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Fusion Protein:ADCY3-HADHA |
Fusion Protein Summary |
 Fusion gene summary |
| Fusion partner gene information | Fusion gene name: ADCY3-HADHA | FusionPDB ID: 2228 | FusionGDB2.0 ID: 2228 | Hgene | Tgene | Gene symbol | ADCY3 | HADHA | Gene ID | 114 | 3030 |
| Gene name | adenylate cyclase 8 | hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha | |
| Synonyms | AC8|ADCY3|HBAC1 | ECHA|GBP|HADH|LCEH|LCHAD|MTPA|TP-ALPHA | |
| Cytomap | 8q24.22 | 2p23.3 | |
| Type of gene | protein-coding | protein-coding | |
| Description | adenylate cyclase type 8ATP pyrophosphate-lyase 8HEL-S-172mPadenylate cyclase 8 (brain)adenylate cyclase type VIIIadenylyl cyclase 8adenylyl cyclase-8, brainca(2+)/calmodulin-activated adenylyl cyclaseepididymis secretory sperm binding protein Li | trifunctional enzyme subunit alpha, mitochondrial3-ketoacyl-Coenzyme A (CoA) thiolase, alpha subunit3-oxoacyl-CoA thiolase78 kDa gastrin-binding proteingastrin-binding proteinhydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase ( | |
| Modification date | 20200313 | 20200313 | |
| UniProtAcc | O60266 | P40939 | |
| Ensembl transtripts involved in fusion gene | ENST ids | ENST00000260600, ENST00000405392, ENST00000450524, | ENST00000457468, ENST00000461025, ENST00000380649, |
| Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0) | * DoF score | 7 X 6 X 5=210 | 7 X 6 X 7=294 |
| # samples | 7 | 9 | |
| ** MAII score | log2(7/210*10)=-1.58496250072116 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | log2(9/294*10)=-1.70781924850669 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | |
| Context (manual curation of fusion genes in FusionPDB) | PubMed: ADCY3 [Title/Abstract] AND HADHA [Title/Abstract] AND fusion [Title/Abstract] | ||
| Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0) | ADCY3(25141182)-HADHA(26417507), # samples:1 | ||
| Anticipated loss of major functional domain due to fusion event. | |||
| * DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
| Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
| Partner | Gene | GO ID | GO term | PubMed ID |
| Tgene | HADHA | GO:0035965 | cardiolipin acyl-chain remodeling | 23152787 |
| Fusion gene breakpoints across ADCY3 (5'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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| Fusion gene breakpoints across HADHA (3'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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Fusion Gene Sample Information |
| Fusion gene information from FusionGDB2.0. |
| Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0) * All genome coordinats were lifted-over on hg19. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| Source | Disease | Sample | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| ChimerDB4 | LUSC | TCGA-43-3394-01A | ADCY3 | chr2 | 25141182 | - | HADHA | chr2 | 26417507 | - |
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Fusion ORF Analysis |
| Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
| ENST00000260600 | ADCY3 | chr2 | 25141182 | - | ENST00000380649 | HADHA | chr2 | 26417507 | - | 2814 | 1527 | 852 | 2198 | 448 |
| DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
| ENST00000260600 | ENST00000380649 | ADCY3 | chr2 | 25141182 | - | HADHA | chr2 | 26417507 | - | 0.002141016 | 0.99785894 |
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Fusion Amino Acid Sequences |
| For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones. |
| >FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP >2228_2228_1_ADCY3-HADHA_ADCY3_chr2_25141182_ENST00000260600_HADHA_chr2_26417507_ENST00000380649_length(amino acids)=448AA_BP=225 MPRNQGFSEPEYSAEYSAEYSVSLPSDPDRGVGRTHEISVRNSGSCLCLPRFMRLTFVPESLENLYQTYFKRQRHETLLVLVVFAALFDC YVVVMCAVVFSSDKLASLAVAGIGLVLDIILFVLCKKGLLPDRVTRRVLPYVLWLLITAQIFSYLGLNFARAHAASDTVGWQVFFVFSFF ITLPLSLSPIVIISVVSCVVHTLVLGVTVAQQQQEELKGMQLLREDGPGFYTTRCLAPMMSEVIRILQEGVDPKKLDSLTTSFGFPVGAA TLVDEVGVDVAKHVAEDLGKVFGERFGGGNPELLTQMVSKGFLGRKSGKGFYIYQEGVKRKDLNSDMDSILASLKLPPKSEVSSDEDIQF -------------------------------------------------------------- |
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Fusion Protein Functional Features |
| Four levels of functional features of fusion genes Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr2:25141182/chr2:26417507) - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. - How to search 1. Put your fusion gene symbol. 2. Press the tab key until there will be shown the breakpoint information filled. 4. Go down and press 'Search' tab twice. 4. Go down to have the hyperlink of the search result. 5. Click the hyperlink. 6. See the FGviewer result for your fusion gene. |
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| Main function of each fusion partner protein. (from UniProt) |
| Hgene | Tgene |
| ADCY3 | HADHA |
| FUNCTION: Catalyzes the formation of the signaling molecule cAMP in response to G-protein signaling. Participates in signaling cascades triggered by odorant receptors via its function in cAMP biosynthesis. Required for the perception of odorants. Required for normal sperm motility and normal male fertility. Plays a role in regulating insulin levels and body fat accumulation in response to a high fat diet. {ECO:0000250|UniProtKB:Q8VHH7}. | FUNCTION: Mitochondrial trifunctional enzyme catalyzes the last three of the four reactions of the mitochondrial beta-oxidation pathway (PubMed:8135828, PubMed:1550553, PubMed:29915090, PubMed:30850536). The mitochondrial beta-oxidation pathway is the major energy-producing process in tissues and is performed through four consecutive reactions breaking down fatty acids into acetyl-CoA (PubMed:29915090). Among the enzymes involved in this pathway, the trifunctional enzyme exhibits specificity for long-chain fatty acids (PubMed:30850536). Mitochondrial trifunctional enzyme is a heterotetrameric complex composed of two proteins, the trifunctional enzyme subunit alpha/HADHA described here carries the 2,3-enoyl-CoA hydratase and the 3-hydroxyacyl-CoA dehydrogenase activities while the trifunctional enzyme subunit beta/HADHB bears the 3-ketoacyl-CoA thiolase activity (PubMed:8135828, PubMed:29915090, PubMed:30850536). Independently of the subunit beta, the trifunctional enzyme subunit alpha/HADHA also has a monolysocardiolipin acyltransferase activity (PubMed:23152787). It acylates monolysocardiolipin into cardiolipin, a major mitochondrial membrane phospholipid which plays a key role in apoptosis and supports mitochondrial respiratory chain complexes in the generation of ATP (PubMed:23152787). Allows the acylation of monolysocardiolipin with different acyl-CoA substrates including oleoyl-CoA for which it displays the highest activity (PubMed:23152787). {ECO:0000269|PubMed:1550553, ECO:0000269|PubMed:23152787, ECO:0000269|PubMed:29915090, ECO:0000269|PubMed:30850536, ECO:0000269|PubMed:8135828, ECO:0000303|PubMed:29915090, ECO:0000303|PubMed:30850536}. |
| Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at * Minus value of BPloci means that the break pointn is located before the CDS. |
| - Retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| Hgene | ADCY3 | chr2:25141182 | chr2:26417507 | ENST00000260600 | - | 1 | 21 | 1_79 | 225.0 | 1145.0 | Topological domain | Cytoplasmic |
| Hgene | ADCY3 | chr2:25141182 | chr2:26417507 | ENST00000260600 | - | 1 | 21 | 105_125 | 225.0 | 1145.0 | Transmembrane | Helical |
| Hgene | ADCY3 | chr2:25141182 | chr2:26417507 | ENST00000260600 | - | 1 | 21 | 139_159 | 225.0 | 1145.0 | Transmembrane | Helical |
| Hgene | ADCY3 | chr2:25141182 | chr2:26417507 | ENST00000260600 | - | 1 | 21 | 173_193 | 225.0 | 1145.0 | Transmembrane | Helical |
| Hgene | ADCY3 | chr2:25141182 | chr2:26417507 | ENST00000260600 | - | 1 | 21 | 80_100 | 225.0 | 1145.0 | Transmembrane | Helical |
| - Not-retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| Hgene | ADCY3 | chr2:25141182 | chr2:26417507 | ENST00000260600 | - | 1 | 21 | 1062_1064 | 225.0 | 1145.0 | Nucleotide binding | ATP |
| Hgene | ADCY3 | chr2:25141182 | chr2:26417507 | ENST00000260600 | - | 1 | 21 | 1069_1073 | 225.0 | 1145.0 | Nucleotide binding | ATP |
| Hgene | ADCY3 | chr2:25141182 | chr2:26417507 | ENST00000260600 | - | 1 | 21 | 324_329 | 225.0 | 1145.0 | Nucleotide binding | ATP |
| Hgene | ADCY3 | chr2:25141182 | chr2:26417507 | ENST00000260600 | - | 1 | 21 | 366_368 | 225.0 | 1145.0 | Nucleotide binding | ATP |
| Hgene | ADCY3 | chr2:25141182 | chr2:26417507 | ENST00000260600 | - | 1 | 21 | 402_632 | 225.0 | 1145.0 | Topological domain | Cytoplasmic |
| Hgene | ADCY3 | chr2:25141182 | chr2:26417507 | ENST00000260600 | - | 1 | 21 | 854_1144 | 225.0 | 1145.0 | Topological domain | Cytoplasmic |
| Hgene | ADCY3 | chr2:25141182 | chr2:26417507 | ENST00000260600 | - | 1 | 21 | 226_246 | 225.0 | 1145.0 | Transmembrane | Helical |
| Hgene | ADCY3 | chr2:25141182 | chr2:26417507 | ENST00000260600 | - | 1 | 21 | 381_401 | 225.0 | 1145.0 | Transmembrane | Helical |
| Hgene | ADCY3 | chr2:25141182 | chr2:26417507 | ENST00000260600 | - | 1 | 21 | 633_653 | 225.0 | 1145.0 | Transmembrane | Helical |
| Hgene | ADCY3 | chr2:25141182 | chr2:26417507 | ENST00000260600 | - | 1 | 21 | 664_684 | 225.0 | 1145.0 | Transmembrane | Helical |
| Hgene | ADCY3 | chr2:25141182 | chr2:26417507 | ENST00000260600 | - | 1 | 21 | 708_728 | 225.0 | 1145.0 | Transmembrane | Helical |
| Hgene | ADCY3 | chr2:25141182 | chr2:26417507 | ENST00000260600 | - | 1 | 21 | 754_774 | 225.0 | 1145.0 | Transmembrane | Helical |
| Hgene | ADCY3 | chr2:25141182 | chr2:26417507 | ENST00000260600 | - | 1 | 21 | 775_795 | 225.0 | 1145.0 | Transmembrane | Helical |
| Hgene | ADCY3 | chr2:25141182 | chr2:26417507 | ENST00000260600 | - | 1 | 21 | 833_853 | 225.0 | 1145.0 | Transmembrane | Helical |
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Fusion Protein-Protein Interaction |
| Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in |
| Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160) |
| Gene | PPI interactors |
| Protein-protein interactors based on sequence similarity (STRING) |
| Gene | STRING network |
| ADCY3 | |
| HADHA |
| - Retained interactions in fusion protein (protein functional feature from UniProt). |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Still interaction with |
| - Lost interactions due to fusion (protein functional feature from UniProt). |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
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Related Drugs to ADCY3-HADHA |
| Drugs used for this fusion-positive patient. (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
| Hgene | Tgene | Drug | Source | PMID |
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Related Diseases to ADCY3-HADHA |
| Diseases that have this fusion gene. (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
| Hgene | Tgene | Disease | Source | PMID |
| Diseases associated with fusion partners. (DisGeNet 4.0) |
| Partner | Gene | Disease ID | Disease name | # pubmeds | Source |
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