FusionNeoAntigen Logo

Home

Download

Statistics

Examples

Help

Contact

Terms of Use
Center for Computational Systems Medicine
leaf

Fusion Gene and Fusion Protein Summary

leaf

Fusion Amino Acid Sequences (multiple BPs and multiple gene isoforms)

leaf

Fusion Protein Breakpoint Sequences - (for the Screening of the FusionNeoAntigens)

leaf

Potential FusionNeoAntigens in HLA I - (netMHCpan v4.1 + deepHLApan v1.1)

leaf

Potential FusionNeoAntigens in HLA II - (netMHCIIpan v4.1)

leaf

Fusion Breakpoint 14 AA Peptide Structure - (RoseTTAFold)

leaf

Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D - (Glide)

leaf

Vaccine Design for the FusionNeoAntigens (RNA/protein sequences)

leaf

Potential target of CAR-T therapy development

leaf

Information on the samples that have these potential fusion neoantigens

leaf

Fusion Protein Targeting Drugs - (Manual Curation)

leaf

Fusion Protein Related diseases - (Manual Curation)

Fusion Protein:WWOX-C15orf41

Fusion Gene and Fusion Protein Summary

check button Fusion gene summary
Fusion partner gene informationFusion gene name: WWOX-C15orf41
FusionPDB ID: 99475
FusionGDB2.0 ID: 99475
HgeneTgene
Gene symbol

WWOX

C15orf41

Gene ID

51741

84529

Gene nameWW domain containing oxidoreductasechromosome 15 open reading frame 41
SynonymsD16S432E|EIEE28|FOR|FRA16D|HHCMA56|PRO0128|SCAR12|SDR41C1|WOX1HH114
Cytomap

16q23.1-q23.2

15q14

Type of geneprotein-codingprotein-coding
DescriptionWW domain-containing oxidoreductaseWW domain-containing protein WWOXfragile site FRA16D oxidoreductaseshort chain dehydrogenase/reductase family 41C member 1protein C15orf41uncharacterized protein C15orf41
Modification date2020031320200313
UniProtAcc..
Ensembl transtripts involved in fusion geneENST idsENST00000565791, ENST00000355860, 
ENST00000406884, ENST00000408984, 
ENST00000566780, ENST00000402655, 
ENST00000539474, ENST00000569818, 
ENST00000338183, ENST00000437989, 
ENST00000562877, ENST00000566621, 
ENST00000567389, ENST00000569302, 
ENST00000562489, ENST00000563167, 
ENST00000565792, 
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)* DoF score26 X 26 X 8=54088 X 5 X 7=280
# samples 3110
** MAII scorelog2(31/5408*10)=-4.12475503100795
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(10/280*10)=-1.48542682717024
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Fusion gene context

PubMed: WWOX [Title/Abstract] AND C15orf41 [Title/Abstract] AND fusion [Title/Abstract]

Fusion neoantigen context

PubMed: WWOX [Title/Abstract] AND C15orf41 [Title/Abstract] AND neoantigen [Title/Abstract]

Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)WWOX(78198186)-C15orf41(37100525), # samples:2
Anticipated loss of major functional domain due to fusion event.WWOX-C15orf41 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
WWOX-C15orf41 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
WWOX-C15orf41 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
WWOX-C15orf41 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
WWOX-C15orf41 seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF.
WWOX-C15orf41 seems lost the major protein functional domain in Hgene partner, which is a tumor suppressor due to the frame-shifted ORF.
WWOX-C15orf41 seems lost the major protein functional domain in Tgene partner, which is a essential gene due to the frame-shifted ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgeneWWOX

GO:0030178

negative regulation of Wnt signaling pathway

19465938

HgeneWWOX

GO:0071560

cellular response to transforming growth factor beta stimulus

19366691



check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr16:78198186/chr15:37100525)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonRetention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here.

check buttonFusion gene breakpoints across WWOX (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check buttonFusion gene breakpoints across C15orf41 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure


Top

Fusion Amino Acid Sequences


check buttonFusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000566780WWOXchr1678198186+ENST00000566621C15orf41chr1537100525+27828823661118250
ENST00000566780WWOXchr1678198186+ENST00000437989C15orf41chr1537100525+23898823661079237
ENST00000566780WWOXchr1678198186+ENST00000562877C15orf41chr1537100525+10128823661010215
ENST00000406884WWOXchr1678198186+ENST00000566621C15orf41chr1537100525+2541641125877250
ENST00000406884WWOXchr1678198186+ENST00000437989C15orf41chr1537100525+2148641125838237
ENST00000406884WWOXchr1678198186+ENST00000562877C15orf41chr1537100525+771641125769215
ENST00000408984WWOXchr1678198186+ENST00000566621C15orf41chr1537100525+250160185837250
ENST00000408984WWOXchr1678198186+ENST00000437989C15orf41chr1537100525+210860185798237
ENST00000408984WWOXchr1678198186+ENST00000562877C15orf41chr1537100525+73160185729215
ENST00000355860WWOXchr1678198186+ENST00000566621C15orf41chr1537100525+2516616100852250
ENST00000355860WWOXchr1678198186+ENST00000437989C15orf41chr1537100525+2123616100813237
ENST00000355860WWOXchr1678198186+ENST00000562877C15orf41chr1537100525+746616100744215

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000566780ENST00000566621WWOXchr1678198186+C15orf41chr1537100525+0.0041141920.9958858
ENST00000566780ENST00000437989WWOXchr1678198186+C15orf41chr1537100525+0.0028946060.9971054
ENST00000566780ENST00000562877WWOXchr1678198186+C15orf41chr1537100525+0.79791240.20208761
ENST00000406884ENST00000566621WWOXchr1678198186+C15orf41chr1537100525+0.002905540.9970945
ENST00000406884ENST00000437989WWOXchr1678198186+C15orf41chr1537100525+0.0023478370.99765223
ENST00000406884ENST00000562877WWOXchr1678198186+C15orf41chr1537100525+0.85343780.1465622
ENST00000408984ENST00000566621WWOXchr1678198186+C15orf41chr1537100525+0.0024934180.99750656
ENST00000408984ENST00000437989WWOXchr1678198186+C15orf41chr1537100525+0.0018569530.9981431
ENST00000408984ENST00000562877WWOXchr1678198186+C15orf41chr1537100525+0.73189750.26810247
ENST00000355860ENST00000566621WWOXchr1678198186+C15orf41chr1537100525+0.0027050260.99729496
ENST00000355860ENST00000437989WWOXchr1678198186+C15orf41chr1537100525+0.0018386610.9981614
ENST00000355860ENST00000562877WWOXchr1678198186+C15orf41chr1537100525+0.7885920.211408

check button Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones.

Get the fusion protein sequences from here.

Fusion protein sequence information is available in the fasta format.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

Top

Fusion Protein Breakpoint Sequences for WWOX-C15orf41

check button +/-13 AA sequence from the breakpoints of the fusion protein sequences.
HgeneHchrHbpTgeneTchrTbpLength(fusion protein)BP in fusion proteinPeptide
WWOXchr1678198186C15orf41chr1537100525601159HGAHVILACRNMARASEAVSRILEEW
WWOXchr1678198186C15orf41chr1537100525616159HGAHVILACRNMARASEAVSRILEEW
WWOXchr1678198186C15orf41chr1537100525641159HGAHVILACRNMARASEAVSRILEEW
WWOXchr1678198186C15orf41chr1537100525882159HGAHVILACRNMARASEAVSRILEEW

Top

Potential FusionNeoAntigen Information of WWOX-C15orf41 in HLA I

check button Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.
WWOX-C15orf41_78198186_37100525.msa

check button Potential FusionNeoAntigen Information
* We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5)
Fusion geneHchrHbpTgeneTchrTbpHLA IFusionNeoAntigen peptideBinding scoreImmunogenic scoreNeoantigen start (at BP 13)Neoantigen end (at BP 13)
WWOX-C15orf41chr1678198186chr1537100525616HLA-B27:05ARASEAVSR0.99790.56071221
WWOX-C15orf41chr1678198186chr1537100525616HLA-A02:19NMARASEAV0.7510.59511019
WWOX-C15orf41chr1678198186chr1537100525616HLA-B27:14ARASEAVSR0.99760.59981221
WWOX-C15orf41chr1678198186chr1537100525616HLA-B27:03ARASEAVSR0.92140.60231221
WWOX-C15orf41chr1678198186chr1537100525616HLA-B27:10ARASEAVSR0.99650.71391221
WWOX-C15orf41chr1678198186chr1537100525616HLA-B27:06ARASEAVSRIL0.99990.65991223
WWOX-C15orf41chr1678198186chr1537100525616HLA-B27:09ARASEAVSRIL0.99970.70851223

Top

Potential FusionNeoAntigen Information of WWOX-C15orf41 in HLA II

check button Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.

check button Potential FusionNeoAntigen Information
* We used NetMHCIIpan v4.1 (%rank<0.5).
Fusion geneHchrHbpTgeneTchrTbpHLA IIFusionNeoAntigen peptideNeoantigen start (at BP 13)Neoantigen end (at BP 13)

Top

Fusion breakpoint peptide structures of WWOX-C15orf41

check button3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens
* The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA.
File nameBPseqHgeneTgeneHchrHbpTchrTbpAAlen
4729LACRNMARASEAVSWWOXC15orf41chr1678198186chr1537100525616

Top

Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of WWOX-C15orf41

check buttonVirtual screening between 25 HLAs (from PDB) and FusionNeoAntigens
* We used Glide to predict the interaction between HLAs and neoantigens.
HLA allelePDB IDFile nameBPseqDocking scoreGlide score
HLA-B14:023BVN4729LACRNMARASEAVS-7.9962-8.1096
HLA-B14:023BVN4729LACRNMARASEAVS-5.70842-6.74372
HLA-B52:013W394729LACRNMARASEAVS-6.83737-6.95077
HLA-B52:013W394729LACRNMARASEAVS-4.4836-5.5189
HLA-A11:014UQ24729LACRNMARASEAVS-10.0067-10.1201
HLA-A11:014UQ24729LACRNMARASEAVS-9.03915-10.0745
HLA-A24:025HGA4729LACRNMARASEAVS-6.56204-6.67544
HLA-A24:025HGA4729LACRNMARASEAVS-5.42271-6.45801
HLA-B44:053DX84729LACRNMARASEAVS-7.85648-8.89178
HLA-B44:053DX84729LACRNMARASEAVS-5.3978-5.5112
HLA-A02:016TDR4729LACRNMARASEAVS-3.37154-4.40684

Top

Vaccine Design for the FusionNeoAntigens of WWOX-C15orf41

check button mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is.
Fusion geneHchrHbpTchrTbpStart in +/-13AAEnd in +/-13AAFusionNeoAntigen peptide sequenceFusionNeoAntigen RNA sequence
WWOX-C15orf41chr1678198186chr15371005251019NMARASEAVGAAGAATGGATTTGGGCCAGGCTTAGT
WWOX-C15orf41chr1678198186chr15371005251221ARASEAVSRTGGATTTGGGCCAGGCTTAGTCATCTA
WWOX-C15orf41chr1678198186chr15371005251223ARASEAVSRILTGGATTTGGGCCAGGCTTAGTCATCTATTGGTA

check button mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs.
Fusion geneHchrHbpTchrTbpStart in +/-13AAEnd in +/-13AAFusionNeoAntigen peptideFusionNEoAntigen RNA sequence

Top

Information of the samples that have these potential fusion neoantigens of WWOX-C15orf41

check button These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens.
Cancer typeFusion geneHchrHbpHenstTchrTbpTenstSample
GBMWWOX-C15orf41chr1678198186ENST00000355860chr1537100525ENST00000437989TCGA-06-5415-01A

Top

Potential target of CAR-T therapy development for WWOX-C15orf41

check button Predicted 3D structure. We used RoseTTAFold.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features


* Minus value of BPloci means that the break point is located before the CDS.
- In-frame and retained 'Transmembrane'.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

check button Subcellular localization prediction of the transmembrane domain retained fusion proteins
* We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image.
HgeneHchrHbpHenstTgeneTchrTbpTenstDeepLoc result

Top

Related Drugs to WWOX-C15orf41

check button Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDrugSourcePMID

Top

Related Diseases to WWOX-C15orf41

check button Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDiseaseSourcePMID

check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource