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	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein Structure
	pLDDT scores
	Ramachandran Plot of Fusion Protein Structure
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:ACTN4-APLP1

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: ACTN4-APLP1
	FusionPDB ID: 1763
	FusionGDB2.0 ID: 1763
		Hgene	Tgene
	Gene symbol	ACTN4	APLP1
	Gene ID	81	333
	Gene name	actinin alpha 4	amyloid beta precursor like protein 1
	Synonyms	ACTININ-4\|FSGS\|FSGS1	APLP
	Cytomap	19q13.2	19q13.12
	Type of gene	protein-coding	protein-coding
	Description	alpha-actinin-4focal segmental glomerulosclerosis 1non-muscle alpha-actinin 4	amyloid-like protein 1amyloid beta (A4) precursor-like protein 1amyloid precursor-like protein 1
	Modification date	20200327	20200313
	UniProtAcc	O43707	P51693
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000252699, ENST00000390009, ENST00000424234, ENST00000497637,	ENST00000589298, ENST00000586861, ENST00000221891, ENST00000537454,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	42 X 60 X 18=45360	2 X 3 X 2=12
	# samples	77	3
	** MAII score	log2(77/45360*10)=-5.88041838424733 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(3/12*10)=1.32192809488736 effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs). DoF>8 and MAII>0
Context (manual curation of fusion genes in FusionPDB)	PubMed: ACTN4 [Title/Abstract] AND APLP1 [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	ACTN4(39138547)-APLP1(36360569), # samples:2
Anticipated loss of major functional domain due to fusion event.	ACTN4-APLP1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. ACTN4-APLP1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. ACTN4-APLP1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. ACTN4-APLP1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	ACTN4	GO:0033209	tumor necrosis factor-mediated signaling pathway	25411248
Hgene	ACTN4	GO:0035357	peroxisome proliferator activated receptor signaling pathway	22351778
Hgene	ACTN4	GO:0048384	retinoic acid receptor signaling pathway	22351778
Hgene	ACTN4	GO:0051272	positive regulation of cellular component movement	9508771
Tgene	APLP1	GO:0071874	cellular response to norepinephrine stimulus	16531006
Tgene	APLP1	GO:0106072	negative regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway	16531006

Fusion gene breakpoints across ACTN4 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across APLP1 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	LUSC	TCGA-34-5241-01A	ACTN4	chr19	39138547	+	APLP1	chr19	36360569	+
ChimerDB4	LUSC	TCGA-34-5241	ACTN4	chr19	39138547	+	APLP1	chr19	36360569	+
ChimerDB4	LUSC	TCGA-34-5241	ACTN4	chr19	39138547	+	APLP1	chr19	36361798	+

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000252699	ACTN4	chr19	39138547	+	ENST00000537454	APLP1	chr19	36360569	+	2129	238	76	2043	655
ENST00000252699	ACTN4	chr19	39138547	+	ENST00000221891	APLP1	chr19	36360569	+	2394	238	76	2046	656
ENST00000424234	ACTN4	chr19	39138547	+	ENST00000537454	APLP1	chr19	36360569	+	2112	221	59	2026	655
ENST00000424234	ACTN4	chr19	39138547	+	ENST00000221891	APLP1	chr19	36360569	+	2377	221	59	2029	656
ENST00000390009	ACTN4	chr19	39138547	+	ENST00000537454	APLP1	chr19	36360569	+	2061	170	8	1975	655
ENST00000390009	ACTN4	chr19	39138547	+	ENST00000221891	APLP1	chr19	36360569	+	2326	170	8	1978	656
ENST00000252699	ACTN4	chr19	39138547	+	ENST00000537454	APLP1	chr19	36361798	+	1985	238	76	1899	607
ENST00000252699	ACTN4	chr19	39138547	+	ENST00000221891	APLP1	chr19	36361798	+	2250	238	76	1902	608
ENST00000252699	ACTN4	chr19	39138547	+	ENST00000586861	APLP1	chr19	36361798	+	1952	238	76	1899	607
ENST00000424234	ACTN4	chr19	39138547	+	ENST00000537454	APLP1	chr19	36361798	+	1968	221	59	1882	607
ENST00000424234	ACTN4	chr19	39138547	+	ENST00000221891	APLP1	chr19	36361798	+	2233	221	59	1885	608
ENST00000424234	ACTN4	chr19	39138547	+	ENST00000586861	APLP1	chr19	36361798	+	1935	221	59	1882	607
ENST00000390009	ACTN4	chr19	39138547	+	ENST00000537454	APLP1	chr19	36361798	+	1917	170	8	1831	607
ENST00000390009	ACTN4	chr19	39138547	+	ENST00000221891	APLP1	chr19	36361798	+	2182	170	8	1834	608
ENST00000390009	ACTN4	chr19	39138547	+	ENST00000586861	APLP1	chr19	36361798	+	1884	170	8	1831	607

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000252699	ENST00000537454	ACTN4	chr19	39138547	+	APLP1	chr19	36360569	+	0.03819703	0.96180296
ENST00000252699	ENST00000221891	ACTN4	chr19	39138547	+	APLP1	chr19	36360569	+	0.020756818	0.97924316
ENST00000424234	ENST00000537454	ACTN4	chr19	39138547	+	APLP1	chr19	36360569	+	0.035767473	0.9642325
ENST00000424234	ENST00000221891	ACTN4	chr19	39138547	+	APLP1	chr19	36360569	+	0.019474668	0.9805253
ENST00000390009	ENST00000537454	ACTN4	chr19	39138547	+	APLP1	chr19	36360569	+	0.03637172	0.9636283
ENST00000390009	ENST00000221891	ACTN4	chr19	39138547	+	APLP1	chr19	36360569	+	0.01952934	0.98047066
ENST00000252699	ENST00000537454	ACTN4	chr19	39138547	+	APLP1	chr19	36361798	+	0.018230371	0.9817696
ENST00000252699	ENST00000221891	ACTN4	chr19	39138547	+	APLP1	chr19	36361798	+	0.010444982	0.989555
ENST00000252699	ENST00000586861	ACTN4	chr19	39138547	+	APLP1	chr19	36361798	+	0.017082484	0.9829175
ENST00000424234	ENST00000537454	ACTN4	chr19	39138547	+	APLP1	chr19	36361798	+	0.017379628	0.9826203
ENST00000424234	ENST00000221891	ACTN4	chr19	39138547	+	APLP1	chr19	36361798	+	0.009536834	0.9904632
ENST00000424234	ENST00000586861	ACTN4	chr19	39138547	+	APLP1	chr19	36361798	+	0.016298518	0.9837015
ENST00000390009	ENST00000537454	ACTN4	chr19	39138547	+	APLP1	chr19	36361798	+	0.017144892	0.9828551
ENST00000390009	ENST00000221891	ACTN4	chr19	39138547	+	APLP1	chr19	36361798	+	0.009918394	0.9900816
ENST00000390009	ENST00000586861	ACTN4	chr19	39138547	+	APLP1	chr19	36361798	+	0.016055666	0.98394436

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>1763_1763_1_ACTN4-APLP1_ACTN4_chr19_39138547_ENST00000252699_APLP1_chr19_36360569_ENST00000221891_length(amino acids)=656AA_BP=54
MVDYHAANQSYQYGPSSAGNGAGGGGSMGDYMAQEDDWDRDLLLDPAWEKQQRKAPGSAQVAGLCGRLTLHRDLRTGRWEPDPQRSRRCL
RDPQRVLEYCRQMYPELQIARVEQATQAIPMERWCGGSRSGSCAHPHHQVVPFRCLPGEFVSEALLVPEGCRFLHQERMDQCESSTRRHQ
EAQEACSSQGLILHGSGMLLPCGSDRFRGVEYVCCPPPGTPDPSGTAVGDPSTRSWPPGSRVEGAEDEEEEESFPQPVDDYFVEPPQAEE
EEETVPPPSSHTLAVVGKVTPTPRPTDGVDIYFGMPGEISEHEGFLRAKMDLEERRMRQINEVMREWAMADNQSKNLPKADRQALNEHFQ
SILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLAALQADPPQAERVLLALRRYLRAEQKEQRHTLRHYQHVAAVDPEKAQQM
RFQVHTHLQVIEERVNQSLGLLDQNPHLAQELRPQIQELLHSEHLGPSELEAPAPGGSSEDKGGLQPPDSKDADTPMTLPKGSTEQDAAS
PEKEKMNPLEQYERKVNASVPRGFPFHSSEIQRDELAPAGTGVSREAVSGLLIMGAGGGSLIVLSMLLLRRKKPYGAISHGVVEVDPMLT

--------------------------------------------------------------

>1763_1763_2_ACTN4-APLP1_ACTN4_chr19_39138547_ENST00000252699_APLP1_chr19_36360569_ENST00000537454_length(amino acids)=655AA_BP=54
MVDYHAANQSYQYGPSSAGNGAGGGGSMGDYMAQEDDWDRDLLLDPAWEKQQRKAPGSAQVAGLCGRLTLHRDLRTGRWEPDPQRSRRCL
RDPQRVLEYCRQMYPELQIARVEQATQAIPMERWCGGSRSGSCAHPHHQVVPFRCLPGEFVSEALLVPEGCRFLHQERMDQCESSTRRHQ
EAQEACSSQGLILHGSGMLLPCGSDRFRGVEYVCCPPPGTPDPSGTAVGDPSTRSWPPGSRVEGAEDEEEEESFPQPVDDYFVEPPQAEE
EEETVPPPSSHTLAVVGKVTPTPRPTDGVDIYFGMPGEISEHEGFLRAKMDLEERRMRQINEVMREWAMADNQSKNLPKADRQALNEHFQ
SILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLAALQADPPQAERVLLALRRYLRAEQKEQRHTLRHYQHVAAVDPEKAQQM
RFQVHTHLQVIEERVNQSLGLLDQNPHLAQELRPQIQELLHSEHLGPSELEAPAPGGSSEDKGGLQPPDSKDDTPMTLPKGSTEQDAASP
EKEKMNPLEQYERKVNASVPRGFPFHSSEIQRDELAPAGTGVSREAVSGLLIMGAGGGSLIVLSMLLLRRKKPYGAISHGVVEVDPMLTL

--------------------------------------------------------------

>1763_1763_3_ACTN4-APLP1_ACTN4_chr19_39138547_ENST00000252699_APLP1_chr19_36361798_ENST00000221891_length(amino acids)=608AA_BP=54
MVDYHAANQSYQYGPSSAGNGAGGGGSMGDYMAQEDDWDRDLLLDPAWEKQQRKMYPELQIARVEQATQAIPMERWCGGSRSGSCAHPHH
QVVPFRCLPGEFVSEALLVPEGCRFLHQERMDQCESSTRRHQEAQEACSSQGLILHGSGMLLPCGSDRFRGVEYVCCPPPGTPDPSGTAV
GDPSTRSWPPGSRVEGAEDEEEEESFPQPVDDYFVEPPQAEEEEETVPPPSSHTLAVVGKVTPTPRPTDGVDIYFGMPGEISEHEGFLRA
KMDLEERRMRQINEVMREWAMADNQSKNLPKADRQALNEHFQSILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLAALQADP
PQAERVLLALRRYLRAEQKEQRHTLRHYQHVAAVDPEKAQQMRFQVHTHLQVIEERVNQSLGLLDQNPHLAQELRPQIQELLHSEHLGPS
ELEAPAPGGSSEDKGGLQPPDSKDADTPMTLPKGSTEQDAASPEKEKMNPLEQYERKVNASVPRGFPFHSSEIQRDELAPAGTGVSREAV

--------------------------------------------------------------

>1763_1763_4_ACTN4-APLP1_ACTN4_chr19_39138547_ENST00000252699_APLP1_chr19_36361798_ENST00000537454_length(amino acids)=607AA_BP=54
MVDYHAANQSYQYGPSSAGNGAGGGGSMGDYMAQEDDWDRDLLLDPAWEKQQRKMYPELQIARVEQATQAIPMERWCGGSRSGSCAHPHH
QVVPFRCLPGEFVSEALLVPEGCRFLHQERMDQCESSTRRHQEAQEACSSQGLILHGSGMLLPCGSDRFRGVEYVCCPPPGTPDPSGTAV
GDPSTRSWPPGSRVEGAEDEEEEESFPQPVDDYFVEPPQAEEEEETVPPPSSHTLAVVGKVTPTPRPTDGVDIYFGMPGEISEHEGFLRA
KMDLEERRMRQINEVMREWAMADNQSKNLPKADRQALNEHFQSILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLAALQADP
PQAERVLLALRRYLRAEQKEQRHTLRHYQHVAAVDPEKAQQMRFQVHTHLQVIEERVNQSLGLLDQNPHLAQELRPQIQELLHSEHLGPS
ELEAPAPGGSSEDKGGLQPPDSKDDTPMTLPKGSTEQDAASPEKEKMNPLEQYERKVNASVPRGFPFHSSEIQRDELAPAGTGVSREAVS

--------------------------------------------------------------

>1763_1763_5_ACTN4-APLP1_ACTN4_chr19_39138547_ENST00000252699_APLP1_chr19_36361798_ENST00000586861_length(amino acids)=607AA_BP=54
MVDYHAANQSYQYGPSSAGNGAGGGGSMGDYMAQEDDWDRDLLLDPAWEKQQRKMYPELQIARVEQATQAIPMERWCGGSRSGSCAHPHH
QVVPFRCLPGEFVSEALLVPEGCRFLHQERMDQCESSTRRHQEAQEACSSQGLILHGSGMLLPCGSDRFRGVEYVCCPPPGTPDPSGTAV
GDPSTRSWPPGSRVEGAEDEEEEESFPQPVDDYFVEPPQAEEEEETVPPPSSHTLAVVGKVTPTPRPTDGVDIYFGMPGEISEHEGFLRA
KMDLEERRMRQINEVMREWAMADNQSKNLPKADRQALNEHFQSILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLAALQADP
PQAERVLLALRRYLRAEQKEQRHTLRHYQHVAAVDPEKAQQMRFQVHTHLQVIEERVNQSLGLLDQNPHLAQELRPQIQELLHSEHLGPS
ELEAPAPGGSSEDKGGLQPPDSKDDTPMTLPKGSTEQDAASPEKEKMNPLEQYERKVNASVPRGFPFHSSEIQRDELAPAGTGVSREAVS

--------------------------------------------------------------

>1763_1763_6_ACTN4-APLP1_ACTN4_chr19_39138547_ENST00000390009_APLP1_chr19_36360569_ENST00000221891_length(amino acids)=656AA_BP=54
MVDYHAANQSYQYGPSSAGNGAGGGGSMGDYMAQEDDWDRDLLLDPAWEKQQRKAPGSAQVAGLCGRLTLHRDLRTGRWEPDPQRSRRCL
RDPQRVLEYCRQMYPELQIARVEQATQAIPMERWCGGSRSGSCAHPHHQVVPFRCLPGEFVSEALLVPEGCRFLHQERMDQCESSTRRHQ
EAQEACSSQGLILHGSGMLLPCGSDRFRGVEYVCCPPPGTPDPSGTAVGDPSTRSWPPGSRVEGAEDEEEEESFPQPVDDYFVEPPQAEE
EEETVPPPSSHTLAVVGKVTPTPRPTDGVDIYFGMPGEISEHEGFLRAKMDLEERRMRQINEVMREWAMADNQSKNLPKADRQALNEHFQ
SILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLAALQADPPQAERVLLALRRYLRAEQKEQRHTLRHYQHVAAVDPEKAQQM
RFQVHTHLQVIEERVNQSLGLLDQNPHLAQELRPQIQELLHSEHLGPSELEAPAPGGSSEDKGGLQPPDSKDADTPMTLPKGSTEQDAAS
PEKEKMNPLEQYERKVNASVPRGFPFHSSEIQRDELAPAGTGVSREAVSGLLIMGAGGGSLIVLSMLLLRRKKPYGAISHGVVEVDPMLT

--------------------------------------------------------------

>1763_1763_7_ACTN4-APLP1_ACTN4_chr19_39138547_ENST00000390009_APLP1_chr19_36360569_ENST00000537454_length(amino acids)=655AA_BP=54
MVDYHAANQSYQYGPSSAGNGAGGGGSMGDYMAQEDDWDRDLLLDPAWEKQQRKAPGSAQVAGLCGRLTLHRDLRTGRWEPDPQRSRRCL
RDPQRVLEYCRQMYPELQIARVEQATQAIPMERWCGGSRSGSCAHPHHQVVPFRCLPGEFVSEALLVPEGCRFLHQERMDQCESSTRRHQ
EAQEACSSQGLILHGSGMLLPCGSDRFRGVEYVCCPPPGTPDPSGTAVGDPSTRSWPPGSRVEGAEDEEEEESFPQPVDDYFVEPPQAEE
EEETVPPPSSHTLAVVGKVTPTPRPTDGVDIYFGMPGEISEHEGFLRAKMDLEERRMRQINEVMREWAMADNQSKNLPKADRQALNEHFQ
SILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLAALQADPPQAERVLLALRRYLRAEQKEQRHTLRHYQHVAAVDPEKAQQM
RFQVHTHLQVIEERVNQSLGLLDQNPHLAQELRPQIQELLHSEHLGPSELEAPAPGGSSEDKGGLQPPDSKDDTPMTLPKGSTEQDAASP
EKEKMNPLEQYERKVNASVPRGFPFHSSEIQRDELAPAGTGVSREAVSGLLIMGAGGGSLIVLSMLLLRRKKPYGAISHGVVEVDPMLTL

--------------------------------------------------------------

>1763_1763_8_ACTN4-APLP1_ACTN4_chr19_39138547_ENST00000390009_APLP1_chr19_36361798_ENST00000221891_length(amino acids)=608AA_BP=54
MVDYHAANQSYQYGPSSAGNGAGGGGSMGDYMAQEDDWDRDLLLDPAWEKQQRKMYPELQIARVEQATQAIPMERWCGGSRSGSCAHPHH
QVVPFRCLPGEFVSEALLVPEGCRFLHQERMDQCESSTRRHQEAQEACSSQGLILHGSGMLLPCGSDRFRGVEYVCCPPPGTPDPSGTAV
GDPSTRSWPPGSRVEGAEDEEEEESFPQPVDDYFVEPPQAEEEEETVPPPSSHTLAVVGKVTPTPRPTDGVDIYFGMPGEISEHEGFLRA
KMDLEERRMRQINEVMREWAMADNQSKNLPKADRQALNEHFQSILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLAALQADP
PQAERVLLALRRYLRAEQKEQRHTLRHYQHVAAVDPEKAQQMRFQVHTHLQVIEERVNQSLGLLDQNPHLAQELRPQIQELLHSEHLGPS
ELEAPAPGGSSEDKGGLQPPDSKDADTPMTLPKGSTEQDAASPEKEKMNPLEQYERKVNASVPRGFPFHSSEIQRDELAPAGTGVSREAV

--------------------------------------------------------------

>1763_1763_9_ACTN4-APLP1_ACTN4_chr19_39138547_ENST00000390009_APLP1_chr19_36361798_ENST00000537454_length(amino acids)=607AA_BP=54
MVDYHAANQSYQYGPSSAGNGAGGGGSMGDYMAQEDDWDRDLLLDPAWEKQQRKMYPELQIARVEQATQAIPMERWCGGSRSGSCAHPHH
QVVPFRCLPGEFVSEALLVPEGCRFLHQERMDQCESSTRRHQEAQEACSSQGLILHGSGMLLPCGSDRFRGVEYVCCPPPGTPDPSGTAV
GDPSTRSWPPGSRVEGAEDEEEEESFPQPVDDYFVEPPQAEEEEETVPPPSSHTLAVVGKVTPTPRPTDGVDIYFGMPGEISEHEGFLRA
KMDLEERRMRQINEVMREWAMADNQSKNLPKADRQALNEHFQSILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLAALQADP
PQAERVLLALRRYLRAEQKEQRHTLRHYQHVAAVDPEKAQQMRFQVHTHLQVIEERVNQSLGLLDQNPHLAQELRPQIQELLHSEHLGPS
ELEAPAPGGSSEDKGGLQPPDSKDDTPMTLPKGSTEQDAASPEKEKMNPLEQYERKVNASVPRGFPFHSSEIQRDELAPAGTGVSREAVS

--------------------------------------------------------------

>1763_1763_10_ACTN4-APLP1_ACTN4_chr19_39138547_ENST00000390009_APLP1_chr19_36361798_ENST00000586861_length(amino acids)=607AA_BP=54
MVDYHAANQSYQYGPSSAGNGAGGGGSMGDYMAQEDDWDRDLLLDPAWEKQQRKMYPELQIARVEQATQAIPMERWCGGSRSGSCAHPHH
QVVPFRCLPGEFVSEALLVPEGCRFLHQERMDQCESSTRRHQEAQEACSSQGLILHGSGMLLPCGSDRFRGVEYVCCPPPGTPDPSGTAV
GDPSTRSWPPGSRVEGAEDEEEEESFPQPVDDYFVEPPQAEEEEETVPPPSSHTLAVVGKVTPTPRPTDGVDIYFGMPGEISEHEGFLRA
KMDLEERRMRQINEVMREWAMADNQSKNLPKADRQALNEHFQSILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLAALQADP
PQAERVLLALRRYLRAEQKEQRHTLRHYQHVAAVDPEKAQQMRFQVHTHLQVIEERVNQSLGLLDQNPHLAQELRPQIQELLHSEHLGPS
ELEAPAPGGSSEDKGGLQPPDSKDDTPMTLPKGSTEQDAASPEKEKMNPLEQYERKVNASVPRGFPFHSSEIQRDELAPAGTGVSREAVS

--------------------------------------------------------------

>1763_1763_11_ACTN4-APLP1_ACTN4_chr19_39138547_ENST00000424234_APLP1_chr19_36360569_ENST00000221891_length(amino acids)=656AA_BP=54
MVDYHAANQSYQYGPSSAGNGAGGGGSMGDYMAQEDDWDRDLLLDPAWEKQQRKAPGSAQVAGLCGRLTLHRDLRTGRWEPDPQRSRRCL
RDPQRVLEYCRQMYPELQIARVEQATQAIPMERWCGGSRSGSCAHPHHQVVPFRCLPGEFVSEALLVPEGCRFLHQERMDQCESSTRRHQ
EAQEACSSQGLILHGSGMLLPCGSDRFRGVEYVCCPPPGTPDPSGTAVGDPSTRSWPPGSRVEGAEDEEEEESFPQPVDDYFVEPPQAEE
EEETVPPPSSHTLAVVGKVTPTPRPTDGVDIYFGMPGEISEHEGFLRAKMDLEERRMRQINEVMREWAMADNQSKNLPKADRQALNEHFQ
SILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLAALQADPPQAERVLLALRRYLRAEQKEQRHTLRHYQHVAAVDPEKAQQM
RFQVHTHLQVIEERVNQSLGLLDQNPHLAQELRPQIQELLHSEHLGPSELEAPAPGGSSEDKGGLQPPDSKDADTPMTLPKGSTEQDAAS
PEKEKMNPLEQYERKVNASVPRGFPFHSSEIQRDELAPAGTGVSREAVSGLLIMGAGGGSLIVLSMLLLRRKKPYGAISHGVVEVDPMLT

--------------------------------------------------------------

>1763_1763_12_ACTN4-APLP1_ACTN4_chr19_39138547_ENST00000424234_APLP1_chr19_36360569_ENST00000537454_length(amino acids)=655AA_BP=54
MVDYHAANQSYQYGPSSAGNGAGGGGSMGDYMAQEDDWDRDLLLDPAWEKQQRKAPGSAQVAGLCGRLTLHRDLRTGRWEPDPQRSRRCL
RDPQRVLEYCRQMYPELQIARVEQATQAIPMERWCGGSRSGSCAHPHHQVVPFRCLPGEFVSEALLVPEGCRFLHQERMDQCESSTRRHQ
EAQEACSSQGLILHGSGMLLPCGSDRFRGVEYVCCPPPGTPDPSGTAVGDPSTRSWPPGSRVEGAEDEEEEESFPQPVDDYFVEPPQAEE
EEETVPPPSSHTLAVVGKVTPTPRPTDGVDIYFGMPGEISEHEGFLRAKMDLEERRMRQINEVMREWAMADNQSKNLPKADRQALNEHFQ
SILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLAALQADPPQAERVLLALRRYLRAEQKEQRHTLRHYQHVAAVDPEKAQQM
RFQVHTHLQVIEERVNQSLGLLDQNPHLAQELRPQIQELLHSEHLGPSELEAPAPGGSSEDKGGLQPPDSKDDTPMTLPKGSTEQDAASP
EKEKMNPLEQYERKVNASVPRGFPFHSSEIQRDELAPAGTGVSREAVSGLLIMGAGGGSLIVLSMLLLRRKKPYGAISHGVVEVDPMLTL

--------------------------------------------------------------

>1763_1763_13_ACTN4-APLP1_ACTN4_chr19_39138547_ENST00000424234_APLP1_chr19_36361798_ENST00000221891_length(amino acids)=608AA_BP=54
MVDYHAANQSYQYGPSSAGNGAGGGGSMGDYMAQEDDWDRDLLLDPAWEKQQRKMYPELQIARVEQATQAIPMERWCGGSRSGSCAHPHH
QVVPFRCLPGEFVSEALLVPEGCRFLHQERMDQCESSTRRHQEAQEACSSQGLILHGSGMLLPCGSDRFRGVEYVCCPPPGTPDPSGTAV
GDPSTRSWPPGSRVEGAEDEEEEESFPQPVDDYFVEPPQAEEEEETVPPPSSHTLAVVGKVTPTPRPTDGVDIYFGMPGEISEHEGFLRA
KMDLEERRMRQINEVMREWAMADNQSKNLPKADRQALNEHFQSILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLAALQADP
PQAERVLLALRRYLRAEQKEQRHTLRHYQHVAAVDPEKAQQMRFQVHTHLQVIEERVNQSLGLLDQNPHLAQELRPQIQELLHSEHLGPS
ELEAPAPGGSSEDKGGLQPPDSKDADTPMTLPKGSTEQDAASPEKEKMNPLEQYERKVNASVPRGFPFHSSEIQRDELAPAGTGVSREAV

--------------------------------------------------------------

>1763_1763_14_ACTN4-APLP1_ACTN4_chr19_39138547_ENST00000424234_APLP1_chr19_36361798_ENST00000537454_length(amino acids)=607AA_BP=54
MVDYHAANQSYQYGPSSAGNGAGGGGSMGDYMAQEDDWDRDLLLDPAWEKQQRKMYPELQIARVEQATQAIPMERWCGGSRSGSCAHPHH
QVVPFRCLPGEFVSEALLVPEGCRFLHQERMDQCESSTRRHQEAQEACSSQGLILHGSGMLLPCGSDRFRGVEYVCCPPPGTPDPSGTAV
GDPSTRSWPPGSRVEGAEDEEEEESFPQPVDDYFVEPPQAEEEEETVPPPSSHTLAVVGKVTPTPRPTDGVDIYFGMPGEISEHEGFLRA
KMDLEERRMRQINEVMREWAMADNQSKNLPKADRQALNEHFQSILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLAALQADP
PQAERVLLALRRYLRAEQKEQRHTLRHYQHVAAVDPEKAQQMRFQVHTHLQVIEERVNQSLGLLDQNPHLAQELRPQIQELLHSEHLGPS
ELEAPAPGGSSEDKGGLQPPDSKDDTPMTLPKGSTEQDAASPEKEKMNPLEQYERKVNASVPRGFPFHSSEIQRDELAPAGTGVSREAVS

--------------------------------------------------------------

>1763_1763_15_ACTN4-APLP1_ACTN4_chr19_39138547_ENST00000424234_APLP1_chr19_36361798_ENST00000586861_length(amino acids)=607AA_BP=54
MVDYHAANQSYQYGPSSAGNGAGGGGSMGDYMAQEDDWDRDLLLDPAWEKQQRKMYPELQIARVEQATQAIPMERWCGGSRSGSCAHPHH
QVVPFRCLPGEFVSEALLVPEGCRFLHQERMDQCESSTRRHQEAQEACSSQGLILHGSGMLLPCGSDRFRGVEYVCCPPPGTPDPSGTAV
GDPSTRSWPPGSRVEGAEDEEEEESFPQPVDDYFVEPPQAEEEEETVPPPSSHTLAVVGKVTPTPRPTDGVDIYFGMPGEISEHEGFLRA
KMDLEERRMRQINEVMREWAMADNQSKNLPKADRQALNEHFQSILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLAALQADP
PQAERVLLALRRYLRAEQKEQRHTLRHYQHVAAVDPEKAQQMRFQVHTHLQVIEERVNQSLGLLDQNPHLAQELRPQIQELLHSEHLGPS
ELEAPAPGGSSEDKGGLQPPDSKDDTPMTLPKGSTEQDAASPEKEKMNPLEQYERKVNASVPRGFPFHSSEIQRDELAPAGTGVSREAVS

--------------------------------------------------------------

Top

Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr19:39138547/chr19:36360569)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
ACTN4 O43707	APLP1 P51693
FUNCTION: F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (Probable). Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation (PubMed:15772161). Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. Links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions (By similarity). May also function as a transcriptional coactivator, stimulating transcription mediated by the nuclear hormone receptors PPARG and RARA (PubMed:22351778). {ECO:0000250\|UniProtKB:P57780, ECO:0000269\|PubMed:15772161, ECO:0000269\|PubMed:22351778, ECO:0000305\|PubMed:9508771}.	FUNCTION: May play a role in postsynaptic function. The C-terminal gamma-secretase processed fragment, ALID1, activates transcription activation through APBB1 (Fe65) binding (By similarity). Couples to JIP signal transduction through C-terminal binding. May interact with cellular G-protein signaling pathways. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I. {ECO:0000250}.; FUNCTION: The gamma-CTF peptide, C30, is a potent enhancer of neuronal apoptosis. {ECO:0000250}.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

ACTN4

chr19:39138547

chr19:36360569

ENST00000252699

19_26

54.0

912.0

Compositional bias

Poly-Gly

Hgene

ACTN4

chr19:39138547

chr19:36360569

ENST00000390009

19_26

54.0

693.0

Compositional bias

Poly-Gly

Hgene

ACTN4

chr19:39138547

chr19:36361798

ENST00000252699

19_26

54.0

912.0

Compositional bias

Poly-Gly

Hgene

ACTN4

chr19:39138547

chr19:36361798

ENST00000390009

19_26

54.0

693.0

Compositional bias

Poly-Gly

Tgene

APLP1

chr19:39138547

chr19:36360569

ENST00000221891

241_247

49.0

652.0

Compositional bias

Note=Poly-Glu

Tgene

APLP1

chr19:39138547

chr19:36360569

ENST00000221891

264_268

49.0

652.0

Compositional bias

Note=Poly-Glu

Tgene

APLP1

chr19:39138547

chr19:36361798

ENST00000221891

241_247

97.0

652.0

Compositional bias

Note=Poly-Glu

Tgene

APLP1

chr19:39138547

chr19:36361798

ENST00000221891

264_268

97.0

652.0

Compositional bias

Note=Poly-Glu

Tgene

APLP1

chr19:39138547

chr19:36360569

ENST00000221891

293_484

49.0

652.0

Domain

Tgene

APLP1

chr19:39138547

chr19:36360569

ENST00000221891

50_212

49.0

652.0

Domain

Tgene

APLP1

chr19:39138547

chr19:36361798

ENST00000221891

293_484

97.0

652.0

Domain

Tgene

APLP1

chr19:39138547

chr19:36360569

ENST00000221891

604_615

49.0

652.0

Motif

Basolateral sorting signal

Tgene

APLP1

chr19:39138547

chr19:36360569

ENST00000221891

640_643

49.0

652.0

Motif

Note=NPXY motif%3B contains endocytosis signal

Tgene

APLP1

chr19:39138547

chr19:36361798

ENST00000221891

604_615

97.0

652.0

Motif

Basolateral sorting signal

Tgene

APLP1

chr19:39138547

chr19:36361798

ENST00000221891

640_643

97.0

652.0

Motif

Note=NPXY motif%3B contains endocytosis signal

Tgene

APLP1

chr19:39138547

chr19:36360569

ENST00000221891

154_212

49.0

652.0

Region

CuBD subdomain

Tgene

APLP1

chr19:39138547

chr19:36360569

ENST00000221891

158_178

49.0

652.0

Region

Copper-binding

Tgene

APLP1

chr19:39138547

chr19:36360569

ENST00000221891

204_211

49.0

652.0

Region

Note=Zinc-binding

Tgene

APLP1

chr19:39138547

chr19:36360569

ENST00000221891

285_305

49.0

652.0

Region

Note=O-glycosylated at three sites

Tgene

APLP1

chr19:39138547

chr19:36360569

ENST00000221891

310_342

49.0

652.0

Region

Heparin-binding

Tgene

APLP1

chr19:39138547

chr19:36360569

ENST00000221891

410_441

49.0

652.0

Region

Heparin-binding

Tgene

APLP1

chr19:39138547

chr19:36360569

ENST00000221891

442_459

49.0

652.0

Region

Collagen-binding

Tgene

APLP1

chr19:39138547

chr19:36360569

ENST00000221891

50_146

49.0

652.0

Region

GFLD subdomain

Tgene

APLP1

chr19:39138547

chr19:36361798

ENST00000221891

154_212

97.0

652.0

Region

CuBD subdomain

Tgene

APLP1

chr19:39138547

chr19:36361798

ENST00000221891

158_178

97.0

652.0

Region

Copper-binding

Tgene

APLP1

chr19:39138547

chr19:36361798

ENST00000221891

204_211

97.0

652.0

Region

Note=Zinc-binding

Tgene

APLP1

chr19:39138547

chr19:36361798

ENST00000221891

285_305

97.0

652.0

Region

Note=O-glycosylated at three sites

Tgene

APLP1

chr19:39138547

chr19:36361798

ENST00000221891

310_342

97.0

652.0

Region

Heparin-binding

Tgene

APLP1

chr19:39138547

chr19:36361798

ENST00000221891

410_441

97.0

652.0

Region

Heparin-binding

Tgene

APLP1

chr19:39138547

chr19:36361798

ENST00000221891

442_459

97.0

652.0

Region

Collagen-binding

Tgene

APLP1

chr19:39138547

chr19:36360569

ENST00000221891

604_650

49.0

652.0

Topological domain

Cytoplasmic

Tgene

APLP1

chr19:39138547

chr19:36361798

ENST00000221891

604_650

97.0

652.0

Topological domain

Cytoplasmic

Tgene

APLP1

chr19:39138547

chr19:36360569

ENST00000221891

581_603

49.0

652.0

Transmembrane

Helical

Tgene

APLP1

chr19:39138547

chr19:36361798

ENST00000221891

581_603

97.0

652.0

Transmembrane

Helical

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

ACTN4

chr19:39138547

chr19:36360569

ENST00000252699

778_789

54.0

912.0

Calcium binding

Hgene

ACTN4

chr19:39138547

chr19:36360569

ENST00000252699

819_830

54.0

912.0

Calcium binding

Hgene

ACTN4

chr19:39138547

chr19:36360569

ENST00000390009

778_789

54.0

693.0

Calcium binding

Hgene

ACTN4

chr19:39138547

chr19:36360569

ENST00000390009

819_830

54.0

693.0

Calcium binding

Hgene

ACTN4

chr19:39138547

chr19:36361798

ENST00000252699

778_789

54.0

912.0

Calcium binding

Hgene

ACTN4

chr19:39138547

chr19:36361798

ENST00000252699

819_830

54.0

912.0

Calcium binding

Hgene

ACTN4

chr19:39138547

chr19:36361798

ENST00000390009

778_789

54.0

693.0

Calcium binding

Hgene

ACTN4

chr19:39138547

chr19:36361798

ENST00000390009

819_830

54.0

693.0

Calcium binding

Hgene

ACTN4

chr19:39138547

chr19:36360569

ENST00000252699

163_269

54.0

912.0

Domain

Calponin-homology (CH) 2

Hgene

ACTN4

chr19:39138547

chr19:36360569

ENST00000252699

50_154

54.0

912.0

Domain

Calponin-homology (CH) 1

Hgene

ACTN4

chr19:39138547

chr19:36360569

ENST00000252699

765_800

54.0

912.0

Domain

EF-hand 1

Hgene

ACTN4

chr19:39138547

chr19:36360569

ENST00000252699

806_841

54.0

912.0

Domain

EF-hand 2

Hgene

ACTN4

chr19:39138547

chr19:36360569

ENST00000390009

163_269

54.0

693.0

Domain

Calponin-homology (CH) 2

Hgene

ACTN4

chr19:39138547

chr19:36360569

ENST00000390009

50_154

54.0

693.0

Domain

Calponin-homology (CH) 1

Hgene

ACTN4

chr19:39138547

chr19:36360569

ENST00000390009

765_800

54.0

693.0

Domain

EF-hand 1

Hgene

ACTN4

chr19:39138547

chr19:36360569

ENST00000390009

806_841

54.0

693.0

Domain

EF-hand 2

Hgene

ACTN4

chr19:39138547

chr19:36361798

ENST00000252699

163_269

54.0

912.0

Domain

Calponin-homology (CH) 2

Hgene

ACTN4

chr19:39138547

chr19:36361798

ENST00000252699

50_154

54.0

912.0

Domain

Calponin-homology (CH) 1

Hgene

ACTN4

chr19:39138547

chr19:36361798

ENST00000252699

765_800

54.0

912.0

Domain

EF-hand 1

Hgene

ACTN4

chr19:39138547

chr19:36361798

ENST00000252699

806_841

54.0

912.0

Domain

EF-hand 2

Hgene

ACTN4

chr19:39138547

chr19:36361798

ENST00000390009

163_269

54.0

693.0

Domain

Calponin-homology (CH) 2

Hgene

ACTN4

chr19:39138547

chr19:36361798

ENST00000390009

50_154

54.0

693.0

Domain

Calponin-homology (CH) 1

Hgene

ACTN4

chr19:39138547

chr19:36361798

ENST00000390009

765_800

54.0

693.0

Domain

EF-hand 1

Hgene

ACTN4

chr19:39138547

chr19:36361798

ENST00000390009

806_841

54.0

693.0

Domain

EF-hand 2

Hgene

ACTN4

chr19:39138547

chr19:36360569

ENST00000252699

84_88

54.0

912.0

Motif

LXXLL motif

Hgene

ACTN4

chr19:39138547

chr19:36360569

ENST00000390009

84_88

54.0

693.0

Motif

LXXLL motif

Hgene

ACTN4

chr19:39138547

chr19:36361798

ENST00000252699

84_88

54.0

912.0

Motif

LXXLL motif

Hgene

ACTN4

chr19:39138547

chr19:36361798

ENST00000390009

84_88

54.0

693.0

Motif

LXXLL motif

Hgene

ACTN4

chr19:39138547

chr19:36360569

ENST00000252699

177_192

54.0

912.0

Region

Polyphosphoinositide (PIP2)-binding

Hgene

ACTN4

chr19:39138547

chr19:36360569

ENST00000252699

1_269

54.0

912.0

Region

Note=Actin-binding

Hgene

ACTN4

chr19:39138547

chr19:36360569

ENST00000390009

177_192

54.0

693.0

Region

Polyphosphoinositide (PIP2)-binding

Hgene

ACTN4

chr19:39138547

chr19:36360569

ENST00000390009

1_269

54.0

693.0

Region

Note=Actin-binding

Hgene

ACTN4

chr19:39138547

chr19:36361798

ENST00000252699

177_192

54.0

912.0

Region

Polyphosphoinositide (PIP2)-binding

Hgene

ACTN4

chr19:39138547

chr19:36361798

ENST00000252699

1_269

54.0

912.0

Region

Note=Actin-binding

Hgene

ACTN4

chr19:39138547

chr19:36361798

ENST00000390009

177_192

54.0

693.0

Region

Polyphosphoinositide (PIP2)-binding

Hgene

ACTN4

chr19:39138547

chr19:36361798

ENST00000390009

1_269

54.0

693.0

Region

Note=Actin-binding

Hgene

ACTN4

chr19:39138547

chr19:36360569

ENST00000252699

293_403

54.0

912.0

Repeat

Spectrin 1

Hgene

ACTN4

chr19:39138547

chr19:36360569

ENST00000252699

413_518

54.0

912.0

Repeat

Spectrin 2

Hgene

ACTN4

chr19:39138547

chr19:36360569

ENST00000252699

528_639

54.0

912.0

Repeat

Spectrin 3

Hgene

ACTN4

chr19:39138547

chr19:36360569

ENST00000252699

649_752

54.0

912.0

Repeat

Spectrin 4

Hgene

ACTN4

chr19:39138547

chr19:36360569

ENST00000390009

293_403

54.0

693.0

Repeat

Spectrin 1

Hgene

ACTN4

chr19:39138547

chr19:36360569

ENST00000390009

413_518

54.0

693.0

Repeat

Spectrin 2

Hgene

ACTN4

chr19:39138547

chr19:36360569

ENST00000390009

528_639

54.0

693.0

Repeat

Spectrin 3

Hgene

ACTN4

chr19:39138547

chr19:36360569

ENST00000390009

649_752

54.0

693.0

Repeat

Spectrin 4

Hgene

ACTN4

chr19:39138547

chr19:36361798

ENST00000252699

293_403

54.0

912.0

Repeat

Spectrin 1

Hgene

ACTN4

chr19:39138547

chr19:36361798

ENST00000252699

413_518

54.0

912.0

Repeat

Spectrin 2

Hgene

ACTN4

chr19:39138547

chr19:36361798

ENST00000252699

528_639

54.0

912.0

Repeat

Spectrin 3

Hgene

ACTN4

chr19:39138547

chr19:36361798

ENST00000252699

649_752

54.0

912.0

Repeat

Spectrin 4

Hgene

ACTN4

chr19:39138547

chr19:36361798

ENST00000390009

293_403

54.0

693.0

Repeat

Spectrin 1

Hgene

ACTN4

chr19:39138547

chr19:36361798

ENST00000390009

413_518

54.0

693.0

Repeat

Spectrin 2

Hgene

ACTN4

chr19:39138547

chr19:36361798

ENST00000390009

528_639

54.0

693.0

Repeat

Spectrin 3

Hgene

ACTN4

chr19:39138547

chr19:36361798

ENST00000390009

649_752

54.0

693.0

Repeat

Spectrin 4

Tgene

APLP1

chr19:39138547

chr19:36361798

ENST00000221891

50_212

97.0

652.0

Domain

Tgene

APLP1

chr19:39138547

chr19:36361798

ENST00000221891

50_146

97.0

652.0

Region

GFLD subdomain

Tgene

APLP1

chr19:39138547

chr19:36360569

ENST00000221891

39_580

49.0

652.0

Topological domain

Extracellular

Tgene

APLP1

chr19:39138547

chr19:36361798

ENST00000221891

39_580

97.0

652.0

Topological domain

Extracellular

Top

Fusion Protein Structures

PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.

Fusion protein PDB link (fusion AA seq ID in FusionPDB)

Hgene

Hchr

Hbp

Hstrand

Tgene

Tchr

Tbp

Tstrand

AA seq

Len(AA seq)

Top

pLDDT score distribution

pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

ACTN4_pLDDT.png

APLP1_pLDDT.png

pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

Top

Ramachandran Plot of Fusion Protein Structure

Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.

Fusion AA seq ID in FusionPDB and their Ramachandran plots

Top

Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

ACTN4

MYOZ2, MYOZ1, PDLIM1, USP6NL, P2rx7, SLC9A3R2, COL17A1, TRIM3, MAGI1, GSN, Lrrc7, CAMK2A, CAMK2B, INO80, UBTF, STAT1, HDAC7, MEF2A, UCHL5, TJP1, ATXN7, HDAC5, VDR, ESR1, PSMA3, ELAVL1, SIRT7, SLC2A4, ISG15, COIL, SACS, HGS, UBASH3B, SHC1, NOS3, ACTN2, ACTN1, FMNL1, TRAF3IP1, FN1, VCAM1, Fcho2, CDH1, ITGA4, SVIL, CD81, IGSF8, ICAM1, TXN, RPS27, POT1, KPNA2, MAPK7, CDKN2A, VCP, SF3A3, GNB2, HUWE1, ACTN3, BMP7, ALK, CUL7, OBSL1, CCDC8, EGFR, LUZP4, RARA, HSPB1, AHNAK, ALDOA, ALDOC, IDH3A, MYH15, MYH2, MYH7B, NCAPG2, PSMA6, PSMB5, PUS7, TPM2, TSG101, CKB, CYC1, ECSIT, MCFD2, PSMB8, TPM1, TPM3, TPM4, NTRK1, CAPZA2, DBN1, FLNA, MYH9, MYO1C, PPP1CB, IQGAP1, PDLIM7, SYNPO, MAPRE1, SIN3B, MYEF2, LIMA1, ANLN, MYO5C, MYO19, MYO18A, Actb, Flot2, Myh9, Myo1c, Tpm1, Coro1c, Tmod3, Lima1, Ncbp2, Calml3, Myh10, Flnb, CTNNB1, MCM2, NFYA, CDC73, LCMT2, MEOX2, SORBS2, DDIAS, SSH1, act1, SLC9A1, NCL, DLD, DNM1L, PDHA1, SOD1, TRIM25, TES, CFTR, PPM1D, HDAC4, UBE2M, RAD18, PRPF8, TNIP2, RNF4, TRIM23, AGR2, ACTA1, MYC, CDK9, KIAA1429, ATG16L1, ACTC1, USP14, HDAC2, GBF1, AGRN, DYNC1LI1, BMH1, BMH2, ATXN3, BRF1, CYB5B, GBAS, HADHB, SYNJ2BP, VDAC3, RELA, PRMT5, MDP1, GRIN1, RGS9, BIRC3, SOX2, LNX1, MICALL2, C14orf119, PLEKHA4, PINK1, FANCD2, SH2D3C, SRC, TTN, NEK4, CIT, CHMP4B, CHMP4C, PTGER4, ECT2, KIF14, KIF20A, PRC1, PRNP, MKI67, HNRNPH1, NMRAL1, NUPR1, TRIM21, Apc2, RBM39, LGALS9, YWHAE, EIF3F, INSIG1, INSIG2, RIN3, CANX, GGH, ERP44, ACO2, KDM4C, AHCY, SHMT2, IDH2, SMCHD1, AR, TP53, ACTB, ANKFY1, EZR, RDX, VASP, ZYX, TRIM37, HTRA4, NUDCD2, BGLT3, BTF3, SEPT9, UBR5, LHPP, TFRC, CTSB, CTSS, DPP4, ORF3a, ORF8, NRP1, TMPRSS2, TOP3B, CTSL, FURIN, IFITM1, ANPEP, BSG, IFITM3, TMPRSS11B, CLEC4D, ACE2, TMPRSS4, CLEC4E, nsp2, ORF10, FGD5, TMEM106B, PDE4B, PER2, OTUD3, SIK2,

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
ACTN4
APLP1

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner	Gene	Hbp	Tbp	ENST	Strand	BPexon	TotalExon	Protein feature loci	*BPloci	TotalLen	Interaction lost with
Hgene	ACTN4	chr19:39138547	chr19:36360569	ENST00000252699	+	1	21	108_126	54.0	912.0	VCL
Hgene	ACTN4	chr19:39138547	chr19:36360569	ENST00000252699	+	1	21	40_61	54.0	912.0	VCL
Hgene	ACTN4	chr19:39138547	chr19:36360569	ENST00000390009	+	1	14	108_126	54.0	693.0	VCL
Hgene	ACTN4	chr19:39138547	chr19:36360569	ENST00000390009	+	1	14	40_61	54.0	693.0	VCL
Hgene	ACTN4	chr19:39138547	chr19:36361798	ENST00000252699	+	1	21	108_126	54.0	912.0	VCL
Hgene	ACTN4	chr19:39138547	chr19:36361798	ENST00000252699	+	1	21	40_61	54.0	912.0	VCL
Hgene	ACTN4	chr19:39138547	chr19:36361798	ENST00000390009	+	1	14	108_126	54.0	693.0	VCL
Hgene	ACTN4	chr19:39138547	chr19:36361798	ENST00000390009	+	1	14	40_61	54.0	693.0	VCL

Top

Related Drugs to ACTN4-APLP1

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

Top

Related Diseases to ACTN4-APLP1

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner	Gene	Disease ID	Disease name	# pubmeds	Source
Hgene	ACTN4	C4551527	Focal segmental glomerulosclerosis 1	9	GENOMICS_ENGLAND;UNIPROT
Hgene	ACTN4	C0007097	Carcinoma	1	CTD_human
Hgene	ACTN4	C0019193	Hepatitis, Toxic	1	CTD_human
Hgene	ACTN4	C0024667	Animal Mammary Neoplasms	1	CTD_human
Hgene	ACTN4	C0024668	Mammary Neoplasms, Experimental	1	CTD_human
Hgene	ACTN4	C0205696	Anaplastic carcinoma	1	CTD_human
Hgene	ACTN4	C0205697	Carcinoma, Spindle-Cell	1	CTD_human
Hgene	ACTN4	C0205698	Undifferentiated carcinoma	1	CTD_human
Hgene	ACTN4	C0205699	Carcinomatosis	1	CTD_human
Hgene	ACTN4	C0860207	Drug-Induced Liver Disease	1	CTD_human
Hgene	ACTN4	C1257925	Mammary Carcinoma, Animal	1	CTD_human
Hgene	ACTN4	C1262760	Hepatitis, Drug-Induced	1	CTD_human
Hgene	ACTN4	C1868672	NEPHROTIC SYNDROME, STEROID-RESISTANT, AUTOSOMAL RECESSIVE	1	ORPHANET
Hgene	ACTN4	C3658290	Drug-Induced Acute Liver Injury	1	CTD_human
Hgene	ACTN4	C4277682	Chemical and Drug Induced Liver Injury	1	CTD_human
Hgene	ACTN4	C4279912	Chemically-Induced Liver Toxicity	1	CTD_human