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Center for Computational Systems Medicine level3
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Fusion Gene Summary

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Fusion Gene Sample Information

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Fusion ORF Analysis

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Fusion Amino Acid Sequences

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Fusion Protein Functional Features

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Fusion Protein Structure

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pLDDT scores

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Ramachandran Plot of Fusion Protein Structure

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Potential Active Site Information

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Potentially Interacting Small Molecules through Virtual Screening

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Biochemical Features of Small Molecules with ADME

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Drug Toxicity Information

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Fusion Protein-Protein Interaction

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Related drugs with this fusion protein

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Related disease with this fusion protein

Fusion Protein:EWSR1-SP3

Fusion Protein Summary

check button Fusion gene summary
Fusion partner gene informationFusion gene name: EWSR1-SP3
FusionPDB ID: 27857
FusionGDB2.0 ID: 27857
HgeneTgene
Gene symbol

EWSR1

SP3

Gene ID

2130

6670

Gene nameEWS RNA binding protein 1Sp3 transcription factor
SynonymsEWS|EWS-FLI1|bK984G1.4SPR2
Cytomap

22q12.2

2q31.1

Type of geneprotein-codingprotein-coding
DescriptionRNA-binding protein EWSEWS RNA-binding protein variant 6Ewing sarcoma breakpoint region 1Ewings sarcoma EWS-Fli1 (type 1) oncogenetranscription factor Sp3GC-binding transcription factor Sp3specificity protein 3
Modification date2020032920200313
UniProtAcc

Q01844

Q02447

Ensembl transtripts involved in fusion geneENST idsENST00000331029, ENST00000332035, 
ENST00000332050, ENST00000333395, 
ENST00000397938, ENST00000406548, 
ENST00000414183, 		ENST00000483084, 
ENST00000310015, ENST00000418194, 
ENST00000455789, 
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)* DoF score44 X 91 X 16=640646 X 5 X 4=120
# samples 986
** MAII scorelog2(98/64064*10)=-6.03058831983342
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(6/120*10)=-1
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)

PubMed: EWSR1 [Title/Abstract] AND SP3 [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)
Anticipated loss of major functional domain due to fusion event.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
TgeneSP3

GO:0006355

regulation of transcription, DNA-templated

12560508

TgeneSP3

GO:0045893

positive regulation of transcription, DNA-templated

12771217


check buttonFusion gene breakpoints across EWSR1 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check buttonFusion gene breakpoints across SP3 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.


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Fusion Gene Sample Information

check buttonFusion gene information from FusionGDB2.0.
check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerKB3..EWSR1chr22

29683123

+SP3chr2

174783513

-
ChimerKB3..EWSR1chr22

29687588

+SP3chr2

174783513

-


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Fusion ORF Analysis


check buttonFusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000332050EWSR1chr2229683123+ENST00000455789SP3chr2174783513-531011213281827499
ENST00000332050EWSR1chr2229683123+ENST00000310015SP3chr2174783513-531011213281827499
ENST00000332050EWSR1chr2229683123+ENST00000418194SP3chr2174783513-323911213281827499
ENST00000397938EWSR1chr2229683123+ENST00000455789SP3chr2174783513-530111123191818499
ENST00000397938EWSR1chr2229683123+ENST00000310015SP3chr2174783513-530111123191818499
ENST00000397938EWSR1chr2229683123+ENST00000418194SP3chr2174783513-323011123191818499
ENST00000406548EWSR1chr2229683123+ENST00000455789SP3chr2174783513-5046857641563499
ENST00000406548EWSR1chr2229683123+ENST00000310015SP3chr2174783513-5046857641563499
ENST00000406548EWSR1chr2229683123+ENST00000418194SP3chr2174783513-2975857641563499
ENST00000331029EWSR1chr2229683123+ENST00000455789SP3chr2174783513-5028839461545499
ENST00000331029EWSR1chr2229683123+ENST00000310015SP3chr2174783513-5028839461545499
ENST00000331029EWSR1chr2229683123+ENST00000418194SP3chr2174783513-2957839461545499
ENST00000414183EWSR1chr2229683123+ENST00000455789SP3chr2174783513-5021832211538505
ENST00000414183EWSR1chr2229683123+ENST00000310015SP3chr2174783513-5021832211538505
ENST00000414183EWSR1chr2229683123+ENST00000418194SP3chr2174783513-2950832211538505
ENST00000333395EWSR1chr2229683123+ENST00000455789SP3chr2174783513-5001812191518499
ENST00000333395EWSR1chr2229683123+ENST00000310015SP3chr2174783513-5001812191518499
ENST00000333395EWSR1chr2229683123+ENST00000418194SP3chr2174783513-2930812191518499
ENST00000332035EWSR1chr2229683123+ENST00000455789SP3chr2174783513-4825636111342443
ENST00000332035EWSR1chr2229683123+ENST00000310015SP3chr2174783513-4825636111342443
ENST00000332035EWSR1chr2229683123+ENST00000418194SP3chr2174783513-2754636111342443
ENST00000397938EWSR1chr2229687588+ENST00000455789SP3chr2174783513-552013313192037572
ENST00000397938EWSR1chr2229687588+ENST00000310015SP3chr2174783513-552013313192037572
ENST00000397938EWSR1chr2229687588+ENST00000418194SP3chr2174783513-344913313192037572
ENST00000406548EWSR1chr2229687588+ENST00000455789SP3chr2174783513-52621073641779571
ENST00000406548EWSR1chr2229687588+ENST00000310015SP3chr2174783513-52621073641779571
ENST00000406548EWSR1chr2229687588+ENST00000418194SP3chr2174783513-31911073641779571
ENST00000414183EWSR1chr2229687588+ENST00000455789SP3chr2174783513-52371048211754577
ENST00000414183EWSR1chr2229687588+ENST00000310015SP3chr2174783513-52371048211754577
ENST00000414183EWSR1chr2229687588+ENST00000418194SP3chr2174783513-31661048211754577
ENST00000332035EWSR1chr2229687588+ENST00000455789SP3chr2174783513-5044855111561516
ENST00000332035EWSR1chr2229687588+ENST00000310015SP3chr2174783513-5044855111561516
ENST00000332035EWSR1chr2229687588+ENST00000418194SP3chr2174783513-2973855111561516

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score

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Fusion Amino Acid Sequences


check button For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>27857_27857_1_EWSR1-SP3_EWSR1_chr22_29683123_ENST00000331029_SP3_chr2_174783513_ENST00000310015_length(amino acids)=499AA_BP=265
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQNIRIKE
EEPDPEEWQLSGDSTLNTNDLTHLRVQVVDEEGDQQHQEGKRLRRVACTCPNCKEGGGRGTNLGKKKQHICHIPGCGKVYGKTSHLRAHL
RWHSGERPFVCNWMYCGKRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTHQNKKGIHSSSTVLASVEAARDDTLITAGG

--------------------------------------------------------------

>27857_27857_2_EWSR1-SP3_EWSR1_chr22_29683123_ENST00000331029_SP3_chr2_174783513_ENST00000418194_length(amino acids)=499AA_BP=265
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQNIRIKE
EEPDPEEWQLSGDSTLNTNDLTHLRVQVVDEEGDQQHQEGKRLRRVACTCPNCKEGGGRGTNLGKKKQHICHIPGCGKVYGKTSHLRAHL
RWHSGERPFVCNWMYCGKRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTHQNKKGIHSSSTVLASVEAARDDTLITAGG

--------------------------------------------------------------

>27857_27857_3_EWSR1-SP3_EWSR1_chr22_29683123_ENST00000331029_SP3_chr2_174783513_ENST00000455789_length(amino acids)=499AA_BP=265
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQNIRIKE
EEPDPEEWQLSGDSTLNTNDLTHLRVQVVDEEGDQQHQEGKRLRRVACTCPNCKEGGGRGTNLGKKKQHICHIPGCGKVYGKTSHLRAHL
RWHSGERPFVCNWMYCGKRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTHQNKKGIHSSSTVLASVEAARDDTLITAGG

--------------------------------------------------------------

>27857_27857_4_EWSR1-SP3_EWSR1_chr22_29683123_ENST00000332035_SP3_chr2_174783513_ENST00000310015_length(amino acids)=443AA_BP=209
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQP
PTSYPPQTGSYSQAPSQYSQQSSSYGQQNIRIKEEEPDPEEWQLSGDSTLNTNDLTHLRVQVVDEEGDQQHQEGKRLRRVACTCPNCKEG
GGRGTNLGKKKQHICHIPGCGKVYGKTSHLRAHLRWHSGERPFVCNWMYCGKRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAK

--------------------------------------------------------------

>27857_27857_5_EWSR1-SP3_EWSR1_chr22_29683123_ENST00000332035_SP3_chr2_174783513_ENST00000418194_length(amino acids)=443AA_BP=209
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQP
PTSYPPQTGSYSQAPSQYSQQSSSYGQQNIRIKEEEPDPEEWQLSGDSTLNTNDLTHLRVQVVDEEGDQQHQEGKRLRRVACTCPNCKEG
GGRGTNLGKKKQHICHIPGCGKVYGKTSHLRAHLRWHSGERPFVCNWMYCGKRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAK

--------------------------------------------------------------

>27857_27857_6_EWSR1-SP3_EWSR1_chr22_29683123_ENST00000332035_SP3_chr2_174783513_ENST00000455789_length(amino acids)=443AA_BP=209
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQP
PTSYPPQTGSYSQAPSQYSQQSSSYGQQNIRIKEEEPDPEEWQLSGDSTLNTNDLTHLRVQVVDEEGDQQHQEGKRLRRVACTCPNCKEG
GGRGTNLGKKKQHICHIPGCGKVYGKTSHLRAHLRWHSGERPFVCNWMYCGKRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAK

--------------------------------------------------------------

>27857_27857_7_EWSR1-SP3_EWSR1_chr22_29683123_ENST00000332050_SP3_chr2_174783513_ENST00000310015_length(amino acids)=499AA_BP=265
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQNIRIKE
EEPDPEEWQLSGDSTLNTNDLTHLRVQVVDEEGDQQHQEGKRLRRVACTCPNCKEGGGRGTNLGKKKQHICHIPGCGKVYGKTSHLRAHL
RWHSGERPFVCNWMYCGKRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTHQNKKGIHSSSTVLASVEAARDDTLITAGG

--------------------------------------------------------------

>27857_27857_8_EWSR1-SP3_EWSR1_chr22_29683123_ENST00000332050_SP3_chr2_174783513_ENST00000418194_length(amino acids)=499AA_BP=265
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQNIRIKE
EEPDPEEWQLSGDSTLNTNDLTHLRVQVVDEEGDQQHQEGKRLRRVACTCPNCKEGGGRGTNLGKKKQHICHIPGCGKVYGKTSHLRAHL
RWHSGERPFVCNWMYCGKRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTHQNKKGIHSSSTVLASVEAARDDTLITAGG

--------------------------------------------------------------

>27857_27857_9_EWSR1-SP3_EWSR1_chr22_29683123_ENST00000332050_SP3_chr2_174783513_ENST00000455789_length(amino acids)=499AA_BP=265
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQNIRIKE
EEPDPEEWQLSGDSTLNTNDLTHLRVQVVDEEGDQQHQEGKRLRRVACTCPNCKEGGGRGTNLGKKKQHICHIPGCGKVYGKTSHLRAHL
RWHSGERPFVCNWMYCGKRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTHQNKKGIHSSSTVLASVEAARDDTLITAGG

--------------------------------------------------------------

>27857_27857_10_EWSR1-SP3_EWSR1_chr22_29683123_ENST00000333395_SP3_chr2_174783513_ENST00000310015_length(amino acids)=499AA_BP=265
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQNIRIKE
EEPDPEEWQLSGDSTLNTNDLTHLRVQVVDEEGDQQHQEGKRLRRVACTCPNCKEGGGRGTNLGKKKQHICHIPGCGKVYGKTSHLRAHL
RWHSGERPFVCNWMYCGKRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTHQNKKGIHSSSTVLASVEAARDDTLITAGG

--------------------------------------------------------------

>27857_27857_11_EWSR1-SP3_EWSR1_chr22_29683123_ENST00000333395_SP3_chr2_174783513_ENST00000418194_length(amino acids)=499AA_BP=265
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQNIRIKE
EEPDPEEWQLSGDSTLNTNDLTHLRVQVVDEEGDQQHQEGKRLRRVACTCPNCKEGGGRGTNLGKKKQHICHIPGCGKVYGKTSHLRAHL
RWHSGERPFVCNWMYCGKRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTHQNKKGIHSSSTVLASVEAARDDTLITAGG

--------------------------------------------------------------

>27857_27857_12_EWSR1-SP3_EWSR1_chr22_29683123_ENST00000333395_SP3_chr2_174783513_ENST00000455789_length(amino acids)=499AA_BP=265
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQNIRIKE
EEPDPEEWQLSGDSTLNTNDLTHLRVQVVDEEGDQQHQEGKRLRRVACTCPNCKEGGGRGTNLGKKKQHICHIPGCGKVYGKTSHLRAHL
RWHSGERPFVCNWMYCGKRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTHQNKKGIHSSSTVLASVEAARDDTLITAGG

--------------------------------------------------------------

>27857_27857_13_EWSR1-SP3_EWSR1_chr22_29683123_ENST00000397938_SP3_chr2_174783513_ENST00000310015_length(amino acids)=499AA_BP=265
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQNIRIKE
EEPDPEEWQLSGDSTLNTNDLTHLRVQVVDEEGDQQHQEGKRLRRVACTCPNCKEGGGRGTNLGKKKQHICHIPGCGKVYGKTSHLRAHL
RWHSGERPFVCNWMYCGKRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTHQNKKGIHSSSTVLASVEAARDDTLITAGG

--------------------------------------------------------------

>27857_27857_14_EWSR1-SP3_EWSR1_chr22_29683123_ENST00000397938_SP3_chr2_174783513_ENST00000418194_length(amino acids)=499AA_BP=265
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQNIRIKE
EEPDPEEWQLSGDSTLNTNDLTHLRVQVVDEEGDQQHQEGKRLRRVACTCPNCKEGGGRGTNLGKKKQHICHIPGCGKVYGKTSHLRAHL
RWHSGERPFVCNWMYCGKRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTHQNKKGIHSSSTVLASVEAARDDTLITAGG

--------------------------------------------------------------

>27857_27857_15_EWSR1-SP3_EWSR1_chr22_29683123_ENST00000397938_SP3_chr2_174783513_ENST00000455789_length(amino acids)=499AA_BP=265
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQNIRIKE
EEPDPEEWQLSGDSTLNTNDLTHLRVQVVDEEGDQQHQEGKRLRRVACTCPNCKEGGGRGTNLGKKKQHICHIPGCGKVYGKTSHLRAHL
RWHSGERPFVCNWMYCGKRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTHQNKKGIHSSSTVLASVEAARDDTLITAGG

--------------------------------------------------------------

>27857_27857_16_EWSR1-SP3_EWSR1_chr22_29683123_ENST00000406548_SP3_chr2_174783513_ENST00000310015_length(amino acids)=499AA_BP=265
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQNIRIKE
EEPDPEEWQLSGDSTLNTNDLTHLRVQVVDEEGDQQHQEGKRLRRVACTCPNCKEGGGRGTNLGKKKQHICHIPGCGKVYGKTSHLRAHL
RWHSGERPFVCNWMYCGKRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTHQNKKGIHSSSTVLASVEAARDDTLITAGG

--------------------------------------------------------------

>27857_27857_17_EWSR1-SP3_EWSR1_chr22_29683123_ENST00000406548_SP3_chr2_174783513_ENST00000418194_length(amino acids)=499AA_BP=265
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQNIRIKE
EEPDPEEWQLSGDSTLNTNDLTHLRVQVVDEEGDQQHQEGKRLRRVACTCPNCKEGGGRGTNLGKKKQHICHIPGCGKVYGKTSHLRAHL
RWHSGERPFVCNWMYCGKRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTHQNKKGIHSSSTVLASVEAARDDTLITAGG

--------------------------------------------------------------

>27857_27857_18_EWSR1-SP3_EWSR1_chr22_29683123_ENST00000406548_SP3_chr2_174783513_ENST00000455789_length(amino acids)=499AA_BP=265
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQNIRIKE
EEPDPEEWQLSGDSTLNTNDLTHLRVQVVDEEGDQQHQEGKRLRRVACTCPNCKEGGGRGTNLGKKKQHICHIPGCGKVYGKTSHLRAHL
RWHSGERPFVCNWMYCGKRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTHQNKKGIHSSSTVLASVEAARDDTLITAGG

--------------------------------------------------------------

>27857_27857_19_EWSR1-SP3_EWSR1_chr22_29683123_ENST00000414183_SP3_chr2_174783513_ENST00000310015_length(amino acids)=505AA_BP=271
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTVEGTSTGYTTPTAPQ
AYSQPVQGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQ
VPGSYPMQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQ
NIRIKEEEPDPEEWQLSGDSTLNTNDLTHLRVQVVDEEGDQQHQEGKRLRRVACTCPNCKEGGGRGTNLGKKKQHICHIPGCGKVYGKTS
HLRAHLRWHSGERPFVCNWMYCGKRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTHQNKKGIHSSSTVLASVEAARDDT

--------------------------------------------------------------

>27857_27857_20_EWSR1-SP3_EWSR1_chr22_29683123_ENST00000414183_SP3_chr2_174783513_ENST00000418194_length(amino acids)=505AA_BP=271
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTVEGTSTGYTTPTAPQ
AYSQPVQGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQ
VPGSYPMQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQ
NIRIKEEEPDPEEWQLSGDSTLNTNDLTHLRVQVVDEEGDQQHQEGKRLRRVACTCPNCKEGGGRGTNLGKKKQHICHIPGCGKVYGKTS
HLRAHLRWHSGERPFVCNWMYCGKRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTHQNKKGIHSSSTVLASVEAARDDT

--------------------------------------------------------------

>27857_27857_21_EWSR1-SP3_EWSR1_chr22_29683123_ENST00000414183_SP3_chr2_174783513_ENST00000455789_length(amino acids)=505AA_BP=271
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTVEGTSTGYTTPTAPQ
AYSQPVQGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQ
VPGSYPMQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQ
NIRIKEEEPDPEEWQLSGDSTLNTNDLTHLRVQVVDEEGDQQHQEGKRLRRVACTCPNCKEGGGRGTNLGKKKQHICHIPGCGKVYGKTS
HLRAHLRWHSGERPFVCNWMYCGKRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTHQNKKGIHSSSTVLASVEAARDDT

--------------------------------------------------------------

>27857_27857_22_EWSR1-SP3_EWSR1_chr22_29687588_ENST00000332035_SP3_chr2_174783513_ENST00000310015_length(amino acids)=516AA_BP=275
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQP
PTSYPPQTGSYSQAPSQYSQQSSSYGQQSSFRQDHPSSMGVYGQESGGFSGPGENRSMSGPDNRGRGRGGFDRGGMSRGGRGGGRGGMGS
AGERGGFNKPGDIRIKEEEPDPEEWQLSGDSTLNTNDLTHLRVQVVDEEGDQQHQEGKRLRRVACTCPNCKEGGGRGTNLGKKKQHICHI
PGCGKVYGKTSHLRAHLRWHSGERPFVCNWMYCGKRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTHQNKKGIHSSSTV

--------------------------------------------------------------

>27857_27857_23_EWSR1-SP3_EWSR1_chr22_29687588_ENST00000332035_SP3_chr2_174783513_ENST00000418194_length(amino acids)=516AA_BP=275
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQP
PTSYPPQTGSYSQAPSQYSQQSSSYGQQSSFRQDHPSSMGVYGQESGGFSGPGENRSMSGPDNRGRGRGGFDRGGMSRGGRGGGRGGMGS
AGERGGFNKPGDIRIKEEEPDPEEWQLSGDSTLNTNDLTHLRVQVVDEEGDQQHQEGKRLRRVACTCPNCKEGGGRGTNLGKKKQHICHI
PGCGKVYGKTSHLRAHLRWHSGERPFVCNWMYCGKRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTHQNKKGIHSSSTV

--------------------------------------------------------------

>27857_27857_24_EWSR1-SP3_EWSR1_chr22_29687588_ENST00000332035_SP3_chr2_174783513_ENST00000455789_length(amino acids)=516AA_BP=275
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQP
PTSYPPQTGSYSQAPSQYSQQSSSYGQQSSFRQDHPSSMGVYGQESGGFSGPGENRSMSGPDNRGRGRGGFDRGGMSRGGRGGGRGGMGS
AGERGGFNKPGDIRIKEEEPDPEEWQLSGDSTLNTNDLTHLRVQVVDEEGDQQHQEGKRLRRVACTCPNCKEGGGRGTNLGKKKQHICHI
PGCGKVYGKTSHLRAHLRWHSGERPFVCNWMYCGKRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTHQNKKGIHSSSTV

--------------------------------------------------------------

>27857_27857_25_EWSR1-SP3_EWSR1_chr22_29687588_ENST00000397938_SP3_chr2_174783513_ENST00000310015_length(amino acids)=572AA_BP=331
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSSFRQD
HPSSMGVYGQESGGFSGPGENRSMSGPDNRGRGRGGFDRGGMSRGGRGGGRGGMGSAGERGGFNKPGDIRIKEEEPDPEEWQLSGDSTLN
TNDLTHLRVQVVDEEGDQQHQEGKRLRRVACTCPNCKEGGGRGTNLGKKKQHICHIPGCGKVYGKTSHLRAHLRWHSGERPFVCNWMYCG
KRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTHQNKKGIHSSSTVLASVEAARDDTLITAGGTTLILANIQQGSVSGIG

--------------------------------------------------------------

>27857_27857_26_EWSR1-SP3_EWSR1_chr22_29687588_ENST00000397938_SP3_chr2_174783513_ENST00000418194_length(amino acids)=572AA_BP=331
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSSFRQD
HPSSMGVYGQESGGFSGPGENRSMSGPDNRGRGRGGFDRGGMSRGGRGGGRGGMGSAGERGGFNKPGDIRIKEEEPDPEEWQLSGDSTLN
TNDLTHLRVQVVDEEGDQQHQEGKRLRRVACTCPNCKEGGGRGTNLGKKKQHICHIPGCGKVYGKTSHLRAHLRWHSGERPFVCNWMYCG
KRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTHQNKKGIHSSSTVLASVEAARDDTLITAGGTTLILANIQQGSVSGIG

--------------------------------------------------------------

>27857_27857_27_EWSR1-SP3_EWSR1_chr22_29687588_ENST00000397938_SP3_chr2_174783513_ENST00000455789_length(amino acids)=572AA_BP=331
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSSFRQD
HPSSMGVYGQESGGFSGPGENRSMSGPDNRGRGRGGFDRGGMSRGGRGGGRGGMGSAGERGGFNKPGDIRIKEEEPDPEEWQLSGDSTLN
TNDLTHLRVQVVDEEGDQQHQEGKRLRRVACTCPNCKEGGGRGTNLGKKKQHICHIPGCGKVYGKTSHLRAHLRWHSGERPFVCNWMYCG
KRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTHQNKKGIHSSSTVLASVEAARDDTLITAGGTTLILANIQQGSVSGIG

--------------------------------------------------------------

>27857_27857_28_EWSR1-SP3_EWSR1_chr22_29687588_ENST00000406548_SP3_chr2_174783513_ENST00000310015_length(amino acids)=571AA_BP=330
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSSFRQD
HPSSMGVYGQESGGFSGPGENRSMSGPDNRGRGRGGFDRGGMSRGGRGGGRGGMGAGERGGFNKPGDIRIKEEEPDPEEWQLSGDSTLNT
NDLTHLRVQVVDEEGDQQHQEGKRLRRVACTCPNCKEGGGRGTNLGKKKQHICHIPGCGKVYGKTSHLRAHLRWHSGERPFVCNWMYCGK
RFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTHQNKKGIHSSSTVLASVEAARDDTLITAGGTTLILANIQQGSVSGIGT

--------------------------------------------------------------

>27857_27857_29_EWSR1-SP3_EWSR1_chr22_29687588_ENST00000406548_SP3_chr2_174783513_ENST00000418194_length(amino acids)=571AA_BP=330
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSSFRQD
HPSSMGVYGQESGGFSGPGENRSMSGPDNRGRGRGGFDRGGMSRGGRGGGRGGMGAGERGGFNKPGDIRIKEEEPDPEEWQLSGDSTLNT
NDLTHLRVQVVDEEGDQQHQEGKRLRRVACTCPNCKEGGGRGTNLGKKKQHICHIPGCGKVYGKTSHLRAHLRWHSGERPFVCNWMYCGK
RFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTHQNKKGIHSSSTVLASVEAARDDTLITAGGTTLILANIQQGSVSGIGT

--------------------------------------------------------------

>27857_27857_30_EWSR1-SP3_EWSR1_chr22_29687588_ENST00000406548_SP3_chr2_174783513_ENST00000455789_length(amino acids)=571AA_BP=330
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSSFRQD
HPSSMGVYGQESGGFSGPGENRSMSGPDNRGRGRGGFDRGGMSRGGRGGGRGGMGAGERGGFNKPGDIRIKEEEPDPEEWQLSGDSTLNT
NDLTHLRVQVVDEEGDQQHQEGKRLRRVACTCPNCKEGGGRGTNLGKKKQHICHIPGCGKVYGKTSHLRAHLRWHSGERPFVCNWMYCGK
RFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTHQNKKGIHSSSTVLASVEAARDDTLITAGGTTLILANIQQGSVSGIGT

--------------------------------------------------------------

>27857_27857_31_EWSR1-SP3_EWSR1_chr22_29687588_ENST00000414183_SP3_chr2_174783513_ENST00000310015_length(amino acids)=577AA_BP=336
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTVEGTSTGYTTPTAPQ
AYSQPVQGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQ
VPGSYPMQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQ
SSFRQDHPSSMGVYGQESGGFSGPGENRSMSGPDNRGRGRGGFDRGGMSRGGRGGGRGGMGAGERGGFNKPGDIRIKEEEPDPEEWQLSG
DSTLNTNDLTHLRVQVVDEEGDQQHQEGKRLRRVACTCPNCKEGGGRGTNLGKKKQHICHIPGCGKVYGKTSHLRAHLRWHSGERPFVCN
WMYCGKRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTHQNKKGIHSSSTVLASVEAARDDTLITAGGTTLILANIQQGS

--------------------------------------------------------------

>27857_27857_32_EWSR1-SP3_EWSR1_chr22_29687588_ENST00000414183_SP3_chr2_174783513_ENST00000418194_length(amino acids)=577AA_BP=336
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTVEGTSTGYTTPTAPQ
AYSQPVQGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQ
VPGSYPMQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQ
SSFRQDHPSSMGVYGQESGGFSGPGENRSMSGPDNRGRGRGGFDRGGMSRGGRGGGRGGMGAGERGGFNKPGDIRIKEEEPDPEEWQLSG
DSTLNTNDLTHLRVQVVDEEGDQQHQEGKRLRRVACTCPNCKEGGGRGTNLGKKKQHICHIPGCGKVYGKTSHLRAHLRWHSGERPFVCN
WMYCGKRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTHQNKKGIHSSSTVLASVEAARDDTLITAGGTTLILANIQQGS

--------------------------------------------------------------

>27857_27857_33_EWSR1-SP3_EWSR1_chr22_29687588_ENST00000414183_SP3_chr2_174783513_ENST00000455789_length(amino acids)=577AA_BP=336
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTVEGTSTGYTTPTAPQ
AYSQPVQGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQ
VPGSYPMQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQ
SSFRQDHPSSMGVYGQESGGFSGPGENRSMSGPDNRGRGRGGFDRGGMSRGGRGGGRGGMGAGERGGFNKPGDIRIKEEEPDPEEWQLSG
DSTLNTNDLTHLRVQVVDEEGDQQHQEGKRLRRVACTCPNCKEGGGRGTNLGKKKQHICHIPGCGKVYGKTSHLRAHLRWHSGERPFVCN
WMYCGKRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTHQNKKGIHSSSTVLASVEAARDDTLITAGGTTLILANIQQGS

--------------------------------------------------------------

Top

Fusion Protein Functional Features


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr22:/chr2:)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
EWSR1

Q01844

SP3

Q02447

FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.FUNCTION: Transcriptional factor that can act as an activator or repressor depending on isoform and/or post-translational modifications. Binds to GT and GC boxes promoter elements. Competes with SP1 for the GC-box promoters. Weak activator of transcription but can activate a number of genes involved in different processes such as cell-cycle regulation, hormone-induction and house-keeping. {ECO:0000269|PubMed:10391891, ECO:0000269|PubMed:11812829, ECO:0000269|PubMed:12419227, ECO:0000269|PubMed:12837748, ECO:0000269|PubMed:15247228, ECO:0000269|PubMed:15494207, ECO:0000269|PubMed:15554904, ECO:0000269|PubMed:16781829, ECO:0000269|PubMed:17548428, ECO:0000269|PubMed:18187045, ECO:0000269|PubMed:18617891, ECO:0000269|PubMed:9278495}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page

* Minus value of BPloci means that the break pointn is located before the CDS.
- Retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

- Not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note


Top

Fusion Protein Structures

check button PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.
Fusion protein PDB link (fusion AA seq ID in FusionPDB)HgeneHchrHbpHstrandTgeneTchrTbpTstrandAA seqLen(AA seq)
PDB file (206) >>>206.pdbFusion protein BP residue: 209
CIF file (206) >>>206.cif		EWSR1chr2229683123+SP3chr2174783513-
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDV
SYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPVQGYGTGAYDT
TTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTSYSSTQPTSYDQS
SYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQ
QSSSYGQQNIRIKEEEPDPEEWQLSGDSTLNTNDLTHLRVQVVDEEGDQQ
HQEGKRLRRVACTCPNCKEGGGRGTNLGKKKQHICHIPGCGKVYGKTSHL
RAHLRWHSGERPFVCNWMYCGKRFTRSDELQRHRRTHTGEKKFVCPECSK
RFMRSDHLAKHIKTHQNKKGIHSSSTVLASVEAARDDTLITAGGTTLILA
443
3D view using mol* of 206 (AA BP:209)
PDB file (299) >>>299.pdbFusion protein BP residue: 265
CIF file (299) >>>299.cif		EWSR1chr2229683123+SP3chr2174783513-
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDV
SYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPVQGYGTGAYDT
TTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQ
PQSSTGGYNQPSLGYGQSNYSYPQVPGSYPMQPVTAPPSYPPTSYSSTQP
TSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQA
PSQYSQQSSSYGQQNIRIKEEEPDPEEWQLSGDSTLNTNDLTHLRVQVVD
EEGDQQHQEGKRLRRVACTCPNCKEGGGRGTNLGKKKQHICHIPGCGKVY
GKTSHLRAHLRWHSGERPFVCNWMYCGKRFTRSDELQRHRRTHTGEKKFV
CPECSKRFMRSDHLAKHIKTHQNKKGIHSSSTVLASVEAARDDTLITAGG
499
3D view using mol* of 299 (AA BP:265)
PDB file (313) >>>313.pdbFusion protein BP residue: 271
CIF file (313) >>>313.cif		EWSR1chr2229683123+SP3chr2174783513-
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDV
SYTQAQTTATYGQTAYATSYGQPPTVEGTSTGYTTPTAPQAYSQPVQGYG
TGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNK
PTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYPMQPVTAPPSYPPTS
YSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQT
GSYSQAPSQYSQQSSSYGQQNIRIKEEEPDPEEWQLSGDSTLNTNDLTHL
RVQVVDEEGDQQHQEGKRLRRVACTCPNCKEGGGRGTNLGKKKQHICHIP
GCGKVYGKTSHLRAHLRWHSGERPFVCNWMYCGKRFTRSDELQRHRRTHT
GEKKFVCPECSKRFMRSDHLAKHIKTHQNKKGIHSSSTVLASVEAARDDT
LITAGGTTLILANIQQGSVSGIGTVNTSATSNQDILTNTEIPLQLVTVSG
505
3D view using mol* of 313 (AA BP:271)
PDB file (329) >>>329.pdbFusion protein BP residue: 275
CIF file (329) >>>329.cif		EWSR1chr2229687588+SP3chr2174783513-
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDV
SYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPVQGYGTGAYDT
TTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTSYSSTQPTSYDQS
SYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQ
QSSSYGQQSSFRQDHPSSMGVYGQESGGFSGPGENRSMSGPDNRGRGRGG
FDRGGMSRGGRGGGRGGMGSAGERGGFNKPGDIRIKEEEPDPEEWQLSGD
STLNTNDLTHLRVQVVDEEGDQQHQEGKRLRRVACTCPNCKEGGGRGTNL
GKKKQHICHIPGCGKVYGKTSHLRAHLRWHSGERPFVCNWMYCGKRFTRS
DELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTHQNKKGIHSSSTV
LASVEAARDDTLITAGGTTLILANIQQGSVSGIGTVNTSATSNQDILTNT
516
3D view using mol* of 329 (AA BP:275)
PDB file (427) >>>427.pdbFusion protein BP residue: 330
CIF file (427) >>>427.cif		EWSR1chr2229687588+SP3chr2174783513-
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDV
SYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPVQGYGTGAYDT
TTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQ
PQSSTGGYNQPSLGYGQSNYSYPQVPGSYPMQPVTAPPSYPPTSYSSTQP
TSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQA
PSQYSQQSSSYGQQSSFRQDHPSSMGVYGQESGGFSGPGENRSMSGPDNR
GRGRGGFDRGGMSRGGRGGGRGGMGAGERGGFNKPGDIRIKEEEPDPEEW
QLSGDSTLNTNDLTHLRVQVVDEEGDQQHQEGKRLRRVACTCPNCKEGGG
RGTNLGKKKQHICHIPGCGKVYGKTSHLRAHLRWHSGERPFVCNWMYCGK
RFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTHQNKKGIH
SSSTVLASVEAARDDTLITAGGTTLILANIQQGSVSGIGTVNTSATSNQD
571
3D view using mol* of 427 (AA BP:330)
PDB file (429) >>>429.pdbFusion protein BP residue: 331
CIF file (429) >>>429.cif		EWSR1chr2229687588+SP3chr2174783513-
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDV
SYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPVQGYGTGAYDT
TTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQ
PQSSTGGYNQPSLGYGQSNYSYPQVPGSYPMQPVTAPPSYPPTSYSSTQP
TSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQA
PSQYSQQSSSYGQQSSFRQDHPSSMGVYGQESGGFSGPGENRSMSGPDNR
GRGRGGFDRGGMSRGGRGGGRGGMGSAGERGGFNKPGDIRIKEEEPDPEE
WQLSGDSTLNTNDLTHLRVQVVDEEGDQQHQEGKRLRRVACTCPNCKEGG
GRGTNLGKKKQHICHIPGCGKVYGKTSHLRAHLRWHSGERPFVCNWMYCG
KRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTHQNKKGI
HSSSTVLASVEAARDDTLITAGGTTLILANIQQGSVSGIGTVNTSATSNQ
572
3D view using mol* of 429 (AA BP:331)
PDB file (440) >>>440.pdbFusion protein BP residue: 336
CIF file (440) >>>440.cif		EWSR1chr2229687588+SP3chr2174783513-
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDV
SYTQAQTTATYGQTAYATSYGQPPTVEGTSTGYTTPTAPQAYSQPVQGYG
TGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNK
PTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYPMQPVTAPPSYPPTS
YSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQT
GSYSQAPSQYSQQSSSYGQQSSFRQDHPSSMGVYGQESGGFSGPGENRSM
SGPDNRGRGRGGFDRGGMSRGGRGGGRGGMGAGERGGFNKPGDIRIKEEE
PDPEEWQLSGDSTLNTNDLTHLRVQVVDEEGDQQHQEGKRLRRVACTCPN
CKEGGGRGTNLGKKKQHICHIPGCGKVYGKTSHLRAHLRWHSGERPFVCN
WMYCGKRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTHQ
NKKGIHSSSTVLASVEAARDDTLITAGGTTLILANIQQGSVSGIGTVNTS
577
3D view using mol* of 440 (AA BP:336)


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pLDDT score distribution

check button pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.
EWSR1_pLDDT.png
all structure
all structure
SP3_pLDDT.png
all structure
all structure

check button pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.
EWSR1_SP3_206_PAE.png (AA BP:209)
all structure
EWSR1_SP3_206_pLDDT.png (AA BP:209)
all structure
EWSR1_SP3_206_pLDDT_and_active_sites.png (AA BP:209)
all structure
EWSR1_SP3_206_violinplot.png (AA BP:209)
all structure
EWSR1_SP3_299_pLDDT.png (AA BP:265)
all structure
EWSR1_SP3_299_pLDDT_and_active_sites.png (AA BP:265)
all structure
EWSR1_SP3_299_violinplot.png (AA BP:265)
all structure
EWSR1_SP3_313_pLDDT.png (AA BP:271)
all structure
EWSR1_SP3_313_pLDDT_and_active_sites.png (AA BP:271)
all structure
EWSR1_SP3_313_violinplot.png (AA BP:271)
all structure
EWSR1_SP3_329_pLDDT.png (AA BP:275)
all structure
EWSR1_SP3_329_pLDDT_and_active_sites.png (AA BP:275)
all structure
EWSR1_SP3_329_violinplot.png (AA BP:275)
all structure
EWSR1_SP3_427_pLDDT.png (AA BP:330)
all structure
EWSR1_SP3_427_pLDDT_and_active_sites.png (AA BP:330)
all structure
EWSR1_SP3_427_violinplot.png (AA BP:330)
all structure
EWSR1_SP3_429_pLDDT.png (AA BP:331)
all structure
EWSR1_SP3_429_pLDDT_and_active_sites.png (AA BP:331)
all structure
EWSR1_SP3_429_violinplot.png (AA BP:331)
all structure
EWSR1_SP3_440_pLDDT.png (AA BP:336)
all structure
EWSR1_SP3_440_pLDDT_and_active_sites.png (AA BP:336)
all structure
EWSR1_SP3_440_violinplot.png (AA BP:336)
all structure


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Ramachandran Plot of Fusion Protein Structure


check button Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.
Fusion AA seq ID in FusionPDB and their Ramachandran plots
EWSR1_SP3_206.png
all structure
EWSR1_SP3_299.png
all structure
EWSR1_SP3_313.png
all structure
EWSR1_SP3_329.png
all structure
EWSR1_SP3_427.png
all structure
EWSR1_SP3_429.png
all structure
EWSR1_SP3_440.png
all structure

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Potential Active Site Information


check button The potential binding sites of these fusion proteins were identified using SiteMap, a module of the Schrodinger suite.
Fusion AA seq ID in FusionPDBSite scoreSizeD scoreVolumeExposureEnclosureContactPhobicPhilicBalanceDon/AccResidues
2061.012931.047319.6760.5830.7050.9470.8280.8680.9541.542Chain A: 377,381,384,385,387,388,389,390,397,398,3
99,400,401,402,407,408,409,410,411,413
2990.738410.62793.6390.5820.7011.020.3861.2140.3180.713Chain A: 320,321,322,337,338,339,340,341,342,356,3
60
3130.942840.984264.110.6410.6250.8970.7430.7740.9590.675Chain A: 446,447,448,449,450,452,459,460,461,462,4
63,464,469,470,471,472,473,475,493,495,496,497,498

3290.868810.873278.5160.7350.6090.7590.2151.0380.2070.354Chain A: 329,330,331,332,333,334,335,336,337,338,3
39,340,355,356,357,358,359,360,370,371,372,373,374
,375,377,378,385,386,399,400,401
4270.9351561.05795.6970.7870.420.3430.1720.4140.4150.545Chain A: 420,422,427,430,431,434,437,439,452,453,4
54,456,457,460
4290.754520.73689.5230.50.6070.8910.5990.9390.6370.68Chain A: 513,514,515,516,517,518,519,526,527,528,5
29,530
4400.865670.891170.1280.5960.6140.8630.7260.7580.9581.033Chain A: 511,514,515,518,519,520,521,522,523,524,5
31,532,533,534,535

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Potentially Interacting Small Molecules through Virtual Screening


check button The FDA-approved small molecule library molecules were subjected to virtual screening using the Glide.
Fusion AA seq ID in FusionPDBZINC IDDrugBank IDDrug nameDocking scoreGlide gscore
427ZINC000000388081DB05381Histamine-4.47654-5.80814
427ZINC000049637509DB06636Isavuconazonium-4.90902-5.34102
427ZINC000001883067DB03651Picric acid-5.24503-5.24503
427ZINC000000034157DB06707Levonordefrin-5.19174-5.20924
427ZINC000000000850DB00744Zileuton-5.13991-5.15851
427ZINC000008551180DB00798Gentamicin-4.96483-5.14873
427ZINC000003803652DB00399Zoledronic acid-4.63588-5.14228
427ZINC000100296832DB09488Acrivastine-5.12555-5.13045
427ZINC000003806262DB00552Pentostatin-5.07359-5.10319
427ZINC000028108825DB06705Gadofosveset trisodium-3.70447-5.09607
427ZINC000084843283DB00356Chlorzoxazone-5.0606-5.0939
427ZINC000006827695DB04160Pyrophosphoric acid-4.85657-5.09007
427ZINC000000897085DB00358Mefloquine-5.08276-5.08526
427ZINC000001543475DB14126Tenofovir-4.45529-5.04119
427ZINC000058581064DB08930Dolutegravir-4.70837-4.99557
427ZINC000011677857DB06237Avanafil-4.95248-4.98938
427ZINC000000008492DB11145Oxyquinoline-4.97014-4.98814
427ZINC000000020259DB00852Pseudoephedrine-4.97442-4.98112
427ZINC000006382803DB00352Tioguanine-4.82213-4.97253
427ZINC000004474443DB01150Cefprozil-4.22045-4.95115

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check button Drug information from DrugBank of the top 20 interacting small molecules.
ZINC IDDrugBank IDDrug nameDrug typeSMILESDrug group
ZINC000000388081DB05381HistamineSmall moleculeNCCC1=CNC=N1Approved|Investigational
ZINC000049637509DB06636IsavuconazoniumSmall molecule[H]C(C)(OC(=O)N(C)C1=C(COC(=O)CNC)C=CC=N1)[N+]1=CN(C[C@](O)(C2=C(F)C=CC(F)=C2)[C@@]([H])(C)C2=NC(=CS2)C2=CC=C(C=C2)C#N)N=C1Approved|Investigational
ZINC000001883067DB03651Picric acidSmall moleculeOC1=C(C=C(C=C1[N+]([O-])=O)[N+]([O-])=O)[N+]([O-])=OExperimental
ZINC000000034157DB06707LevonordefrinSmall moleculeC[C@H](N)[C@H](O)C1=CC(O)=C(O)C=C1Approved
ZINC000003803652DB00399Zoledronic acidSmall moleculeOC(CN1C=CN=C1)(P(O)(O)=O)P(O)(O)=OApproved
ZINC000003806262DB00552PentostatinSmall moleculeOC[C@H]1O[C@H](C[C@@H]1O)N1C=NC2=C1N=CNC[C@H]2OApproved|Investigational
ZINC000084843283DB00356ChlorzoxazoneSmall moleculeClC1=CC2=C(OC(=O)N2)C=C1Approved
ZINC000006827695DB04160Pyrophosphoric acidSmall moleculeOP(O)(=O)OP(O)(O)=OApproved|Experimental
ZINC000001543475DB14126TenofovirSmall moleculeC[C@H](CN1C=NC2=C1N=CN=C2N)OCP(O)(O)=OExperimental|Investigational
ZINC000058581064DB08930DolutegravirSmall molecule[H][C@]12CN3C=C(C(=O)NCC4=CC=C(F)C=C4F)C(=O)C(O)=C3C(=O)N1[C@H](C)CCO2Approved
ZINC000011677857DB06237AvanafilSmall moleculeCOC1=C(Cl)C=C(CNC2=C(C=NC(=N2)N2CCC[C@H]2CO)C(=O)NCC2=NC=CC=N2)C=C1Approved
ZINC000000008492DB11145OxyquinolineSmall moleculeOC1=CC=CC2=C1N=CC=C2Approved|Vet_approved
ZINC000000020259DB00852PseudoephedrineSmall moleculeCN[C@@H](C)[C@@H](O)C1=CC=CC=C1Approved
ZINC000006382803DB00352TioguanineSmall moleculeNC1=NC(=S)C2=C(N1)N=CN2Approved

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Biochemical Features of Small Molecules


check button ADME (Absorption, Distribution, Metabolism, and Excretion) of drugs using QikProp(v3.9)
ZINC IDmol_MWdipoleSASAFOSAFISAPISAWPSAvolumedonorHBaccptHBIPHuman Oral AbsorptionPercent Human Oral AbsorptionRule Of FiveRule Of Three
ZINC000000388081111.1464.788315.23192.229118.535104.4680466.77632.59.295265.25300
ZINC000000388081111.1465.535313.53290.818116.688106.0260465.559339.188264.91700
ZINC000000388081111.1464.794316.56991.628116.549108.3930470.46832.59.292265.82300
ZINC000001883067229.1062.688387.4880314.26473.2240613.46513.7511.517242.26301
ZINC000000034157183.2071.584399.847106.772186.848106.2270646.19954.28.948252.10900
ZINC000000034157183.2072.284398.836105.841186.106106.8890643.15454.28.781252.1900
ZINC000000000850236.2885.297457.64285.783137.035198.52336.3755.01533.78.91375.35100
ZINC000000000850236.2885.297450.83785.532135.419201.89327.994746.83933.78.57376.55500
ZINC000008551180477.63.965721.155466.741254.414001439.4131116.959.0461022
ZINC000008551180477.67.622744.998510.764234.234001455.6281116.959.0461022
ZINC000008551180477.64.362682.464433.504248.96001389.6671116.958.8791022
ZINC000008551180477.63.649705.855457.863247.992001411.3081116.959.0621022
ZINC000008551180477.61.441703.707448.376255.331001410.2811116.959.0111022
ZINC000003803652272.0916.149414.00425.436272.399109.4776.692698.61218.759.817123.48901
ZINC000003803652272.09110.346412.62622.493273.968109.2846.881697.13318.758.891123.12401
ZINC000003803652272.0912.82416.54522.709279.09108.4066.34697.54618.759.904121.99201
ZINC000003803652272.09111.882412.71124.614274.426108.4675.205696.7418.759.026122.96801
ZINC000003803652272.09112.418410.05122.071274.507110.8272.646702.50318.758.712123.16601
ZINC000003803652272.09112.445414.59323.463272.373114.5554.201705.32118.758.718123.72201
ZINC000100296832348.4443.613654.428324.89778.794250.73701183.627158.906378.16700
ZINC000003806262268.2721.452502.362225.071237.12940.1620835.80349.88.337251.84800
ZINC000003806262268.2721.378469.669223.737214.90231.030809.61749.88.34256.16400
ZINC000028108825737.6969.678994.117223.532449.46319.6451.4792004.2186219.5011032
ZINC000028108825737.6962.2981041.332267.666452.611319.7841.2712036.9586219.0721032
ZINC000028108825737.69611.17929.353238.432413.58273.1054.2361934.9836219.1561032
ZINC000084843283169.5674.063322.7980106.536144.74471.517497.554139.228386.76600
ZINC000084843283169.5674.554322.4890105.966144.97771.546495.802139.258386.85700
ZINC000006827695177.9756.525306.4420295.843010.598452.0560610.264112.58601
ZINC000006827695177.9757.967307.0330297.7909.242451.967069.969112.14301
ZINC000006827695177.97513.968307.1260292.724014.402453.5260610.429113.38301
ZINC000000897085378.3177.557589.939192.62654.6122.313220.4011034.64724.29.439310000
ZINC000001543475287.2148.348504.272135.683258.29105.9154.383848.06410.78.314131.42801
ZINC000001543475287.2148.376485.449129.583251.84599.7254.296831.981410.78.324132.55201
ZINC000058581064419.38410.752671.499281.937165.698150.48573.3791193.65208.458.755383.67200
ZINC000058581064419.38410.808670.317276.157165.522158.3170.3281193.76108.458.94383.69800
ZINC000011677857483.9565.046784.956356.119102.499263.56462.7741435.48828.958.396310002
ZINC000011677857483.9562.464779.178356.47798.017255.369.3841436.6128.958.366310002
ZINC000011677857483.9566.413826.884353.3103.68298.69871.2061481.93228.958.32110002
ZINC000000008492145.162.589340.545068.394272.150527.27511.758.638310000
ZINC000000008492145.162.225339.564068.018271.5460525.50411.758.457310000
ZINC000000020259165.2351.645408.608167.77458.787182.0460662.69723.29.158384.99100
ZINC000000020259165.2351.679405.001165.63758.444180.9210657.17623.29.01384.84600
ZINC000006382803167.1882.913334.0990174.67282.56276.865508.157458.609368.38600
ZINC000006382803167.18810.353334.2370175.32682.48776.423508.122458.464368.2800
ZINC000006382803167.1883.972333.7640164.06191.94377.76507.0323.848.378369.61600
ZINC000004474443389.4255.794646.882196.806278.227152.33819.5111147.6474.2589.006117.18101
ZINC000004474443389.4258.143664.86196.035273.731163.8431.2551174.6394.2588.985119.44301


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Drug Toxicity Information


check button Toxicity information of individual drugs using eToxPred
ZINC IDSmileSurface AccessibilityToxicity
ZINC000000388081NCCc1cnc[nH]10.2129447840.352067312
ZINC000049637509CNCC(=O)OCc1cccnc1N(C)C(=O)O[C@@H](C)[n+]1cnn(C[C@](O)(c2cc(F)ccc2F)[C@@H](C)c2nc(-c3ccc(C#N)cc3)cs2)c10.0254160270.235213823
ZINC000001883067O=[N+]([O-])c1cc([N+](=O)[O-])c(O)c([N+](=O)[O-])c10.2714260510.71139895
ZINC000000034157C[C@H](N)[C@H](O)c1ccc(O)c(O)c10.1350791920.302260037
ZINC000000000850C[C@@H](c1cc2ccccc2s1)N(O)C(N)=O0.1363358820.223511692
ZINC000008551180CN[C@H]1[C@H](O)[C@H](O[C@@H]2[C@H](N)C[C@H](N)[C@@H](O[C@H]3O[C@@H]([C@@H](C)NC)CC[C@H]3N)[C@H]2O)OC[C@@]1(C)O0.0132652410.371016745
ZINC000003803652O=P(O)(O)C(O)(Cn1ccnc1)P(=O)(O)O0.1000522680.353941778
ZINC000100296832Cc1ccc(/C(=C/CN2CCCC2)c2cccc(/C=CC(=O)O)n2)cc10.222375690.247350052
ZINC000003806262OC[C@H]1O[C@@H](n2cnc3c2N=CNC[C@H]3O)C[C@@H]1O0.0242366470.504660299
ZINC000028108825O=C(O)CN(CCN(CC(=O)O)C[C@H](CO[P@](=O)(O)OC1CCC(c2ccccc2)(c2ccccc2)CC1)N(CC(=O)O)CC(=O)O)CC(=O)O0.0431071060.443371273
ZINC000084843283O=c1[nH]c2cc(Cl)ccc2o10.3045104230.412888168
ZINC000006827695O=P(O)(O)OP(=O)(O)O0.1281427470.580031414
ZINC000000897085O[C@@H](c1cc(C(F)(F)F)nc2c(C(F)(F)F)cccc12)[C@H]1CCCCN10.0827248270.290697747
ZINC000001543475C[C@H](Cn1cnc2c(N)ncnc21)OCP(=O)(O)O0.0923712960.318784803
ZINC000058581064C[C@@H]1CCO[C@H]2Cn3cc(C(=O)NCc4ccc(F)cc4F)c(=O)c(O)c3C(=O)N210.072657050.3133548
ZINC000011677857COc1ccc(CNc2nc(N3CCC[C@H]3CO)ncc2/C(O)=N/Cc2ncccn2)cc1Cl0.0878112790.167931707
ZINC000000008492Oc1cccc2cccnc120.4696341470.557043533
ZINC000000020259CN[C@@H](C)[C@@H](O)c1ccccc10.2085878950.312560893
ZINC000006382803Nc1nc2nc[nH]c2c(=S)[nH]10.100054570.571375233
ZINC000004474443C/C=CC1=C(C(=O)O)N2C(=O)[C@@H](NC(=O)[C@H](N)c3ccc(O)cc3)[C@H]2SC10.0675634840.161076886


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Fusion Protein-Protein Interaction


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)
GenePPI interactors
EWSR1PRTFDC1, ZDHHC3, MSC, SLC22A24, MYO1F, KXD1, KHDRBS2, DNAJB3, FASN, NLE1, MNS1, PRUNE2, WWP2, NDUFB1, BNIP3L, NSUN4, KRR1, WWP1, RMND5B, SLC1A1, RASL11B, DFFA, WDR37, RPS15A, CPSF6, C11orf16, YY1AP1, RNF183, MTCP1, TULP2, RBPMS, KEL, MYOZ2, FAM131C, HMGA1, NPPB, HERPUD1, CD177, RPL31, VPS72, ACTL6A, RAD23A, MAGEA11, CFDP1, DMRTB1, CXADR, ZNF165, SSBP2, TPGS2, RAB37, CETP, NDUFV1, DYNLL2, NBPF3, CEACAM5, GPBP1L1, SERP2, GNPDA1, C19orf57, ELAVL3, ELAVL4, LILRA3, BAD, CCDC7, MRPS18B, CUEDC2, CNST, TSPAN3, CCDC91, TRIM37, NINL, NTNG2, CPSF7, PGLS, EPT1, MYL6, SMAD4, TMSB4Y, TRPV5, MVK, MAPK1IP1L, MDFI, MTMR9, PLSCR1, RALYL, PDHX, C10orf12, RHOXF2, MATK, SALL2, AGT, KCNMB1, SUV39H2, SMNDC1, ARHGDIA, PUF60, GSK3B, ILK, CD2BP2, BARD1, CREBBP, BTK, SF1, SNRPC, ELK1, PTK2B, CALM1, POU4F1, EZH2, IRF3, TONSL, RFX3, HLTF, SUPT4H1, ZNF184, HIST1H2BN, BLZF1, HDAC3, FXR2, HMGN4, POLR3A, ECD, ZBTB1, SCMH1, SUZ12, E2F8, TRIM5, ZNF383, DHX9, SMN1, PCM1, RAD21, NDRG1, CEBPA, ELAVL1, SIRT7, HNRNPA1, TSG101, TP53, POLR2A, YBX1, TDRD3, CUL3, CUL4A, CUL4B, CUL5, CUL2, CUL1, COPS5, COPS6, DCUN1D1, CAND1, NEDD8, KCND3, ATN1, ATXN3, ERCC5, HBP1, HSPA2, PCBP1, USP7, SRSF5, TMEM126A, SAP30BP, GORASP2, MBD3, MRPS9, HAX1, SFXN1, ITGA5, TCIRG1, RNF168, GEMIN5, HAS1, MTCH1, NDUFA5, MCAT, MRPL57, HDAC2, ESR1, FN1, VCAM1, ITGA4, CD81, PRMT1, SF3B4, EP300, PRRC2A, EWSR1, FUS, ITGB5, NONO, TRAF3, HNRNPUL1, EPAS1, CHERP, CDK12, ITCH, WBP4, rev, RPA3, RPA2, RPA1, HSPA5, RIOK2, TRAF1, TRAF2, SEC24D, TFG, SEC24A, SSBP3, PRR13, ATPAF2, PEF1, JUN, CUL7, OBSL1, CCDC8, RNF2, BMI1, EGFR, ABL1, SRPK1, ABCE1, PRMT8, RPS6KB2, ACAA2, ACAT1, EIF4H, ANXA2, HIP1R, PICALM, POR, NTRK1, SCARNA22, NPM1, KRT2, PRDX2, S100A9, YWHAZ, DDX17, PCLO, ANXA6, SEC24C, GIPC1, CSRP2, FHL1, HNRNPA3, CTTN, MARCKSL1, PARP1, SERPINB3, CCDC50, KIF2A, KRT6B, HIST1H1D, STK38, H2AFV, PACSIN2, U2AF1, RSL24D1, XRCC5, ESYT3, RPL29, SDF2L1, LRP1B, MSN, SEC23A, RPL7A, SNX18, PPIL1, RPS27A, IGHM, SUMO3, GTF2I, RUVBL1, KRT16, RBM8A, RPL8, SRSF9, ZC3HAV1L, GAPDH, ETV3, IGF2R, COL5A2, HNRNPD, ANP32B, WAC, TFAP2A, TTN, CBR3, ARGLU1, HNRNPAB, SRRT, ATP1B3, COPS7B, PRR12, ATF3, NOMO3, NOMO1, NOMO2, EYA3, C1orf198, MAZ, U2AF2, SSB, TRA2B, C1orf52, HMGB1, HMGB1P10, RPRD1A, HNRNPH3, EIF5A2, C1orf131, SEC13, MAPRE1, CSTF2T, SRSF3, LENG1, UPF1, HDGFRP2, RPS10P7, RPS10P11, RPS10, RPS10P13, RPS10P4, RPS10P22, DACH1, ANO1, NCOR1, MLX, SUMO1P3, SUMO1, WIZ, PFDN6, ARFIP2, ZHX3, EEF1B2, MBIP, BAG6, DENND2A, PRCC, SRSF1, EIF4ENIF1, SPTAN1, CDCA8, PLS3, API5P1, API5, PSMA4, DNTTIP1, AKAP8, NCOA3, SMTN, FBRS, SMARCE1, ERC1, WBP11, SPTBN1, NFRKB, OLA1, ZNF207, R3HDM1, TRIM33, SAFB, UBFD1, SRSF7, SRSF2, GATA6, VDAC1P1, VDAC1, IL16, GMNN, ILF2, MED4, QKI, VCL, MFAP1, SNAP29, PADI1, BCL9, BCL9L, PKM, GPATCH11, CASC3, PSMC6, CACYBP, RPL12P6, RPL12P32, RPL12P14, RPL12, RPL12P2, RPL12P35, RPL12P19, TPI1, TPI1P1, CHAF1A, MIA3, CIC, SDCBP, CA2, FKBP3, ACE, NKX2-5, CSTF2, PFDN2, UBTF, FAM207A, LOC729774, BRD8, C12orf45, C1orf35, TCF20, SOD1, SPAG7, MED8, ETS2, ALDOC, FKBP4, INCENP, CEP85, CECR2, TFE3, SUPT16HP1, SUPT16H, MAPT, HTATSF1, RPS18P12, RPS18P5, KPNA2, TMX1, CKAP4, HSPE1, COPRS, PTGES3, LAMP2, ERLIN2, CTNNBL1, TOMM22, NRBF2, C9orf78, NCOA6, MED26, RANBP1, LOC389842, LOC727803, HMGB3, CANX, PUS7, RPSAP19, RPSA, RPSAP18, RPSAP58, RPSAP15, RPSAP8, RPSAP9, RPSAP12, RPSAP29, RPSAP61, ARHGAP17, USF1, PSIP1, SNRPEP2, SNRPE, NUDT5, PPM1G, OTUB1, AHCY, COPS3, NSMCE2, SAE1, PROSER1, GRWD1, CREB5, TAF9B, RBM33, EDF1, PGK1, FAM114A2, SRRM1, RAD23B, CIAPIN1, CIAPIN1P, LRRC59, PABPN1, KMT2A, RPRD1B, GPATCH8, CCDC43, DGCR14, PPP1R2, ERICH1, EIF5A, EIF5AL1, BAG3, PCNA, SOX7, PNISR, FAM168A, MED15, SRSF11, SIRT1, RSF1, MAML1, HPRT1, SPDL1, CRTC3, CEP55, CDV3, ALYREF, RNF40, STOML2, DGCR8, NUCKS1, UBN2, PSMD7, WNT10A, HMBS, KHDRBS1, VBP1, NCSTN, CDCA2, SFSWAP, ZRANB2, DDB1, RBBP6, ZEB1, SRSF6, LOC644422, EIF2S1, RFX5, RPS19, RPS19P3, TALDO1, CWC15, CDCA5, LOC645086, C11orf58, TXN, STX12, PHRF1, BSG, TAF4, SH3GL1, LIN37, HRNR, FAM192A, RRBP1, KIAA0907, GOLGB1, PAX9, P4HB, CHMP5, LDHB, CALR, SUMO2P1, SUMO2, PDIA6, AHSA1, EN2, CCDC124, RPLP0P6, RPLP0P2, RPLP0P3, RPLP0, NIPBL, PDLIM4, PRKCSH, C15orf39, HNRNPDL, PDIA4, NUP210, RPLP2P3, RPLP2, PRDX4, DAZAP1, UBE2T, PHAX, AMOT, MARCKS, LOC284685, SMARCC1, BCORL1, RFC4, GLRX3, ANP32E, HYOU1, NPM3, ATF7IP, SARNP, TRA2A, HDGF, STIP1, PELP1, KCTD12, GLO1, PCF11, CLIC1, DNAJC8, RNF114, SLC4A1AP, FAM50A, GTF2A1, PRPF40A, CDC37, PPIAP22, PPIA, SMARCC2, MEGF11, KIAA1143, DENR, LAMP1, MYBL2, PITX1, UBE2MP1, UBE2M, CHTF8, OTX1, NACA, FNBP4, GTF2F2, GLTSCR1, GTF2E1, PQBP1, EMD, RNF113A, GPALPP1, SNRPA, RRP15, RPS25, RPS25P8, GMEB2, LNPEP, DNAJB1, IGBP1P1, IGBP1, HINT1, ARID1A, PPIB, ANXA11, MATR3, Sgol2, PPARGC1A, MCM2, Ksr1, UBASH3B, SFPQ, CAPN13, HEY1, BRCA1, MTCH2, PPIE, TBX3, BMP4, CTNNB1, GSK3A, HNF1B, TCF7L2, TRIP4, YAF2, ZNF217, AAR2, PIH1D1, EFTUD2, TNIP2, CHD3, CHD4, HEXIM1, MEPCE, LARP7, RUNX1, AGR2, RECQL4, REST, CDK9, SMARCA4, DDIT3, FLI1, TP53BP1, MDC1, METTL3, METTL14, KIAA1429, RC3H1, RC3H2, ATG16L1, PHB, DISC1, NR2C2, UBQLN2, ZFYVE21, XRCC6, AGRN, USP19, HIST1H4A, APEX1, DDX5, SNRNP70, SNRPB, SNRPD1, SNRPD2, SNRPD3, RNU1-1, RBMX, HNRNPM, HNRNPA2B1, TAF15, DDX3X, TARDBP, CLINT1, HNRNPL, NUMA1, ZFR, SNRNP200, ZNF326, HNRNPK, SF3B1, TOE1, HSPA8, SNRPB2, DDX20, GOT2, ILF3, PRPF6, ZNF638, HNRNPF, HNRNPH1, HNRNPR, VCP, CAD, CCAR2, DDX23, GEMIN4, HSPA1A, PCMTD1, POTEF, PRMT5, RBM45, SAFB2, SF3B2, SF3B3, SNRPF, SRSF4, THRAP3, TIA1, TTC7A, ZCCHC8, ITFG1, ARAF, BRD7, SOX2, ARIH2, PLEKHA4, NGB, OPTN, ZC3H18, CELF1, MKI67, INS, Apc2, FBP1, N, ZNF768, SYNCRIP, KDM5C, DDX58, OGT, SPOP, UFL1, DDRGK1, WDR5, TPX2, MALL, SOX21, POU3F3, PTP4A3, TRIM8, RCHY1, nsp14, SOX5,


check button Protein-protein interactors based on sequence similarity (STRING)
GeneSTRING network
EWSR1all structure
SP3all structure


check button - Retained interactions in fusion protein (protein functional feature from UniProt).
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost interactions due to fusion (protein functional feature from UniProt).
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs to EWSR1-SP3


check button Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDrugSourcePMID

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Related Diseases to EWSR1-SP3


check button Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDiseaseSourcePMID
EWSR1SP3AstrocytomaMyCancerGenome
EWSR1SP3Breast Invasive Ductal CarcinomaMyCancerGenome
EWSR1SP3Clear Cell Renal Cell CarcinomaMyCancerGenome
EWSR1SP3Dermatofibrosarcoma ProtuberansMyCancerGenome
EWSR1SP3Endometrial Mixed AdenocarcinomaMyCancerGenome

check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource
HgeneEWSR1C0553580Ewings sarcoma3CTD_human;ORPHANET
HgeneEWSR1C0002736Amyotrophic Lateral Sclerosis1GENOMICS_ENGLAND
HgeneEWSR1C0033578Prostatic Neoplasms1CTD_human
HgeneEWSR1C0206651Clear Cell Sarcoma of Soft Tissue1ORPHANET
HgeneEWSR1C0206663Neuroectodermal Tumor, Primitive1CTD_human
HgeneEWSR1C0279980Extra-osseous Ewing's sarcoma1ORPHANET
HgeneEWSR1C0334584Spongioblastoma1CTD_human
HgeneEWSR1C0334596Medulloepithelioma1CTD_human
HgeneEWSR1C0376358Malignant neoplasm of prostate1CTD_human
HgeneEWSR1C0700367Ependymoblastoma1CTD_human
HgeneEWSR1C0751675Cerebral Primitive Neuroectodermal Tumor1CTD_human
HgeneEWSR1C1275278Extraskeletal Myxoid Chondrosarcoma1ORPHANET