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	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein Structure
	pLDDT scores
	Ramachandran Plot of Fusion Protein Structure
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:EFR3A-ZFAT

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: EFR3A-ZFAT
	FusionPDB ID: 25405
	FusionGDB2.0 ID: 25405
		Hgene	Tgene
	Gene symbol	EFR3A	ZFAT
	Gene ID	23167	57623
	Gene name	EFR3 homolog A	zinc finger and AT-hook domain containing
	Synonyms	-	AITD3\|ZFAT1\|ZNF406
	Cytomap	8q24.22	8q24.22
	Type of gene	protein-coding	protein-coding
	Description	protein EFR3 homolog A	zinc finger protein ZFATzinc finger gene in autoimmune thyroid diseasezinc finger protein 406zinc-finger gene in AITD susceptibility region
	Modification date	20200313	20200313
	UniProtAcc	Q14156	.
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000254624, ENST00000334503, ENST00000519656, ENST00000521940,	ENST00000517307, ENST00000377838, ENST00000429442, ENST00000520214, ENST00000520356, ENST00000520727, ENST00000523399,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	11 X 7 X 7=539	7 X 7 X 6=294
	# samples	11	7
	** MAII score	log2(11/539*10)=-2.29278174922785 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(7/294*10)=-2.0703893278914 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)	PubMed: EFR3A [Title/Abstract] AND ZFAT [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	EFR3A(132999949)-ZFAT(135577664), # samples:4
Anticipated loss of major functional domain due to fusion event.	EFR3A-ZFAT seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. EFR3A-ZFAT seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. EFR3A-ZFAT seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. EFR3A-ZFAT seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner

Gene

GO ID

GO term

PubMed ID

Fusion gene breakpoints across EFR3A (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across ZFAT (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	LUAD	TCGA-78-8640-01A	EFR3A	chr8	132999949	-	ZFAT	chr8	135577664	-
ChimerDB4	LUAD	TCGA-78-8640-01A	EFR3A	chr8	132999949	+	ZFAT	chr8	135577664	-
ChimerDB4	LUAD	TCGA-78-8640	EFR3A	chr8	132999949	+	ZFAT	chr8	135577664	-

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000254624	EFR3A	chr8	132999949	+	ENST00000520356	ZFAT	chr8	135577664	-	3571	2290	186	2876	896
ENST00000254624	EFR3A	chr8	132999949	+	ENST00000520727	ZFAT	chr8	135577664	-	3825	2290	186	3134	982
ENST00000254624	EFR3A	chr8	132999949	+	ENST00000429442	ZFAT	chr8	135577664	-	3715	2290	186	2909	907
ENST00000254624	EFR3A	chr8	132999949	+	ENST00000377838	ZFAT	chr8	135577664	-	3825	2290	186	3134	982
ENST00000254624	EFR3A	chr8	132999949	+	ENST00000520214	ZFAT	chr8	135577664	-	3592	2290	186	3134	982
ENST00000254624	EFR3A	chr8	132999949	+	ENST00000523399	ZFAT	chr8	135577664	-	3224	2290	186	3134	982
ENST00000334503	EFR3A	chr8	132999949	+	ENST00000520356	ZFAT	chr8	135577664	-	3346	2065	0	2651	883
ENST00000334503	EFR3A	chr8	132999949	+	ENST00000520727	ZFAT	chr8	135577664	-	3600	2065	0	2909	969
ENST00000334503	EFR3A	chr8	132999949	+	ENST00000429442	ZFAT	chr8	135577664	-	3490	2065	0	2684	894
ENST00000334503	EFR3A	chr8	132999949	+	ENST00000377838	ZFAT	chr8	135577664	-	3600	2065	0	2909	969
ENST00000334503	EFR3A	chr8	132999949	+	ENST00000520214	ZFAT	chr8	135577664	-	3367	2065	0	2909	969
ENST00000334503	EFR3A	chr8	132999949	+	ENST00000523399	ZFAT	chr8	135577664	-	2999	2065	0	2909	969
ENST00000519656	EFR3A	chr8	132999949	+	ENST00000520356	ZFAT	chr8	135577664	-	3380	2099	67	2685	872
ENST00000519656	EFR3A	chr8	132999949	+	ENST00000520727	ZFAT	chr8	135577664	-	3634	2099	67	2943	958
ENST00000519656	EFR3A	chr8	132999949	+	ENST00000429442	ZFAT	chr8	135577664	-	3524	2099	67	2718	883
ENST00000519656	EFR3A	chr8	132999949	+	ENST00000377838	ZFAT	chr8	135577664	-	3634	2099	67	2943	958
ENST00000519656	EFR3A	chr8	132999949	+	ENST00000520214	ZFAT	chr8	135577664	-	3401	2099	67	2943	958
ENST00000519656	EFR3A	chr8	132999949	+	ENST00000523399	ZFAT	chr8	135577664	-	3033	2099	67	2943	958

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000254624	ENST00000520356	EFR3A	chr8	132999949	+	ZFAT	chr8	135577664	-	0.001269621	0.99873036
ENST00000254624	ENST00000520727	EFR3A	chr8	132999949	+	ZFAT	chr8	135577664	-	0.000951785	0.9990482
ENST00000254624	ENST00000429442	EFR3A	chr8	132999949	+	ZFAT	chr8	135577664	-	0.001456415	0.9985436
ENST00000254624	ENST00000377838	EFR3A	chr8	132999949	+	ZFAT	chr8	135577664	-	0.000951785	0.9990482
ENST00000254624	ENST00000520214	EFR3A	chr8	132999949	+	ZFAT	chr8	135577664	-	0.001509749	0.9984902
ENST00000254624	ENST00000523399	EFR3A	chr8	132999949	+	ZFAT	chr8	135577664	-	0.001672204	0.9983278
ENST00000334503	ENST00000520356	EFR3A	chr8	132999949	+	ZFAT	chr8	135577664	-	0.001291032	0.99870896
ENST00000334503	ENST00000520727	EFR3A	chr8	132999949	+	ZFAT	chr8	135577664	-	0.000969502	0.9990305
ENST00000334503	ENST00000429442	EFR3A	chr8	132999949	+	ZFAT	chr8	135577664	-	0.001415703	0.9985843
ENST00000334503	ENST00000377838	EFR3A	chr8	132999949	+	ZFAT	chr8	135577664	-	0.000969502	0.9990305
ENST00000334503	ENST00000520214	EFR3A	chr8	132999949	+	ZFAT	chr8	135577664	-	0.001515947	0.9984841
ENST00000334503	ENST00000523399	EFR3A	chr8	132999949	+	ZFAT	chr8	135577664	-	0.001781464	0.9982185
ENST00000519656	ENST00000520356	EFR3A	chr8	132999949	+	ZFAT	chr8	135577664	-	0.001350717	0.9986493
ENST00000519656	ENST00000520727	EFR3A	chr8	132999949	+	ZFAT	chr8	135577664	-	0.001223246	0.99877673
ENST00000519656	ENST00000429442	EFR3A	chr8	132999949	+	ZFAT	chr8	135577664	-	0.001669932	0.99833006
ENST00000519656	ENST00000377838	EFR3A	chr8	132999949	+	ZFAT	chr8	135577664	-	0.001223246	0.99877673
ENST00000519656	ENST00000520214	EFR3A	chr8	132999949	+	ZFAT	chr8	135577664	-	0.00191355	0.99808645
ENST00000519656	ENST00000523399	EFR3A	chr8	132999949	+	ZFAT	chr8	135577664	-	0.002160431	0.99783957

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>25405_25405_1_EFR3A-ZFAT_EFR3A_chr8_132999949_ENST00000254624_ZFAT_chr8_135577664_ENST00000377838_length(amino acids)=982AA_BP=701
MSLGAAASAVEIAMPTRVCCCCSALRPRYKRLVDNIFPEDPKDGLVKTDMEKLTFYAVSAPEKLDRIGSYLAERLSRDVVRHRSGYVLIA
MEALDQLLMACHSQSIKPFVESFLHMVAKLLESGEPKLQVLGTNSFVKFANIEEDTPSYHRRYDFFVSRFSAMCHSCHSDPEIRTEIRIA
GIRGIQGVVRKTVNDELRATIWEPQHMDKIVPSLLFNMQKIEEVDSRIGPPSSPSATDKEENPAVLAENCFRELLGRATFGNMNNAVRPV
FAHLDHHKLWDPNEFAVHCFKIIMYSIQAQYSHHVIQEILGHLDARKKDAPRVRAGIIQVLLEAVAIAAKGSIGPTVLEVFNTLLKHLRL
SVEFEANDLQGGSVGSVNLNTSSKDNDEKIVQNAIIQTIGFFGSNLPDYQRSEIMMFIMGKVPVFGTSTHTLDISQLGDLGTRRIQIMLL
RSLLMVTSGYKAKTIVTALPGSFLDPLLSPSLMEDYELRQLVLEVMHNLMDRHDNRAKLRGIRIIPDVADLKIKREKICRQDTSFMKKNG
QQLYRHIYLGCKEEDNVQKNYELLYTSLALITIELANEEVVIDLIRLAIALQDSAIINEDNLPMFHRCGIMALVAAYLNFVSQMIAVPAF
CQHVSKVIEIRTMEAPYFLPEHIFRDKCMLPKSLEKHEKDLYFLTNKIAESLGGSGYSVERLSVPYVPQVTGKQFKCTVCDYTAAQKPQL
LRHMEQHVSFKPFRCAHCHYSCNISGSLKRHYNRKHPNEEYANVGTGELAAEVLIQQGGLKCPVCSFVYGTKWEFNRHLKNKHGLKVVEI
DGDPKWETATEAPEEPSTQYLHITEAEEDVQGTQAAVAALQDLRYTSESGDRLDPTAVNILQQIIELGAETHDATALASVVAMAPGTVTV

--------------------------------------------------------------

>25405_25405_2_EFR3A-ZFAT_EFR3A_chr8_132999949_ENST00000254624_ZFAT_chr8_135577664_ENST00000429442_length(amino acids)=907AA_BP=701
MSLGAAASAVEIAMPTRVCCCCSALRPRYKRLVDNIFPEDPKDGLVKTDMEKLTFYAVSAPEKLDRIGSYLAERLSRDVVRHRSGYVLIA
MEALDQLLMACHSQSIKPFVESFLHMVAKLLESGEPKLQVLGTNSFVKFANIEEDTPSYHRRYDFFVSRFSAMCHSCHSDPEIRTEIRIA
GIRGIQGVVRKTVNDELRATIWEPQHMDKIVPSLLFNMQKIEEVDSRIGPPSSPSATDKEENPAVLAENCFRELLGRATFGNMNNAVRPV
FAHLDHHKLWDPNEFAVHCFKIIMYSIQAQYSHHVIQEILGHLDARKKDAPRVRAGIIQVLLEAVAIAAKGSIGPTVLEVFNTLLKHLRL
SVEFEANDLQGGSVGSVNLNTSSKDNDEKIVQNAIIQTIGFFGSNLPDYQRSEIMMFIMGKVPVFGTSTHTLDISQLGDLGTRRIQIMLL
RSLLMVTSGYKAKTIVTALPGSFLDPLLSPSLMEDYELRQLVLEVMHNLMDRHDNRAKLRGIRIIPDVADLKIKREKICRQDTSFMKKNG
QQLYRHIYLGCKEEDNVQKNYELLYTSLALITIELANEEVVIDLIRLAIALQDSAIINEDNLPMFHRCGIMALVAAYLNFVSQMIAVPAF
CQHVSKVIEIRTMEAPYFLPEHIFRDKCMLPKSLEKHEKDLYFLTNKIAESLGGSGYSVERLSVPYVPQVTGKQFKCTVCDYTAAQKPQL
LRHMEQHVSFKPFRCAHCHYSCNISGSLKRHYNRKHPNEEYANVGTGELAAEVLIQQGGLKCPVCSFVYGTKWEFNRHLKNKHGLKVVEI
DGDPKWETATEAPEEPSTQYLHITEAEEDVQGTQAAVAALQDLRYTSESGDRLDPTAVNILQQLQPHGHDPGDGPASVRGACRAAPPGGV

--------------------------------------------------------------

>25405_25405_3_EFR3A-ZFAT_EFR3A_chr8_132999949_ENST00000254624_ZFAT_chr8_135577664_ENST00000520214_length(amino acids)=982AA_BP=701
MSLGAAASAVEIAMPTRVCCCCSALRPRYKRLVDNIFPEDPKDGLVKTDMEKLTFYAVSAPEKLDRIGSYLAERLSRDVVRHRSGYVLIA
MEALDQLLMACHSQSIKPFVESFLHMVAKLLESGEPKLQVLGTNSFVKFANIEEDTPSYHRRYDFFVSRFSAMCHSCHSDPEIRTEIRIA
GIRGIQGVVRKTVNDELRATIWEPQHMDKIVPSLLFNMQKIEEVDSRIGPPSSPSATDKEENPAVLAENCFRELLGRATFGNMNNAVRPV
FAHLDHHKLWDPNEFAVHCFKIIMYSIQAQYSHHVIQEILGHLDARKKDAPRVRAGIIQVLLEAVAIAAKGSIGPTVLEVFNTLLKHLRL
SVEFEANDLQGGSVGSVNLNTSSKDNDEKIVQNAIIQTIGFFGSNLPDYQRSEIMMFIMGKVPVFGTSTHTLDISQLGDLGTRRIQIMLL
RSLLMVTSGYKAKTIVTALPGSFLDPLLSPSLMEDYELRQLVLEVMHNLMDRHDNRAKLRGIRIIPDVADLKIKREKICRQDTSFMKKNG
QQLYRHIYLGCKEEDNVQKNYELLYTSLALITIELANEEVVIDLIRLAIALQDSAIINEDNLPMFHRCGIMALVAAYLNFVSQMIAVPAF
CQHVSKVIEIRTMEAPYFLPEHIFRDKCMLPKSLEKHEKDLYFLTNKIAESLGGSGYSVERLSVPYVPQVTGKQFKCTVCDYTAAQKPQL
LRHMEQHVSFKPFRCAHCHYSCNISGSLKRHYNRKHPNEEYANVGTGELAAEVLIQQGGLKCPVCSFVYGTKWEFNRHLKNKHGLKVVEI
DGDPKWETATEAPEEPSTQYLHITEAEEDVQGTQAAVAALQDLRYTSESGDRLDPTAVNILQQIIELGAETHDATALASVVAMAPGTVTV

--------------------------------------------------------------

>25405_25405_4_EFR3A-ZFAT_EFR3A_chr8_132999949_ENST00000254624_ZFAT_chr8_135577664_ENST00000520356_length(amino acids)=896AA_BP=701
MSLGAAASAVEIAMPTRVCCCCSALRPRYKRLVDNIFPEDPKDGLVKTDMEKLTFYAVSAPEKLDRIGSYLAERLSRDVVRHRSGYVLIA
MEALDQLLMACHSQSIKPFVESFLHMVAKLLESGEPKLQVLGTNSFVKFANIEEDTPSYHRRYDFFVSRFSAMCHSCHSDPEIRTEIRIA
GIRGIQGVVRKTVNDELRATIWEPQHMDKIVPSLLFNMQKIEEVDSRIGPPSSPSATDKEENPAVLAENCFRELLGRATFGNMNNAVRPV
FAHLDHHKLWDPNEFAVHCFKIIMYSIQAQYSHHVIQEILGHLDARKKDAPRVRAGIIQVLLEAVAIAAKGSIGPTVLEVFNTLLKHLRL
SVEFEANDLQGGSVGSVNLNTSSKDNDEKIVQNAIIQTIGFFGSNLPDYQRSEIMMFIMGKVPVFGTSTHTLDISQLGDLGTRRIQIMLL
RSLLMVTSGYKAKTIVTALPGSFLDPLLSPSLMEDYELRQLVLEVMHNLMDRHDNRAKLRGIRIIPDVADLKIKREKICRQDTSFMKKNG
QQLYRHIYLGCKEEDNVQKNYELLYTSLALITIELANEEVVIDLIRLAIALQDSAIINEDNLPMFHRCGIMALVAAYLNFVSQMIAVPAF
CQHVSKVIEIRTMEAPYFLPEHIFRDKCMLPKSLEKHEKDLYFLTNKIAESLGGSGYSVERLSVPYVPQVTGKQFKCTVCDYTAAQKPQL
LRHMEQHVSFKPFRCAHCHYSCNISGSLKRHYNRKHPNEEYANVGTGELAAEVLIQQGGLKCPVCSFVYGTKWEFNRHLKNKHGLKVVEI

--------------------------------------------------------------

>25405_25405_5_EFR3A-ZFAT_EFR3A_chr8_132999949_ENST00000254624_ZFAT_chr8_135577664_ENST00000520727_length(amino acids)=982AA_BP=701
MSLGAAASAVEIAMPTRVCCCCSALRPRYKRLVDNIFPEDPKDGLVKTDMEKLTFYAVSAPEKLDRIGSYLAERLSRDVVRHRSGYVLIA
MEALDQLLMACHSQSIKPFVESFLHMVAKLLESGEPKLQVLGTNSFVKFANIEEDTPSYHRRYDFFVSRFSAMCHSCHSDPEIRTEIRIA
GIRGIQGVVRKTVNDELRATIWEPQHMDKIVPSLLFNMQKIEEVDSRIGPPSSPSATDKEENPAVLAENCFRELLGRATFGNMNNAVRPV
FAHLDHHKLWDPNEFAVHCFKIIMYSIQAQYSHHVIQEILGHLDARKKDAPRVRAGIIQVLLEAVAIAAKGSIGPTVLEVFNTLLKHLRL
SVEFEANDLQGGSVGSVNLNTSSKDNDEKIVQNAIIQTIGFFGSNLPDYQRSEIMMFIMGKVPVFGTSTHTLDISQLGDLGTRRIQIMLL
RSLLMVTSGYKAKTIVTALPGSFLDPLLSPSLMEDYELRQLVLEVMHNLMDRHDNRAKLRGIRIIPDVADLKIKREKICRQDTSFMKKNG
QQLYRHIYLGCKEEDNVQKNYELLYTSLALITIELANEEVVIDLIRLAIALQDSAIINEDNLPMFHRCGIMALVAAYLNFVSQMIAVPAF
CQHVSKVIEIRTMEAPYFLPEHIFRDKCMLPKSLEKHEKDLYFLTNKIAESLGGSGYSVERLSVPYVPQVTGKQFKCTVCDYTAAQKPQL
LRHMEQHVSFKPFRCAHCHYSCNISGSLKRHYNRKHPNEEYANVGTGELAAEVLIQQGGLKCPVCSFVYGTKWEFNRHLKNKHGLKVVEI
DGDPKWETATEAPEEPSTQYLHITEAEEDVQGTQAAVAALQDLRYTSESGDRLDPTAVNILQQIIELGAETHDATALASVVAMAPGTVTV

--------------------------------------------------------------

>25405_25405_6_EFR3A-ZFAT_EFR3A_chr8_132999949_ENST00000254624_ZFAT_chr8_135577664_ENST00000523399_length(amino acids)=982AA_BP=701
MSLGAAASAVEIAMPTRVCCCCSALRPRYKRLVDNIFPEDPKDGLVKTDMEKLTFYAVSAPEKLDRIGSYLAERLSRDVVRHRSGYVLIA
MEALDQLLMACHSQSIKPFVESFLHMVAKLLESGEPKLQVLGTNSFVKFANIEEDTPSYHRRYDFFVSRFSAMCHSCHSDPEIRTEIRIA
GIRGIQGVVRKTVNDELRATIWEPQHMDKIVPSLLFNMQKIEEVDSRIGPPSSPSATDKEENPAVLAENCFRELLGRATFGNMNNAVRPV
FAHLDHHKLWDPNEFAVHCFKIIMYSIQAQYSHHVIQEILGHLDARKKDAPRVRAGIIQVLLEAVAIAAKGSIGPTVLEVFNTLLKHLRL
SVEFEANDLQGGSVGSVNLNTSSKDNDEKIVQNAIIQTIGFFGSNLPDYQRSEIMMFIMGKVPVFGTSTHTLDISQLGDLGTRRIQIMLL
RSLLMVTSGYKAKTIVTALPGSFLDPLLSPSLMEDYELRQLVLEVMHNLMDRHDNRAKLRGIRIIPDVADLKIKREKICRQDTSFMKKNG
QQLYRHIYLGCKEEDNVQKNYELLYTSLALITIELANEEVVIDLIRLAIALQDSAIINEDNLPMFHRCGIMALVAAYLNFVSQMIAVPAF
CQHVSKVIEIRTMEAPYFLPEHIFRDKCMLPKSLEKHEKDLYFLTNKIAESLGGSGYSVERLSVPYVPQVTGKQFKCTVCDYTAAQKPQL
LRHMEQHVSFKPFRCAHCHYSCNISGSLKRHYNRKHPNEEYANVGTGELAAEVLIQQGGLKCPVCSFVYGTKWEFNRHLKNKHGLKVVEI
DGDPKWETATEAPEEPSTQYLHITEAEEDVQGTQAAVAALQDLRYTSESGDRLDPTAVNILQQIIELGAETHDATALASVVAMAPGTVTV

--------------------------------------------------------------

>25405_25405_7_EFR3A-ZFAT_EFR3A_chr8_132999949_ENST00000334503_ZFAT_chr8_135577664_ENST00000377838_length(amino acids)=969AA_BP=688
MKSRVCCCCSALRPRYKRLVDNIFPEDPKDGLVKTDMEKLTFYAVSAPEKLDRIGSYLAERLSRDVVRHRSGYVLIAMEALDQLLMACHS
QSIKPFVESFLHMVAKLLESGEPKLQVLGTNSFVKFANIEEDTPSYHRRYDFFVSRFSAMCHSCHSDPEIRTEIRIAGIRGIQGVVRKTV
NDELRATIWEPQHMDKIVPSLLFNMQKIEEVDSRIGPPSSPSATDKEENPAVLAENCFRELLGRATFGNMNNAVRPVFAHLDHHKLWDPN
EFAVHCFKIIMYSIQAQYSHHVIQEILGHLDARKKDAPRVRAGIIQVLLEAVAIAAKGSIGPTVLEVFNTLLKHLRLSVEFEANDLQGGS
VGSVNLNTSSKDNDEKIVQNAIIQTIGFFGSNLPDYQRSEIMMFIMGKVPVFGTSTHTLDISQLGDLGTRRIQIMLLRSLLMVTSGYKAK
TIVTALPGSFLDPLLSPSLMEDYELRQLVLEVMHNLMDRHDNRAKLRGIRIIPDVADLKIKREKICRQDTSFMKKNGQQLYRHIYLGCKE
EDNVQKNYELLYTSLALITIELANEEVVIDLIRLAIALQDSAIINEDNLPMFHRCGIMALVAAYLNFVSQMIAVPAFCQHVSKVIEIRTM
EAPYFLPEHIFRDKCMLPKSLEKHEKDLYFLTNKIAESLGGSGYSVERLSVPYVPQVTGKQFKCTVCDYTAAQKPQLLRHMEQHVSFKPF
RCAHCHYSCNISGSLKRHYNRKHPNEEYANVGTGELAAEVLIQQGGLKCPVCSFVYGTKWEFNRHLKNKHGLKVVEIDGDPKWETATEAP
EEPSTQYLHITEAEEDVQGTQAAVAALQDLRYTSESGDRLDPTAVNILQQIIELGAETHDATALASVVAMAPGTVTVVKQVTEEEPSSNH

--------------------------------------------------------------

>25405_25405_8_EFR3A-ZFAT_EFR3A_chr8_132999949_ENST00000334503_ZFAT_chr8_135577664_ENST00000429442_length(amino acids)=894AA_BP=688
MKSRVCCCCSALRPRYKRLVDNIFPEDPKDGLVKTDMEKLTFYAVSAPEKLDRIGSYLAERLSRDVVRHRSGYVLIAMEALDQLLMACHS
QSIKPFVESFLHMVAKLLESGEPKLQVLGTNSFVKFANIEEDTPSYHRRYDFFVSRFSAMCHSCHSDPEIRTEIRIAGIRGIQGVVRKTV
NDELRATIWEPQHMDKIVPSLLFNMQKIEEVDSRIGPPSSPSATDKEENPAVLAENCFRELLGRATFGNMNNAVRPVFAHLDHHKLWDPN
EFAVHCFKIIMYSIQAQYSHHVIQEILGHLDARKKDAPRVRAGIIQVLLEAVAIAAKGSIGPTVLEVFNTLLKHLRLSVEFEANDLQGGS
VGSVNLNTSSKDNDEKIVQNAIIQTIGFFGSNLPDYQRSEIMMFIMGKVPVFGTSTHTLDISQLGDLGTRRIQIMLLRSLLMVTSGYKAK
TIVTALPGSFLDPLLSPSLMEDYELRQLVLEVMHNLMDRHDNRAKLRGIRIIPDVADLKIKREKICRQDTSFMKKNGQQLYRHIYLGCKE
EDNVQKNYELLYTSLALITIELANEEVVIDLIRLAIALQDSAIINEDNLPMFHRCGIMALVAAYLNFVSQMIAVPAFCQHVSKVIEIRTM
EAPYFLPEHIFRDKCMLPKSLEKHEKDLYFLTNKIAESLGGSGYSVERLSVPYVPQVTGKQFKCTVCDYTAAQKPQLLRHMEQHVSFKPF
RCAHCHYSCNISGSLKRHYNRKHPNEEYANVGTGELAAEVLIQQGGLKCPVCSFVYGTKWEFNRHLKNKHGLKVVEIDGDPKWETATEAP

--------------------------------------------------------------

>25405_25405_9_EFR3A-ZFAT_EFR3A_chr8_132999949_ENST00000334503_ZFAT_chr8_135577664_ENST00000520214_length(amino acids)=969AA_BP=688
MKSRVCCCCSALRPRYKRLVDNIFPEDPKDGLVKTDMEKLTFYAVSAPEKLDRIGSYLAERLSRDVVRHRSGYVLIAMEALDQLLMACHS
QSIKPFVESFLHMVAKLLESGEPKLQVLGTNSFVKFANIEEDTPSYHRRYDFFVSRFSAMCHSCHSDPEIRTEIRIAGIRGIQGVVRKTV
NDELRATIWEPQHMDKIVPSLLFNMQKIEEVDSRIGPPSSPSATDKEENPAVLAENCFRELLGRATFGNMNNAVRPVFAHLDHHKLWDPN
EFAVHCFKIIMYSIQAQYSHHVIQEILGHLDARKKDAPRVRAGIIQVLLEAVAIAAKGSIGPTVLEVFNTLLKHLRLSVEFEANDLQGGS
VGSVNLNTSSKDNDEKIVQNAIIQTIGFFGSNLPDYQRSEIMMFIMGKVPVFGTSTHTLDISQLGDLGTRRIQIMLLRSLLMVTSGYKAK
TIVTALPGSFLDPLLSPSLMEDYELRQLVLEVMHNLMDRHDNRAKLRGIRIIPDVADLKIKREKICRQDTSFMKKNGQQLYRHIYLGCKE
EDNVQKNYELLYTSLALITIELANEEVVIDLIRLAIALQDSAIINEDNLPMFHRCGIMALVAAYLNFVSQMIAVPAFCQHVSKVIEIRTM
EAPYFLPEHIFRDKCMLPKSLEKHEKDLYFLTNKIAESLGGSGYSVERLSVPYVPQVTGKQFKCTVCDYTAAQKPQLLRHMEQHVSFKPF
RCAHCHYSCNISGSLKRHYNRKHPNEEYANVGTGELAAEVLIQQGGLKCPVCSFVYGTKWEFNRHLKNKHGLKVVEIDGDPKWETATEAP
EEPSTQYLHITEAEEDVQGTQAAVAALQDLRYTSESGDRLDPTAVNILQQIIELGAETHDATALASVVAMAPGTVTVVKQVTEEEPSSNH

--------------------------------------------------------------

>25405_25405_10_EFR3A-ZFAT_EFR3A_chr8_132999949_ENST00000334503_ZFAT_chr8_135577664_ENST00000520356_length(amino acids)=883AA_BP=688
MKSRVCCCCSALRPRYKRLVDNIFPEDPKDGLVKTDMEKLTFYAVSAPEKLDRIGSYLAERLSRDVVRHRSGYVLIAMEALDQLLMACHS
QSIKPFVESFLHMVAKLLESGEPKLQVLGTNSFVKFANIEEDTPSYHRRYDFFVSRFSAMCHSCHSDPEIRTEIRIAGIRGIQGVVRKTV
NDELRATIWEPQHMDKIVPSLLFNMQKIEEVDSRIGPPSSPSATDKEENPAVLAENCFRELLGRATFGNMNNAVRPVFAHLDHHKLWDPN
EFAVHCFKIIMYSIQAQYSHHVIQEILGHLDARKKDAPRVRAGIIQVLLEAVAIAAKGSIGPTVLEVFNTLLKHLRLSVEFEANDLQGGS
VGSVNLNTSSKDNDEKIVQNAIIQTIGFFGSNLPDYQRSEIMMFIMGKVPVFGTSTHTLDISQLGDLGTRRIQIMLLRSLLMVTSGYKAK
TIVTALPGSFLDPLLSPSLMEDYELRQLVLEVMHNLMDRHDNRAKLRGIRIIPDVADLKIKREKICRQDTSFMKKNGQQLYRHIYLGCKE
EDNVQKNYELLYTSLALITIELANEEVVIDLIRLAIALQDSAIINEDNLPMFHRCGIMALVAAYLNFVSQMIAVPAFCQHVSKVIEIRTM
EAPYFLPEHIFRDKCMLPKSLEKHEKDLYFLTNKIAESLGGSGYSVERLSVPYVPQVTGKQFKCTVCDYTAAQKPQLLRHMEQHVSFKPF
RCAHCHYSCNISGSLKRHYNRKHPNEEYANVGTGELAAEVLIQQGGLKCPVCSFVYGTKWEFNRHLKNKHGLKVVEIDGDPKWEVTEEEP

