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Center for Computational Systems Medicine
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Fusion Gene and Fusion Protein Summary

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Fusion Amino Acid Sequences (multiple BPs and multiple gene isoforms)

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Fusion Protein Breakpoint Sequences - (for the Screening of the FusionNeoAntigens)

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Potential FusionNeoAntigens in HLA I - (netMHCpan v4.1 + deepHLApan v1.1)

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Potential FusionNeoAntigens in HLA II - (netMHCIIpan v4.1)

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Fusion Breakpoint 14 AA Peptide Structure - (RoseTTAFold)

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D - (Glide)

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Vaccine Design for the FusionNeoAntigens (RNA/protein sequences)

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Potential target of CAR-T therapy development

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Information on the samples that have these potential fusion neoantigens

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Fusion Protein Targeting Drugs - (Manual Curation)

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Fusion Protein Related diseases - (Manual Curation)

Fusion Protein:WNK2-CENPP

Fusion Gene and Fusion Protein Summary

check button Fusion gene summary
Fusion partner gene informationFusion gene name: WNK2-CENPP
FusionPDB ID: 99344
FusionGDB2.0 ID: 99344
HgeneTgene
Gene symbol

WNK2

CENPP

Gene ID

65268

401541

Gene nameWNK lysine deficient protein kinase 2centromere protein P
SynonymsNY-CO-43|P/OKcl.13|PRKWNK2|SDCCAG43CENP-P
Cytomap

9q22.31

9q22.31

Type of geneprotein-codingprotein-coding
Descriptionserine/threonine-protein kinase WNK2antigen NY-CO-43mitogen-activated protein kinase kinase kinaseprotein kinase lysine-deficient 2protein kinase with no lysine 2serologically defined colon cancer antigen 43centromere protein P
Modification date2020031320200313
UniProtAcc.

Q6IPU0

Main function of 5'-partner protein: FUNCTION: Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. {ECO:0000269|PubMed:16622420}.
Ensembl transtripts involved in fusion geneENST idsENST00000356055, ENST00000395475, 
ENST00000349097, ENST00000427277, 
ENST00000297954, ENST00000395477, 
ENST00000471076, 		ENST00000375576, 
ENST00000375579, ENST00000375587, 
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)* DoF score8 X 9 X 8=57612 X 10 X 6=720
# samples 1415
** MAII scorelog2(14/576*10)=-2.04064198449735
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(15/720*10)=-2.26303440583379
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Fusion gene context

PubMed: WNK2 [Title/Abstract] AND CENPP [Title/Abstract] AND fusion [Title/Abstract]

Fusion neoantigen context

PubMed: WNK2 [Title/Abstract] AND CENPP [Title/Abstract] AND neoantigen [Title/Abstract]

Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)WNK2(96070866)-CENPP(95373596), # samples:2
Anticipated loss of major functional domain due to fusion event.WNK2-CENPP seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
WNK2-CENPP seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
WNK2-CENPP seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
WNK2-CENPP seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
WNK2-CENPP seems lost the major protein functional domain in Hgene partner, which is a CGC due to the frame-shifted ORF.
WNK2-CENPP seems lost the major protein functional domain in Hgene partner, which is a IUPHAR drug target due to the frame-shifted ORF.
WNK2-CENPP seems lost the major protein functional domain in Hgene partner, which is a kinase due to the frame-shifted ORF.
WNK2-CENPP seems lost the major protein functional domain in Hgene partner, which is a tumor suppressor due to the frame-shifted ORF.
WNK2-CENPP seems lost the major protein functional domain in Tgene partner, which is a essential gene due to the frame-shifted ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgeneWNK2

GO:0006468

protein phosphorylation

21733846

HgeneWNK2

GO:0046777

protein autophosphorylation

17667937



check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr9:96070866/chr9:95373596)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonRetention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here.

check buttonFusion gene breakpoints across WNK2 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check buttonFusion gene breakpoints across CENPP (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure


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Fusion Amino Acid Sequences


check buttonFusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000297954WNK2chr995947892+ENST00000375587CENPPchr995373596+83686810983327
ENST00000297954WNK2chr995947892+ENST00000375579CENPPchr995373596+29996810983327
ENST00000395475WNK2chr995947892+ENST00000375587CENPPchr995373596+83686810983327
ENST00000395475WNK2chr995947892+ENST00000375579CENPPchr995373596+29996810983327
ENST00000395477WNK2chr995947892+ENST00000375587CENPPchr995373596+83686810983327
ENST00000395477WNK2chr995947892+ENST00000375579CENPPchr995373596+29996810983327
ENST00000427277WNK2chr996052329+ENST00000375576CENPPchr995373596+72534936052371745
ENST00000297954WNK2chr996052329+ENST00000375576CENPPchr995373596+73645047053481782
ENST00000356055WNK2chr996052329+ENST00000375576CENPPchr995373596+72534936052371745
ENST00000349097WNK2chr996052329+ENST00000375576CENPPchr995373596+73645047053481782
ENST00000395477WNK2chr996052329+ENST00000375576CENPPchr995373596+72534936052371745
ENST00000427277WNK2chr996070866+ENST00000375587CENPPchr995373596+142036516068182272
ENST00000427277WNK2chr996070866+ENST00000375579CENPPchr995373596+88346516068182272
ENST00000297954WNK2chr996070866+ENST00000375587CENPPchr995373596+143146627069292309
ENST00000297954WNK2chr996070866+ENST00000375579CENPPchr995373596+89456627069292309
ENST00000349097WNK2chr996070866+ENST00000375587CENPPchr995373596+143146627069292309
ENST00000349097WNK2chr996070866+ENST00000375579CENPPchr995373596+89456627069292309
ENST00000395477WNK2chr996070866+ENST00000375587CENPPchr995373596+142036516068182272
ENST00000395477WNK2chr996070866+ENST00000375579CENPPchr995373596+88346516068182272
ENST00000427277WNK2chr996052329+ENST00000375576CENPPchr995373595+72544936050211673
ENST00000297954WNK2chr996052329+ENST00000375576CENPPchr995373595+73655047051321710
ENST00000356055WNK2chr996052329+ENST00000375576CENPPchr995373595+72544936050211673
ENST00000349097WNK2chr996052329+ENST00000375576CENPPchr995373595+73655047051321710
ENST00000395477WNK2chr996052329+ENST00000375576CENPPchr995373595+72544936050211673

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000297954ENST00000375587WNK2chr995947892+CENPPchr995373596+0.0024495570.9975504
ENST00000297954ENST00000375579WNK2chr995947892+CENPPchr995373596+0.0042950280.99570495
ENST00000395475ENST00000375587WNK2chr995947892+CENPPchr995373596+0.0024495570.9975504
ENST00000395475ENST00000375579WNK2chr995947892+CENPPchr995373596+0.0042950280.99570495
ENST00000395477ENST00000375587WNK2chr995947892+CENPPchr995373596+0.0024495570.9975504
ENST00000395477ENST00000375579WNK2chr995947892+CENPPchr995373596+0.0042950280.99570495
ENST00000427277ENST00000375576WNK2chr996052329+CENPPchr995373596+0.003170180.9968298
ENST00000297954ENST00000375576WNK2chr996052329+CENPPchr995373596+0.0032364250.9967636
ENST00000356055ENST00000375576WNK2chr996052329+CENPPchr995373596+0.003170180.9968298
ENST00000349097ENST00000375576WNK2chr996052329+CENPPchr995373596+0.0032364250.9967636
ENST00000395477ENST00000375576WNK2chr996052329+CENPPchr995373596+0.003170180.9968298
ENST00000427277ENST00000375587WNK2chr996070866+CENPPchr995373596+0.0017801270.9982199
ENST00000427277ENST00000375579WNK2chr996070866+CENPPchr995373596+0.0041435160.99585646
ENST00000297954ENST00000375587WNK2chr996070866+CENPPchr995373596+0.0018310230.99816895
ENST00000297954ENST00000375579WNK2chr996070866+CENPPchr995373596+0.004326590.99567336
ENST00000349097ENST00000375587WNK2chr996070866+CENPPchr995373596+0.0018310230.99816895
ENST00000349097ENST00000375579WNK2chr996070866+CENPPchr995373596+0.004326590.99567336
ENST00000395477ENST00000375587WNK2chr996070866+CENPPchr995373596+0.0017801270.9982199
ENST00000395477ENST00000375579WNK2chr996070866+CENPPchr995373596+0.0041435160.99585646
ENST00000427277ENST00000375576WNK2chr996052329+CENPPchr995373595+0.0032094950.99679047
ENST00000297954ENST00000375576WNK2chr996052329+CENPPchr995373595+0.0032102020.99678975
ENST00000356055ENST00000375576WNK2chr996052329+CENPPchr995373595+0.0032094950.99679047
ENST00000349097ENST00000375576WNK2chr996052329+CENPPchr995373595+0.0032102020.99678975
ENST00000395477ENST00000375576WNK2chr996052329+CENPPchr995373595+0.0032094950.99679047

check button Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones.

Get the fusion protein sequences from here.

Fusion protein sequence information is available in the fasta format.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

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Fusion Protein Breakpoint Sequences for WNK2-CENPP

check button +/-13 AA sequence from the breakpoints of the fusion protein sequences.
HgeneHchrHbpTgeneTchrTbpLength(fusion protein)BP in fusion proteinPeptide
WNK2chr995947892CENPPchr995373596681226DTETWVEVAWCELQEKYPDAVYLSEG
WNK2chr996052329CENPPchr99537359549361640LAPTRGAVMEQGTSSSMTGKVPRCRV
WNK2chr996052329CENPPchr99537359550471677LAPTRGAVMEQGTSSSMTGKVPRCRV
WNK2chr996052329CENPPchr99537359649361638SELAPTRGAVMEQGTSSSMTEKYPDA
WNK2chr996052329CENPPchr99537359650471675SELAPTRGAVMEQGTSSSMTEKYPDA
WNK2chr996070866CENPPchr99537359665162159NQLKQTQKLQDMEAQAGWAAPGEARA
WNK2chr996070866CENPPchr99537359666272196NQLKQTQKLQDMEAQAGWAAPGEARA

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Potential FusionNeoAntigen Information of WNK2-CENPP in HLA I

check button Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.
WNK2-CENPP_95947892_95373596.msa
WNK2-CENPP_96052329_95373595.msa
WNK2-CENPP_96052329_95373596.msa
WNK2-CENPP_96070866_95373596.msa

check button Potential FusionNeoAntigen Information
* We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5)
Fusion geneHchrHbpTgeneTchrTbpHLA IFusionNeoAntigen peptideBinding scoreImmunogenic scoreNeoantigen start (at BP 13)Neoantigen end (at BP 13)
WNK2-CENPPchr995947892chr995373596681HLA-B08:09ELQEKYPDA0.59530.75541120
WNK2-CENPPchr995947892chr995373596681HLA-B15:01LQEKYPDAVY0.99860.79151222
WNK2-CENPPchr995947892chr995373596681HLA-B15:25LQEKYPDAVY0.93550.85671222
WNK2-CENPPchr995947892chr995373596681HLA-B15:03LQEKYPDAVY0.760.71661222
WNK2-CENPPchr995947892chr995373596681HLA-B15:02ELQEKYPDAVY0.99180.84281122
WNK2-CENPPchr995947892chr995373596681HLA-B44:03ELQEKYPDAVY0.97640.87911122
WNK2-CENPPchr995947892chr995373596681HLA-B18:01ELQEKYPDAVY0.89980.78111122
WNK2-CENPPchr995947892chr995373596681HLA-B15:07LQEKYPDAVY0.99610.53681222
WNK2-CENPPchr995947892chr995373596681HLA-B15:04LQEKYPDAVY0.9750.79291222
WNK2-CENPPchr995947892chr995373596681HLA-B15:05LQEKYPDAVY0.86110.7891222
WNK2-CENPPchr995947892chr995373596681HLA-B15:31LQEKYPDAVY0.80390.80141222
WNK2-CENPPchr995947892chr995373596681HLA-B15:31ELQEKYPDAVY0.98880.76721122
WNK2-CENPPchr995947892chr995373596681HLA-B15:125LQEKYPDAVY0.99860.79151222
WNK2-CENPPchr995947892chr995373596681HLA-B15:33LQEKYPDAVY0.99860.79151222
WNK2-CENPPchr995947892chr995373596681HLA-B15:34LQEKYPDAVY0.99860.79151222
WNK2-CENPPchr995947892chr995373596681HLA-B15:27LQEKYPDAVY0.99850.82121222
WNK2-CENPPchr995947892chr995373596681HLA-B15:135LQEKYPDAVY0.99830.78751222
WNK2-CENPPchr995947892chr995373596681HLA-B15:50LQEKYPDAVY0.99640.90091222
WNK2-CENPPchr995947892chr995373596681HLA-B15:24LQEKYPDAVY0.9950.84681222
WNK2-CENPPchr995947892chr995373596681HLA-B15:35LQEKYPDAVY0.99410.75521222
WNK2-CENPPchr995947892chr995373596681HLA-B15:12LQEKYPDAVY0.98740.77611222
WNK2-CENPPchr995947892chr995373596681HLA-B15:53LQEKYPDAVY0.98740.75691222
WNK2-CENPPchr995947892chr995373596681HLA-B15:54LQEKYPDAVY0.98510.74121222
WNK2-CENPPchr995947892chr995373596681HLA-B15:39LQEKYPDAVY0.94320.77581222
WNK2-CENPPchr995947892chr995373596681HLA-B15:20LQEKYPDAVY0.86230.83941222
WNK2-CENPPchr995947892chr995373596681HLA-B35:28LQEKYPDAVY0.84160.83851222
WNK2-CENPPchr995947892chr995373596681HLA-B35:20LQEKYPDAVY0.77490.8471222
WNK2-CENPPchr995947892chr995373596681HLA-B18:11LQEKYPDAVY0.68260.89191222
WNK2-CENPPchr995947892chr995373596681HLA-B48:02LQEKYPDAVY0.6790.8181222
WNK2-CENPPchr995947892chr995373596681HLA-A25:01EVAWCELQEKY0.99920.7921617
WNK2-CENPPchr995947892chr995373596681HLA-B44:13ELQEKYPDAVY0.97640.87911122
WNK2-CENPPchr995947892chr995373596681HLA-B44:26ELQEKYPDAVY0.97640.87911122
WNK2-CENPPchr995947892chr995373596681HLA-B44:07ELQEKYPDAVY0.97640.87911122
WNK2-CENPPchr995947892chr995373596681HLA-B18:11ELQEKYPDAVY0.92030.75631122
WNK2-CENPPchr995947892chr995373596681HLA-B18:05ELQEKYPDAVY0.89980.78111122
WNK2-CENPPchr996052329chr9953735955047HLA-A30:08GTSSSMTGK0.95390.75981120
WNK2-CENPPchr996052329chr9953735955047HLA-A30:01GTSSSMTGK0.95090.87371120
WNK2-CENPPchr996052329chr9953735965047HLA-B44:03MEQGTSSSM0.98450.98811019
WNK2-CENPPchr996052329chr9953735965047HLA-B47:01MEQGTSSSM0.97080.76321019
WNK2-CENPPchr996052329chr9953735965047HLA-B18:01MEQGTSSSM0.96120.95771019
WNK2-CENPPchr996052329chr9953735965047HLA-B44:05MEQGTSSSM0.96120.60181019
WNK2-CENPPchr996052329chr9953735965047HLA-B50:02MEQGTSSSM0.91970.76311019
WNK2-CENPPchr996052329chr9953735965047HLA-B15:10MEQGTSSSM0.8750.81019
WNK2-CENPPchr996052329chr9953735965047HLA-B39:13MEQGTSSSM0.82980.98161019
WNK2-CENPPchr996052329chr9953735965047HLA-B15:37MEQGTSSSM0.82120.81111019
WNK2-CENPPchr996052329chr9953735965047HLA-B15:18MEQGTSSSM0.80950.92511019
WNK2-CENPPchr996052329chr9953735965047HLA-B15:03MEQGTSSSM0.75370.94871019
WNK2-CENPPchr996052329chr9953735965047HLA-B50:01MEQGTSSSM0.63410.87451019
WNK2-CENPPchr996052329chr9953735965047HLA-B41:01MEQGTSSSM0.46020.98661019
WNK2-CENPPchr996052329chr9953735965047HLA-B40:01AVMEQGTSSSM0.42650.8156819
WNK2-CENPPchr996052329chr9953735965047HLA-B48:03MEQGTSSSM0.99440.55431019
WNK2-CENPPchr996052329chr9953735965047HLA-B44:09MEQGTSSSM0.98480.61661019
WNK2-CENPPchr996052329chr9953735965047HLA-B44:08MEQGTSSSM0.98160.52611019
WNK2-CENPPchr996052329chr9953735965047HLA-B40:03MEQGTSSSM0.98040.62161019
WNK2-CENPPchr996052329chr9953735965047HLA-B44:10MEQGTSSSM0.9760.64851019
WNK2-CENPPchr996052329chr9953735965047HLA-B39:05MEQGTSSSM0.840.97461019
WNK2-CENPPchr996052329chr9953735965047HLA-B39:08MEQGTSSSM0.83820.93571019
WNK2-CENPPchr996052329chr9953735965047HLA-B15:31MEQGTSSSM0.68380.97981019
WNK2-CENPPchr996052329chr9953735965047HLA-B44:09AVMEQGTSSSM0.63660.8844819
WNK2-CENPPchr996052329chr9953735965047HLA-B40:04MEQGTSSSM0.99730.80371019
WNK2-CENPPchr996052329chr9953735965047HLA-B40:12MEQGTSSSM0.99440.55431019
WNK2-CENPPchr996052329chr9953735965047HLA-B44:13MEQGTSSSM0.98450.98811019
WNK2-CENPPchr996052329chr9953735965047HLA-B44:26MEQGTSSSM0.98450.98811019
WNK2-CENPPchr996052329chr9953735965047HLA-B44:07MEQGTSSSM0.98450.98811019
WNK2-CENPPchr996052329chr9953735965047HLA-B18:04MEQGTSSSM0.97370.96361019
WNK2-CENPPchr996052329chr9953735965047HLA-B18:07MEQGTSSSM0.96660.93621019
WNK2-CENPPchr996052329chr9953735965047HLA-B18:05MEQGTSSSM0.96120.95771019
WNK2-CENPPchr996052329chr9953735965047HLA-B18:06MEQGTSSSM0.95980.96361019
WNK2-CENPPchr996052329chr9953735965047HLA-B18:08MEQGTSSSM0.95780.95561019
WNK2-CENPPchr996052329chr9953735965047HLA-B18:11MEQGTSSSM0.92110.96481019
WNK2-CENPPchr996052329chr9953735965047HLA-B18:03MEQGTSSSM0.90680.95421019
WNK2-CENPPchr996052329chr9953735965047HLA-B39:02MEQGTSSSM0.84490.9821019
WNK2-CENPPchr996052329chr9953735965047HLA-B39:31MEQGTSSSM0.840.97731019
WNK2-CENPPchr996052329chr9953735965047HLA-B39:11MEQGTSSSM0.81610.94011019
WNK2-CENPPchr996052329chr9953735965047HLA-B48:02MEQGTSSSM0.75260.98361019
WNK2-CENPPchr996052329chr9953735965047HLA-B35:20MEQGTSSSM0.670.98671019
WNK2-CENPPchr996052329chr9953735965047HLA-B35:28MEQGTSSSM0.66350.98481019
WNK2-CENPPchr996052329chr9953735965047HLA-B41:03MEQGTSSSM0.65210.76341019
WNK2-CENPPchr996052329chr9953735965047HLA-B50:05MEQGTSSSM0.63410.87451019
WNK2-CENPPchr996052329chr9953735965047HLA-B50:04MEQGTSSSM0.63410.87451019
WNK2-CENPPchr996052329chr9953735965047HLA-B15:09MEQGTSSSM0.63290.94951019
WNK2-CENPPchr996052329chr9953735965047HLA-B15:53MEQGTSSSM0.5610.95571019
WNK2-CENPPchr996052329chr9953735965047HLA-B15:54MEQGTSSSM0.54820.94851019
WNK2-CENPPchr996052329chr9953735965047HLA-B15:30AVMEQGTSSSM0.99320.9865819
WNK2-CENPPchr996052329chr9953735965047HLA-B41:03AVMEQGTSSSM0.73680.7744819
WNK2-CENPPchr996052329chr9953735965047HLA-B40:04AVMEQGTSSSM0.50340.8259819
WNK2-CENPPchr996070866chr9953735966627HLA-B45:01MEAQAGWAA0.99650.96451120
WNK2-CENPPchr996070866chr9953735966627HLA-B50:02MEAQAGWAA0.99350.80361120
WNK2-CENPPchr996070866chr9953735966627HLA-A02:22KLQDMEAQA0.98850.865716
WNK2-CENPPchr996070866chr9953735966627HLA-B44:03QDMEAQAGW0.98770.9651918
WNK2-CENPPchr996070866chr9953735966627HLA-A02:60KLQDMEAQA0.98420.8536716
WNK2-CENPPchr996070866chr9953735966627HLA-A02:24KLQDMEAQA0.98370.8619716
WNK2-CENPPchr996070866chr9953735966627HLA-A02:67KLQDMEAQA0.98370.8619716
WNK2-CENPPchr996070866chr9953735966627HLA-A02:30KLQDMEAQA0.98370.8619716
WNK2-CENPPchr996070866chr9953735966627HLA-A02:11KLQDMEAQA0.98330.8732716
WNK2-CENPPchr996070866chr9953735966627HLA-A02:16KLQDMEAQA0.97650.8577716
WNK2-CENPPchr996070866chr9953735966627HLA-B44:02QDMEAQAGW0.9710.7743918
WNK2-CENPPchr996070866chr9953735966627HLA-A02:27KLQDMEAQA0.96960.8632716
WNK2-CENPPchr996070866chr9953735966627HLA-A02:38KLQDMEAQA0.96880.8449716
WNK2-CENPPchr996070866chr9953735966627HLA-A02:13KLQDMEAQA0.96540.91716
WNK2-CENPPchr996070866chr9953735966627HLA-B44:05QDMEAQAGW0.93840.884918
WNK2-CENPPchr996070866chr9953735966627HLA-A02:19KLQDMEAQA0.80050.7965716
WNK2-CENPPchr996070866chr9953735966627HLA-A02:29KLQDMEAQA0.79780.8643716
WNK2-CENPPchr996070866chr9953735966627HLA-A02:20KLQDMEAQA0.78470.8672716
WNK2-CENPPchr996070866chr9953735966627HLA-B18:01MEAQAGWAA0.78040.93141120
WNK2-CENPPchr996070866chr9953735966627HLA-B50:01MEAQAGWAA0.65560.95861120
WNK2-CENPPchr996070866chr9953735966627HLA-B41:01MEAQAGWAA0.60120.98821120
WNK2-CENPPchr996070866chr9953735966627HLA-B45:01MEAQAGWAAP0.99430.94521121
WNK2-CENPPchr996070866chr9953735966627HLA-B50:02MEAQAGWAAP0.9870.78361121
WNK2-CENPPchr996070866chr9953735966627HLA-A32:13KLQDMEAQAGW0.99270.9956718
WNK2-CENPPchr996070866chr9953735966627HLA-B45:01QDMEAQAGWAA0.9860.9984920
WNK2-CENPPchr996070866chr9953735966627HLA-B40:06MEAQAGWAA0.99790.97211120
WNK2-CENPPchr996070866chr9953735966627HLA-A02:02KLQDMEAQA0.98840.8208716
WNK2-CENPPchr996070866chr9953735966627HLA-A02:01KLQDMEAQA0.98370.8619716
WNK2-CENPPchr996070866chr9953735966627HLA-B44:04QDMEAQAGW0.97620.5444918
WNK2-CENPPchr996070866chr9953735966627HLA-B44:08QDMEAQAGW0.96910.8333918
WNK2-CENPPchr996070866chr9953735966627HLA-B44:06QDMEAQAGW0.96170.5621918
WNK2-CENPPchr996070866chr9953735966627HLA-B44:10QDMEAQAGW0.33130.9564918
WNK2-CENPPchr996070866chr9953735966627HLA-B44:08LQDMEAQAGW0.86610.9508818
WNK2-CENPPchr996070866chr9953735966627HLA-B44:07QDMEAQAGW0.98770.9651918
WNK2-CENPPchr996070866chr9953735966627HLA-B44:26QDMEAQAGW0.98770.9651918
WNK2-CENPPchr996070866chr9953735966627HLA-B44:13QDMEAQAGW0.98770.9651918
WNK2-CENPPchr996070866chr9953735966627HLA-B40:04MEAQAGWAA0.98280.95111120
WNK2-CENPPchr996070866chr9953735966627HLA-B44:22QDMEAQAGW0.9710.7743918
WNK2-CENPPchr996070866chr9953735966627HLA-B44:21QDMEAQAGW0.9140.5668918
WNK2-CENPPchr996070866chr9953735966627HLA-B18:05MEAQAGWAA0.78040.93141120
WNK2-CENPPchr996070866chr9953735966627HLA-B18:06MEAQAGWAA0.73930.94771120
WNK2-CENPPchr996070866chr9953735966627HLA-B18:03MEAQAGWAA0.71730.92521120
WNK2-CENPPchr996070866chr9953735966627HLA-B50:05MEAQAGWAA0.65560.95861120
WNK2-CENPPchr996070866chr9953735966627HLA-B50:04MEAQAGWAA0.65560.95861120
WNK2-CENPPchr996070866chr9953735966627HLA-B18:11MEAQAGWAA0.54550.93981120
WNK2-CENPPchr996070866chr9953735966627HLA-B15:24LQDMEAQAGW0.89530.9897818
WNK2-CENPPchr996070866chr9953735966627HLA-A32:01KLQDMEAQAGW0.99830.9968718

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Potential FusionNeoAntigen Information of WNK2-CENPP in HLA II

check button Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.
WNK2-CENPP_96070866_95373596.msa

check button Potential FusionNeoAntigen Information
* We used NetMHCIIpan v4.1 (%rank<0.5).
Fusion geneHchrHbpTgeneTchrTbpHLA IIFusionNeoAntigen peptideNeoantigen start (at BP 13)Neoantigen end (at BP 13)
WNK2-CENPPchr996070866chr9953735966627DRB1-0806NQLKQTQKLQDMEAQ015
WNK2-CENPPchr996070866chr9953735966627DRB1-0822NQLKQTQKLQDMEAQ015
WNK2-CENPPchr996070866chr9953735966627DRB1-0829NQLKQTQKLQDMEAQ015
WNK2-CENPPchr996070866chr9953735966627DRB1-1002TQKLQDMEAQAGWAA520
WNK2-CENPPchr996070866chr9953735966627DRB1-1002QTQKLQDMEAQAGWA419
WNK2-CENPPchr996070866chr9953735966627DRB1-1303NQLKQTQKLQDMEAQ015
WNK2-CENPPchr996070866chr9953735966627DRB1-1312NQLKQTQKLQDMEAQ015
WNK2-CENPPchr996070866chr9953735966627DRB1-1358NQLKQTQKLQDMEAQ015
WNK2-CENPPchr996070866chr9953735966627DRB1-1381NQLKQTQKLQDMEAQ015
WNK2-CENPPchr996070866chr9953735966627DRB1-1388NQLKQTQKLQDMEAQ015
WNK2-CENPPchr996070866chr9953735966627DRB1-1389NQLKQTQKLQDMEAQ015
WNK2-CENPPchr996070866chr9953735966627DRB1-1390NQLKQTQKLQDMEAQ015
WNK2-CENPPchr996070866chr9953735966627DRB1-1395NQLKQTQKLQDMEAQ015

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Fusion breakpoint peptide structures of WNK2-CENPP

check button3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens
* The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA.
File nameBPseqHgeneTgeneHchrHbpTchrTbpAAlen
698AVMEQGTSSSMTGKWNK2CENPPchr996052329chr9953735955047
File nameBPseqHgeneTgeneHchrHbpTchrTbpAAlen
2199EVAWCELQEKYPDAWNK2CENPPchr995947892chr995373596681
File nameBPseqHgeneTgeneHchrHbpTchrTbpAAlen
7352QKLQDMEAQAGWAAWNK2CENPPchr996070866chr9953735966627
File nameBPseqHgeneTgeneHchrHbpTchrTbpAAlen
7850RGAVMEQGTSSSMTWNK2CENPPchr996052329chr9953735965047

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of WNK2-CENPP

check buttonVirtual screening between 25 HLAs (from PDB) and FusionNeoAntigens
* We used Glide to predict the interaction between HLAs and neoantigens.
HLA allelePDB IDFile nameBPseqDocking scoreGlide score
HLA-B14:023BVN698AVMEQGTSSSMTGK-7.63753-7.75093
HLA-B14:023BVN698AVMEQGTSSSMTGK-4.36349-5.39879
HLA-B52:013W39698AVMEQGTSSSMTGK-6.74822-6.86162
HLA-B52:013W39698AVMEQGTSSSMTGK-4.96916-6.00446
HLA-A24:025HGA698AVMEQGTSSSMTGK-8.26666-9.30196
HLA-A24:025HGA698AVMEQGTSSSMTGK-7.6208-7.7342
HLA-B27:036PZ5698AVMEQGTSSSMTGK0.0420189-0.993281
HLA-B44:053DX8698AVMEQGTSSSMTGK-5.06122-5.17462
HLA-B44:053DX8698AVMEQGTSSSMTGK-4.87073-5.90603
HLA-B14:023BVN2199EVAWCELQEKYPDA-7.9962-8.1096
HLA-B14:023BVN2199EVAWCELQEKYPDA-5.70842-6.74372
HLA-B52:013W392199EVAWCELQEKYPDA-6.83737-6.95077
HLA-B52:013W392199EVAWCELQEKYPDA-4.4836-5.5189
HLA-A11:014UQ22199EVAWCELQEKYPDA-10.0067-10.1201
HLA-A11:014UQ22199EVAWCELQEKYPDA-9.03915-10.0745
HLA-A24:025HGA2199EVAWCELQEKYPDA-6.56204-6.67544
HLA-A24:025HGA2199EVAWCELQEKYPDA-5.42271-6.45801
HLA-B44:053DX82199EVAWCELQEKYPDA-7.85648-8.89178
HLA-B44:053DX82199EVAWCELQEKYPDA-5.3978-5.5112
HLA-A02:016TDR2199EVAWCELQEKYPDA-3.37154-4.40684
HLA-B14:023BVN7352QKLQDMEAQAGWAA-7.21412-7.40612
HLA-B14:023BVN7352QKLQDMEAQAGWAA-6.03453-6.79553
HLA-B52:013W397352QKLQDMEAQAGWAA-6.42933-6.62133
HLA-B52:013W397352QKLQDMEAQAGWAA-3.99008-4.75108
HLA-A11:014UQ27352QKLQDMEAQAGWAA-4.97642-5.73742
HLA-A24:025HGA7352QKLQDMEAQAGWAA-6.79795-6.98995
HLA-A24:025HGA7352QKLQDMEAQAGWAA-4.69916-5.46016
HLA-B27:036PZ57352QKLQDMEAQAGWAA-0.33821-1.09921
HLA-B44:053DX87352QKLQDMEAQAGWAA-5.01856-5.21056
HLA-B44:053DX87352QKLQDMEAQAGWAA-3.77281-4.53381
HLA-B14:023BVN7850RGAVMEQGTSSSMT-7.9962-8.1096
HLA-B14:023BVN7850RGAVMEQGTSSSMT-5.70842-6.74372
HLA-B52:013W397850RGAVMEQGTSSSMT-6.83737-6.95077
HLA-B52:013W397850RGAVMEQGTSSSMT-4.4836-5.5189
HLA-A11:014UQ27850RGAVMEQGTSSSMT-10.0067-10.1201
HLA-A11:014UQ27850RGAVMEQGTSSSMT-9.03915-10.0745
HLA-A24:025HGA7850RGAVMEQGTSSSMT-6.56204-6.67544
HLA-A24:025HGA7850RGAVMEQGTSSSMT-5.42271-6.45801
HLA-B44:053DX87850RGAVMEQGTSSSMT-7.85648-8.89178
HLA-B44:053DX87850RGAVMEQGTSSSMT-5.3978-5.5112
HLA-A02:016TDR7850RGAVMEQGTSSSMT-3.37154-4.40684

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Vaccine Design for the FusionNeoAntigens of WNK2-CENPP

check button mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is.
Fusion geneHchrHbpTchrTbpStart in +/-13AAEnd in +/-13AAFusionNeoAntigen peptide sequenceFusionNeoAntigen RNA sequence
WNK2-CENPPchr995947892chr9953735961120ELQEKYPDACTGCAGGAAAAGTACCCAGATGCCGTG
WNK2-CENPPchr995947892chr9953735961122ELQEKYPDAVYCTGCAGGAAAAGTACCCAGATGCCGTGTACCTC
WNK2-CENPPchr995947892chr9953735961222LQEKYPDAVYCAGGAAAAGTACCCAGATGCCGTGTACCTC
WNK2-CENPPchr995947892chr995373596617EVAWCELQEKYGTGGCCTGGTGTGAGCTGCAGGAAAAGTACCCA
WNK2-CENPPchr996052329chr9953735951120GTSSSMTGKTGACAGGAAAAGTACCCAGATGCCGTG
WNK2-CENPPchr996052329chr9953735961019MEQGTSSSMCAATGACAGAAAAGTACCCAGATGCCG
WNK2-CENPPchr996052329chr995373596819AVMEQGTSSSMCCTCGTCAATGACAGAAAAGTACCCAGATGCCG
WNK2-CENPPchr996070866chr9953735961120MEAQAGWAACGGGCTGAAAAGTACCCAGATGCCGTG
WNK2-CENPPchr996070866chr9953735961121MEAQAGWAAPCGGGCTGAAAAGTACCCAGATGCCGTGTAC
WNK2-CENPPchr996070866chr995373596716KLQDMEAQACCTGGCGAGGCGCGGGCTGAAAAGTAC
WNK2-CENPPchr996070866chr995373596718KLQDMEAQAGWCCTGGCGAGGCGCGGGCTGAAAAGTACCCAGAT
WNK2-CENPPchr996070866chr995373596818LQDMEAQAGWGGCGAGGCGCGGGCTGAAAAGTACCCAGAT
WNK2-CENPPchr996070866chr995373596918QDMEAQAGWGAGGCGCGGGCTGAAAAGTACCCAGAT
WNK2-CENPPchr996070866chr995373596920QDMEAQAGWAAGAGGCGCGGGCTGAAAAGTACCCAGATGCCGTG

check button mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs.
Fusion geneHchrHbpTchrTbpStart in +/-13AAEnd in +/-13AAFusionNeoAntigen peptideFusionNEoAntigen RNA sequence
WNK2-CENPPchr996070866chr995373596015NQLKQTQKLQDMEAQGCCCAGGCAGGCTGGGCTGCCCCTGGCGAGGCGCGGGCTGAAAAG
WNK2-CENPPchr996070866chr995373596419QTQKLQDMEAQAGWATGGGCTGCCCCTGGCGAGGCGCGGGCTGAAAAGTACCCAGATGCC
WNK2-CENPPchr996070866chr995373596520TQKLQDMEAQAGWAAGCTGCCCCTGGCGAGGCGCGGGCTGAAAAGTACCCAGATGCCGTG

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Information of the samples that have these potential fusion neoantigens of WNK2-CENPP

check button These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens.
Cancer typeFusion geneHchrHbpHenstTchrTbpTenstSample
LGGWNK2-CENPPchr996052329ENST00000297954chr995373596ENST00000375576TCGA-TQ-A7RR-01A
LGGWNK2-CENPPchr996052329ENST00000297954chr995373595ENST00000375576TCGA-TQ-A7RR
BLCAWNK2-CENPPchr995947892ENST00000297954chr995373596ENST00000375579TCGA-BL-A0C8-01A
UCSWNK2-CENPPchr996070866ENST00000297954chr995373596ENST00000375579TCGA-NF-A4WU-01A

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Potential target of CAR-T therapy development for WNK2-CENPP

check button Predicted 3D structure. We used RoseTTAFold.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features


* Minus value of BPloci means that the break point is located before the CDS.
- In-frame and retained 'Transmembrane'.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

check button Subcellular localization prediction of the transmembrane domain retained fusion proteins
* We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image.
HgeneHchrHbpHenstTgeneTchrTbpTenstDeepLoc result

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Related Drugs to WNK2-CENPP

check button Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDrugSourcePMID

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Related Diseases to WNK2-CENPP

check button Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDiseaseSourcePMID

check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource