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level3

	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein Structure
	pLDDT scores
	Ramachandran Plot of Fusion Protein Structure
	Potential Active Site Information
	Potentially Interacting Small Molecules through Virtual Screening
	Biochemical Features of Small Molecules with ADME
	Drug Toxicity Information
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:DDX5-ETV4

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: DDX5-ETV4
	FusionPDB ID: 22079
	FusionGDB2.0 ID: 22079
		Hgene	Tgene
	Gene symbol	DDX5	ETV4
	Gene ID	1655	2118
	Gene name	DEAD-box helicase 5	ETS variant transcription factor 4
	Synonyms	G17P1\|HLR1\|HUMP68\|p68	E1A-F\|E1AF\|PEA3\|PEAS3
	Cytomap	17q23.3	17q21.31
	Type of gene	protein-coding	protein-coding
	Description	probable ATP-dependent RNA helicase DDX5ATP-dependent RNA helicase DDX5DEAD (Asp-Glu-Ala-Asp) box helicase 5DEAD (Asp-Glu-Ala-Asp) box polypeptide 5DEAD box protein 5DEAD box-5DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68kD)RNA h	ETS translocation variant 4ETS variant 4EWS protein/E1A enhancer binding protein chimeraadenovirus E1A enhancer-binding proteinets variant gene 4 (E1A enhancer-binding protein, E1AF)polyomavirus enhancer activator 3 homolog
	Modification date	20200313	20200313
	UniProtAcc	Q5T1V6	P43268
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000225792, ENST00000578804, ENST00000450599, ENST00000580026,	ENST00000586826, ENST00000319349, ENST00000393664, ENST00000538265, ENST00000545089, ENST00000545954, ENST00000591713,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	33 X 25 X 10=8250	14 X 28 X 6=2352
	# samples	27	24
	** MAII score	log2(27/8250*10)=-4.93336280696971 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(24/2352*10)=-3.29278174922785 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)	PubMed: DDX5 [Title/Abstract] AND ETV4 [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	DDX5(62502407)-ETV4(41605887), # samples:1 DDX5(62500340)-ETV4(41613848), # samples:1
Anticipated loss of major functional domain due to fusion event.	DDX5-ETV4 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. DDX5-ETV4 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. DDX5-ETV4 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. DDX5-ETV4 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	DDX5	GO:0000122	negative regulation of transcription by RNA polymerase II	15298701
Hgene	DDX5	GO:0000381	regulation of alternative mRNA splicing, via spliceosome	21343338
Hgene	DDX5	GO:0000956	nuclear-transcribed mRNA catabolic process	23788676
Tgene	ETV4	GO:0045944	positive regulation of transcription by RNA polymerase II	24983502

Fusion gene breakpoints across DDX5 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across ETV4 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	prostate cancer	EU599085	DDX5	chr17	62502407		ETV4	chr17	41605887
ChimerKB3	.	.	DDX5	chr17	62500339	-	ETV4	chr17	41613847	-
ChimerKB4	.	.	DDX5	chr17	62500436	-	ETV4	chr17	62500436	-
ChiTaRS5.0	N/A	EU599085	DDX5	chr17	62500340	-	ETV4	chr17	41613848	-

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000225792	DDX5	chr17	62500339	-	ENST00000319349	ETV4	chr17	41613847	-	2637	709	213	1961	582
ENST00000225792	DDX5	chr17	62500339	-	ENST00000393664	ETV4	chr17	41613847	-	2635	709	213	1961	582
ENST00000225792	DDX5	chr17	62500339	-	ENST00000538265	ETV4	chr17	41613847	-	2552	709	213	1961	582
ENST00000225792	DDX5	chr17	62500339	-	ENST00000545954	ETV4	chr17	41613847	-	2203	709	213	1961	582
ENST00000225792	DDX5	chr17	62500339	-	ENST00000545089	ETV4	chr17	41613847	-	2039	709	213	1799	528
ENST00000225792	DDX5	chr17	62500339	-	ENST00000591713	ETV4	chr17	41613847	-	2144	709	213	1961	582
ENST00000578804	DDX5	chr17	62500339	-	ENST00000319349	ETV4	chr17	41613847	-	2402	474	80	1726	548
ENST00000578804	DDX5	chr17	62500339	-	ENST00000393664	ETV4	chr17	41613847	-	2400	474	80	1726	548
ENST00000578804	DDX5	chr17	62500339	-	ENST00000538265	ETV4	chr17	41613847	-	2317	474	80	1726	548
ENST00000578804	DDX5	chr17	62500339	-	ENST00000545954	ETV4	chr17	41613847	-	1968	474	80	1726	548
ENST00000578804	DDX5	chr17	62500339	-	ENST00000545089	ETV4	chr17	41613847	-	1804	474	80	1564	494
ENST00000578804	DDX5	chr17	62500339	-	ENST00000591713	ETV4	chr17	41613847	-	1909	474	80	1726	548
ENST00000225792	DDX5	chr17	62500340	-	ENST00000319349	ETV4	chr17	41613848	-	2637	709	213	1961	582
ENST00000225792	DDX5	chr17	62500340	-	ENST00000393664	ETV4	chr17	41613848	-	2635	709	213	1961	582
ENST00000225792	DDX5	chr17	62500340	-	ENST00000538265	ETV4	chr17	41613848	-	2552	709	213	1961	582
ENST00000225792	DDX5	chr17	62500340	-	ENST00000545954	ETV4	chr17	41613848	-	2203	709	213	1961	582
ENST00000225792	DDX5	chr17	62500340	-	ENST00000545089	ETV4	chr17	41613848	-	2039	709	213	1799	528
ENST00000225792	DDX5	chr17	62500340	-	ENST00000591713	ETV4	chr17	41613848	-	2144	709	213	1961	582
ENST00000578804	DDX5	chr17	62500340	-	ENST00000319349	ETV4	chr17	41613848	-	2402	474	80	1726	548
ENST00000578804	DDX5	chr17	62500340	-	ENST00000393664	ETV4	chr17	41613848	-	2400	474	80	1726	548
ENST00000578804	DDX5	chr17	62500340	-	ENST00000538265	ETV4	chr17	41613848	-	2317	474	80	1726	548
ENST00000578804	DDX5	chr17	62500340	-	ENST00000545954	ETV4	chr17	41613848	-	1968	474	80	1726	548
ENST00000578804	DDX5	chr17	62500340	-	ENST00000545089	ETV4	chr17	41613848	-	1804	474	80	1564	494
ENST00000578804	DDX5	chr17	62500340	-	ENST00000591713	ETV4	chr17	41613848	-	1909	474	80	1726	548

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000225792	ENST00000319349	DDX5	chr17	62500340	-	ETV4	chr17	41613848	-	0.0042093	0.9957908
ENST00000225792	ENST00000393664	DDX5	chr17	62500340	-	ETV4	chr17	41613848	-	0.004237094	0.9957628
ENST00000225792	ENST00000538265	DDX5	chr17	62500340	-	ETV4	chr17	41613848	-	0.004445863	0.99555415
ENST00000225792	ENST00000545954	DDX5	chr17	62500340	-	ETV4	chr17	41613848	-	0.005520404	0.99447954
ENST00000225792	ENST00000545089	DDX5	chr17	62500340	-	ETV4	chr17	41613848	-	0.004925336	0.9950747
ENST00000225792	ENST00000591713	DDX5	chr17	62500340	-	ETV4	chr17	41613848	-	0.005385352	0.99461466
ENST00000578804	ENST00000319349	DDX5	chr17	62500340	-	ETV4	chr17	41613848	-	0.003285883	0.9967141
ENST00000578804	ENST00000393664	DDX5	chr17	62500340	-	ETV4	chr17	41613848	-	0.00331099	0.996689
ENST00000578804	ENST00000538265	DDX5	chr17	62500340	-	ETV4	chr17	41613848	-	0.003388448	0.99661154
ENST00000578804	ENST00000545954	DDX5	chr17	62500340	-	ETV4	chr17	41613848	-	0.00368469	0.9963153
ENST00000578804	ENST00000545089	DDX5	chr17	62500340	-	ETV4	chr17	41613848	-	0.003218035	0.99678195
ENST00000578804	ENST00000591713	DDX5	chr17	62500340	-	ETV4	chr17	41613848	-	0.00359487	0.9964051

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>22079_22079_1_DDX5-ETV4_DDX5_chr17_62500339_ENST00000225792_ETV4_chr17_41613847_ENST00000319349_length(amino acids)=582AA_BP=165
MPESGRHLIHFYRSLVVQLRLVSSVSFLRCRPRKASPSSRPFPATCRTLFYILRSPPTATIDAMSGYSSDRDRGRDRGFGAPRFGGSRAG
PLSGKKFGNPGEKLVKKKWNLDELPKFEKNFYQEHPDLARRTAQEVETYRRSKEITVRGHNCPKPVLNFYEANFPAQVPDSDEQFVPDFH
SENLAFHSPTTRIKKEPQSPRTDPALSCSRKPPLPYHHGEQCLYSSAYDPPRQIAIKSPAPGALGQSPLQPFPRAEQRNFLRSSGTSQPH
PGHGYLGEHSSVFQQPLDICHSFTSQGGGREPLPAPYQHQLSEPCPPYPQQSFKQEYHDPLYEQAGQPAVDQGGVNGHRYPGAGVVIKQE
QTDFAYDSDVTGCASMYLHTEGFSGPSPGDGAMGYGYEKPLRPFPDDVCVVPEKFEGDIKQEGVGAFREGPPYQRRGALQLWQFLVALLD
DPTNAHFIAWTGRGMEFKLIEPEEVARLWGIQKNRPAMNYDKLSRSLRYYYEKGIMQKVAGERYVYKFVCEPEALFSLAFPDNQRPALKA

--------------------------------------------------------------

>22079_22079_2_DDX5-ETV4_DDX5_chr17_62500339_ENST00000225792_ETV4_chr17_41613847_ENST00000393664_length(amino acids)=582AA_BP=165
MPESGRHLIHFYRSLVVQLRLVSSVSFLRCRPRKASPSSRPFPATCRTLFYILRSPPTATIDAMSGYSSDRDRGRDRGFGAPRFGGSRAG
PLSGKKFGNPGEKLVKKKWNLDELPKFEKNFYQEHPDLARRTAQEVETYRRSKEITVRGHNCPKPVLNFYEANFPAQVPDSDEQFVPDFH
SENLAFHSPTTRIKKEPQSPRTDPALSCSRKPPLPYHHGEQCLYSSAYDPPRQIAIKSPAPGALGQSPLQPFPRAEQRNFLRSSGTSQPH
PGHGYLGEHSSVFQQPLDICHSFTSQGGGREPLPAPYQHQLSEPCPPYPQQSFKQEYHDPLYEQAGQPAVDQGGVNGHRYPGAGVVIKQE
QTDFAYDSDVTGCASMYLHTEGFSGPSPGDGAMGYGYEKPLRPFPDDVCVVPEKFEGDIKQEGVGAFREGPPYQRRGALQLWQFLVALLD
DPTNAHFIAWTGRGMEFKLIEPEEVARLWGIQKNRPAMNYDKLSRSLRYYYEKGIMQKVAGERYVYKFVCEPEALFSLAFPDNQRPALKA

--------------------------------------------------------------

>22079_22079_3_DDX5-ETV4_DDX5_chr17_62500339_ENST00000225792_ETV4_chr17_41613847_ENST00000538265_length(amino acids)=582AA_BP=165
MPESGRHLIHFYRSLVVQLRLVSSVSFLRCRPRKASPSSRPFPATCRTLFYILRSPPTATIDAMSGYSSDRDRGRDRGFGAPRFGGSRAG
PLSGKKFGNPGEKLVKKKWNLDELPKFEKNFYQEHPDLARRTAQEVETYRRSKEITVRGHNCPKPVLNFYEANFPAQVPDSDEQFVPDFH
SENLAFHSPTTRIKKEPQSPRTDPALSCSRKPPLPYHHGEQCLYSSAYDPPRQIAIKSPAPGALGQSPLQPFPRAEQRNFLRSSGTSQPH
PGHGYLGEHSSVFQQPLDICHSFTSQGGGREPLPAPYQHQLSEPCPPYPQQSFKQEYHDPLYEQAGQPAVDQGGVNGHRYPGAGVVIKQE
QTDFAYDSDVTGCASMYLHTEGFSGPSPGDGAMGYGYEKPLRPFPDDVCVVPEKFEGDIKQEGVGAFREGPPYQRRGALQLWQFLVALLD
DPTNAHFIAWTGRGMEFKLIEPEEVARLWGIQKNRPAMNYDKLSRSLRYYYEKGIMQKVAGERYVYKFVCEPEALFSLAFPDNQRPALKA

--------------------------------------------------------------

>22079_22079_4_DDX5-ETV4_DDX5_chr17_62500339_ENST00000225792_ETV4_chr17_41613847_ENST00000545089_length(amino acids)=528AA_BP=165
MPESGRHLIHFYRSLVVQLRLVSSVSFLRCRPRKASPSSRPFPATCRTLFYILRSPPTATIDAMSGYSSDRDRGRDRGFGAPRFGGSRAG
PLSGKKFGNPGEKLVKKKWNLDELPKFEKNFYQEHPDLARRTAQEVETYRRSKEITVRGHNCPKPVLNFYEANFPAQVPDSDEQFVPDFH
SENLAFHSPTTRIKKEPQSPRTDPALSCSRKPPLPYHHGEQCLYSSSVFQQPLDICHSFTSQGGGREPLPAPYQHQLSEPCPPYPQQSFK
QEYHDPLYEQAGQPAVDQGGVNGHRYPGAGVVIKQEQTDFAYDSDVTGCASMYLHTEGFSGPSPGDGAMGYGYEKPLRPFPDDVCVVPEK
FEGDIKQEGVGAFREGPPYQRRGALQLWQFLVALLDDPTNAHFIAWTGRGMEFKLIEPEEVARLWGIQKNRPAMNYDKLSRSLRYYYEKG

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>22079_22079_5_DDX5-ETV4_DDX5_chr17_62500339_ENST00000225792_ETV4_chr17_41613847_ENST00000545954_length(amino acids)=582AA_BP=165
MPESGRHLIHFYRSLVVQLRLVSSVSFLRCRPRKASPSSRPFPATCRTLFYILRSPPTATIDAMSGYSSDRDRGRDRGFGAPRFGGSRAG
PLSGKKFGNPGEKLVKKKWNLDELPKFEKNFYQEHPDLARRTAQEVETYRRSKEITVRGHNCPKPVLNFYEANFPAQVPDSDEQFVPDFH
SENLAFHSPTTRIKKEPQSPRTDPALSCSRKPPLPYHHGEQCLYSSAYDPPRQIAIKSPAPGALGQSPLQPFPRAEQRNFLRSSGTSQPH
PGHGYLGEHSSVFQQPLDICHSFTSQGGGREPLPAPYQHQLSEPCPPYPQQSFKQEYHDPLYEQAGQPAVDQGGVNGHRYPGAGVVIKQE
QTDFAYDSDVTGCASMYLHTEGFSGPSPGDGAMGYGYEKPLRPFPDDVCVVPEKFEGDIKQEGVGAFREGPPYQRRGALQLWQFLVALLD
DPTNAHFIAWTGRGMEFKLIEPEEVARLWGIQKNRPAMNYDKLSRSLRYYYEKGIMQKVAGERYVYKFVCEPEALFSLAFPDNQRPALKA

--------------------------------------------------------------

>22079_22079_6_DDX5-ETV4_DDX5_chr17_62500339_ENST00000225792_ETV4_chr17_41613847_ENST00000591713_length(amino acids)=582AA_BP=165
MPESGRHLIHFYRSLVVQLRLVSSVSFLRCRPRKASPSSRPFPATCRTLFYILRSPPTATIDAMSGYSSDRDRGRDRGFGAPRFGGSRAG
PLSGKKFGNPGEKLVKKKWNLDELPKFEKNFYQEHPDLARRTAQEVETYRRSKEITVRGHNCPKPVLNFYEANFPAQVPDSDEQFVPDFH
SENLAFHSPTTRIKKEPQSPRTDPALSCSRKPPLPYHHGEQCLYSSAYDPPRQIAIKSPAPGALGQSPLQPFPRAEQRNFLRSSGTSQPH
PGHGYLGEHSSVFQQPLDICHSFTSQGGGREPLPAPYQHQLSEPCPPYPQQSFKQEYHDPLYEQAGQPAVDQGGVNGHRYPGAGVVIKQE
QTDFAYDSDVTGCASMYLHTEGFSGPSPGDGAMGYGYEKPLRPFPDDVCVVPEKFEGDIKQEGVGAFREGPPYQRRGALQLWQFLVALLD
DPTNAHFIAWTGRGMEFKLIEPEEVARLWGIQKNRPAMNYDKLSRSLRYYYEKGIMQKVAGERYVYKFVCEPEALFSLAFPDNQRPALKA

--------------------------------------------------------------

>22079_22079_7_DDX5-ETV4_DDX5_chr17_62500339_ENST00000578804_ETV4_chr17_41613847_ENST00000319349_length(amino acids)=548AA_BP=131
MLFVWGLGSCRGGCHLVAVEGVERERIVRMKAGRFEADEGTSVRFGAPRFGGSRAGPLSGKKFGNPGEKLVKKKWNLDELPKFEKNFYQE
HPDLARRTAQEVETYRRSKEITVRGHNCPKPVLNFYEANFPAQVPDSDEQFVPDFHSENLAFHSPTTRIKKEPQSPRTDPALSCSRKPPL
PYHHGEQCLYSSAYDPPRQIAIKSPAPGALGQSPLQPFPRAEQRNFLRSSGTSQPHPGHGYLGEHSSVFQQPLDICHSFTSQGGGREPLP
APYQHQLSEPCPPYPQQSFKQEYHDPLYEQAGQPAVDQGGVNGHRYPGAGVVIKQEQTDFAYDSDVTGCASMYLHTEGFSGPSPGDGAMG
YGYEKPLRPFPDDVCVVPEKFEGDIKQEGVGAFREGPPYQRRGALQLWQFLVALLDDPTNAHFIAWTGRGMEFKLIEPEEVARLWGIQKN
RPAMNYDKLSRSLRYYYEKGIMQKVAGERYVYKFVCEPEALFSLAFPDNQRPALKAEFDRPVSEEDTVPLSHLDESPAYLPELAGPAQPF

--------------------------------------------------------------

>22079_22079_8_DDX5-ETV4_DDX5_chr17_62500339_ENST00000578804_ETV4_chr17_41613847_ENST00000393664_length(amino acids)=548AA_BP=131
MLFVWGLGSCRGGCHLVAVEGVERERIVRMKAGRFEADEGTSVRFGAPRFGGSRAGPLSGKKFGNPGEKLVKKKWNLDELPKFEKNFYQE
HPDLARRTAQEVETYRRSKEITVRGHNCPKPVLNFYEANFPAQVPDSDEQFVPDFHSENLAFHSPTTRIKKEPQSPRTDPALSCSRKPPL
PYHHGEQCLYSSAYDPPRQIAIKSPAPGALGQSPLQPFPRAEQRNFLRSSGTSQPHPGHGYLGEHSSVFQQPLDICHSFTSQGGGREPLP
APYQHQLSEPCPPYPQQSFKQEYHDPLYEQAGQPAVDQGGVNGHRYPGAGVVIKQEQTDFAYDSDVTGCASMYLHTEGFSGPSPGDGAMG
YGYEKPLRPFPDDVCVVPEKFEGDIKQEGVGAFREGPPYQRRGALQLWQFLVALLDDPTNAHFIAWTGRGMEFKLIEPEEVARLWGIQKN
RPAMNYDKLSRSLRYYYEKGIMQKVAGERYVYKFVCEPEALFSLAFPDNQRPALKAEFDRPVSEEDTVPLSHLDESPAYLPELAGPAQPF

--------------------------------------------------------------

>22079_22079_9_DDX5-ETV4_DDX5_chr17_62500339_ENST00000578804_ETV4_chr17_41613847_ENST00000538265_length(amino acids)=548AA_BP=131
MLFVWGLGSCRGGCHLVAVEGVERERIVRMKAGRFEADEGTSVRFGAPRFGGSRAGPLSGKKFGNPGEKLVKKKWNLDELPKFEKNFYQE
HPDLARRTAQEVETYRRSKEITVRGHNCPKPVLNFYEANFPAQVPDSDEQFVPDFHSENLAFHSPTTRIKKEPQSPRTDPALSCSRKPPL
PYHHGEQCLYSSAYDPPRQIAIKSPAPGALGQSPLQPFPRAEQRNFLRSSGTSQPHPGHGYLGEHSSVFQQPLDICHSFTSQGGGREPLP
APYQHQLSEPCPPYPQQSFKQEYHDPLYEQAGQPAVDQGGVNGHRYPGAGVVIKQEQTDFAYDSDVTGCASMYLHTEGFSGPSPGDGAMG
YGYEKPLRPFPDDVCVVPEKFEGDIKQEGVGAFREGPPYQRRGALQLWQFLVALLDDPTNAHFIAWTGRGMEFKLIEPEEVARLWGIQKN
RPAMNYDKLSRSLRYYYEKGIMQKVAGERYVYKFVCEPEALFSLAFPDNQRPALKAEFDRPVSEEDTVPLSHLDESPAYLPELAGPAQPF

--------------------------------------------------------------

>22079_22079_10_DDX5-ETV4_DDX5_chr17_62500339_ENST00000578804_ETV4_chr17_41613847_ENST00000545089_length(amino acids)=494AA_BP=131
MLFVWGLGSCRGGCHLVAVEGVERERIVRMKAGRFEADEGTSVRFGAPRFGGSRAGPLSGKKFGNPGEKLVKKKWNLDELPKFEKNFYQE
HPDLARRTAQEVETYRRSKEITVRGHNCPKPVLNFYEANFPAQVPDSDEQFVPDFHSENLAFHSPTTRIKKEPQSPRTDPALSCSRKPPL
PYHHGEQCLYSSSVFQQPLDICHSFTSQGGGREPLPAPYQHQLSEPCPPYPQQSFKQEYHDPLYEQAGQPAVDQGGVNGHRYPGAGVVIK
QEQTDFAYDSDVTGCASMYLHTEGFSGPSPGDGAMGYGYEKPLRPFPDDVCVVPEKFEGDIKQEGVGAFREGPPYQRRGALQLWQFLVAL
LDDPTNAHFIAWTGRGMEFKLIEPEEVARLWGIQKNRPAMNYDKLSRSLRYYYEKGIMQKVAGERYVYKFVCEPEALFSLAFPDNQRPAL

--------------------------------------------------------------

>22079_22079_11_DDX5-ETV4_DDX5_chr17_62500339_ENST00000578804_ETV4_chr17_41613847_ENST00000545954_length(amino acids)=548AA_BP=131
MLFVWGLGSCRGGCHLVAVEGVERERIVRMKAGRFEADEGTSVRFGAPRFGGSRAGPLSGKKFGNPGEKLVKKKWNLDELPKFEKNFYQE
HPDLARRTAQEVETYRRSKEITVRGHNCPKPVLNFYEANFPAQVPDSDEQFVPDFHSENLAFHSPTTRIKKEPQSPRTDPALSCSRKPPL
PYHHGEQCLYSSAYDPPRQIAIKSPAPGALGQSPLQPFPRAEQRNFLRSSGTSQPHPGHGYLGEHSSVFQQPLDICHSFTSQGGGREPLP
APYQHQLSEPCPPYPQQSFKQEYHDPLYEQAGQPAVDQGGVNGHRYPGAGVVIKQEQTDFAYDSDVTGCASMYLHTEGFSGPSPGDGAMG
YGYEKPLRPFPDDVCVVPEKFEGDIKQEGVGAFREGPPYQRRGALQLWQFLVALLDDPTNAHFIAWTGRGMEFKLIEPEEVARLWGIQKN
RPAMNYDKLSRSLRYYYEKGIMQKVAGERYVYKFVCEPEALFSLAFPDNQRPALKAEFDRPVSEEDTVPLSHLDESPAYLPELAGPAQPF

--------------------------------------------------------------

>22079_22079_12_DDX5-ETV4_DDX5_chr17_62500339_ENST00000578804_ETV4_chr17_41613847_ENST00000591713_length(amino acids)=548AA_BP=131
MLFVWGLGSCRGGCHLVAVEGVERERIVRMKAGRFEADEGTSVRFGAPRFGGSRAGPLSGKKFGNPGEKLVKKKWNLDELPKFEKNFYQE
HPDLARRTAQEVETYRRSKEITVRGHNCPKPVLNFYEANFPAQVPDSDEQFVPDFHSENLAFHSPTTRIKKEPQSPRTDPALSCSRKPPL
PYHHGEQCLYSSAYDPPRQIAIKSPAPGALGQSPLQPFPRAEQRNFLRSSGTSQPHPGHGYLGEHSSVFQQPLDICHSFTSQGGGREPLP
APYQHQLSEPCPPYPQQSFKQEYHDPLYEQAGQPAVDQGGVNGHRYPGAGVVIKQEQTDFAYDSDVTGCASMYLHTEGFSGPSPGDGAMG
YGYEKPLRPFPDDVCVVPEKFEGDIKQEGVGAFREGPPYQRRGALQLWQFLVALLDDPTNAHFIAWTGRGMEFKLIEPEEVARLWGIQKN
RPAMNYDKLSRSLRYYYEKGIMQKVAGERYVYKFVCEPEALFSLAFPDNQRPALKAEFDRPVSEEDTVPLSHLDESPAYLPELAGPAQPF

--------------------------------------------------------------

>22079_22079_13_DDX5-ETV4_DDX5_chr17_62500340_ENST00000225792_ETV4_chr17_41613848_ENST00000319349_length(amino acids)=582AA_BP=165
MPESGRHLIHFYRSLVVQLRLVSSVSFLRCRPRKASPSSRPFPATCRTLFYILRSPPTATIDAMSGYSSDRDRGRDRGFGAPRFGGSRAG
PLSGKKFGNPGEKLVKKKWNLDELPKFEKNFYQEHPDLARRTAQEVETYRRSKEITVRGHNCPKPVLNFYEANFPAQVPDSDEQFVPDFH
SENLAFHSPTTRIKKEPQSPRTDPALSCSRKPPLPYHHGEQCLYSSAYDPPRQIAIKSPAPGALGQSPLQPFPRAEQRNFLRSSGTSQPH
PGHGYLGEHSSVFQQPLDICHSFTSQGGGREPLPAPYQHQLSEPCPPYPQQSFKQEYHDPLYEQAGQPAVDQGGVNGHRYPGAGVVIKQE
QTDFAYDSDVTGCASMYLHTEGFSGPSPGDGAMGYGYEKPLRPFPDDVCVVPEKFEGDIKQEGVGAFREGPPYQRRGALQLWQFLVALLD
DPTNAHFIAWTGRGMEFKLIEPEEVARLWGIQKNRPAMNYDKLSRSLRYYYEKGIMQKVAGERYVYKFVCEPEALFSLAFPDNQRPALKA

--------------------------------------------------------------

>22079_22079_14_DDX5-ETV4_DDX5_chr17_62500340_ENST00000225792_ETV4_chr17_41613848_ENST00000393664_length(amino acids)=582AA_BP=165
MPESGRHLIHFYRSLVVQLRLVSSVSFLRCRPRKASPSSRPFPATCRTLFYILRSPPTATIDAMSGYSSDRDRGRDRGFGAPRFGGSRAG
PLSGKKFGNPGEKLVKKKWNLDELPKFEKNFYQEHPDLARRTAQEVETYRRSKEITVRGHNCPKPVLNFYEANFPAQVPDSDEQFVPDFH
SENLAFHSPTTRIKKEPQSPRTDPALSCSRKPPLPYHHGEQCLYSSAYDPPRQIAIKSPAPGALGQSPLQPFPRAEQRNFLRSSGTSQPH
PGHGYLGEHSSVFQQPLDICHSFTSQGGGREPLPAPYQHQLSEPCPPYPQQSFKQEYHDPLYEQAGQPAVDQGGVNGHRYPGAGVVIKQE
QTDFAYDSDVTGCASMYLHTEGFSGPSPGDGAMGYGYEKPLRPFPDDVCVVPEKFEGDIKQEGVGAFREGPPYQRRGALQLWQFLVALLD
DPTNAHFIAWTGRGMEFKLIEPEEVARLWGIQKNRPAMNYDKLSRSLRYYYEKGIMQKVAGERYVYKFVCEPEALFSLAFPDNQRPALKA

--------------------------------------------------------------

>22079_22079_15_DDX5-ETV4_DDX5_chr17_62500340_ENST00000225792_ETV4_chr17_41613848_ENST00000538265_length(amino acids)=582AA_BP=165
MPESGRHLIHFYRSLVVQLRLVSSVSFLRCRPRKASPSSRPFPATCRTLFYILRSPPTATIDAMSGYSSDRDRGRDRGFGAPRFGGSRAG
PLSGKKFGNPGEKLVKKKWNLDELPKFEKNFYQEHPDLARRTAQEVETYRRSKEITVRGHNCPKPVLNFYEANFPAQVPDSDEQFVPDFH
SENLAFHSPTTRIKKEPQSPRTDPALSCSRKPPLPYHHGEQCLYSSAYDPPRQIAIKSPAPGALGQSPLQPFPRAEQRNFLRSSGTSQPH
PGHGYLGEHSSVFQQPLDICHSFTSQGGGREPLPAPYQHQLSEPCPPYPQQSFKQEYHDPLYEQAGQPAVDQGGVNGHRYPGAGVVIKQE
QTDFAYDSDVTGCASMYLHTEGFSGPSPGDGAMGYGYEKPLRPFPDDVCVVPEKFEGDIKQEGVGAFREGPPYQRRGALQLWQFLVALLD
DPTNAHFIAWTGRGMEFKLIEPEEVARLWGIQKNRPAMNYDKLSRSLRYYYEKGIMQKVAGERYVYKFVCEPEALFSLAFPDNQRPALKA

--------------------------------------------------------------

>22079_22079_16_DDX5-ETV4_DDX5_chr17_62500340_ENST00000225792_ETV4_chr17_41613848_ENST00000545089_length(amino acids)=528AA_BP=165
MPESGRHLIHFYRSLVVQLRLVSSVSFLRCRPRKASPSSRPFPATCRTLFYILRSPPTATIDAMSGYSSDRDRGRDRGFGAPRFGGSRAG
PLSGKKFGNPGEKLVKKKWNLDELPKFEKNFYQEHPDLARRTAQEVETYRRSKEITVRGHNCPKPVLNFYEANFPAQVPDSDEQFVPDFH
SENLAFHSPTTRIKKEPQSPRTDPALSCSRKPPLPYHHGEQCLYSSSVFQQPLDICHSFTSQGGGREPLPAPYQHQLSEPCPPYPQQSFK
QEYHDPLYEQAGQPAVDQGGVNGHRYPGAGVVIKQEQTDFAYDSDVTGCASMYLHTEGFSGPSPGDGAMGYGYEKPLRPFPDDVCVVPEK
FEGDIKQEGVGAFREGPPYQRRGALQLWQFLVALLDDPTNAHFIAWTGRGMEFKLIEPEEVARLWGIQKNRPAMNYDKLSRSLRYYYEKG

--------------------------------------------------------------

>22079_22079_17_DDX5-ETV4_DDX5_chr17_62500340_ENST00000225792_ETV4_chr17_41613848_ENST00000545954_length(amino acids)=582AA_BP=165
MPESGRHLIHFYRSLVVQLRLVSSVSFLRCRPRKASPSSRPFPATCRTLFYILRSPPTATIDAMSGYSSDRDRGRDRGFGAPRFGGSRAG
PLSGKKFGNPGEKLVKKKWNLDELPKFEKNFYQEHPDLARRTAQEVETYRRSKEITVRGHNCPKPVLNFYEANFPAQVPDSDEQFVPDFH
SENLAFHSPTTRIKKEPQSPRTDPALSCSRKPPLPYHHGEQCLYSSAYDPPRQIAIKSPAPGALGQSPLQPFPRAEQRNFLRSSGTSQPH
PGHGYLGEHSSVFQQPLDICHSFTSQGGGREPLPAPYQHQLSEPCPPYPQQSFKQEYHDPLYEQAGQPAVDQGGVNGHRYPGAGVVIKQE
QTDFAYDSDVTGCASMYLHTEGFSGPSPGDGAMGYGYEKPLRPFPDDVCVVPEKFEGDIKQEGVGAFREGPPYQRRGALQLWQFLVALLD
DPTNAHFIAWTGRGMEFKLIEPEEVARLWGIQKNRPAMNYDKLSRSLRYYYEKGIMQKVAGERYVYKFVCEPEALFSLAFPDNQRPALKA

--------------------------------------------------------------

>22079_22079_18_DDX5-ETV4_DDX5_chr17_62500340_ENST00000225792_ETV4_chr17_41613848_ENST00000591713_length(amino acids)=582AA_BP=165
MPESGRHLIHFYRSLVVQLRLVSSVSFLRCRPRKASPSSRPFPATCRTLFYILRSPPTATIDAMSGYSSDRDRGRDRGFGAPRFGGSRAG
PLSGKKFGNPGEKLVKKKWNLDELPKFEKNFYQEHPDLARRTAQEVETYRRSKEITVRGHNCPKPVLNFYEANFPAQVPDSDEQFVPDFH
SENLAFHSPTTRIKKEPQSPRTDPALSCSRKPPLPYHHGEQCLYSSAYDPPRQIAIKSPAPGALGQSPLQPFPRAEQRNFLRSSGTSQPH
PGHGYLGEHSSVFQQPLDICHSFTSQGGGREPLPAPYQHQLSEPCPPYPQQSFKQEYHDPLYEQAGQPAVDQGGVNGHRYPGAGVVIKQE
QTDFAYDSDVTGCASMYLHTEGFSGPSPGDGAMGYGYEKPLRPFPDDVCVVPEKFEGDIKQEGVGAFREGPPYQRRGALQLWQFLVALLD
DPTNAHFIAWTGRGMEFKLIEPEEVARLWGIQKNRPAMNYDKLSRSLRYYYEKGIMQKVAGERYVYKFVCEPEALFSLAFPDNQRPALKA

--------------------------------------------------------------

>22079_22079_19_DDX5-ETV4_DDX5_chr17_62500340_ENST00000578804_ETV4_chr17_41613848_ENST00000319349_length(amino acids)=548AA_BP=131
MLFVWGLGSCRGGCHLVAVEGVERERIVRMKAGRFEADEGTSVRFGAPRFGGSRAGPLSGKKFGNPGEKLVKKKWNLDELPKFEKNFYQE
HPDLARRTAQEVETYRRSKEITVRGHNCPKPVLNFYEANFPAQVPDSDEQFVPDFHSENLAFHSPTTRIKKEPQSPRTDPALSCSRKPPL
PYHHGEQCLYSSAYDPPRQIAIKSPAPGALGQSPLQPFPRAEQRNFLRSSGTSQPHPGHGYLGEHSSVFQQPLDICHSFTSQGGGREPLP
APYQHQLSEPCPPYPQQSFKQEYHDPLYEQAGQPAVDQGGVNGHRYPGAGVVIKQEQTDFAYDSDVTGCASMYLHTEGFSGPSPGDGAMG
YGYEKPLRPFPDDVCVVPEKFEGDIKQEGVGAFREGPPYQRRGALQLWQFLVALLDDPTNAHFIAWTGRGMEFKLIEPEEVARLWGIQKN
RPAMNYDKLSRSLRYYYEKGIMQKVAGERYVYKFVCEPEALFSLAFPDNQRPALKAEFDRPVSEEDTVPLSHLDESPAYLPELAGPAQPF

--------------------------------------------------------------

>22079_22079_20_DDX5-ETV4_DDX5_chr17_62500340_ENST00000578804_ETV4_chr17_41613848_ENST00000393664_length(amino acids)=548AA_BP=131
MLFVWGLGSCRGGCHLVAVEGVERERIVRMKAGRFEADEGTSVRFGAPRFGGSRAGPLSGKKFGNPGEKLVKKKWNLDELPKFEKNFYQE
HPDLARRTAQEVETYRRSKEITVRGHNCPKPVLNFYEANFPAQVPDSDEQFVPDFHSENLAFHSPTTRIKKEPQSPRTDPALSCSRKPPL
PYHHGEQCLYSSAYDPPRQIAIKSPAPGALGQSPLQPFPRAEQRNFLRSSGTSQPHPGHGYLGEHSSVFQQPLDICHSFTSQGGGREPLP
APYQHQLSEPCPPYPQQSFKQEYHDPLYEQAGQPAVDQGGVNGHRYPGAGVVIKQEQTDFAYDSDVTGCASMYLHTEGFSGPSPGDGAMG
YGYEKPLRPFPDDVCVVPEKFEGDIKQEGVGAFREGPPYQRRGALQLWQFLVALLDDPTNAHFIAWTGRGMEFKLIEPEEVARLWGIQKN
RPAMNYDKLSRSLRYYYEKGIMQKVAGERYVYKFVCEPEALFSLAFPDNQRPALKAEFDRPVSEEDTVPLSHLDESPAYLPELAGPAQPF

--------------------------------------------------------------

>22079_22079_21_DDX5-ETV4_DDX5_chr17_62500340_ENST00000578804_ETV4_chr17_41613848_ENST00000538265_length(amino acids)=548AA_BP=131
MLFVWGLGSCRGGCHLVAVEGVERERIVRMKAGRFEADEGTSVRFGAPRFGGSRAGPLSGKKFGNPGEKLVKKKWNLDELPKFEKNFYQE
HPDLARRTAQEVETYRRSKEITVRGHNCPKPVLNFYEANFPAQVPDSDEQFVPDFHSENLAFHSPTTRIKKEPQSPRTDPALSCSRKPPL
PYHHGEQCLYSSAYDPPRQIAIKSPAPGALGQSPLQPFPRAEQRNFLRSSGTSQPHPGHGYLGEHSSVFQQPLDICHSFTSQGGGREPLP
APYQHQLSEPCPPYPQQSFKQEYHDPLYEQAGQPAVDQGGVNGHRYPGAGVVIKQEQTDFAYDSDVTGCASMYLHTEGFSGPSPGDGAMG
YGYEKPLRPFPDDVCVVPEKFEGDIKQEGVGAFREGPPYQRRGALQLWQFLVALLDDPTNAHFIAWTGRGMEFKLIEPEEVARLWGIQKN
RPAMNYDKLSRSLRYYYEKGIMQKVAGERYVYKFVCEPEALFSLAFPDNQRPALKAEFDRPVSEEDTVPLSHLDESPAYLPELAGPAQPF

--------------------------------------------------------------

>22079_22079_22_DDX5-ETV4_DDX5_chr17_62500340_ENST00000578804_ETV4_chr17_41613848_ENST00000545089_length(amino acids)=494AA_BP=131
MLFVWGLGSCRGGCHLVAVEGVERERIVRMKAGRFEADEGTSVRFGAPRFGGSRAGPLSGKKFGNPGEKLVKKKWNLDELPKFEKNFYQE
HPDLARRTAQEVETYRRSKEITVRGHNCPKPVLNFYEANFPAQVPDSDEQFVPDFHSENLAFHSPTTRIKKEPQSPRTDPALSCSRKPPL
PYHHGEQCLYSSSVFQQPLDICHSFTSQGGGREPLPAPYQHQLSEPCPPYPQQSFKQEYHDPLYEQAGQPAVDQGGVNGHRYPGAGVVIK
QEQTDFAYDSDVTGCASMYLHTEGFSGPSPGDGAMGYGYEKPLRPFPDDVCVVPEKFEGDIKQEGVGAFREGPPYQRRGALQLWQFLVAL
LDDPTNAHFIAWTGRGMEFKLIEPEEVARLWGIQKNRPAMNYDKLSRSLRYYYEKGIMQKVAGERYVYKFVCEPEALFSLAFPDNQRPAL

--------------------------------------------------------------

>22079_22079_23_DDX5-ETV4_DDX5_chr17_62500340_ENST00000578804_ETV4_chr17_41613848_ENST00000545954_length(amino acids)=548AA_BP=131
MLFVWGLGSCRGGCHLVAVEGVERERIVRMKAGRFEADEGTSVRFGAPRFGGSRAGPLSGKKFGNPGEKLVKKKWNLDELPKFEKNFYQE
HPDLARRTAQEVETYRRSKEITVRGHNCPKPVLNFYEANFPAQVPDSDEQFVPDFHSENLAFHSPTTRIKKEPQSPRTDPALSCSRKPPL
PYHHGEQCLYSSAYDPPRQIAIKSPAPGALGQSPLQPFPRAEQRNFLRSSGTSQPHPGHGYLGEHSSVFQQPLDICHSFTSQGGGREPLP
APYQHQLSEPCPPYPQQSFKQEYHDPLYEQAGQPAVDQGGVNGHRYPGAGVVIKQEQTDFAYDSDVTGCASMYLHTEGFSGPSPGDGAMG
YGYEKPLRPFPDDVCVVPEKFEGDIKQEGVGAFREGPPYQRRGALQLWQFLVALLDDPTNAHFIAWTGRGMEFKLIEPEEVARLWGIQKN
RPAMNYDKLSRSLRYYYEKGIMQKVAGERYVYKFVCEPEALFSLAFPDNQRPALKAEFDRPVSEEDTVPLSHLDESPAYLPELAGPAQPF

--------------------------------------------------------------

>22079_22079_24_DDX5-ETV4_DDX5_chr17_62500340_ENST00000578804_ETV4_chr17_41613848_ENST00000591713_length(amino acids)=548AA_BP=131
MLFVWGLGSCRGGCHLVAVEGVERERIVRMKAGRFEADEGTSVRFGAPRFGGSRAGPLSGKKFGNPGEKLVKKKWNLDELPKFEKNFYQE
HPDLARRTAQEVETYRRSKEITVRGHNCPKPVLNFYEANFPAQVPDSDEQFVPDFHSENLAFHSPTTRIKKEPQSPRTDPALSCSRKPPL
PYHHGEQCLYSSAYDPPRQIAIKSPAPGALGQSPLQPFPRAEQRNFLRSSGTSQPHPGHGYLGEHSSVFQQPLDICHSFTSQGGGREPLP
APYQHQLSEPCPPYPQQSFKQEYHDPLYEQAGQPAVDQGGVNGHRYPGAGVVIKQEQTDFAYDSDVTGCASMYLHTEGFSGPSPGDGAMG
YGYEKPLRPFPDDVCVVPEKFEGDIKQEGVGAFREGPPYQRRGALQLWQFLVALLDDPTNAHFIAWTGRGMEFKLIEPEEVARLWGIQKN
RPAMNYDKLSRSLRYYYEKGIMQKVAGERYVYKFVCEPEALFSLAFPDNQRPALKAEFDRPVSEEDTVPLSHLDESPAYLPELAGPAQPF

--------------------------------------------------------------

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Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr17:62502407/chr17:41605887)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
DDX5 Q5T1V6	ETV4 P43268
	FUNCTION: Transcriptional activator (PubMed:19307308, PubMed:31552090). May play a role in keratinocyte differentiation (PubMed:31552090). {ECO:0000269\|PubMed:19307308, ECO:0000269\|PubMed:31552090}.; FUNCTION: (Microbial infection) Binds to the enhancer of the adenovirus E1A gene and acts as a transcriptional activator; the core-binding sequence is 5'-[AC]GGA[AT]GT-3'. {ECO:0000269\|PubMed:8441666}.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Tgene

ETV4

chr17:62500340

chr17:41613848

ENST00000319349

148_244

67.33333333333333

485.0

Compositional bias

Note=Gln-rich

Tgene

ETV4

chr17:62500340

chr17:41613848

ENST00000393664

148_244

67.33333333333333

485.0

Compositional bias

Note=Gln-rich

Tgene

ETV4

chr17:62500340

chr17:41613848

ENST00000538265

148_244

28.333333333333332

446.0

Compositional bias

Note=Gln-rich

Tgene

ETV4

chr17:62500340

chr17:41613848

ENST00000538265

49_75

28.333333333333332

446.0

Compositional bias

Note=Asp/Glu-rich (acidic)

Tgene

ETV4

chr17:62500340

chr17:41613848

ENST00000545954

148_244

28.333333333333332

446.0

Compositional bias

Note=Gln-rich

Tgene

ETV4

chr17:62500340

chr17:41613848

ENST00000545954

49_75

28.333333333333332

446.0

Compositional bias

Note=Asp/Glu-rich (acidic)

Tgene

ETV4

chr17:62500340

chr17:41613848

ENST00000591713

148_244

67.33333333333333

485.0

Compositional bias

Note=Gln-rich

Tgene

ETV4

chr17:62500340

chr17:41613848

ENST00000319349

341_421

67.33333333333333

485.0

DNA binding

ETS

Tgene

ETV4

chr17:62500340

chr17:41613848

ENST00000393664

341_421

67.33333333333333

485.0

DNA binding

ETS

Tgene

ETV4

chr17:62500340

chr17:41613848

ENST00000538265

341_421

28.333333333333332

446.0

DNA binding

ETS

Tgene

ETV4

chr17:62500340

chr17:41613848

ENST00000545954

341_421

28.333333333333332

446.0

DNA binding

ETS

Tgene

ETV4

chr17:62500340

chr17:41613848

ENST00000591713

341_421

67.33333333333333

485.0

DNA binding

ETS

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

DDX5

chr17:62500340

chr17:41613848

ENST00000225792

125_300

102.33333333333333

615.0

Domain

Helicase ATP-binding

Hgene

DDX5

chr17:62500340

chr17:41613848

ENST00000225792

328_475

102.33333333333333

615.0

Domain

Helicase C-terminal

Hgene

DDX5

chr17:62500340

chr17:41613848

ENST00000225792

248_251

102.33333333333333

615.0

Motif

Note=DEAD box

Hgene

DDX5

chr17:62500340

chr17:41613848

ENST00000225792

94_122

102.33333333333333

615.0

Motif

Note=Q motif

Hgene

DDX5

chr17:62500340

chr17:41613848

ENST00000225792

114_116

102.33333333333333

615.0

Nucleotide binding

Note=ATP

Hgene

DDX5

chr17:62500340

chr17:41613848

ENST00000225792

138_145

102.33333333333333

615.0

Nucleotide binding

Note=ATP

Hgene

DDX5

chr17:62500340

chr17:41613848

ENST00000225792

477_614

102.33333333333333

615.0

Region

Note=Transactivation domain

Tgene

ETV4

chr17:62500340

chr17:41613848

ENST00000319349

49_75

67.33333333333333

485.0

Compositional bias

Note=Asp/Glu-rich (acidic)

Tgene

ETV4

chr17:62500340

chr17:41613848

ENST00000393664

49_75

67.33333333333333

485.0

Compositional bias

Note=Asp/Glu-rich (acidic)

Tgene

ETV4

chr17:62500340

chr17:41613848

ENST00000591713

49_75

67.33333333333333

485.0

Compositional bias

Note=Asp/Glu-rich (acidic)

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Fusion Protein Structures

PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.

Fusion protein PDB link (fusion AA seq ID in FusionPDB)	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	AA seq	Len(AA seq)

PDB file (289) >>>289.pdbFusion protein BP residue: 131 CIF file (289) >>>289.cif	DDX5	chr17	62500339	-	ETV4	chr17	41613847	-	MLFVWGLGSCRGGCHLVAVEGVERERIVRMKAGRFEADEGTSVRFGAPRF GGSRAGPLSGKKFGNPGEKLVKKKWNLDELPKFEKNFYQEHPDLARRTAQ EVETYRRSKEITVRGHNCPKPVLNFYEANFPAQVPDSDEQFVPDFHSENL AFHSPTTRIKKEPQSPRTDPALSCSRKPPLPYHHGEQCLYSSSVFQQPLD ICHSFTSQGGGREPLPAPYQHQLSEPCPPYPQQSFKQEYHDPLYEQAGQP AVDQGGVNGHRYPGAGVVIKQEQTDFAYDSDVTGCASMYLHTEGFSGPSP GDGAMGYGYEKPLRPFPDDVCVVPEKFEGDIKQEGVGAFREGPPYQRRGA LQLWQFLVALLDDPTNAHFIAWTGRGMEFKLIEPEEVARLWGIQKNRPAM NYDKLSRSLRYYYEKGIMQKVAGERYVYKFVCEPEALFSLAFPDNQRPAL	494
3D view using mol* of 289 (AA BP:131)


PDB file (356) >>>356.pdbFusion protein BP residue: 165 CIF file (356) >>>356.cif	DDX5	chr17	62500339	-	ETV4	chr17	41613847	-	MPESGRHLIHFYRSLVVQLRLVSSVSFLRCRPRKASPSSRPFPATCRTLF YILRSPPTATIDAMSGYSSDRDRGRDRGFGAPRFGGSRAGPLSGKKFGNP GEKLVKKKWNLDELPKFEKNFYQEHPDLARRTAQEVETYRRSKEITVRGH NCPKPVLNFYEANFPAQVPDSDEQFVPDFHSENLAFHSPTTRIKKEPQSP RTDPALSCSRKPPLPYHHGEQCLYSSSVFQQPLDICHSFTSQGGGREPLP APYQHQLSEPCPPYPQQSFKQEYHDPLYEQAGQPAVDQGGVNGHRYPGAG VVIKQEQTDFAYDSDVTGCASMYLHTEGFSGPSPGDGAMGYGYEKPLRPF PDDVCVVPEKFEGDIKQEGVGAFREGPPYQRRGALQLWQFLVALLDDPTN AHFIAWTGRGMEFKLIEPEEVARLWGIQKNRPAMNYDKLSRSLRYYYEKG IMQKVAGERYVYKFVCEPEALFSLAFPDNQRPALKAEFDRPVSEEDTVPL	528
3D view using mol* of 356 (AA BP:165)


PDB file (388) >>>388.pdbFusion protein BP residue: 131 CIF file (388) >>>388.cif	DDX5	chr17	62500339	-	ETV4	chr17	41613847	-	MLFVWGLGSCRGGCHLVAVEGVERERIVRMKAGRFEADEGTSVRFGAPRF GGSRAGPLSGKKFGNPGEKLVKKKWNLDELPKFEKNFYQEHPDLARRTAQ EVETYRRSKEITVRGHNCPKPVLNFYEANFPAQVPDSDEQFVPDFHSENL AFHSPTTRIKKEPQSPRTDPALSCSRKPPLPYHHGEQCLYSSAYDPPRQI AIKSPAPGALGQSPLQPFPRAEQRNFLRSSGTSQPHPGHGYLGEHSSVFQ QPLDICHSFTSQGGGREPLPAPYQHQLSEPCPPYPQQSFKQEYHDPLYEQ AGQPAVDQGGVNGHRYPGAGVVIKQEQTDFAYDSDVTGCASMYLHTEGFS GPSPGDGAMGYGYEKPLRPFPDDVCVVPEKFEGDIKQEGVGAFREGPPYQ RRGALQLWQFLVALLDDPTNAHFIAWTGRGMEFKLIEPEEVARLWGIQKN RPAMNYDKLSRSLRYYYEKGIMQKVAGERYVYKFVCEPEALFSLAFPDNQ	548
3D view using mol* of 388 (AA BP:131)


PDB file (446) >>>446.pdbFusion protein BP residue: 165 CIF file (446) >>>446.cif	DDX5	chr17	62500339	-	ETV4	chr17	41613847	-	MPESGRHLIHFYRSLVVQLRLVSSVSFLRCRPRKASPSSRPFPATCRTLF YILRSPPTATIDAMSGYSSDRDRGRDRGFGAPRFGGSRAGPLSGKKFGNP GEKLVKKKWNLDELPKFEKNFYQEHPDLARRTAQEVETYRRSKEITVRGH NCPKPVLNFYEANFPAQVPDSDEQFVPDFHSENLAFHSPTTRIKKEPQSP RTDPALSCSRKPPLPYHHGEQCLYSSAYDPPRQIAIKSPAPGALGQSPLQ PFPRAEQRNFLRSSGTSQPHPGHGYLGEHSSVFQQPLDICHSFTSQGGGR EPLPAPYQHQLSEPCPPYPQQSFKQEYHDPLYEQAGQPAVDQGGVNGHRY PGAGVVIKQEQTDFAYDSDVTGCASMYLHTEGFSGPSPGDGAMGYGYEKP LRPFPDDVCVVPEKFEGDIKQEGVGAFREGPPYQRRGALQLWQFLVALLD DPTNAHFIAWTGRGMEFKLIEPEEVARLWGIQKNRPAMNYDKLSRSLRYY YEKGIMQKVAGERYVYKFVCEPEALFSLAFPDNQRPALKAEFDRPVSEED	582
3D view using mol* of 446 (AA BP:165)

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pLDDT score distribution

pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

ETV4_pLDDT.png

pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

DDX5_ETV4_289_pLDDT.png (AA BP:131)

DDX5_ETV4_289_pLDDT_and_active_sites.png (AA BP:131)

DDX5_ETV4_289_violinplot.png (AA BP:131)

DDX5_ETV4_356_pLDDT.png (AA BP:165)

DDX5_ETV4_356_pLDDT_and_active_sites.png (AA BP:165)

DDX5_ETV4_356_violinplot.png (AA BP:165)

DDX5_ETV4_388_pLDDT.png (AA BP:131)

DDX5_ETV4_388_pLDDT_and_active_sites.png (AA BP:131)

DDX5_ETV4_388_violinplot.png (AA BP:131)

DDX5_ETV4_446_pLDDT.png (AA BP:165)

DDX5_ETV4_446_pLDDT_and_active_sites.png (AA BP:165)

DDX5_ETV4_446_violinplot.png (AA BP:165)

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Ramachandran Plot of Fusion Protein Structure

Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.

Fusion AA seq ID in FusionPDB and their Ramachandran plots

DDX5_ETV4_289.png

DDX5_ETV4_356.png

DDX5_ETV4_388.png

DDX5_ETV4_446.png

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Potential Active Site Information

The potential binding sites of these fusion proteins were identified using SiteMap, a module of the Schrodinger suite.

Fusion AA seq ID in FusionPDB	Site score	Size	D score	Volume	Exposure	Enclosure	Contact	Phobic	Philic	Balance	Don/Acc	Residues
289	1.025	260	1.072	707.266	0.552	0.679	0.891	0.856	0.846	1.012	0.655	Chain A: 2,3,4,5,6,7,8,9,10,14,15,16,18,19,20,21,8 5,86,87,88,89,90,94,105,106,109,110,111,113,114,11 8,119,120,121,122,123,125,128,129,130,131
356	1.04	516	1.106	1912.911	0.639	0.662	0.833	0.877	0.708	1.238	1.104	Chain A: 4,7,8,11,12,14,15,17,18,19,21,22,23,25,26 ,27,28,29,30,31,32,33,34,35,42,43,44,45,46,47,48,4 9,50,51,52,53,54,55,56,57,58,59,114,117,119,120,12 1,122,123,140,143,144,145,146,147,148,149,150,151, 152,153,154,155,156,157,158,159,162,163,164,165,38 6,387,388,410,425,429,431,434,438,439,442,445,446, 449,451,466,468,471,472,473,474,476,477,478,479,48 1,482,484
388	1.0666	119	1.1201	641.41	0.598	0.7209	0.9341	1.0176	0.7745	1.3138	0.8029	Chain A: 5,8,9,10,11,12,14,15,16,29,30,31,32,85,86 ,87,88,90,94,105,106,111,113,117,118,119,120,121,1 22,128
446	1.031	170	1.108	558.061	0.692	0.63	0.853	0.987	0.645	1.531	0.798	Chain A: 12,13,16,17,20,23,24,26,27,430,431,432,43 3,434,435,436,437,438,439,440,442,443,444,446,447, 448,451,454,457,478,481,482,483,484,530,531,532,53 4,536

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Potentially Interacting Small Molecules through Virtual Screening

The FDA-approved small molecule library molecules were subjected to virtual screening using the Glide.

Fusion AA seq ID in FusionPDB

ZINC ID

DrugBank ID

Drug name

Docking score

Glide gscore

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Drug information from DrugBank of the top 20 interacting small molecules.

ZINC ID

DrugBank ID

Drug name

Drug type

SMILES

Drug group

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Biochemical Features of Small Molecules

ADME (Absorption, Distribution, Metabolism, and Excretion) of drugs using QikProp(v3.9)

ZINC ID

mol_MW

dipole

SASA

FOSA

FISA

PISA

WPSA

volume

donorHB

accptHB

Human Oral Absorption

Percent Human Oral Absorption

Rule Of Five

Rule Of Three

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Drug Toxicity Information

Toxicity information of individual drugs using eToxPred

ZINC ID

Smile

Surface Accessibility

Toxicity

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Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

DDX5

STK24, HDAC1, CREBBP, EP300, AKAP8, FBL, OTUB1, MEPCE, CDK9, HNRNPA1, HNRNPH1, KHDRBS2, IPO11, TCEA1, AIRE, MYOD1, SMARCA4, TBP, POLR2A, MGMT, SRRM1, SRRM2, tat, PIAS1, MBD3, CHD3, ESR1, TADA2A, SMN1, CD4, DGCR8, ZWINT, Cdk1, AI837181, Ybx1, CALM1, EBNA-LP, PIK3CA, PSMA3, TNNT1, VHL, SMARCAD1, YY1, NDRG1, HDGF, CENPA, MBNL1, SF3A2, ARRB2, ERG, NFX1, CUL3, CUL4A, CUL4B, CUL5, CUL2, CUL1, COPS5, COPS6, DCUN1D1, CAND1, NEDD8, DDX5, DDX17, HDAC2, HDAC3, BBLF2-BBLF3, INPPL1, SHC1, CRK, AKT1, DHX15, SRSF1, SF3B3, U2AF2, DHX38, SNRPA1, EIF4A3, PRKDC, SF3A1, HNRNPM, HNRNPF, SNRPD1, RBMX, SYNCRIP, U2AF1, SART1, PRPF40A, IGF2BP3, SNW1, PRPF19, SNRPD2, VTN, MCM7, SYN1, RPS20, XRCC4, RPS16, HNRNPA0, EEF2, PRSS1, THRAP3, GOLIM4, EPRS, RPL19, SRP14, SMURF1, FN1, VCAM1, RBM10, WBP11, HNRNPK, DHX9, RBM4, HNRNPH2, HNRNPH3, KHDRBS1, RBFOX2, IL7R, MAP1LC3A, ITGA4, CTNNB1, CD81, IGSF8, FBXW4, FBXO46, SP1, MAPK10, FBXO6, TARDBP, PARK2, PIN1, DBNL, ERO1L, FKBP10, LRSAM1, PABPC4, PRPSAP1, WDR4, rev, RPA1, RPA2, RPA3, STAU1, MDM2, SPRTN, AURKA, AURKB, CEP250, TP53, TUBGCP3, FUS, MAPKAPK2, DROSHA, NXF1, CUL7, OBSL1, CCDC8, UBE2I, EZH2, SUZ12, EED, RNF2, SMAD3, ABL1, ARAF, CDK2, DAPK1, ILK, MAST3, SGK2, SRPK1, TNK1, TYK2, LUZP4, RPS6KB2, UNK, NTRK1, ATXN2, ATXN2L, C14orf166, CPSF6, DDX18, DNAJC2, ELAC2, GTPBP4, HNRNPR, IMPDH1, SBDS, SKIV2L2, TRMT6, DHX36, EDC4, EIF2A, GMPS, LUC7L2, NACA, UPF1, SFN, SCARNA22, FRS3, gag, XPO1, HNRNPU, RPL10, MATR3, Bcas2, Bag2, Fus, SKI, MCM2, MCM5, Ksr1, CDC5L, CDC37, CRBN, CBX7, RYBP, WWP2, DERL1, EGFR, YPEL5, RMND5A, MAEA, RANBP9, ARMC8, NR2E1, CYLD, CD2BP2, INO80B, TRIM25, HOTAIR, HEY1, BRCA1, LMNA, MTF1, FBXO7, IQGAP1, TMPO, WDR77, MTCH2, PCBP1, PPIE, CCNT1, GSK3A, MAP2K3, MED12, POLD1, TGFB1, TRIP4, ZNF217, PRPF8, EFTUD2, AAR2, PIH1D1, TNIP2, CHD4, LARP7, RNF4, TNF, RIOK1, SQSTM1, HEXIM1, RUNX1, DPF2, RNF123, NBN, SNAI1, AGR2, RECQL4, ZFP36L2, CNTNAP1, MYC, Prkaa1, Prkab1, CDK5RAP2, METTL3, METTL14, WTAP, KIAA1429, PSME3, PHF1, PHB, FAF1, RBX1, KIN, USP14, NR2C2, UCHL3, PPP1CC, SETD1A, ZFYVE21, XRCC6, CTCF, AGRN, VRK1, HIST1H4A, PSMD14, APEX1, SNRNP70, EWSR1, TAF15, SNRPC, ITFG1, GSK3B, CDC6, BIRC3, BRD7, ERCC6, SOX2, TRIM28, CMTR1, PLEKHA4, MAGEA3, KCTD10, PINK1, YAP1, TFCP2, FANCD2, ORF50, ZC3H18, PTK6, STAG2, MAU2, HSCB, CELF1, ELAVL1, DUX4L9, DUX4, CIT, ANLN, CHMP4B, CHMP4C, ECT2, KIF14, KIF20A, KIF23, PRC1, ACACA, C12orf49, INS, SUMO2, NDN, BRD4, NUPR1, RBM45, CIC, Apc2, RBM39, FBP1, nsp13ab, LGALS9, IFI16, EIF3F, DDX58, OGT, SPOP, UFL1, DDRGK1, NR4A1, DDX23, NIFK, RPL31, SLC26A4-AS1, E2F1, FZR1, WDR5, PAGE4, NAA40, BTF3, AGO2, AGO1, RCHY1, NBR1, nsp14, RB1CC1, DDX3X, ZEB1,

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
DDX5
ETV4

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

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Related Drugs to DDX5-ETV4

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

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Related Diseases to DDX5-ETV4

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner	Gene	Disease ID	Disease name	# pubmeds	Source
Hgene	DDX5	C0014175	Endometriosis	1	CTD_human
Hgene	DDX5	C0269102	Endometrioma	1	CTD_human
Tgene	ETV4	C0033578	Prostatic Neoplasms	2	CTD_human
Tgene	ETV4	C0376358	Malignant neoplasm of prostate	2	CTD_human
Tgene	ETV4	C0006142	Malignant neoplasm of breast	1	CTD_human
Tgene	ETV4	C0027627	Neoplasm Metastasis	1	CTD_human
Tgene	ETV4	C0678222	Breast Carcinoma	1	CTD_human
Tgene	ETV4	C1257931	Mammary Neoplasms, Human	1	CTD_human
Tgene	ETV4	C1458155	Mammary Neoplasms	1	CTD_human
Tgene	ETV4	C4704874	Mammary Carcinoma, Human	1	CTD_human