Home

Download

Statistics

Examples

Help

Contact

Fusion Protein:SH3GL1-MUM1

Fusion Gene and Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: SH3GL1-MUM1
	FusionPDB ID: 81455
	FusionGDB2.0 ID: 81455
		Hgene	Tgene
	Gene symbol	SH3GL1	MUM1
	Gene ID	6455	84939
	Gene name	SH3 domain containing GRB2 like 1, endophilin A2	PWWP domain containing 3A, DNA repair factor
	Synonyms	CNSA1\|EEN\|SH3D2B\|SH3P8	EXPAND1\|HSPC211\|MUM-1\|MUM1
	Cytomap	19p13.3	19p13.3
	Type of gene	protein-coding	protein-coding
	Description	endophilin-A2EEN fusion partner of MLLSH3 domain protein 2BSH3 domain-containing GRB2-like protein 1SH3-containing Grb-2-like 1 proteinSH3-domain GRB2-like 1endophilin-2extra 11-19 leukemia fusionextra eleven-nineteen leukemia fusion gene protein	PWWP domain-containing DNA repair factor 3APWWP domain-containing protein MUM1melanoma associated antigen (mutated) 1melanoma ubiquitous mutated proteinmutated melanoma-associated antigen 1protein expandere
	Modification date	20200313	20200313
	UniProtAcc	Q99961 Main function of 5'-partner protein: FUNCTION: Implicated in endocytosis. May recruit other proteins to membranes with high curvature (By similarity). {ECO:0000250}.	.
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000269886, ENST00000417295, ENST00000598564,	ENST00000311401, ENST00000344663, ENST00000415183, ENST00000591806, ENST00000591453,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	18 X 9 X 12=1944	10 X 10 X 4=400
	# samples	22	10
	** MAII score	log2(22/1944*10)=-3.14345279008112 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(10/400*10)=-2 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Fusion gene context	PubMed: SH3GL1 [Title/Abstract] AND MUM1 [Title/Abstract] AND fusion [Title/Abstract]
Fusion neoantigen context	PubMed: SH3GL1 [Title/Abstract] AND MUM1 [Title/Abstract] AND neoantigen [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	SH3GL1(4400321)-MUM1(1357008), # samples:1 SH3GL1(4400321)-MUM1(1356323), # samples:1
Anticipated loss of major functional domain due to fusion event.	SH3GL1-MUM1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. SH3GL1-MUM1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. SH3GL1-MUM1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. SH3GL1-MUM1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner

Gene

GO ID

GO term

PubMed ID

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr19:4400321/chr19:1357008)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Retention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here.

Fusion gene breakpoints across SH3GL1 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across MUM1 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Top

Fusion Amino Acid Sequences

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000269886	SH3GL1	chr19	4400321	-	ENST00000344663	MUM1	chr19	1357008	+	4155	224	179	2299	706
ENST00000269886	SH3GL1	chr19	4400321	-	ENST00000591806	MUM1	chr19	1357008	+	4154	224	179	2299	706
ENST00000598564	SH3GL1	chr19	4400321	-	ENST00000344663	MUM1	chr19	1357008	+	4026	95	50	2170	706
ENST00000598564	SH3GL1	chr19	4400321	-	ENST00000591806	MUM1	chr19	1357008	+	4025	95	50	2170	706
ENST00000417295	SH3GL1	chr19	4400321	-	ENST00000344663	MUM1	chr19	1357008	+	4133	202	157	2277	706
ENST00000417295	SH3GL1	chr19	4400321	-	ENST00000591806	MUM1	chr19	1357008	+	4132	202	157	2277	706

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000269886	ENST00000344663	SH3GL1	chr19	4400321	-	MUM1	chr19	1357008	+	0.005085463	0.99491453
ENST00000269886	ENST00000591806	SH3GL1	chr19	4400321	-	MUM1	chr19	1357008	+	0.005066198	0.9949338
ENST00000598564	ENST00000344663	SH3GL1	chr19	4400321	-	MUM1	chr19	1357008	+	0.004836164	0.9951638
ENST00000598564	ENST00000591806	SH3GL1	chr19	4400321	-	MUM1	chr19	1357008	+	0.004819015	0.99518096
ENST00000417295	ENST00000344663	SH3GL1	chr19	4400321	-	MUM1	chr19	1357008	+	0.005186258	0.9948138
ENST00000417295	ENST00000591806	SH3GL1	chr19	4400321	-	MUM1	chr19	1357008	+	0.00516779	0.99483216

Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones.

Get the fusion protein sequences from here.

Fusion protein sequence information is available in the fasta format.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

Top

Fusion Protein Breakpoint Sequences for SH3GL1-MUM1

+/-13 AA sequence from the breakpoints of the fusion protein sequences.

Hgene	Hchr	Hbp	Tgene	Tchr	Tbp	Length(fusion protein)	BP in fusion protein	Peptide
SH3GL1	chr19	4400321	MUM1	chr19	1357008	202	15	VAGLKKQFYKASQVLARTATSTKNKR
SH3GL1	chr19	4400321	MUM1	chr19	1357008	224	15	VAGLKKQFYKASQVLARTATSTKNKR
SH3GL1	chr19	4400321	MUM1	chr19	1357008	95	15	VAGLKKQFYKASQVLARTATSTKNKR

Top

Potential FusionNeoAntigen Information of SH3GL1-MUM1 in HLA I

Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.

SH3GL1-MUM1_4400321_1357008.msa

Potential FusionNeoAntigen Information
* We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5)

Fusion gene	Hchr	Hbp	Tgene	Tchr	Tbp	HLA I	FusionNeoAntigen peptide	Binding score	Immunogenic score	Neoantigen start (at BP 13)	Neoantigen end (at BP 13)
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-B48:01	KQFYKASQV	0.9785	0.607	5	14
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-A30:08	KQFYKASQV	0.9665	0.8878	5	14
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-A02:21	KQFYKASQV	0.96	0.7372	5	14
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-A02:22	KQFYKASQV	0.9537	0.6128	5	14
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-A02:04	KQFYKASQV	0.9405	0.7947	5	14
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-A02:13	KQFYKASQV	0.9286	0.735	5	14
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-B13:02	KQFYKASQV	0.8673	0.9142	5	14
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-A02:20	KQFYKASQV	0.7891	0.6359	5	14
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-B13:01	KQFYKASQV	0.705	0.9922	5	14
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-B15:37	QFYKASQVL	0.4833	0.6047	6	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-B15:10	QFYKASQVL	0.4149	0.6228	6	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-B39:13	KQFYKASQV	0.4047	0.9812	5	14
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-B52:01	KQFYKASQV	0.0344	0.9771	5	14
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-B15:01	KQFYKASQVL	0.9983	0.9345	5	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-B48:01	KQFYKASQVL	0.9853	0.5373	5	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-A31:02	FYKASQVLAR	0.9677	0.8586	7	17
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-B15:03	KQFYKASQVL	0.9393	0.8531	5	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-B39:13	KQFYKASQVL	0.8371	0.9595	5	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-B13:02	KQFYKASQVL	0.7644	0.722	5	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-B13:01	KQFYKASQVL	0.6164	0.9876	5	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-B13:02	KKQFYKASQV	0.5127	0.7928	4	14
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-A11:03	QVLARTATSTK	0.9984	0.5461	12	23
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-A31:02	QFYKASQVLAR	0.9507	0.8354	6	17
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-C12:16	FYKASQVL	0.9972	0.9445	7	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-C07:05	FYKASQVL	0.9874	0.9397	7	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-C07:27	FYKASQVL	0.9809	0.9273	7	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-C07:29	FYKASQVL	0.9797	0.9074	7	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-C07:10	FYKASQVL	0.9776	0.9285	7	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-C07:13	FYKASQVL	0.9774	0.8896	7	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-C07:67	FYKASQVL	0.9766	0.911	7	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-C07:80	FYKASQVL	0.9766	0.911	7	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-C06:03	KQFYKASQV	0.9834	0.9952	5	14
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-A02:05	KQFYKASQV	0.9715	0.5752	5	14
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-B15:04	KQFYKASQV	0.8296	0.9691	5	14
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-B48:03	KQFYKASQV	0.6618	0.5001	5	14
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-B15:21	QFYKASQVL	0.5429	0.9368	6	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-C04:14	QFYKASQVL	0.5035	0.9599	6	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-C07:05	QFYKASQVL	0.4869	0.9787	6	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-C07:10	QFYKASQVL	0.4834	0.9776	6	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-C07:29	QFYKASQVL	0.4661	0.9552	6	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-B14:03	QFYKASQVL	0.4598	0.8221	6	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-C07:13	QFYKASQVL	0.4598	0.9489	6	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-C07:67	QFYKASQVL	0.4593	0.9692	6	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-C07:80	QFYKASQVL	0.4593	0.9692	6	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-C07:19	QFYKASQVL	0.4411	0.8511	6	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-C07:46	QFYKASQVL	0.4242	0.9446	6	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-C07:27	QFYKASQVL	0.3303	0.9692	6	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-C01:30	QFYKASQVL	0.1259	0.9573	6	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-B15:04	KQFYKASQVL	0.9806	0.9556	5	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-B15:07	KQFYKASQVL	0.9769	0.8178	5	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-C03:67	FYKASQVL	0.9922	0.9827	7	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-C04:04	FYKASQVL	0.9846	0.8603	7	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-C07:04	FYKASQVL	0.9824	0.9254	7	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-C07:02	FYKASQVL	0.9766	0.911	7	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-C07:17	FYKASQVL	0.9755	0.9386	7	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-C06:06	FYKASQVL	0.9753	0.9703	7	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-C14:02	FYKASQVL	0.9105	0.9151	7	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-C14:03	FYKASQVL	0.9105	0.9151	7	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-A02:03	KQFYKASQV	0.9823	0.7845	5	14
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-C06:08	KQFYKASQV	0.962	0.9915	5	14
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-A02:14	KQFYKASQV	0.9608	0.7142	5	14
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-A02:06	KQFYKASQV	0.96	0.7372	5	14
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-C06:17	KQFYKASQV	0.9285	0.9943	5	14
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-C06:02	KQFYKASQV	0.9285	0.9943	5	14
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-B15:73	KQFYKASQV	0.8599	0.9778	5	14
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-B15:68	KQFYKASQV	0.8175	0.8186	5	14
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-B15:30	KQFYKASQV	0.768	0.9513	5	14
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-C03:67	QFYKASQVL	0.7581	0.9839	6	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-B15:54	KQFYKASQV	0.7393	0.935	5	14
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-B40:12	KQFYKASQV	0.6618	0.5001	5	14
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-C07:04	QFYKASQVL	0.6198	0.9672	6	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-B40:21	KQFYKASQV	0.5641	0.5655	5	14
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-B39:02	KQFYKASQV	0.5037	0.9813	5	14
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-C07:02	QFYKASQVL	0.4593	0.9692	6	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-C07:17	QFYKASQVL	0.4557	0.9691	6	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-C04:04	QFYKASQVL	0.4182	0.9209	6	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-C14:03	QFYKASQVL	0.3294	0.9742	6	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-C14:02	QFYKASQVL	0.3294	0.9742	6	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-C06:06	QFYKASQVL	0.319	0.9892	6	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-B15:09	QFYKASQVL	0.1204	0.9201	6	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-B15:33	KQFYKASQVL	0.9983	0.9345	5	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-B15:125	KQFYKASQVL	0.9983	0.9345	5	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-B15:34	KQFYKASQVL	0.9983	0.9345	5	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-B15:50	KQFYKASQVL	0.9973	0.9487	5	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-B15:53	KQFYKASQVL	0.9947	0.9237	5	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-B15:68	KQFYKASQVL	0.9943	0.7563	5	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-B15:54	KQFYKASQVL	0.9904	0.9163	5	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-B15:73	KQFYKASQVL	0.9874	0.975	5	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-B15:35	KQFYKASQVL	0.9741	0.9283	5	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-B15:30	KQFYKASQVL	0.973	0.9462	5	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-B39:02	KQFYKASQVL	0.9349	0.9571	5	15
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	HLA-A30:01	QVLARTATSTK	0.9894	0.9305	12	23

Top

Potential FusionNeoAntigen Information of SH3GL1-MUM1 in HLA II

SH3GL1-MUM1_4400321_1357008.msa

Potential FusionNeoAntigen Information
* We used NetMHCIIpan v4.1 (%rank<0.5).

Fusion gene	Hchr	Hbp	Tgene	Tchr	Tbp	HLA II	FusionNeoAntigen peptide	Neoantigen start (at BP 13)	Neoantigen end (at BP 13)
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-0340	KQFYKASQVLARTAT	5	20
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-0434	KKQFYKASQVLARTA	4	19
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-0813	KKQFYKASQVLARTA	4	19
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-0815	KKQFYKASQVLARTA	4	19
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-0830	KKQFYKASQVLARTA	4	19
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-0901	KKQFYKASQVLARTA	4	19
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-0901	LKKQFYKASQVLART	3	18
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-0901	GLKKQFYKASQVLAR	2	17
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-0902	KKQFYKASQVLARTA	4	19
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-0902	LKKQFYKASQVLART	3	18
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-0902	KQFYKASQVLARTAT	5	20
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-0902	GLKKQFYKASQVLAR	2	17
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-0903	KKQFYKASQVLARTA	4	19
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-0903	LKKQFYKASQVLART	3	18
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-0903	GLKKQFYKASQVLAR	2	17
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-0903	KQFYKASQVLARTAT	5	20
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-0904	KKQFYKASQVLARTA	4	19
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-0904	LKKQFYKASQVLART	3	18
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-0904	GLKKQFYKASQVLAR	2	17
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-0905	KKQFYKASQVLARTA	4	19
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-0905	LKKQFYKASQVLART	3	18
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-0905	GLKKQFYKASQVLAR	2	17
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-0905	KQFYKASQVLARTAT	5	20
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-0907	KKQFYKASQVLARTA	4	19
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-0907	LKKQFYKASQVLART	3	18
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-0907	GLKKQFYKASQVLAR	2	17
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-0908	KKQFYKASQVLARTA	4	19
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-0908	LKKQFYKASQVLART	3	18
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-0908	KQFYKASQVLARTAT	5	20
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-0908	GLKKQFYKASQVLAR	2	17
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-0909	KKQFYKASQVLARTA	4	19
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-0909	LKKQFYKASQVLART	3	18
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-0909	GLKKQFYKASQVLAR	2	17
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-1130	KKQFYKASQVLARTA	4	19
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-1130	LKKQFYKASQVLART	3	18
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-1145	KKQFYKASQVLARTA	4	19
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-1172	KKQFYKASQVLARTA	4	19
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-1367	KKQFYKASQVLARTA	4	19
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-1385	KKQFYKASQVLARTA	4	19
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-1385	KQFYKASQVLARTAT	5	20
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-1386	KKQFYKASQVLARTA	4	19
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-1386	KQFYKASQVLARTAT	5	20
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-1402	KKQFYKASQVLARTA	4	19
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-1402	KQFYKASQVLARTAT	5	20
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-1402	LKKQFYKASQVLART	3	18
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-1403	KKQFYKASQVLARTA	4	19
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-1409	KKQFYKASQVLARTA	4	19
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-1414	KKQFYKASQVLARTA	4	19
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-1419	KKQFYKASQVLARTA	4	19
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-1419	KQFYKASQVLARTAT	5	20
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-1424	KKQFYKASQVLARTA	4	19
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-1424	KQFYKASQVLARTAT	5	20
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-1430	KKQFYKASQVLARTA	4	19
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-1436	KKQFYKASQVLARTA	4	19
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-1440	KKQFYKASQVLARTA	4	19
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-1441	KKQFYKASQVLARTA	4	19
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-1441	KQFYKASQVLARTAT	5	20
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-1441	LKKQFYKASQVLART	3	18
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-1442	KKQFYKASQVLARTA	4	19
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-1444	KKQFYKASQVLARTA	4	19
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-1446	KKQFYKASQVLARTA	4	19
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-1446	KQFYKASQVLARTAT	5	20
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-1446	LKKQFYKASQVLART	3	18
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-1447	KKQFYKASQVLARTA	4	19
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-1447	KQFYKASQVLARTAT	5	20
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-1447	LKKQFYKASQVLART	3	18
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-1451	KKQFYKASQVLARTA	4	19
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-1451	KQFYKASQVLARTAT	5	20
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-1451	LKKQFYKASQVLART	3	18
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-1467	KKQFYKASQVLARTA	4	19
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-1477	KKQFYKASQVLARTA	4	19
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-1489	KKQFYKASQVLARTA	4	19
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-1489	KQFYKASQVLARTAT	5	20
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-1489	LKKQFYKASQVLART	3	18
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-1494	KKQFYKASQVLARTA	4	19
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-1494	KQFYKASQVLARTAT	5	20
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-1494	LKKQFYKASQVLART	3	18
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-1498	KKQFYKASQVLARTA	4	19
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-1498	KQFYKASQVLARTAT	5	20
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB1-1498	LKKQFYKASQVLART	3	18
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB5-0101	KQFYKASQVLARTAT	5	20
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB5-0101	KKQFYKASQVLARTA	4	19
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB5-0102	KKQFYKASQVLARTA	4	19
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB5-0102	KQFYKASQVLARTAT	5	20
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB5-0103	KKQFYKASQVLARTA	4	19
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB5-0104	KKQFYKASQVLARTA	4	19
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB5-0104	KQFYKASQVLARTAT	5	20
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB5-0104	LKKQFYKASQVLART	3	18
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB5-0105	KQFYKASQVLARTAT	5	20
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB5-0105	KKQFYKASQVLARTA	4	19
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB5-0108N	KKQFYKASQVLARTA	4	19
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB5-0108N	KQFYKASQVLARTAT	5	20
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB5-0111	KQFYKASQVLARTAT	5	20
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB5-0111	KKQFYKASQVLARTA	4	19
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB5-0112	KKQFYKASQVLARTA	4	19
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB5-0112	KQFYKASQVLARTAT	5	20
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB5-0112	LKKQFYKASQVLART	3	18
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB5-0112	GLKKQFYKASQVLAR	2	17
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB5-0112	QFYKASQVLARTATS	6	21
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB5-0113	KQFYKASQVLARTAT	5	20
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB5-0113	KKQFYKASQVLARTA	4	19
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB5-0114	KQFYKASQVLARTAT	5	20
SH3GL1-MUM1	chr19	4400321	chr19	1357008	224	DRB5-0114	KKQFYKASQVLARTA	4	19

Top

Fusion breakpoint peptide structures of SH3GL1-MUM1

3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens
* The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA.

File name	BPseq	Hgene	Tgene	Hchr	Hbp	Tchr	Tbp	AAlen
7269	QFYKASQVLARTAT	SH3GL1	MUM1	chr19	4400321	chr19	1357008	224

Top

Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of SH3GL1-MUM1

Virtual screening between 25 HLAs (from PDB) and FusionNeoAntigens
* We used Glide to predict the interaction between HLAs and neoantigens.

HLA allele	PDB ID	File name	BPseq	Docking score	Glide score
HLA-B14:02	3BVN	7269	QFYKASQVLARTAT	-7.9962	-8.1096
HLA-B14:02	3BVN	7269	QFYKASQVLARTAT	-5.70842	-6.74372
HLA-B52:01	3W39	7269	QFYKASQVLARTAT	-6.83737	-6.95077
HLA-B52:01	3W39	7269	QFYKASQVLARTAT	-4.4836	-5.5189
HLA-A11:01	4UQ2	7269	QFYKASQVLARTAT	-10.0067	-10.1201
HLA-A11:01	4UQ2	7269	QFYKASQVLARTAT	-9.03915	-10.0745
HLA-A24:02	5HGA	7269	QFYKASQVLARTAT	-6.56204	-6.67544
HLA-A24:02	5HGA	7269	QFYKASQVLARTAT	-5.42271	-6.45801
HLA-B44:05	3DX8	7269	QFYKASQVLARTAT	-7.85648	-8.89178
HLA-B44:05	3DX8	7269	QFYKASQVLARTAT	-5.3978	-5.5112
HLA-A02:01	6TDR	7269	QFYKASQVLARTAT	-3.37154	-4.40684

Top

Vaccine Design for the FusionNeoAntigens of SH3GL1-MUM1

mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is.

Fusion gene	Hchr	Hbp	Tchr	Tbp	Start in +/-13AA	End in +/-13AA	FusionNeoAntigen peptide sequence	FusionNeoAntigen RNA sequence
SH3GL1-MUM1	chr19	4400321	chr19	1357008	12	23	QVLARTATSTK	CAGGTTTTGGCCCGAACCGCGACTTCAACAAAA
SH3GL1-MUM1	chr19	4400321	chr19	1357008	4	14	KKQFYKASQV	AAGAAGCAGTTCTACAAGGCGAGCCAGGTT
SH3GL1-MUM1	chr19	4400321	chr19	1357008	5	14	KQFYKASQV	AAGCAGTTCTACAAGGCGAGCCAGGTT
SH3GL1-MUM1	chr19	4400321	chr19	1357008	5	15	KQFYKASQVL	AAGCAGTTCTACAAGGCGAGCCAGGTTTTG
SH3GL1-MUM1	chr19	4400321	chr19	1357008	6	15	QFYKASQVL	CAGTTCTACAAGGCGAGCCAGGTTTTG
SH3GL1-MUM1	chr19	4400321	chr19	1357008	6	17	QFYKASQVLAR	CAGTTCTACAAGGCGAGCCAGGTTTTGGCCCGA
SH3GL1-MUM1	chr19	4400321	chr19	1357008	7	15	FYKASQVL	TTCTACAAGGCGAGCCAGGTTTTG
SH3GL1-MUM1	chr19	4400321	chr19	1357008	7	17	FYKASQVLAR	TTCTACAAGGCGAGCCAGGTTTTGGCCCGA

mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs.

Fusion gene	Hchr	Hbp	Tchr	Tbp	Start in +/-13AA	End in +/-13AA	FusionNeoAntigen peptide	FusionNEoAntigen RNA sequence
SH3GL1-MUM1	chr19	4400321	chr19	1357008	2	17	GLKKQFYKASQVLAR	GGGCTGAAGAAGCAGTTCTACAAGGCGAGCCAGGTTTTGGCCCGA
SH3GL1-MUM1	chr19	4400321	chr19	1357008	3	18	LKKQFYKASQVLART	CTGAAGAAGCAGTTCTACAAGGCGAGCCAGGTTTTGGCCCGAACC
SH3GL1-MUM1	chr19	4400321	chr19	1357008	4	19	KKQFYKASQVLARTA	AAGAAGCAGTTCTACAAGGCGAGCCAGGTTTTGGCCCGAACCGCG
SH3GL1-MUM1	chr19	4400321	chr19	1357008	5	20	KQFYKASQVLARTAT	AAGCAGTTCTACAAGGCGAGCCAGGTTTTGGCCCGAACCGCGACT
SH3GL1-MUM1	chr19	4400321	chr19	1357008	6	21	QFYKASQVLARTATS	CAGTTCTACAAGGCGAGCCAGGTTTTGGCCCGAACCGCGACTTCA

Top

Information of the samples that have these potential fusion neoantigens of SH3GL1-MUM1

These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens.

Cancer type	Fusion gene	Hchr	Hbp	Henst	Tchr	Tbp	Tenst	Sample
BRCA	SH3GL1-MUM1	chr19	4400321	ENST00000269886	chr19	1357008	ENST00000344663	TCGA-AN-A04A-01A

Top

Potential target of CAR-T therapy development for SH3GL1-MUM1

Predicted 3D structure. We used RoseTTAFold.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features

* Minus value of BPloci means that the break point is located before the CDS.

- In-frame and retained 'Transmembrane'.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Subcellular localization prediction of the transmembrane domain retained fusion proteins
* We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image.

Hgene

Hchr

Hbp

Henst

Tgene

Tchr

Tbp

Tenst

DeepLoc result

Top

Related Drugs to SH3GL1-MUM1

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

Top

Related Diseases to SH3GL1-MUM1

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source

	Fusion Gene and Fusion Protein Summary
	Fusion Amino Acid Sequences (multiple BPs and multiple gene isoforms)
	Fusion Protein Breakpoint Sequences - (for the Screening of the FusionNeoAntigens)
	Potential FusionNeoAntigens in HLA I - (netMHCpan v4.1 + deepHLApan v1.1)
	Potential FusionNeoAntigens in HLA II - (netMHCIIpan v4.1)
	Fusion Breakpoint 14 AA Peptide Structure - (RoseTTAFold)
	Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D - (Glide)
	Vaccine Design for the FusionNeoAntigens (RNA/protein sequences)
	Potential target of CAR-T therapy development
	Information on the samples that have these potential fusion neoantigens
	Fusion Protein Targeting Drugs - (Manual Curation)
	Fusion Protein Related diseases - (Manual Curation)