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Fusion Protein:EIF2AK3-MBD2 |
Fusion Protein Summary |
 Fusion gene summary |
| Fusion partner gene information | Fusion gene name: EIF2AK3-MBD2 | FusionPDB ID: 25669 | FusionGDB2.0 ID: 25669 | Hgene | Tgene | Gene symbol | EIF2AK3 | MBD2 | Gene ID | 9451 | 64174 |
| Gene name | eukaryotic translation initiation factor 2 alpha kinase 3 | dipeptidase 2 | |
| Synonyms | PEK|PERK|WRS | MBD2 | |
| Cytomap | 2p11.2 | 16q22.1 | |
| Type of gene | protein-coding | protein-coding | |
| Description | eukaryotic translation initiation factor 2-alpha kinase 3PRKR-like endoplasmic reticulum kinasepancreatic EIF2-alpha kinasetruncated eukaryotic translation initiation factor 2 alpha kinase 3 | dipeptidase 2 | |
| Modification date | 20200320 | 20200313 | |
| UniProtAcc | Q9NZJ5 | Q9UBB5 | |
| Ensembl transtripts involved in fusion gene | ENST ids | ENST00000303236, ENST00000419748, ENST00000470706, | ENST00000398398, ENST00000579025, ENST00000583046, ENST00000256429, |
| Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0) | * DoF score | 6 X 7 X 4=168 | 13 X 7 X 7=637 |
| # samples | 7 | 15 | |
| ** MAII score | log2(7/168*10)=-1.26303440583379 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | log2(15/637*10)=-2.08633087176042 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | |
| Context (manual curation of fusion genes in FusionPDB) | PubMed: EIF2AK3 [Title/Abstract] AND MBD2 [Title/Abstract] AND fusion [Title/Abstract] | ||
| Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0) | EIF2AK3(88913241)-MBD2(51715381), # samples:1 EIF2AK3(88913242)-MBD2(51715381), # samples:1 | ||
| Anticipated loss of major functional domain due to fusion event. | EIF2AK3-MBD2 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. EIF2AK3-MBD2 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. EIF2AK3-MBD2 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. EIF2AK3-MBD2 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. | ||
| * DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
| Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
| Partner | Gene | GO ID | GO term | PubMed ID |
| Hgene | EIF2AK3 | GO:0006983 | ER overload response | 10677345|11907036 |
| Hgene | EIF2AK3 | GO:0030968 | endoplasmic reticulum unfolded protein response | 10677345|11907036|22169477 |
| Hgene | EIF2AK3 | GO:0046777 | protein autophosphorylation | 10026192|11907036 |
| Fusion gene breakpoints across EIF2AK3 (5'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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| Fusion gene breakpoints across MBD2 (3'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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Fusion Gene Sample Information |
| Fusion gene information from FusionGDB2.0. |
| Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0) * All genome coordinats were lifted-over on hg19. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| Source | Disease | Sample | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| ChimerDB4 | STAD | TCGA-BR-8080-01A | EIF2AK3 | chr2 | 88913242 | - | MBD2 | chr18 | 51715381 | - |
| ChimerDB4 | STAD | TCGA-BR-8080 | EIF2AK3 | chr2 | 88913241 | - | MBD2 | chr18 | 51715381 | - |
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Fusion ORF Analysis |
| Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
| ENST00000303236 | EIF2AK3 | chr2 | 88913241 | - | ENST00000256429 | MBD2 | chr18 | 51715381 | - | 3889 | 740 | 227 | 1273 | 348 |
| ENST00000303236 | EIF2AK3 | chr2 | 88913242 | - | ENST00000256429 | MBD2 | chr18 | 51715381 | - | 3889 | 740 | 227 | 1273 | 348 |
| DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
| ENST00000303236 | ENST00000256429 | EIF2AK3 | chr2 | 88913241 | - | MBD2 | chr18 | 51715381 | - | 0.000276105 | 0.99972385 |
| ENST00000303236 | ENST00000256429 | EIF2AK3 | chr2 | 88913242 | - | MBD2 | chr18 | 51715381 | - | 0.000276105 | 0.99972385 |
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Fusion Amino Acid Sequences |
| For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones. |
| >FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP >25669_25669_1_EIF2AK3-MBD2_EIF2AK3_chr2_88913241_ENST00000303236_MBD2_chr18_51715381_ENST00000256429_length(amino acids)=348AA_BP=171 MGAPSGGRGRRQWLRLHACARGGTLMERAISPGLLVRALLLLLLLLGLAARTVAAGRARGLPAPTAEAAFGLGAAAAPTSATRVPAAGAV AAAEVTVEDAEALPAAAGEQEPRGPEPDDETELRPRGRSLVIISTLDGRIAALDPENHGKKQWDLDVGSGSLVSSSLSKPEGKPDLNTTL PIRQTASIFKQPVTKVTNHPSNKVKSDPQRMNEQPRQLFWEKRLQGLSASDVTEQIIKTMELPKGLQGVGPGSNDETLLSAVASALHTSS -------------------------------------------------------------- >25669_25669_2_EIF2AK3-MBD2_EIF2AK3_chr2_88913242_ENST00000303236_MBD2_chr18_51715381_ENST00000256429_length(amino acids)=348AA_BP=171 MGAPSGGRGRRQWLRLHACARGGTLMERAISPGLLVRALLLLLLLLGLAARTVAAGRARGLPAPTAEAAFGLGAAAAPTSATRVPAAGAV AAAEVTVEDAEALPAAAGEQEPRGPEPDDETELRPRGRSLVIISTLDGRIAALDPENHGKKQWDLDVGSGSLVSSSLSKPEGKPDLNTTL PIRQTASIFKQPVTKVTNHPSNKVKSDPQRMNEQPRQLFWEKRLQGLSASDVTEQIIKTMELPKGLQGVGPGSNDETLLSAVASALHTSS -------------------------------------------------------------- |
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Fusion Protein Functional Features |
| Four levels of functional features of fusion genes Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr2:88913241/chr18:51715381) - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. - How to search 1. Put your fusion gene symbol. 2. Press the tab key until there will be shown the breakpoint information filled. 4. Go down and press 'Search' tab twice. 4. Go down to have the hyperlink of the search result. 5. Click the hyperlink. 6. See the FGviewer result for your fusion gene. |
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| Main function of each fusion partner protein. (from UniProt) |
| Hgene | Tgene |
| EIF2AK3 | MBD2 |
| FUNCTION: Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) in response to various stress conditions. Key activator of the integrated stress response (ISR) required for adaptation to various stress, such as unfolded protein response (UPR) and low amino acid availability. EIF2S1/eIF-2-alpha phosphorylation in response to stress converts EIF2S1/eIF-2-alpha in a global protein synthesis inhibitor, leading to a global attenuation of cap-dependent translation, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function. {ECO:0000250|UniProtKB:Q9Z2B5}. | FUNCTION: Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression. May enhance the activation of some unmethylated cAMP-responsive promoters. {ECO:0000269|PubMed:10471499, ECO:0000269|PubMed:10947852, ECO:0000269|PubMed:12665568, ECO:0000269|PubMed:16415179, ECO:0000269|PubMed:24307175, ECO:0000269|PubMed:9774669}. |
| Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at * Minus value of BPloci means that the break pointn is located before the CDS. |
| - Retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| Hgene | EIF2AK3 | chr2:88913241 | chr18:51715381 | ENST00000303236 | - | 2 | 17 | 49_52 | 146.0 | 1117.0 | Compositional bias | Note=Poly-Ala |
| Hgene | EIF2AK3 | chr2:88913242 | chr18:51715381 | ENST00000303236 | - | 2 | 17 | 49_52 | 146.0 | 1117.0 | Compositional bias | Note=Poly-Ala |
| Tgene | MBD2 | chr2:88913241 | chr18:51715381 | ENST00000583046 | 0 | 3 | 50_96 | 0 | 303.0 | Compositional bias | Note=Arg-rich | |
| Tgene | MBD2 | chr2:88913241 | chr18:51715381 | ENST00000583046 | 0 | 3 | 6_140 | 0 | 303.0 | Compositional bias | Note=Gly-rich | |
| Tgene | MBD2 | chr2:88913242 | chr18:51715381 | ENST00000583046 | 0 | 3 | 50_96 | 0 | 303.0 | Compositional bias | Note=Arg-rich | |
| Tgene | MBD2 | chr2:88913242 | chr18:51715381 | ENST00000583046 | 0 | 3 | 6_140 | 0 | 303.0 | Compositional bias | Note=Gly-rich | |
| Tgene | MBD2 | chr2:88913241 | chr18:51715381 | ENST00000583046 | 0 | 3 | 145_213 | 0 | 303.0 | Domain | MBD | |
| Tgene | MBD2 | chr2:88913242 | chr18:51715381 | ENST00000583046 | 0 | 3 | 145_213 | 0 | 303.0 | Domain | MBD |
| - Not-retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| Hgene | EIF2AK3 | chr2:88913241 | chr18:51715381 | ENST00000303236 | - | 2 | 17 | 593_1077 | 146.0 | 1117.0 | Domain | Protein kinase |
| Hgene | EIF2AK3 | chr2:88913242 | chr18:51715381 | ENST00000303236 | - | 2 | 17 | 593_1077 | 146.0 | 1117.0 | Domain | Protein kinase |
| Hgene | EIF2AK3 | chr2:88913241 | chr18:51715381 | ENST00000303236 | - | 2 | 17 | 599_607 | 146.0 | 1117.0 | Nucleotide binding | ATP |
| Hgene | EIF2AK3 | chr2:88913242 | chr18:51715381 | ENST00000303236 | - | 2 | 17 | 599_607 | 146.0 | 1117.0 | Nucleotide binding | ATP |
| Hgene | EIF2AK3 | chr2:88913241 | chr18:51715381 | ENST00000303236 | - | 2 | 17 | 30_514 | 146.0 | 1117.0 | Topological domain | Lumenal |
| Hgene | EIF2AK3 | chr2:88913241 | chr18:51715381 | ENST00000303236 | - | 2 | 17 | 536_1116 | 146.0 | 1117.0 | Topological domain | Cytoplasmic |
| Hgene | EIF2AK3 | chr2:88913242 | chr18:51715381 | ENST00000303236 | - | 2 | 17 | 30_514 | 146.0 | 1117.0 | Topological domain | Lumenal |
| Hgene | EIF2AK3 | chr2:88913242 | chr18:51715381 | ENST00000303236 | - | 2 | 17 | 536_1116 | 146.0 | 1117.0 | Topological domain | Cytoplasmic |
| Hgene | EIF2AK3 | chr2:88913241 | chr18:51715381 | ENST00000303236 | - | 2 | 17 | 515_535 | 146.0 | 1117.0 | Transmembrane | Helical |
| Hgene | EIF2AK3 | chr2:88913242 | chr18:51715381 | ENST00000303236 | - | 2 | 17 | 515_535 | 146.0 | 1117.0 | Transmembrane | Helical |
| Tgene | MBD2 | chr2:88913241 | chr18:51715381 | ENST00000256429 | 1 | 7 | 50_96 | 234.0 | 1015.6666666666666 | Compositional bias | Note=Arg-rich | |
| Tgene | MBD2 | chr2:88913241 | chr18:51715381 | ENST00000256429 | 1 | 7 | 6_140 | 234.0 | 1015.6666666666666 | Compositional bias | Note=Gly-rich | |
| Tgene | MBD2 | chr2:88913242 | chr18:51715381 | ENST00000256429 | 1 | 7 | 50_96 | 234.0 | 1015.6666666666666 | Compositional bias | Note=Arg-rich | |
| Tgene | MBD2 | chr2:88913242 | chr18:51715381 | ENST00000256429 | 1 | 7 | 6_140 | 234.0 | 1015.6666666666666 | Compositional bias | Note=Gly-rich | |
| Tgene | MBD2 | chr2:88913241 | chr18:51715381 | ENST00000256429 | 1 | 7 | 145_213 | 234.0 | 1015.6666666666666 | Domain | MBD | |
| Tgene | MBD2 | chr2:88913242 | chr18:51715381 | ENST00000256429 | 1 | 7 | 145_213 | 234.0 | 1015.6666666666666 | Domain | MBD |
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Fusion Protein-Protein Interaction |
| Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in |
| Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160) |
| Gene | PPI interactors |
| Protein-protein interactors based on sequence similarity (STRING) |
| Gene | STRING network |
| EIF2AK3 | |
| MBD2 |
| - Retained interactions in fusion protein (protein functional feature from UniProt). |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Still interaction with |
| - Lost interactions due to fusion (protein functional feature from UniProt). |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
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Related Drugs to EIF2AK3-MBD2 |
| Drugs used for this fusion-positive patient. (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
| Hgene | Tgene | Drug | Source | PMID |
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Related Diseases to EIF2AK3-MBD2 |
| Diseases that have this fusion gene. (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
| Hgene | Tgene | Disease | Source | PMID |
| Diseases associated with fusion partners. (DisGeNet 4.0) |
| Partner | Gene | Disease ID | Disease name | # pubmeds | Source |
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