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	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein Structure
	pLDDT scores
	Ramachandran Plot of Fusion Protein Structure
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:EML1-CDC27

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: EML1-CDC27
	FusionPDB ID: 26428
	FusionGDB2.0 ID: 26428
		Hgene	Tgene
	Gene symbol	EML1	CDC27
	Gene ID	2009	996
	Gene name	EMAP like 1	cell division cycle 27
	Synonyms	BH\|ELP79\|EMAP\|EMAP-1\|EMAPL	ANAPC3\|APC3\|CDC27Hs\|D0S1430E\|D17S978E\|H-NUC\|HNUC\|NUC2
	Cytomap	14q32.2	17q21.32
	Type of gene	protein-coding	protein-coding
	Description	echinoderm microtubule-associated protein-like 1echinoderm microtubule associated protein like 1	cell division cycle protein 27 homologD0S1430E, D17S978Eanaphase promoting complex subunit 3anaphase-promoting complex, protein 3cell division cycle 27 homolognuc2 homolog
	Modification date	20200313	20200313
	UniProtAcc	O00423	P30260
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000262233, ENST00000327921, ENST00000334192, ENST00000556758,	ENST00000528748, ENST00000446365, ENST00000527547, ENST00000066544, ENST00000531206,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	15 X 8 X 11=1320	7 X 7 X 4=196
	# samples	17	8
	** MAII score	log2(17/1320*10)=-2.95693127810811 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(8/196*10)=-1.29278174922785 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)	PubMed: EML1 [Title/Abstract] AND CDC27 [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	EML1(100387214)-CDC27(45249430), # samples:2
Anticipated loss of major functional domain due to fusion event.	EML1-CDC27 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. EML1-CDC27 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. EML1-CDC27 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. EML1-CDC27 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Tgene	CDC27	GO:0070979	protein K11-linked ubiquitination	18485873

Fusion gene breakpoints across EML1 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across CDC27 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	UCEC	TCGA-A5-A3LP-01A	EML1	chr14	100387214	+	CDC27	chr17	45249430	-
ChimerDB4	UCEC	TCGA-A5-A3LP	EML1	chr14	100387214	+	CDC27	chr17	45249430	-

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000327921	EML1	chr14	100387214	+	ENST00000066544	CDC27	chr17	45249430	-	8568	2964	974	5335	1453
ENST00000327921	EML1	chr14	100387214	+	ENST00000531206	CDC27	chr17	45249430	-	6034	2964	974	5353	1459
ENST00000262233	EML1	chr14	100387214	+	ENST00000066544	CDC27	chr17	45249430	-	7652	2048	139	4419	1426
ENST00000262233	EML1	chr14	100387214	+	ENST00000531206	CDC27	chr17	45249430	-	5118	2048	139	4437	1432
ENST00000334192	EML1	chr14	100387214	+	ENST00000066544	CDC27	chr17	45249430	-	7704	2100	134	4471	1445
ENST00000334192	EML1	chr14	100387214	+	ENST00000531206	CDC27	chr17	45249430	-	5170	2100	134	4489	1451

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000327921	ENST00000066544	EML1	chr14	100387214	+	CDC27	chr17	45249430	-	0.000490351	0.9995097
ENST00000327921	ENST00000531206	EML1	chr14	100387214	+	CDC27	chr17	45249430	-	0.001255818	0.99874413
ENST00000262233	ENST00000066544	EML1	chr14	100387214	+	CDC27	chr17	45249430	-	0.000137332	0.9998627
ENST00000262233	ENST00000531206	EML1	chr14	100387214	+	CDC27	chr17	45249430	-	0.000409591	0.99959046
ENST00000334192	ENST00000066544	EML1	chr14	100387214	+	CDC27	chr17	45249430	-	0.000140851	0.9998591
ENST00000334192	ENST00000531206	EML1	chr14	100387214	+	CDC27	chr17	45249430	-	0.000417019	0.99958295

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>26428_26428_1_EML1-CDC27_EML1_chr14_100387214_ENST00000262233_CDC27_chr17_45249430_ENST00000066544_length(amino acids)=1426AA_BP=636
MEDGFSSYSSLYDTSSLLQFCNDDSASAASSMEVTDRIASLEQRVQMQEDDIQLLKSALADVVRRLNITEEQQAVLNRKGPTKARPLMQT
LPLRTTVNNGTVLPKKPTGSLPSPSGVRKETAVPATKSNIKRTSSSERVSPGGRRESNGDSRGNRNRTGSTSSSSSGKKNSESKPKEPVF
SAEEGYVKMFLRGRPVTMYMPKDQVDSYSLEAKVELPTKRLKLEWVYGYRGRDCRNNLYLLPTGETVYFIASVVVLYNVEEQLQRHYAGH
NDDVKCLAVHPDRITIATGQVAGTSKDGKQLPPHVRIWDSVTLNTLHVIGIGFFDRAVTCIAFSKSNGGTNLCAVDDSNDHVLSVWDWQK
EEKLADVKCSNEAVFAADFHPTDTNIIVTCGKSHLYFWTLEGSSLNKKQGLFEKQEKPKFVLCVTFSENGDTITGDSSGNILVWGKGTNR
ISYAVQGAHEGGIFALCMLRDGTLVSGGGKDRKLISWSGNYQKLRKTEIPEQFGPIRTVAEGKGDVILIGTTRNFVLQGTLSGDFTPITQ
GHTDELWGLAIHASKSQFLTCGHDKHATLWDAVGHRPVWDKIIEDPAQSSGFHPSGSVVAVGTLTGRWFVFDTETKDLVTVHTDGNEQLS
VMRYSPVHSEEALFLLATCYYRSGKAYKAYRLLKGHSCTTPQCKYLLAKCCVDLSKLAEGEQILSGGVFNKQKSHDDIVTEFGDSACFTL
SLLGHVYCKTDRLAKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKFTSLQNFSNCLPNSCTTQVPNHSLSHRQPETVLTETPQD
TIELNRLNLESSNSKYSLNTDSSVSYIDSAVISPDTVPLGTGTSILSKQVQNKPKTGRSLLGGPAALSPLTPSFGILPLETPSPGDGSYL
QNYTNTPPVIDVPSTGAPSKKSVARIGQTGTKSVFSQSGNSREVTPILAQTQSSGPQTSTTPQVLSPTITSPPNALPRRSSRLFTSDSST
TKENSKKLKMKFPPKIPNRKTKSKTNKGGITQPNINDSLEITKLDSSIISEGKISTITPQIQAFNLQKAAAEGLMSLLREMGKGYLALCS
YNCKEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYSTTLWHLQKDVALSVLSKDLTDMDKNSPE
AWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHF
QKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKVYK

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>26428_26428_2_EML1-CDC27_EML1_chr14_100387214_ENST00000262233_CDC27_chr17_45249430_ENST00000531206_length(amino acids)=1432AA_BP=636
MEDGFSSYSSLYDTSSLLQFCNDDSASAASSMEVTDRIASLEQRVQMQEDDIQLLKSALADVVRRLNITEEQQAVLNRKGPTKARPLMQT
LPLRTTVNNGTVLPKKPTGSLPSPSGVRKETAVPATKSNIKRTSSSERVSPGGRRESNGDSRGNRNRTGSTSSSSSGKKNSESKPKEPVF
SAEEGYVKMFLRGRPVTMYMPKDQVDSYSLEAKVELPTKRLKLEWVYGYRGRDCRNNLYLLPTGETVYFIASVVVLYNVEEQLQRHYAGH
NDDVKCLAVHPDRITIATGQVAGTSKDGKQLPPHVRIWDSVTLNTLHVIGIGFFDRAVTCIAFSKSNGGTNLCAVDDSNDHVLSVWDWQK
EEKLADVKCSNEAVFAADFHPTDTNIIVTCGKSHLYFWTLEGSSLNKKQGLFEKQEKPKFVLCVTFSENGDTITGDSSGNILVWGKGTNR
ISYAVQGAHEGGIFALCMLRDGTLVSGGGKDRKLISWSGNYQKLRKTEIPEQFGPIRTVAEGKGDVILIGTTRNFVLQGTLSGDFTPITQ
GHTDELWGLAIHASKSQFLTCGHDKHATLWDAVGHRPVWDKIIEDPAQSSGFHPSGSVVAVGTLTGRWFVFDTETKDLVTVHTDGNEQLS
VMRYSPVHSEEALFLLATCYYRSGKAYKAYRLLKGHSCTTPQCKYLLAKCCVDLSKLAEGEQILSGGVFNKQKSHDDIVTEFGDSACFTL
SLLGHVYCKTDRLAKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKFTSLQNFSNCLPNSCTTQVPNHSLSHRQPETVLTETPQD
TIELNRLNLESSNSKYSLNTDSSVSYIDSAVISPDTVPLGTGTSILSKQVQNKPKTGRSLLGGPAALSPLTPSFGILPLETPSPGDGSYL
QNYTNTPPVIDVPSTGAPSKKTFRVLQSVARIGQTGTKSVFSQSGNSREVTPILAQTQSSGPQTSTTPQVLSPTITSPPNALPRRSSRLF
TSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKGGITQPNINDSLEITKLDSSIISEGKISTITPQIQAFNLQKAAAEGLMSLLREMGKG
YLALCSYNCKEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYSTTLWHLQKDVALSVLSKDLTDM
DKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFS
LAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFL

--------------------------------------------------------------

>26428_26428_3_EML1-CDC27_EML1_chr14_100387214_ENST00000327921_CDC27_chr17_45249430_ENST00000066544_length(amino acids)=1453AA_BP=663
MPPASPLGGGAAGRLTQGRPGLLSSASRRLDDSASAASSMEVTDRIASLEQRVQMQEDDIQLLKSALADVVRRLNITEEQQAVLNRKGPT
KARPLMQTLPLRTTVNNGTVLPKKPTGSLPSPSGVRKETAVPATKRLNRSVSLLNACKLNRSTPSNIKRTSSSERVSPGGRRESNGDSRG
NRNRTGSTSSSSSGKKNSESKPKEPVFSAEEGYVKMFLRGRPVTMYMPKDQVDSYSLEAKVELPTKRLKLEWVYGYRGRDCRNNLYLLPT
GETVYFIASVVVLYNVEEQLQRHYAGHNDDVKCLAVHPDRITIATGQVAGTSKDGKQLPPHVRIWDSVTLNTLHVIGIGFFDRAVTCIAF
SKSNGGTNLCAVDDSNDHVLSVWDWQKEEKLADVKCSNEAVFAADFHPTDTNIIVTCGKSHLYFWTLEGSSLNKKQGLFEKQEKPKFVLC
VTFSENGDTITGDSSGNILVWGKGTNRISYAVQGAHEGGIFALCMLRDGTLVSGGGKDRKLISWSGNYQKLRKTEIPEQFGPIRTVAEGK
GDVILIGTTRNFVLQGTLSGDFTPITQGHTDELWGLAIHASKSQFLTCGHDKHATLWDAVGHRPVWDKIIEDPAQSSGFHPSGSVVAVGT
LTGRWFVFDTETKDLVTVHTDGNEQLSVMRYSPVHSEEALFLLATCYYRSGKAYKAYRLLKGHSCTTPQCKYLLAKCCVDLSKLAEGEQI
LSGGVFNKQKSHDDIVTEFGDSACFTLSLLGHVYCKTDRLAKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKFTSLQNFSNCLP
NSCTTQVPNHSLSHRQPETVLTETPQDTIELNRLNLESSNSKYSLNTDSSVSYIDSAVISPDTVPLGTGTSILSKQVQNKPKTGRSLLGG
PAALSPLTPSFGILPLETPSPGDGSYLQNYTNTPPVIDVPSTGAPSKKSVARIGQTGTKSVFSQSGNSREVTPILAQTQSSGPQTSTTPQ
VLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKGGITQPNINDSLEITKLDSSIISEGKISTITPQIQA
FNLQKAAAEGLMSLLREMGKGYLALCSYNCKEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYST
TLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRVN
PRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYKS
ALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNFSWAMDLDPKGANNQIKEAIDKRYLPDDEEPITQEEQIMGTDESQESSMTDA

--------------------------------------------------------------

>26428_26428_4_EML1-CDC27_EML1_chr14_100387214_ENST00000327921_CDC27_chr17_45249430_ENST00000531206_length(amino acids)=1459AA_BP=663
MPPASPLGGGAAGRLTQGRPGLLSSASRRLDDSASAASSMEVTDRIASLEQRVQMQEDDIQLLKSALADVVRRLNITEEQQAVLNRKGPT
KARPLMQTLPLRTTVNNGTVLPKKPTGSLPSPSGVRKETAVPATKRLNRSVSLLNACKLNRSTPSNIKRTSSSERVSPGGRRESNGDSRG
NRNRTGSTSSSSSGKKNSESKPKEPVFSAEEGYVKMFLRGRPVTMYMPKDQVDSYSLEAKVELPTKRLKLEWVYGYRGRDCRNNLYLLPT
GETVYFIASVVVLYNVEEQLQRHYAGHNDDVKCLAVHPDRITIATGQVAGTSKDGKQLPPHVRIWDSVTLNTLHVIGIGFFDRAVTCIAF
SKSNGGTNLCAVDDSNDHVLSVWDWQKEEKLADVKCSNEAVFAADFHPTDTNIIVTCGKSHLYFWTLEGSSLNKKQGLFEKQEKPKFVLC
VTFSENGDTITGDSSGNILVWGKGTNRISYAVQGAHEGGIFALCMLRDGTLVSGGGKDRKLISWSGNYQKLRKTEIPEQFGPIRTVAEGK
GDVILIGTTRNFVLQGTLSGDFTPITQGHTDELWGLAIHASKSQFLTCGHDKHATLWDAVGHRPVWDKIIEDPAQSSGFHPSGSVVAVGT
LTGRWFVFDTETKDLVTVHTDGNEQLSVMRYSPVHSEEALFLLATCYYRSGKAYKAYRLLKGHSCTTPQCKYLLAKCCVDLSKLAEGEQI
LSGGVFNKQKSHDDIVTEFGDSACFTLSLLGHVYCKTDRLAKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKFTSLQNFSNCLP
NSCTTQVPNHSLSHRQPETVLTETPQDTIELNRLNLESSNSKYSLNTDSSVSYIDSAVISPDTVPLGTGTSILSKQVQNKPKTGRSLLGG
PAALSPLTPSFGILPLETPSPGDGSYLQNYTNTPPVIDVPSTGAPSKKTFRVLQSVARIGQTGTKSVFSQSGNSREVTPILAQTQSSGPQ
TSTTPQVLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKGGITQPNINDSLEITKLDSSIISEGKISTI
TPQIQAFNLQKAAAEGLMSLLREMGKGYLALCSYNCKEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEG
MEIYSTTLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFR
NAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFA
NEKYKSALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNFSWAMDLDPKGANNQIKEAIDKRYLPDDEEPITQEEQIMGTDESQE

--------------------------------------------------------------

>26428_26428_5_EML1-CDC27_EML1_chr14_100387214_ENST00000334192_CDC27_chr17_45249430_ENST00000066544_length(amino acids)=1445AA_BP=655
MEDGFSSYSSLYDTSSLLQFCNDDSASAASSMEVTDRIASLEQRVQMQEDDIQLLKSALADVVRRLNITEEQQAVLNRKGPTKARPLMQT
LPLRTTVNNGTVLPKKPTGSLPSPSGVRKETAVPATKRLNRSVSLLNACKLNRSTPSNIKRTSSSERVSPGGRRESNGDSRGNRNRTGST
SSSSSGKKNSESKPKEPVFSAEEGYVKMFLRGRPVTMYMPKDQVDSYSLEAKVELPTKRLKLEWVYGYRGRDCRNNLYLLPTGETVYFIA
SVVVLYNVEEQLQRHYAGHNDDVKCLAVHPDRITIATGQVAGTSKDGKQLPPHVRIWDSVTLNTLHVIGIGFFDRAVTCIAFSKSNGGTN
LCAVDDSNDHVLSVWDWQKEEKLADVKCSNEAVFAADFHPTDTNIIVTCGKSHLYFWTLEGSSLNKKQGLFEKQEKPKFVLCVTFSENGD
TITGDSSGNILVWGKGTNRISYAVQGAHEGGIFALCMLRDGTLVSGGGKDRKLISWSGNYQKLRKTEIPEQFGPIRTVAEGKGDVILIGT
TRNFVLQGTLSGDFTPITQGHTDELWGLAIHASKSQFLTCGHDKHATLWDAVGHRPVWDKIIEDPAQSSGFHPSGSVVAVGTLTGRWFVF
DTETKDLVTVHTDGNEQLSVMRYSPVHSEEALFLLATCYYRSGKAYKAYRLLKGHSCTTPQCKYLLAKCCVDLSKLAEGEQILSGGVFNK
QKSHDDIVTEFGDSACFTLSLLGHVYCKTDRLAKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKFTSLQNFSNCLPNSCTTQVP
NHSLSHRQPETVLTETPQDTIELNRLNLESSNSKYSLNTDSSVSYIDSAVISPDTVPLGTGTSILSKQVQNKPKTGRSLLGGPAALSPLT
PSFGILPLETPSPGDGSYLQNYTNTPPVIDVPSTGAPSKKSVARIGQTGTKSVFSQSGNSREVTPILAQTQSSGPQTSTTPQVLSPTITS
PPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKGGITQPNINDSLEITKLDSSIISEGKISTITPQIQAFNLQKAAA
EGLMSLLREMGKGYLALCSYNCKEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYSTTLWHLQKD
VALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWY
GLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEEL
KQIVPKESLVYFLIGKVYKKLGQTHLALMNFSWAMDLDPKGANNQIKEAIDKRYLPDDEEPITQEEQIMGTDESQESSMTDADDTQLHAA

--------------------------------------------------------------

>26428_26428_6_EML1-CDC27_EML1_chr14_100387214_ENST00000334192_CDC27_chr17_45249430_ENST00000531206_length(amino acids)=1451AA_BP=655
MEDGFSSYSSLYDTSSLLQFCNDDSASAASSMEVTDRIASLEQRVQMQEDDIQLLKSALADVVRRLNITEEQQAVLNRKGPTKARPLMQT
LPLRTTVNNGTVLPKKPTGSLPSPSGVRKETAVPATKRLNRSVSLLNACKLNRSTPSNIKRTSSSERVSPGGRRESNGDSRGNRNRTGST
SSSSSGKKNSESKPKEPVFSAEEGYVKMFLRGRPVTMYMPKDQVDSYSLEAKVELPTKRLKLEWVYGYRGRDCRNNLYLLPTGETVYFIA
SVVVLYNVEEQLQRHYAGHNDDVKCLAVHPDRITIATGQVAGTSKDGKQLPPHVRIWDSVTLNTLHVIGIGFFDRAVTCIAFSKSNGGTN
LCAVDDSNDHVLSVWDWQKEEKLADVKCSNEAVFAADFHPTDTNIIVTCGKSHLYFWTLEGSSLNKKQGLFEKQEKPKFVLCVTFSENGD
TITGDSSGNILVWGKGTNRISYAVQGAHEGGIFALCMLRDGTLVSGGGKDRKLISWSGNYQKLRKTEIPEQFGPIRTVAEGKGDVILIGT
TRNFVLQGTLSGDFTPITQGHTDELWGLAIHASKSQFLTCGHDKHATLWDAVGHRPVWDKIIEDPAQSSGFHPSGSVVAVGTLTGRWFVF
DTETKDLVTVHTDGNEQLSVMRYSPVHSEEALFLLATCYYRSGKAYKAYRLLKGHSCTTPQCKYLLAKCCVDLSKLAEGEQILSGGVFNK
QKSHDDIVTEFGDSACFTLSLLGHVYCKTDRLAKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKFTSLQNFSNCLPNSCTTQVP
NHSLSHRQPETVLTETPQDTIELNRLNLESSNSKYSLNTDSSVSYIDSAVISPDTVPLGTGTSILSKQVQNKPKTGRSLLGGPAALSPLT
PSFGILPLETPSPGDGSYLQNYTNTPPVIDVPSTGAPSKKTFRVLQSVARIGQTGTKSVFSQSGNSREVTPILAQTQSSGPQTSTTPQVL
SPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKGGITQPNINDSLEITKLDSSIISEGKISTITPQIQAFN
LQKAAAEGLMSLLREMGKGYLALCSYNCKEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYSTTL
WHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPR
HYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYKSAL
QELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNFSWAMDLDPKGANNQIKEAIDKRYLPDDEEPITQEEQIMGTDESQESSMTDADD

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Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr14:100387214/chr17:45249430)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
EML1 O00423	CDC27 P30260
FUNCTION: Modulates the assembly and organization of the microtubule cytoskeleton, and probably plays a role in regulating the orientation of the mitotic spindle and the orientation of the plane of cell division. Required for normal proliferation of neuronal progenitor cells in the developing brain and for normal brain development. Does not affect neuron migration per se. {ECO:0000250\|UniProtKB:Q05BC3}.	FUNCTION: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. {ECO:0000269\|PubMed:18485873}.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

EML1

chr14:100387214

chr17:45249430

ENST00000262233

160_166

636.3333333333334

816.0

Compositional bias

Note=Poly-Ser

Hgene

EML1

chr14:100387214

chr17:45249430

ENST00000334192

160_166

655.3333333333334

835.0

Compositional bias

Note=Poly-Ser

Hgene

EML1

chr14:100387214

chr17:45249430

ENST00000262233

261_310

636.3333333333334

816.0

Repeat

Note=WD 1

Hgene

EML1

chr14:100387214

chr17:45249430

ENST00000262233

315_358

636.3333333333334

816.0

Repeat

Note=WD 2

Hgene

EML1

chr14:100387214

chr17:45249430

ENST00000262233

363_400

636.3333333333334

816.0

Repeat

Note=WD 3

Hgene

EML1

chr14:100387214

chr17:45249430

ENST00000262233

409_446

636.3333333333334

816.0

Repeat

Note=WD 4

Hgene

EML1

chr14:100387214

chr17:45249430

ENST00000262233

450_489

636.3333333333334

816.0

Repeat

Note=WD 5

Hgene

EML1

chr14:100387214

chr17:45249430

ENST00000262233

493_530

636.3333333333334

816.0

Repeat

Note=WD 6

Hgene

EML1

chr14:100387214

chr17:45249430

ENST00000262233

535_572

636.3333333333334

816.0

Repeat

Note=WD 7

Hgene

EML1

chr14:100387214

chr17:45249430

ENST00000262233

578_613

636.3333333333334

816.0

Repeat

Note=WD 8

Hgene

EML1

chr14:100387214

chr17:45249430

ENST00000334192

261_310

655.3333333333334

835.0

Repeat

Note=WD 1

Hgene

EML1

chr14:100387214

chr17:45249430

ENST00000334192

315_358

655.3333333333334

835.0

Repeat

Note=WD 2

Hgene

EML1

chr14:100387214

chr17:45249430

ENST00000334192

363_400

655.3333333333334

835.0

Repeat

Note=WD 3

Hgene

EML1

chr14:100387214

chr17:45249430

ENST00000334192

409_446

655.3333333333334

835.0

Repeat

Note=WD 4

Hgene

EML1

chr14:100387214

chr17:45249430

ENST00000334192

450_489

655.3333333333334

835.0

Repeat

Note=WD 5

Hgene

EML1

chr14:100387214

chr17:45249430

ENST00000334192

493_530

655.3333333333334

835.0

Repeat

Note=WD 6

Hgene

EML1

chr14:100387214

chr17:45249430

ENST00000334192

535_572

655.3333333333334

835.0

Repeat

Note=WD 7

Hgene

EML1

chr14:100387214

chr17:45249430

ENST00000334192

578_613

655.3333333333334

835.0

Repeat

Note=WD 8

Hgene

EML1

chr14:100387214

chr17:45249430

ENST00000334192

617_655

655.3333333333334

835.0

Repeat

Note=WD 9

Tgene

CDC27

chr14:100387214

chr17:45249430

ENST00000066544

115_145

34.333333333333336

825.0

Repeat

Note=TPR 4

Tgene

CDC27

chr14:100387214

chr17:45249430

ENST00000066544

38_65

34.333333333333336

825.0

Repeat

Note=TPR 2

Tgene

CDC27

chr14:100387214

chr17:45249430

ENST00000066544

465_495

34.333333333333336

825.0

Repeat

Note=TPR 5

Tgene

CDC27

chr14:100387214

chr17:45249430

ENST00000066544

499_528

34.333333333333336

825.0

Repeat

Note=TPR 6

Tgene

CDC27

chr14:100387214

chr17:45249430

ENST00000066544

533_563

34.333333333333336

825.0

Repeat

Note=TPR 7

Tgene

CDC27

chr14:100387214

chr17:45249430

ENST00000066544

567_598

34.333333333333336

825.0

Repeat

Note=TPR 8

Tgene

CDC27

chr14:100387214

chr17:45249430

ENST00000066544

601_631

34.333333333333336

825.0

Repeat

Note=TPR 9

Tgene

CDC27

chr14:100387214

chr17:45249430

ENST00000066544

635_667

34.333333333333336

825.0

Repeat

Note=TPR 10

Tgene

CDC27

chr14:100387214

chr17:45249430

ENST00000066544

670_702

34.333333333333336

825.0

Repeat

Note=TPR 11

Tgene

CDC27

chr14:100387214

chr17:45249430

ENST00000066544

67_99

34.333333333333336

825.0

Repeat

Note=TPR 3

Tgene

CDC27

chr14:100387214

chr17:45249430

ENST00000066544

704_734

34.333333333333336

825.0

Repeat

Note=TPR 12

Tgene

CDC27

chr14:100387214

chr17:45249430

ENST00000066544

737_768

34.333333333333336

825.0

Repeat

Note=TPR 13

Tgene

CDC27

chr14:100387214

chr17:45249430

ENST00000531206

115_145

34.333333333333336

831.0

Repeat

Note=TPR 4

Tgene

CDC27

chr14:100387214

chr17:45249430

ENST00000531206

38_65

34.333333333333336

831.0

Repeat

Note=TPR 2

Tgene

CDC27

chr14:100387214

chr17:45249430

ENST00000531206

465_495

34.333333333333336

831.0

Repeat

Note=TPR 5

Tgene

CDC27

chr14:100387214

chr17:45249430

ENST00000531206

499_528

34.333333333333336

831.0

Repeat

Note=TPR 6

Tgene

CDC27

chr14:100387214

chr17:45249430

ENST00000531206

533_563

34.333333333333336

831.0

Repeat

Note=TPR 7

Tgene

CDC27

chr14:100387214

chr17:45249430

ENST00000531206

567_598

34.333333333333336

831.0

Repeat

Note=TPR 8

Tgene

CDC27

chr14:100387214

chr17:45249430

ENST00000531206

601_631

34.333333333333336

831.0

Repeat

Note=TPR 9

Tgene

CDC27

chr14:100387214

chr17:45249430

ENST00000531206

635_667

34.333333333333336

831.0

Repeat

Note=TPR 10

Tgene

CDC27

chr14:100387214

chr17:45249430

ENST00000531206

670_702

34.333333333333336

831.0

Repeat

Note=TPR 11

Tgene

CDC27

chr14:100387214

chr17:45249430

ENST00000531206

67_99

34.333333333333336

831.0

Repeat

Note=TPR 3

Tgene

CDC27

chr14:100387214

chr17:45249430

ENST00000531206

704_734

34.333333333333336

831.0

Repeat

Note=TPR 12

Tgene

CDC27

chr14:100387214

chr17:45249430

ENST00000531206

737_768

34.333333333333336

831.0

Repeat

Note=TPR 13

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

EML1

chr14:100387214

chr17:45249430

ENST00000262233

176_815

636.3333333333334

816.0

Region

Note=Tandem atypical propeller in EMLs

Hgene

EML1

chr14:100387214

chr17:45249430

ENST00000334192

176_815

655.3333333333334

835.0

Region

Note=Tandem atypical propeller in EMLs

Hgene

EML1

chr14:100387214

chr17:45249430

ENST00000262233

617_655

636.3333333333334

816.0

Repeat

Note=WD 9

Hgene

EML1

chr14:100387214

chr17:45249430

ENST00000262233

664_701

636.3333333333334

816.0

Repeat

Note=WD 10

Hgene

EML1

chr14:100387214

chr17:45249430

ENST00000262233

709_768

636.3333333333334

816.0

Repeat

Note=WD 11

Hgene

EML1

chr14:100387214

chr17:45249430

ENST00000262233

775_814

636.3333333333334

816.0

Repeat

Note=WD 12

Hgene

EML1

chr14:100387214

chr17:45249430

ENST00000334192

664_701

655.3333333333334

835.0

Repeat

Note=WD 10

Hgene

EML1

chr14:100387214

chr17:45249430

ENST00000334192

709_768

655.3333333333334

835.0

Repeat

Note=WD 11

Hgene

EML1

chr14:100387214

chr17:45249430

ENST00000334192

775_814

655.3333333333334

835.0

Repeat

Note=WD 12

Tgene

CDC27

chr14:100387214

chr17:45249430

ENST00000066544

6_35

34.333333333333336

825.0

Repeat

Note=TPR 1

Tgene

CDC27

chr14:100387214

chr17:45249430

ENST00000531206

6_35

34.333333333333336

831.0

Repeat

Note=TPR 1

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Fusion Protein Structures

PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.

Fusion protein PDB link (fusion AA seq ID in FusionPDB)	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	AA seq	Len(AA seq)
PDB file >>>2060_EML1_100387214_CDC27_45249430_ranked_0.pdb	EML1	100387214	100387214	ENST00000531206	CDC27	chr17	45249430	-	MPPASPLGGGAAGRLTQGRPGLLSSASRRLDDSASAASSMEVTDRIASLEQRVQMQEDDIQLLKSALADVVRRLNITEEQQAVLNRKGPT KARPLMQTLPLRTTVNNGTVLPKKPTGSLPSPSGVRKETAVPATKRLNRSVSLLNACKLNRSTPSNIKRTSSSERVSPGGRRESNGDSRG NRNRTGSTSSSSSGKKNSESKPKEPVFSAEEGYVKMFLRGRPVTMYMPKDQVDSYSLEAKVELPTKRLKLEWVYGYRGRDCRNNLYLLPT GETVYFIASVVVLYNVEEQLQRHYAGHNDDVKCLAVHPDRITIATGQVAGTSKDGKQLPPHVRIWDSVTLNTLHVIGIGFFDRAVTCIAF SKSNGGTNLCAVDDSNDHVLSVWDWQKEEKLADVKCSNEAVFAADFHPTDTNIIVTCGKSHLYFWTLEGSSLNKKQGLFEKQEKPKFVLC VTFSENGDTITGDSSGNILVWGKGTNRISYAVQGAHEGGIFALCMLRDGTLVSGGGKDRKLISWSGNYQKLRKTEIPEQFGPIRTVAEGK GDVILIGTTRNFVLQGTLSGDFTPITQGHTDELWGLAIHASKSQFLTCGHDKHATLWDAVGHRPVWDKIIEDPAQSSGFHPSGSVVAVGT LTGRWFVFDTETKDLVTVHTDGNEQLSVMRYSPVHSEEALFLLATCYYRSGKAYKAYRLLKGHSCTTPQCKYLLAKCCVDLSKLAEGEQI LSGGVFNKQKSHDDIVTEFGDSACFTLSLLGHVYCKTDRLAKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKFTSLQNFSNCLP NSCTTQVPNHSLSHRQPETVLTETPQDTIELNRLNLESSNSKYSLNTDSSVSYIDSAVISPDTVPLGTGTSILSKQVQNKPKTGRSLLGG PAALSPLTPSFGILPLETPSPGDGSYLQNYTNTPPVIDVPSTGAPSKKTFRVLQSVARIGQTGTKSVFSQSGNSREVTPILAQTQSSGPQ TSTTPQVLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKGGITQPNINDSLEITKLDSSIISEGKISTI TPQIQAFNLQKAAAEGLMSLLREMGKGYLALCSYNCKEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEG MEIYSTTLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFR NAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFA NEKYKSALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNFSWAMDLDPKGANNQIKEAIDKRYLPDDEEPITQEEQIMGTDESQE	1459

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pLDDT score distribution

pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

EML1_pLDDT.png

CDC27_pLDDT.png

pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

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Ramachandran Plot of Fusion Protein Structure

Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.

Fusion AA seq ID in FusionPDB and their Ramachandran plots

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Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene	PPI interactors
EML1	ISG20L2, DCUN1D1, TUBA1B, TUBB3, TUBB2A, TUBB2B, DCTN1, Nek9, EML1, TUBA1A, FBXO28, EML4, TUBB, AGR2, KIAA1429, MTDH, MKRN2, FXR1, PTEN, UBE3A, PRPS2, NUDC, KXD1, NEK9, EML2,

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
EML1
CDC27

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

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Related Drugs to EML1-CDC27

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

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Related Diseases to EML1-CDC27

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner	Gene	Disease ID	Disease name	# pubmeds	Source
Hgene	EML1	C4284594	BAND HETEROTOPIA	2	GENOMICS_ENGLAND;UNIPROT
Hgene	EML1	C1848201	Subcortical Band Heterotopia	1	ORPHANET