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level2

	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein Structure
	pLDDT scores
	Ramachandran Plot of Fusion Protein Structure
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:ECT2-SPATA16

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: ECT2-SPATA16
	FusionPDB ID: 24922
	FusionGDB2.0 ID: 24922
		Hgene	Tgene
	Gene symbol	ECT2	SPATA16
	Gene ID	1894	83893
	Gene name	epithelial cell transforming 2	spermatogenesis associated 16
	Synonyms	ARHGEF31	NYD-SP12\|SPGF6
	Cytomap	3q26.31	3q26.31
	Type of gene	protein-coding	protein-coding
	Description	protein ECT2epithelial cell-transforming sequence 2 oncogene	spermatogenesis-associated protein 16testicular tissue protein Li 185testis development protein NYD-SP12testis-specific Golgi protein
	Modification date	20200329	20200313
	UniProtAcc	Q008S8	.
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000232458, ENST00000392692, ENST00000417960, ENST00000427830, ENST00000441497, ENST00000540509,	ENST00000351008,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	7 X 12 X 6=504	9 X 6 X 7=378
	# samples	12	10
	** MAII score	log2(12/504*10)=-2.0703893278914 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(10/378*10)=-1.91838623444635 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)	PubMed: ECT2 [Title/Abstract] AND SPATA16 [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	ECT2(172520770)-SPATA16(172766884), # samples:2
Anticipated loss of major functional domain due to fusion event.	ECT2-SPATA16 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. ECT2-SPATA16 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. ECT2-SPATA16 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. ECT2-SPATA16 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	ECT2	GO:0032147	activation of protein kinase activity	15254234
Hgene	ECT2	GO:0032467	positive regulation of cytokinesis	15545273
Hgene	ECT2	GO:0043065	positive regulation of apoptotic process	21373644
Hgene	ECT2	GO:0043547	positive regulation of GTPase activity	17115030\|19617897\|20047078
Hgene	ECT2	GO:0045859	regulation of protein kinase activity	15254234
Hgene	ECT2	GO:0051260	protein homooligomerization	15545273
Hgene	ECT2	GO:0070301	cellular response to hydrogen peroxide	21373644
Hgene	ECT2	GO:0071277	cellular response to calcium ion	15254234
Hgene	ECT2	GO:0071479	cellular response to ionizing radiation	21373644
Hgene	ECT2	GO:0090630	activation of GTPase activity	19617897

Fusion gene breakpoints across ECT2 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across SPATA16 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	STAD	TCGA-VQ-AA6D-01A	ECT2	chr3	172520770	+	SPATA16	chr3	172766884	-
ChimerDB4	STAD	TCGA-VQ-AA6D	ECT2	chr3	172520770	+	SPATA16	chr3	172766884	-

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000232458	ECT2	chr3	172520770	+	ENST00000351008	SPATA16	chr3	172766884	-	3521	2211	198	3308	1036
ENST00000392692	ECT2	chr3	172520770	+	ENST00000351008	SPATA16	chr3	172766884	-	3592	2282	176	3379	1067
ENST00000427830	ECT2	chr3	172520770	+	ENST00000351008	SPATA16	chr3	172766884	-	3480	2170	157	3267	1036
ENST00000417960	ECT2	chr3	172520770	+	ENST00000351008	SPATA16	chr3	172766884	-	3797	2487	477	3584	1035
ENST00000441497	ECT2	chr3	172520770	+	ENST00000351008	SPATA16	chr3	172766884	-	3397	2087	74	3184	1036
ENST00000540509	ECT2	chr3	172520770	+	ENST00000351008	SPATA16	chr3	172766884	-	3416	2106	0	3203	1067

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000232458	ENST00000351008	ECT2	chr3	172520770	+	SPATA16	chr3	172766884	-	0.000447648	0.9995523
ENST00000392692	ENST00000351008	ECT2	chr3	172520770	+	SPATA16	chr3	172766884	-	0.000259094	0.9997409
ENST00000427830	ENST00000351008	ECT2	chr3	172520770	+	SPATA16	chr3	172766884	-	0.000420652	0.99957937
ENST00000417960	ENST00000351008	ECT2	chr3	172520770	+	SPATA16	chr3	172766884	-	0.000691166	0.9993088
ENST00000441497	ENST00000351008	ECT2	chr3	172520770	+	SPATA16	chr3	172766884	-	0.000367367	0.9996326
ENST00000540509	ENST00000351008	ECT2	chr3	172520770	+	SPATA16	chr3	172766884	-	0.000208982	0.9997911

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>24922_24922_1_ECT2-SPATA16_ECT2_chr3_172520770_ENST00000232458_SPATA16_chr3_172766884_ENST00000351008_length(amino acids)=1036AA_BP=669
MAENSVLTSTTGRTSLADSSIFDSKVTEISKENLLIGSTSYVEEEMPQIETRVILVQEAGKQEELIKALKDIKVGFVKMESVEEFEGLDS
PEFENVFVVTDFQDSVFNDLYKADCRVIGPPVVLNCSQKGEPLPFSCRPLYCTSMMNLVLCFTGFRKKEELVRLVTLVHHMGGVIRKDFN
SKVTHLVANCTQGEKFRVAVSLGTPIMKPEWIYKAWERRNEQDFYAAVDDFRNEFKVPPFQDCILSFLGFSDEEKTNMEEMTEMQGGKYL
PLGDERCTHLVVEENIVKDLPFEPSKKLYVVKQEWFWGSIQMDARAGETMYLYEKANTPELKKSVSMLSLNTPNSNRKRRRLKETLAQLS
RETDVSPFPPRKRPSAEHSLSIGSLLDISNTPESSINYGDTPKSCTKSSKSSTPVPSKQSARWQVAKELYQTESNYVNILATIIQLFQVP
LEEEGQRGGPILAPEEIKTIFGSIPDIFDVHTKIKDDLEDLIVNWDESKSIGDIFLKYSKDLVKTYPPFVNFFEMSKETIIKCEKQKPRF
HAFLKINQAKPECGRQSLVELLIRPVQRLPSVALLLNDLKKHTADENPDKSTLEKAIGSLKEVMTHINEDKRKTEAQKQIFDVVYEVDGC
PANLLSSHRSLVQRVETISLGEHPCDRGEQVTLFLFNDCLELCSKGAVLGEPFDAPAEDIASVASFIETKLVTCYLRMRKPDLALNHAHR
SIVLNPAYFRNHLRQATVFRCLERYSEAARSAMIADYMFWLGGGREESISKLIKLYWQAMIEEAITRAESFSVMYTPFATKIRADKIEKV
KDAFTKTHPAYAEYMYTDLQALHMLPQTVDWSSFPPQQYLLTLGFKNKDDGKFLEKISSRKLPIFTEHKTPFGLTREDTVRQMETMGKRI
LPILDFIRSTQLNGSFPASSGVMEKLQYASLLSQLQRVKEQSQVINQAMAELATIPYLQDISQQEAELLQSLMADAMDTLEGRRNNNERV

--------------------------------------------------------------

>24922_24922_2_ECT2-SPATA16_ECT2_chr3_172520770_ENST00000392692_SPATA16_chr3_172766884_ENST00000351008_length(amino acids)=1067AA_BP=700
MAENSVLTSTTGRTSLADSSIFDSKVTEISKENLLIGSTSYVEEEMPQIETRVILVQEAGKQEELIKALKTIKIMEVPVIKIKESCPGKS
DEKLIKSVINMDIKVGFVKMESVEEFEGLDSPEFENVFVVTDFQDSVFNDLYKADCRVIGPPVVLNCSQKGEPLPFSCRPLYCTSMMNLV
LCFTGFRKKEELVRLVTLVHHMGGVIRKDFNSKVTHLVANCTQGEKFRVAVSLGTPIMKPEWIYKAWERRNEQDFYAAVDDFRNEFKVPP
FQDCILSFLGFSDEEKTNMEEMTEMQGGKYLPLGDERCTHLVVEENIVKDLPFEPSKKLYVVKQEWFWGSIQMDARAGETMYLYEKANTP
ELKKSVSMLSLNTPNSNRKRRRLKETLAQLSRETDVSPFPPRKRPSAEHSLSIGSLLDISNTPESSINYGDTPKSCTKSSKSSTPVPSKQ
SARWQVAKELYQTESNYVNILATIIQLFQVPLEEEGQRGGPILAPEEIKTIFGSIPDIFDVHTKIKDDLEDLIVNWDESKSIGDIFLKYS
KDLVKTYPPFVNFFEMSKETIIKCEKQKPRFHAFLKINQAKPECGRQSLVELLIRPVQRLPSVALLLNDLKKHTADENPDKSTLEKAIGS
LKEVMTHINEDKRKTEAQKQIFDVVYEVDGCPANLLSSHRSLVQRVETISLGEHPCDRGEQVTLFLFNDCLELCSKGAVLGEPFDAPAED
IASVASFIETKLVTCYLRMRKPDLALNHAHRSIVLNPAYFRNHLRQATVFRCLERYSEAARSAMIADYMFWLGGGREESISKLIKLYWQA
MIEEAITRAESFSVMYTPFATKIRADKIEKVKDAFTKTHPAYAEYMYTDLQALHMLPQTVDWSSFPPQQYLLTLGFKNKDDGKFLEKISS
RKLPIFTEHKTPFGLTREDTVRQMETMGKRILPILDFIRSTQLNGSFPASSGVMEKLQYASLLSQLQRVKEQSQVINQAMAELATIPYLQ

--------------------------------------------------------------

>24922_24922_3_ECT2-SPATA16_ECT2_chr3_172520770_ENST00000417960_SPATA16_chr3_172766884_ENST00000351008_length(amino acids)=1035AA_BP=668
MAENSVLTSTTGRTSLADSSIFDSKVTEISKENLLIGSTSYVEEMPQIETRVILVQEAGKQEELIKALKDIKVGFVKMESVEEFEGLDSP
EFENVFVVTDFQDSVFNDLYKADCRVIGPPVVLNCSQKGEPLPFSCRPLYCTSMMNLVLCFTGFRKKEELVRLVTLVHHMGGVIRKDFNS
KVTHLVANCTQGEKFRVAVSLGTPIMKPEWIYKAWERRNEQDFYAAVDDFRNEFKVPPFQDCILSFLGFSDEEKTNMEEMTEMQGGKYLP
LGDERCTHLVVEENIVKDLPFEPSKKLYVVKQEWFWGSIQMDARAGETMYLYEKANTPELKKSVSMLSLNTPNSNRKRRRLKETLAQLSR
ETDVSPFPPRKRPSAEHSLSIGSLLDISNTPESSINYGDTPKSCTKSSKSSTPVPSKQSARWQVAKELYQTESNYVNILATIIQLFQVPL
EEEGQRGGPILAPEEIKTIFGSIPDIFDVHTKIKDDLEDLIVNWDESKSIGDIFLKYSKDLVKTYPPFVNFFEMSKETIIKCEKQKPRFH
AFLKINQAKPECGRQSLVELLIRPVQRLPSVALLLNDLKKHTADENPDKSTLEKAIGSLKEVMTHINEDKRKTEAQKQIFDVVYEVDGCP
ANLLSSHRSLVQRVETISLGEHPCDRGEQVTLFLFNDCLELCSKGAVLGEPFDAPAEDIASVASFIETKLVTCYLRMRKPDLALNHAHRS
IVLNPAYFRNHLRQATVFRCLERYSEAARSAMIADYMFWLGGGREESISKLIKLYWQAMIEEAITRAESFSVMYTPFATKIRADKIEKVK
DAFTKTHPAYAEYMYTDLQALHMLPQTVDWSSFPPQQYLLTLGFKNKDDGKFLEKISSRKLPIFTEHKTPFGLTREDTVRQMETMGKRIL
PILDFIRSTQLNGSFPASSGVMEKLQYASLLSQLQRVKEQSQVINQAMAELATIPYLQDISQQEAELLQSLMADAMDTLEGRRNNNERVW

--------------------------------------------------------------

>24922_24922_4_ECT2-SPATA16_ECT2_chr3_172520770_ENST00000427830_SPATA16_chr3_172766884_ENST00000351008_length(amino acids)=1036AA_BP=669
MAENSVLTSTTGRTSLADSSIFDSKVTEISKENLLIGSTSYVEEEMPQIETRVILVQEAGKQEELIKALKDIKVGFVKMESVEEFEGLDS
PEFENVFVVTDFQDSVFNDLYKADCRVIGPPVVLNCSQKGEPLPFSCRPLYCTSMMNLVLCFTGFRKKEELVRLVTLVHHMGGVIRKDFN
SKVTHLVANCTQGEKFRVAVSLGTPIMKPEWIYKAWERRNEQDFYAAVDDFRNEFKVPPFQDCILSFLGFSDEEKTNMEEMTEMQGGKYL
PLGDERCTHLVVEENIVKDLPFEPSKKLYVVKQEWFWGSIQMDARAGETMYLYEKANTPELKKSVSMLSLNTPNSNRKRRRLKETLAQLS
RETDVSPFPPRKRPSAEHSLSIGSLLDISNTPESSINYGDTPKSCTKSSKSSTPVPSKQSARWQVAKELYQTESNYVNILATIIQLFQVP
LEEEGQRGGPILAPEEIKTIFGSIPDIFDVHTKIKDDLEDLIVNWDESKSIGDIFLKYSKDLVKTYPPFVNFFEMSKETIIKCEKQKPRF
HAFLKINQAKPECGRQSLVELLIRPVQRLPSVALLLNDLKKHTADENPDKSTLEKAIGSLKEVMTHINEDKRKTEAQKQIFDVVYEVDGC
PANLLSSHRSLVQRVETISLGEHPCDRGEQVTLFLFNDCLELCSKGAVLGEPFDAPAEDIASVASFIETKLVTCYLRMRKPDLALNHAHR
SIVLNPAYFRNHLRQATVFRCLERYSEAARSAMIADYMFWLGGGREESISKLIKLYWQAMIEEAITRAESFSVMYTPFATKIRADKIEKV
KDAFTKTHPAYAEYMYTDLQALHMLPQTVDWSSFPPQQYLLTLGFKNKDDGKFLEKISSRKLPIFTEHKTPFGLTREDTVRQMETMGKRI
LPILDFIRSTQLNGSFPASSGVMEKLQYASLLSQLQRVKEQSQVINQAMAELATIPYLQDISQQEAELLQSLMADAMDTLEGRRNNNERV

--------------------------------------------------------------

>24922_24922_5_ECT2-SPATA16_ECT2_chr3_172520770_ENST00000441497_SPATA16_chr3_172766884_ENST00000351008_length(amino acids)=1036AA_BP=669
MAENSVLTSTTGRTSLADSSIFDSKVTEISKENLLIGSTSYVEEEMPQIETRVILVQEAGKQEELIKALKDIKVGFVKMESVEEFEGLDS
PEFENVFVVTDFQDSVFNDLYKADCRVIGPPVVLNCSQKGEPLPFSCRPLYCTSMMNLVLCFTGFRKKEELVRLVTLVHHMGGVIRKDFN
SKVTHLVANCTQGEKFRVAVSLGTPIMKPEWIYKAWERRNEQDFYAAVDDFRNEFKVPPFQDCILSFLGFSDEEKTNMEEMTEMQGGKYL
PLGDERCTHLVVEENIVKDLPFEPSKKLYVVKQEWFWGSIQMDARAGETMYLYEKANTPELKKSVSMLSLNTPNSNRKRRRLKETLAQLS
RETDVSPFPPRKRPSAEHSLSIGSLLDISNTPESSINYGDTPKSCTKSSKSSTPVPSKQSARWQVAKELYQTESNYVNILATIIQLFQVP
LEEEGQRGGPILAPEEIKTIFGSIPDIFDVHTKIKDDLEDLIVNWDESKSIGDIFLKYSKDLVKTYPPFVNFFEMSKETIIKCEKQKPRF
HAFLKINQAKPECGRQSLVELLIRPVQRLPSVALLLNDLKKHTADENPDKSTLEKAIGSLKEVMTHINEDKRKTEAQKQIFDVVYEVDGC
PANLLSSHRSLVQRVETISLGEHPCDRGEQVTLFLFNDCLELCSKGAVLGEPFDAPAEDIASVASFIETKLVTCYLRMRKPDLALNHAHR
SIVLNPAYFRNHLRQATVFRCLERYSEAARSAMIADYMFWLGGGREESISKLIKLYWQAMIEEAITRAESFSVMYTPFATKIRADKIEKV
KDAFTKTHPAYAEYMYTDLQALHMLPQTVDWSSFPPQQYLLTLGFKNKDDGKFLEKISSRKLPIFTEHKTPFGLTREDTVRQMETMGKRI
LPILDFIRSTQLNGSFPASSGVMEKLQYASLLSQLQRVKEQSQVINQAMAELATIPYLQDISQQEAELLQSLMADAMDTLEGRRNNNERV

--------------------------------------------------------------

>24922_24922_6_ECT2-SPATA16_ECT2_chr3_172520770_ENST00000540509_SPATA16_chr3_172766884_ENST00000351008_length(amino acids)=1067AA_BP=700
MAENSVLTSTTGRTSLADSSIFDSKVTEISKENLLIGSTSYVEEEMPQIETRVILVQEAGKQEELIKALKTIKIMEVPVIKIKESCPGKS
DEKLIKSVINMDIKVGFVKMESVEEFEGLDSPEFENVFVVTDFQDSVFNDLYKADCRVIGPPVVLNCSQKGEPLPFSCRPLYCTSMMNLV
LCFTGFRKKEELVRLVTLVHHMGGVIRKDFNSKVTHLVANCTQGEKFRVAVSLGTPIMKPEWIYKAWERRNEQDFYAAVDDFRNEFKVPP
FQDCILSFLGFSDEEKTNMEEMTEMQGGKYLPLGDERCTHLVVEENIVKDLPFEPSKKLYVVKQEWFWGSIQMDARAGETMYLYEKANTP
ELKKSVSMLSLNTPNSNRKRRRLKETLAQLSRETDVSPFPPRKRPSAEHSLSIGSLLDISNTPESSINYGDTPKSCTKSSKSSTPVPSKQ
SARWQVAKELYQTESNYVNILATIIQLFQVPLEEEGQRGGPILAPEEIKTIFGSIPDIFDVHTKIKDDLEDLIVNWDESKSIGDIFLKYS
KDLVKTYPPFVNFFEMSKETIIKCEKQKPRFHAFLKINQAKPECGRQSLVELLIRPVQRLPSVALLLNDLKKHTADENPDKSTLEKAIGS
LKEVMTHINEDKRKTEAQKQIFDVVYEVDGCPANLLSSHRSLVQRVETISLGEHPCDRGEQVTLFLFNDCLELCSKGAVLGEPFDAPAED
IASVASFIETKLVTCYLRMRKPDLALNHAHRSIVLNPAYFRNHLRQATVFRCLERYSEAARSAMIADYMFWLGGGREESISKLIKLYWQA
MIEEAITRAESFSVMYTPFATKIRADKIEKVKDAFTKTHPAYAEYMYTDLQALHMLPQTVDWSSFPPQQYLLTLGFKNKDDGKFLEKISS
RKLPIFTEHKTPFGLTREDTVRQMETMGKRILPILDFIRSTQLNGSFPASSGVMEKLQYASLLSQLQRVKEQSQVINQAMAELATIPYLQ

--------------------------------------------------------------

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Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr3:172520770/chr3:172766884)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
ECT2 Q008S8	.
FUNCTION: May act as a guanine nucleotide exchange factor (GEF). {ECO:0000250}.	FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

ECT2

chr3:172520770

chr3:172766884

ENST00000232458

171_260

671.0

884.0

Domain

BRCT 1

Hgene

ECT2

chr3:172520770

chr3:172766884

ENST00000232458

266_354

671.0

884.0

Domain

BRCT 2

Hgene

ECT2

chr3:172520770

chr3:172766884

ENST00000232458

452_641

671.0

884.0

Domain

Hgene

ECT2

chr3:172520770

chr3:172766884

ENST00000392692

171_260

702.0

915.0

Domain

BRCT 1

Hgene

ECT2

chr3:172520770

chr3:172766884

ENST00000392692

266_354

702.0

915.0

Domain

BRCT 2

Hgene

ECT2

chr3:172520770

chr3:172766884

ENST00000392692

452_641

702.0

915.0

Domain

Hgene

ECT2

chr3:172520770

chr3:172766884

ENST00000417960

171_260

670.0

883.0

Domain

BRCT 1

Hgene

ECT2

chr3:172520770

chr3:172766884

ENST00000417960

266_354

670.0

883.0

Domain

BRCT 2

Hgene

ECT2

chr3:172520770

chr3:172766884

ENST00000417960

452_641

670.0

883.0

Domain

Hgene

ECT2

chr3:172520770

chr3:172766884

ENST00000441497

171_260

671.0

884.0

Domain

BRCT 1

Hgene

ECT2

chr3:172520770

chr3:172766884

ENST00000441497

266_354

671.0

884.0

Domain

BRCT 2

Hgene

ECT2

chr3:172520770

chr3:172766884

ENST00000441497

452_641

671.0

884.0

Domain

Hgene

ECT2

chr3:172520770

chr3:172766884

ENST00000540509

171_260

702.0

915.0

Domain

BRCT 1

Hgene

ECT2

chr3:172520770

chr3:172766884

ENST00000540509

266_354

702.0

915.0

Domain

BRCT 2

Hgene

ECT2

chr3:172520770

chr3:172766884

ENST00000540509

452_641

702.0

915.0

Domain

Hgene

ECT2

chr3:172520770

chr3:172766884

ENST00000232458

378_382

671.0

884.0

Motif

Note=Nuclear localization signal

Hgene

ECT2

chr3:172520770

chr3:172766884

ENST00000232458

401_405

671.0

884.0

Motif

Note=Nuclear localization signal

Hgene

ECT2

chr3:172520770

chr3:172766884

ENST00000392692

378_382

702.0

915.0

Motif

Note=Nuclear localization signal

Hgene

ECT2

chr3:172520770

chr3:172766884

ENST00000392692

401_405

702.0

915.0

Motif

Note=Nuclear localization signal

Hgene

ECT2

chr3:172520770

chr3:172766884

ENST00000417960

378_382

670.0

883.0

Motif

Note=Nuclear localization signal

Hgene

ECT2

chr3:172520770

chr3:172766884

ENST00000417960

401_405

670.0

883.0

Motif

Note=Nuclear localization signal

Hgene

ECT2

chr3:172520770

chr3:172766884

ENST00000441497

378_382

671.0

884.0

Motif

Note=Nuclear localization signal

Hgene

ECT2

chr3:172520770

chr3:172766884

ENST00000441497

401_405

671.0

884.0

Motif

Note=Nuclear localization signal

Hgene

ECT2

chr3:172520770

chr3:172766884

ENST00000540509

378_382

702.0

915.0

Motif

Note=Nuclear localization signal

Hgene

ECT2

chr3:172520770

chr3:172766884

ENST00000540509

401_405

702.0

915.0

Motif

Note=Nuclear localization signal

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

ECT2

chr3:172520770

chr3:172766884

ENST00000232458

675_794

671.0

884.0

Domain

Note=PH

Hgene

ECT2

chr3:172520770

chr3:172766884

ENST00000392692

675_794

702.0

915.0

Domain

Note=PH

Hgene

ECT2

chr3:172520770

chr3:172766884

ENST00000417960

675_794

670.0

883.0

Domain

Note=PH

Hgene

ECT2

chr3:172520770

chr3:172766884

ENST00000441497

675_794

671.0

884.0

Domain

Note=PH

Hgene

ECT2

chr3:172520770

chr3:172766884

ENST00000540509

675_794

702.0

915.0

Domain

Note=PH

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Fusion Protein Structures

PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.

Fusion protein PDB link (fusion AA seq ID in FusionPDB)	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	AA seq	Len(AA seq)
PDB file >>>1842_ECT2_172520770_SPATA16_172766884_ranked_0.pdb	ECT2	172520770	172520770	ENST00000351008	SPATA16	chr3	172766884	-	MAENSVLTSTTGRTSLADSSIFDSKVTEISKENLLIGSTSYVEEEMPQIETRVILVQEAGKQEELIKALKTIKIMEVPVIKIKESCPGKS DEKLIKSVINMDIKVGFVKMESVEEFEGLDSPEFENVFVVTDFQDSVFNDLYKADCRVIGPPVVLNCSQKGEPLPFSCRPLYCTSMMNLV LCFTGFRKKEELVRLVTLVHHMGGVIRKDFNSKVTHLVANCTQGEKFRVAVSLGTPIMKPEWIYKAWERRNEQDFYAAVDDFRNEFKVPP FQDCILSFLGFSDEEKTNMEEMTEMQGGKYLPLGDERCTHLVVEENIVKDLPFEPSKKLYVVKQEWFWGSIQMDARAGETMYLYEKANTP ELKKSVSMLSLNTPNSNRKRRRLKETLAQLSRETDVSPFPPRKRPSAEHSLSIGSLLDISNTPESSINYGDTPKSCTKSSKSSTPVPSKQ SARWQVAKELYQTESNYVNILATIIQLFQVPLEEEGQRGGPILAPEEIKTIFGSIPDIFDVHTKIKDDLEDLIVNWDESKSIGDIFLKYS KDLVKTYPPFVNFFEMSKETIIKCEKQKPRFHAFLKINQAKPECGRQSLVELLIRPVQRLPSVALLLNDLKKHTADENPDKSTLEKAIGS LKEVMTHINEDKRKTEAQKQIFDVVYEVDGCPANLLSSHRSLVQRVETISLGEHPCDRGEQVTLFLFNDCLELCSKGAVLGEPFDAPAED IASVASFIETKLVTCYLRMRKPDLALNHAHRSIVLNPAYFRNHLRQATVFRCLERYSEAARSAMIADYMFWLGGGREESISKLIKLYWQA MIEEAITRAESFSVMYTPFATKIRADKIEKVKDAFTKTHPAYAEYMYTDLQALHMLPQTVDWSSFPPQQYLLTLGFKNKDDGKFLEKISS RKLPIFTEHKTPFGLTREDTVRQMETMGKRILPILDFIRSTQLNGSFPASSGVMEKLQYASLLSQLQRVKEQSQVINQAMAELATIPYLQ	1067

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pLDDT score distribution

pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

ECT2_pLDDT.png

SPATA16_pLDDT.png

pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

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Ramachandran Plot of Fusion Protein Structure

Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.

Fusion AA seq ID in FusionPDB and their Ramachandran plots

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Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
ECT2
SPATA16

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

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Related Drugs to ECT2-SPATA16

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

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Related Diseases to ECT2-SPATA16

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source