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	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:EEF1G-ENG

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: EEF1G-ENG
	FusionPDB ID: 25201
	FusionGDB2.0 ID: 25201
		Hgene	Tgene
	Gene symbol	EEF1G	ENG
	Gene ID	1937	2022
	Gene name	eukaryotic translation elongation factor 1 gamma	endoglin
	Synonyms	EF1G\|GIG35	END\|HHT1\|ORW1
	Cytomap	11q12.3	9q34.11
	Type of gene	protein-coding	protein-coding
	Description	elongation factor 1-gammaEF-1-gammaPRO1608eEF-1B gammapancreatic tumor-related proteintranslation elongation factor eEF-1 gamma chain	endoglinCD105 antigen
	Modification date	20200313	20200329
	UniProtAcc	P26641	Q8NFI3
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000329251, ENST00000378019, ENST00000532986,	ENST00000480266, ENST00000344849, ENST00000373203,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	13 X 13 X 9=1521	6 X 7 X 4=168
	# samples	21	6
	** MAII score	log2(21/1521*10)=-2.85655892005837 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(6/168*10)=-1.48542682717024 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)	PubMed: EEF1G [Title/Abstract] AND ENG [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	EEF1G(62334870)-ENG(130605524), # samples:1
Anticipated loss of major functional domain due to fusion event.	EEF1G-ENG seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. EEF1G-ENG seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. EEF1G-ENG seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. EEF1G-ENG seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Tgene	ENG	GO:0001934	positive regulation of protein phosphorylation	12015308
Tgene	ENG	GO:0010862	positive regulation of pathway-restricted SMAD protein phosphorylation	12015308
Tgene	ENG	GO:0017015	regulation of transforming growth factor beta receptor signaling pathway	15702480
Tgene	ENG	GO:0030336	negative regulation of cell migration	19736306
Tgene	ENG	GO:0030513	positive regulation of BMP signaling pathway	17068149
Tgene	ENG	GO:0031953	negative regulation of protein autophosphorylation	12015308

Fusion gene breakpoints across EEF1G (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across ENG (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	UCEC	TCGA-EO-A1Y5	EEF1G	chr11	62334870	-	ENG	chr9	130605524	-

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000329251	EEF1G	chr11	62334870	-	ENST00000373203	ENG	chr9	130605524	-	3363	783	5	2692	895
ENST00000329251	EEF1G	chr11	62334870	-	ENST00000344849	ENG	chr9	130605524	-	3494	783	5	2593	862
ENST00000378019	EEF1G	chr11	62334870	-	ENST00000373203	ENG	chr9	130605524	-	3473	893	91	2802	903
ENST00000378019	EEF1G	chr11	62334870	-	ENST00000344849	ENG	chr9	130605524	-	3604	893	91	2703	870

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000329251	ENST00000373203	EEF1G	chr11	62334870	-	ENG	chr9	130605524	-	0.010438094	0.98956186
ENST00000329251	ENST00000344849	EEF1G	chr11	62334870	-	ENG	chr9	130605524	-	0.010686318	0.98931366
ENST00000378019	ENST00000373203	EEF1G	chr11	62334870	-	ENG	chr9	130605524	-	0.005064794	0.9949352
ENST00000378019	ENST00000344849	EEF1G	chr11	62334870	-	ENG	chr9	130605524	-	0.005283155	0.9947168

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>25201_25201_1_EEF1G-ENG_EEF1G_chr11_62334870_ENST00000329251_ENG_chr9_130605524_ENST00000344849_length(amino acids)=862AA_BP=259
MFRPRFPAAVSPRLSSFPLPSLPGGLLCGSAENPTPFLCGITMAAGTLYTYPENWRAFKALIAAQYSGAQVRVLSAPPHFHFGQTNRTPE
FLRKFPAGKVPAFEGDDGFCVFESNAIAYYVSNEELRGSTPEAAAQVVQWVSFADSDIVPPASTWVFPTLGIMHHNKQATENAKEEVRRI
LGLLDAYLKTRTFLVGERVTLADITVVCTLLWLYKQVLEPSFRQAFPNTNRWFLTCINQPQFRAVLGEVKLCEKMAQFDGLAETVHCDLQ
PVGPERGEVTYTTSQVSKGCVAQAPNAILEVHVLFLEFPTGPSQLELTLQASKQNGTWPREVLLVLSVNSSVFLHLQALGIPLHLAYNSS
LVTFQEPPGVNTTELPSFPKTQILEWAAERGPITSAAELNDPQSILLRLGQAQGSLSFCMLEASQDMGRTLEWRPRTPALVRGCHLEGVA
GHKEAHILRVLPGHSAGPRTVTVKVELSCAPGDLDAVLILQGPPYVSWLIDANHNMQIWTTGEYSFKIFPEKNIRGFKLPDTPQGLLGEA
RMLNASIVASFVELPLASIVSLHASSCGGRLQTSPAPIQTTPPKDTCSPELLMSLIQTKCADDAMTLVLKKELVAHLKCTITGLTFWDPS
CEAEDRGDKFVLRSAYSSCGMQVSASMISNEAVVNILSSSSPQRKKVHCLNMDSLSFQLGLYLSPHFLQASNTIEPGQQSFVQVRVSPSV
SEFLLQLDSCHLDLGPEGGTVELIQGRAAKGNCVSLLSPSPEGDPRFSFLLHFYTVPIPKTGTLSCTVALRPKTGSQDQEVHRTVFMRLN

--------------------------------------------------------------

>25201_25201_2_EEF1G-ENG_EEF1G_chr11_62334870_ENST00000329251_ENG_chr9_130605524_ENST00000373203_length(amino acids)=895AA_BP=259
MFRPRFPAAVSPRLSSFPLPSLPGGLLCGSAENPTPFLCGITMAAGTLYTYPENWRAFKALIAAQYSGAQVRVLSAPPHFHFGQTNRTPE
FLRKFPAGKVPAFEGDDGFCVFESNAIAYYVSNEELRGSTPEAAAQVVQWVSFADSDIVPPASTWVFPTLGIMHHNKQATENAKEEVRRI
LGLLDAYLKTRTFLVGERVTLADITVVCTLLWLYKQVLEPSFRQAFPNTNRWFLTCINQPQFRAVLGEVKLCEKMAQFDGLAETVHCDLQ
PVGPERGEVTYTTSQVSKGCVAQAPNAILEVHVLFLEFPTGPSQLELTLQASKQNGTWPREVLLVLSVNSSVFLHLQALGIPLHLAYNSS
LVTFQEPPGVNTTELPSFPKTQILEWAAERGPITSAAELNDPQSILLRLGQAQGSLSFCMLEASQDMGRTLEWRPRTPALVRGCHLEGVA
GHKEAHILRVLPGHSAGPRTVTVKVELSCAPGDLDAVLILQGPPYVSWLIDANHNMQIWTTGEYSFKIFPEKNIRGFKLPDTPQGLLGEA
RMLNASIVASFVELPLASIVSLHASSCGGRLQTSPAPIQTTPPKDTCSPELLMSLIQTKCADDAMTLVLKKELVAHLKCTITGLTFWDPS
CEAEDRGDKFVLRSAYSSCGMQVSASMISNEAVVNILSSSSPQRKKVHCLNMDSLSFQLGLYLSPHFLQASNTIEPGQQSFVQVRVSPSV
SEFLLQLDSCHLDLGPEGGTVELIQGRAAKGNCVSLLSPSPEGDPRFSFLLHFYTVPIPKTGTLSCTVALRPKTGSQDQEVHRTVFMRLN

--------------------------------------------------------------

>25201_25201_3_EEF1G-ENG_EEF1G_chr11_62334870_ENST00000378019_ENG_chr9_130605524_ENST00000344849_length(amino acids)=870AA_BP=267
MAERWVAPAVLRRARFASTFFLSPQIYAHKDGDLRSAFFILSFKRGEFIPFLNWTLYTYPENWRAFKALIAAQYSGAQVRVLSAPPHFHF
GQTNRTPEFLRKFPAGKVPAFEGDDGFCVFESNAIAYYVSNEELRGSTPEAAAQVVQWVSFADSDIVPPASTWVFPTLGIMHHNKQATEN
AKEEVRRILGLLDAYLKTRTFLVGERVTLADITVVCTLLWLYKQVLEPSFRQAFPNTNRWFLTCINQPQFRAVLGEVKLCEKMAQFDGLA
ETVHCDLQPVGPERGEVTYTTSQVSKGCVAQAPNAILEVHVLFLEFPTGPSQLELTLQASKQNGTWPREVLLVLSVNSSVFLHLQALGIP
LHLAYNSSLVTFQEPPGVNTTELPSFPKTQILEWAAERGPITSAAELNDPQSILLRLGQAQGSLSFCMLEASQDMGRTLEWRPRTPALVR
GCHLEGVAGHKEAHILRVLPGHSAGPRTVTVKVELSCAPGDLDAVLILQGPPYVSWLIDANHNMQIWTTGEYSFKIFPEKNIRGFKLPDT
PQGLLGEARMLNASIVASFVELPLASIVSLHASSCGGRLQTSPAPIQTTPPKDTCSPELLMSLIQTKCADDAMTLVLKKELVAHLKCTIT
GLTFWDPSCEAEDRGDKFVLRSAYSSCGMQVSASMISNEAVVNILSSSSPQRKKVHCLNMDSLSFQLGLYLSPHFLQASNTIEPGQQSFV
QVRVSPSVSEFLLQLDSCHLDLGPEGGTVELIQGRAAKGNCVSLLSPSPEGDPRFSFLLHFYTVPIPKTGTLSCTVALRPKTGSQDQEVH

--------------------------------------------------------------

>25201_25201_4_EEF1G-ENG_EEF1G_chr11_62334870_ENST00000378019_ENG_chr9_130605524_ENST00000373203_length(amino acids)=903AA_BP=267
MAERWVAPAVLRRARFASTFFLSPQIYAHKDGDLRSAFFILSFKRGEFIPFLNWTLYTYPENWRAFKALIAAQYSGAQVRVLSAPPHFHF
GQTNRTPEFLRKFPAGKVPAFEGDDGFCVFESNAIAYYVSNEELRGSTPEAAAQVVQWVSFADSDIVPPASTWVFPTLGIMHHNKQATEN
AKEEVRRILGLLDAYLKTRTFLVGERVTLADITVVCTLLWLYKQVLEPSFRQAFPNTNRWFLTCINQPQFRAVLGEVKLCEKMAQFDGLA
ETVHCDLQPVGPERGEVTYTTSQVSKGCVAQAPNAILEVHVLFLEFPTGPSQLELTLQASKQNGTWPREVLLVLSVNSSVFLHLQALGIP
LHLAYNSSLVTFQEPPGVNTTELPSFPKTQILEWAAERGPITSAAELNDPQSILLRLGQAQGSLSFCMLEASQDMGRTLEWRPRTPALVR
GCHLEGVAGHKEAHILRVLPGHSAGPRTVTVKVELSCAPGDLDAVLILQGPPYVSWLIDANHNMQIWTTGEYSFKIFPEKNIRGFKLPDT
PQGLLGEARMLNASIVASFVELPLASIVSLHASSCGGRLQTSPAPIQTTPPKDTCSPELLMSLIQTKCADDAMTLVLKKELVAHLKCTIT
GLTFWDPSCEAEDRGDKFVLRSAYSSCGMQVSASMISNEAVVNILSSSSPQRKKVHCLNMDSLSFQLGLYLSPHFLQASNTIEPGQQSFV
QVRVSPSVSEFLLQLDSCHLDLGPEGGTVELIQGRAAKGNCVSLLSPSPEGDPRFSFLLHFYTVPIPKTGTLSCTVALRPKTGSQDQEVH
RTVFMRLNIISPDLSGCTSKGLVLPAVLGITFGAFLIGALLTAALWYIYSHTRSPSKREPVVAVAAPASSESSSTNHSIGSTQSTPCSTS

--------------------------------------------------------------

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Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr11:62334870/chr9:130605524)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
EEF1G P26641	ENG Q8NFI3
FUNCTION: Probably plays a role in anchoring the complex to other cellular components.	FUNCTION: Endoglycosidase that releases N-glycans from glycoproteins by cleaving the beta-1,4-glycosidic bond in the N,N'-diacetylchitobiose core. Involved in the processing of free oligosaccharides in the cytosol. {ECO:0000269\|PubMed:12114544}.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

EEF1G

chr11:62334870

chr9:130605524

ENST00000329251

2_87

217.33333333333334

438.0

Domain

Note=GST N-terminal

Hgene

EEF1G

chr11:62334870

chr9:130605524

ENST00000329251

88_216

217.33333333333334

438.0

Domain

Note=GST C-terminal

Tgene

ENG

chr11:62334870

chr9:130605524

ENST00000344849

336_576

22.333333333333332

626.0

Compositional bias

Note=Ser/Thr-rich

Tgene

ENG

chr11:62334870

chr9:130605524

ENST00000373203

336_576

22.333333333333332

659.0

Compositional bias

Note=Ser/Thr-rich

Tgene

ENG

chr11:62334870

chr9:130605524

ENST00000344849

363_533

22.333333333333332

626.0

Domain

Tgene

ENG

chr11:62334870

chr9:130605524

ENST00000373203

363_533

22.333333333333332

659.0

Domain

Tgene

ENG

chr11:62334870

chr9:130605524

ENST00000344849

399_401

22.333333333333332

626.0

Motif

Cell attachment site

Tgene

ENG

chr11:62334870

chr9:130605524

ENST00000373203

399_401

22.333333333333332

659.0

Motif

Cell attachment site

Tgene

ENG

chr11:62334870

chr9:130605524

ENST00000344849

200_330

22.333333333333332

626.0

Region

OR1%2C C-terminal part

Tgene

ENG

chr11:62334870

chr9:130605524

ENST00000344849

26_46

22.333333333333332

626.0

Region

OR1%2C N-terminal part

Tgene

ENG

chr11:62334870

chr9:130605524

ENST00000344849

47_199

22.333333333333332

626.0

Region

OR2

Tgene

ENG

chr11:62334870

chr9:130605524

ENST00000373203

200_330

22.333333333333332

659.0

Region

OR1%2C C-terminal part

Tgene

ENG

chr11:62334870

chr9:130605524

ENST00000373203

26_46

22.333333333333332

659.0

Region

OR1%2C N-terminal part

Tgene

ENG

chr11:62334870

chr9:130605524

ENST00000373203

47_199

22.333333333333332

659.0

Region

OR2

Tgene

ENG

chr11:62334870

chr9:130605524

ENST00000344849

26_586

22.333333333333332

626.0

Topological domain

Extracellular

Tgene

ENG

chr11:62334870

chr9:130605524

ENST00000344849

612_658

22.333333333333332

626.0

Topological domain

Cytoplasmic

Tgene

ENG

chr11:62334870

chr9:130605524

ENST00000373203

26_586

22.333333333333332

659.0

Topological domain

Extracellular

Tgene

ENG

chr11:62334870

chr9:130605524

ENST00000373203

612_658

22.333333333333332

659.0

Topological domain

Cytoplasmic

Tgene

ENG

chr11:62334870

chr9:130605524

ENST00000344849

587_611

22.333333333333332

626.0

Transmembrane

Helical

Tgene

ENG

chr11:62334870

chr9:130605524

ENST00000373203

587_611

22.333333333333332

659.0

Transmembrane

Helical

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

EEF1G

chr11:62334870

chr9:130605524

ENST00000329251

276_437

217.33333333333334

438.0

Domain

EF-1-gamma C-terminal

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Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
EEF1G
ENG

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

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Related Drugs to EEF1G-ENG

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

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Related Diseases to EEF1G-ENG

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source