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Center for Computational Systems Medicine level1
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Fusion Gene Summary

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Fusion Gene Sample Information

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Fusion ORF Analysis

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Fusion Amino Acid Sequences

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Fusion Protein Functional Features

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Fusion Protein-Protein Interaction

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Related drugs with this fusion protein

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Related disease with this fusion protein

Fusion Protein:DLEU1-STIM1

Fusion Protein Summary

check button Fusion gene summary
Fusion partner gene informationFusion gene name: DLEU1-STIM1
FusionPDB ID: 23004
FusionGDB2.0 ID: 23004
HgeneTgene
Gene symbol

DLEU1

STIM1

Gene ID

10301

6786

Gene namedeleted in lymphocytic leukemia 1stromal interaction molecule 1
SynonymsBCMS|BCMS1|DLB1|DLEU2|LEU1|LEU2|LINC00021|NCRNA00021|XTP6D11S4896E|GOK|IMD10|STRMK|TAM|TAM1
Cytomap

13q14.2-q14.3

11p15.4

Type of genencRNAprotein-coding
DescriptionB-cell neoplasia-associated gene with multiple splicingdeleted in lymphocytic leukemia 1 (non-protein coding)leukemia-associated protein 2long intergenic non-protein coding RNA 21stromal interaction molecule 1
Modification date2020031320200329
UniProtAcc

O43261

.
Ensembl transtripts involved in fusion geneENST idsENST00000490577, ENST00000378180, 
ENST00000300737, ENST00000527651, 
ENST00000527484, ENST00000533977, 
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)* DoF score14 X 13 X 4=72811 X 11 X 4=484
# samples 1412
** MAII scorelog2(14/728*10)=-2.37851162325373
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
log2(12/484*10)=-2.01197264166608
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)

PubMed: DLEU1 [Title/Abstract] AND STIM1 [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)DLEU1(50656693)-STIM1(4076753), # samples:1
Anticipated loss of major functional domain due to fusion event.DLEU1-STIM1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
DLEU1-STIM1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
DLEU1-STIM1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
DLEU1-STIM1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
TgeneSTIM1

GO:0002115

store-operated calcium entry

28219928

TgeneSTIM1

GO:0005513

detection of calcium ion

16005298

TgeneSTIM1

GO:0032237

activation of store-operated calcium channel activity

16005298|26322679|28219928

TgeneSTIM1

GO:0045762

positive regulation of adenylate cyclase activity

19171672

TgeneSTIM1

GO:0051924

regulation of calcium ion transport

16005298


check buttonFusion gene breakpoints across DLEU1 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check buttonFusion gene breakpoints across STIM1 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure


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Fusion Gene Sample Information

check buttonFusion gene information from FusionGDB2.0.
check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChiTaRS5.0N/ADB149054DLEU1chr13

50656693

+STIM1chr11

4076753

+


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Fusion ORF Analysis


check buttonFusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)
BP loci
(transcript)
Predicted start
(transcript)
Predicted stop
(transcript)
Seq length
(amino acids)
ENST00000378180DLEU1chr1350656693+ENST00000300737STIM1chr114076753+34713872662059597
ENST00000378180DLEU1chr1350656693+ENST00000527651STIM1chr114076753+20973872661624452

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000378180ENST00000300737DLEU1chr1350656693+STIM1chr114076753+0.0068073380.99319273
ENST00000378180ENST00000527651DLEU1chr1350656693+STIM1chr114076753+0.018517910.98148215

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Fusion Amino Acid Sequences


check button For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>23004_23004_1_DLEU1-STIM1_DLEU1_chr13_50656693_ENST00000378180_STIM1_chr11_4076753_ENST00000300737_length(amino acids)=597AA_BP=40
MRPCIWIHVHLKPPCRLVELLPFSSALQGLSHLSLGTTLPVYNWTVDEVVQWLITYVELPQYEETFRKLQLSGHAMPRLAVTNTTMTGTV
LKMTDRSHRQKLQLKALDTVLFGPPLLTRHNHLKDFMLVVSIVIGVGGCWFAYIQNRYSKEHMKKMMKDLEGLHRAEQSLHDLQERLHKA
QEEHRTVEVEKVHLEKKLRDEINLAKQEAQRLKELREGTENERSRQKYAEEELEQVREALRKAEKELESHSSWYAPEALQKWLQLTHEVE
VQYYNIKKQNAEKQLLVAKEGAEKIKKKRNTLFGTFHVAHSSSLDDVDHKILTAKQALSEVTAALRERLHRWQQIEILCGFQIVNNPGIH
SLVAALNIDPSWMGSTRPNPAHFIMTDDVDDMDEEIVSPLSMQSPSLQSSVRQRLTEPQHGLGSQRDLTHSDSESSLHMSDRQRVAPKPP
QMSRAADEALNAMTSNGSHRLIEGVHPGSLVEKLPDSPALAKKALLALNHGLDKAHSLMELSPSAPPGGSPHLDSSRSHSPSSPDPDTPS

--------------------------------------------------------------

>23004_23004_2_DLEU1-STIM1_DLEU1_chr13_50656693_ENST00000378180_STIM1_chr11_4076753_ENST00000527651_length(amino acids)=452AA_BP=40
MRPCIWIHVHLKPPCRLVELLPFSSALQGLSHLSLGTTLPVYNWTVDEVVQWLITYVELPQYEETFRKLQLSGHAMPRLAVTNTTMTGTV
LKMTDRSHRQKLQLKALDTVLFGPPLLTRHNHLKDFMLVVSIVIGVGGCWFAYIQNRYSKEHMKKMMKDLEGLHRAEQSLHDLQERLHKA
QEEHRTVEVEKVHLEKKLRDEINLAKQEAQRLKELREGTENERSRQKYAEEELEQVREALRKAEKELESHSSWYAPEALQKWLQLTHEVE
VQYYNIKKQNAEKQLLVAKEGAEKIKKKRNTLFGTFHVAHSSSLDDVDHKILTAKQALSEVTAALRERLHRWQQIEILCGFQIVNNPGIH
SLVAALNIDPSWMGSTRPNPAHFIMTDDVDDMDEEIVSPLSMQSPSLQSSVRQRLTEPQHGLGSQRGSSLKANRLSSKGFDPFRFGVLPP

--------------------------------------------------------------

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Fusion Protein Functional Features


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr13:50656693/chr11:4076753)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
DLEU1

O43261

.
FUNCTION: May act as a tumor suppressor.FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page

* Minus value of BPloci means that the break pointn is located before the CDS.
- Retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
TgeneSTIM1chr13:50656693chr11:4076753ENST00000300737212248_442128.33333333333334686.0Coiled coilOntology_term=ECO:0000269,ECO:0000269,ECO:0000269
TgeneSTIM1chr13:50656693chr11:4076753ENST00000300737212270_336128.33333333333334686.0Compositional biasNote=Glu-rich
TgeneSTIM1chr13:50656693chr11:4076753ENST00000300737212600_629128.33333333333334686.0Compositional biasNote=Pro/Ser-rich
TgeneSTIM1chr13:50656693chr11:4076753ENST00000300737212672_685128.33333333333334686.0Compositional biasNote=Lys-rich
TgeneSTIM1chr13:50656693chr11:4076753ENST00000300737212132_200128.33333333333334686.0DomainSAM
TgeneSTIM1chr13:50656693chr11:4076753ENST00000300737212642_645128.33333333333334686.0MotifNote=Microtubule tip localization signal
TgeneSTIM1chr13:50656693chr11:4076753ENST00000300737212334_444128.33333333333334686.0RegionNote=SOAR/CAD
TgeneSTIM1chr13:50656693chr11:4076753ENST00000300737212235_685128.33333333333334686.0Topological domainCytoplasmic
TgeneSTIM1chr13:50656693chr11:4076753ENST00000300737212214_234128.33333333333334686.0TransmembraneHelical

- Not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
TgeneSTIM1chr13:50656693chr11:4076753ENST0000030073721276_87128.33333333333334686.0Calcium binding.
TgeneSTIM1chr13:50656693chr11:4076753ENST0000030073721263_98128.33333333333334686.0DomainNote=EF-hand
TgeneSTIM1chr13:50656693chr11:4076753ENST0000030073721223_213128.33333333333334686.0Topological domainExtracellular


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Fusion Protein-Protein Interaction


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)
GenePPI interactors


check button Protein-protein interactors based on sequence similarity (STRING)
GeneSTRING network
DLEU1
STIM1


check button - Retained interactions in fusion protein (protein functional feature from UniProt).
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost interactions due to fusion (protein functional feature from UniProt).
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs to DLEU1-STIM1


check button Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDrugSourcePMID

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Related Diseases to DLEU1-STIM1


check button Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDiseaseSourcePMID

check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource