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Fusion Protein:CARM1-PLA2G4C |
Fusion Protein Summary |
 Fusion gene summary |
| Fusion partner gene information | Fusion gene name: CARM1-PLA2G4C | FusionPDB ID: 13061 | FusionGDB2.0 ID: 13061 | Hgene | Tgene | Gene symbol | CARM1 | PLA2G4C | Gene ID | 10498 | 8605 |
| Gene name | coactivator associated arginine methyltransferase 1 | phospholipase A2 group IVC | |
| Synonyms | PRMT4 | CPLA2-gamma | |
| Cytomap | 19p13.2 | 19q13.33 | |
| Type of gene | protein-coding | protein-coding | |
| Description | histone-arginine methyltransferase CARM1protein arginine N-methyltransferase 4 | cytosolic phospholipase A2 gammaphospholipase A2, group IVC (cytosolic, calcium-independent) | |
| Modification date | 20200313 | 20200313 | |
| UniProtAcc | Q86X55 | . | |
| Ensembl transtripts involved in fusion gene | ENST ids | ENST00000327064, ENST00000344150, | ENST00000354276, ENST00000413144, ENST00000599921, ENST00000596510, ENST00000599111, |
| Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0) | * DoF score | 14 X 10 X 10=1400 | 5 X 7 X 3=105 |
| # samples | 20 | 6 | |
| ** MAII score | log2(20/1400*10)=-2.8073549220576 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | log2(6/105*10)=-0.807354922057604 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | |
| Context (manual curation of fusion genes in FusionPDB) | PubMed: CARM1 [Title/Abstract] AND PLA2G4C [Title/Abstract] AND fusion [Title/Abstract] | ||
| Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0) | CARM1(10982598)-PLA2G4C(48558306), # samples:5 | ||
| Anticipated loss of major functional domain due to fusion event. | CARM1-PLA2G4C seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. CARM1-PLA2G4C seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. CARM1-PLA2G4C seems lost the major protein functional domain in Hgene partner, which is a epigenetic factor due to the frame-shifted ORF. CARM1-PLA2G4C seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF. CARM1-PLA2G4C seems lost the major protein functional domain in Hgene partner, which is a IUPHAR drug target due to the frame-shifted ORF. CARM1-PLA2G4C seems lost the major protein functional domain in Tgene partner, which is a cell metabolism gene due to the frame-shifted ORF. CARM1-PLA2G4C seems lost the major protein functional domain in Tgene partner, which is a IUPHAR drug target due to the frame-shifted ORF. | ||
| * DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
| Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
| Partner | Gene | GO ID | GO term | PubMed ID |
| Hgene | CARM1 | GO:0016571 | histone methylation | 19405910 |
| Tgene | PLA2G4C | GO:0046475 | glycerophospholipid catabolic process | 10085124 |
| Fusion gene breakpoints across CARM1 (5'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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| Fusion gene breakpoints across PLA2G4C (3'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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Fusion Gene Sample Information |
| Fusion gene information from FusionGDB2.0. |
| Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0) * All genome coordinats were lifted-over on hg19. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| Source | Disease | Sample | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| ChimerDB4 | ESCA | TCGA-L5-A891-01A | CARM1 | chr19 | 10982598 | - | PLA2G4C | chr19 | 48558306 | - |
| ChimerDB4 | ESCA | TCGA-L5-A891-01A | CARM1 | chr19 | 10982598 | + | PLA2G4C | chr19 | 48558306 | - |
| ChimerDB4 | ESCA | TCGA-L5-A891 | CARM1 | chr19 | 10982598 | + | PLA2G4C | chr19 | 48551645 | - |
| ChimerDB4 | ESCA | TCGA-L5-A891 | CARM1 | chr19 | 10982598 | + | PLA2G4C | chr19 | 48556404 | - |
| ChimerDB4 | ESCA | TCGA-L5-A891 | CARM1 | chr19 | 10982598 | + | PLA2G4C | chr19 | 48558306 | - |
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Fusion ORF Analysis |
| Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
| ENST00000327064 | CARM1 | chr19 | 10982598 | + | ENST00000599111 | PLA2G4C | chr19 | 48556404 | - | 1107 | 410 | 46 | 606 | 186 |
| ENST00000327064 | CARM1 | chr19 | 10982598 | + | ENST00000413144 | PLA2G4C | chr19 | 48556404 | - | 607 | 410 | 46 | 606 | 187 |
| ENST00000327064 | CARM1 | chr19 | 10982598 | + | ENST00000599921 | PLA2G4C | chr19 | 48556404 | - | 607 | 410 | 46 | 606 | 187 |
| ENST00000344150 | CARM1 | chr19 | 10982598 | + | ENST00000599111 | PLA2G4C | chr19 | 48556404 | - | 917 | 220 | 0 | 416 | 138 |
| ENST00000344150 | CARM1 | chr19 | 10982598 | + | ENST00000413144 | PLA2G4C | chr19 | 48556404 | - | 417 | 220 | 0 | 416 | 138 |
| ENST00000344150 | CARM1 | chr19 | 10982598 | + | ENST00000599921 | PLA2G4C | chr19 | 48556404 | - | 417 | 220 | 0 | 416 | 138 |
| DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
| ENST00000327064 | ENST00000599111 | CARM1 | chr19 | 10982598 | + | PLA2G4C | chr19 | 48556404 | - | 0.005248617 | 0.9947514 |
| ENST00000327064 | ENST00000413144 | CARM1 | chr19 | 10982598 | + | PLA2G4C | chr19 | 48556404 | - | 0.00359034 | 0.9964097 |
| ENST00000327064 | ENST00000599921 | CARM1 | chr19 | 10982598 | + | PLA2G4C | chr19 | 48556404 | - | 0.00359034 | 0.9964097 |
| ENST00000344150 | ENST00000599111 | CARM1 | chr19 | 10982598 | + | PLA2G4C | chr19 | 48556404 | - | 0.00412043 | 0.99587953 |
| ENST00000344150 | ENST00000413144 | CARM1 | chr19 | 10982598 | + | PLA2G4C | chr19 | 48556404 | - | 0.006223999 | 0.99377596 |
| ENST00000344150 | ENST00000599921 | CARM1 | chr19 | 10982598 | + | PLA2G4C | chr19 | 48556404 | - | 0.006223999 | 0.99377596 |
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Fusion Amino Acid Sequences |
| For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones. |
| >FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP >13061_13061_1_CARM1-PLA2G4C_CARM1_chr19_10982598_ENST00000327064_PLA2G4C_chr19_48556404_ENST00000413144_length(amino acids)=187AA_BP=11 MHGGCGGGSGSGGGGGGGGGGGGGGGGSGGGLGPGAAAAAAGPGAGSKMAAAAAAVGPGAGGAGSAVPGGAGPCATVSVFPGARLLTIGD ANGEIQRHAEQQALRLEVRAGPDSAGIALYSRDIEAWSDTYDTFKLADTYTLDVVVLLLALAKKNVRENKKKILRELMNVAGLYYPKDSA -------------------------------------------------------------- >13061_13061_2_CARM1-PLA2G4C_CARM1_chr19_10982598_ENST00000327064_PLA2G4C_chr19_48556404_ENST00000599111_length(amino acids)=186AA_BP=11 MHGGCGGGSGSGGGGGGGGGGGGGGGGSGGGLGPGAAAAAAGPGAGSKMAAAAAAVGPGAGGAGSAVPGGAGPCATVSVFPGARLLTIGD ANGEIQRHAEQQALRLEVRAGPDSAGIALYSRDIEAWSDTYDTFKLADTYTLDVVVLLLALAKKNVRENKKKILRELMNVAGLYYPKDSA -------------------------------------------------------------- >13061_13061_3_CARM1-PLA2G4C_CARM1_chr19_10982598_ENST00000327064_PLA2G4C_chr19_48556404_ENST00000599921_length(amino acids)=187AA_BP=11 MHGGCGGGSGSGGGGGGGGGGGGGGGGSGGGLGPGAAAAAAGPGAGSKMAAAAAAVGPGAGGAGSAVPGGAGPCATVSVFPGARLLTIGD ANGEIQRHAEQQALRLEVRAGPDSAGIALYSRDIEAWSDTYDTFKLADTYTLDVVVLLLALAKKNVRENKKKILRELMNVAGLYYPKDSA -------------------------------------------------------------- >13061_13061_4_CARM1-PLA2G4C_CARM1_chr19_10982598_ENST00000344150_PLA2G4C_chr19_48556404_ENST00000413144_length(amino acids)=138AA_BP=72 MAAAAAAVGPGAGGAGSAVPGGAGPCATVSVFPGARLLTIGDANGEIQRHAEQQALRLEVRAGPDSAGIALYSRDIEAWSDTYDTFKLAD -------------------------------------------------------------- >13061_13061_5_CARM1-PLA2G4C_CARM1_chr19_10982598_ENST00000344150_PLA2G4C_chr19_48556404_ENST00000599111_length(amino acids)=138AA_BP=72 MAAAAAAVGPGAGGAGSAVPGGAGPCATVSVFPGARLLTIGDANGEIQRHAEQQALRLEVRAGPDSAGIALYSRDIEAWSDTYDTFKLAD -------------------------------------------------------------- >13061_13061_6_CARM1-PLA2G4C_CARM1_chr19_10982598_ENST00000344150_PLA2G4C_chr19_48556404_ENST00000599921_length(amino acids)=138AA_BP=72 MAAAAAAVGPGAGGAGSAVPGGAGPCATVSVFPGARLLTIGDANGEIQRHAEQQALRLEVRAGPDSAGIALYSRDIEAWSDTYDTFKLAD -------------------------------------------------------------- |
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Fusion Protein Functional Features |
| Four levels of functional features of fusion genes Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr19:10982598/chr19:48558306) - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. - How to search 1. Put your fusion gene symbol. 2. Press the tab key until there will be shown the breakpoint information filled. 4. Go down and press 'Search' tab twice. 4. Go down to have the hyperlink of the search result. 5. Click the hyperlink. 6. See the FGviewer result for your fusion gene. |
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| Main function of each fusion partner protein. (from UniProt) |
| Hgene | Tgene |
| CARM1 | . |
| FUNCTION: Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activate transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Acts as coactivator for PPARG, promotes adipocyte differentiation and the accumulation of brown fat tissue. Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at 'Arg-2142', which may loosen its interaction with NCOA2/GRIP1, and at 'Arg-580' and 'Arg-604' in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs. {ECO:0000269|PubMed:16497732, ECO:0000269|PubMed:19405910}. | FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes. |
| Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at * Minus value of BPloci means that the break pointn is located before the CDS. |
| - Retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| - Not-retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| Hgene | CARM1 | chr19:10982598 | chr19:48556404 | ENST00000327064 | + | 1 | 16 | 146_453 | 73.33333333333333 | 609.0 | Domain | SAM-dependent MTase PRMT-type |
| Hgene | CARM1 | chr19:10982598 | chr19:48556404 | ENST00000344150 | + | 1 | 15 | 146_453 | 73.33333333333333 | 586.0 | Domain | SAM-dependent MTase PRMT-type |
| Hgene | CARM1 | chr19:10982598 | chr19:48556404 | ENST00000327064 | + | 1 | 16 | 499_608 | 73.33333333333333 | 609.0 | Region | Transactivation domain |
| Hgene | CARM1 | chr19:10982598 | chr19:48556404 | ENST00000344150 | + | 1 | 15 | 499_608 | 73.33333333333333 | 586.0 | Region | Transactivation domain |
| Tgene | PLA2G4C | chr19:10982598 | chr19:48556404 | ENST00000354276 | 14 | 17 | 1_541 | 476.3333333333333 | 662.3333333333334 | Domain | PLA2c | |
| Tgene | PLA2G4C | chr19:10982598 | chr19:48556404 | ENST00000413144 | 13 | 16 | 1_541 | 476.3333333333333 | 542.0 | Domain | PLA2c | |
| Tgene | PLA2G4C | chr19:10982598 | chr19:48556404 | ENST00000599111 | 14 | 17 | 1_541 | 486.3333333333333 | 552.0 | Domain | PLA2c | |
| Tgene | PLA2G4C | chr19:10982598 | chr19:48556404 | ENST00000599921 | 14 | 17 | 1_541 | 476.3333333333333 | 542.0 | Domain | PLA2c | |
| Tgene | PLA2G4C | chr19:10982598 | chr19:48556404 | ENST00000354276 | 14 | 17 | 260_292 | 476.3333333333333 | 662.3333333333334 | Region | Required for lipid droplet localization | |
| Tgene | PLA2G4C | chr19:10982598 | chr19:48556404 | ENST00000413144 | 13 | 16 | 260_292 | 476.3333333333333 | 542.0 | Region | Required for lipid droplet localization | |
| Tgene | PLA2G4C | chr19:10982598 | chr19:48556404 | ENST00000599111 | 14 | 17 | 260_292 | 486.3333333333333 | 552.0 | Region | Required for lipid droplet localization | |
| Tgene | PLA2G4C | chr19:10982598 | chr19:48556404 | ENST00000599921 | 14 | 17 | 260_292 | 476.3333333333333 | 542.0 | Region | Required for lipid droplet localization |
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Fusion Protein-Protein Interaction |
| Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in |
| Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160) |
| Gene | PPI interactors |
| Protein-protein interactors based on sequence similarity (STRING) |
| Gene | STRING network |
| CARM1 | |
| PLA2G4C |
| - Retained interactions in fusion protein (protein functional feature from UniProt). |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Still interaction with |
| - Lost interactions due to fusion (protein functional feature from UniProt). |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
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Related Drugs to CARM1-PLA2G4C |
| Drugs used for this fusion-positive patient. (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
| Hgene | Tgene | Drug | Source | PMID |
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Related Diseases to CARM1-PLA2G4C |
| Diseases that have this fusion gene. (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
| Hgene | Tgene | Disease | Source | PMID |
| Diseases associated with fusion partners. (DisGeNet 4.0) |
| Partner | Gene | Disease ID | Disease name | # pubmeds | Source |
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