| UTHEALTH HOME ABOUT SBMI A-Z WEBMAIL INSIDE THE UNIVERSITY |
|
|||||||
|
Fusion Protein:CHEK2-THOC5 |
Fusion Protein Summary |
 Fusion gene summary |
| Fusion partner gene information | Fusion gene name: CHEK2-THOC5 | FusionPDB ID: 16420 | FusionGDB2.0 ID: 16420 | Hgene | Tgene | Gene symbol | CHEK2 | THOC5 | Gene ID | 11200 | 8563 |
| Gene name | checkpoint kinase 2 | THO complex 5 | |
| Synonyms | CDS1|CHK2|HuCds1|LFS2|PP1425|RAD53|hCds1 | C22orf19|Fmip|PK1.3|fSAP79 | |
| Cytomap | 22q12.1 | 22q12.2 | |
| Type of gene | protein-coding | protein-coding | |
| Description | serine/threonine-protein kinase Chk2CHK2 checkpoint homologcds1 homologcheckpoint-like protein CHK2 | THO complex subunit 5 homologFms-interacting proteinNF2/meningioma region protein pK1.3PP39.2functional spliceosome-associated protein 79hTREX90placental protein 39.2 | |
| Modification date | 20200320 | 20200313 | |
| UniProtAcc | O96017 | . | |
| Ensembl transtripts involved in fusion gene | ENST ids | ENST00000382566, ENST00000328354, ENST00000348295, ENST00000382565, ENST00000382578, ENST00000382580, ENST00000402731, ENST00000403642, ENST00000404276, ENST00000405598, ENST00000544772, ENST00000464581, | ENST00000397871, ENST00000397872, ENST00000397873, ENST00000490103, |
| Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0) | * DoF score | 3 X 3 X 3=27 | 6 X 6 X 4=144 |
| # samples | 3 | 6 | |
| ** MAII score | log2(3/27*10)=0.15200309344505 effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs). DoF>8 and MAII>0 | log2(6/144*10)=-1.26303440583379 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | |
| Context (manual curation of fusion genes in FusionPDB) | PubMed: CHEK2 [Title/Abstract] AND THOC5 [Title/Abstract] AND fusion [Title/Abstract] | ||
| Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0) | CHEK2(29085123)-THOC5(29932727), # samples:2 | ||
| Anticipated loss of major functional domain due to fusion event. | CHEK2-THOC5 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. CHEK2-THOC5 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. CHEK2-THOC5 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. CHEK2-THOC5 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. CHEK2-THOC5 seems lost the major protein functional domain in Hgene partner, which is a CGC due to the frame-shifted ORF. CHEK2-THOC5 seems lost the major protein functional domain in Hgene partner, which is a IUPHAR drug target due to the frame-shifted ORF. CHEK2-THOC5 seems lost the major protein functional domain in Hgene partner, which is a kinase due to the frame-shifted ORF. CHEK2-THOC5 seems lost the major protein functional domain in Hgene partner, which is a tumor suppressor due to the frame-shifted ORF. CHEK2-THOC5 seems lost the major protein functional domain in Tgene partner, which is a essential gene due to the frame-shifted ORF. | ||
| * DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
| Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
| Partner | Gene | GO ID | GO term | PubMed ID |
| Hgene | CHEK2 | GO:0006355 | regulation of transcription, DNA-templated | 12717439 |
| Hgene | CHEK2 | GO:0006468 | protein phosphorylation | 12717439 |
| Hgene | CHEK2 | GO:0006974 | cellular response to DNA damage stimulus | 24550317 |
| Hgene | CHEK2 | GO:0008630 | intrinsic apoptotic signaling pathway in response to DNA damage | 12402044 |
| Hgene | CHEK2 | GO:0042770 | signal transduction in response to DNA damage | 14744935 |
| Hgene | CHEK2 | GO:0045893 | positive regulation of transcription, DNA-templated | 17101782 |
| Hgene | CHEK2 | GO:0046777 | protein autophosphorylation | 16794575|18644861 |
| Hgene | CHEK2 | GO:0050821 | protein stabilization | 12717439 |
| Tgene | THOC5 | GO:0006406 | mRNA export from nucleus | 17190602 |
| Tgene | THOC5 | GO:0030224 | monocyte differentiation | 19015024 |
| Tgene | THOC5 | GO:0046784 | viral mRNA export from host cell nucleus | 18974867 |
| Fusion gene breakpoints across CHEK2 (5'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
 |
| Fusion gene breakpoints across THOC5 (3'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
 |
Top |
Fusion Gene Sample Information |
| Fusion gene information from FusionGDB2.0. |
| Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0) * All genome coordinats were lifted-over on hg19. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| Source | Disease | Sample | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| ChimerDB4 | UCS | TCGA-NF-A4WX-01A | CHEK2 | chr22 | 29085123 | - | THOC5 | chr22 | 29932727 | - |
| ChimerDB4 | UCS | TCGA-NF-A4WX | CHEK2 | chr22 | 29085123 | - | THOC5 | chr22 | 29932727 | - |
Top |
Fusion ORF Analysis |
| Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
| ENST00000544772 | CHEK2 | chr22 | 29085123 | - | ENST00000490103 | THOC5 | chr22 | 29932727 | - | 6347 | 2316 | 2317 | 3768 | 483 |
| ENST00000544772 | CHEK2 | chr22 | 29085123 | - | ENST00000397871 | THOC5 | chr22 | 29932727 | - | 4058 | 2316 | 2317 | 3768 | 483 |
| ENST00000544772 | CHEK2 | chr22 | 29085123 | - | ENST00000397872 | THOC5 | chr22 | 29932727 | - | 4058 | 2316 | 2317 | 3768 | 483 |
| ENST00000544772 | CHEK2 | chr22 | 29085123 | - | ENST00000397873 | THOC5 | chr22 | 29932727 | - | 3868 | 2316 | 2317 | 3768 | 483 |
| DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
| ENST00000544772 | ENST00000490103 | CHEK2 | chr22 | 29085123 | - | THOC5 | chr22 | 29932727 | - | 0.000988825 | 0.99901116 |
| ENST00000544772 | ENST00000397871 | CHEK2 | chr22 | 29085123 | - | THOC5 | chr22 | 29932727 | - | 0.002415247 | 0.9975847 |
| ENST00000544772 | ENST00000397872 | CHEK2 | chr22 | 29085123 | - | THOC5 | chr22 | 29932727 | - | 0.002415247 | 0.9975847 |
| ENST00000544772 | ENST00000397873 | CHEK2 | chr22 | 29085123 | - | THOC5 | chr22 | 29932727 | - | 0.002550976 | 0.997449 |
Top |
Fusion Amino Acid Sequences |
| For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones. |
| >FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP >16420_16420_1_CHEK2-THOC5_CHEK2_chr22_29085123_ENST00000544772_THOC5_chr22_29932727_ENST00000397871_length(amino acids)=483AA_BP=1 MAEKYRECLSNKEKILKEIEVKKEYLSSLQPRLNSIMQASLPVQEYLFMPFDQAHKQYETARHLPPPLYVLFVQATAYGQACDKTLSVAI EGSVDEAKALFKPPEDSQDDESDSDAEEEQTTKRRRPTLGVQLDDKRKEMLKRHPLSVMLDLKCKDDSVLHLTFYYLMNLNIMTVKAKVT TAMELITPISAGDLLSPDSVLSCLYPGDHGKKTPNPANQYQFDKVGILTLSDYVLELGHPYLWVQKLGGLHFPKEQPQQTVIADHSLSAS HMETTMKLLKTRVQSRLALHKQFASLEHGIVPVTSDCQYLFPAKVVSRLVKWVTVAHEDYMELHFTKDIVDAGLAGDTNLYYMALIERGT AKLQAAVVLNPGYSSIPPVFQLCLNWKGEKTNSNDDNIRAMEGEVNVCYKELCGPWPSHQLLTNQLQRLCVLLDVYLETESHDDSVEGPK -------------------------------------------------------------- >16420_16420_2_CHEK2-THOC5_CHEK2_chr22_29085123_ENST00000544772_THOC5_chr22_29932727_ENST00000397872_length(amino acids)=483AA_BP=1 MAEKYRECLSNKEKILKEIEVKKEYLSSLQPRLNSIMQASLPVQEYLFMPFDQAHKQYETARHLPPPLYVLFVQATAYGQACDKTLSVAI EGSVDEAKALFKPPEDSQDDESDSDAEEEQTTKRRRPTLGVQLDDKRKEMLKRHPLSVMLDLKCKDDSVLHLTFYYLMNLNIMTVKAKVT TAMELITPISAGDLLSPDSVLSCLYPGDHGKKTPNPANQYQFDKVGILTLSDYVLELGHPYLWVQKLGGLHFPKEQPQQTVIADHSLSAS HMETTMKLLKTRVQSRLALHKQFASLEHGIVPVTSDCQYLFPAKVVSRLVKWVTVAHEDYMELHFTKDIVDAGLAGDTNLYYMALIERGT AKLQAAVVLNPGYSSIPPVFQLCLNWKGEKTNSNDDNIRAMEGEVNVCYKELCGPWPSHQLLTNQLQRLCVLLDVYLETESHDDSVEGPK -------------------------------------------------------------- >16420_16420_3_CHEK2-THOC5_CHEK2_chr22_29085123_ENST00000544772_THOC5_chr22_29932727_ENST00000397873_length(amino acids)=483AA_BP=1 MAEKYRECLSNKEKILKEIEVKKEYLSSLQPRLNSIMQASLPVQEYLFMPFDQAHKQYETARHLPPPLYVLFVQATAYGQACDKTLSVAI EGSVDEAKALFKPPEDSQDDESDSDAEEEQTTKRRRPTLGVQLDDKRKEMLKRHPLSVMLDLKCKDDSVLHLTFYYLMNLNIMTVKAKVT TAMELITPISAGDLLSPDSVLSCLYPGDHGKKTPNPANQYQFDKVGILTLSDYVLELGHPYLWVQKLGGLHFPKEQPQQTVIADHSLSAS HMETTMKLLKTRVQSRLALHKQFASLEHGIVPVTSDCQYLFPAKVVSRLVKWVTVAHEDYMELHFTKDIVDAGLAGDTNLYYMALIERGT AKLQAAVVLNPGYSSIPPVFQLCLNWKGEKTNSNDDNIRAMEGEVNVCYKELCGPWPSHQLLTNQLQRLCVLLDVYLETESHDDSVEGPK -------------------------------------------------------------- >16420_16420_4_CHEK2-THOC5_CHEK2_chr22_29085123_ENST00000544772_THOC5_chr22_29932727_ENST00000490103_length(amino acids)=483AA_BP=1 MAEKYRECLSNKEKILKEIEVKKEYLSSLQPRLNSIMQASLPVQEYLFMPFDQAHKQYETARHLPPPLYVLFVQATAYGQACDKTLSVAI EGSVDEAKALFKPPEDSQDDESDSDAEEEQTTKRRRPTLGVQLDDKRKEMLKRHPLSVMLDLKCKDDSVLHLTFYYLMNLNIMTVKAKVT TAMELITPISAGDLLSPDSVLSCLYPGDHGKKTPNPANQYQFDKVGILTLSDYVLELGHPYLWVQKLGGLHFPKEQPQQTVIADHSLSAS HMETTMKLLKTRVQSRLALHKQFASLEHGIVPVTSDCQYLFPAKVVSRLVKWVTVAHEDYMELHFTKDIVDAGLAGDTNLYYMALIERGT AKLQAAVVLNPGYSSIPPVFQLCLNWKGEKTNSNDDNIRAMEGEVNVCYKELCGPWPSHQLLTNQLQRLCVLLDVYLETESHDDSVEGPK -------------------------------------------------------------- |
Top |
Fusion Protein Functional Features |
| Four levels of functional features of fusion genes Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr22:29085123/chr22:29932727) - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. - How to search 1. Put your fusion gene symbol. 2. Press the tab key until there will be shown the breakpoint information filled. 4. Go down and press 'Search' tab twice. 4. Go down to have the hyperlink of the search result. 5. Click the hyperlink. 6. See the FGviewer result for your fusion gene. |
 |
| Main function of each fusion partner protein. (from UniProt) |
| Hgene | Tgene |
| CHEK2 | . |
| FUNCTION: Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. May also negatively regulate cell cycle progression during unperturbed cell cycles. Following activation, phosphorylates numerous effectors preferentially at the consensus sequence [L-X-R-X-X-S/T]. Regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C, inhibiting their activity. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. May also phosphorylate NEK6 which is involved in G2/M cell cycle arrest. Regulates DNA repair through phosphorylation of BRCA2, enhancing the association of RAD51 with chromatin which promotes DNA repair by homologous recombination. Also stimulates the transcription of genes involved in DNA repair (including BRCA2) through the phosphorylation and activation of the transcription factor FOXM1. Regulates apoptosis through the phosphorylation of p53/TP53, MDM4 and PML. Phosphorylation of p53/TP53 at 'Ser-20' by CHEK2 may alleviate inhibition by MDM2, leading to accumulation of active p53/TP53. Phosphorylation of MDM4 may also reduce degradation of p53/TP53. Also controls the transcription of pro-apoptotic genes through phosphorylation of the transcription factor E2F1. Tumor suppressor, it may also have a DNA damage-independent function in mitotic spindle assembly by phosphorylating BRCA1. Its absence may be a cause of the chromosomal instability observed in some cancer cells. Promotes the CCAR2-SIRT1 association and is required for CCAR2-mediated SIRT1 inhibition (PubMed:25361978). {ECO:0000250|UniProtKB:Q9Z265, ECO:0000269|PubMed:10097108, ECO:0000269|PubMed:10724175, ECO:0000269|PubMed:11298456, ECO:0000269|PubMed:12402044, ECO:0000269|PubMed:12607004, ECO:0000269|PubMed:12717439, ECO:0000269|PubMed:12810724, ECO:0000269|PubMed:16163388, ECO:0000269|PubMed:17101782, ECO:0000269|PubMed:17380128, ECO:0000269|PubMed:17715138, ECO:0000269|PubMed:18317453, ECO:0000269|PubMed:18644861, ECO:0000269|PubMed:18728393, ECO:0000269|PubMed:20364141, ECO:0000269|PubMed:25361978, ECO:0000269|PubMed:25619829, ECO:0000269|PubMed:9836640, ECO:0000269|PubMed:9889122}. | FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes. |
| Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at * Minus value of BPloci means that the break pointn is located before the CDS. |
| - Retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000328354 | - | 14 | 15 | 113_175 | 514.0 | 544.0 | Domain | FHA |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000328354 | - | 14 | 15 | 220_486 | 514.0 | 544.0 | Domain | Protein kinase |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000348295 | - | 13 | 14 | 113_175 | 485.0 | 515.0 | Domain | FHA |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000348295 | - | 13 | 14 | 220_486 | 485.0 | 515.0 | Domain | Protein kinase |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000382566 | - | 13 | 14 | 113_175 | 493.3333333333333 | 276.6666666666667 | Domain | FHA |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000382566 | - | 13 | 14 | 220_486 | 493.3333333333333 | 276.6666666666667 | Domain | Protein kinase |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000382578 | - | 12 | 13 | 113_175 | 423.0 | 453.0 | Domain | FHA |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000382580 | - | 15 | 16 | 113_175 | 557.0 | 587.0 | Domain | FHA |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000382580 | - | 15 | 16 | 220_486 | 557.0 | 587.0 | Domain | Protein kinase |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000402731 | - | 12 | 13 | 113_175 | 485.0 | 515.0 | Domain | FHA |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000402731 | - | 12 | 13 | 220_486 | 485.0 | 515.0 | Domain | Protein kinase |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000403642 | - | 11 | 12 | 113_175 | 423.0 | 453.0 | Domain | FHA |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000404276 | - | 13 | 14 | 113_175 | 514.0 | 544.0 | Domain | FHA |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000404276 | - | 13 | 14 | 220_486 | 514.0 | 544.0 | Domain | Protein kinase |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000405598 | - | 15 | 16 | 113_175 | 514.0 | 544.0 | Domain | FHA |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000405598 | - | 15 | 16 | 220_486 | 514.0 | 544.0 | Domain | Protein kinase |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000544772 | - | 15 | 16 | 113_175 | 293.0 | 323.0 | Domain | FHA |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000328354 | - | 14 | 15 | 227_234 | 514.0 | 544.0 | Nucleotide binding | Note=ATP |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000328354 | - | 14 | 15 | 302_308 | 514.0 | 544.0 | Nucleotide binding | Note=ATP |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000328354 | - | 14 | 15 | 351_352 | 514.0 | 544.0 | Nucleotide binding | Note=ATP |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000348295 | - | 13 | 14 | 227_234 | 485.0 | 515.0 | Nucleotide binding | Note=ATP |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000348295 | - | 13 | 14 | 302_308 | 485.0 | 515.0 | Nucleotide binding | Note=ATP |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000348295 | - | 13 | 14 | 351_352 | 485.0 | 515.0 | Nucleotide binding | Note=ATP |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000382566 | - | 13 | 14 | 227_234 | 493.3333333333333 | 276.6666666666667 | Nucleotide binding | Note=ATP |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000382566 | - | 13 | 14 | 302_308 | 493.3333333333333 | 276.6666666666667 | Nucleotide binding | Note=ATP |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000382566 | - | 13 | 14 | 351_352 | 493.3333333333333 | 276.6666666666667 | Nucleotide binding | Note=ATP |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000382578 | - | 12 | 13 | 227_234 | 423.0 | 453.0 | Nucleotide binding | Note=ATP |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000382578 | - | 12 | 13 | 302_308 | 423.0 | 453.0 | Nucleotide binding | Note=ATP |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000382578 | - | 12 | 13 | 351_352 | 423.0 | 453.0 | Nucleotide binding | Note=ATP |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000382580 | - | 15 | 16 | 227_234 | 557.0 | 587.0 | Nucleotide binding | Note=ATP |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000382580 | - | 15 | 16 | 302_308 | 557.0 | 587.0 | Nucleotide binding | Note=ATP |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000382580 | - | 15 | 16 | 351_352 | 557.0 | 587.0 | Nucleotide binding | Note=ATP |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000402731 | - | 12 | 13 | 227_234 | 485.0 | 515.0 | Nucleotide binding | Note=ATP |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000402731 | - | 12 | 13 | 302_308 | 485.0 | 515.0 | Nucleotide binding | Note=ATP |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000402731 | - | 12 | 13 | 351_352 | 485.0 | 515.0 | Nucleotide binding | Note=ATP |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000403642 | - | 11 | 12 | 227_234 | 423.0 | 453.0 | Nucleotide binding | Note=ATP |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000403642 | - | 11 | 12 | 302_308 | 423.0 | 453.0 | Nucleotide binding | Note=ATP |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000403642 | - | 11 | 12 | 351_352 | 423.0 | 453.0 | Nucleotide binding | Note=ATP |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000404276 | - | 13 | 14 | 227_234 | 514.0 | 544.0 | Nucleotide binding | Note=ATP |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000404276 | - | 13 | 14 | 302_308 | 514.0 | 544.0 | Nucleotide binding | Note=ATP |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000404276 | - | 13 | 14 | 351_352 | 514.0 | 544.0 | Nucleotide binding | Note=ATP |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000405598 | - | 15 | 16 | 227_234 | 514.0 | 544.0 | Nucleotide binding | Note=ATP |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000405598 | - | 15 | 16 | 302_308 | 514.0 | 544.0 | Nucleotide binding | Note=ATP |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000405598 | - | 15 | 16 | 351_352 | 514.0 | 544.0 | Nucleotide binding | Note=ATP |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000544772 | - | 15 | 16 | 227_234 | 293.0 | 323.0 | Nucleotide binding | Note=ATP |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000328354 | - | 14 | 15 | 368_394 | 514.0 | 544.0 | Region | Note=T-loop/activation segment |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000348295 | - | 13 | 14 | 368_394 | 485.0 | 515.0 | Region | Note=T-loop/activation segment |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000382566 | - | 13 | 14 | 368_394 | 493.3333333333333 | 276.6666666666667 | Region | Note=T-loop/activation segment |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000382578 | - | 12 | 13 | 368_394 | 423.0 | 453.0 | Region | Note=T-loop/activation segment |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000382580 | - | 15 | 16 | 368_394 | 557.0 | 587.0 | Region | Note=T-loop/activation segment |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000402731 | - | 12 | 13 | 368_394 | 485.0 | 515.0 | Region | Note=T-loop/activation segment |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000403642 | - | 11 | 12 | 368_394 | 423.0 | 453.0 | Region | Note=T-loop/activation segment |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000404276 | - | 13 | 14 | 368_394 | 514.0 | 544.0 | Region | Note=T-loop/activation segment |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000405598 | - | 15 | 16 | 368_394 | 514.0 | 544.0 | Region | Note=T-loop/activation segment |
| - Not-retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000382578 | - | 12 | 13 | 220_486 | 423.0 | 453.0 | Domain | Protein kinase |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000403642 | - | 11 | 12 | 220_486 | 423.0 | 453.0 | Domain | Protein kinase |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000544772 | - | 15 | 16 | 220_486 | 293.0 | 323.0 | Domain | Protein kinase |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000544772 | - | 15 | 16 | 302_308 | 293.0 | 323.0 | Nucleotide binding | Note=ATP |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000544772 | - | 15 | 16 | 351_352 | 293.0 | 323.0 | Nucleotide binding | Note=ATP |
| Hgene | CHEK2 | chr22:29085123 | chr22:29932727 | ENST00000544772 | - | 15 | 16 | 368_394 | 293.0 | 323.0 | Region | Note=T-loop/activation segment |
| Tgene | THOC5 | chr22:29085123 | chr22:29932727 | ENST00000397871 | 5 | 20 | 7_10 | 199.66666666666666 | 684.0 | Motif | Nuclear localization signal | |
| Tgene | THOC5 | chr22:29085123 | chr22:29932727 | ENST00000397872 | 6 | 21 | 7_10 | 199.66666666666666 | 684.0 | Motif | Nuclear localization signal | |
| Tgene | THOC5 | chr22:29085123 | chr22:29932727 | ENST00000397873 | 6 | 21 | 7_10 | 199.66666666666666 | 684.0 | Motif | Nuclear localization signal | |
| Tgene | THOC5 | chr22:29085123 | chr22:29932727 | ENST00000490103 | 5 | 20 | 7_10 | 199.66666666666666 | 684.0 | Motif | Nuclear localization signal |
Top |
Fusion Protein Structures |
| PDB and CIF files of the predicted fusion proteins * Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format. |
| Fusion protein PDB link (fusion AA seq ID in FusionPDB) | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | AA seq | Len(AA seq) |
| PDB file >>>1012_CHEK2_29085123_THOC5_29932727_1012_CHEK2_29085123_THOC5_29932727_ranked_0.pdb | CHEK2 | 29085123 | 29085123 | ENST00000397873 | THOC5 | chr22 | 29932727 | - | MAEKYRECLSNKEKILKEIEVKKEYLSSLQPRLNSIMQASLPVQEYLFMPFDQAHKQYETARHLPPPLYVLFVQATAYGQACDKTLSVAI EGSVDEAKALFKPPEDSQDDESDSDAEEEQTTKRRRPTLGVQLDDKRKEMLKRHPLSVMLDLKCKDDSVLHLTFYYLMNLNIMTVKAKVT TAMELITPISAGDLLSPDSVLSCLYPGDHGKKTPNPANQYQFDKVGILTLSDYVLELGHPYLWVQKLGGLHFPKEQPQQTVIADHSLSAS HMETTMKLLKTRVQSRLALHKQFASLEHGIVPVTSDCQYLFPAKVVSRLVKWVTVAHEDYMELHFTKDIVDAGLAGDTNLYYMALIERGT AKLQAAVVLNPGYSSIPPVFQLCLNWKGEKTNSNDDNIRAMEGEVNVCYKELCGPWPSHQLLTNQLQRLCVLLDVYLETESHDDSVEGPK | 483 |
Top |
pLDDT score distribution |
| pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2 * AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. |
CHEK2_pLDDT.png  |
THOC5_pLDDT.png  |
| pLDDT score distribution of the predicted fusion protein structures from AlphaFold2 * AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. |
 |
Top |
Ramachandran Plot of Fusion Protein Structure |
| Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide. |
| Fusion AA seq ID in FusionPDB and their Ramachandran plots |
Top |
Fusion Protein-Protein Interaction |
| Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in |
| Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160) |
| Gene | PPI interactors |
| Protein-protein interactors based on sequence similarity (STRING) |
| Gene | STRING network |
| CHEK2 | |
| THOC5 |
| - Retained interactions in fusion protein (protein functional feature from UniProt). |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Still interaction with |
| - Lost interactions due to fusion (protein functional feature from UniProt). |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
| Tgene | THOC5 | chr22:29085123 | chr22:29932727 | ENST00000397871 | 5 | 20 | 2_144 | 199.66666666666666 | 684.0 | CSF1R | |
| Tgene | THOC5 | chr22:29085123 | chr22:29932727 | ENST00000397872 | 6 | 21 | 2_144 | 199.66666666666666 | 684.0 | CSF1R | |
| Tgene | THOC5 | chr22:29085123 | chr22:29932727 | ENST00000397873 | 6 | 21 | 2_144 | 199.66666666666666 | 684.0 | CSF1R | |
| Tgene | THOC5 | chr22:29085123 | chr22:29932727 | ENST00000490103 | 5 | 20 | 2_144 | 199.66666666666666 | 684.0 | CSF1R | |
| Tgene | THOC5 | chr22:29085123 | chr22:29932727 | ENST00000397871 | 5 | 20 | 2_199 | 199.66666666666666 | 684.0 | THOC7 | |
| Tgene | THOC5 | chr22:29085123 | chr22:29932727 | ENST00000397872 | 6 | 21 | 2_199 | 199.66666666666666 | 684.0 | THOC7 | |
| Tgene | THOC5 | chr22:29085123 | chr22:29932727 | ENST00000397873 | 6 | 21 | 2_199 | 199.66666666666666 | 684.0 | THOC7 | |
| Tgene | THOC5 | chr22:29085123 | chr22:29932727 | ENST00000490103 | 5 | 20 | 2_199 | 199.66666666666666 | 684.0 | THOC7 |
Top |
Related Drugs to CHEK2-THOC5 |
| Drugs used for this fusion-positive patient. (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
| Hgene | Tgene | Drug | Source | PMID |
Top |
Related Diseases to CHEK2-THOC5 |
| Diseases that have this fusion gene. (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
| Hgene | Tgene | Disease | Source | PMID |
| Diseases associated with fusion partners. (DisGeNet 4.0) |
| Partner | Gene | Disease ID | Disease name | # pubmeds | Source |
Copyright 2021-Present -The University of Texas Health Science Center at Houston (UTHealth)
Web File Viewing | Emergency Information |Campus Carry|Site Policies