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Fusion Protein:SMCHD1-DLGAP1

Fusion Gene and Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: SMCHD1-DLGAP1
	FusionPDB ID: 84026
	FusionGDB2.0 ID: 84026
		Hgene	Tgene
	Gene symbol	SMCHD1	DLGAP1
	Gene ID	23347	9229
	Gene name	structural maintenance of chromosomes flexible hinge domain containing 1	DLG associated protein 1
	Synonyms	BAMS\|FSHD2	DAP-1\|DAP-1-ALPHA\|DAP-1-BETA\|DAP1\|DLGAP1A\|DLGAP1B\|GKAP\|SAPAP1
	Cytomap	18p11.32	18p11.31
	Type of gene	protein-coding	protein-coding
	Description	structural maintenance of chromosomes flexible hinge domain-containing protein 1SMC hinge domain-containing protein 1	disks large-associated protein 1PSD-95/SAP90 binding protein 1SAP90/PSD-95-associated protein 1discs large homolog associated protein 1guanylate kinase-associated protein
	Modification date	20200313	20200313
	UniProtAcc	.	O14490 Main function of 5'-partner protein: FUNCTION: Part of the postsynaptic scaffold in neuronal cells.
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000261598, ENST00000320876, ENST00000609587,	ENST00000478161, ENST00000315677, ENST00000400145, ENST00000400147, ENST00000400149, ENST00000400150, ENST00000400155, ENST00000515196, ENST00000534970, ENST00000539435, ENST00000581527, ENST00000581699, ENST00000584874,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	6 X 9 X 6=324	31 X 20 X 8=4960
	# samples	11	32
	** MAII score	log2(11/324*10)=-1.55849028935997 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(32/4960*10)=-3.95419631038688 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Fusion gene context	PubMed: SMCHD1 [Title/Abstract] AND DLGAP1 [Title/Abstract] AND fusion [Title/Abstract]
Fusion neoantigen context	PubMed: SMCHD1 [Title/Abstract] AND DLGAP1 [Title/Abstract] AND neoantigen [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	SMCHD1(2674144)-DLGAP1(3742512), # samples:1
Anticipated loss of major functional domain due to fusion event.	SMCHD1-DLGAP1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. SMCHD1-DLGAP1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. SMCHD1-DLGAP1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. SMCHD1-DLGAP1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	SMCHD1	GO:0009048	dosage compensation by inactivation of X chromosome	23542155

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr18:2674144/chr18:3742512)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Retention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here.

Fusion gene breakpoints across SMCHD1 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across DLGAP1 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Amino Acid Sequences

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000320876	SMCHD1	chr18	2674144	+	ENST00000315677	DLGAP1	chr18	3742512	-	5891	976	314	2737	807
ENST00000320876	SMCHD1	chr18	2674144	+	ENST00000581527	DLGAP1	chr18	3742512	-	2802	976	314	2593	759
ENST00000320876	SMCHD1	chr18	2674144	+	ENST00000400147	DLGAP1	chr18	3742512	-	5891	976	314	2737	807
ENST00000320876	SMCHD1	chr18	2674144	+	ENST00000400149	DLGAP1	chr18	3742512	-	5837	976	314	2683	789
ENST00000320876	SMCHD1	chr18	2674144	+	ENST00000400155	DLGAP1	chr18	3742512	-	5891	976	314	2737	807
ENST00000320876	SMCHD1	chr18	2674144	+	ENST00000400150	DLGAP1	chr18	3742512	-	5921	976	314	2767	817
ENST00000320876	SMCHD1	chr18	2674144	+	ENST00000584874	DLGAP1	chr18	3742512	-	3084	976	314	2737	807
ENST00000320876	SMCHD1	chr18	2674144	+	ENST00000534970	DLGAP1	chr18	3742512	-	2972	976	314	2653	779
ENST00000320876	SMCHD1	chr18	2674144	+	ENST00000539435	DLGAP1	chr18	3742512	-	3036	976	314	2767	817
ENST00000320876	SMCHD1	chr18	2674144	+	ENST00000581699	DLGAP1	chr18	3742512	-	2919	976	314	2737	807
ENST00000320876	SMCHD1	chr18	2674144	+	ENST00000400145	DLGAP1	chr18	3742512	-	2884	976	314	2593	759
ENST00000320876	SMCHD1	chr18	2674144	+	ENST00000515196	DLGAP1	chr18	3742512	-	2802	976	314	2593	759
ENST00000261598	SMCHD1	chr18	2674144	+	ENST00000315677	DLGAP1	chr18	3742512	-	5742	827	165	2588	807
ENST00000261598	SMCHD1	chr18	2674144	+	ENST00000581527	DLGAP1	chr18	3742512	-	2653	827	165	2444	759
ENST00000261598	SMCHD1	chr18	2674144	+	ENST00000400147	DLGAP1	chr18	3742512	-	5742	827	165	2588	807
ENST00000261598	SMCHD1	chr18	2674144	+	ENST00000400149	DLGAP1	chr18	3742512	-	5688	827	165	2534	789
ENST00000261598	SMCHD1	chr18	2674144	+	ENST00000400155	DLGAP1	chr18	3742512	-	5742	827	165	2588	807
ENST00000261598	SMCHD1	chr18	2674144	+	ENST00000400150	DLGAP1	chr18	3742512	-	5772	827	165	2618	817
ENST00000261598	SMCHD1	chr18	2674144	+	ENST00000584874	DLGAP1	chr18	3742512	-	2935	827	165	2588	807
ENST00000261598	SMCHD1	chr18	2674144	+	ENST00000534970	DLGAP1	chr18	3742512	-	2823	827	165	2504	779
ENST00000261598	SMCHD1	chr18	2674144	+	ENST00000539435	DLGAP1	chr18	3742512	-	2887	827	165	2618	817
ENST00000261598	SMCHD1	chr18	2674144	+	ENST00000581699	DLGAP1	chr18	3742512	-	2770	827	165	2588	807
ENST00000261598	SMCHD1	chr18	2674144	+	ENST00000400145	DLGAP1	chr18	3742512	-	2735	827	165	2444	759
ENST00000261598	SMCHD1	chr18	2674144	+	ENST00000515196	DLGAP1	chr18	3742512	-	2653	827	165	2444	759

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000320876	ENST00000315677	SMCHD1	chr18	2674144	+	DLGAP1	chr18	3742512	-	0.000296473	0.99970347
ENST00000320876	ENST00000581527	SMCHD1	chr18	2674144	+	DLGAP1	chr18	3742512	-	0.001177734	0.9988223
ENST00000320876	ENST00000400147	SMCHD1	chr18	2674144	+	DLGAP1	chr18	3742512	-	0.000296473	0.99970347
ENST00000320876	ENST00000400149	SMCHD1	chr18	2674144	+	DLGAP1	chr18	3742512	-	0.000261249	0.99973875
ENST00000320876	ENST00000400155	SMCHD1	chr18	2674144	+	DLGAP1	chr18	3742512	-	0.000296473	0.99970347
ENST00000320876	ENST00000400150	SMCHD1	chr18	2674144	+	DLGAP1	chr18	3742512	-	0.000264829	0.9997352
ENST00000320876	ENST00000584874	SMCHD1	chr18	2674144	+	DLGAP1	chr18	3742512	-	0.003068574	0.99693143
ENST00000320876	ENST00000534970	SMCHD1	chr18	2674144	+	DLGAP1	chr18	3742512	-	0.003465761	0.9965342
ENST00000320876	ENST00000539435	SMCHD1	chr18	2674144	+	DLGAP1	chr18	3742512	-	0.003020669	0.99697936
ENST00000320876	ENST00000581699	SMCHD1	chr18	2674144	+	DLGAP1	chr18	3742512	-	0.003388249	0.9966118
ENST00000320876	ENST00000400145	SMCHD1	chr18	2674144	+	DLGAP1	chr18	3742512	-	0.001183547	0.9988165
ENST00000320876	ENST00000515196	SMCHD1	chr18	2674144	+	DLGAP1	chr18	3742512	-	0.001177734	0.9988223
ENST00000261598	ENST00000315677	SMCHD1	chr18	2674144	+	DLGAP1	chr18	3742512	-	0.000302241	0.9996978
ENST00000261598	ENST00000581527	SMCHD1	chr18	2674144	+	DLGAP1	chr18	3742512	-	0.001295531	0.99870443
ENST00000261598	ENST00000400147	SMCHD1	chr18	2674144	+	DLGAP1	chr18	3742512	-	0.000302241	0.9996978
ENST00000261598	ENST00000400149	SMCHD1	chr18	2674144	+	DLGAP1	chr18	3742512	-	0.00026433	0.99973565
ENST00000261598	ENST00000400155	SMCHD1	chr18	2674144	+	DLGAP1	chr18	3742512	-	0.000302241	0.9996978
ENST00000261598	ENST00000400150	SMCHD1	chr18	2674144	+	DLGAP1	chr18	3742512	-	0.000270186	0.9997298
ENST00000261598	ENST00000584874	SMCHD1	chr18	2674144	+	DLGAP1	chr18	3742512	-	0.003483809	0.9965162
ENST00000261598	ENST00000534970	SMCHD1	chr18	2674144	+	DLGAP1	chr18	3742512	-	0.00404371	0.99595624
ENST00000261598	ENST00000539435	SMCHD1	chr18	2674144	+	DLGAP1	chr18	3742512	-	0.003471042	0.9965289
ENST00000261598	ENST00000581699	SMCHD1	chr18	2674144	+	DLGAP1	chr18	3742512	-	0.003949208	0.9960508
ENST00000261598	ENST00000400145	SMCHD1	chr18	2674144	+	DLGAP1	chr18	3742512	-	0.001307725	0.99869233
ENST00000261598	ENST00000515196	SMCHD1	chr18	2674144	+	DLGAP1	chr18	3742512	-	0.001295531	0.99870443

Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones.

Get the fusion protein sequences from here.

Fusion protein sequence information is available in the fasta format.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

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Fusion Protein Breakpoint Sequences for SMCHD1-DLGAP1

+/-13 AA sequence from the breakpoints of the fusion protein sequences.

Hgene	Hchr	Hbp	Tgene	Tchr	Tbp	Length(fusion protein)	BP in fusion protein	Peptide
SMCHD1	chr18	2674144	DLGAP1	chr18	3742512	827	220	YRLSKFTRQGDFERISNEHSPKLQIR
SMCHD1	chr18	2674144	DLGAP1	chr18	3742512	976	220	YRLSKFTRQGDFERISNEHSPKLQIR

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Potential FusionNeoAntigen Information of SMCHD1-DLGAP1 in HLA I

Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.

SMCHD1-DLGAP1_2674144_3742512.msa

Potential FusionNeoAntigen Information
* We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5)

Fusion gene	Hchr	Hbp	Tgene	Tchr	Tbp	HLA I	FusionNeoAntigen peptide	Binding score	Immunogenic score	Neoantigen start (at BP 13)	Neoantigen end (at BP 13)
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	HLA-B39:06	ERISNEHSP	0.9782	0.7271	12	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	HLA-B38:01	TRQGDFERI	0.9681	0.934	6	15
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	HLA-B38:02	TRQGDFERI	0.9656	0.9316	6	15
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	HLA-B39:01	ERISNEHSPKL	0.9993	0.8667	12	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	HLA-B73:01	ERISNEHSP	0.982	0.5117	12	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	HLA-B73:01	FERISNEHSP	0.9675	0.5305	11	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	HLA-B39:12	ERISNEHSPKL	0.9991	0.8735	12	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	HLA-B18:06	FERISNEH	0.9975	0.9239	11	19
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	HLA-B38:05	TRQGDFERI	0.9681	0.934	6	15
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	HLA-B39:31	ERISNEHSPKL	0.9993	0.8659	12	23

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Potential FusionNeoAntigen Information of SMCHD1-DLGAP1 in HLA II

SMCHD1-DLGAP1_2674144_3742512.msa

Potential FusionNeoAntigen Information
* We used NetMHCIIpan v4.1 (%rank<0.5).

Fusion gene	Hchr	Hbp	Tgene	Tchr	Tbp	HLA II	FusionNeoAntigen peptide	Neoantigen start (at BP 13)	Neoantigen end (at BP 13)
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0109	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0109	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0113	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0113	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0401	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0401	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0401	TRQGDFERISNEHSP	6	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0401	GDFERISNEHSPKLQ	9	24
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0403	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0403	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0403	TRQGDFERISNEHSP	6	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0405	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0405	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0405	TRQGDFERISNEHSP	6	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0407	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0407	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0407	TRQGDFERISNEHSP	6	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0407	GDFERISNEHSPKLQ	9	24
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0409	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0409	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0409	TRQGDFERISNEHSP	6	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0413	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0413	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0415	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0415	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0417	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0417	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0417	TRQGDFERISNEHSP	6	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0419	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0419	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0419	TRQGDFERISNEHSP	6	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0419	GDFERISNEHSPKLQ	9	24
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0424	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0424	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0424	TRQGDFERISNEHSP	6	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0427	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0427	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0427	TRQGDFERISNEHSP	6	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0429	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0429	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0429	TRQGDFERISNEHSP	6	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0430	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0430	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0430	TRQGDFERISNEHSP	6	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0431	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0431	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0431	TRQGDFERISNEHSP	6	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0431	GDFERISNEHSPKLQ	9	24
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0433	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0433	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0433	TRQGDFERISNEHSP	6	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0433	GDFERISNEHSPKLQ	9	24
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0434	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0434	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0434	TRQGDFERISNEHSP	6	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0434	GDFERISNEHSPKLQ	9	24
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0435	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0435	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0435	TRQGDFERISNEHSP	6	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0435	GDFERISNEHSPKLQ	9	24
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0436	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0436	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0438	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0438	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0438	TRQGDFERISNEHSP	6	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0438	GDFERISNEHSPKLQ	9	24
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0439	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0439	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0439	TRQGDFERISNEHSP	6	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0441	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0441	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0441	TRQGDFERISNEHSP	6	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0443	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0443	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0443	TRQGDFERISNEHSP	6	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0443	GDFERISNEHSPKLQ	9	24
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0444	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0444	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0445	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0445	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0445	TRQGDFERISNEHSP	6	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0446	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0446	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0446	TRQGDFERISNEHSP	6	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0447	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0447	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0447	TRQGDFERISNEHSP	6	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0447	GDFERISNEHSPKLQ	9	24
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0448	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0448	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0448	TRQGDFERISNEHSP	6	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0449	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0449	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0449	TRQGDFERISNEHSP	6	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0450	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0450	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0450	TRQGDFERISNEHSP	6	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0451	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0451	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0451	TRQGDFERISNEHSP	6	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0452	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0452	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0452	TRQGDFERISNEHSP	6	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0453	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0453	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0454	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0454	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0454	TRQGDFERISNEHSP	6	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0454	GDFERISNEHSPKLQ	9	24
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0457	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0457	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0457	TRQGDFERISNEHSP	6	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0458	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0459	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0459	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0460	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0460	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0460	TRQGDFERISNEHSP	6	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0461	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0461	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0461	TRQGDFERISNEHSP	6	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0461	GDFERISNEHSPKLQ	9	24
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0462	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0462	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0462	TRQGDFERISNEHSP	6	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0463	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0463	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0463	TRQGDFERISNEHSP	6	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0463	GDFERISNEHSPKLQ	9	24
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0464	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0464	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0464	TRQGDFERISNEHSP	6	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0464	GDFERISNEHSPKLQ	9	24
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0465	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0465	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0466	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0466	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0469	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0469	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0469	TRQGDFERISNEHSP	6	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0470	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0470	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0471	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0471	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0471	TRQGDFERISNEHSP	6	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0472	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0472	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0472	TRQGDFERISNEHSP	6	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0472	GDFERISNEHSPKLQ	9	24
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0474	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0474	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0474	TRQGDFERISNEHSP	6	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0474	GDFERISNEHSPKLQ	9	24
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0475	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0475	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0475	TRQGDFERISNEHSP	6	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0475	GDFERISNEHSPKLQ	9	24
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0476	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0476	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0476	TRQGDFERISNEHSP	6	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0476	GDFERISNEHSPKLQ	9	24
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0477	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0477	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0477	TRQGDFERISNEHSP	6	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0478	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0478	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0479	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0479	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0480	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0480	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0480	TRQGDFERISNEHSP	6	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0482	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0482	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0482	TRQGDFERISNEHSP	6	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0483	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0483	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0483	TRQGDFERISNEHSP	6	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0484	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0484	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0484	TRQGDFERISNEHSP	6	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0485	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0485	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0485	TRQGDFERISNEHSP	6	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0486	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0486	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0487	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0487	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0487	TRQGDFERISNEHSP	6	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0488	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0488	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0488	TRQGDFERISNEHSP	6	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0489	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0489	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0489	TRQGDFERISNEHSP	6	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0813	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0813	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0815	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0824	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0824	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-0830	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1001	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1001	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1001	TRQGDFERISNEHSP	6	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1003	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1003	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1003	TRQGDFERISNEHSP	6	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1130	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1130	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1367	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1367	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1402	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1402	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1409	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1409	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1441	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1441	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1446	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1446	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1446	TRQGDFERISNEHSP	6	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1494	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1494	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1511	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1511	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1515	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1515	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1525	FERISNEHSPKLQIR	11	26
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1525	DFERISNEHSPKLQI	10	25
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1525	GDFERISNEHSPKLQ	9	24
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1527	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1527	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1531	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1531	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1534	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1534	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1601	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1601	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1601	TRQGDFERISNEHSP	6	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1602	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1602	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1603	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1603	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1603	TRQGDFERISNEHSP	6	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1604	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1604	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1605	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1605	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1607	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1607	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1608	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1608	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1608	TRQGDFERISNEHSP	6	21
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1609	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1609	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1610	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1610	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1611	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1611	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1612	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1612	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1614	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1614	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1616	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB1-1616	QGDFERISNEHSPKL	8	23
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB3-0217	RQGDFERISNEHSPK	7	22
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	827	DRB3-0217	QGDFERISNEHSPKL	8	23

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Fusion breakpoint peptide structures of SMCHD1-DLGAP1

3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens
* The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA.

File name	BPseq	Hgene	Tgene	Hchr	Hbp	Tchr	Tbp	AAlen
9608	TRQGDFERISNEHS	SMCHD1	DLGAP1	chr18	2674144	chr18	3742512	827

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of SMCHD1-DLGAP1

Virtual screening between 25 HLAs (from PDB) and FusionNeoAntigens
* We used Glide to predict the interaction between HLAs and neoantigens.

HLA allele	PDB ID	File name	BPseq	Docking score	Glide score
HLA-B14:02	3BVN	9608	TRQGDFERISNEHS	-7.15543	-7.26883
HLA-B14:02	3BVN	9608	TRQGDFERISNEHS	-4.77435	-5.80965
HLA-B52:01	3W39	9608	TRQGDFERISNEHS	-6.80875	-6.92215
HLA-B52:01	3W39	9608	TRQGDFERISNEHS	-4.20386	-5.23916
HLA-A11:01	4UQ2	9608	TRQGDFERISNEHS	-7.5194	-8.5547
HLA-A11:01	4UQ2	9608	TRQGDFERISNEHS	-6.9601	-7.0735
HLA-A24:02	5HGA	9608	TRQGDFERISNEHS	-7.52403	-7.63743
HLA-A24:02	5HGA	9608	TRQGDFERISNEHS	-5.82433	-6.85963
HLA-B27:05	6PYJ	9608	TRQGDFERISNEHS	-3.28285	-4.31815
HLA-B44:05	3DX8	9608	TRQGDFERISNEHS	-5.91172	-6.94702
HLA-B44:05	3DX8	9608	TRQGDFERISNEHS	-4.24346	-4.35686

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Vaccine Design for the FusionNeoAntigens of SMCHD1-DLGAP1

mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is.

Fusion gene	Hchr	Hbp	Tchr	Tbp	Start in +/-13AA	End in +/-13AA	FusionNeoAntigen peptide sequence	FusionNeoAntigen RNA sequence
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	11	19	FERISNEH	TGAAAGAATCTCCAATGAACACTC
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	11	21	FERISNEHSP	TGAAAGAATCTCCAATGAACACTCACCCAA
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	12	21	ERISNEHSP	AAGAATCTCCAATGAACACTCACCCAA
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	12	23	ERISNEHSPKL	AAGAATCTCCAATGAACACTCACCCAAACTCCA
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	6	15	TRQGDFERI	AAGGCAAGGTGACTTTGAAAGAATCTC

mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs.

Fusion gene	Hchr	Hbp	Tchr	Tbp	Start in +/-13AA	End in +/-13AA	FusionNeoAntigen peptide	FusionNEoAntigen RNA sequence
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	10	25	DFERISNEHSPKLQI	CTTTGAAAGAATCTCCAATGAACACTCACCCAAACTCCAGATCCG
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	11	26	FERISNEHSPKLQIR	TGAAAGAATCTCCAATGAACACTCACCCAAACTCCAGATCCGGAG
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	6	21	TRQGDFERISNEHSP	AAGGCAAGGTGACTTTGAAAGAATCTCCAATGAACACTCACCCAA
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	7	22	RQGDFERISNEHSPK	GCAAGGTGACTTTGAAAGAATCTCCAATGAACACTCACCCAAACT
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	8	23	QGDFERISNEHSPKL	AGGTGACTTTGAAAGAATCTCCAATGAACACTCACCCAAACTCCA
SMCHD1-DLGAP1	chr18	2674144	chr18	3742512	9	24	GDFERISNEHSPKLQ	TGACTTTGAAAGAATCTCCAATGAACACTCACCCAAACTCCAGAT

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Information of the samples that have these potential fusion neoantigens of SMCHD1-DLGAP1

These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens.

Cancer type	Fusion gene	Hchr	Hbp	Henst	Tchr	Tbp	Tenst	Sample
STAD	SMCHD1-DLGAP1	chr18	2674144	ENST00000261598	chr18	3742512	ENST00000315677	TCGA-HF-7136-01A

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Potential target of CAR-T therapy development for SMCHD1-DLGAP1

Predicted 3D structure. We used RoseTTAFold.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features

* Minus value of BPloci means that the break point is located before the CDS.

- In-frame and retained 'Transmembrane'.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Subcellular localization prediction of the transmembrane domain retained fusion proteins
* We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image.

Hgene

Hchr

Hbp

Henst

Tgene

Tchr

Tbp

Tenst

DeepLoc result

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Related Drugs to SMCHD1-DLGAP1

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

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Related Diseases to SMCHD1-DLGAP1

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source

	Fusion Gene and Fusion Protein Summary
	Fusion Amino Acid Sequences (multiple BPs and multiple gene isoforms)
	Fusion Protein Breakpoint Sequences - (for the Screening of the FusionNeoAntigens)
	Potential FusionNeoAntigens in HLA I - (netMHCpan v4.1 + deepHLApan v1.1)
	Potential FusionNeoAntigens in HLA II - (netMHCIIpan v4.1)
	Fusion Breakpoint 14 AA Peptide Structure - (RoseTTAFold)
	Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D - (Glide)
	Vaccine Design for the FusionNeoAntigens (RNA/protein sequences)
	Potential target of CAR-T therapy development
	Information on the samples that have these potential fusion neoantigens
	Fusion Protein Targeting Drugs - (Manual Curation)
	Fusion Protein Related diseases - (Manual Curation)