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	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein Structure
	pLDDT scores
	Ramachandran Plot of Fusion Protein Structure
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:DHX9-PRELID1

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: DHX9-PRELID1
	FusionPDB ID: 22730
	FusionGDB2.0 ID: 22730
		Hgene	Tgene
	Gene symbol	DHX9	PRELID1
	Gene ID	1660	27166
	Gene name	DExH-box helicase 9	PRELI domain containing 1
	Synonyms	DDX9\|LKP\|NDH2\|NDHII\|RHA	CGI-106\|PRELI\|PX19\|SBBI12
	Cytomap	1q25.3	5q35.3
	Type of gene	protein-coding	protein-coding
	Description	ATP-dependent RNA helicase ADEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 9DEAH (Asp-Glu-Ala-His) box helicase 9DEAH (Asp-Glu-Ala-His) box polypeptide 9DEAH box protein 9DEAH-box helicase 9RNA helicase Aleukophysinnuclear DNA helicase II	PRELI domain-containing protein 1, mitochondrial25 kDa protein of relevant evolutionary and lymphoid interestpx19-like protein
	Modification date	20200322	20200313
	UniProtAcc	Q08211	.
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000485081, ENST00000367549,	ENST00000303204, ENST00000502670, ENST00000503216,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	14 X 10 X 7=980	3 X 4 X 2=24
	# samples	15	4
	** MAII score	log2(15/980*10)=-2.70781924850669 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(4/24*10)=0.736965594166206 effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs). DoF>8 and MAII>0
Context (manual curation of fusion genes in FusionPDB)	PubMed: DHX9 [Title/Abstract] AND PRELID1 [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	DHX9(182850782)-PRELID1(176733423), # samples:3
Anticipated loss of major functional domain due to fusion event.	DHX9-PRELID1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. DHX9-PRELID1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. DHX9-PRELID1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. DHX9-PRELID1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	DHX9	GO:0010501	RNA secondary structure unwinding	1537828\|9111062
Hgene	DHX9	GO:0032508	DNA duplex unwinding	9111062\|21561811
Hgene	DHX9	GO:0034622	cellular protein-containing complex assembly	23361462
Hgene	DHX9	GO:0039695	DNA-templated viral transcription	11096080
Hgene	DHX9	GO:0044806	G-quadruplex DNA unwinding	21561811
Hgene	DHX9	GO:0046833	positive regulation of RNA export from nucleus	11402034
Hgene	DHX9	GO:0050434	positive regulation of viral transcription	11096080
Hgene	DHX9	GO:0050684	regulation of mRNA processing	28355180
Hgene	DHX9	GO:2000765	regulation of cytoplasmic translation	28355180
Tgene	PRELID1	GO:0010917	negative regulation of mitochondrial membrane potential	18945965
Tgene	PRELID1	GO:0010950	positive regulation of endopeptidase activity	18945965
Tgene	PRELID1	GO:0043066	negative regulation of apoptotic process	21364629
Tgene	PRELID1	GO:0045580	regulation of T cell differentiation	18945965
Tgene	PRELID1	GO:0051881	regulation of mitochondrial membrane potential	21364629
Tgene	PRELID1	GO:0070234	positive regulation of T cell apoptotic process	18945965
Tgene	PRELID1	GO:0090201	negative regulation of release of cytochrome c from mitochondria	23931759
Tgene	PRELID1	GO:0097035	regulation of membrane lipid distribution	23931759
Tgene	PRELID1	GO:1901857	positive regulation of cellular respiration	21364629\|23931759
Tgene	PRELID1	GO:2001140	positive regulation of phospholipid transport	23931759

Fusion gene breakpoints across DHX9 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across PRELID1 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	PRAD	TCGA-TK-A8OK-01A	DHX9	chr1	182850782	-	PRELID1	chr5	176733423	+
ChimerDB4	PRAD	TCGA-TK-A8OK-01A	DHX9	chr1	182850782	+	PRELID1	chr5	176733423	+
ChimerDB4	PRAD	TCGA-TK-A8OK	DHX9	chr1	182850782	+	PRELID1	chr5	176733422	+
ChimerDB4	PRAD	TCGA-TK-A8OK	DHX9	chr1	182850782	+	PRELID1	chr5	176733423	+

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst

Tenst

Hgene

Hchr

Hbp

Hstrand

Tgene

Tchr

Tbp

Tstrand

Seq length
(transcript)

BP loci
(transcript)

Predicted start
(transcript)

Predicted stop
(transcript)

Seq length
(amino acids)

ENST00000367549

DHX9

chr1

182850782

ENST00000303204

PRELID1

chr5

176733423

3562

3024

110

3172

1020

ENST00000367549

DHX9

chr1

182850782

ENST00000303204

PRELID1

chr5

176733422

3562

3024

110

3172

1020

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000367549	ENST00000303204	DHX9	chr1	182850782	+	PRELID1	chr5	176733423	+	0.001244406	0.9987556
ENST00000367549	ENST00000303204	DHX9	chr1	182850782	+	PRELID1	chr5	176733422	+	0.001244406	0.9987556

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>22730_22730_1_DHX9-PRELID1_DHX9_chr1_182850782_ENST00000367549_PRELID1_chr5_176733422_ENST00000303204_length(amino acids)=1020AA_BP=971
MGDVKNFLYAWCGKRKMTPSYEIRAVGNKNRQKFMCEVQVEGYNYTGMGNSTNKKDAQSNAARDFVNYLVRINEIKSEEVPAFGVASPPP
LTDTPDTTANAEGDLPTTMGGPLPPHLALKAENNSEVGASGYGVPGPTWDRGANLKDYYSRKEEQEVQATLESEEVDLNAGLHGNWTLEN
AKARLNQYFQKEKIQGEYKYTQVGPDHNRSFIAEMTIYIKQLGRRIFAREHGSNKKLAAQSCALSLVRQLYHLGVVEAYSGLTKKKEGET
VEPYKVNLSQDLEHQLQNIIQELNLEILPPPEDPSVPVALNIGKLAQFEPSQRQNQVGVVPWSPPQSNWNPWTSSNIDEGPLAFATPEQI
SMDLKNELMYQLEQDHDLQAILQERELLPVKKFESEILEAISQNSVVIIRGATGCGKTTQVPQFILDDFIQNDRAAECNIVVTQPRRISA
VSVAERVAFERGEEPGKSCGYSVRFESILPRPHASIMFCTVGVLLRKLEAGIRGISHVIVDEIHERDINTDFLLVVLRDVVQAYPEVRIV
LMSATIDTSMFCEYFFNCPIIEVYGRTYPVQEYFLEDCIQMTHFVPPPKDKKKKDKDDDGGEDDDANCNLICGDEYGPETRLSMSQLNEK
ETPFELIEALLKYIETLNVPGAVLVFLPGWNLIYTMQKHLEMNPHFGSHRYQILPLHSQIPREEQRKVFDPVPVGVTKVILSTNIAETSI
TINDVVYVIDSCKQKVKLFTAHNNMTNYATVWASKTNLEQRKGRAGRVRPGFCFHLCSRARFERLETHMTPEMFRTPLHEIALSIKLLRL
GGIGQFLAKAIEPPPLDAVIEAEHTLRELDALDANDELTPLGRILAKLPIEPRFGKMMIMGCIFYVGDAICTIAAATCFPEPFINEGKRL
GYIHRNFAGNRFSDHVALLSVFQAWDDARMGGEEAEIRFCEHKRLNMATLRMTWEAKVQLKEILINSGFPEGEAPSKTLVETAKEAKEKA

--------------------------------------------------------------

>22730_22730_2_DHX9-PRELID1_DHX9_chr1_182850782_ENST00000367549_PRELID1_chr5_176733423_ENST00000303204_length(amino acids)=1020AA_BP=971
MGDVKNFLYAWCGKRKMTPSYEIRAVGNKNRQKFMCEVQVEGYNYTGMGNSTNKKDAQSNAARDFVNYLVRINEIKSEEVPAFGVASPPP
LTDTPDTTANAEGDLPTTMGGPLPPHLALKAENNSEVGASGYGVPGPTWDRGANLKDYYSRKEEQEVQATLESEEVDLNAGLHGNWTLEN
AKARLNQYFQKEKIQGEYKYTQVGPDHNRSFIAEMTIYIKQLGRRIFAREHGSNKKLAAQSCALSLVRQLYHLGVVEAYSGLTKKKEGET
VEPYKVNLSQDLEHQLQNIIQELNLEILPPPEDPSVPVALNIGKLAQFEPSQRQNQVGVVPWSPPQSNWNPWTSSNIDEGPLAFATPEQI
SMDLKNELMYQLEQDHDLQAILQERELLPVKKFESEILEAISQNSVVIIRGATGCGKTTQVPQFILDDFIQNDRAAECNIVVTQPRRISA
VSVAERVAFERGEEPGKSCGYSVRFESILPRPHASIMFCTVGVLLRKLEAGIRGISHVIVDEIHERDINTDFLLVVLRDVVQAYPEVRIV
LMSATIDTSMFCEYFFNCPIIEVYGRTYPVQEYFLEDCIQMTHFVPPPKDKKKKDKDDDGGEDDDANCNLICGDEYGPETRLSMSQLNEK
ETPFELIEALLKYIETLNVPGAVLVFLPGWNLIYTMQKHLEMNPHFGSHRYQILPLHSQIPREEQRKVFDPVPVGVTKVILSTNIAETSI
TINDVVYVIDSCKQKVKLFTAHNNMTNYATVWASKTNLEQRKGRAGRVRPGFCFHLCSRARFERLETHMTPEMFRTPLHEIALSIKLLRL
GGIGQFLAKAIEPPPLDAVIEAEHTLRELDALDANDELTPLGRILAKLPIEPRFGKMMIMGCIFYVGDAICTIAAATCFPEPFINEGKRL
GYIHRNFAGNRFSDHVALLSVFQAWDDARMGGEEAEIRFCEHKRLNMATLRMTWEAKVQLKEILINSGFPEGEAPSKTLVETAKEAKEKA

--------------------------------------------------------------

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Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr1:182850782/chr5:176733423)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
DHX9 Q08211	.
FUNCTION: Multifunctional ATP-dependent nucleic acid helicase that unwinds DNA and RNA in a 3' to 5' direction and that plays important roles in many processes, such as DNA replication, transcriptional activation, post-transcriptional RNA regulation, mRNA translation and RNA-mediated gene silencing (PubMed:9111062, PubMed:11416126, PubMed:12711669, PubMed:15355351, PubMed:16680162, PubMed:17531811, PubMed:20669935, PubMed:21561811, PubMed:24049074, PubMed:25062910, PubMed:24990949, PubMed:28221134). Requires a 3'-single-stranded tail as entry site for acid nuclei unwinding activities as well as the binding and hydrolyzing of any of the four ribo- or deoxyribo-nucleotide triphosphates (NTPs) (PubMed:1537828). Unwinds numerous nucleic acid substrates such as double-stranded (ds) DNA and RNA, DNA:RNA hybrids, DNA and RNA forks composed of either partially complementary DNA duplexes or DNA:RNA hybrids, respectively, and also DNA and RNA displacement loops (D- and R-loops), triplex-helical DNA (H-DNA) structure and DNA and RNA-based G-quadruplexes (PubMed:20669935, PubMed:21561811, PubMed:24049074). Binds dsDNA, single-stranded DNA (ssDNA), dsRNA, ssRNA and poly(A)-containing RNA (PubMed:9111062, PubMed:10198287). Binds also to circular dsDNA or dsRNA of either linear and/or circular forms and stimulates the relaxation of supercoiled DNAs catalyzed by topoisomerase TOP2A (PubMed:12711669). Plays a role in DNA replication at origins of replication and cell cycle progression (PubMed:24990949). Plays a role as a transcriptional coactivator acting as a bridging factor between polymerase II holoenzyme and transcription factors or cofactors, such as BRCA1, CREBBP, RELA and SMN1 (PubMed:11149922, PubMed:9323138, PubMed:9662397, PubMed:11038348, PubMed:11416126, PubMed:15355351, PubMed:28221134). Binds to the CDKN2A promoter (PubMed:11038348). Plays several roles in post-transcriptional regulation of gene expression (PubMed:28221134, PubMed:28355180). In cooperation with NUP98, promotes pre-mRNA alternative splicing activities of a subset of genes (PubMed:11402034, PubMed:16680162, PubMed:28221134, PubMed:28355180). As component of a large PER complex, is involved in the negative regulation of 3' transcriptional termination of circadian target genes such as PER1 and NR1D1 and the control of the circadian rhythms (By similarity). Acts also as a nuclear resolvase that is able to bind and neutralize harmful massive secondary double-stranded RNA structures formed by inverted-repeat Alu retrotransposon elements that are inserted and transcribed as parts of genes during the process of gene transposition (PubMed:28355180). Involved in the positive regulation of nuclear export of constitutive transport element (CTE)-containing unspliced mRNA (PubMed:9162007, PubMed:10924507, PubMed:11402034). Component of the coding region determinant (CRD)-mediated complex that promotes cytoplasmic MYC mRNA stability (PubMed:19029303). Plays a role in mRNA translation (PubMed:28355180). Positively regulates translation of selected mRNAs through its binding to post-transcriptional control element (PCE) in the 5'-untranslated region (UTR) (PubMed:16680162). Involved with LARP6 in the translation stimulation of type I collagen mRNAs for CO1A1 and CO1A2 through binding of a specific stem-loop structure in their 5'-UTRs (PubMed:22190748). Stimulates LIN28A-dependent mRNA translation probably by facilitating ribonucleoprotein remodeling during the process of translation (PubMed:21247876). Plays also a role as a small interfering (siRNA)-loading factor involved in the RNA-induced silencing complex (RISC) loading complex (RLC) assembly, and hence functions in the RISC-mediated gene silencing process (PubMed:17531811). Binds preferentially to short double-stranded RNA, such as those produced during rotavirus intestinal infection (PubMed:28636595). This interaction may mediate NLRP9 inflammasome activation and trigger inflammatory response, including IL18 release and pyroptosis (PubMed:28636595). Finally, mediates the attachment of heterogeneous nuclear ribonucleoproteins (hnRNPs) to actin filaments in the nucleus (PubMed:11687588). {ECO:0000250\|UniProtKB:O70133, ECO:0000269\|PubMed:10198287, ECO:0000269\|PubMed:10924507, ECO:0000269\|PubMed:11038348, ECO:0000269\|PubMed:11149922, ECO:0000269\|PubMed:11402034, ECO:0000269\|PubMed:11416126, ECO:0000269\|PubMed:11687588, ECO:0000269\|PubMed:12711669, ECO:0000269\|PubMed:15355351, ECO:0000269\|PubMed:1537828, ECO:0000269\|PubMed:16680162, ECO:0000269\|PubMed:17531811, ECO:0000269\|PubMed:19029303, ECO:0000269\|PubMed:20669935, ECO:0000269\|PubMed:21247876, ECO:0000269\|PubMed:21561811, ECO:0000269\|PubMed:22190748, ECO:0000269\|PubMed:24049074, ECO:0000269\|PubMed:24990949, ECO:0000269\|PubMed:25062910, ECO:0000269\|PubMed:28221134, ECO:0000269\|PubMed:28355180, ECO:0000269\|PubMed:28636595, ECO:0000269\|PubMed:9111062, ECO:0000269\|PubMed:9162007, ECO:0000269\|PubMed:9323138, ECO:0000269\|PubMed:9662397}.; FUNCTION: (Microbial infection) Plays a role in HIV-1 replication and virion infectivity (PubMed:11096080, PubMed:19229320, PubMed:25149208, PubMed:27107641). Enhances HIV-1 transcription by facilitating the binding of RNA polymerase II holoenzyme to the proviral DNA (PubMed:11096080, PubMed:25149208). Binds (via DRBM domain 2) to the HIV-1 TAR RNA and stimulates HIV-1 transcription of transactivation response element (TAR)-containing mRNAs (PubMed:9892698, PubMed:11096080). Involved also in HIV-1 mRNA splicing and transport (PubMed:25149208). Positively regulates HIV-1 gag mRNA translation, through its binding to post-transcriptional control element (PCE) in the 5'-untranslated region (UTR) (PubMed:16680162). Binds (via DRBM domains) to a HIV-1 double-stranded RNA region of the primer binding site (PBS)-segment of the 5'-UTR, and hence stimulates DHX9 incorporation into virions and virion infectivity (PubMed:27107641). Plays also a role as a cytosolic viral MyD88-dependent DNA and RNA sensors in plasmacytoid dendritic cells (pDCs), and hence induce antiviral innate immune responses (PubMed:20696886, PubMed:21957149). Binds (via the OB-fold region) to viral single-stranded DNA unmethylated C-phosphate-G (CpG) oligonucleotide (PubMed:20696886). {ECO:0000269\|PubMed:11096080, ECO:0000269\|PubMed:16680162, ECO:0000269\|PubMed:19229320, ECO:0000269\|PubMed:20696886, ECO:0000269\|PubMed:21957149, ECO:0000269\|PubMed:25149208, ECO:0000269\|PubMed:27107641, ECO:0000269\|PubMed:9892698}.	FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

DHX9

chr1:182850782

chr5:176733422

ENST00000367549

180_252

971.3333333333334

1271.0

Domain

DRBM 2

Hgene

DHX9

chr1:182850782

chr5:176733422

ENST00000367549

398_564

971.3333333333334

1271.0

Domain

Helicase ATP-binding

Hgene

DHX9

chr1:182850782

chr5:176733422

ENST00000367549

3_71

971.3333333333334

1271.0

Domain

DRBM 1

Hgene

DHX9

chr1:182850782

chr5:176733422

ENST00000367549

636_809

971.3333333333334

1271.0

Domain

Helicase C-terminal

Hgene

DHX9

chr1:182850782

chr5:176733423

ENST00000367549

180_252

971.3333333333334

1271.0

Domain

DRBM 2

Hgene

DHX9

chr1:182850782

chr5:176733423

ENST00000367549

398_564

971.3333333333334

1271.0

Domain

Helicase ATP-binding

Hgene

DHX9

chr1:182850782

chr5:176733423

ENST00000367549

3_71

971.3333333333334

1271.0

Domain

DRBM 1

Hgene

DHX9

chr1:182850782

chr5:176733423

ENST00000367549

636_809

971.3333333333334

1271.0

Domain

Helicase C-terminal

Hgene

DHX9

chr1:182850782

chr5:176733422

ENST00000367549

511_514

971.3333333333334

1271.0

Motif

Note=DEIH box

Hgene

DHX9

chr1:182850782

chr5:176733422

ENST00000367549

586_595

971.3333333333334

1271.0

Motif

Nuclear localization signal (NLS1)

Hgene

DHX9

chr1:182850782

chr5:176733423

ENST00000367549

511_514

971.3333333333334

1271.0

Motif

Note=DEIH box

Hgene

DHX9

chr1:182850782

chr5:176733423

ENST00000367549

586_595

971.3333333333334

1271.0

Motif

Nuclear localization signal (NLS1)

Hgene

DHX9

chr1:182850782

chr5:176733422

ENST00000367549

411_419

971.3333333333334

1271.0

Nucleotide binding

ATP

Hgene

DHX9

chr1:182850782

chr5:176733423

ENST00000367549

411_419

971.3333333333334

1271.0

Nucleotide binding

ATP

Hgene

DHX9

chr1:182850782

chr5:176733422

ENST00000367549

182_186

971.3333333333334

1271.0

Region

siRNA-binding

Hgene

DHX9

chr1:182850782

chr5:176733422

ENST00000367549

234_236

971.3333333333334

1271.0

Region

siRNA-binding

Hgene

DHX9

chr1:182850782

chr5:176733422

ENST00000367549

331_380

971.3333333333334

1271.0

Region

MTAD

Hgene

DHX9

chr1:182850782

chr5:176733422

ENST00000367549

398_809

971.3333333333334

1271.0

Region

Core helicase

Hgene

DHX9

chr1:182850782

chr5:176733422

ENST00000367549

53_55

971.3333333333334

1271.0

Region

siRNA-binding

Hgene

DHX9

chr1:182850782

chr5:176733422

ENST00000367549

5_9

971.3333333333334

1271.0

Region

siRNA-binding

Hgene

DHX9

chr1:182850782

chr5:176733422

ENST00000367549

831_919

971.3333333333334

1271.0

Region

HA2

Hgene

DHX9

chr1:182850782

chr5:176733423

ENST00000367549

182_186

971.3333333333334

1271.0

Region

siRNA-binding

Hgene

DHX9

chr1:182850782

chr5:176733423

ENST00000367549

234_236

971.3333333333334

1271.0

Region

siRNA-binding

Hgene

DHX9

chr1:182850782

chr5:176733423

ENST00000367549

331_380

971.3333333333334

1271.0

Region

MTAD

Hgene

DHX9

chr1:182850782

chr5:176733423

ENST00000367549

398_809

971.3333333333334

1271.0

Region

Core helicase

Hgene

DHX9

chr1:182850782

chr5:176733423

ENST00000367549

53_55

971.3333333333334

1271.0

Region

siRNA-binding

Hgene

DHX9

chr1:182850782

chr5:176733423

ENST00000367549

5_9

971.3333333333334

1271.0

Region

siRNA-binding

Hgene

DHX9

chr1:182850782

chr5:176733423

ENST00000367549

831_919

971.3333333333334

1271.0

Region

HA2

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

DHX9

chr1:182850782

chr5:176733422

ENST00000367549

1155_1173

971.3333333333334

1271.0

Motif

Nuclear localization signal (NLS2)

Hgene

DHX9

chr1:182850782

chr5:176733423

ENST00000367549

1155_1173

971.3333333333334

1271.0

Motif

Nuclear localization signal (NLS2)

Hgene

DHX9

chr1:182850782

chr5:176733422

ENST00000367549

1150_1270

971.3333333333334

1271.0

Region

RGG

Hgene

DHX9

chr1:182850782

chr5:176733422

ENST00000367549

958_1074

971.3333333333334

1271.0

Region

OB-fold

Hgene

DHX9

chr1:182850782

chr5:176733423

ENST00000367549

1150_1270

971.3333333333334

1271.0

Region

RGG

Hgene

DHX9

chr1:182850782

chr5:176733423

ENST00000367549

958_1074

971.3333333333334

1271.0

Region

OB-fold

Tgene

PRELID1

chr1:182850782

chr5:176733422

ENST00000303204

36_174

170.33333333333334

220.0

Domain

PRELI/MSF1

Tgene

PRELID1

chr1:182850782

chr5:176733423

ENST00000303204

36_174

170.33333333333334

220.0

Domain

PRELI/MSF1

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Fusion Protein Structures

PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.

Fusion protein PDB link (fusion AA seq ID in FusionPDB)	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	AA seq	Len(AA seq)
PDB file >>>1800_DHX9_182850782_PRELID1_176733423_ranked_0.pdb	DHX9	182850782	182850782	ENST00000303204	PRELID1	chr5	176733423	+	MGDVKNFLYAWCGKRKMTPSYEIRAVGNKNRQKFMCEVQVEGYNYTGMGNSTNKKDAQSNAARDFVNYLVRINEIKSEEVPAFGVASPPP LTDTPDTTANAEGDLPTTMGGPLPPHLALKAENNSEVGASGYGVPGPTWDRGANLKDYYSRKEEQEVQATLESEEVDLNAGLHGNWTLEN AKARLNQYFQKEKIQGEYKYTQVGPDHNRSFIAEMTIYIKQLGRRIFAREHGSNKKLAAQSCALSLVRQLYHLGVVEAYSGLTKKKEGET VEPYKVNLSQDLEHQLQNIIQELNLEILPPPEDPSVPVALNIGKLAQFEPSQRQNQVGVVPWSPPQSNWNPWTSSNIDEGPLAFATPEQI SMDLKNELMYQLEQDHDLQAILQERELLPVKKFESEILEAISQNSVVIIRGATGCGKTTQVPQFILDDFIQNDRAAECNIVVTQPRRISA VSVAERVAFERGEEPGKSCGYSVRFESILPRPHASIMFCTVGVLLRKLEAGIRGISHVIVDEIHERDINTDFLLVVLRDVVQAYPEVRIV LMSATIDTSMFCEYFFNCPIIEVYGRTYPVQEYFLEDCIQMTHFVPPPKDKKKKDKDDDGGEDDDANCNLICGDEYGPETRLSMSQLNEK ETPFELIEALLKYIETLNVPGAVLVFLPGWNLIYTMQKHLEMNPHFGSHRYQILPLHSQIPREEQRKVFDPVPVGVTKVILSTNIAETSI TINDVVYVIDSCKQKVKLFTAHNNMTNYATVWASKTNLEQRKGRAGRVRPGFCFHLCSRARFERLETHMTPEMFRTPLHEIALSIKLLRL GGIGQFLAKAIEPPPLDAVIEAEHTLRELDALDANDELTPLGRILAKLPIEPRFGKMMIMGCIFYVGDAICTIAAATCFPEPFINEGKRL GYIHRNFAGNRFSDHVALLSVFQAWDDARMGGEEAEIRFCEHKRLNMATLRMTWEAKVQLKEILINSGFPEGEAPSKTLVETAKEAKEKA	1020

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pLDDT score distribution

pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

DHX9_pLDDT.png

PRELID1_pLDDT.png

pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

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Ramachandran Plot of Fusion Protein Structure

Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.

Fusion AA seq ID in FusionPDB and their Ramachandran plots

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Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
DHX9
PRELID1

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

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Related Drugs to DHX9-PRELID1

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

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Related Diseases to DHX9-PRELID1

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source