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Center for Computational Systems Medicine level1
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Fusion Gene Summary

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Fusion Gene Sample Information

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Fusion ORF Analysis

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Fusion Amino Acid Sequences

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Fusion Protein Functional Features

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Fusion Protein-Protein Interaction

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Related drugs with this fusion protein

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Related disease with this fusion protein

Fusion Protein:ZDHHC20-KCNK1

Fusion Protein Summary

check button Fusion gene summary
Fusion partner gene informationFusion gene name: ZDHHC20-KCNK1
FusionPDB ID: 100793
FusionGDB2.0 ID: 100793
HgeneTgene
Gene symbol

ZDHHC20

KCNK1

Gene ID

253832

3775

Gene namezinc finger DHHC-type palmitoyltransferase 20potassium two pore domain channel subfamily K member 1
Synonyms4933421L13Rik|DHHC-20|DHHC20DPK|HOHO|K2P1|K2p1.1|KCNO1|TWIK-1|TWIK1
Cytomap

13q12.11

1q42.2

Type of geneprotein-codingprotein-coding
Descriptionpalmitoyltransferase ZDHHC20probable palmitoyltransferase ZDHHC20zinc finger DHHC domain-containing protein 20zinc finger DHHC-type containing 20potassium channel subfamily K member 1inward rectifying potassium channel protein TWIK-1potassium channel K2P1potassium channel KCNO1potassium channel, two pore domain subfamily K, member 1potassium inwardly-rectifying channel, subfamily K, member 1
Modification date2020031320200320
UniProtAcc.

Q96T54

Ensembl transtripts involved in fusion geneENST idsENST00000320220, ENST00000382466, 
ENST00000400590, ENST00000415724, 
ENST00000542645, ENST00000494731, 
ENST00000422251, 		ENST00000366620, 
ENST00000472190, ENST00000366621, 
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)* DoF score14 X 11 X 8=12326 X 3 X 5=90
# samples 166
** MAII scorelog2(16/1232*10)=-2.94485844580754
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(6/90*10)=-0.584962500721156
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)

PubMed: ZDHHC20 [Title/Abstract] AND KCNK1 [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)ZDHHC20(21965861)-KCNK1(233802341), # samples:1
Anticipated loss of major functional domain due to fusion event.ZDHHC20-KCNK1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
ZDHHC20-KCNK1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
ZDHHC20-KCNK1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
ZDHHC20-KCNK1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
ZDHHC20-KCNK1 seems lost the major protein functional domain in Tgene partner, which is a IUPHAR drug target due to the frame-shifted ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgeneZDHHC20

GO:0018230

peptidyl-L-cysteine S-palmitoylation

29326245

HgeneZDHHC20

GO:0018345

protein palmitoylation

23034182

TgeneKCNK1

GO:0035725

sodium ion transmembrane transport

21653227

TgeneKCNK1

GO:0071805

potassium ion transmembrane transport

21653227|22282804


check buttonFusion gene breakpoints across ZDHHC20 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check buttonFusion gene breakpoints across KCNK1 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure


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Fusion Gene Sample Information

check buttonFusion gene information from FusionGDB2.0.
check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4BLCATCGA-FD-A6TF-01AZDHHC20chr13

21965861

-KCNK1chr1

233802341

+


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Fusion ORF Analysis


check buttonFusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000382466ZDHHC20chr1321965861-ENST00000366621KCNK1chr1233802341+2478840141495493
ENST00000415724ZDHHC20chr1321965861-ENST00000366621KCNK1chr1233802341+246983151486493

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000382466ENST00000366621ZDHHC20chr1321965861-KCNK1chr1233802341+0.0002443410.9997557
ENST00000415724ENST00000366621ZDHHC20chr1321965861-KCNK1chr1233802341+0.0002395890.99976045

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Fusion Amino Acid Sequences


check button For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>100793_100793_1_ZDHHC20-KCNK1_ZDHHC20_chr13_21965861_ENST00000382466_KCNK1_chr1_233802341_ENST00000366621_length(amino acids)=493AA_BP=275
MDQQPPGASESPSPGLPRSPERWDAGLGSRQRNMAPWTLWRCCQRVVGWVPVLFITFVVVWSYYAYVVELCVFTIFGNEENGKTVVYLVA
FHLFFVMFVWSYWMTIFTSPASPSKEFYLSNSEKERYEKEFSQERQQEILRRAARALPIYTTSASKTIRYCEKCQLIKPDRAHHCSACDS
CILKMDHHCPWVNNCVGFSNYKFFLLFLLYSLLYCLFVAATVLEYFIKFWTNELTDTRAKFHVLFLFFVSAMFFISVLSLFSYHCWLVGK
NRTTIGYGHTVPLSDGGKAFCIIYSVIGIPFTLLFLTAVVQRITVHVTRRPVLYFHIRWGFSKQVVAIVHAVLLGFVTVSCFFFIPAAVF
SVLEDDWNFLESFYFCFISLSTIGLGDYVPGEGYNQKFRELYKIGITCYLLLGLIAMLVVLETFCELHELKKFRKMFYVKKDKDEDQVHI

--------------------------------------------------------------

>100793_100793_2_ZDHHC20-KCNK1_ZDHHC20_chr13_21965861_ENST00000415724_KCNK1_chr1_233802341_ENST00000366621_length(amino acids)=493AA_BP=275
MDQQPPGASESPSPGLPRSPERWDAGLGSRQRNMAPWTLWRCCQRVVGWVPVLFITFVVVWSYYAYVVELCVFTIFGNEENGKTVVYLVA
FHLFFVMFVWSYWMTIFTSPASPSKEFYLSNSEKERYEKEFSQERQQEILRRAARALPIYTTSASKTIRYCEKCQLIKPDRAHHCSACDS
CILKMDHHCPWVNNCVGFSNYKFFLLFLLYSLLYCLFVAATVLEYFIKFWTNELTDTRAKFHVLFLFFVSAMFFISVLSLFSYHCWLVGK
NRTTIGYGHTVPLSDGGKAFCIIYSVIGIPFTLLFLTAVVQRITVHVTRRPVLYFHIRWGFSKQVVAIVHAVLLGFVTVSCFFFIPAAVF
SVLEDDWNFLESFYFCFISLSTIGLGDYVPGEGYNQKFRELYKIGITCYLLLGLIAMLVVLETFCELHELKKFRKMFYVKKDKDEDQVHI

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Fusion Protein Functional Features


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr13:21965861/chr1:233802341)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
.KCNK1

Q96T54

FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.FUNCTION: Outward rectifying potassium channel. Produces rapidly activating and non-inactivating outward rectifier K(+) currents.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page

* Minus value of BPloci means that the break pointn is located before the CDS.
- Retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneZDHHC20chr13:21965861chr1:233802341ENST00000382466-812126_176242.33333333333334355.0DomainDHHC
HgeneZDHHC20chr13:21965861chr1:233802341ENST00000400590-813126_176242.33333333333334454.6666666666667DomainDHHC
HgeneZDHHC20chr13:21965861chr1:233802341ENST00000415724-812126_176242.33333333333334366.0DomainDHHC
HgeneZDHHC20chr13:21965861chr1:233802341ENST00000382466-812140_143242.33333333333334355.0RegionSubstrate binding
HgeneZDHHC20chr13:21965861chr1:233802341ENST00000400590-813140_143242.33333333333334454.6666666666667RegionSubstrate binding
HgeneZDHHC20chr13:21965861chr1:233802341ENST00000415724-812140_143242.33333333333334366.0RegionSubstrate binding
HgeneZDHHC20chr13:21965861chr1:233802341ENST00000382466-812191_207242.33333333333334355.0Topological domainLumenal
HgeneZDHHC20chr13:21965861chr1:233802341ENST00000382466-8121_14242.33333333333334355.0Topological domainCytoplasmic
HgeneZDHHC20chr13:21965861chr1:233802341ENST00000382466-81236_53242.33333333333334355.0Topological domainLumenal
HgeneZDHHC20chr13:21965861chr1:233802341ENST00000382466-81275_169242.33333333333334355.0Topological domainCytoplasmic
HgeneZDHHC20chr13:21965861chr1:233802341ENST00000400590-813191_207242.33333333333334454.6666666666667Topological domainLumenal
HgeneZDHHC20chr13:21965861chr1:233802341ENST00000400590-8131_14242.33333333333334454.6666666666667Topological domainCytoplasmic
HgeneZDHHC20chr13:21965861chr1:233802341ENST00000400590-81336_53242.33333333333334454.6666666666667Topological domainLumenal
HgeneZDHHC20chr13:21965861chr1:233802341ENST00000400590-81375_169242.33333333333334454.6666666666667Topological domainCytoplasmic
HgeneZDHHC20chr13:21965861chr1:233802341ENST00000415724-812191_207242.33333333333334366.0Topological domainLumenal
HgeneZDHHC20chr13:21965861chr1:233802341ENST00000415724-8121_14242.33333333333334366.0Topological domainCytoplasmic
HgeneZDHHC20chr13:21965861chr1:233802341ENST00000415724-81236_53242.33333333333334366.0Topological domainLumenal
HgeneZDHHC20chr13:21965861chr1:233802341ENST00000415724-81275_169242.33333333333334366.0Topological domainCytoplasmic
HgeneZDHHC20chr13:21965861chr1:233802341ENST00000382466-81215_35242.33333333333334355.0TransmembraneHelical
HgeneZDHHC20chr13:21965861chr1:233802341ENST00000382466-812170_190242.33333333333334355.0TransmembraneHelical
HgeneZDHHC20chr13:21965861chr1:233802341ENST00000382466-812208_231242.33333333333334355.0TransmembraneHelical
HgeneZDHHC20chr13:21965861chr1:233802341ENST00000382466-81254_74242.33333333333334355.0TransmembraneHelical
HgeneZDHHC20chr13:21965861chr1:233802341ENST00000400590-81315_35242.33333333333334454.6666666666667TransmembraneHelical
HgeneZDHHC20chr13:21965861chr1:233802341ENST00000400590-813170_190242.33333333333334454.6666666666667TransmembraneHelical
HgeneZDHHC20chr13:21965861chr1:233802341ENST00000400590-813208_231242.33333333333334454.6666666666667TransmembraneHelical
HgeneZDHHC20chr13:21965861chr1:233802341ENST00000400590-81354_74242.33333333333334454.6666666666667TransmembraneHelical
HgeneZDHHC20chr13:21965861chr1:233802341ENST00000415724-81215_35242.33333333333334366.0TransmembraneHelical
HgeneZDHHC20chr13:21965861chr1:233802341ENST00000415724-812170_190242.33333333333334366.0TransmembraneHelical
HgeneZDHHC20chr13:21965861chr1:233802341ENST00000415724-812208_231242.33333333333334366.0TransmembraneHelical
HgeneZDHHC20chr13:21965861chr1:233802341ENST00000415724-81254_74242.33333333333334366.0TransmembraneHelical
TgeneKCNK1chr13:21965861chr1:233802341ENST0000036662103212_224118.33333333333333337.0IntramembraneHelical%3B Name%3DPore helix 2
TgeneKCNK1chr13:21965861chr1:233802341ENST0000036662103225_231118.33333333333333337.0IntramembraneOntology_term=ECO:0000269
TgeneKCNK1chr13:21965861chr1:233802341ENST0000036662103117_122118.33333333333333337.0RegionSelectivity filter 1
TgeneKCNK1chr13:21965861chr1:233802341ENST0000036662103225_230118.33333333333333337.0RegionSelectivity filter 2
TgeneKCNK1chr13:21965861chr1:233802341ENST0000036662103293_299118.33333333333333337.0RegionImportant for intracellular retention in recycling endosomes
TgeneKCNK1chr13:21965861chr1:233802341ENST0000036662103123_132118.33333333333333337.0Topological domainExtracellular
TgeneKCNK1chr13:21965861chr1:233802341ENST0000036662103157_181118.33333333333333337.0Topological domainCytoplasmic
TgeneKCNK1chr13:21965861chr1:233802341ENST0000036662103203_211118.33333333333333337.0Topological domainExtracellular
TgeneKCNK1chr13:21965861chr1:233802341ENST0000036662103232_243118.33333333333333337.0Topological domainExtracellular
TgeneKCNK1chr13:21965861chr1:233802341ENST0000036662103268_336118.33333333333333337.0Topological domainCytoplasmic
TgeneKCNK1chr13:21965861chr1:233802341ENST0000036662103133_156118.33333333333333337.0TransmembraneHelical
TgeneKCNK1chr13:21965861chr1:233802341ENST0000036662103182_202118.33333333333333337.0TransmembraneHelical
TgeneKCNK1chr13:21965861chr1:233802341ENST0000036662103244_267118.33333333333333337.0TransmembraneHelical

- Not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneZDHHC20chr13:21965861chr1:233802341ENST00000422251-15126_1760131.0DomainDHHC
HgeneZDHHC20chr13:21965861chr1:233802341ENST00000422251-15140_1430131.0RegionSubstrate binding
HgeneZDHHC20chr13:21965861chr1:233802341ENST00000382466-812232_365242.33333333333334355.0Topological domainCytoplasmic
HgeneZDHHC20chr13:21965861chr1:233802341ENST00000400590-813232_365242.33333333333334454.6666666666667Topological domainCytoplasmic
HgeneZDHHC20chr13:21965861chr1:233802341ENST00000415724-812232_365242.33333333333334366.0Topological domainCytoplasmic
HgeneZDHHC20chr13:21965861chr1:233802341ENST00000422251-15191_2070131.0Topological domainLumenal
HgeneZDHHC20chr13:21965861chr1:233802341ENST00000422251-151_140131.0Topological domainCytoplasmic
HgeneZDHHC20chr13:21965861chr1:233802341ENST00000422251-15232_3650131.0Topological domainCytoplasmic
HgeneZDHHC20chr13:21965861chr1:233802341ENST00000422251-1536_530131.0Topological domainLumenal
HgeneZDHHC20chr13:21965861chr1:233802341ENST00000422251-1575_1690131.0Topological domainCytoplasmic
HgeneZDHHC20chr13:21965861chr1:233802341ENST00000422251-1515_350131.0TransmembraneHelical
HgeneZDHHC20chr13:21965861chr1:233802341ENST00000422251-15170_1900131.0TransmembraneHelical
HgeneZDHHC20chr13:21965861chr1:233802341ENST00000422251-15208_2310131.0TransmembraneHelical
HgeneZDHHC20chr13:21965861chr1:233802341ENST00000422251-1554_740131.0TransmembraneHelical
TgeneKCNK1chr13:21965861chr1:233802341ENST0000036662103104_116118.33333333333333337.0IntramembraneHelical%3B Name%3DPore helix 1
TgeneKCNK1chr13:21965861chr1:233802341ENST00000366621031_20118.33333333333333337.0Topological domainCytoplasmic
TgeneKCNK1chr13:21965861chr1:233802341ENST000003666210342_103118.33333333333333337.0Topological domainExtracellular
TgeneKCNK1chr13:21965861chr1:233802341ENST000003666210321_41118.33333333333333337.0TransmembraneHelical


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Fusion Protein-Protein Interaction


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)
GenePPI interactors


check button Protein-protein interactors based on sequence similarity (STRING)
GeneSTRING network
ZDHHC20
KCNK1


check button - Retained interactions in fusion protein (protein functional feature from UniProt).
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost interactions due to fusion (protein functional feature from UniProt).
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs to ZDHHC20-KCNK1


check button Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDrugSourcePMID

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Related Diseases to ZDHHC20-KCNK1


check button Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDiseaseSourcePMID

check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource