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Center for Computational Systems Medicine
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Fusion Gene and Fusion Protein Summary

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Fusion Amino Acid Sequences (multiple BPs and multiple gene isoforms)

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Fusion Protein Breakpoint Sequences - (for the Screening of the FusionNeoAntigens)

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Potential FusionNeoAntigens in HLA I - (netMHCpan v4.1 + deepHLApan v1.1)

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Potential FusionNeoAntigens in HLA II - (netMHCIIpan v4.1)

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Fusion Breakpoint 14 AA Peptide Structure - (RoseTTAFold)

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D - (Glide)

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Vaccine Design for the FusionNeoAntigens (RNA/protein sequences)

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Potential target of CAR-T therapy development

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Information on the samples that have these potential fusion neoantigens

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Fusion Protein Targeting Drugs - (Manual Curation)

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Fusion Protein Related diseases - (Manual Curation)

Fusion Protein:USF1-SUPT7L

Fusion Gene and Fusion Protein Summary

check button Fusion gene summary
Fusion partner gene informationFusion gene name: USF1-SUPT7L
FusionPDB ID: 97117
FusionGDB2.0 ID: 97117
HgeneTgene
Gene symbol

USF1

SUPT7L

Gene ID

7391

9913

Gene nameupstream transcription factor 1SPT7 like, STAGA complex subunit gamma
SynonymsFCHL|FCHL1|HYPLIP1|MLTF|MLTFI|UEF|bHLHb11SPT7L|STAF65|STAF65(gamma)|STAF65G|SUPT7H
Cytomap

1q23.3

2p23.3

Type of geneprotein-codingprotein-coding
Descriptionupstream stimulatory factor 1class B basic helix-loop-helix protein 11major late transcription factor 1STAGA complex 65 subunit gammaSPT7 like, STAGA complex gamma subunitSPTF-associated factor 65 gammaSTAF65gammaSTAGA complex 65 gamma subunitadenocarcinoma antigen ART1suppressor of Ty 7-like
Modification date2020031320200313
UniProtAcc..
Ensembl transtripts involved in fusion geneENST idsENST00000368019, ENST00000368020, 
ENST00000368021, ENST00000435396, 
ENST00000404798, ENST00000337768, 
ENST00000405491, ENST00000406540, 
ENST00000464789, 
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)* DoF score5 X 8 X 2=803 X 4 X 1=12
# samples 74
** MAII scorelog2(7/80*10)=-0.192645077942396
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
log2(4/12*10)=1.73696559416621
effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs).
DoF>8 and MAII>0
Fusion gene context

PubMed: USF1 [Title/Abstract] AND SUPT7L [Title/Abstract] AND fusion [Title/Abstract]

Fusion neoantigen context

PubMed: USF1 [Title/Abstract] AND SUPT7L [Title/Abstract] AND neoantigen [Title/Abstract]

Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)USF1(161011907)-SUPT7L(27884169), # samples:1
Anticipated loss of major functional domain due to fusion event.USF1-SUPT7L seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
USF1-SUPT7L seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
USF1-SUPT7L seems lost the major protein functional domain in Hgene partner, which is a transcription factor due to the frame-shifted ORF.
USF1-SUPT7L seems lost the major protein functional domain in Tgene partner, which is a epigenetic factor due to the frame-shifted ORF.
USF1-SUPT7L seems lost the major protein functional domain in Tgene partner, which is a essential gene due to the frame-shifted ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgeneUSF1

GO:0006366

transcription by RNA polymerase II

2249772

HgeneUSF1

GO:0019086

late viral transcription

2249772

HgeneUSF1

GO:0032869

cellular response to insulin stimulus

19303849

TgeneSUPT7L

GO:0043966

histone H3 acetylation

11564863

TgeneSUPT7L

GO:0051457

maintenance of protein location in nucleus

15870280



check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr1:161011907/chr2:27884169)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonRetention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here.

check buttonFusion gene breakpoints across USF1 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check buttonFusion gene breakpoints across SUPT7L (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure


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Fusion Amino Acid Sequences


check buttonFusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)
BP loci
(transcript)
Predicted start
(transcript)
Predicted stop
(transcript)
Seq length
(amino acids)
ENST00000368020USF1chr1161011907-ENST00000406540SUPT7Lchr227884169-196939116071966120
ENST00000368020USF1chr1161011907-ENST00000464789SUPT7Lchr227884169-195139115891948120
ENST00000368021USF1chr1161011907-ENST00000337768SUPT7Lchr227884169-429948139374296120
ENST00000368021USF1chr1161011907-ENST00000405491SUPT7Lchr227884169-204848116862045120
ENST00000435396USF1chr1161011907-ENST00000337768SUPT7Lchr227884169-425844038964255120
ENST00000435396USF1chr1161011907-ENST00000405491SUPT7Lchr227884169-200744016452004120
ENST00000368019USF1chr1161011907-ENST00000406540SUPT7Lchr227884169-193836015761935120
ENST00000368019USF1chr1161011907-ENST00000464789SUPT7Lchr227884169-192036015581917120

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000368020ENST00000406540USF1chr1161011907-SUPT7Lchr227884169-0.14270080.85729915
ENST00000368020ENST00000464789USF1chr1161011907-SUPT7Lchr227884169-0.178221270.8217787
ENST00000368021ENST00000337768USF1chr1161011907-SUPT7Lchr227884169-0.064388330.93561167
ENST00000368021ENST00000405491USF1chr1161011907-SUPT7Lchr227884169-0.034919050.9650809
ENST00000435396ENST00000337768USF1chr1161011907-SUPT7Lchr227884169-0.0584093260.9415907
ENST00000435396ENST00000405491USF1chr1161011907-SUPT7Lchr227884169-0.1145534960.88544655
ENST00000368019ENST00000406540USF1chr1161011907-SUPT7Lchr227884169-0.1021731050.8978269
ENST00000368019ENST00000464789USF1chr1161011907-SUPT7Lchr227884169-0.066856050.933144

check button Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones.

Get the fusion protein sequences from here.

Fusion protein sequence information is available in the fasta format.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

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Fusion Protein Breakpoint Sequences for USF1-SUPT7L

check button +/-13 AA sequence from the breakpoints of the fusion protein sequences.
HgeneHchrHbpTgeneTchrTbpLength(fusion protein)BP in fusion proteinPeptide
USF1chr1161011907SUPT7Lchr22788416936014AAPHQGLSQLHATVHDPPLHQPSANK
USF1chr1161011907SUPT7Lchr22788416939114AAPHQGLSQLHATVHDPPLHQPSANK
USF1chr1161011907SUPT7Lchr22788416944014AAPHQGLSQLHATVHDPPLHQPSANK
USF1chr1161011907SUPT7Lchr22788416948114AAPHQGLSQLHATVHDPPLHQPSANK

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Potential FusionNeoAntigen Information of USF1-SUPT7L in HLA I

check button Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.
USF1-SUPT7L_161011907_27884169.msa

check button Potential FusionNeoAntigen Information
* We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5)
Fusion geneHchrHbpTgeneTchrTbpHLA IFusionNeoAntigen peptideBinding scoreImmunogenic scoreNeoantigen start (at BP 13)Neoantigen end (at BP 13)
USF1-SUPT7Lchr1161011907chr227884169360HLA-A02:22GLSQLHATV0.99770.6738514
USF1-SUPT7Lchr1161011907chr227884169360HLA-A02:13GLSQLHATV0.99590.7842514
USF1-SUPT7Lchr1161011907chr227884169360HLA-A02:27GLSQLHATV0.99590.705514
USF1-SUPT7Lchr1161011907chr227884169360HLA-A02:16GLSQLHATV0.99530.6489514
USF1-SUPT7Lchr1161011907chr227884169360HLA-A02:11GLSQLHATV0.99530.6721514
USF1-SUPT7Lchr1161011907chr227884169360HLA-A02:24GLSQLHATV0.99470.6414514
USF1-SUPT7Lchr1161011907chr227884169360HLA-A02:67GLSQLHATV0.99470.6414514
USF1-SUPT7Lchr1161011907chr227884169360HLA-A02:30GLSQLHATV0.99470.6414514
USF1-SUPT7Lchr1161011907chr227884169360HLA-A02:60GLSQLHATV0.99460.6442514
USF1-SUPT7Lchr1161011907chr227884169360HLA-A02:38GLSQLHATV0.99460.8514
USF1-SUPT7Lchr1161011907chr227884169360HLA-A02:04GLSQLHATV0.98920.8881514
USF1-SUPT7Lchr1161011907chr227884169360HLA-A02:19GLSQLHATV0.98170.6405514
USF1-SUPT7Lchr1161011907chr227884169360HLA-A02:17GLSQLHATV0.97120.8587514
USF1-SUPT7Lchr1161011907chr227884169360HLA-B35:03HATVHDPPL0.94650.91441019
USF1-SUPT7Lchr1161011907chr227884169360HLA-A02:35GLSQLHATV0.89390.6535514
USF1-SUPT7Lchr1161011907chr227884169360HLA-A02:29GLSQLHATV0.85330.644514
USF1-SUPT7Lchr1161011907chr227884169360HLA-B35:02HATVHDPPL0.85170.94731019
USF1-SUPT7Lchr1161011907chr227884169360HLA-B35:04HATVHDPPL0.85170.94731019
USF1-SUPT7Lchr1161011907chr227884169360HLA-A02:20GLSQLHATV0.84690.6458514
USF1-SUPT7Lchr1161011907chr227884169360HLA-A66:01TVHDPPLHQ0.6010.76981221
USF1-SUPT7Lchr1161011907chr227884169360HLA-A34:05TVHDPPLHQ0.43310.67091221
USF1-SUPT7Lchr1161011907chr227884169360HLA-A34:01TVHDPPLHQ0.43310.67091221
USF1-SUPT7Lchr1161011907chr227884169360HLA-B13:02GLSQLHATV0.13930.9728514
USF1-SUPT7Lchr1161011907chr227884169360HLA-C03:19HATVHDPPL0.99870.98991019
USF1-SUPT7Lchr1161011907chr227884169360HLA-A02:02GLSQLHATV0.99780.6031514
USF1-SUPT7Lchr1161011907chr227884169360HLA-C03:08HATVHDPPL0.99780.93411019
USF1-SUPT7Lchr1161011907chr227884169360HLA-A02:05GLSQLHATV0.99760.7196514
USF1-SUPT7Lchr1161011907chr227884169360HLA-A02:01GLSQLHATV0.99470.6414514
USF1-SUPT7Lchr1161011907chr227884169360HLA-C08:03HATVHDPPL0.89820.97351019
USF1-SUPT7Lchr1161011907chr227884169360HLA-B35:12HATVHDPPL0.85170.94731019
USF1-SUPT7Lchr1161011907chr227884169360HLA-C03:04HATVHDPPL0.99890.98861019
USF1-SUPT7Lchr1161011907chr227884169360HLA-C03:03HATVHDPPL0.99890.98861019
USF1-SUPT7Lchr1161011907chr227884169360HLA-A02:03GLSQLHATV0.99780.797514
USF1-SUPT7Lchr1161011907chr227884169360HLA-C03:05HATVHDPPL0.99730.94211019
USF1-SUPT7Lchr1161011907chr227884169360HLA-C03:17HATVHDPPL0.99720.96771019
USF1-SUPT7Lchr1161011907chr227884169360HLA-B35:13HATVHDPPL0.94360.92041019
USF1-SUPT7Lchr1161011907chr227884169360HLA-C03:06HATVHDPPL0.93430.98931019
USF1-SUPT7Lchr1161011907chr227884169360HLA-C08:01HATVHDPPL0.89820.97351019
USF1-SUPT7Lchr1161011907chr227884169360HLA-B35:09HATVHDPPL0.85170.94731019
USF1-SUPT7Lchr1161011907chr227884169360HLA-A66:02TVHDPPLHQ0.32820.80231221

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Potential FusionNeoAntigen Information of USF1-SUPT7L in HLA II

check button Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.

check button Potential FusionNeoAntigen Information
* We used NetMHCIIpan v4.1 (%rank<0.5).
Fusion geneHchrHbpTgeneTchrTbpHLA IIFusionNeoAntigen peptideNeoantigen start (at BP 13)Neoantigen end (at BP 13)

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Fusion breakpoint peptide structures of USF1-SUPT7L

check button3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens
* The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA.
File nameBPseqHgeneTgeneHchrHbpTchrTbpAAlen
5657LSQLHATVHDPPLHUSF1SUPT7Lchr1161011907chr227884169360

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of USF1-SUPT7L

check buttonVirtual screening between 25 HLAs (from PDB) and FusionNeoAntigens
* We used Glide to predict the interaction between HLAs and neoantigens.
HLA allelePDB IDFile nameBPseqDocking scoreGlide score
HLA-B14:023BVN5657LSQLHATVHDPPLH-7.15543-7.26883
HLA-B14:023BVN5657LSQLHATVHDPPLH-4.77435-5.80965
HLA-B52:013W395657LSQLHATVHDPPLH-6.80875-6.92215
HLA-B52:013W395657LSQLHATVHDPPLH-4.20386-5.23916
HLA-A11:014UQ25657LSQLHATVHDPPLH-7.5194-8.5547
HLA-A11:014UQ25657LSQLHATVHDPPLH-6.9601-7.0735
HLA-A24:025HGA5657LSQLHATVHDPPLH-7.52403-7.63743
HLA-A24:025HGA5657LSQLHATVHDPPLH-5.82433-6.85963
HLA-B27:056PYJ5657LSQLHATVHDPPLH-3.28285-4.31815
HLA-B44:053DX85657LSQLHATVHDPPLH-5.91172-6.94702
HLA-B44:053DX85657LSQLHATVHDPPLH-4.24346-4.35686

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Vaccine Design for the FusionNeoAntigens of USF1-SUPT7L

check button mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is.
Fusion geneHchrHbpTchrTbpStart in +/-13AAEnd in +/-13AAFusionNeoAntigen peptide sequenceFusionNeoAntigen RNA sequence
USF1-SUPT7Lchr1161011907chr2278841691019HATVHDPPLATGACCCAGGTGCAGAGGTAAATGCTT
USF1-SUPT7Lchr1161011907chr2278841691221TVHDPPLHQCAGGTGCAGAGGTAAATGCTTCTCCTC
USF1-SUPT7Lchr1161011907chr227884169514GLSQLHATVCCTGCCACTCAATCCATGACCCAGGTG

check button mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs.
Fusion geneHchrHbpTchrTbpStart in +/-13AAEnd in +/-13AAFusionNeoAntigen peptideFusionNEoAntigen RNA sequence

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Information of the samples that have these potential fusion neoantigens of USF1-SUPT7L

check button These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens.
Cancer typeFusion geneHchrHbpHenstTchrTbpTenstSample
N/AUSF1-SUPT7Lchr1161011907ENST00000368019chr227884169ENST00000406540BP257468

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Potential target of CAR-T therapy development for USF1-SUPT7L

check button Predicted 3D structure. We used RoseTTAFold.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features


* Minus value of BPloci means that the break point is located before the CDS.
- In-frame and retained 'Transmembrane'.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

check button Subcellular localization prediction of the transmembrane domain retained fusion proteins
* We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image.
HgeneHchrHbpHenstTgeneTchrTbpTenstDeepLoc result

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Related Drugs to USF1-SUPT7L

check button Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDrugSourcePMID

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Related Diseases to USF1-SUPT7L

check button Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDiseaseSourcePMID

check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource