UTHEALTH HOME    ABOUT SBMI    A-Z    WEBMAIL    INSIDE THE UNIVERSITY
FusionGDB Logo

Home

Download

Statistics

Examples

Help

Contact

Terms of Use
Center for Computational Systems Medicine level1
leaf

Fusion Gene Summary

leaf

Fusion Gene Sample Information

leaf

Fusion ORF Analysis

leaf

Fusion Amino Acid Sequences

leaf

Fusion Protein Functional Features

leaf

Fusion Protein-Protein Interaction

leaf

Related drugs with this fusion protein

leaf

Related disease with this fusion protein

Fusion Protein:BRWD1-GNAI3

Fusion Protein Summary

check button Fusion gene summary
Fusion partner gene informationFusion gene name: BRWD1-GNAI3
FusionPDB ID: 10346
FusionGDB2.0 ID: 10346
HgeneTgene
Gene symbol

BRWD1

GNAI3

Gene ID

54014

2773

Gene namebromodomain and WD repeat domain containing 1G protein subunit alpha i3
SynonymsC21orf107|DCAF19|N143|WDR9|WRD987U6|ARCND1
Cytomap

21q22.2

1p13.3

Type of geneprotein-codingprotein-coding
Descriptionbromodomain and WD repeat-containing protein 1WD repeat protein WDR9-form2WD repeat-containing protein 9transcriptional unit N143guanine nucleotide-binding protein G(i) subunit alphag(i) alpha-3guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3guanine nucleotide-binding protein G(k) subunit alpha
Modification date2020031320200322
UniProtAcc

Q9NSI6

P08754

Ensembl transtripts involved in fusion geneENST idsENST00000333229, ENST00000342449, 
ENST00000380800, ENST00000341322, 
ENST00000470108, 		ENST00000369851, 
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)* DoF score28 X 28 X 10=784033 X 8 X 14=3696
# samples 3235
** MAII scorelog2(32/7840*10)=-4.61470984411521
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(35/3696*10)=-3.40053792958373
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)

PubMed: BRWD1 [Title/Abstract] AND GNAI3 [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)BRWD1(40670357)-GNAI3(110116358), # samples:1
Anticipated loss of major functional domain due to fusion event.BRWD1-GNAI3 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
BRWD1-GNAI3 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
BRWD1-GNAI3 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
BRWD1-GNAI3 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
TgeneGNAI3

GO:0007193

adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway

19478087

TgeneGNAI3

GO:0007212

dopamine receptor signaling pathway

19478087

TgeneGNAI3

GO:0046039

GTP metabolic process

19478087

TgeneGNAI3

GO:0051301

cell division

17635935


check buttonFusion gene breakpoints across BRWD1 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check buttonFusion gene breakpoints across GNAI3 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure


Top

Fusion Gene Sample Information

check buttonFusion gene information from FusionGDB2.0.
check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4BRCATCGA-D8-A1XTBRWD1chr21

40670357

-GNAI3chr1

110116358

+


Top

Fusion ORF Analysis


check buttonFusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000333229BRWD1chr2140670357-ENST00000369851GNAI3chr1110116358+36556772351623462
ENST00000342449BRWD1chr2140670357-ENST00000369851GNAI3chr1110116358+3406428791374431
ENST00000380800BRWD1chr2140670357-ENST00000369851GNAI3chr1110116358+342644861394462

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000333229ENST00000369851BRWD1chr2140670357-GNAI3chr1110116358+0.0002034610.99979657
ENST00000342449ENST00000369851BRWD1chr2140670357-GNAI3chr1110116358+0.0001863990.9998136
ENST00000380800ENST00000369851BRWD1chr2140670357-GNAI3chr1110116358+0.0001849750.99981505

Top

Fusion Amino Acid Sequences


check button For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>10346_10346_1_BRWD1-GNAI3_BRWD1_chr21_40670357_ENST00000333229_GNAI3_chr1_110116358_ENST00000369851_length(amino acids)=462AA_BP=147
MRRGDASPVRPRRGLHAPLARSLPPPAPRPAMAEPSSARRPVPLIESELYFLIARYLSAGPCRRAAQVLVQELEQYQLLPKRLDWEGNEH
NRSYEELVLSNKHVAPDHLLQICQRIGPMLDKEIPPSISRVTSLLGAGRQSLLRTAKGAGESGKSTIVKQMKIIHEDGYSEDECKQYKVV
VYSNTIQSIIAIIRAMGRLKIDFGEAARADDARQLFVLAGSAEEGVMTPELAGVIKRLWRDGGVQACFSRSREYQLNDSASYYLNDLDRI
SQSNYIPTQQDVLRTRVKTTGIVETHFTFKDLYFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDS
ICNNKWFTETSIILFLNKKDLFEEKIKRSPLTICYPEYTGSNTYEEAAAYIQCQFEDLNRRKDTKEIYTHFTCATDTKNVQFVFDAVTDV

--------------------------------------------------------------

>10346_10346_2_BRWD1-GNAI3_BRWD1_chr21_40670357_ENST00000342449_GNAI3_chr1_110116358_ENST00000369851_length(amino acids)=431AA_BP=116
MAEPSSARRPVPLIESELYFLIARYLSAGPCRRAAQVLVQELEQYQLLPKRLDWEGNEHNRSYEELVLSNKHVAPDHLLQICQRIGPMLD
KEIPPSISRVTSLLGAGRQSLLRTAKGAGESGKSTIVKQMKIIHEDGYSEDECKQYKVVVYSNTIQSIIAIIRAMGRLKIDFGEAARADD
ARQLFVLAGSAEEGVMTPELAGVIKRLWRDGGVQACFSRSREYQLNDSASYYLNDLDRISQSNYIPTQQDVLRTRVKTTGIVETHFTFKD
LYFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTETSIILFLNKKDLFEEKIKRSPL

--------------------------------------------------------------

>10346_10346_3_BRWD1-GNAI3_BRWD1_chr21_40670357_ENST00000380800_GNAI3_chr1_110116358_ENST00000369851_length(amino acids)=462AA_BP=147
MRRGDASPVRPRRGLHAPLARSLPPPAPRPAMAEPSSARRPVPLIESELYFLIARYLSAGPCRRAAQVLVQELEQYQLLPKRLDWEGNEH
NRSYEELVLSNKHVAPDHLLQICQRIGPMLDKEIPPSISRVTSLLGAGRQSLLRTAKGAGESGKSTIVKQMKIIHEDGYSEDECKQYKVV
VYSNTIQSIIAIIRAMGRLKIDFGEAARADDARQLFVLAGSAEEGVMTPELAGVIKRLWRDGGVQACFSRSREYQLNDSASYYLNDLDRI
SQSNYIPTQQDVLRTRVKTTGIVETHFTFKDLYFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDS
ICNNKWFTETSIILFLNKKDLFEEKIKRSPLTICYPEYTGSNTYEEAAAYIQCQFEDLNRRKDTKEIYTHFTCATDTKNVQFVFDAVTDV

--------------------------------------------------------------

Top

Fusion Protein Functional Features


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr21:40670357/chr1:110116358)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
BRWD1

Q9NSI6

GNAI3

P08754

FUNCTION: May be a transcriptional activator. May be involved in chromatin remodeling (By similarity). Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape. {ECO:0000250, ECO:0000269|PubMed:21834987}.FUNCTION: Heterotrimeric guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (PubMed:8774883, PubMed:18434541, PubMed:19478087). Signaling is mediated via effector proteins, such as adenylate cyclase. Inhibits adenylate cyclase activity, leading to decreased intracellular cAMP levels (PubMed:19478087). Stimulates the activity of receptor-regulated K(+) channels (PubMed:2535845). The active GTP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division (PubMed:17635935). {ECO:0000269|PubMed:17635935, ECO:0000269|PubMed:18434541, ECO:0000269|PubMed:2535845, ECO:0000269|PubMed:8774883}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page

* Minus value of BPloci means that the break pointn is located before the CDS.
- Retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
TgeneGNAI3chr21:40670357chr1:110116358ENST0000036985109150_15139.333333333333336948.6666666666666Nucleotide bindingGTP
TgeneGNAI3chr21:40670357chr1:110116358ENST0000036985109175_18139.333333333333336948.6666666666666Nucleotide bindingGTP
TgeneGNAI3chr21:40670357chr1:110116358ENST0000036985109200_20439.333333333333336948.6666666666666Nucleotide bindingGTP
TgeneGNAI3chr21:40670357chr1:110116358ENST0000036985109269_27239.333333333333336948.6666666666666Nucleotide bindingGTP
TgeneGNAI3chr21:40670357chr1:110116358ENST000003698510943_4839.333333333333336948.6666666666666Nucleotide bindingGTP
TgeneGNAI3chr21:40670357chr1:110116358ENST0000036985109173_18139.333333333333336948.6666666666666RegionG2 motif
TgeneGNAI3chr21:40670357chr1:110116358ENST0000036985109196_20539.333333333333336948.6666666666666RegionG3 motif
TgeneGNAI3chr21:40670357chr1:110116358ENST0000036985109265_27239.333333333333336948.6666666666666RegionG4 motif
TgeneGNAI3chr21:40670357chr1:110116358ENST0000036985109324_32939.333333333333336948.6666666666666RegionG5 motif

- Not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneBRWD1chr21:40670357chr1:110116358ENST00000333229-5421539_1548116.333333333333332321.0Compositional biasNote=Poly-Ser
HgeneBRWD1chr21:40670357chr1:110116358ENST00000333229-5421687_1692116.333333333333332321.0Compositional biasNote=Poly-Glu
HgeneBRWD1chr21:40670357chr1:110116358ENST00000333229-542661_668116.333333333333332321.0Compositional biasNote=Poly-Gln
HgeneBRWD1chr21:40670357chr1:110116358ENST00000333229-542821_824116.333333333333332321.0Compositional biasNote=Poly-Ser
HgeneBRWD1chr21:40670357chr1:110116358ENST00000341322-151539_15480121.0Compositional biasNote=Poly-Ser
HgeneBRWD1chr21:40670357chr1:110116358ENST00000341322-151687_16920121.0Compositional biasNote=Poly-Glu
HgeneBRWD1chr21:40670357chr1:110116358ENST00000341322-15661_6680121.0Compositional biasNote=Poly-Gln
HgeneBRWD1chr21:40670357chr1:110116358ENST00000341322-15821_8240121.0Compositional biasNote=Poly-Ser
HgeneBRWD1chr21:40670357chr1:110116358ENST00000342449-5411539_1548116.333333333333332270.0Compositional biasNote=Poly-Ser
HgeneBRWD1chr21:40670357chr1:110116358ENST00000342449-5411687_1692116.333333333333332270.0Compositional biasNote=Poly-Glu
HgeneBRWD1chr21:40670357chr1:110116358ENST00000342449-541661_668116.333333333333332270.0Compositional biasNote=Poly-Gln
HgeneBRWD1chr21:40670357chr1:110116358ENST00000342449-541821_824116.333333333333332270.0Compositional biasNote=Poly-Ser
HgeneBRWD1chr21:40670357chr1:110116358ENST00000380800-5421539_1548116.333333333333332200.0Compositional biasNote=Poly-Ser
HgeneBRWD1chr21:40670357chr1:110116358ENST00000380800-5421687_1692116.333333333333332200.0Compositional biasNote=Poly-Glu
HgeneBRWD1chr21:40670357chr1:110116358ENST00000380800-542661_668116.333333333333332200.0Compositional biasNote=Poly-Gln
HgeneBRWD1chr21:40670357chr1:110116358ENST00000380800-542821_824116.333333333333332200.0Compositional biasNote=Poly-Ser
HgeneBRWD1chr21:40670357chr1:110116358ENST00000333229-5421177_1247116.333333333333332321.0DomainBromo 1
HgeneBRWD1chr21:40670357chr1:110116358ENST00000333229-5421330_1400116.333333333333332321.0DomainBromo 2
HgeneBRWD1chr21:40670357chr1:110116358ENST00000341322-151177_12470121.0DomainBromo 1
HgeneBRWD1chr21:40670357chr1:110116358ENST00000341322-151330_14000121.0DomainBromo 2
HgeneBRWD1chr21:40670357chr1:110116358ENST00000342449-5411177_1247116.333333333333332270.0DomainBromo 1
HgeneBRWD1chr21:40670357chr1:110116358ENST00000342449-5411330_1400116.333333333333332270.0DomainBromo 2
HgeneBRWD1chr21:40670357chr1:110116358ENST00000380800-5421177_1247116.333333333333332200.0DomainBromo 1
HgeneBRWD1chr21:40670357chr1:110116358ENST00000380800-5421330_1400116.333333333333332200.0DomainBromo 2
HgeneBRWD1chr21:40670357chr1:110116358ENST00000333229-542184_223116.333333333333332321.0RepeatNote=WD 1
HgeneBRWD1chr21:40670357chr1:110116358ENST00000333229-542226_265116.333333333333332321.0RepeatNote=WD 2
HgeneBRWD1chr21:40670357chr1:110116358ENST00000333229-542268_311116.333333333333332321.0RepeatNote=WD 3
HgeneBRWD1chr21:40670357chr1:110116358ENST00000333229-542322_365116.333333333333332321.0RepeatNote=WD 4
HgeneBRWD1chr21:40670357chr1:110116358ENST00000333229-542366_405116.333333333333332321.0RepeatNote=WD 5
HgeneBRWD1chr21:40670357chr1:110116358ENST00000333229-542424_463116.333333333333332321.0RepeatNote=WD 6
HgeneBRWD1chr21:40670357chr1:110116358ENST00000333229-542466_506116.333333333333332321.0RepeatNote=WD 7
HgeneBRWD1chr21:40670357chr1:110116358ENST00000333229-542514_553116.333333333333332321.0RepeatNote=WD 8
HgeneBRWD1chr21:40670357chr1:110116358ENST00000341322-15184_2230121.0RepeatNote=WD 1
HgeneBRWD1chr21:40670357chr1:110116358ENST00000341322-15226_2650121.0RepeatNote=WD 2
HgeneBRWD1chr21:40670357chr1:110116358ENST00000341322-15268_3110121.0RepeatNote=WD 3
HgeneBRWD1chr21:40670357chr1:110116358ENST00000341322-15322_3650121.0RepeatNote=WD 4
HgeneBRWD1chr21:40670357chr1:110116358ENST00000341322-15366_4050121.0RepeatNote=WD 5
HgeneBRWD1chr21:40670357chr1:110116358ENST00000341322-15424_4630121.0RepeatNote=WD 6
HgeneBRWD1chr21:40670357chr1:110116358ENST00000341322-15466_5060121.0RepeatNote=WD 7
HgeneBRWD1chr21:40670357chr1:110116358ENST00000341322-15514_5530121.0RepeatNote=WD 8
HgeneBRWD1chr21:40670357chr1:110116358ENST00000342449-541184_223116.333333333333332270.0RepeatNote=WD 1
HgeneBRWD1chr21:40670357chr1:110116358ENST00000342449-541226_265116.333333333333332270.0RepeatNote=WD 2
HgeneBRWD1chr21:40670357chr1:110116358ENST00000342449-541268_311116.333333333333332270.0RepeatNote=WD 3
HgeneBRWD1chr21:40670357chr1:110116358ENST00000342449-541322_365116.333333333333332270.0RepeatNote=WD 4
HgeneBRWD1chr21:40670357chr1:110116358ENST00000342449-541366_405116.333333333333332270.0RepeatNote=WD 5
HgeneBRWD1chr21:40670357chr1:110116358ENST00000342449-541424_463116.333333333333332270.0RepeatNote=WD 6
HgeneBRWD1chr21:40670357chr1:110116358ENST00000342449-541466_506116.333333333333332270.0RepeatNote=WD 7
HgeneBRWD1chr21:40670357chr1:110116358ENST00000342449-541514_553116.333333333333332270.0RepeatNote=WD 8
HgeneBRWD1chr21:40670357chr1:110116358ENST00000380800-542184_223116.333333333333332200.0RepeatNote=WD 1
HgeneBRWD1chr21:40670357chr1:110116358ENST00000380800-542226_265116.333333333333332200.0RepeatNote=WD 2
HgeneBRWD1chr21:40670357chr1:110116358ENST00000380800-542268_311116.333333333333332200.0RepeatNote=WD 3
HgeneBRWD1chr21:40670357chr1:110116358ENST00000380800-542322_365116.333333333333332200.0RepeatNote=WD 4
HgeneBRWD1chr21:40670357chr1:110116358ENST00000380800-542366_405116.333333333333332200.0RepeatNote=WD 5
HgeneBRWD1chr21:40670357chr1:110116358ENST00000380800-542424_463116.333333333333332200.0RepeatNote=WD 6
HgeneBRWD1chr21:40670357chr1:110116358ENST00000380800-542466_506116.333333333333332200.0RepeatNote=WD 7
HgeneBRWD1chr21:40670357chr1:110116358ENST00000380800-542514_553116.333333333333332200.0RepeatNote=WD 8
TgeneGNAI3chr21:40670357chr1:110116358ENST000003698510932_35439.333333333333336948.6666666666666DomainG-alpha
TgeneGNAI3chr21:40670357chr1:110116358ENST000003698510935_4839.333333333333336948.6666666666666RegionG1 motif


Top

Fusion Protein-Protein Interaction


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)
GenePPI interactors


check button Protein-protein interactors based on sequence similarity (STRING)
GeneSTRING network
BRWD1
GNAI3


check button - Retained interactions in fusion protein (protein functional feature from UniProt).
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost interactions due to fusion (protein functional feature from UniProt).
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


Top

Related Drugs to BRWD1-GNAI3


check button Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDrugSourcePMID

Top

Related Diseases to BRWD1-GNAI3


check button Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDiseaseSourcePMID

check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource