133541822.msa

check button Potential FusionNeoAntigen Information
* We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5)
Fusion geneHchrHbpTgeneTchrTbpHLA IFusionNeoAntigen peptideBinding scoreImmunogenic scoreNeoantigen start (at BP 13)Neoantigen end (at BP 13)
ROCK2-PPP2CAchr211484121chr5133541822590HLA-B35:03SPINVESL0.95510.86241119
ROCK2-PPP2CAchr211484121chr5133541822590HLA-B35:04SPINVESL0.86080.7661119
ROCK2-PPP2CAchr211484121chr5133541822590HLA-B35:02SPINVESL0.86080.7661119
ROCK2-PPP2CAchr211484121chr5133541822590HLA-B35:03SPINVESLL0.98530.83991120
ROCK2-PPP2CAchr211484121chr5133541822590HLA-B35:01SPINVESLL0.98290.76721120
ROCK2-PPP2CAchr211484121chr5133541822590HLA-B35:08SPINVESLL0.96960.65571120
ROCK2-PPP2CAchr211484121chr5133541822590HLA-B35:02SPINVESLL0.90630.74441120
ROCK2-PPP2CAchr211484121chr5133541822590HLA-B35:04SPINVESLL0.90630.74441120
ROCK2-PPP2CAchr211484121chr5133541822590HLA-B07:12SPINVESL0.99050.50251119
ROCK2-PPP2CAchr211484121chr5133541822590HLA-B35:12SPINVESL0.86080.7661119
ROCK2-PPP2CAchr211484121chr5133541822590HLA-B39:10SPINVESL0.83740.90281119
ROCK2-PPP2CAchr211484121chr5133541822590HLA-C01:17RSPINVESL0.96710.92861019
ROCK2-PPP2CAchr211484121chr5133541822590HLA-B35:12SPINVESLL0.90630.74441120
ROCK2-PPP2CAchr211484121chr5133541822590HLA-B39:10SPINVESLL0.7770.88481120
ROCK2-PPP2CAchr211484121chr5133541822590HLA-C01:30RSPINVESL0.71940.94431019
ROCK2-PPP2CAchr211484121chr5133541822590HLA-B07:12DPRSPINVESL0.99770.5494819
ROCK2-PPP2CAchr211484121chr5133541822590HLA-B39:10DPRSPINVESL0.88440.83819
ROCK2-PPP2CAchr211484121chr5133541822590HLA-C07:04IRDPRSPI0.98790.853614
ROCK2-PPP2CAchr211484121chr5133541822590HLA-B35:13SPINVESL0.94660.86761119
ROCK2-PPP2CAchr211484121chr5133541822590HLA-B35:17SPINVESL0.89690.57291119
ROCK2-PPP2CAchr211484121chr5133541822590HLA-B35:30SPINVESL0.89690.57291119
ROCK2-PPP2CAchr211484121chr5133541822590HLA-B35:09SPINVESL0.86080.7661119
ROCK2-PPP2CAchr211484121chr5133541822590HLA-B67:01SPINVESL0.82320.67091119
ROCK2-PPP2CAchr211484121chr5133541822590HLA-B35:23SPINVESLL0.98510.68831120
ROCK2-PPP2CAchr211484121chr5133541822590HLA-B35:77SPINVESLL0.98290.76721120
ROCK2-PPP2CAchr211484121chr5133541822590HLA-B35:13SPINVESLL0.97910.84611120
ROCK2-PPP2CAchr211484121chr5133541822590HLA-C01:02RSPINVESL0.96630.92881019
ROCK2-PPP2CAchr211484121chr5133541822590HLA-C01:03RSPINVESL0.96180.91521019
ROCK2-PPP2CAchr211484121chr5133541822590HLA-B35:09SPINVESLL0.90630.74441120
ROCK2-PPP2CAchr211484121chr5133541822590HLA-B35:17SPINVESLL0.89120.5571120
ROCK2-PPP2CAchr211484121chr5133541822590HLA-B35:30SPINVESLL0.89120.5571120
ROCK2-PPP2CAchr211484121chr5133541822590HLA-B35:11SPINVESLL0.84280.81411120
ROCK2-PPP2CAchr211484121chr5133541822590HLA-B35:24SPINVESLL0.83810.7421120
ROCK2-PPP2CAchr211484121chr5133541822590HLA-B67:01SPINVESLL0.8020.65421120
ROCK2-PPP2CAchr211484121chr5133541822590HLA-B07:13RSPINVESL0.47060.84331019
ROCK2-PPP2CAchr211484121chr5133541822590HLA-B35:43SPINVESLL0.0320.77811120
ROCK2-PPP2CAchr211484121chr5133541822590HLA-C01:03RSPINVESLL0.97990.92291020
ROCK2-PPP2CAchr211484121chr5133541822590HLA-C01:02RSPINVESLL0.95950.93541020
ROCK2-PPP2CAchr211484121chr5133541822590HLA-C07:04IRDPRSPINV0.91450.936616

Top

Potential FusionNeoAntigen Information of ROCK2-PPP2CA in HLA II

check button Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.
ROCK2-PPP2CA_11484121_133541822.msa

check button Potential FusionNeoAntigen Information
* We used NetMHCIIpan v4.1 (%rank<0.5).
Fusion geneHchrHbpTgeneTchrTbpHLA IIFusionNeoAntigen peptideNeoantigen start (at BP 13)Neoantigen end (at BP 13)
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0301LEALIRDPRSPINVE217
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0301KLEALIRDPRSPINV116
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0301EALIRDPRSPINVES318
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0301RKLEALIRDPRSPIN015
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0303LEALIRDPRSPINVE217
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0303KLEALIRDPRSPINV116
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0303EALIRDPRSPINVES318
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0303RKLEALIRDPRSPIN015
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0305LEALIRDPRSPINVE217
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0305KLEALIRDPRSPINV116
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0305EALIRDPRSPINVES318
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0305RKLEALIRDPRSPIN015
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0307LEALIRDPRSPINVE217
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0307KLEALIRDPRSPINV116
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0307EALIRDPRSPINVES318
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0307RKLEALIRDPRSPIN015
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0310LEALIRDPRSPINVE217
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0310KLEALIRDPRSPINV116
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0310RKLEALIRDPRSPIN015
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0310EALIRDPRSPINVES318
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0313LEALIRDPRSPINVE217
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0313KLEALIRDPRSPINV116
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0313EALIRDPRSPINVES318
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0313RKLEALIRDPRSPIN015
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0315LEALIRDPRSPINVE217
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0315KLEALIRDPRSPINV116
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0315EALIRDPRSPINVES318
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0315RKLEALIRDPRSPIN015
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0318LEALIRDPRSPINVE217
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0318KLEALIRDPRSPINV116
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0318EALIRDPRSPINVES318
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0318RKLEALIRDPRSPIN015
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0320LEALIRDPRSPINVE217
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0320KLEALIRDPRSPINV116
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0320EALIRDPRSPINVES318
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0320RKLEALIRDPRSPIN015
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0322LEALIRDPRSPINVE217
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0322KLEALIRDPRSPINV116
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0322EALIRDPRSPINVES318
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0322RKLEALIRDPRSPIN015
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0324LEALIRDPRSPINVE217
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0324KLEALIRDPRSPINV116
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0324EALIRDPRSPINVES318
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0324RKLEALIRDPRSPIN015
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0326LEALIRDPRSPINVE217
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0326KLEALIRDPRSPINV116
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0326EALIRDPRSPINVES318
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0326RKLEALIRDPRSPIN015
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0328LEALIRDPRSPINVE217
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0328KLEALIRDPRSPINV116
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0328EALIRDPRSPINVES318
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0328RKLEALIRDPRSPIN015
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0330LEALIRDPRSPINVE217
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0330KLEALIRDPRSPINV116
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0330EALIRDPRSPINVES318
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0330RKLEALIRDPRSPIN015
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0332LEALIRDPRSPINVE217
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0332KLEALIRDPRSPINV116
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0332EALIRDPRSPINVES318
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0332RKLEALIRDPRSPIN015
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0334LEALIRDPRSPINVE217
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0334KLEALIRDPRSPINV116
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0334EALIRDPRSPINVES318
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0334RKLEALIRDPRSPIN015
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0336LEALIRDPRSPINVE217
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0336KLEALIRDPRSPINV116
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0336EALIRDPRSPINVES318
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0336RKLEALIRDPRSPIN015
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0338LEALIRDPRSPINVE217
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0338KLEALIRDPRSPINV116
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0338EALIRDPRSPINVES318
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0338RKLEALIRDPRSPIN015
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0340LEALIRDPRSPINVE217
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0340KLEALIRDPRSPINV116
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0340EALIRDPRSPINVES318
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0340RKLEALIRDPRSPIN015
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0342LEALIRDPRSPINVE217
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0342KLEALIRDPRSPINV116
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0344LEALIRDPRSPINVE217
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0344KLEALIRDPRSPINV116
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0344EALIRDPRSPINVES318
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0344RKLEALIRDPRSPIN015
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0346LEALIRDPRSPINVE217
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0346KLEALIRDPRSPINV116
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0346EALIRDPRSPINVES318
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0346RKLEALIRDPRSPIN015
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0348LEALIRDPRSPINVE217
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0348KLEALIRDPRSPINV116
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0348EALIRDPRSPINVES318
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0348RKLEALIRDPRSPIN015
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0350LEALIRDPRSPINVE217
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0350KLEALIRDPRSPINV116
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0350EALIRDPRSPINVES318
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0350RKLEALIRDPRSPIN015
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0352LEALIRDPRSPINVE217
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0352KLEALIRDPRSPINV116
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0352EALIRDPRSPINVES318
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0352RKLEALIRDPRSPIN015
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0354LEALIRDPRSPINVE217
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0354KLEALIRDPRSPINV116
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0354EALIRDPRSPINVES318
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-0354RKLEALIRDPRSPIN015
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-1107LEALIRDPRSPINVE217
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-1107KLEALIRDPRSPINV116
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-1107EALIRDPRSPINVES318
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-1107RKLEALIRDPRSPIN015
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-1179LEALIRDPRSPINVE217
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-1333LEALIRDPRSPINVE217
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-1394LEALIRDPRSPINVE217
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-1394KLEALIRDPRSPINV116
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-1419LEALIRDPRSPINVE217
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-1419KLEALIRDPRSPINV116
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-1421LEALIRDPRSPINVE217
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-1421KLEALIRDPRSPINV116
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-1438LEALIRDPRSPINVE217
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-1438KLEALIRDPRSPINV116
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-1449LEALIRDPRSPINVE217
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-1450LEALIRDPRSPINVE217
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-1476LEALIRDPRSPINVE217
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-1476KLEALIRDPRSPINV116
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-1476RKLEALIRDPRSPIN015
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-1476EALIRDPRSPINVES318
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-1479LEALIRDPRSPINVE217
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-1479KLEALIRDPRSPINV116
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-1479RKLEALIRDPRSPIN015
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-1479EALIRDPRSPINVES318
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-1482LEALIRDPRSPINVE217
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-1482KLEALIRDPRSPINV116
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-1482RKLEALIRDPRSPIN015
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-1482EALIRDPRSPINVES318
ROCK2-PPP2CAchr211484121chr5133541822590DRB1-1495LEALIRDPRSPINVE217
ROCK2-PPP2CAchr211484121chr5133541822590DRB3-0114LEALIRDPRSPINVE217
ROCK2-PPP2CAchr211484121chr5133541822590DRB3-0114KLEALIRDPRSPINV116
ROCK2-PPP2CAchr211484121chr5133541822590DRB3-0114EALIRDPRSPINVES318

Top

Fusion breakpoint peptide structures of ROCK2-PPP2CA

check button3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens
* The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA.
File nameBPseqHgeneTgeneHchrHbpTchrTbpAAlen
3934IRDPRSPINVESLLROCK2PPP2CAchr211484121chr5133541822590

Top

Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of ROCK2-PPP2CA

check buttonVirtual screening between 25 HLAs (from PDB) and FusionNeoAntigens
* We used Glide to predict the interaction between HLAs and neoantigens.
HLA allelePDB IDFile nameBPseqDocking scoreGlide score
HLA-B14:023BVN3934IRDPRSPINVESLL-7.15543-7.26883
HLA-B14:023BVN3934IRDPRSPINVESLL-4.77435-5.80965
HLA-B52:013W393934IRDPRSPINVESLL-6.80875-6.92215
HLA-B52:013W393934IRDPRSPINVESLL-4.20386-5.23916
HLA-A11:014UQ23934IRDPRSPINVESLL-7.5194-8.5547
HLA-A11:014UQ23934IRDPRSPINVESLL-6.9601-7.0735
HLA-A24:025HGA3934IRDPRSPINVESLL-7.52403-7.63743
HLA-A24:025HGA3934IRDPRSPINVESLL-5.82433-6.85963
HLA-B27:056PYJ3934IRDPRSPINVESLL-3.28285-4.31815
HLA-B44:053DX83934IRDPRSPINVESLL-5.91172-6.94702
HLA-B44:053DX83934IRDPRSPINVESLL-4.24346-4.35686

Top

Vaccine Design for the FusionNeoAntigens of ROCK2-PPP2CA

check button mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is.
Fusion geneHchrHbpTchrTbpStart in +/-13AAEnd in +/-13AAFusionNeoAntigen peptide sequenceFusionNeoAntigen RNA sequence
ROCK2-PPP2CAchr211484121chr51335418221019RSPINVESLAGCTTGCTGGCTAAAGAAATCCTGACA
ROCK2-PPP2CAchr211484121chr51335418221020RSPINVESLLAGCTTGCTGGCTAAAGAAATCCTGACAAAA
ROCK2-PPP2CAchr211484121chr51335418221119SPINVESLTTGCTGGCTAAAGAAATCCTGACA
ROCK2-PPP2CAchr211484121chr51335418221120SPINVESLLTTGCTGGCTAAAGAAATCCTGACAAAA
ROCK2-PPP2CAchr211484121chr5133541822614IRDPRSPIATCAACGTGGAGAGCTTGCTGGCT
ROCK2-PPP2CAchr211484121chr5133541822616IRDPRSPINVATCAACGTGGAGAGCTTGCTGGCTAAAGAA
ROCK2-PPP2CAchr211484121chr5133541822819DPRSPINVESLGTGGAGAGCTTGCTGGCTAAAGAAATCCTGACA

check button mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs.
Fusion geneHchrHbpTchrTbpStart in +/-13AAEnd in +/-13AAFusionNeoAntigen peptideFusionNEoAntigen RNA sequence
ROCK2-PPP2CAchr211484121chr5133541822015RKLEALIRDPRSPINCGAGACCCTCGCTCCCCCATCAACGTGGAGAGCTTGCTGGCTAAA
ROCK2-PPP2CAchr211484121chr5133541822116KLEALIRDPRSPINVGACCCTCGCTCCCCCATCAACGTGGAGAGCTTGCTGGCTAAAGAA
ROCK2-PPP2CAchr211484121chr5133541822217LEALIRDPRSPINVECCTCGCTCCCCCATCAACGTGGAGAGCTTGCTGGCTAAAGAAATC
ROCK2-PPP2CAchr211484121chr5133541822318EALIRDPRSPINVESCGCTCCCCCATCAACGTGGAGAGCTTGCTGGCTAAAGAAATCCTG

Top

Information of the samples that have these potential fusion neoantigens of ROCK2-PPP2CA

check button These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens.
Cancer typeFusion geneHchrHbpHenstTchrTbpTenstSample
BRCAROCK2-PPP2CAchr211484121ENST00000315872chr5133541822ENST00000481195TCGA-BH-A0HL

Top

Potential target of CAR-T therapy development for ROCK2-PPP2CA

check button Predicted 3D structure. We used RoseTTAFold.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features


* Minus value of BPloci means that the break point is located before the CDS.
- In-frame and retained 'Transmembrane'.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

check button Subcellular localization prediction of the transmembrane domain retained fusion proteins
* We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image.
HgeneHchrHbpHenstTgeneTchrTbpTenstDeepLoc result

Top

Related Drugs to ROCK2-PPP2CA

check button Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDrugSourcePMID

Top

Related Diseases to ROCK2-PPP2CA

check button Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDiseaseSourcePMID

check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource