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	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein Structure
	pLDDT scores
	Ramachandran Plot of Fusion Protein Structure
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:DMTF1-SEMA3D

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: DMTF1-SEMA3D
	FusionPDB ID: 23264
	FusionGDB2.0 ID: 23264
		Hgene	Tgene
	Gene symbol	DMTF1	SEMA3D
	Gene ID	9988	223117
	Gene name	cyclin D binding myb like transcription factor 1	semaphorin 3D
	Synonyms	DMP1\|DMTF\|MRUL\|hDMP1	Sema-Z2\|coll-2
	Cytomap	7q21.12	7q21.11
	Type of gene	protein-coding	protein-coding
	Description	cyclin-D-binding Myb-like transcription factor 1cyclin D-binding Myb-like proteincyclin-D-interacting Myb-like protein 1hDMTF1	semaphorin-3Dcollapsin 2sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D
	Modification date	20200313	20200313
	UniProtAcc	Q9Y222	.
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000331242, ENST00000394702, ENST00000394703, ENST00000411766, ENST00000413276, ENST00000432937, ENST00000414194, ENST00000580710,	ENST00000484038, ENST00000284136, ENST00000444867,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	5 X 5 X 5=125	3 X 3 X 3=27
	# samples	5	3
	** MAII score	log2(5/125*10)=-1.32192809488736 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(3/27*10)=0.15200309344505 effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs). DoF>8 and MAII>0
Context (manual curation of fusion genes in FusionPDB)	PubMed: DMTF1 [Title/Abstract] AND SEMA3D [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	DMTF1(86800405)-SEMA3D(84702397), # samples:5
Anticipated loss of major functional domain due to fusion event.	DMTF1-SEMA3D seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. DMTF1-SEMA3D seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. DMTF1-SEMA3D seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. DMTF1-SEMA3D seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner

Gene

GO ID

GO term

PubMed ID

Fusion gene breakpoints across DMTF1 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across SEMA3D (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	LGG	TCGA-FG-5963-01A	DMTF1	chr7	86800405	-	SEMA3D	chr7	84702397	-
ChimerDB4	LGG	TCGA-FG-5963-01A	DMTF1	chr7	86800405	+	SEMA3D	chr7	84702397	-
ChimerDB4	LGG	TCGA-FG-5963-02A	DMTF1	chr7	86800405	-	SEMA3D	chr7	84702397	-
ChimerDB4	LGG	TCGA-FG-5963	DMTF1	chr7	86800405	+	SEMA3D	chr7	84702397	-

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000394702	DMTF1	chr7	86800405	+	ENST00000284136	SEMA3D	chr7	84702397	-	6499	653	320	2611	763
ENST00000394702	DMTF1	chr7	86800405	+	ENST00000444867	SEMA3D	chr7	84702397	-	1533	653	320	1162	280
ENST00000331242	DMTF1	chr7	86800405	+	ENST00000284136	SEMA3D	chr7	84702397	-	6499	653	320	2611	763
ENST00000331242	DMTF1	chr7	86800405	+	ENST00000444867	SEMA3D	chr7	84702397	-	1533	653	320	1162	280
ENST00000413276	DMTF1	chr7	86800405	+	ENST00000284136	SEMA3D	chr7	84702397	-	6451	605	272	2563	763
ENST00000413276	DMTF1	chr7	86800405	+	ENST00000444867	SEMA3D	chr7	84702397	-	1485	605	272	1114	280
ENST00000411766	DMTF1	chr7	86800405	+	ENST00000284136	SEMA3D	chr7	84702397	-	6311	465	255	2423	722
ENST00000411766	DMTF1	chr7	86800405	+	ENST00000444867	SEMA3D	chr7	84702397	-	1345	465	255	974	239
ENST00000432937	DMTF1	chr7	86800405	+	ENST00000284136	SEMA3D	chr7	84702397	-	6275	429	267	2387	706
ENST00000432937	DMTF1	chr7	86800405	+	ENST00000444867	SEMA3D	chr7	84702397	-	1309	429	267	938	223
ENST00000394703	DMTF1	chr7	86800405	+	ENST00000284136	SEMA3D	chr7	84702397	-	6736	890	557	2848	763
ENST00000394703	DMTF1	chr7	86800405	+	ENST00000444867	SEMA3D	chr7	84702397	-	1770	890	557	1399	280

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000394702	ENST00000284136	DMTF1	chr7	86800405	+	SEMA3D	chr7	84702397	-	0.000278656	0.99972135
ENST00000394702	ENST00000444867	DMTF1	chr7	86800405	+	SEMA3D	chr7	84702397	-	0.001792934	0.9982071
ENST00000331242	ENST00000284136	DMTF1	chr7	86800405	+	SEMA3D	chr7	84702397	-	0.000278656	0.99972135
ENST00000331242	ENST00000444867	DMTF1	chr7	86800405	+	SEMA3D	chr7	84702397	-	0.001792934	0.9982071
ENST00000413276	ENST00000284136	DMTF1	chr7	86800405	+	SEMA3D	chr7	84702397	-	0.000276513	0.9997235
ENST00000413276	ENST00000444867	DMTF1	chr7	86800405	+	SEMA3D	chr7	84702397	-	0.001715356	0.99828464
ENST00000411766	ENST00000284136	DMTF1	chr7	86800405	+	SEMA3D	chr7	84702397	-	0.00020522	0.9997948
ENST00000411766	ENST00000444867	DMTF1	chr7	86800405	+	SEMA3D	chr7	84702397	-	0.003506745	0.9964933
ENST00000432937	ENST00000284136	DMTF1	chr7	86800405	+	SEMA3D	chr7	84702397	-	0.000118785	0.99988115
ENST00000432937	ENST00000444867	DMTF1	chr7	86800405	+	SEMA3D	chr7	84702397	-	0.001370387	0.9986297
ENST00000394703	ENST00000284136	DMTF1	chr7	86800405	+	SEMA3D	chr7	84702397	-	0.000310376	0.9996897
ENST00000394703	ENST00000444867	DMTF1	chr7	86800405	+	SEMA3D	chr7	84702397	-	0.001888634	0.99811137

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>23264_23264_1_DMTF1-SEMA3D_DMTF1_chr7_86800405_ENST00000331242_SEMA3D_chr7_84702397_ENST00000284136_length(amino acids)=763AA_BP=110
MSMSTVEEDSDTVTVETVNSVTLTQDTEGNLILHCPQNEADEIDSEDSIEPPHKRLCLSSEDDQSIDDSTPCISVVALPLSENDQSFEVT
MTATTEVADDEVTEGTVTQIQTECANFIRVLQPYNKTHIYVCGTGAFHPICGYIDLGVYKEDIIFKLDTHNLESGRLKCPFDPQQPFASV
MTDEYLYSGTASDFLGKDTAFTRSLGPTHDHHYIRTDISEHYWLNGAKFIGTFFIPDTYNPDDDKIYFFFRESSQEGSTSDKTILSRVGR
VCKNDVGGQRSLINKWTTFLKARLICSIPGSDGADTYFDELQDIYLLPTRDERNPVVYGVFTTTSSIFKGSAVCVYSMADIRAVFNGPYA
HKESADHRWVQYDGRIPYPRPGTCPSKTYDPLIKSTRDFPDDVISFIKRHSVMYKSVYPVAGGPTFKRINVDYRLTQIVVDHVIAEDGQY
DVMFLGTDIGTVLKVVSISKEKWNMEEVVLEELQIFKHSSIILNMELSLKQQQLYIGSRDGLVQLSLHRCDTYGKACADCCLARDPYCAW
DGNACSRYAPTSKRRARRQDVKYGDPITQCWDIEDSISHETADEKVIFGIEFNSTFLECIPKSQQATIKWYIQRSGDEHREELKPDERII
KTEYGLLIRSLQKKDSGMYYCKAQEHTFIHTIVKLTLNVIENEQMENTQRAEHEEGKVKDLLAESRLRYKDYIQILSSPNFSLDQYCEQM

--------------------------------------------------------------

>23264_23264_2_DMTF1-SEMA3D_DMTF1_chr7_86800405_ENST00000331242_SEMA3D_chr7_84702397_ENST00000444867_length(amino acids)=280AA_BP=110
MSMSTVEEDSDTVTVETVNSVTLTQDTEGNLILHCPQNEADEIDSEDSIEPPHKRLCLSSEDDQSIDDSTPCISVVALPLSENDQSFEVT
MTATTEVADDEVTEGTVTQIQTECANFIRVLQPYNKTHIYVCGTGAFHPICGYIDLGVYKEDIIFKLDTHNLESGRLKCPFDPQQPFASV
MTDEYLYSGTASDFLGKDTAFTRSLGPTHDHHYIRTDISEHYWLNGAKFIGTFFIPDTYNPDDDKIYFFFRESSQEGSTSDKTILSRVGR

--------------------------------------------------------------

>23264_23264_3_DMTF1-SEMA3D_DMTF1_chr7_86800405_ENST00000394702_SEMA3D_chr7_84702397_ENST00000284136_length(amino acids)=763AA_BP=110
MSMSTVEEDSDTVTVETVNSVTLTQDTEGNLILHCPQNEADEIDSEDSIEPPHKRLCLSSEDDQSIDDSTPCISVVALPLSENDQSFEVT
MTATTEVADDEVTEGTVTQIQTECANFIRVLQPYNKTHIYVCGTGAFHPICGYIDLGVYKEDIIFKLDTHNLESGRLKCPFDPQQPFASV
MTDEYLYSGTASDFLGKDTAFTRSLGPTHDHHYIRTDISEHYWLNGAKFIGTFFIPDTYNPDDDKIYFFFRESSQEGSTSDKTILSRVGR
VCKNDVGGQRSLINKWTTFLKARLICSIPGSDGADTYFDELQDIYLLPTRDERNPVVYGVFTTTSSIFKGSAVCVYSMADIRAVFNGPYA
HKESADHRWVQYDGRIPYPRPGTCPSKTYDPLIKSTRDFPDDVISFIKRHSVMYKSVYPVAGGPTFKRINVDYRLTQIVVDHVIAEDGQY
DVMFLGTDIGTVLKVVSISKEKWNMEEVVLEELQIFKHSSIILNMELSLKQQQLYIGSRDGLVQLSLHRCDTYGKACADCCLARDPYCAW
DGNACSRYAPTSKRRARRQDVKYGDPITQCWDIEDSISHETADEKVIFGIEFNSTFLECIPKSQQATIKWYIQRSGDEHREELKPDERII
KTEYGLLIRSLQKKDSGMYYCKAQEHTFIHTIVKLTLNVIENEQMENTQRAEHEEGKVKDLLAESRLRYKDYIQILSSPNFSLDQYCEQM

--------------------------------------------------------------

>23264_23264_4_DMTF1-SEMA3D_DMTF1_chr7_86800405_ENST00000394702_SEMA3D_chr7_84702397_ENST00000444867_length(amino acids)=280AA_BP=110
MSMSTVEEDSDTVTVETVNSVTLTQDTEGNLILHCPQNEADEIDSEDSIEPPHKRLCLSSEDDQSIDDSTPCISVVALPLSENDQSFEVT
MTATTEVADDEVTEGTVTQIQTECANFIRVLQPYNKTHIYVCGTGAFHPICGYIDLGVYKEDIIFKLDTHNLESGRLKCPFDPQQPFASV
MTDEYLYSGTASDFLGKDTAFTRSLGPTHDHHYIRTDISEHYWLNGAKFIGTFFIPDTYNPDDDKIYFFFRESSQEGSTSDKTILSRVGR

--------------------------------------------------------------

>23264_23264_5_DMTF1-SEMA3D_DMTF1_chr7_86800405_ENST00000394703_SEMA3D_chr7_84702397_ENST00000284136_length(amino acids)=763AA_BP=110
MSMSTVEEDSDTVTVETVNSVTLTQDTEGNLILHCPQNEADEIDSEDSIEPPHKRLCLSSEDDQSIDDSTPCISVVALPLSENDQSFEVT
MTATTEVADDEVTEGTVTQIQTECANFIRVLQPYNKTHIYVCGTGAFHPICGYIDLGVYKEDIIFKLDTHNLESGRLKCPFDPQQPFASV
MTDEYLYSGTASDFLGKDTAFTRSLGPTHDHHYIRTDISEHYWLNGAKFIGTFFIPDTYNPDDDKIYFFFRESSQEGSTSDKTILSRVGR
VCKNDVGGQRSLINKWTTFLKARLICSIPGSDGADTYFDELQDIYLLPTRDERNPVVYGVFTTTSSIFKGSAVCVYSMADIRAVFNGPYA
HKESADHRWVQYDGRIPYPRPGTCPSKTYDPLIKSTRDFPDDVISFIKRHSVMYKSVYPVAGGPTFKRINVDYRLTQIVVDHVIAEDGQY
DVMFLGTDIGTVLKVVSISKEKWNMEEVVLEELQIFKHSSIILNMELSLKQQQLYIGSRDGLVQLSLHRCDTYGKACADCCLARDPYCAW
DGNACSRYAPTSKRRARRQDVKYGDPITQCWDIEDSISHETADEKVIFGIEFNSTFLECIPKSQQATIKWYIQRSGDEHREELKPDERII
KTEYGLLIRSLQKKDSGMYYCKAQEHTFIHTIVKLTLNVIENEQMENTQRAEHEEGKVKDLLAESRLRYKDYIQILSSPNFSLDQYCEQM

--------------------------------------------------------------

>23264_23264_6_DMTF1-SEMA3D_DMTF1_chr7_86800405_ENST00000394703_SEMA3D_chr7_84702397_ENST00000444867_length(amino acids)=280AA_BP=110
MSMSTVEEDSDTVTVETVNSVTLTQDTEGNLILHCPQNEADEIDSEDSIEPPHKRLCLSSEDDQSIDDSTPCISVVALPLSENDQSFEVT
MTATTEVADDEVTEGTVTQIQTECANFIRVLQPYNKTHIYVCGTGAFHPICGYIDLGVYKEDIIFKLDTHNLESGRLKCPFDPQQPFASV
MTDEYLYSGTASDFLGKDTAFTRSLGPTHDHHYIRTDISEHYWLNGAKFIGTFFIPDTYNPDDDKIYFFFRESSQEGSTSDKTILSRVGR

--------------------------------------------------------------

>23264_23264_7_DMTF1-SEMA3D_DMTF1_chr7_86800405_ENST00000411766_SEMA3D_chr7_84702397_ENST00000284136_length(amino acids)=722AA_BP=69
MSMSTVEEDSDTVTVETVNSVTLTQDTEGNLILHCPQNVSENDQSFEVTMTATTEVADDEVTEGTVTQIQTECANFIRVLQPYNKTHIYV
CGTGAFHPICGYIDLGVYKEDIIFKLDTHNLESGRLKCPFDPQQPFASVMTDEYLYSGTASDFLGKDTAFTRSLGPTHDHHYIRTDISEH
YWLNGAKFIGTFFIPDTYNPDDDKIYFFFRESSQEGSTSDKTILSRVGRVCKNDVGGQRSLINKWTTFLKARLICSIPGSDGADTYFDEL
QDIYLLPTRDERNPVVYGVFTTTSSIFKGSAVCVYSMADIRAVFNGPYAHKESADHRWVQYDGRIPYPRPGTCPSKTYDPLIKSTRDFPD
DVISFIKRHSVMYKSVYPVAGGPTFKRINVDYRLTQIVVDHVIAEDGQYDVMFLGTDIGTVLKVVSISKEKWNMEEVVLEELQIFKHSSI
ILNMELSLKQQQLYIGSRDGLVQLSLHRCDTYGKACADCCLARDPYCAWDGNACSRYAPTSKRRARRQDVKYGDPITQCWDIEDSISHET
ADEKVIFGIEFNSTFLECIPKSQQATIKWYIQRSGDEHREELKPDERIIKTEYGLLIRSLQKKDSGMYYCKAQEHTFIHTIVKLTLNVIE
NEQMENTQRAEHEEGKVKDLLAESRLRYKDYIQILSSPNFSLDQYCEQMWHREKRRQRNKGGPKWKHMQEMKKKRNRRHHRDLDELPRAV

--------------------------------------------------------------

>23264_23264_8_DMTF1-SEMA3D_DMTF1_chr7_86800405_ENST00000411766_SEMA3D_chr7_84702397_ENST00000444867_length(amino acids)=239AA_BP=69
MSMSTVEEDSDTVTVETVNSVTLTQDTEGNLILHCPQNVSENDQSFEVTMTATTEVADDEVTEGTVTQIQTECANFIRVLQPYNKTHIYV
CGTGAFHPICGYIDLGVYKEDIIFKLDTHNLESGRLKCPFDPQQPFASVMTDEYLYSGTASDFLGKDTAFTRSLGPTHDHHYIRTDISEH

--------------------------------------------------------------

>23264_23264_9_DMTF1-SEMA3D_DMTF1_chr7_86800405_ENST00000413276_SEMA3D_chr7_84702397_ENST00000284136_length(amino acids)=763AA_BP=110
MSMSTVEEDSDTVTVETVNSVTLTQDTEGNLILHCPQNEADEIDSEDSIEPPHKRLCLSSEDDQSIDDSTPCISVVALPLSENDQSFEVT
MTATTEVADDEVTEGTVTQIQTECANFIRVLQPYNKTHIYVCGTGAFHPICGYIDLGVYKEDIIFKLDTHNLESGRLKCPFDPQQPFASV
MTDEYLYSGTASDFLGKDTAFTRSLGPTHDHHYIRTDISEHYWLNGAKFIGTFFIPDTYNPDDDKIYFFFRESSQEGSTSDKTILSRVGR
VCKNDVGGQRSLINKWTTFLKARLICSIPGSDGADTYFDELQDIYLLPTRDERNPVVYGVFTTTSSIFKGSAVCVYSMADIRAVFNGPYA
HKESADHRWVQYDGRIPYPRPGTCPSKTYDPLIKSTRDFPDDVISFIKRHSVMYKSVYPVAGGPTFKRINVDYRLTQIVVDHVIAEDGQY
DVMFLGTDIGTVLKVVSISKEKWNMEEVVLEELQIFKHSSIILNMELSLKQQQLYIGSRDGLVQLSLHRCDTYGKACADCCLARDPYCAW
DGNACSRYAPTSKRRARRQDVKYGDPITQCWDIEDSISHETADEKVIFGIEFNSTFLECIPKSQQATIKWYIQRSGDEHREELKPDERII
KTEYGLLIRSLQKKDSGMYYCKAQEHTFIHTIVKLTLNVIENEQMENTQRAEHEEGKVKDLLAESRLRYKDYIQILSSPNFSLDQYCEQM

--------------------------------------------------------------

>23264_23264_10_DMTF1-SEMA3D_DMTF1_chr7_86800405_ENST00000413276_SEMA3D_chr7_84702397_ENST00000444867_length(amino acids)=280AA_BP=110
MSMSTVEEDSDTVTVETVNSVTLTQDTEGNLILHCPQNEADEIDSEDSIEPPHKRLCLSSEDDQSIDDSTPCISVVALPLSENDQSFEVT
MTATTEVADDEVTEGTVTQIQTECANFIRVLQPYNKTHIYVCGTGAFHPICGYIDLGVYKEDIIFKLDTHNLESGRLKCPFDPQQPFASV
MTDEYLYSGTASDFLGKDTAFTRSLGPTHDHHYIRTDISEHYWLNGAKFIGTFFIPDTYNPDDDKIYFFFRESSQEGSTSDKTILSRVGR

--------------------------------------------------------------

>23264_23264_11_DMTF1-SEMA3D_DMTF1_chr7_86800405_ENST00000432937_SEMA3D_chr7_84702397_ENST00000284136_length(amino acids)=706AA_BP=53
MSSEDDQSIDDSTPCISVVALPLSENDQSFEVTMTATTEVADDEVTEGTVTQIQTECANFIRVLQPYNKTHIYVCGTGAFHPICGYIDLG
VYKEDIIFKLDTHNLESGRLKCPFDPQQPFASVMTDEYLYSGTASDFLGKDTAFTRSLGPTHDHHYIRTDISEHYWLNGAKFIGTFFIPD
TYNPDDDKIYFFFRESSQEGSTSDKTILSRVGRVCKNDVGGQRSLINKWTTFLKARLICSIPGSDGADTYFDELQDIYLLPTRDERNPVV
YGVFTTTSSIFKGSAVCVYSMADIRAVFNGPYAHKESADHRWVQYDGRIPYPRPGTCPSKTYDPLIKSTRDFPDDVISFIKRHSVMYKSV
YPVAGGPTFKRINVDYRLTQIVVDHVIAEDGQYDVMFLGTDIGTVLKVVSISKEKWNMEEVVLEELQIFKHSSIILNMELSLKQQQLYIG
SRDGLVQLSLHRCDTYGKACADCCLARDPYCAWDGNACSRYAPTSKRRARRQDVKYGDPITQCWDIEDSISHETADEKVIFGIEFNSTFL
ECIPKSQQATIKWYIQRSGDEHREELKPDERIIKTEYGLLIRSLQKKDSGMYYCKAQEHTFIHTIVKLTLNVIENEQMENTQRAEHEEGK

--------------------------------------------------------------

>23264_23264_12_DMTF1-SEMA3D_DMTF1_chr7_86800405_ENST00000432937_SEMA3D_chr7_84702397_ENST00000444867_length(amino acids)=223AA_BP=53
MSSEDDQSIDDSTPCISVVALPLSENDQSFEVTMTATTEVADDEVTEGTVTQIQTECANFIRVLQPYNKTHIYVCGTGAFHPICGYIDLG
VYKEDIIFKLDTHNLESGRLKCPFDPQQPFASVMTDEYLYSGTASDFLGKDTAFTRSLGPTHDHHYIRTDISEHYWLNGAKFIGTFFIPD

--------------------------------------------------------------

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Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr7:86800405/chr7:84702397)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
DMTF1 Q9Y222	.
FUNCTION: Transcriptional activator which activates the CDKN2A/ARF locus in response to Ras-Raf signaling, thereby promoting p53/TP53-dependent growth arrest (By similarity). Binds to the consensus sequence 5'-CCCG[GT]ATGT-3' (By similarity). Isoform 1 may cooperate with MYB to activate transcription of the ANPEP gene. Isoform 2 may antagonize transcriptional activation by isoform 1. {ECO:0000250, ECO:0000269\|PubMed:12917399}.	FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Tgene

SEMA3D

chr7:86800405

chr7:84702397

ENST00000284136

739_773

125.0

778.0

Compositional bias

Note=Arg/Lys-rich (basic)

Tgene

SEMA3D

chr7:86800405

chr7:84702397

ENST00000284136

533_585

125.0

778.0

Domain

Note=PSI

Tgene

SEMA3D

chr7:86800405

chr7:84702397

ENST00000284136

592_680

125.0

778.0

Domain

Note=Ig-like C2-type

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

DMTF1

chr7:86800405

chr7:84702397

ENST00000331242

306_329

109.0

761.0

DNA binding

H-T-H motif

Hgene

DMTF1

chr7:86800405

chr7:84702397

ENST00000394702

306_329

109.0

286.0

DNA binding

H-T-H motif

Hgene

DMTF1

chr7:86800405

chr7:84702397

ENST00000394703

306_329

109.0

761.0

DNA binding

H-T-H motif

Hgene

DMTF1

chr7:86800405

chr7:84702397

ENST00000411766

306_329

68.0

230.0

DNA binding

H-T-H motif

Hgene

DMTF1

chr7:86800405

chr7:84702397

ENST00000432937

306_329

21.0

673.0

DNA binding

H-T-H motif

Hgene

DMTF1

chr7:86800405

chr7:84702397

ENST00000331242

225_263

109.0

761.0

Domain

Myb-like 1

Hgene

DMTF1

chr7:86800405

chr7:84702397

ENST00000331242

268_333

109.0

761.0

Domain

HTH myb-type

Hgene

DMTF1

chr7:86800405

chr7:84702397

ENST00000331242

339_388

109.0

761.0

Domain

Myb-like 2

Hgene

DMTF1

chr7:86800405

chr7:84702397

ENST00000394702

225_263

109.0

286.0

Domain

Myb-like 1

Hgene

DMTF1

chr7:86800405

chr7:84702397

ENST00000394702

268_333

109.0

286.0

Domain

HTH myb-type

Hgene

DMTF1

chr7:86800405

chr7:84702397

ENST00000394702

339_388

109.0

286.0

Domain

Myb-like 2

Hgene

DMTF1

chr7:86800405

chr7:84702397

ENST00000394703

225_263

109.0

761.0

Domain

Myb-like 1

Hgene

DMTF1

chr7:86800405

chr7:84702397

ENST00000394703

268_333

109.0

761.0

Domain

HTH myb-type

Hgene

DMTF1

chr7:86800405

chr7:84702397

ENST00000394703

339_388

109.0

761.0

Domain

Myb-like 2

Hgene

DMTF1

chr7:86800405

chr7:84702397

ENST00000411766

225_263

68.0

230.0

Domain

Myb-like 1

Hgene

DMTF1

chr7:86800405

chr7:84702397

ENST00000411766

268_333

68.0

230.0

Domain

HTH myb-type

Hgene

DMTF1

chr7:86800405

chr7:84702397

ENST00000411766

339_388

68.0

230.0

Domain

Myb-like 2

Hgene

DMTF1

chr7:86800405

chr7:84702397

ENST00000432937

225_263

21.0

673.0

Domain

Myb-like 1

Hgene

DMTF1

chr7:86800405

chr7:84702397

ENST00000432937

268_333

21.0

673.0

Domain

HTH myb-type

Hgene

DMTF1

chr7:86800405

chr7:84702397

ENST00000432937

339_388

21.0

673.0

Domain

Myb-like 2

Hgene

DMTF1

chr7:86800405

chr7:84702397

ENST00000331242

459_760

109.0

761.0

Region

Required for transcriptional activation

Hgene

DMTF1

chr7:86800405

chr7:84702397

ENST00000331242

87_170

109.0

761.0

Region

Required for transcriptional activation

Hgene

DMTF1

chr7:86800405

chr7:84702397

ENST00000331242

87_458

109.0

761.0

Region

Required for DNA-binding

Hgene

DMTF1

chr7:86800405

chr7:84702397

ENST00000394702

459_760

109.0

286.0

Region

Required for transcriptional activation

Hgene

DMTF1

chr7:86800405

chr7:84702397

ENST00000394702

87_170

109.0

286.0

Region

Required for transcriptional activation

Hgene

DMTF1

chr7:86800405

chr7:84702397

ENST00000394702

87_458

109.0

286.0

Region

Required for DNA-binding

Hgene

DMTF1

chr7:86800405

chr7:84702397

ENST00000394703

459_760

109.0

761.0

Region

Required for transcriptional activation

Hgene

DMTF1

chr7:86800405

chr7:84702397

ENST00000394703

87_170

109.0

761.0

Region

Required for transcriptional activation

Hgene

DMTF1

chr7:86800405

chr7:84702397

ENST00000394703

87_458

109.0

761.0

Region

Required for DNA-binding

Hgene

DMTF1

chr7:86800405

chr7:84702397

ENST00000411766

459_760

68.0

230.0

Region

Required for transcriptional activation

Hgene

DMTF1

chr7:86800405

chr7:84702397

ENST00000411766

87_170

68.0

230.0

Region

Required for transcriptional activation

Hgene

DMTF1

chr7:86800405

chr7:84702397

ENST00000411766

87_458

68.0

230.0

Region

Required for DNA-binding

Hgene

DMTF1

chr7:86800405

chr7:84702397

ENST00000432937

459_760

21.0

673.0

Region

Required for transcriptional activation

Hgene

DMTF1

chr7:86800405

chr7:84702397

ENST00000432937

87_170

21.0

673.0

Region

Required for transcriptional activation

Hgene

DMTF1

chr7:86800405

chr7:84702397

ENST00000432937

87_458

21.0

673.0

Region

Required for DNA-binding

Tgene

SEMA3D

chr7:86800405

chr7:84702397

ENST00000284136

44_531

125.0

778.0

Domain

Sema

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Fusion Protein Structures

PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.

Fusion protein PDB link (fusion AA seq ID in FusionPDB)	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	AA seq	Len(AA seq)
PDB file >>>1538_DMTF1_86800405_SEMA3D_84702397_ranked_0.pdb	DMTF1	86800405	86800405	ENST00000444867	SEMA3D	chr7	84702397	-	MSMSTVEEDSDTVTVETVNSVTLTQDTEGNLILHCPQNEADEIDSEDSIEPPHKRLCLSSEDDQSIDDSTPCISVVALPLSENDQSFEVT MTATTEVADDEVTEGTVTQIQTECANFIRVLQPYNKTHIYVCGTGAFHPICGYIDLGVYKEDIIFKLDTHNLESGRLKCPFDPQQPFASV MTDEYLYSGTASDFLGKDTAFTRSLGPTHDHHYIRTDISEHYWLNGAKFIGTFFIPDTYNPDDDKIYFFFRESSQEGSTSDKTILSRVGR VCKNDVGGQRSLINKWTTFLKARLICSIPGSDGADTYFDELQDIYLLPTRDERNPVVYGVFTTTSSIFKGSAVCVYSMADIRAVFNGPYA HKESADHRWVQYDGRIPYPRPGTCPSKTYDPLIKSTRDFPDDVISFIKRHSVMYKSVYPVAGGPTFKRINVDYRLTQIVVDHVIAEDGQY DVMFLGTDIGTVLKVVSISKEKWNMEEVVLEELQIFKHSSIILNMELSLKQQQLYIGSRDGLVQLSLHRCDTYGKACADCCLARDPYCAW DGNACSRYAPTSKRRARRQDVKYGDPITQCWDIEDSISHETADEKVIFGIEFNSTFLECIPKSQQATIKWYIQRSGDEHREELKPDERII KTEYGLLIRSLQKKDSGMYYCKAQEHTFIHTIVKLTLNVIENEQMENTQRAEHEEGKVKDLLAESRLRYKDYIQILSSPNFSLDQYCEQM	763

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pLDDT score distribution

pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

DMTF1_pLDDT.png

SEMA3D_pLDDT.png

pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

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Ramachandran Plot of Fusion Protein Structure

Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.

Fusion AA seq ID in FusionPDB and their Ramachandran plots

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Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
DMTF1
SEMA3D

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner	Gene	Hbp	Tbp	ENST	Strand	BPexon	TotalExon	Protein feature loci	*BPloci	TotalLen	Interaction lost with
Hgene	DMTF1	chr7:86800405	chr7:84702397	ENST00000331242	+	5	18	176_760	109.0	761.0	CCND1%2C CCND2 and CCND3
Hgene	DMTF1	chr7:86800405	chr7:84702397	ENST00000394702	+	5	10	176_760	109.0	286.0	CCND1%2C CCND2 and CCND3
Hgene	DMTF1	chr7:86800405	chr7:84702397	ENST00000394703	+	7	20	176_760	109.0	761.0	CCND1%2C CCND2 and CCND3
Hgene	DMTF1	chr7:86800405	chr7:84702397	ENST00000411766	+	4	10	176_760	68.0	230.0	CCND1%2C CCND2 and CCND3
Hgene	DMTF1	chr7:86800405	chr7:84702397	ENST00000432937	+	4	17	176_760	21.0	673.0	CCND1%2C CCND2 and CCND3
Hgene	DMTF1	chr7:86800405	chr7:84702397	ENST00000331242	+	5	18	1_237	109.0	761.0	CCND2
Hgene	DMTF1	chr7:86800405	chr7:84702397	ENST00000394702	+	5	10	1_237	109.0	286.0	CCND2
Hgene	DMTF1	chr7:86800405	chr7:84702397	ENST00000394703	+	7	20	1_237	109.0	761.0	CCND2
Hgene	DMTF1	chr7:86800405	chr7:84702397	ENST00000411766	+	4	10	1_237	68.0	230.0	CCND2
Hgene	DMTF1	chr7:86800405	chr7:84702397	ENST00000432937	+	4	17	1_237	21.0	673.0	CCND2

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Related Drugs to DMTF1-SEMA3D

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

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Related Diseases to DMTF1-SEMA3D

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source