--------------------------------------------------------------

>25405_25405_11_EFR3A-ZFAT_EFR3A_chr8_132999949_ENST00000334503_ZFAT_chr8_135577664_ENST00000520727_length(amino acids)=969AA_BP=688
MKSRVCCCCSALRPRYKRLVDNIFPEDPKDGLVKTDMEKLTFYAVSAPEKLDRIGSYLAERLSRDVVRHRSGYVLIAMEALDQLLMACHS
QSIKPFVESFLHMVAKLLESGEPKLQVLGTNSFVKFANIEEDTPSYHRRYDFFVSRFSAMCHSCHSDPEIRTEIRIAGIRGIQGVVRKTV
NDELRATIWEPQHMDKIVPSLLFNMQKIEEVDSRIGPPSSPSATDKEENPAVLAENCFRELLGRATFGNMNNAVRPVFAHLDHHKLWDPN
EFAVHCFKIIMYSIQAQYSHHVIQEILGHLDARKKDAPRVRAGIIQVLLEAVAIAAKGSIGPTVLEVFNTLLKHLRLSVEFEANDLQGGS
VGSVNLNTSSKDNDEKIVQNAIIQTIGFFGSNLPDYQRSEIMMFIMGKVPVFGTSTHTLDISQLGDLGTRRIQIMLLRSLLMVTSGYKAK
TIVTALPGSFLDPLLSPSLMEDYELRQLVLEVMHNLMDRHDNRAKLRGIRIIPDVADLKIKREKICRQDTSFMKKNGQQLYRHIYLGCKE
EDNVQKNYELLYTSLALITIELANEEVVIDLIRLAIALQDSAIINEDNLPMFHRCGIMALVAAYLNFVSQMIAVPAFCQHVSKVIEIRTM
EAPYFLPEHIFRDKCMLPKSLEKHEKDLYFLTNKIAESLGGSGYSVERLSVPYVPQVTGKQFKCTVCDYTAAQKPQLLRHMEQHVSFKPF
RCAHCHYSCNISGSLKRHYNRKHPNEEYANVGTGELAAEVLIQQGGLKCPVCSFVYGTKWEFNRHLKNKHGLKVVEIDGDPKWETATEAP
EEPSTQYLHITEAEEDVQGTQAAVAALQDLRYTSESGDRLDPTAVNILQQIIELGAETHDATALASVVAMAPGTVTVVKQVTEEEPSSNH

--------------------------------------------------------------

>25405_25405_12_EFR3A-ZFAT_EFR3A_chr8_132999949_ENST00000334503_ZFAT_chr8_135577664_ENST00000523399_length(amino acids)=969AA_BP=688
MKSRVCCCCSALRPRYKRLVDNIFPEDPKDGLVKTDMEKLTFYAVSAPEKLDRIGSYLAERLSRDVVRHRSGYVLIAMEALDQLLMACHS
QSIKPFVESFLHMVAKLLESGEPKLQVLGTNSFVKFANIEEDTPSYHRRYDFFVSRFSAMCHSCHSDPEIRTEIRIAGIRGIQGVVRKTV
NDELRATIWEPQHMDKIVPSLLFNMQKIEEVDSRIGPPSSPSATDKEENPAVLAENCFRELLGRATFGNMNNAVRPVFAHLDHHKLWDPN
EFAVHCFKIIMYSIQAQYSHHVIQEILGHLDARKKDAPRVRAGIIQVLLEAVAIAAKGSIGPTVLEVFNTLLKHLRLSVEFEANDLQGGS
VGSVNLNTSSKDNDEKIVQNAIIQTIGFFGSNLPDYQRSEIMMFIMGKVPVFGTSTHTLDISQLGDLGTRRIQIMLLRSLLMVTSGYKAK
TIVTALPGSFLDPLLSPSLMEDYELRQLVLEVMHNLMDRHDNRAKLRGIRIIPDVADLKIKREKICRQDTSFMKKNGQQLYRHIYLGCKE
EDNVQKNYELLYTSLALITIELANEEVVIDLIRLAIALQDSAIINEDNLPMFHRCGIMALVAAYLNFVSQMIAVPAFCQHVSKVIEIRTM
EAPYFLPEHIFRDKCMLPKSLEKHEKDLYFLTNKIAESLGGSGYSVERLSVPYVPQVTGKQFKCTVCDYTAAQKPQLLRHMEQHVSFKPF
RCAHCHYSCNISGSLKRHYNRKHPNEEYANVGTGELAAEVLIQQGGLKCPVCSFVYGTKWEFNRHLKNKHGLKVVEIDGDPKWETATEAP
EEPSTQYLHITEAEEDVQGTQAAVAALQDLRYTSESGDRLDPTAVNILQQIIELGAETHDATALASVVAMAPGTVTVVKQVTEEEPSSNH

--------------------------------------------------------------

>25405_25405_13_EFR3A-ZFAT_EFR3A_chr8_132999949_ENST00000519656_ZFAT_chr8_135577664_ENST00000377838_length(amino acids)=958AA_BP=677
MRPRYKRLVDNIFPEDPKDGLVKTDMEKLTFYAVSAPEKLDRIGSYLAERLSRDVVRHRSGYVLIAMEALDQLLMACHSQSIKPFVESFL
HMVAKLLESGEPKLQVLGTNSFVKFANIEEDTPSYHRRYDFFVSRFSAMCHSCHSDPEIRTEIRIAGIRGIQGVVRKTVNDELRATIWEP
QHMDKIVPSLLFNMQKIEEVDSRIGPPSSPSATDKEENPAVLAENCFRELLGRATFGNMNNAVRPVFAHLDHHKLWDPNEFAVHCFKIIM
YSIQAQYSHHVIQEILGHLDARKKDAPRVRAGIIQVLLEAVAIAAKGSIGPTVLEVFNTLLKHLRLSVEFEANDLQGGSVGSVNLNTSSK
DNDEKIVQNAIIQTIGFFGSNLPDYQRSEIMMFIMGKVPVFGTSTHTLDISQLGDLGTRRIQIMLLRSLLMVTSGYKAKTIVTALPGSFL
DPLLSPSLMEDYELRQLVLEVMHNLMDRHDNRAKLRGIRIIPDVADLKIKREKICRQDTSFMKKNGQQLYRHIYLGCKEEDNVQKNYELL
YTSLALITIELANEEVVIDLIRLAIALQDSAIINEDNLPMFHRCGIMALVAAYLNFVSQMIAVPAFCQHVSKVIEIRTMEAPYFLPEHIF
RDKCMLPKSLEKHEKDLYFLTNKIAESLGGSGYSVERLSVPYVPQVTGKQFKCTVCDYTAAQKPQLLRHMEQHVSFKPFRCAHCHYSCNI
SGSLKRHYNRKHPNEEYANVGTGELAAEVLIQQGGLKCPVCSFVYGTKWEFNRHLKNKHGLKVVEIDGDPKWETATEAPEEPSTQYLHIT
EAEEDVQGTQAAVAALQDLRYTSESGDRLDPTAVNILQQIIELGAETHDATALASVVAMAPGTVTVVKQVTEEEPSSNHTVMIQETVQQA

--------------------------------------------------------------

>25405_25405_14_EFR3A-ZFAT_EFR3A_chr8_132999949_ENST00000519656_ZFAT_chr8_135577664_ENST00000429442_length(amino acids)=883AA_BP=677
MRPRYKRLVDNIFPEDPKDGLVKTDMEKLTFYAVSAPEKLDRIGSYLAERLSRDVVRHRSGYVLIAMEALDQLLMACHSQSIKPFVESFL
HMVAKLLESGEPKLQVLGTNSFVKFANIEEDTPSYHRRYDFFVSRFSAMCHSCHSDPEIRTEIRIAGIRGIQGVVRKTVNDELRATIWEP
QHMDKIVPSLLFNMQKIEEVDSRIGPPSSPSATDKEENPAVLAENCFRELLGRATFGNMNNAVRPVFAHLDHHKLWDPNEFAVHCFKIIM
YSIQAQYSHHVIQEILGHLDARKKDAPRVRAGIIQVLLEAVAIAAKGSIGPTVLEVFNTLLKHLRLSVEFEANDLQGGSVGSVNLNTSSK
DNDEKIVQNAIIQTIGFFGSNLPDYQRSEIMMFIMGKVPVFGTSTHTLDISQLGDLGTRRIQIMLLRSLLMVTSGYKAKTIVTALPGSFL
DPLLSPSLMEDYELRQLVLEVMHNLMDRHDNRAKLRGIRIIPDVADLKIKREKICRQDTSFMKKNGQQLYRHIYLGCKEEDNVQKNYELL
YTSLALITIELANEEVVIDLIRLAIALQDSAIINEDNLPMFHRCGIMALVAAYLNFVSQMIAVPAFCQHVSKVIEIRTMEAPYFLPEHIF
RDKCMLPKSLEKHEKDLYFLTNKIAESLGGSGYSVERLSVPYVPQVTGKQFKCTVCDYTAAQKPQLLRHMEQHVSFKPFRCAHCHYSCNI
SGSLKRHYNRKHPNEEYANVGTGELAAEVLIQQGGLKCPVCSFVYGTKWEFNRHLKNKHGLKVVEIDGDPKWETATEAPEEPSTQYLHIT

--------------------------------------------------------------

>25405_25405_15_EFR3A-ZFAT_EFR3A_chr8_132999949_ENST00000519656_ZFAT_chr8_135577664_ENST00000520214_length(amino acids)=958AA_BP=677
MRPRYKRLVDNIFPEDPKDGLVKTDMEKLTFYAVSAPEKLDRIGSYLAERLSRDVVRHRSGYVLIAMEALDQLLMACHSQSIKPFVESFL
HMVAKLLESGEPKLQVLGTNSFVKFANIEEDTPSYHRRYDFFVSRFSAMCHSCHSDPEIRTEIRIAGIRGIQGVVRKTVNDELRATIWEP
QHMDKIVPSLLFNMQKIEEVDSRIGPPSSPSATDKEENPAVLAENCFRELLGRATFGNMNNAVRPVFAHLDHHKLWDPNEFAVHCFKIIM
YSIQAQYSHHVIQEILGHLDARKKDAPRVRAGIIQVLLEAVAIAAKGSIGPTVLEVFNTLLKHLRLSVEFEANDLQGGSVGSVNLNTSSK
DNDEKIVQNAIIQTIGFFGSNLPDYQRSEIMMFIMGKVPVFGTSTHTLDISQLGDLGTRRIQIMLLRSLLMVTSGYKAKTIVTALPGSFL
DPLLSPSLMEDYELRQLVLEVMHNLMDRHDNRAKLRGIRIIPDVADLKIKREKICRQDTSFMKKNGQQLYRHIYLGCKEEDNVQKNYELL
YTSLALITIELANEEVVIDLIRLAIALQDSAIINEDNLPMFHRCGIMALVAAYLNFVSQMIAVPAFCQHVSKVIEIRTMEAPYFLPEHIF
RDKCMLPKSLEKHEKDLYFLTNKIAESLGGSGYSVERLSVPYVPQVTGKQFKCTVCDYTAAQKPQLLRHMEQHVSFKPFRCAHCHYSCNI
SGSLKRHYNRKHPNEEYANVGTGELAAEVLIQQGGLKCPVCSFVYGTKWEFNRHLKNKHGLKVVEIDGDPKWETATEAPEEPSTQYLHIT
EAEEDVQGTQAAVAALQDLRYTSESGDRLDPTAVNILQQIIELGAETHDATALASVVAMAPGTVTVVKQVTEEEPSSNHTVMIQETVQQA

--------------------------------------------------------------

>25405_25405_16_EFR3A-ZFAT_EFR3A_chr8_132999949_ENST00000519656_ZFAT_chr8_135577664_ENST00000520356_length(amino acids)=872AA_BP=677
MRPRYKRLVDNIFPEDPKDGLVKTDMEKLTFYAVSAPEKLDRIGSYLAERLSRDVVRHRSGYVLIAMEALDQLLMACHSQSIKPFVESFL
HMVAKLLESGEPKLQVLGTNSFVKFANIEEDTPSYHRRYDFFVSRFSAMCHSCHSDPEIRTEIRIAGIRGIQGVVRKTVNDELRATIWEP
QHMDKIVPSLLFNMQKIEEVDSRIGPPSSPSATDKEENPAVLAENCFRELLGRATFGNMNNAVRPVFAHLDHHKLWDPNEFAVHCFKIIM
YSIQAQYSHHVIQEILGHLDARKKDAPRVRAGIIQVLLEAVAIAAKGSIGPTVLEVFNTLLKHLRLSVEFEANDLQGGSVGSVNLNTSSK
DNDEKIVQNAIIQTIGFFGSNLPDYQRSEIMMFIMGKVPVFGTSTHTLDISQLGDLGTRRIQIMLLRSLLMVTSGYKAKTIVTALPGSFL
DPLLSPSLMEDYELRQLVLEVMHNLMDRHDNRAKLRGIRIIPDVADLKIKREKICRQDTSFMKKNGQQLYRHIYLGCKEEDNVQKNYELL
YTSLALITIELANEEVVIDLIRLAIALQDSAIINEDNLPMFHRCGIMALVAAYLNFVSQMIAVPAFCQHVSKVIEIRTMEAPYFLPEHIF
RDKCMLPKSLEKHEKDLYFLTNKIAESLGGSGYSVERLSVPYVPQVTGKQFKCTVCDYTAAQKPQLLRHMEQHVSFKPFRCAHCHYSCNI
SGSLKRHYNRKHPNEEYANVGTGELAAEVLIQQGGLKCPVCSFVYGTKWEFNRHLKNKHGLKVVEIDGDPKWEVTEEEPSSNHTVMIQET

--------------------------------------------------------------

>25405_25405_17_EFR3A-ZFAT_EFR3A_chr8_132999949_ENST00000519656_ZFAT_chr8_135577664_ENST00000520727_length(amino acids)=958AA_BP=677
MRPRYKRLVDNIFPEDPKDGLVKTDMEKLTFYAVSAPEKLDRIGSYLAERLSRDVVRHRSGYVLIAMEALDQLLMACHSQSIKPFVESFL
HMVAKLLESGEPKLQVLGTNSFVKFANIEEDTPSYHRRYDFFVSRFSAMCHSCHSDPEIRTEIRIAGIRGIQGVVRKTVNDELRATIWEP
QHMDKIVPSLLFNMQKIEEVDSRIGPPSSPSATDKEENPAVLAENCFRELLGRATFGNMNNAVRPVFAHLDHHKLWDPNEFAVHCFKIIM
YSIQAQYSHHVIQEILGHLDARKKDAPRVRAGIIQVLLEAVAIAAKGSIGPTVLEVFNTLLKHLRLSVEFEANDLQGGSVGSVNLNTSSK
DNDEKIVQNAIIQTIGFFGSNLPDYQRSEIMMFIMGKVPVFGTSTHTLDISQLGDLGTRRIQIMLLRSLLMVTSGYKAKTIVTALPGSFL
DPLLSPSLMEDYELRQLVLEVMHNLMDRHDNRAKLRGIRIIPDVADLKIKREKICRQDTSFMKKNGQQLYRHIYLGCKEEDNVQKNYELL
YTSLALITIELANEEVVIDLIRLAIALQDSAIINEDNLPMFHRCGIMALVAAYLNFVSQMIAVPAFCQHVSKVIEIRTMEAPYFLPEHIF
RDKCMLPKSLEKHEKDLYFLTNKIAESLGGSGYSVERLSVPYVPQVTGKQFKCTVCDYTAAQKPQLLRHMEQHVSFKPFRCAHCHYSCNI
SGSLKRHYNRKHPNEEYANVGTGELAAEVLIQQGGLKCPVCSFVYGTKWEFNRHLKNKHGLKVVEIDGDPKWETATEAPEEPSTQYLHIT
EAEEDVQGTQAAVAALQDLRYTSESGDRLDPTAVNILQQIIELGAETHDATALASVVAMAPGTVTVVKQVTEEEPSSNHTVMIQETVQQA

--------------------------------------------------------------

>25405_25405_18_EFR3A-ZFAT_EFR3A_chr8_132999949_ENST00000519656_ZFAT_chr8_135577664_ENST00000523399_length(amino acids)=958AA_BP=677
MRPRYKRLVDNIFPEDPKDGLVKTDMEKLTFYAVSAPEKLDRIGSYLAERLSRDVVRHRSGYVLIAMEALDQLLMACHSQSIKPFVESFL
HMVAKLLESGEPKLQVLGTNSFVKFANIEEDTPSYHRRYDFFVSRFSAMCHSCHSDPEIRTEIRIAGIRGIQGVVRKTVNDELRATIWEP
QHMDKIVPSLLFNMQKIEEVDSRIGPPSSPSATDKEENPAVLAENCFRELLGRATFGNMNNAVRPVFAHLDHHKLWDPNEFAVHCFKIIM
YSIQAQYSHHVIQEILGHLDARKKDAPRVRAGIIQVLLEAVAIAAKGSIGPTVLEVFNTLLKHLRLSVEFEANDLQGGSVGSVNLNTSSK
DNDEKIVQNAIIQTIGFFGSNLPDYQRSEIMMFIMGKVPVFGTSTHTLDISQLGDLGTRRIQIMLLRSLLMVTSGYKAKTIVTALPGSFL
DPLLSPSLMEDYELRQLVLEVMHNLMDRHDNRAKLRGIRIIPDVADLKIKREKICRQDTSFMKKNGQQLYRHIYLGCKEEDNVQKNYELL
YTSLALITIELANEEVVIDLIRLAIALQDSAIINEDNLPMFHRCGIMALVAAYLNFVSQMIAVPAFCQHVSKVIEIRTMEAPYFLPEHIF
RDKCMLPKSLEKHEKDLYFLTNKIAESLGGSGYSVERLSVPYVPQVTGKQFKCTVCDYTAAQKPQLLRHMEQHVSFKPFRCAHCHYSCNI
SGSLKRHYNRKHPNEEYANVGTGELAAEVLIQQGGLKCPVCSFVYGTKWEFNRHLKNKHGLKVVEIDGDPKWETATEAPEEPSTQYLHIT
EAEEDVQGTQAAVAALQDLRYTSESGDRLDPTAVNILQQIIELGAETHDATALASVVAMAPGTVTVVKQVTEEEPSSNHTVMIQETVQQA

--------------------------------------------------------------

Top

Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr8:132999949/chr8:135577664)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
EFR3A Q14156	.
FUNCTION: Component of a complex required to localize phosphatidylinositol 4-kinase (PI4K) to the plasma membrane (PubMed:23229899, PubMed:25608530, PubMed:26571211). The complex acts as a regulator of phosphatidylinositol 4-phosphate (PtdIns(4)P) synthesis (Probable). In the complex, EFR3A probably acts as the membrane-anchoring component (PubMed:23229899). Also involved in responsiveness to G-protein-coupled receptors; it is however unclear whether this role is direct or indirect (PubMed:25380825). {ECO:0000269\|PubMed:23229899, ECO:0000269\|PubMed:25380825, ECO:0000269\|PubMed:25608530, ECO:0000305}.	FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000377838

1041_1064

962.3333333333334

1244.0

Zinc finger

C2H2-type 19

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000377838

966_988

962.3333333333334

1244.0

Zinc finger

C2H2-type 17

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000377838

994_1017

962.3333333333334

1244.0

Zinc finger

C2H2-type 18

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000520214

1041_1064

950.3333333333334

1232.0

Zinc finger

C2H2-type 19

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000520214

966_988

950.3333333333334

1232.0

Zinc finger

C2H2-type 17

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000520214

994_1017

950.3333333333334

1232.0

Zinc finger

C2H2-type 18

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000520727

1041_1064

950.3333333333334

1232.0

Zinc finger

C2H2-type 19

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000520727

966_988

950.3333333333334

1232.0

Zinc finger

C2H2-type 17

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000520727

994_1017

950.3333333333334

1232.0

Zinc finger

C2H2-type 18

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000523399

1041_1064

900.3333333333334

1182.0

Zinc finger

C2H2-type 19

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000523399

909_931

900.3333333333334

1182.0

Zinc finger

C2H2-type 15

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000523399

937_959

900.3333333333334

1182.0

Zinc finger

C2H2-type 16

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000523399

966_988

900.3333333333334

1182.0

Zinc finger

C2H2-type 17

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000523399

994_1017

900.3333333333334

1182.0

Zinc finger

C2H2-type 18

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000377838

116_141

962.3333333333334

1244.0

Zinc finger

C2H2-type 2%3B degenerate

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000377838

12_35

962.3333333333334

1244.0

Zinc finger

C2H2-type 1

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000377838

271_293

962.3333333333334

1244.0

Zinc finger

C2H2-type 3

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000377838

299_321

962.3333333333334

1244.0

Zinc finger

C2H2-type 4

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000377838

326_349

962.3333333333334

1244.0

Zinc finger

C2H2-type 5

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000377838

354_377

962.3333333333334

1244.0

Zinc finger

C2H2-type 6

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000377838

404_426

962.3333333333334

1244.0

Zinc finger

C2H2-type 7

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000377838

432_454

962.3333333333334

1244.0

Zinc finger

C2H2-type 8

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000377838

458_481

962.3333333333334

1244.0

Zinc finger

C2H2-type 9

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000377838

742_764

962.3333333333334

1244.0

Zinc finger

C2H2-type 10

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000377838

770_793

962.3333333333334

1244.0

Zinc finger

C2H2-type 11

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000377838

798_822

962.3333333333334

1244.0

Zinc finger

C2H2-type 12

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000377838

830_853

962.3333333333334

1244.0

Zinc finger

C2H2-type 13

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000377838

880_903

962.3333333333334

1244.0

Zinc finger

C2H2-type 14%3B degenerate

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000377838

909_931

962.3333333333334

1244.0

Zinc finger

C2H2-type 15

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000377838

937_959

962.3333333333334

1244.0

Zinc finger

C2H2-type 16

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000520214

116_141

950.3333333333334

1232.0

Zinc finger

C2H2-type 2%3B degenerate

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000520214

12_35

950.3333333333334

1232.0

Zinc finger

C2H2-type 1

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000520214

271_293

950.3333333333334

1232.0

Zinc finger

C2H2-type 3

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000520214

299_321

950.3333333333334

1232.0

Zinc finger

C2H2-type 4

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000520214

326_349

950.3333333333334

1232.0

Zinc finger

C2H2-type 5

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000520214

354_377

950.3333333333334

1232.0

Zinc finger

C2H2-type 6

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000520214

404_426

950.3333333333334

1232.0

Zinc finger

C2H2-type 7

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000520214

432_454

950.3333333333334

1232.0

Zinc finger

C2H2-type 8

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000520214

458_481

950.3333333333334

1232.0

Zinc finger

C2H2-type 9

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000520214

742_764

950.3333333333334

1232.0

Zinc finger

C2H2-type 10

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000520214

770_793

950.3333333333334

1232.0

Zinc finger

C2H2-type 11

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000520214

798_822

950.3333333333334

1232.0

Zinc finger

C2H2-type 12

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000520214

830_853

950.3333333333334

1232.0

Zinc finger

C2H2-type 13

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000520214

880_903

950.3333333333334

1232.0

Zinc finger

C2H2-type 14%3B degenerate

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000520214

909_931

950.3333333333334

1232.0

Zinc finger

C2H2-type 15

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000520214

937_959

950.3333333333334

1232.0

Zinc finger

C2H2-type 16

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000520727

116_141

950.3333333333334

1232.0

Zinc finger

C2H2-type 2%3B degenerate

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000520727

12_35

950.3333333333334

1232.0

Zinc finger

C2H2-type 1

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000520727

271_293

950.3333333333334

1232.0

Zinc finger

C2H2-type 3

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000520727

299_321

950.3333333333334

1232.0

Zinc finger

C2H2-type 4

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000520727

326_349

950.3333333333334

1232.0

Zinc finger

C2H2-type 5

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000520727

354_377

950.3333333333334

1232.0

Zinc finger

C2H2-type 6

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000520727

404_426

950.3333333333334

1232.0

Zinc finger

C2H2-type 7

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000520727

432_454

950.3333333333334

1232.0

Zinc finger

C2H2-type 8

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000520727

458_481

950.3333333333334

1232.0

Zinc finger

C2H2-type 9

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000520727

742_764

950.3333333333334

1232.0

Zinc finger

C2H2-type 10

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000520727

770_793

950.3333333333334

1232.0

Zinc finger

C2H2-type 11

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000520727

798_822

950.3333333333334

1232.0

Zinc finger

C2H2-type 12

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000520727

830_853

950.3333333333334

1232.0

Zinc finger

C2H2-type 13

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000520727

880_903

950.3333333333334

1232.0

Zinc finger

C2H2-type 14%3B degenerate

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000520727

909_931

950.3333333333334

1232.0

Zinc finger

C2H2-type 15

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000520727

937_959

950.3333333333334

1232.0

Zinc finger

C2H2-type 16

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000523399

116_141

900.3333333333334

1182.0

Zinc finger

C2H2-type 2%3B degenerate

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000523399

12_35

900.3333333333334

1182.0

Zinc finger

C2H2-type 1

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000523399

271_293

900.3333333333334

1182.0

Zinc finger

C2H2-type 3

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000523399

299_321

900.3333333333334

1182.0

Zinc finger

C2H2-type 4

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000523399

326_349

900.3333333333334

1182.0

Zinc finger

C2H2-type 5

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000523399

354_377

900.3333333333334

1182.0

Zinc finger

C2H2-type 6

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000523399

404_426

900.3333333333334

1182.0

Zinc finger

C2H2-type 7

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000523399

432_454

900.3333333333334

1182.0

Zinc finger

C2H2-type 8

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000523399

458_481

900.3333333333334

1182.0

Zinc finger

C2H2-type 9

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000523399

742_764

900.3333333333334

1182.0

Zinc finger

C2H2-type 10

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000523399

770_793

900.3333333333334

1182.0

Zinc finger

C2H2-type 11

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000523399

798_822

900.3333333333334

1182.0

Zinc finger

C2H2-type 12

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000523399

830_853

900.3333333333334

1182.0

Zinc finger

C2H2-type 13

Tgene

ZFAT

chr8:132999949

chr8:135577664

ENST00000523399

880_903

900.3333333333334

1182.0

Zinc finger

C2H2-type 14%3B degenerate

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Fusion Protein Structures

PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.

Fusion protein PDB link (fusion AA seq ID in FusionPDB)	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	AA seq	Len(AA seq)
PDB file >>>1784_EFR3A_132999949_ZFAT_135577664_ranked_0.pdb	EFR3A	132999949	132999949	ENST00000523399	ZFAT	chr8	135577664	-	MSLGAAASAVEIAMPTRVCCCCSALRPRYKRLVDNIFPEDPKDGLVKTDMEKLTFYAVSAPEKLDRIGSYLAERLSRDVVRHRSGYVLIA MEALDQLLMACHSQSIKPFVESFLHMVAKLLESGEPKLQVLGTNSFVKFANIEEDTPSYHRRYDFFVSRFSAMCHSCHSDPEIRTEIRIA GIRGIQGVVRKTVNDELRATIWEPQHMDKIVPSLLFNMQKIEEVDSRIGPPSSPSATDKEENPAVLAENCFRELLGRATFGNMNNAVRPV FAHLDHHKLWDPNEFAVHCFKIIMYSIQAQYSHHVIQEILGHLDARKKDAPRVRAGIIQVLLEAVAIAAKGSIGPTVLEVFNTLLKHLRL SVEFEANDLQGGSVGSVNLNTSSKDNDEKIVQNAIIQTIGFFGSNLPDYQRSEIMMFIMGKVPVFGTSTHTLDISQLGDLGTRRIQIMLL RSLLMVTSGYKAKTIVTALPGSFLDPLLSPSLMEDYELRQLVLEVMHNLMDRHDNRAKLRGIRIIPDVADLKIKREKICRQDTSFMKKNG QQLYRHIYLGCKEEDNVQKNYELLYTSLALITIELANEEVVIDLIRLAIALQDSAIINEDNLPMFHRCGIMALVAAYLNFVSQMIAVPAF CQHVSKVIEIRTMEAPYFLPEHIFRDKCMLPKSLEKHEKDLYFLTNKIAESLGGSGYSVERLSVPYVPQVTGKQFKCTVCDYTAAQKPQL LRHMEQHVSFKPFRCAHCHYSCNISGSLKRHYNRKHPNEEYANVGTGELAAEVLIQQGGLKCPVCSFVYGTKWEFNRHLKNKHGLKVVEI DGDPKWETATEAPEEPSTQYLHITEAEEDVQGTQAAVAALQDLRYTSESGDRLDPTAVNILQQIIELGAETHDATALASVVAMAPGTVTV	982

Top

pLDDT score distribution

pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

EFR3A_pLDDT.png

ZFAT_pLDDT.png

pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

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Ramachandran Plot of Fusion Protein Structure

Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.

Fusion AA seq ID in FusionPDB and their Ramachandran plots

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Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
EFR3A
ZFAT

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

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Related Drugs to EFR3A-ZFAT

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

Top

Related Diseases to EFR3A-ZFAT

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source