FusionNeoAntigen Logo

Home

Download

Statistics

Examples

Help

Contact

Terms of Use
Center for Computational Systems Medicine
leaf

Fusion Gene and Fusion Protein Summary

leaf

Fusion Amino Acid Sequences (multiple BPs and multiple gene isoforms)

leaf

Fusion Protein Breakpoint Sequences - (for the Screening of the FusionNeoAntigens)

leaf

Potential FusionNeoAntigens in HLA I - (netMHCpan v4.1 + deepHLApan v1.1)

leaf

Potential FusionNeoAntigens in HLA II - (netMHCIIpan v4.1)

leaf

Fusion Breakpoint 14 AA Peptide Structure - (RoseTTAFold)

leaf

Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D - (Glide)

leaf

Vaccine Design for the FusionNeoAntigens (RNA/protein sequences)

leaf

Potential target of CAR-T therapy development

leaf

Information on the samples that have these potential fusion neoantigens

leaf

Fusion Protein Targeting Drugs - (Manual Curation)

leaf

Fusion Protein Related diseases - (Manual Curation)

Fusion Protein:SLC25A3-EZH2

Fusion Gene and Fusion Protein Summary

check button Fusion gene summary
Fusion partner gene informationFusion gene name: SLC25A3-EZH2
FusionPDB ID: 82679
FusionGDB2.0 ID: 82679
HgeneTgene
Gene symbol

SLC25A3

EZH2

Gene ID

5250

2146

Gene namesolute carrier family 25 member 3enhancer of zeste 2 polycomb repressive complex 2 subunit
SynonymsOK/SW-cl.48|PHC|PTPENX-1|ENX1|EZH2b|KMT6|KMT6A|WVS|WVS2
Cytomap

12q23.1

7q36.1

Type of geneprotein-codingprotein-coding
Descriptionphosphate carrier protein, mitochondrialphosphate transport proteinsolute carrier family 25 (mitochondrial carrier; phosphate carrier), member 3histone-lysine N-methyltransferase EZH2enhancer of zeste homolog 2lysine N-methyltransferase 6
Modification date2020031320200329
UniProtAcc.

Q15910

Main function of 5'-partner protein: FUNCTION: Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Displays a preference for substrates with less methylation, loses activity when progressively more methyl groups are incorporated into H3K27, H3K27me0 > H3K27me1 > H3K27me2 (PubMed:22323599, PubMed:30923826). Compared to EZH1-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4 and the nuclear receptor RORA. Regulates the circadian clock via histone methylation at the promoter of the circadian genes. Essential for the CRY1/2-mediated repression of the transcriptional activation of PER1/2 by the CLOCK-ARNTL/BMAL1 heterodimer; involved in the di and trimethylation of 'Lys-27' of histone H3 on PER1/2 promoters which is necessary for the CRY1/2 proteins to inhibit transcription. {ECO:0000269|PubMed:14532106, ECO:0000269|PubMed:15225548, ECO:0000269|PubMed:15231737, ECO:0000269|PubMed:15385962, ECO:0000269|PubMed:16179254, ECO:0000269|PubMed:16357870, ECO:0000269|PubMed:16618801, ECO:0000269|PubMed:16717091, ECO:0000269|PubMed:16936726, ECO:0000269|PubMed:17210787, ECO:0000269|PubMed:17344414, ECO:0000269|PubMed:18285464, ECO:0000269|PubMed:19026781, ECO:0000269|PubMed:20935635, ECO:0000269|PubMed:22323599, ECO:0000269|PubMed:23063525, ECO:0000269|PubMed:24474760, ECO:0000269|PubMed:30923826}.
Ensembl transtripts involved in fusion geneENST idsENST00000188376, ENST00000228318, 
ENST00000401722, ENST00000547534, 
ENST00000548847, ENST00000549338, 
ENST00000551265, ENST00000551917, 
ENST00000552981, 		ENST00000536783, 
ENST00000320356, ENST00000350995, 
ENST00000460911, ENST00000476773, 
ENST00000478654, ENST00000483967, 
ENST00000541220, 
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)* DoF score13 X 12 X 4=62410 X 7 X 5=350
# samples 139
** MAII scorelog2(13/624*10)=-2.26303440583379
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(9/350*10)=-1.95935801550265
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Fusion gene context

PubMed: SLC25A3 [Title/Abstract] AND EZH2 [Title/Abstract] AND fusion [Title/Abstract]

Fusion neoantigen context

PubMed: SLC25A3 [Title/Abstract] AND EZH2 [Title/Abstract] AND neoantigen [Title/Abstract]

Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)SLC25A3(98987913)-EZH2(148511229), # samples:1
Anticipated loss of major functional domain due to fusion event.SLC25A3-EZH2 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
SLC25A3-EZH2 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
SLC25A3-EZH2 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
SLC25A3-EZH2 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
SLC25A3-EZH2 seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF.
SLC25A3-EZH2 seems lost the major protein functional domain in Hgene partner, which is a IUPHAR drug target due to the frame-shifted ORF.
SLC25A3-EZH2 seems lost the major protein functional domain in Tgene partner, which is a CGC due to the frame-shifted ORF.
SLC25A3-EZH2 seems lost the major protein functional domain in Tgene partner, which is a epigenetic factor due to the frame-shifted ORF.
SLC25A3-EZH2 seems lost the major protein functional domain in Tgene partner, which is a IUPHAR drug target due to the frame-shifted ORF.
SLC25A3-EZH2 seems lost the major protein functional domain in Tgene partner, which is a tumor suppressor due to the frame-shifted ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
TgeneEZH2

GO:0000122

negative regulation of transcription by RNA polymerase II

20154697

TgeneEZH2

GO:0010718

positive regulation of epithelial to mesenchymal transition

20154697

TgeneEZH2

GO:0043406

positive regulation of MAP kinase activity

20154697

TgeneEZH2

GO:0043547

positive regulation of GTPase activity

20154697

TgeneEZH2

GO:0045814

negative regulation of gene expression, epigenetic

20154697

TgeneEZH2

GO:0070734

histone H3-K27 methylation

24474760

TgeneEZH2

GO:0071902

positive regulation of protein serine/threonine kinase activity

20154697



check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr12:98987913/chr7:148511229)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonRetention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here.

check buttonFusion gene breakpoints across SLC25A3 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check buttonFusion gene breakpoints across EZH2 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure


Top

Fusion Amino Acid Sequences


check buttonFusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000401722SLC25A3chr1298987913+ENST00000478654EZH2chr7148511229-1153307150890246
ENST00000401722SLC25A3chr1298987913+ENST00000460911EZH2chr7148511229-1152307150890246
ENST00000401722SLC25A3chr1298987913+ENST00000350995EZH2chr7148511229-1152307150890246
ENST00000401722SLC25A3chr1298987913+ENST00000320356EZH2chr7148511229-1152307150890246
ENST00000401722SLC25A3chr1298987913+ENST00000541220EZH2chr7148511229-1152307150890246
ENST00000401722SLC25A3chr1298987913+ENST00000476773EZH2chr7148511229-1055307150890246
ENST00000401722SLC25A3chr1298987913+ENST00000483967EZH2chr7148511229-1012307150890246
ENST00000228318SLC25A3chr1298987913+ENST00000478654EZH2chr7148511229-112327793860255
ENST00000228318SLC25A3chr1298987913+ENST00000460911EZH2chr7148511229-112227793860255
ENST00000228318SLC25A3chr1298987913+ENST00000350995EZH2chr7148511229-112227793860255
ENST00000228318SLC25A3chr1298987913+ENST00000320356EZH2chr7148511229-112227793860255
ENST00000228318SLC25A3chr1298987913+ENST00000541220EZH2chr7148511229-112227793860255
ENST00000228318SLC25A3chr1298987913+ENST00000476773EZH2chr7148511229-102527793860255
ENST00000228318SLC25A3chr1298987913+ENST00000483967EZH2chr7148511229-98227793860255
ENST00000188376SLC25A3chr1298987913+ENST00000478654EZH2chr7148511229-13575111381094318
ENST00000188376SLC25A3chr1298987913+ENST00000460911EZH2chr7148511229-13565111381094318
ENST00000188376SLC25A3chr1298987913+ENST00000350995EZH2chr7148511229-13565111381094318
ENST00000188376SLC25A3chr1298987913+ENST00000320356EZH2chr7148511229-13565111381094318
ENST00000188376SLC25A3chr1298987913+ENST00000541220EZH2chr7148511229-13565111381094318
ENST00000188376SLC25A3chr1298987913+ENST00000476773EZH2chr7148511229-12595111381094318
ENST00000188376SLC25A3chr1298987913+ENST00000483967EZH2chr7148511229-12165111381094318
ENST00000551917SLC25A3chr1298987913+ENST00000478654EZH2chr7148511229-109424891831246
ENST00000551917SLC25A3chr1298987913+ENST00000460911EZH2chr7148511229-109324891831246
ENST00000551917SLC25A3chr1298987913+ENST00000350995EZH2chr7148511229-109324891831246
ENST00000551917SLC25A3chr1298987913+ENST00000320356EZH2chr7148511229-109324891831246
ENST00000551917SLC25A3chr1298987913+ENST00000541220EZH2chr7148511229-109324891831246
ENST00000551917SLC25A3chr1298987913+ENST00000476773EZH2chr7148511229-99624891831246
ENST00000551917SLC25A3chr1298987913+ENST00000483967EZH2chr7148511229-95324891831246
ENST00000552981SLC25A3chr1298987913+ENST00000478654EZH2chr7148511229-108824258825255
ENST00000552981SLC25A3chr1298987913+ENST00000460911EZH2chr7148511229-108724258825255
ENST00000552981SLC25A3chr1298987913+ENST00000350995EZH2chr7148511229-108724258825255
ENST00000552981SLC25A3chr1298987913+ENST00000320356EZH2chr7148511229-108724258825255
ENST00000552981SLC25A3chr1298987913+ENST00000541220EZH2chr7148511229-108724258825255
ENST00000552981SLC25A3chr1298987913+ENST00000476773EZH2chr7148511229-99024258825255
ENST00000552981SLC25A3chr1298987913+ENST00000483967EZH2chr7148511229-94724258825255
ENST00000547534SLC25A3chr1298987913+ENST00000478654EZH2chr7148511229-106221632799255
ENST00000547534SLC25A3chr1298987913+ENST00000460911EZH2chr7148511229-106121632799255
ENST00000547534SLC25A3chr1298987913+ENST00000350995EZH2chr7148511229-106121632799255
ENST00000547534SLC25A3chr1298987913+ENST00000320356EZH2chr7148511229-106121632799255
ENST00000547534SLC25A3chr1298987913+ENST00000541220EZH2chr7148511229-106121632799255
ENST00000547534SLC25A3chr1298987913+ENST00000476773EZH2chr7148511229-96421632799255
ENST00000547534SLC25A3chr1298987913+ENST00000483967EZH2chr7148511229-92121632799255
ENST00000549338SLC25A3chr1298987913+ENST00000478654EZH2chr7148511229-105621053793246
ENST00000549338SLC25A3chr1298987913+ENST00000460911EZH2chr7148511229-105521053793246
ENST00000549338SLC25A3chr1298987913+ENST00000350995EZH2chr7148511229-105521053793246
ENST00000549338SLC25A3chr1298987913+ENST00000320356EZH2chr7148511229-105521053793246
ENST00000549338SLC25A3chr1298987913+ENST00000541220EZH2chr7148511229-105521053793246
ENST00000549338SLC25A3chr1298987913+ENST00000476773EZH2chr7148511229-95821053793246
ENST00000549338SLC25A3chr1298987913+ENST00000483967EZH2chr7148511229-91521053793246
ENST00000548847SLC25A3chr1298987913+ENST00000478654EZH2chr7148511229-125741138994318
ENST00000548847SLC25A3chr1298987913+ENST00000460911EZH2chr7148511229-125641138994318
ENST00000548847SLC25A3chr1298987913+ENST00000350995EZH2chr7148511229-125641138994318
ENST00000548847SLC25A3chr1298987913+ENST00000320356EZH2chr7148511229-125641138994318
ENST00000548847SLC25A3chr1298987913+ENST00000541220EZH2chr7148511229-125641138994318
ENST00000548847SLC25A3chr1298987913+ENST00000476773EZH2chr7148511229-115941138994318
ENST00000548847SLC25A3chr1298987913+ENST00000483967EZH2chr7148511229-111641138994318

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000401722ENST00000478654SLC25A3chr1298987913+EZH2chr7148511229-0.002455310.9975447
ENST00000401722ENST00000460911SLC25A3chr1298987913+EZH2chr7148511229-0.0024759260.9975241
ENST00000401722ENST00000350995SLC25A3chr1298987913+EZH2chr7148511229-0.0024759260.9975241
ENST00000401722ENST00000320356SLC25A3chr1298987913+EZH2chr7148511229-0.0024759260.9975241
ENST00000401722ENST00000541220SLC25A3chr1298987913+EZH2chr7148511229-0.0024759260.9975241
ENST00000401722ENST00000476773SLC25A3chr1298987913+EZH2chr7148511229-0.0029046990.99709535
ENST00000401722ENST00000483967SLC25A3chr1298987913+EZH2chr7148511229-0.0032565220.9967435
ENST00000228318ENST00000478654SLC25A3chr1298987913+EZH2chr7148511229-0.0025117070.99748826
ENST00000228318ENST00000460911SLC25A3chr1298987913+EZH2chr7148511229-0.002530190.9974698
ENST00000228318ENST00000350995SLC25A3chr1298987913+EZH2chr7148511229-0.002530190.9974698
ENST00000228318ENST00000320356SLC25A3chr1298987913+EZH2chr7148511229-0.002530190.9974698
ENST00000228318ENST00000541220SLC25A3chr1298987913+EZH2chr7148511229-0.002530190.9974698
ENST00000228318ENST00000476773SLC25A3chr1298987913+EZH2chr7148511229-0.0029508490.99704915
ENST00000228318ENST00000483967SLC25A3chr1298987913+EZH2chr7148511229-0.003343460.99665654
ENST00000188376ENST00000478654SLC25A3chr1298987913+EZH2chr7148511229-0.0029518280.9970482
ENST00000188376ENST00000460911SLC25A3chr1298987913+EZH2chr7148511229-0.0029837790.9970162
ENST00000188376ENST00000350995SLC25A3chr1298987913+EZH2chr7148511229-0.0029837790.9970162
ENST00000188376ENST00000320356SLC25A3chr1298987913+EZH2chr7148511229-0.0029837790.9970162
ENST00000188376ENST00000541220SLC25A3chr1298987913+EZH2chr7148511229-0.0029837790.9970162
ENST00000188376ENST00000476773SLC25A3chr1298987913+EZH2chr7148511229-0.0033294210.99667054
ENST00000188376ENST00000483967SLC25A3chr1298987913+EZH2chr7148511229-0.0037883590.99621165
ENST00000551917ENST00000478654SLC25A3chr1298987913+EZH2chr7148511229-0.002481430.99751854
ENST00000551917ENST00000460911SLC25A3chr1298987913+EZH2chr7148511229-0.0024889940.997511
ENST00000551917ENST00000350995SLC25A3chr1298987913+EZH2chr7148511229-0.0024889940.997511
ENST00000551917ENST00000320356SLC25A3chr1298987913+EZH2chr7148511229-0.0024889940.997511
ENST00000551917ENST00000541220SLC25A3chr1298987913+EZH2chr7148511229-0.0024889940.997511
ENST00000551917ENST00000476773SLC25A3chr1298987913+EZH2chr7148511229-0.002913090.99708694
ENST00000551917ENST00000483967SLC25A3chr1298987913+EZH2chr7148511229-0.0031331320.9968669
ENST00000552981ENST00000478654SLC25A3chr1298987913+EZH2chr7148511229-0.0025400410.99745995
ENST00000552981ENST00000460911SLC25A3chr1298987913+EZH2chr7148511229-0.0025434380.9974566
ENST00000552981ENST00000350995SLC25A3chr1298987913+EZH2chr7148511229-0.0025434380.9974566
ENST00000552981ENST00000320356SLC25A3chr1298987913+EZH2chr7148511229-0.0025434380.9974566
ENST00000552981ENST00000541220SLC25A3chr1298987913+EZH2chr7148511229-0.0025434380.9974566
ENST00000552981ENST00000476773SLC25A3chr1298987913+EZH2chr7148511229-0.002981750.9970182
ENST00000552981ENST00000483967SLC25A3chr1298987913+EZH2chr7148511229-0.0031759160.99682415
ENST00000547534ENST00000478654SLC25A3chr1298987913+EZH2chr7148511229-0.0027107540.9972893
ENST00000547534ENST00000460911SLC25A3chr1298987913+EZH2chr7148511229-0.0027186080.9972813
ENST00000547534ENST00000350995SLC25A3chr1298987913+EZH2chr7148511229-0.0027186080.9972813
ENST00000547534ENST00000320356SLC25A3chr1298987913+EZH2chr7148511229-0.0027186080.9972813
ENST00000547534ENST00000541220SLC25A3chr1298987913+EZH2chr7148511229-0.0027186080.9972813
ENST00000547534ENST00000476773SLC25A3chr1298987913+EZH2chr7148511229-0.0032101060.9967899
ENST00000547534ENST00000483967SLC25A3chr1298987913+EZH2chr7148511229-0.0034878020.99651223
ENST00000549338ENST00000478654SLC25A3chr1298987913+EZH2chr7148511229-0.0028094420.99719054
ENST00000549338ENST00000460911SLC25A3chr1298987913+EZH2chr7148511229-0.0028157820.9971842
ENST00000549338ENST00000350995SLC25A3chr1298987913+EZH2chr7148511229-0.0028157820.9971842
ENST00000549338ENST00000320356SLC25A3chr1298987913+EZH2chr7148511229-0.0028157820.9971842
ENST00000549338ENST00000541220SLC25A3chr1298987913+EZH2chr7148511229-0.0028157820.9971842
ENST00000549338ENST00000476773SLC25A3chr1298987913+EZH2chr7148511229-0.0033571330.9966428
ENST00000549338ENST00000483967SLC25A3chr1298987913+EZH2chr7148511229-0.0037016640.9962984
ENST00000548847ENST00000478654SLC25A3chr1298987913+EZH2chr7148511229-0.0028661690.99713385
ENST00000548847ENST00000460911SLC25A3chr1298987913+EZH2chr7148511229-0.0029029190.99709713
ENST00000548847ENST00000350995SLC25A3chr1298987913+EZH2chr7148511229-0.0029029190.99709713
ENST00000548847ENST00000320356SLC25A3chr1298987913+EZH2chr7148511229-0.0029029190.99709713
ENST00000548847ENST00000541220SLC25A3chr1298987913+EZH2chr7148511229-0.0029029190.99709713
ENST00000548847ENST00000476773SLC25A3chr1298987913+EZH2chr7148511229-0.0033794730.99662054
ENST00000548847ENST00000483967SLC25A3chr1298987913+EZH2chr7148511229-0.0039510040.996049

check button Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones.

Get the fusion protein sequences from here.

Fusion protein sequence information is available in the fasta format.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

Top

Fusion Protein Breakpoint Sequences for SLC25A3-EZH2

check button +/-13 AA sequence from the breakpoints of the fusion protein sequences.
HgeneHchrHbpTgeneTchrTbpLength(fusion protein)BP in fusion proteinPeptide
SLC25A3chr1298987913EZH2chr714851122921051QPRRPRNLAAAAVEGQNRFPGCRCKA
SLC25A3chr1298987913EZH2chr714851122921660QPRRPRNLAAAAVEGQNRFPGCRCKA
SLC25A3chr1298987913EZH2chr714851122924260QPRRPRNLAAAAVEGQNRFPGCRCKA
SLC25A3chr1298987913EZH2chr714851122924851QPRRPRNLAAAAVEGQNRFPGCRCKA
SLC25A3chr1298987913EZH2chr714851122927760QPRRPRNLAAAAVEGQNRFPGCRCKA
SLC25A3chr1298987913EZH2chr714851122930751QPRRPRNLAAAAVEGQNRFPGCRCKA
SLC25A3chr1298987913EZH2chr7148511229411123QPRRPRNLAAAAVEGQNRFPGCRCKA
SLC25A3chr1298987913EZH2chr7148511229511123QPRRPRNLAAAAVEGQNRFPGCRCKA

Top

Potential FusionNeoAntigen Information of SLC25A3-EZH2 in HLA I

check button Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.
SLC25A3-EZH2_98987913_148511229.msa

check button Potential FusionNeoAntigen Information
* We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5)
Fusion geneHchrHbpTgeneTchrTbpHLA IFusionNeoAntigen peptideBinding scoreImmunogenic scoreNeoantigen start (at BP 13)Neoantigen end (at BP 13)
SLC25A3-EZH2chr1298987913chr7148511229511HLA-B15:17AAVEGQNRF0.98860.96561019
SLC25A3-EZH2chr1298987913chr7148511229511HLA-B15:16AAVEGQNRF0.98670.9031019
SLC25A3-EZH2chr1298987913chr7148511229511HLA-B46:01AAVEGQNRF0.96150.65721019
SLC25A3-EZH2chr1298987913chr7148511229511HLA-B13:01AAVEGQNRF0.12910.98691019
SLC25A3-EZH2chr1298987913chr7148511229511HLA-C03:08AAVEGQNRF0.99660.9391019
SLC25A3-EZH2chr1298987913chr7148511229511HLA-C03:19AAVEGQNRF0.99470.9911019
SLC25A3-EZH2chr1298987913chr7148511229511HLA-B15:05AAVEGQNRF0.99060.9661019
SLC25A3-EZH2chr1298987913chr7148511229511HLA-C15:04AAVEGQNRF0.9850.96561019
SLC25A3-EZH2chr1298987913chr7148511229511HLA-C03:07AAVEGQNRF0.95970.99191019
SLC25A3-EZH2chr1298987913chr7148511229511HLA-C08:13AAVEGQNRF0.77680.98851019
SLC25A3-EZH2chr1298987913chr7148511229511HLA-C08:04AAVEGQNRF0.77680.98851019
SLC25A3-EZH2chr1298987913chr7148511229511HLA-C03:14AAVEGQNRF0.72590.98221019
SLC25A3-EZH2chr1298987913chr7148511229511HLA-C12:04AAVEGQNRF0.70710.99541019
SLC25A3-EZH2chr1298987913chr7148511229511HLA-C12:12AAVEGQNRF0.70050.94541019
SLC25A3-EZH2chr1298987913chr7148511229511HLA-C06:03AAVEGQNRF0.69780.9951019
SLC25A3-EZH2chr1298987913chr7148511229511HLA-C02:06AAVEGQNRF0.53350.97571019
SLC25A3-EZH2chr1298987913chr7148511229511HLA-C08:03AAVEGQNRF0.43670.99461019
SLC25A3-EZH2chr1298987913chr7148511229511HLA-C03:02AAVEGQNRF0.99710.97991019
SLC25A3-EZH2chr1298987913chr7148511229511HLA-C03:03AAVEGQNRF0.99650.99061019
SLC25A3-EZH2chr1298987913chr7148511229511HLA-C03:04AAVEGQNRF0.99650.99061019
SLC25A3-EZH2chr1298987913chr7148511229511HLA-B35:11AAVEGQNRF0.99140.96591019
SLC25A3-EZH2chr1298987913chr7148511229511HLA-B15:20AAVEGQNRF0.99090.97841019
SLC25A3-EZH2chr1298987913chr7148511229511HLA-B35:28AAVEGQNRF0.98950.98041019
SLC25A3-EZH2chr1298987913chr7148511229511HLA-B58:06AAVEGQNRF0.98770.97561019
SLC25A3-EZH2chr1298987913chr7148511229511HLA-B57:02AAVEGQNRF0.98660.98171019
SLC25A3-EZH2chr1298987913chr7148511229511HLA-C15:09AAVEGQNRF0.9850.96561019
SLC25A3-EZH2chr1298987913chr7148511229511HLA-C03:05AAVEGQNRF0.97090.92631019
SLC25A3-EZH2chr1298987913chr7148511229511HLA-C03:17AAVEGQNRF0.97050.97261019
SLC25A3-EZH2chr1298987913chr7148511229511HLA-C16:04AAVEGQNRF0.93260.98661019
SLC25A3-EZH2chr1298987913chr7148511229511HLA-B15:08AAVEGQNRF0.92630.94991019
SLC25A3-EZH2chr1298987913chr7148511229511HLA-B15:24AAVEGQNRF0.92420.96351019
SLC25A3-EZH2chr1298987913chr7148511229511HLA-B35:13AAVEGQNRF0.91160.97211019
SLC25A3-EZH2chr1298987913chr7148511229511HLA-B35:43AAVEGQNRF0.9080.95141019
SLC25A3-EZH2chr1298987913chr7148511229511HLA-C03:06AAVEGQNRF0.82240.99151019
SLC25A3-EZH2chr1298987913chr7148511229511HLA-C12:02AAVEGQNRF0.80090.97861019
SLC25A3-EZH2chr1298987913chr7148511229511HLA-C12:03AAVEGQNRF0.75150.98681019
SLC25A3-EZH2chr1298987913chr7148511229511HLA-C16:02AAVEGQNRF0.64650.99581019
SLC25A3-EZH2chr1298987913chr7148511229511HLA-C16:01AAVEGQNRF0.64240.98931019
SLC25A3-EZH2chr1298987913chr7148511229511HLA-C02:10AAVEGQNRF0.57980.98511019
SLC25A3-EZH2chr1298987913chr7148511229511HLA-C02:02AAVEGQNRF0.57980.98511019
SLC25A3-EZH2chr1298987913chr7148511229511HLA-C08:01AAVEGQNRF0.43670.99461019

Top

Potential FusionNeoAntigen Information of SLC25A3-EZH2 in HLA II

check button Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.
SLC25A3-EZH2_98987913_148511229.msa

check button Potential FusionNeoAntigen Information
* We used NetMHCIIpan v4.1 (%rank<0.5).
Fusion geneHchrHbpTgeneTchrTbpHLA IIFusionNeoAntigen peptideNeoantigen start (at BP 13)Neoantigen end (at BP 13)
SLC25A3-EZH2chr1298987913chr7148511229511DRB1-0451RPRNLAAAAVEGQNR318
SLC25A3-EZH2chr1298987913chr7148511229511DRB1-0451PRNLAAAAVEGQNRF419
SLC25A3-EZH2chr1298987913chr7148511229511DRB1-0478RPRNLAAAAVEGQNR318
SLC25A3-EZH2chr1298987913chr7148511229511DRB1-0478PRNLAAAAVEGQNRF419

Top

Fusion breakpoint peptide structures of SLC25A3-EZH2

check button3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens
* The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA.
File nameBPseqHgeneTgeneHchrHbpTchrTbpAAlen
6238NLAAAAVEGQNRFPSLC25A3EZH2chr1298987913chr7148511229511

Top

Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of SLC25A3-EZH2

check buttonVirtual screening between 25 HLAs (from PDB) and FusionNeoAntigens
* We used Glide to predict the interaction between HLAs and neoantigens.
HLA allelePDB IDFile nameBPseqDocking scoreGlide score
HLA-B14:023BVN6238NLAAAAVEGQNRFP-7.9962-8.1096
HLA-B14:023BVN6238NLAAAAVEGQNRFP-5.70842-6.74372
HLA-B52:013W396238NLAAAAVEGQNRFP-6.83737-6.95077
HLA-B52:013W396238NLAAAAVEGQNRFP-4.4836-5.5189
HLA-A11:014UQ26238NLAAAAVEGQNRFP-10.0067-10.1201
HLA-A11:014UQ26238NLAAAAVEGQNRFP-9.03915-10.0745
HLA-A24:025HGA6238NLAAAAVEGQNRFP-6.56204-6.67544
HLA-A24:025HGA6238NLAAAAVEGQNRFP-5.42271-6.45801
HLA-B44:053DX86238NLAAAAVEGQNRFP-7.85648-8.89178
HLA-B44:053DX86238NLAAAAVEGQNRFP-5.3978-5.5112
HLA-A02:016TDR6238NLAAAAVEGQNRFP-3.37154-4.40684

Top

Vaccine Design for the FusionNeoAntigens of SLC25A3-EZH2

check button mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is.
Fusion geneHchrHbpTchrTbpStart in +/-13AAEnd in +/-13AAFusionNeoAntigen peptide sequenceFusionNeoAntigen RNA sequence
SLC25A3-EZH2chr1298987913chr71485112291019AAVEGQNRFCCGTGGAAGGTCAAAACCGCTTTCCGG

check button mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs.
Fusion geneHchrHbpTchrTbpStart in +/-13AAEnd in +/-13AAFusionNeoAntigen peptideFusionNEoAntigen RNA sequence
SLC25A3-EZH2chr1298987913chr7148511229318RPRNLAAAAVEGQNRCTCGCAACCTGGCAGCCGCCGCCGTGGAAGGTCAAAACCGCTTTC
SLC25A3-EZH2chr1298987913chr7148511229419PRNLAAAAVEGQNRFGCAACCTGGCAGCCGCCGCCGTGGAAGGTCAAAACCGCTTTCCGG

Top

Information of the samples that have these potential fusion neoantigens of SLC25A3-EZH2

check button These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens.
Cancer typeFusion geneHchrHbpHenstTchrTbpTenstSample
STADSLC25A3-EZH2chr1298987913ENST00000188376chr7148511229ENST00000320356TCGA-BR-8081

Top

Potential target of CAR-T therapy development for SLC25A3-EZH2

check button Predicted 3D structure. We used RoseTTAFold.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features


* Minus value of BPloci means that the break point is located before the CDS.
- In-frame and retained 'Transmembrane'.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

check button Subcellular localization prediction of the transmembrane domain retained fusion proteins
* We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image.
HgeneHchrHbpHenstTgeneTchrTbpTenstDeepLoc result

Top

Related Drugs to SLC25A3-EZH2

check button Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDrugSourcePMID

Top

Related Diseases to SLC25A3-EZH2

check button Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDiseaseSourcePMID

check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource