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	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein Structure
	pLDDT scores
	Ramachandran Plot of Fusion Protein Structure
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:DNMT1-OLFM2

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: DNMT1-OLFM2
	FusionPDB ID: 23699
	FusionGDB2.0 ID: 23699
		Hgene	Tgene
	Gene symbol	DNMT1	OLFM2
	Gene ID	1786	93145
	Gene name	DNA methyltransferase 1	olfactomedin 2
	Synonyms	ADCADN\|AIM\|CXXC9\|DNMT\|HSN1E\|MCMT\|m.HsaI	NOE2\|NOELIN2\|NOELIN2_V1\|OlfC
	Cytomap	19p13.2	19p13.2
	Type of gene	protein-coding	protein-coding
	Description	DNA (cytosine-5)-methyltransferase 1CXXC-type zinc finger protein 9DNA (cytosine-5-)-methyltransferase 1DNA MTase HsaIDNA methyltransferase HsaI	noelin-2neuronal olfactomedin related ER localized protein 2
	Modification date	20200313	20200313
	UniProtAcc	P26358	.
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000340748, ENST00000359526, ENST00000540357, ENST00000589538,	ENST00000590841, ENST00000264833,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	14 X 12 X 11=1848	9 X 5 X 6=270
	# samples	17	10
	** MAII score	log2(17/1848*10)=-3.44235810527836 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(10/270*10)=-1.43295940727611 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)	PubMed: DNMT1 [Title/Abstract] AND OLFM2 [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	DNMT1(10341880)-OLFM2(9971470), # samples:2 DNMT1(10246412)-OLFM2(9968537), # samples:2
Anticipated loss of major functional domain due to fusion event.	DNMT1-OLFM2 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. DNMT1-OLFM2 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. DNMT1-OLFM2 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. DNMT1-OLFM2 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	DNMT1	GO:0010216	maintenance of DNA methylation	18754681\|21745816
Tgene	OLFM2	GO:0009306	protein secretion	21228389

Fusion gene breakpoints across DNMT1 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across OLFM2 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	BRCA	TCGA-B6-A0WY-01A	DNMT1	chr19	10341880	-	OLFM2	chr19	9971470	-
ChimerDB4	ESCA	TCGA-L5-A43I-01A	DNMT1	chr19	10246412	-	OLFM2	chr19	9968537	-
ChimerDB4	ESCA	TCGA-L5-A43I	DNMT1	chr19	10246412	-	OLFM2	chr19	9968537	-

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000340748	DNMT1	chr19	10246412	-	ENST00000264833	OLFM2	chr19	9968537	-	6581	4961	44	6112	2022
ENST00000540357	DNMT1	chr19	10246412	-	ENST00000264833	OLFM2	chr19	9968537	-	6534	4914	180	6065	1961
ENST00000359526	DNMT1	chr19	10246412	-	ENST00000264833	OLFM2	chr19	9968537	-	6573	4953	180	6104	1974

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000340748	ENST00000264833	DNMT1	chr19	10246412	-	OLFM2	chr19	9968537	-	0.00172198	0.998278
ENST00000540357	ENST00000264833	DNMT1	chr19	10246412	-	OLFM2	chr19	9968537	-	0.001063752	0.99893624
ENST00000359526	ENST00000264833	DNMT1	chr19	10246412	-	OLFM2	chr19	9968537	-	0.001945372	0.9980546

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>23699_23699_1_DNMT1-OLFM2_DNMT1_chr19_10246412_ENST00000340748_OLFM2_chr19_9968537_ENST00000264833_length(amino acids)=2022AA_BP=1
MGMAGSVPSFCTGYRLSPFGTSPPPPRPDWGGRRRLRSSPLPIGFRAKSRGACAAAAASAEASEMPARTAPARVPTLAVPAISLPDDVRR
RLKDLERDSLTEKECVKEKLNLLHEFLQTEIKNQLCDLETKLRKEELSEEGYLAKVKSLLNKDLSLENGAHAYNREVNGRLENGNQARSE
ARRVGMADANSPPKPLSKPRTPRRSKSDGEAKPEPSPSPRITRKSTRQTTITSHFAKGPAKRKPQEESERAKSDESIKEEDKDQDEKRRR
VTSRERVARPLPAEEPERAKSGTRTEKEEERDEKEEKRLRSQTKEPTPKQKLKEEPDREARAGVQADEDEDGDEKDEKKHRSQPKDLAAK
RRPEEKEPEKVNPQISDEKDEDEKEEKRRKTTPKEPTEKKMARAKTVMNSKTHPPKCIQCGQYLDDPDLKYGQHPPDAVDEPQMLTNEKL
SIFDANESGFESYEALPQHKLTCFSVYCKHGHLCPIDTGLIEKNIELFFSGSAKPIYDDDPSLEGGVNGKNLGPINEWWITGFDGGEKAL
IGFSTSFAEYILMDPSPEYAPIFGLMQEKIYISKIVVEFLQSNSDSTYEDLINKIETTVPPSGLNLNRFTEDSLLRHAQFVVEQVESYDE
AGDSDEQPIFLTPCMRDLIKLAGVTLGQRRAQARRQTIRHSTREKDRGPTKATTTKLVYQIFDTFFAEQIEKDDREDKENAFKRRRCGVC
EVCQQPECGKCKACKDMVKFGGSGRSKQACQERRCPNMAMKEADDDEEVDDNIPEMPSPKKMHQGKKKKQNKNRISWVGEAVKTDGKKSY
YKKVCIDAETLEVGDCVSVIPDDSSKPLYLARVTALWEDSSNGQMFHAHWFCAGTDTVLGATSDPLELFLVDECEDMQLSYIHSKVKVIY
KAPSENWAMEGGMDPESLLEGDDGKTYFYQLWYDQDYARFESPPKTQPTEDNKFKFCVSCARLAEMRQKEIPRVLEQLEDLDSRVLYYSA
TKNGILYRVGDGVYLPPEAFTFNIKLSSPVKRPRKEPVDEDLYPEHYRKYSDYIKGSNLDAPEPYRIGRIKEIFCPKKSNGRPNETDIKI
RVNKFYRPENTHKSTPASYHADINLLYWSDEEAVVDFKAVQGRCTVEYGEDLPECVQVYSMGGPNRFYFLEAYNAKSKSFEDPPNHARSP
GNKGKGKGKGKGKPKSQACEPSEPEIEIKLPKLRTLDVFSGCGGLSEGFHQAGISDTLWAIEMWDPAAQAFRLNNPGSTVFTEDCNILLK
LVMAGETTNSRGQRLPQKGDVEMLCGGPPCQGFSGMNRFNSRTYSKFKNSLVVSFLSYCDYYRPRFFLLENVRNFVSFKRSMVLKLTLRC
LVRMGYQCTFGVLQAGQYGVAQTRRRAIILAAAPGEKLPLFPEPLHVFAPRACQLSVVVDDKKFVSNITRLSSGPFRTITVRDTMSDLPE
VRNGASALEISYNGEPQSWFQRQLRGAQYQPILRDHICKDMSALVAARMRHIPLAPGSDWRDLPNIEVRLSDGTMARKLRYTHHDRKNGR
SSSGALRGVCSCVEAGKACDPAARQFNTLIPWCLPHTGNRHNHWAGLYGRLEWDGFFSTTVTNPEPMGKQGRVLHPEQHRVVSVRECARS
QGFPDTYRLFGNILDKHRQVQNVSQSMEVLELRTYRDLQYVRGMETLMRSLDARLRAADGSLSAKSFQELKDRMTELLPLSSVLEQYKAD
TRTIVRLREEVRNLSGSLAAIQEEMGAYGYEDLQQRVMALEARLHACAQKLGCGKLTGVSNPITVRAMGSRFGSWMTDTMAPSADSRVWY
MDGYYKGRRVLEFRTLGDFIKGQNFIQHLLPQPWAGTGHVVYNGSLFYNKYQSNVVVKYHFRSRSVLVQRSLPGAGYNNTFPYSWGGFSD
MDFMVDESGLWAVYTTNQNAGNIVVSRLDPHTLEVMRSWDTGYPKRSAGEAFMICGVLYVTNSHLAGAKVYFAYFTNTSSYEYTDVPFHN

--------------------------------------------------------------

>23699_23699_2_DNMT1-OLFM2_DNMT1_chr19_10246412_ENST00000359526_OLFM2_chr19_9968537_ENST00000264833_length(amino acids)=1974AA_BP=0
MPARTAPARVPTLAVPAISLPDDVRRRLKDLERDSLTEKECVKEKLNLLHEFLQTEIKNQLCDLETKLRKEELSEEGYLAKVKSLLNKDL
SLENGAHAYNREVNGRLENGNQARSEARRVGMADANSPPKPLSKPRTPRRSKSDGEAKRSRDPPASASQVTGIRAEPSPSPRITRKSTRQ
TTITSHFAKGPAKRKPQEESERAKSDESIKEEDKDQDEKRRRVTSRERVARPLPAEEPERAKSGTRTEKEEERDEKEEKRLRSQTKEPTP
KQKLKEEPDREARAGVQADEDEDGDEKDEKKHRSQPKDLAAKRRPEEKEPEKVNPQISDEKDEDEKEEKRRKTTPKEPTEKKMARAKTVM
NSKTHPPKCIQCGQYLDDPDLKYGQHPPDAVDEPQMLTNEKLSIFDANESGFESYEALPQHKLTCFSVYCKHGHLCPIDTGLIEKNIELF
FSGSAKPIYDDDPSLEGGVNGKNLGPINEWWITGFDGGEKALIGFSTSFAEYILMDPSPEYAPIFGLMQEKIYISKIVVEFLQSNSDSTY
EDLINKIETTVPPSGLNLNRFTEDSLLRHAQFVVEQVESYDEAGDSDEQPIFLTPCMRDLIKLAGVTLGQRRAQARRQTIRHSTREKDRG
PTKATTTKLVYQIFDTFFAEQIEKDDREDKENAFKRRRCGVCEVCQQPECGKCKACKDMVKFGGSGRSKQACQERRCPNMAMKEADDDEE
VDDNIPEMPSPKKMHQGKKKKQNKNRISWVGEAVKTDGKKSYYKKVCIDAETLEVGDCVSVIPDDSSKPLYLARVTALWEDSSNGQMFHA
HWFCAGTDTVLGATSDPLELFLVDECEDMQLSYIHSKVKVIYKAPSENWAMEGGMDPESLLEGDDGKTYFYQLWYDQDYARFESPPKTQP
TEDNKFKFCVSCARLAEMRQKEIPRVLEQLEDLDSRVLYYSATKNGILYRVGDGVYLPPEAFTFNIKLSSPVKRPRKEPVDEDLYPEHYR
KYSDYIKGSNLDAPEPYRIGRIKEIFCPKKSNGRPNETDIKIRVNKFYRPENTHKSTPASYHADINLLYWSDEEAVVDFKAVQGRCTVEY
GEDLPECVQVYSMGGPNRFYFLEAYNAKSKSFEDPPNHARSPGNKGKGKGKGKGKPKSQACEPSEPEIEIKLPKLRTLDVFSGCGGLSEG
FHQAGISDTLWAIEMWDPAAQAFRLNNPGSTVFTEDCNILLKLVMAGETTNSRGQRLPQKGDVEMLCGGPPCQGFSGMNRFNSRTYSKFK
NSLVVSFLSYCDYYRPRFFLLENVRNFVSFKRSMVLKLTLRCLVRMGYQCTFGVLQAGQYGVAQTRRRAIILAAAPGEKLPLFPEPLHVF
APRACQLSVVVDDKKFVSNITRLSSGPFRTITVRDTMSDLPEVRNGASALEISYNGEPQSWFQRQLRGAQYQPILRDHICKDMSALVAAR
MRHIPLAPGSDWRDLPNIEVRLSDGTMARKLRYTHHDRKNGRSSSGALRGVCSCVEAGKACDPAARQFNTLIPWCLPHTGNRHNHWAGLY
GRLEWDGFFSTTVTNPEPMGKQGRVLHPEQHRVVSVRECARSQGFPDTYRLFGNILDKHRQVQNVSQSMEVLELRTYRDLQYVRGMETLM
RSLDARLRAADGSLSAKSFQELKDRMTELLPLSSVLEQYKADTRTIVRLREEVRNLSGSLAAIQEEMGAYGYEDLQQRVMALEARLHACA
QKLGCGKLTGVSNPITVRAMGSRFGSWMTDTMAPSADSRVWYMDGYYKGRRVLEFRTLGDFIKGQNFIQHLLPQPWAGTGHVVYNGSLFY
NKYQSNVVVKYHFRSRSVLVQRSLPGAGYNNTFPYSWGGFSDMDFMVDESGLWAVYTTNQNAGNIVVSRLDPHTLEVMRSWDTGYPKRSA

--------------------------------------------------------------

>23699_23699_3_DNMT1-OLFM2_DNMT1_chr19_10246412_ENST00000540357_OLFM2_chr19_9968537_ENST00000264833_length(amino acids)=1961AA_BP=0
MPARTAPARVPTLAVPAISLPDDVRRRLKDLERDSLTEKECVKEKLNLLHEFLQTEIKNQLCDLETKLRKEELSEEGYLAKVKSLLNKDL
SLENGAHAYNREVNGRLENGNQARSEARRVGMADANSPPKPLSKPRTPRRSKSDGEAKPEPSPSPRITRKSTRQTTITSHFAKGPAKRKP
QEESERAKSDESIKEEDKDQDEKRRRVTSRERVARPLPAEEPERAKSGTRTEKEEERDEKEEKRLRSQTKEPTPKQKLKEEPDREARAGV
QADEDEDGDEKDEKKHRSQPKDLAAKRRPEEKEPEKVNPQISDEKDEDEKEEKRRKTTPKEPTEKKMARAKTVMNSKTHPPKCIQCGQYL
DDPDLKYGQHPPDAVDEPQMLTNEKLSIFDANESGFESYEALPQHKLTCFSVYCKHGHLCPIDTGLIEKNIELFFSGSAKPIYDDDPSLE
GGVNGKNLGPINEWWITGFDGGEKALIGFSTSFAEYILMDPSPEYAPIFGLMQEKIYISKIVVEFLQSNSDSTYEDLINKIETTVPPSGL
NLNRFTEDSLLRHAQFVVEQVESYDEAGDSDEQPIFLTPCMRDLIKLAGVTLGQRRAQARRQTIRHSTREKDRGPTKATTTKLVYQIFDT
FFAEQIEKDDREDKENAFKRRRCGVCEVCQQPECGKCKACKDMVKFGGSGRSKQACQERRCPNMAMKEADDDEEVDDNIPEMPSPKKMHQ
GKKKKQNKNRISWVGEAVKTDGKKSYYKKVCIDAETLEVGDCVSVIPDDSSKPLYLARVTALWEDSSNGQMFHAHWFCAGTDTVLGATSD
PLELFLVDECEDMQLSYIHSKVKVIYKAPSENWAMEGGMDPESLLEGDDGKTYFYQLWYDQDYARFESPPKTQPTEDNKFKFCVSCARLA
EMRQKEIPRVLEQLEDLDSRVLYYSATKNGILYRVGDGVYLPPEAFTFNIKLSSPVKRPRKEPVDEDLYPEHYRKYSDYIKGSNLDAPEP
YRIGRIKEIFCPKKSNGRPNETDIKIRVNKFYRPENTHKSTPASYHADINLLYWSDEEAVVDFKAVQGRCTVEYGEDLPECVQVYSMGGP
NRFYFLEAYNAKSKSFEDPPNHARSPGNKGKGKGKGKGKPKSQACEPSEPEIEIKLPKLRTLDVFSGCGGLSEGFHQAGISDTLWAIEMW
DPAAQAFRLNNPGSTVFTEDCNILLKLVMAGETTNSRGQRLPQKGDVEMLCGGPPCQGFSGMNRFNSRTYSKFKNSLVVSFLSYCDYYRP
RFFLLENVRNFVSFKRSMVLKLTLRCLVRMGYQCTFGVLQAGQYGVAQTRRRAIILAAAPGEKLPLFPEPLHVFAPRACQLSVVVDDKKF
VSNITRLSSGPFRTITVRDTMSDLPEVRNGASALEISYNGEPQSWFQRQLRGAQYQPILRDHICKDMSALVAARMRHIPLAPGSDWRDLP
NIEVRLSDGTMARKLRYTHHDRKNGRSSSGALRGVCSCVEGGSSGKACDPAARQFNTLIPWCLPHTGNRHNHWAGLYGRLEWDGFFSTTV
TNPEPMGKQGRVLHPEQHRVVSVRECARSQGFPDTYRLFGNILDKHRQVQNVSQSMEVLELRTYRDLQYVRGMETLMRSLDARLRAADGS
LSAKSFQELKDRMTELLPLSSVLEQYKADTRTIVRLREEVRNLSGSLAAIQEEMGAYGYEDLQQRVMALEARLHACAQKLGCGKLTGVSN
PITVRAMGSRFGSWMTDTMAPSADSRVWYMDGYYKGRRVLEFRTLGDFIKGQNFIQHLLPQPWAGTGHVVYNGSLFYNKYQSNVVVKYHF
RSRSVLVQRSLPGAGYNNTFPYSWGGFSDMDFMVDESGLWAVYTTNQNAGNIVVSRLDPHTLEVMRSWDTGYPKRSAGEAFMICGVLYVT

--------------------------------------------------------------

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Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr19:10341880/chr19:9971470)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
DNMT1 P26358	.
FUNCTION: Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed:24623306). Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed:24623306). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed:24623306). Promotes tumor growth (PubMed:24623306). {ECO:0000269\|PubMed:16357870, ECO:0000269\|PubMed:18413740, ECO:0000269\|PubMed:18754681, ECO:0000269\|PubMed:24623306}.	FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

DNMT1

chr19:10246412

chr19:9968537

ENST00000340748

16_109

1575.0

1617.0

Domain

DMAP1-binding

Hgene

DNMT1

chr19:10246412

chr19:9968537

ENST00000340748

755_880

1575.0

1617.0

Domain

BAH 1

Hgene

DNMT1

chr19:10246412

chr19:9968537

ENST00000340748

972_1100

1575.0

1617.0

Domain

BAH 2

Hgene

DNMT1

chr19:10246412

chr19:9968537

ENST00000359526

16_109

1591.0

1633.0

Domain

DMAP1-binding

Hgene

DNMT1

chr19:10246412

chr19:9968537

ENST00000359526

755_880

1591.0

1633.0

Domain

BAH 1

Hgene

DNMT1

chr19:10246412

chr19:9968537

ENST00000359526

972_1100

1591.0

1633.0

Domain

BAH 2

Hgene

DNMT1

chr19:10246412

chr19:9968537

ENST00000340748

177_205

1575.0

1617.0

Motif

Nuclear localization signal

Hgene

DNMT1

chr19:10246412

chr19:9968537

ENST00000359526

177_205

1591.0

1633.0

Motif

Nuclear localization signal

Hgene

DNMT1

chr19:10246412

chr19:9968537

ENST00000340748

1109_1120

1575.0

1617.0

Region

Note=6 X 2 AA tandem repeats of K-G

Hgene

DNMT1

chr19:10246412

chr19:9968537

ENST00000340748

1150_1151

1575.0

1617.0

Region

S-adenosyl-L-methionine binding

Hgene

DNMT1

chr19:10246412

chr19:9968537

ENST00000340748

1168_1169

1575.0

1617.0

Region

S-adenosyl-L-methionine binding

Hgene

DNMT1

chr19:10246412

chr19:9968537

ENST00000340748

1190_1191

1575.0

1617.0

Region

S-adenosyl-L-methionine binding

Hgene

DNMT1

chr19:10246412

chr19:9968537

ENST00000340748

310_502

1575.0

1617.0

Region

Note=Homodimerization

Hgene

DNMT1

chr19:10246412

chr19:9968537

ENST00000340748

331_550

1575.0

1617.0

Region

DNA replication foci-targeting sequence

Hgene

DNMT1

chr19:10246412

chr19:9968537

ENST00000340748

651_697

1575.0

1617.0

Region

Note=Required for activity

Hgene

DNMT1

chr19:10246412

chr19:9968537

ENST00000340748

693_754

1575.0

1617.0

Region

Note=Autoinhibitory linker

Hgene

DNMT1

chr19:10246412

chr19:9968537

ENST00000359526

1109_1120

1591.0

1633.0

Region

Note=6 X 2 AA tandem repeats of K-G

Hgene

DNMT1

chr19:10246412

chr19:9968537

ENST00000359526

1150_1151

1591.0

1633.0

Region

S-adenosyl-L-methionine binding

Hgene

DNMT1

chr19:10246412

chr19:9968537

ENST00000359526

1168_1169

1591.0

1633.0

Region

S-adenosyl-L-methionine binding

Hgene

DNMT1

chr19:10246412

chr19:9968537

ENST00000359526

1190_1191

1591.0

1633.0

Region

S-adenosyl-L-methionine binding

Hgene

DNMT1

chr19:10246412

chr19:9968537

ENST00000359526

310_502

1591.0

1633.0

Region

Note=Homodimerization

Hgene

DNMT1

chr19:10246412

chr19:9968537

ENST00000359526

331_550

1591.0

1633.0

Region

DNA replication foci-targeting sequence

Hgene

DNMT1

chr19:10246412

chr19:9968537

ENST00000359526

651_697

1591.0

1633.0

Region

Note=Required for activity

Hgene

DNMT1

chr19:10246412

chr19:9968537

ENST00000359526

693_754

1591.0

1633.0

Region

Note=Autoinhibitory linker

Hgene

DNMT1

chr19:10246412

chr19:9968537

ENST00000340748

1109_1110

1575.0

1617.0

Repeat

Note=1

Hgene

DNMT1

chr19:10246412

chr19:9968537

ENST00000340748

1111_1112

1575.0

1617.0

Repeat

Note=2

Hgene

DNMT1

chr19:10246412

chr19:9968537

ENST00000340748

1113_1114

1575.0

1617.0

Repeat

Note=3

Hgene

DNMT1

chr19:10246412

chr19:9968537

ENST00000340748

1115_1116

1575.0

1617.0

Repeat

Note=4

Hgene

DNMT1

chr19:10246412

chr19:9968537

ENST00000340748

1117_1118

1575.0

1617.0

Repeat

Note=5

Hgene

DNMT1

chr19:10246412

chr19:9968537

ENST00000340748

1119_1120

1575.0

1617.0

Repeat

Note=6%3B approximate

Hgene

DNMT1

chr19:10246412

chr19:9968537

ENST00000359526

1109_1110

1591.0

1633.0

Repeat

Note=1

Hgene

DNMT1

chr19:10246412

chr19:9968537

ENST00000359526

1111_1112

1591.0

1633.0

Repeat

Note=2

Hgene

DNMT1

chr19:10246412

chr19:9968537

ENST00000359526

1113_1114

1591.0

1633.0

Repeat

Note=3

Hgene

DNMT1

chr19:10246412

chr19:9968537

ENST00000359526

1115_1116

1591.0

1633.0

Repeat

Note=4

Hgene

DNMT1

chr19:10246412

chr19:9968537

ENST00000359526

1117_1118

1591.0

1633.0

Repeat

Note=5

Hgene

DNMT1

chr19:10246412

chr19:9968537

ENST00000359526

1119_1120

1591.0

1633.0

Repeat

Note=6%3B approximate

Hgene

DNMT1

chr19:10246412

chr19:9968537

ENST00000340748

646_692

1575.0

1617.0

Zinc finger

CXXC-type

Hgene

DNMT1

chr19:10246412

chr19:9968537

ENST00000359526

646_692

1591.0

1633.0

Zinc finger

CXXC-type

Tgene

OLFM2

chr19:10246412

chr19:9968537

ENST00000264833

136_193

71.0

455.0

Coiled coil

Ontology_term=ECO:0000255

Tgene

OLFM2

chr19:10246412

chr19:9968537

ENST00000264833

194_446

71.0

455.0

Domain

Olfactomedin-like

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

DNMT1

chr19:10246412

chr19:9968537

ENST00000340748

1139_1599

1575.0

1617.0

Domain

SAM-dependent MTase C5-type

Hgene

DNMT1

chr19:10246412

chr19:9968537

ENST00000359526

1139_1599

1591.0

1633.0

Domain

SAM-dependent MTase C5-type

Hgene

DNMT1

chr19:10246412

chr19:9968537

ENST00000340748

1139_1616

1575.0

1617.0

Region

Note=Catalytic

Hgene

DNMT1

chr19:10246412

chr19:9968537

ENST00000359526

1139_1616

1591.0

1633.0

Region

Note=Catalytic

Tgene

OLFM2

chr19:10246412

chr19:9968537

ENST00000264833

58_85

71.0

455.0

Coiled coil

Ontology_term=ECO:0000255

Top

Fusion Protein Structures

PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.

Fusion protein PDB link (fusion AA seq ID in FusionPDB)

Hgene

Hchr

Hbp

Hstrand

Tgene

Tchr

Tbp

Tstrand

AA seq

Len(AA seq)

Top

pLDDT score distribution

pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

DNMT1_pLDDT.png

OLFM2_pLDDT.png

pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

Top

Ramachandran Plot of Fusion Protein Structure

Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.

Fusion AA seq ID in FusionPDB and their Ramachandran plots

Top

Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
DNMT1
OLFM2

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner	Gene	Hbp	Tbp	ENST	Strand	BPexon	TotalExon	Protein feature loci	*BPloci	TotalLen	Interaction lost with
Hgene	DNMT1	chr19:10246412	chr19:9968537	ENST00000340748	-	38	40	1121_1616	1575.0	1617.0	the PRC2/EED-EZH2 complex
Hgene	DNMT1	chr19:10246412	chr19:9968537	ENST00000359526	-	39	41	1121_1616	1591.0	1633.0	the PRC2/EED-EZH2 complex

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Related Drugs to DNMT1-OLFM2

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

Top

Related Diseases to DNMT1-OLFM2

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source