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Center for Computational Systems Medicine level2
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Fusion Gene Summary

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Fusion Gene Sample Information

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Fusion ORF Analysis

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Fusion Amino Acid Sequences

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Fusion Protein Functional Features

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Fusion Protein Structure

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pLDDT scores

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Ramachandran Plot of Fusion Protein Structure

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Fusion Protein-Protein Interaction

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Related drugs with this fusion protein

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Related disease with this fusion protein

Fusion Protein:DYNLRB1-CNBD2

Fusion Protein Summary

check button Fusion gene summary
Fusion partner gene informationFusion gene name: DYNLRB1-CNBD2
FusionPDB ID: 24667
FusionGDB2.0 ID: 24667
HgeneTgene
Gene symbol

DYNLRB1

CNBD2

Gene ID

83658

140894

Gene namedynein light chain roadblock-type 1cyclic nucleotide binding domain containing 2
SynonymsBITH|BLP|DNCL2A|DNLC2A|ROBLD1C20orf152|CNMPD1
Cytomap

20q11.22

20q11.23

Type of geneprotein-codingprotein-coding
Descriptiondynein light chain roadblock-type 1ROBL/LC7-like 1bithoraxoid-like proteindynein, cytoplasmic, light polypeptide 2Adynein-associated protein Km23roadblock domain-containing protein 1cyclic nucleotide-binding domain-containing protein 2cyclic nucleotide (cNMP) binding domain containing 1
Modification date2020032020200313
UniProtAcc

Q9NP97

Q96M20

Ensembl transtripts involved in fusion geneENST idsENST00000480759, ENST00000300469, 
ENST00000357156, ENST00000374846, 
ENST00000417166, 
ENST00000349339, 
ENST00000373973, ENST00000538900, 
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)* DoF score9 X 6 X 5=27013 X 10 X 11=1430
# samples 1317
** MAII scorelog2(13/270*10)=-1.05444778402238
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
log2(17/1430*10)=-3.07240849552805
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)

PubMed: DYNLRB1 [Title/Abstract] AND CNBD2 [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)DYNLRB1(33114148)-CNBD2(34596219), # samples:2
Anticipated loss of major functional domain due to fusion event.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID

check buttonFusion gene breakpoints across DYNLRB1 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check buttonFusion gene breakpoints across CNBD2 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure


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Fusion Gene Sample Information

check buttonFusion gene information from FusionGDB2.0.
check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4PRADTCGA-HC-8257-01ADYNLRB1chr20

33114148

+CNBD2chr20

34596219

+
ChimerDB4PRADTCGA-HC-8257DYNLRB1chr20

33114148

+CNBD2chr20

34596219

+


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Fusion ORF Analysis


check buttonFusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)
BP loci
(transcript)
Predicted start
(transcript)
Predicted stop
(transcript)
Seq length
(amino acids)
ENST00000357156DYNLRB1chr2033114148+ENST00000373973CNBD2chr2034596219+94212950889279
ENST00000357156DYNLRB1chr2033114148+ENST00000349339CNBD2chr2034596219+93012950877275
ENST00000357156DYNLRB1chr2033114148+ENST00000538900CNBD2chr2034596219+74112950430126
ENST00000417166DYNLRB1chr2033114148+ENST00000373973CNBD2chr2034596219+92511233872279
ENST00000417166DYNLRB1chr2033114148+ENST00000349339CNBD2chr2034596219+91311233860275
ENST00000417166DYNLRB1chr2033114148+ENST00000538900CNBD2chr2034596219+72411233413126
ENST00000300469DYNLRB1chr2033114148+ENST00000373973CNBD2chr2034596219+9079415854279
ENST00000300469DYNLRB1chr2033114148+ENST00000349339CNBD2chr2034596219+8959415842275
ENST00000300469DYNLRB1chr2033114148+ENST00000538900CNBD2chr2034596219+7069415395126
ENST00000374846DYNLRB1chr2033114148+ENST00000373973CNBD2chr2034596219+13114982151258347
ENST00000374846DYNLRB1chr2033114148+ENST00000349339CNBD2chr2034596219+12994982151246343
ENST00000374846DYNLRB1chr2033114148+ENST00000538900CNBD2chr2034596219+1110498215799194

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000357156ENST00000373973DYNLRB1chr2033114148+CNBD2chr2034596219+0.0053464510.9946536
ENST00000357156ENST00000349339DYNLRB1chr2033114148+CNBD2chr2034596219+0.0045177740.9954822
ENST00000357156ENST00000538900DYNLRB1chr2033114148+CNBD2chr2034596219+0.0247006080.9752994
ENST00000417166ENST00000373973DYNLRB1chr2033114148+CNBD2chr2034596219+0.0051317960.99486816
ENST00000417166ENST00000349339DYNLRB1chr2033114148+CNBD2chr2034596219+0.0042086080.9957913
ENST00000417166ENST00000538900DYNLRB1chr2033114148+CNBD2chr2034596219+0.02119860.9788014
ENST00000300469ENST00000373973DYNLRB1chr2033114148+CNBD2chr2034596219+0.0043714440.9956286
ENST00000300469ENST00000349339DYNLRB1chr2033114148+CNBD2chr2034596219+0.003543580.99645644
ENST00000300469ENST00000538900DYNLRB1chr2033114148+CNBD2chr2034596219+0.0168021950.98319775
ENST00000374846ENST00000373973DYNLRB1chr2033114148+CNBD2chr2034596219+0.0050569910.99494296
ENST00000374846ENST00000349339DYNLRB1chr2033114148+CNBD2chr2034596219+0.0046583330.99534166
ENST00000374846ENST00000538900DYNLRB1chr2033114148+CNBD2chr2034596219+0.0236294720.97637045

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Fusion Amino Acid Sequences


check button For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>24667_24667_1_DYNLRB1-CNBD2_DYNLRB1_chr20_33114148_ENST00000300469_CNBD2_chr20_34596219_ENST00000349339_length(amino acids)=275AA_BP=26
MAEVEETLKRLQSQKGVQGIIVVNTEEYSPMERFKEFQIKSYPLQDFSSLKLPHLKKAWGLQGTSFSRKIRTSGDTLPKMLGPKIQSRPA
QSIKCAMINIKPGELPKEAAVGAYVKVHTVEQGEILAFLPEGECDTRPLILMSLGNELIRIRKEIFYELIDNDDEMIKKLLKLNIAFPSD
EDMCQKFLQQNSWNIFRKDLLQLLVEPCQSQLFTPNRPKKREIYNPKSVVLDLCSINKTTKPRYPIFMAPQKYLPPLRIVQAIKAPRYKI

--------------------------------------------------------------

>24667_24667_2_DYNLRB1-CNBD2_DYNLRB1_chr20_33114148_ENST00000300469_CNBD2_chr20_34596219_ENST00000373973_length(amino acids)=279AA_BP=26
MAEVEETLKRLQSQKGVQGIIVVNTEEYSPMERFKEFQIKSYPLQDFSSLKLPHLKKAWGLQGTSFSRKIRTSGDTLPKMLGPKIQSRPA
QSIKCAMINIKPGELPKEAAVGAYVKVHTVEQGEILGLHQAFLPEGECDTRPLILMSLGNELIRIRKEIFYELIDNDDEMIKKLLKLNIA
FPSDEDMCQKFLQQNSWNIFRKDLLQLLVEPCQSQLFTPNRPKKREIYNPKSVVLDLCSINKTTKPRYPIFMAPQKYLPPLRIVQAIKAP

--------------------------------------------------------------

>24667_24667_3_DYNLRB1-CNBD2_DYNLRB1_chr20_33114148_ENST00000300469_CNBD2_chr20_34596219_ENST00000538900_length(amino acids)=126AA_BP=26
MAEVEETLKRLQSQKGVQGIIVVNTEEYSPMERFKEFQIKSYPLQDFSSLKLPHLKKAWGLQGTSFSRKIRTSGDTLPKMLGPKIQSRPA

--------------------------------------------------------------

>24667_24667_4_DYNLRB1-CNBD2_DYNLRB1_chr20_33114148_ENST00000357156_CNBD2_chr20_34596219_ENST00000349339_length(amino acids)=275AA_BP=26
MAEVEETLKRLQSQKGVQGIIVVNTEEYSPMERFKEFQIKSYPLQDFSSLKLPHLKKAWGLQGTSFSRKIRTSGDTLPKMLGPKIQSRPA
QSIKCAMINIKPGELPKEAAVGAYVKVHTVEQGEILAFLPEGECDTRPLILMSLGNELIRIRKEIFYELIDNDDEMIKKLLKLNIAFPSD
EDMCQKFLQQNSWNIFRKDLLQLLVEPCQSQLFTPNRPKKREIYNPKSVVLDLCSINKTTKPRYPIFMAPQKYLPPLRIVQAIKAPRYKI

--------------------------------------------------------------

>24667_24667_5_DYNLRB1-CNBD2_DYNLRB1_chr20_33114148_ENST00000357156_CNBD2_chr20_34596219_ENST00000373973_length(amino acids)=279AA_BP=26
MAEVEETLKRLQSQKGVQGIIVVNTEEYSPMERFKEFQIKSYPLQDFSSLKLPHLKKAWGLQGTSFSRKIRTSGDTLPKMLGPKIQSRPA
QSIKCAMINIKPGELPKEAAVGAYVKVHTVEQGEILGLHQAFLPEGECDTRPLILMSLGNELIRIRKEIFYELIDNDDEMIKKLLKLNIA
FPSDEDMCQKFLQQNSWNIFRKDLLQLLVEPCQSQLFTPNRPKKREIYNPKSVVLDLCSINKTTKPRYPIFMAPQKYLPPLRIVQAIKAP

--------------------------------------------------------------

>24667_24667_6_DYNLRB1-CNBD2_DYNLRB1_chr20_33114148_ENST00000357156_CNBD2_chr20_34596219_ENST00000538900_length(amino acids)=126AA_BP=26
MAEVEETLKRLQSQKGVQGIIVVNTEEYSPMERFKEFQIKSYPLQDFSSLKLPHLKKAWGLQGTSFSRKIRTSGDTLPKMLGPKIQSRPA

--------------------------------------------------------------

>24667_24667_7_DYNLRB1-CNBD2_DYNLRB1_chr20_33114148_ENST00000374846_CNBD2_chr20_34596219_ENST00000349339_length(amino acids)=343AA_BP=1
MPLDRKREPSLGQEEMMGEGWAAALQPRVLGPWCAMATLATFPGSSVCGRLCQALYVSCISSASSQLCEAEVEETLKRLQSQKGVQGIIV
VNTEEYSPMERFKEFQIKSYPLQDFSSLKLPHLKKAWGLQGTSFSRKIRTSGDTLPKMLGPKIQSRPAQSIKCAMINIKPGELPKEAAVG
AYVKVHTVEQGEILAFLPEGECDTRPLILMSLGNELIRIRKEIFYELIDNDDEMIKKLLKLNIAFPSDEDMCQKFLQQNSWNIFRKDLLQ

--------------------------------------------------------------

>24667_24667_8_DYNLRB1-CNBD2_DYNLRB1_chr20_33114148_ENST00000374846_CNBD2_chr20_34596219_ENST00000373973_length(amino acids)=347AA_BP=1
MPLDRKREPSLGQEEMMGEGWAAALQPRVLGPWCAMATLATFPGSSVCGRLCQALYVSCISSASSQLCEAEVEETLKRLQSQKGVQGIIV
VNTEEYSPMERFKEFQIKSYPLQDFSSLKLPHLKKAWGLQGTSFSRKIRTSGDTLPKMLGPKIQSRPAQSIKCAMINIKPGELPKEAAVG
AYVKVHTVEQGEILGLHQAFLPEGECDTRPLILMSLGNELIRIRKEIFYELIDNDDEMIKKLLKLNIAFPSDEDMCQKFLQQNSWNIFRK

--------------------------------------------------------------

>24667_24667_9_DYNLRB1-CNBD2_DYNLRB1_chr20_33114148_ENST00000374846_CNBD2_chr20_34596219_ENST00000538900_length(amino acids)=194AA_BP=1
MPLDRKREPSLGQEEMMGEGWAAALQPRVLGPWCAMATLATFPGSSVCGRLCQALYVSCISSASSQLCEAEVEETLKRLQSQKGVQGIIV
VNTEEYSPMERFKEFQIKSYPLQDFSSLKLPHLKKAWGLQGTSFSRKIRTSGDTLPKMLGPKIQSRPAQSIKCAMINIKPGELPKEAAVG

--------------------------------------------------------------

>24667_24667_10_DYNLRB1-CNBD2_DYNLRB1_chr20_33114148_ENST00000417166_CNBD2_chr20_34596219_ENST00000349339_length(amino acids)=275AA_BP=26
MAEVEETLKRLQSQKGVQGIIVVNTEEYSPMERFKEFQIKSYPLQDFSSLKLPHLKKAWGLQGTSFSRKIRTSGDTLPKMLGPKIQSRPA
QSIKCAMINIKPGELPKEAAVGAYVKVHTVEQGEILAFLPEGECDTRPLILMSLGNELIRIRKEIFYELIDNDDEMIKKLLKLNIAFPSD
EDMCQKFLQQNSWNIFRKDLLQLLVEPCQSQLFTPNRPKKREIYNPKSVVLDLCSINKTTKPRYPIFMAPQKYLPPLRIVQAIKAPRYKI

--------------------------------------------------------------

>24667_24667_11_DYNLRB1-CNBD2_DYNLRB1_chr20_33114148_ENST00000417166_CNBD2_chr20_34596219_ENST00000373973_length(amino acids)=279AA_BP=26
MAEVEETLKRLQSQKGVQGIIVVNTEEYSPMERFKEFQIKSYPLQDFSSLKLPHLKKAWGLQGTSFSRKIRTSGDTLPKMLGPKIQSRPA
QSIKCAMINIKPGELPKEAAVGAYVKVHTVEQGEILGLHQAFLPEGECDTRPLILMSLGNELIRIRKEIFYELIDNDDEMIKKLLKLNIA
FPSDEDMCQKFLQQNSWNIFRKDLLQLLVEPCQSQLFTPNRPKKREIYNPKSVVLDLCSINKTTKPRYPIFMAPQKYLPPLRIVQAIKAP

--------------------------------------------------------------

>24667_24667_12_DYNLRB1-CNBD2_DYNLRB1_chr20_33114148_ENST00000417166_CNBD2_chr20_34596219_ENST00000538900_length(amino acids)=126AA_BP=26
MAEVEETLKRLQSQKGVQGIIVVNTEEYSPMERFKEFQIKSYPLQDFSSLKLPHLKKAWGLQGTSFSRKIRTSGDTLPKMLGPKIQSRPA

--------------------------------------------------------------

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Fusion Protein Functional Features


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr20:33114148/chr20:34596219)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
DYNLRB1

Q9NP97

CNBD2

Q96M20

FUNCTION: Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.FUNCTION: Essential for male fertility. Plays an important role in spermatogenesis and regulates sperm motility by controlling the development of the flagellar bending of sperm. {ECO:0000250|UniProtKB:Q9D5U8}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page

* Minus value of BPloci means that the break pointn is located before the CDS.
- Retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

- Not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
TgeneCNBD2chr20:33114148chr20:34596219ENST00000349339712116_239323.3333333333333573.0Nucleotide bindingNote=cNMP
TgeneCNBD2chr20:33114148chr20:34596219ENST00000373973712116_239323.3333333333333577.0Nucleotide bindingNote=cNMP
TgeneCNBD2chr20:33114148chr20:34596219ENST00000538900711116_239323.3333333333333424.0Nucleotide bindingNote=cNMP


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Fusion Protein Structures

check button PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.
Fusion protein PDB link (fusion AA seq ID in FusionPDB)HgeneHchrHbpHstrandTgeneTchrTbpTstrandAA seqLen(AA seq)
PDB file >>>666_DYNLRB1_33114148_CNBD2_34596219_ranked_0.pdbDYNLRB13311414833114148ENST00000538900CNBD2chr2034596219+
MPLDRKREPSLGQEEMMGEGWAAALQPRVLGPWCAMATLATFPGSSVCGRLCQALYVSCISSASSQLCEAEVEETLKRLQSQKGVQGIIV
VNTEEYSPMERFKEFQIKSYPLQDFSSLKLPHLKKAWGLQGTSFSRKIRTSGDTLPKMLGPKIQSRPAQSIKCAMINIKPGELPKEAAVG
AYVKVHTVEQGEILGLHQAFLPEGECDTRPLILMSLGNELIRIRKEIFYELIDNDDEMIKKLLKLNIAFPSDEDMCQKFLQQNSWNIFRK
347


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pLDDT score distribution

check button pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.
DYNLRB1_pLDDT.png
all structure
all structure
CNBD2_pLDDT.png
all structure
all structure

check button pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.
all structure


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Ramachandran Plot of Fusion Protein Structure


check button Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.
Fusion AA seq ID in FusionPDB and their Ramachandran plots

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Fusion Protein-Protein Interaction


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)
GenePPI interactors


check button Protein-protein interactors based on sequence similarity (STRING)
GeneSTRING network
DYNLRB1
CNBD2


check button - Retained interactions in fusion protein (protein functional feature from UniProt).
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost interactions due to fusion (protein functional feature from UniProt).
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs to DYNLRB1-CNBD2


check button Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDrugSourcePMID

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Related Diseases to DYNLRB1-CNBD2


check button Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDiseaseSourcePMID

check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource