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	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein Structure
	pLDDT scores
	Ramachandran Plot of Fusion Protein Structure
	Potential Active Site Information
	Potentially Interacting Small Molecules through Virtual Screening
	Biochemical Features of Small Molecules with ADME
	Drug Toxicity Information
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:EWSR1-WT1

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: EWSR1-WT1
	FusionPDB ID: 27858
	FusionGDB2.0 ID: 27858
		Hgene	Tgene
	Gene symbol	EWSR1	WT1
	Gene ID	2130	7490
	Gene name	EWS RNA binding protein 1	WT1 transcription factor
	Synonyms	EWS\|EWS-FLI1\|bK984G1.4	AWT1\|GUD\|NPHS4\|WAGR\|WIT-2\|WT33
	Cytomap	22q12.2	11p13
	Type of gene	protein-coding	protein-coding
	Description	RNA-binding protein EWSEWS RNA-binding protein variant 6Ewing sarcoma breakpoint region 1Ewings sarcoma EWS-Fli1 (type 1) oncogene	Wilms tumor proteinWilms tumor 1
	Modification date	20200329	20200320
	UniProtAcc	Q01844	P19544
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000331029, ENST00000332035, ENST00000332050, ENST00000333395, ENST00000397938, ENST00000406548, ENST00000414183,	ENST00000332351, ENST00000379079, ENST00000448076, ENST00000530998,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	44 X 91 X 16=64064	3 X 6 X 5=90
	# samples	98	4
	** MAII score	log2(98/64064*10)=-6.03058831983342 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(4/90*10)=-1.16992500144231 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)	PubMed: EWSR1 [Title/Abstract] AND WT1 [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	EWSR1(29683128)-WT1(32414301), # samples:2
Anticipated loss of major functional domain due to fusion event.	EWSR1-WT1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. EWSR1-WT1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. EWSR1-WT1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. EWSR1-WT1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. EWSR1-WT1 seems lost the major protein functional domain in Hgene partner, which is a CGC due to the frame-shifted ORF. EWSR1-WT1 seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF. EWSR1-WT1 seems lost the major protein functional domain in Tgene partner, which is a CGC due to the frame-shifted ORF. EWSR1-WT1 seems lost the major protein functional domain in Tgene partner, which is a transcription factor due to the frame-shifted ORF. EWSR1-WT1 seems lost the major protein functional domain in Tgene partner, which is a tumor suppressor due to the frame-shifted ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Tgene	WT1	GO:0000122	negative regulation of transcription by RNA polymerase II	7585606
Tgene	WT1	GO:0007530	sex determination	9815658
Tgene	WT1	GO:0008285	negative regulation of cell proliferation	9553041\|9765217
Tgene	WT1	GO:0030308	negative regulation of cell growth	9553041\|9765217
Tgene	WT1	GO:0045892	negative regulation of transcription, DNA-templated	1332065\|7585606\|7720589\|8119964\|12802290\|14701728\|19050011
Tgene	WT1	GO:0045893	positive regulation of transcription, DNA-templated	8132626\|9178767\|9553041\|9765217\|12802290\|14701728\|16467207\|21390327
Tgene	WT1	GO:0045944	positive regulation of transcription by RNA polymerase II	23042785
Tgene	WT1	GO:0071371	cellular response to gonadotropin stimulus	15961562

Fusion gene breakpoints across EWSR1 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across WT1 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	desmoplastic small round cell tumor	S74529	EWSR1	chr22	29683128		WT1	chr11	32410699
ChimerKB3	.	.	EWSR1	chr22	29674205	+	WT1	chr11	32410725	-
ChimerKB3	.	.	EWSR1	chr22	29678546	+	WT1	chr11	32414301	-
ChimerKB3	.	.	EWSR1	chr22	29683128	+	WT1	chr11	32410699	-
ChimerKB3	.	.	EWSR1	chr22	29683128	+	WT1	chr11	32414261	-
ChimerKB4	.	.	EWSR1	chr22	29678546	+	WT1	chr11	32414301	-
ChiTaRS5.0	N/A	S74529	EWSR1	chr22	29683128	+	WT1	chr11	32414301	-
ChiTaRS5.0	N/A	S79672	EWSR1	chr22	29683128	+	WT1	chr11	32414301	-

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000332050	EWSR1	chr22	29674205	+	ENST00000332351	WT1	chr11	32410725	-	2146	741	362	862	166
ENST00000397938	EWSR1	chr22	29674205	+	ENST00000332351	WT1	chr11	32410725	-	2137	732	353	853	166
ENST00000406548	EWSR1	chr22	29674205	+	ENST00000332351	WT1	chr11	32410725	-	1882	477	98	598	166
ENST00000331029	EWSR1	chr22	29674205	+	ENST00000332351	WT1	chr11	32410725	-	1864	459	80	580	166
ENST00000414183	EWSR1	chr22	29674205	+	ENST00000332351	WT1	chr11	32410725	-	1857	452	467	0	156
ENST00000333395	EWSR1	chr22	29674205	+	ENST00000332351	WT1	chr11	32410725	-	1837	432	53	553	166
ENST00000332035	EWSR1	chr22	29674205	+	ENST00000332351	WT1	chr11	32410725	-	1829	424	45	545	166
ENST00000332050	EWSR1	chr22	29683128	+	ENST00000379079	WT1	chr11	32410699	-	2500	1121	328	1155	275
ENST00000332050	EWSR1	chr22	29683128	+	ENST00000530998	WT1	chr11	32410699	-	2497	1121	328	1155	275
ENST00000332050	EWSR1	chr22	29683128	+	ENST00000448076	WT1	chr11	32410699	-	1596	1121	328	1155	275
ENST00000397938	EWSR1	chr22	29683128	+	ENST00000379079	WT1	chr11	32410699	-	2491	1112	319	1146	275
ENST00000397938	EWSR1	chr22	29683128	+	ENST00000530998	WT1	chr11	32410699	-	2488	1112	319	1146	275
ENST00000397938	EWSR1	chr22	29683128	+	ENST00000448076	WT1	chr11	32410699	-	1587	1112	319	1146	275
ENST00000406548	EWSR1	chr22	29683128	+	ENST00000379079	WT1	chr11	32410699	-	2236	857	64	891	275
ENST00000406548	EWSR1	chr22	29683128	+	ENST00000530998	WT1	chr11	32410699	-	2233	857	64	891	275
ENST00000406548	EWSR1	chr22	29683128	+	ENST00000448076	WT1	chr11	32410699	-	1332	857	64	891	275
ENST00000331029	EWSR1	chr22	29683128	+	ENST00000379079	WT1	chr11	32410699	-	2218	839	46	873	275
ENST00000331029	EWSR1	chr22	29683128	+	ENST00000530998	WT1	chr11	32410699	-	2215	839	46	873	275
ENST00000331029	EWSR1	chr22	29683128	+	ENST00000448076	WT1	chr11	32410699	-	1314	839	46	873	275
ENST00000414183	EWSR1	chr22	29683128	+	ENST00000379079	WT1	chr11	32410699	-	2211	832	21	866	281
ENST00000414183	EWSR1	chr22	29683128	+	ENST00000530998	WT1	chr11	32410699	-	2208	832	21	866	281
ENST00000414183	EWSR1	chr22	29683128	+	ENST00000448076	WT1	chr11	32410699	-	1307	832	21	866	281
ENST00000333395	EWSR1	chr22	29683128	+	ENST00000379079	WT1	chr11	32410699	-	2191	812	19	846	275
ENST00000333395	EWSR1	chr22	29683128	+	ENST00000530998	WT1	chr11	32410699	-	2188	812	19	846	275
ENST00000333395	EWSR1	chr22	29683128	+	ENST00000448076	WT1	chr11	32410699	-	1287	812	19	846	275
ENST00000332035	EWSR1	chr22	29683128	+	ENST00000379079	WT1	chr11	32410699	-	2015	636	45	731	228
ENST00000332035	EWSR1	chr22	29683128	+	ENST00000530998	WT1	chr11	32410699	-	2012	636	45	731	228
ENST00000332035	EWSR1	chr22	29683128	+	ENST00000448076	WT1	chr11	32410699	-	1111	636	45	731	228
ENST00000332050	EWSR1	chr22	29683128	+	ENST00000379079	WT1	chr11	32414261	-	2660	1121	328	1242	304
ENST00000332050	EWSR1	chr22	29683128	+	ENST00000332351	WT1	chr11	32414261	-	2669	1121	328	1242	304
ENST00000332050	EWSR1	chr22	29683128	+	ENST00000530998	WT1	chr11	32414261	-	2666	1121	328	1242	304
ENST00000332050	EWSR1	chr22	29683128	+	ENST00000448076	WT1	chr11	32414261	-	1756	1121	328	1242	304
ENST00000397938	EWSR1	chr22	29683128	+	ENST00000379079	WT1	chr11	32414261	-	2651	1112	319	1233	304
ENST00000397938	EWSR1	chr22	29683128	+	ENST00000332351	WT1	chr11	32414261	-	2660	1112	319	1233	304
ENST00000397938	EWSR1	chr22	29683128	+	ENST00000530998	WT1	chr11	32414261	-	2657	1112	319	1233	304
ENST00000397938	EWSR1	chr22	29683128	+	ENST00000448076	WT1	chr11	32414261	-	1747	1112	319	1233	304
ENST00000406548	EWSR1	chr22	29683128	+	ENST00000379079	WT1	chr11	32414261	-	2396	857	64	978	304
ENST00000406548	EWSR1	chr22	29683128	+	ENST00000332351	WT1	chr11	32414261	-	2405	857	64	978	304
ENST00000406548	EWSR1	chr22	29683128	+	ENST00000530998	WT1	chr11	32414261	-	2402	857	64	978	304
ENST00000406548	EWSR1	chr22	29683128	+	ENST00000448076	WT1	chr11	32414261	-	1492	857	64	978	304
ENST00000331029	EWSR1	chr22	29683128	+	ENST00000379079	WT1	chr11	32414261	-	2378	839	46	960	304
ENST00000331029	EWSR1	chr22	29683128	+	ENST00000332351	WT1	chr11	32414261	-	2387	839	46	960	304
ENST00000331029	EWSR1	chr22	29683128	+	ENST00000530998	WT1	chr11	32414261	-	2384	839	46	960	304
ENST00000331029	EWSR1	chr22	29683128	+	ENST00000448076	WT1	chr11	32414261	-	1474	839	46	960	304
ENST00000414183	EWSR1	chr22	29683128	+	ENST00000379079	WT1	chr11	32414261	-	2371	832	21	953	310
ENST00000414183	EWSR1	chr22	29683128	+	ENST00000332351	WT1	chr11	32414261	-	2380	832	21	953	310
ENST00000414183	EWSR1	chr22	29683128	+	ENST00000530998	WT1	chr11	32414261	-	2377	832	21	953	310
ENST00000414183	EWSR1	chr22	29683128	+	ENST00000448076	WT1	chr11	32414261	-	1467	832	21	953	310
ENST00000333395	EWSR1	chr22	29683128	+	ENST00000379079	WT1	chr11	32414261	-	2351	812	19	933	304
ENST00000333395	EWSR1	chr22	29683128	+	ENST00000332351	WT1	chr11	32414261	-	2360	812	19	933	304
ENST00000333395	EWSR1	chr22	29683128	+	ENST00000530998	WT1	chr11	32414261	-	2357	812	19	933	304
ENST00000333395	EWSR1	chr22	29683128	+	ENST00000448076	WT1	chr11	32414261	-	1447	812	19	933	304
ENST00000332035	EWSR1	chr22	29683128	+	ENST00000379079	WT1	chr11	32414261	-	2175	636	11	757	248
ENST00000332035	EWSR1	chr22	29683128	+	ENST00000332351	WT1	chr11	32414261	-	2184	636	11	757	248
ENST00000332035	EWSR1	chr22	29683128	+	ENST00000530998	WT1	chr11	32414261	-	2181	636	11	757	248
ENST00000332035	EWSR1	chr22	29683128	+	ENST00000448076	WT1	chr11	32414261	-	1271	636	11	757	248
ENST00000332050	EWSR1	chr22	29683128		ENST00000379079	WT1	chr11	32410699		2500	1121	328	1155	275
ENST00000332050	EWSR1	chr22	29683128		ENST00000530998	WT1	chr11	32410699		2497	1121	328	1155	275
ENST00000332050	EWSR1	chr22	29683128		ENST00000448076	WT1	chr11	32410699		1596	1121	328	1155	275
ENST00000397938	EWSR1	chr22	29683128		ENST00000379079	WT1	chr11	32410699		2491	1112	319	1146	275
ENST00000397938	EWSR1	chr22	29683128		ENST00000530998	WT1	chr11	32410699		2488	1112	319	1146	275
ENST00000397938	EWSR1	chr22	29683128		ENST00000448076	WT1	chr11	32410699		1587	1112	319	1146	275
ENST00000406548	EWSR1	chr22	29683128		ENST00000379079	WT1	chr11	32410699		2236	857	64	891	275
ENST00000406548	EWSR1	chr22	29683128		ENST00000530998	WT1	chr11	32410699		2233	857	64	891	275
ENST00000406548	EWSR1	chr22	29683128		ENST00000448076	WT1	chr11	32410699		1332	857	64	891	275
ENST00000331029	EWSR1	chr22	29683128		ENST00000379079	WT1	chr11	32410699		2218	839	46	873	275
ENST00000331029	EWSR1	chr22	29683128		ENST00000530998	WT1	chr11	32410699		2215	839	46	873	275
ENST00000331029	EWSR1	chr22	29683128		ENST00000448076	WT1	chr11	32410699		1314	839	46	873	275
ENST00000414183	EWSR1	chr22	29683128		ENST00000379079	WT1	chr11	32410699		2211	832	21	866	281
ENST00000414183	EWSR1	chr22	29683128		ENST00000530998	WT1	chr11	32410699		2208	832	21	866	281
ENST00000414183	EWSR1	chr22	29683128		ENST00000448076	WT1	chr11	32410699		1307	832	21	866	281
ENST00000333395	EWSR1	chr22	29683128		ENST00000379079	WT1	chr11	32410699		2191	812	19	846	275
ENST00000333395	EWSR1	chr22	29683128		ENST00000530998	WT1	chr11	32410699		2188	812	19	846	275
ENST00000333395	EWSR1	chr22	29683128		ENST00000448076	WT1	chr11	32410699		1287	812	19	846	275
ENST00000332035	EWSR1	chr22	29683128		ENST00000379079	WT1	chr11	32410699		2015	636	45	731	228
ENST00000332035	EWSR1	chr22	29683128		ENST00000530998	WT1	chr11	32410699		2012	636	45	731	228
ENST00000332035	EWSR1	chr22	29683128		ENST00000448076	WT1	chr11	32410699		1111	636	45	731	228
ENST00000332050	EWSR1	chr22	29683128	+	ENST00000379079	WT1	chr11	32414301	-	2700	1121	328	1416	362
ENST00000332050	EWSR1	chr22	29683128	+	ENST00000332351	WT1	chr11	32414301	-	2709	1121	328	1425	365
ENST00000332050	EWSR1	chr22	29683128	+	ENST00000530998	WT1	chr11	32414301	-	2706	1121	328	1425	365
ENST00000332050	EWSR1	chr22	29683128	+	ENST00000448076	WT1	chr11	32414301	-	1796	1121	328	1416	362
ENST00000397938	EWSR1	chr22	29683128	+	ENST00000379079	WT1	chr11	32414301	-	2691	1112	319	1407	362
ENST00000397938	EWSR1	chr22	29683128	+	ENST00000332351	WT1	chr11	32414301	-	2700	1112	319	1416	365
ENST00000397938	EWSR1	chr22	29683128	+	ENST00000530998	WT1	chr11	32414301	-	2697	1112	319	1416	365
ENST00000397938	EWSR1	chr22	29683128	+	ENST00000448076	WT1	chr11	32414301	-	1787	1112	319	1407	362
ENST00000406548	EWSR1	chr22	29683128	+	ENST00000379079	WT1	chr11	32414301	-	2436	857	64	1152	362
ENST00000406548	EWSR1	chr22	29683128	+	ENST00000332351	WT1	chr11	32414301	-	2445	857	64	1161	365
ENST00000406548	EWSR1	chr22	29683128	+	ENST00000530998	WT1	chr11	32414301	-	2442	857	64	1161	365
ENST00000406548	EWSR1	chr22	29683128	+	ENST00000448076	WT1	chr11	32414301	-	1532	857	64	1152	362
ENST00000331029	EWSR1	chr22	29683128	+	ENST00000379079	WT1	chr11	32414301	-	2418	839	46	1134	362
ENST00000331029	EWSR1	chr22	29683128	+	ENST00000332351	WT1	chr11	32414301	-	2427	839	46	1143	365
ENST00000331029	EWSR1	chr22	29683128	+	ENST00000530998	WT1	chr11	32414301	-	2424	839	46	1143	365
ENST00000331029	EWSR1	chr22	29683128	+	ENST00000448076	WT1	chr11	32414301	-	1514	839	46	1134	362
ENST00000414183	EWSR1	chr22	29683128	+	ENST00000379079	WT1	chr11	32414301	-	2411	832	21	1127	368
ENST00000414183	EWSR1	chr22	29683128	+	ENST00000332351	WT1	chr11	32414301	-	2420	832	21	1136	371
ENST00000414183	EWSR1	chr22	29683128	+	ENST00000530998	WT1	chr11	32414301	-	2417	832	21	1136	371
ENST00000414183	EWSR1	chr22	29683128	+	ENST00000448076	WT1	chr11	32414301	-	1507	832	21	1127	368
ENST00000333395	EWSR1	chr22	29683128	+	ENST00000379079	WT1	chr11	32414301	-	2391	812	19	1107	362
ENST00000333395	EWSR1	chr22	29683128	+	ENST00000332351	WT1	chr11	32414301	-	2400	812	19	1116	365
ENST00000333395	EWSR1	chr22	29683128	+	ENST00000530998	WT1	chr11	32414301	-	2397	812	19	1116	365
ENST00000333395	EWSR1	chr22	29683128	+	ENST00000448076	WT1	chr11	32414301	-	1487	812	19	1107	362
ENST00000332035	EWSR1	chr22	29683128	+	ENST00000379079	WT1	chr11	32414301	-	2215	636	11	931	306
ENST00000332035	EWSR1	chr22	29683128	+	ENST00000332351	WT1	chr11	32414301	-	2224	636	11	940	309
ENST00000332035	EWSR1	chr22	29683128	+	ENST00000530998	WT1	chr11	32414301	-	2221	636	11	940	309
ENST00000332035	EWSR1	chr22	29683128	+	ENST00000448076	WT1	chr11	32414301	-	1311	636	11	931	306

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000332050	ENST00000379079	EWSR1	chr22	29683128		WT1	chr11	32410699		0.000484541	0.9995154
ENST00000332050	ENST00000530998	EWSR1	chr22	29683128		WT1	chr11	32410699		0.000491722	0.99950826
ENST00000332050	ENST00000448076	EWSR1	chr22	29683128		WT1	chr11	32410699		0.003160655	0.9968393
ENST00000397938	ENST00000379079	EWSR1	chr22	29683128		WT1	chr11	32410699		0.000440566	0.99955946
ENST00000397938	ENST00000530998	EWSR1	chr22	29683128		WT1	chr11	32410699		0.000442907	0.9995571
ENST00000397938	ENST00000448076	EWSR1	chr22	29683128		WT1	chr11	32410699		0.002748114	0.9972519
ENST00000406548	ENST00000379079	EWSR1	chr22	29683128		WT1	chr11	32410699		0.000394047	0.99960595
ENST00000406548	ENST00000530998	EWSR1	chr22	29683128		WT1	chr11	32410699		0.000396438	0.99960357
ENST00000406548	ENST00000448076	EWSR1	chr22	29683128		WT1	chr11	32410699		0.002610416	0.9973896
ENST00000331029	ENST00000379079	EWSR1	chr22	29683128		WT1	chr11	32410699		0.000377794	0.9996222
ENST00000331029	ENST00000530998	EWSR1	chr22	29683128		WT1	chr11	32410699		0.000380083	0.99961984
ENST00000331029	ENST00000448076	EWSR1	chr22	29683128		WT1	chr11	32410699		0.002517654	0.99748236
ENST00000414183	ENST00000379079	EWSR1	chr22	29683128		WT1	chr11	32410699		0.000382524	0.99961746
ENST00000414183	ENST00000530998	EWSR1	chr22	29683128		WT1	chr11	32410699		0.000383497	0.9996165
ENST00000414183	ENST00000448076	EWSR1	chr22	29683128		WT1	chr11	32410699		0.002347032	0.99765295
ENST00000333395	ENST00000379079	EWSR1	chr22	29683128		WT1	chr11	32410699		0.000320371	0.9996797
ENST00000333395	ENST00000530998	EWSR1	chr22	29683128		WT1	chr11	32410699		0.000322664	0.9996773
ENST00000333395	ENST00000448076	EWSR1	chr22	29683128		WT1	chr11	32410699		0.002115295	0.9978847
ENST00000332035	ENST00000379079	EWSR1	chr22	29683128		WT1	chr11	32410699		0.00042323	0.99957675
ENST00000332035	ENST00000530998	EWSR1	chr22	29683128		WT1	chr11	32410699		0.000428688	0.99957126
ENST00000332035	ENST00000448076	EWSR1	chr22	29683128		WT1	chr11	32410699		0.001255196	0.9987447
ENST00000332050	ENST00000379079	EWSR1	chr22	29683128	+	WT1	chr11	32414301	-	0.000374461	0.99962556
ENST00000332050	ENST00000332351	EWSR1	chr22	29683128	+	WT1	chr11	32414301	-	0.00039582	0.99960417
ENST00000332050	ENST00000530998	EWSR1	chr22	29683128	+	WT1	chr11	32414301	-	0.000401422	0.99959856
ENST00000332050	ENST00000448076	EWSR1	chr22	29683128	+	WT1	chr11	32414301	-	0.0047056	0.9952944
ENST00000397938	ENST00000379079	EWSR1	chr22	29683128	+	WT1	chr11	32414301	-	0.000354107	0.9996458
ENST00000397938	ENST00000332351	EWSR1	chr22	29683128	+	WT1	chr11	32414301	-	0.000378908	0.99962103
ENST00000397938	ENST00000530998	EWSR1	chr22	29683128	+	WT1	chr11	32414301	-	0.000383928	0.999616
ENST00000397938	ENST00000448076	EWSR1	chr22	29683128	+	WT1	chr11	32414301	-	0.00439791	0.99560213
ENST00000406548	ENST00000379079	EWSR1	chr22	29683128	+	WT1	chr11	32414301	-	0.000310685	0.99968934
ENST00000406548	ENST00000332351	EWSR1	chr22	29683128	+	WT1	chr11	32414301	-	0.000322711	0.9996773
ENST00000406548	ENST00000530998	EWSR1	chr22	29683128	+	WT1	chr11	32414301	-	0.00032796	0.99967206
ENST00000406548	ENST00000448076	EWSR1	chr22	29683128	+	WT1	chr11	32414301	-	0.005418076	0.9945819
ENST00000331029	ENST00000379079	EWSR1	chr22	29683128	+	WT1	chr11	32414301	-	0.000303208	0.99969685
ENST00000331029	ENST00000332351	EWSR1	chr22	29683128	+	WT1	chr11	32414301	-	0.000327626	0.9996724
ENST00000331029	ENST00000530998	EWSR1	chr22	29683128	+	WT1	chr11	32414301	-	0.000332575	0.9996674
ENST00000331029	ENST00000448076	EWSR1	chr22	29683128	+	WT1	chr11	32414301	-	0.005583379	0.9944166
ENST00000414183	ENST00000379079	EWSR1	chr22	29683128	+	WT1	chr11	32414301	-	0.00055507	0.9994449
ENST00000414183	ENST00000332351	EWSR1	chr22	29683128	+	WT1	chr11	32414301	-	0.000627354	0.99937266
ENST00000414183	ENST00000530998	EWSR1	chr22	29683128	+	WT1	chr11	32414301	-	0.000636564	0.9993635
ENST00000414183	ENST00000448076	EWSR1	chr22	29683128	+	WT1	chr11	32414301	-	0.009089387	0.9909106
ENST00000333395	ENST00000379079	EWSR1	chr22	29683128	+	WT1	chr11	32414301	-	0.000286996	0.999713
ENST00000333395	ENST00000332351	EWSR1	chr22	29683128	+	WT1	chr11	32414301	-	0.00030343	0.9996966
ENST00000333395	ENST00000530998	EWSR1	chr22	29683128	+	WT1	chr11	32414301	-	0.000308707	0.99969137
ENST00000333395	ENST00000448076	EWSR1	chr22	29683128	+	WT1	chr11	32414301	-	0.005186848	0.9948132
ENST00000332035	ENST00000379079	EWSR1	chr22	29683128	+	WT1	chr11	32414301	-	0.000681271	0.9993187
ENST00000332035	ENST00000332351	EWSR1	chr22	29683128	+	WT1	chr11	32414301	-	0.00091059	0.99908936
ENST00000332035	ENST00000530998	EWSR1	chr22	29683128	+	WT1	chr11	32414301	-	0.000926574	0.9990734
ENST00000332035	ENST00000448076	EWSR1	chr22	29683128	+	WT1	chr11	32414301	-	0.008574056	0.991426

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>27858_27858_1_EWSR1-WT1_EWSR1_chr22_29674205_ENST00000331029_WT1_chr11_32410725_ENST00000332351_length(amino acids)=166AA_BP=117
MQRSRATVLTPPSPLKDMHRPPRHMGNKAMEPMDSPLMSAIPRLRPLQPMGRPPMQLLMDSLPLVILLQLPPRHTASLSRGMALVLMIPP

--------------------------------------------------------------

>27858_27858_2_EWSR1-WT1_EWSR1_chr22_29674205_ENST00000332035_WT1_chr11_32410725_ENST00000332351_length(amino acids)=166AA_BP=117
MQRSRATVLTPPSPLKDMHRPPRHMGNKAMEPMDSPLMSAIPRLRPLQPMGRPPMQLLMDSLPLVILLQLPPRHTASLSRGMALVLMIPP

--------------------------------------------------------------

>27858_27858_3_EWSR1-WT1_EWSR1_chr22_29674205_ENST00000332050_WT1_chr11_32410725_ENST00000332351_length(amino acids)=166AA_BP=117
MQRSRATVLTPPSPLKDMHRPPRHMGNKAMEPMDSPLMSAIPRLRPLQPMGRPPMQLLMDSLPLVILLQLPPRHTASLSRGMALVLMIPP

--------------------------------------------------------------

>27858_27858_4_EWSR1-WT1_EWSR1_chr22_29674205_ENST00000333395_WT1_chr11_32410725_ENST00000332351_length(amino acids)=166AA_BP=117
MQRSRATVLTPPSPLKDMHRPPRHMGNKAMEPMDSPLMSAIPRLRPLQPMGRPPMQLLMDSLPLVILLQLPPRHTASLSRGMALVLMIPP

--------------------------------------------------------------

>27858_27858_5_EWSR1-WT1_EWSR1_chr22_29674205_ENST00000397938_WT1_chr11_32410725_ENST00000332351_length(amino acids)=166AA_BP=117
MQRSRATVLTPPSPLKDMHRPPRHMGNKAMEPMDSPLMSAIPRLRPLQPMGRPPMQLLMDSLPLVILLQLPPRHTASLSRGMALVLMIPP

--------------------------------------------------------------

>27858_27858_6_EWSR1-WT1_EWSR1_chr22_29674205_ENST00000406548_WT1_chr11_32410725_ENST00000332351_length(amino acids)=166AA_BP=117
MQRSRATVLTPPSPLKDMHRPPRHMGNKAMEPMDSPLMSAIPRLRPLQPMGRPPMQLLMDSLPLVILLQLPPRHTASLSRGMALVLMIPP

--------------------------------------------------------------

>27858_27858_7_EWSR1-WT1_EWSR1_chr22_29674205_ENST00000414183_WT1_chr11_32410725_ENST00000332351_length(amino acids)=156AA_BP=
MKGFSPCRCSGCWLLPIGWISRLSAICRLSCIGGLGGGDCSSGGIISTSAIPLDRLAVCLGGSWSSITCTGPFYSGRLSIRSCIGGLPIG

--------------------------------------------------------------

>27858_27858_8_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000331029_WT1_chr11_32410699_ENST00000379079_length(amino acids)=275AA_BP=
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQTKLSEK

--------------------------------------------------------------

>27858_27858_9_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000331029_WT1_chr11_32410699_ENST00000448076_length(amino acids)=275AA_BP=
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQTKLSEK

--------------------------------------------------------------

>27858_27858_10_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000331029_WT1_chr11_32410699_ENST00000530998_length(amino acids)=275AA_BP=
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQTKLSEK

--------------------------------------------------------------

>27858_27858_11_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000331029_WT1_chr11_32414261_ENST00000332351_length(amino acids)=304AA_BP=
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSEKPFS

--------------------------------------------------------------

>27858_27858_12_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000331029_WT1_chr11_32414261_ENST00000379079_length(amino acids)=304AA_BP=
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSEKPFS

--------------------------------------------------------------

>27858_27858_13_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000331029_WT1_chr11_32414261_ENST00000448076_length(amino acids)=304AA_BP=
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSEKPFS

--------------------------------------------------------------

>27858_27858_14_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000331029_WT1_chr11_32414261_ENST00000530998_length(amino acids)=304AA_BP=
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSEKPFS

--------------------------------------------------------------

>27858_27858_15_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000331029_WT1_chr11_32414301_ENST00000332351_length(amino acids)=365AA_BP=263
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSEKPYQ
CDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGKTSEKPFSCRWPSCQKKFARSDELVRHHNMHQRNMTK

--------------------------------------------------------------

>27858_27858_16_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000331029_WT1_chr11_32414301_ENST00000379079_length(amino acids)=362AA_BP=263
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSEKPYQ
CDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGEKPFSCRWPSCQKKFARSDELVRHHNMHQRNMTKLQL

--------------------------------------------------------------

>27858_27858_17_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000331029_WT1_chr11_32414301_ENST00000448076_length(amino acids)=362AA_BP=263
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSEKPYQ
CDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGEKPFSCRWPSCQKKFARSDELVRHHNMHQRNMTKLQL

--------------------------------------------------------------

>27858_27858_18_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000331029_WT1_chr11_32414301_ENST00000530998_length(amino acids)=365AA_BP=263
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSEKPYQ
CDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGKTSEKPFSCRWPSCQKKFARSDELVRHHNMHQRNMTK

--------------------------------------------------------------

>27858_27858_19_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000332035_WT1_chr11_32410699_ENST00000379079_length(amino acids)=228AA_BP=197
MQRSRATVLTPPSPLKDMHRPPRHMGNKAMEPMDSPLMSAIPRLRPLQPMGRPPMQLLMDSLPLVILLQLPPRHTASLSRGMALVLMIPP
LLQSPPPRPPMQLSLHMALSLLIQPMGSSQQPLHLQAIPLHSRLVMIRAVTLSRTPMGNRAAMDSRVAMVNKAAMGSSLPLVTHPKLDPT

--------------------------------------------------------------

>27858_27858_20_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000332035_WT1_chr11_32410699_ENST00000448076_length(amino acids)=228AA_BP=197
MQRSRATVLTPPSPLKDMHRPPRHMGNKAMEPMDSPLMSAIPRLRPLQPMGRPPMQLLMDSLPLVILLQLPPRHTASLSRGMALVLMIPP
LLQSPPPRPPMQLSLHMALSLLIQPMGSSQQPLHLQAIPLHSRLVMIRAVTLSRTPMGNRAAMDSRVAMVNKAAMGSSLPLVTHPKLDPT

--------------------------------------------------------------

>27858_27858_21_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000332035_WT1_chr11_32410699_ENST00000530998_length(amino acids)=228AA_BP=197
MQRSRATVLTPPSPLKDMHRPPRHMGNKAMEPMDSPLMSAIPRLRPLQPMGRPPMQLLMDSLPLVILLQLPPRHTASLSRGMALVLMIPP
LLQSPPPRPPMQLSLHMALSLLIQPMGSSQQPLHLQAIPLHSRLVMIRAVTLSRTPMGNRAAMDSRVAMVNKAAMGSSLPLVTHPKLDPT

--------------------------------------------------------------

>27858_27858_22_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000332035_WT1_chr11_32414261_ENST00000332351_length(amino acids)=248AA_BP=
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQP

--------------------------------------------------------------

>27858_27858_23_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000332035_WT1_chr11_32414261_ENST00000379079_length(amino acids)=248AA_BP=
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQP

--------------------------------------------------------------

>27858_27858_24_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000332035_WT1_chr11_32414261_ENST00000448076_length(amino acids)=248AA_BP=
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQP

--------------------------------------------------------------

>27858_27858_25_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000332035_WT1_chr11_32414261_ENST00000530998_length(amino acids)=248AA_BP=
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQP

--------------------------------------------------------------

>27858_27858_26_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000332035_WT1_chr11_32414301_ENST00000332351_length(amino acids)=309AA_BP=207
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQP
PTSYPPQTGSYSQAPSQYSQQSSSYGQQSEKPYQCDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGKTS

--------------------------------------------------------------

>27858_27858_27_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000332035_WT1_chr11_32414301_ENST00000379079_length(amino acids)=306AA_BP=207
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQP
PTSYPPQTGSYSQAPSQYSQQSSSYGQQSEKPYQCDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGEKP

--------------------------------------------------------------

>27858_27858_28_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000332035_WT1_chr11_32414301_ENST00000448076_length(amino acids)=306AA_BP=207
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQP
PTSYPPQTGSYSQAPSQYSQQSSSYGQQSEKPYQCDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGEKP

--------------------------------------------------------------

>27858_27858_29_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000332035_WT1_chr11_32414301_ENST00000530998_length(amino acids)=309AA_BP=207
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQP
PTSYPPQTGSYSQAPSQYSQQSSSYGQQSEKPYQCDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGKTS

--------------------------------------------------------------

>27858_27858_30_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000332050_WT1_chr11_32410699_ENST00000379079_length(amino acids)=275AA_BP=
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQTKLSEK

--------------------------------------------------------------

>27858_27858_31_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000332050_WT1_chr11_32410699_ENST00000448076_length(amino acids)=275AA_BP=
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQTKLSEK

--------------------------------------------------------------

>27858_27858_32_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000332050_WT1_chr11_32410699_ENST00000530998_length(amino acids)=275AA_BP=
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQTKLSEK

--------------------------------------------------------------

>27858_27858_33_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000332050_WT1_chr11_32414261_ENST00000332351_length(amino acids)=304AA_BP=
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSEKPFS

--------------------------------------------------------------

>27858_27858_34_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000332050_WT1_chr11_32414261_ENST00000379079_length(amino acids)=304AA_BP=
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSEKPFS

--------------------------------------------------------------

>27858_27858_35_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000332050_WT1_chr11_32414261_ENST00000448076_length(amino acids)=304AA_BP=
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSEKPFS

--------------------------------------------------------------

>27858_27858_36_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000332050_WT1_chr11_32414261_ENST00000530998_length(amino acids)=304AA_BP=
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSEKPFS

--------------------------------------------------------------

>27858_27858_37_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000332050_WT1_chr11_32414301_ENST00000332351_length(amino acids)=365AA_BP=263
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSEKPYQ
CDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGKTSEKPFSCRWPSCQKKFARSDELVRHHNMHQRNMTK

--------------------------------------------------------------

>27858_27858_38_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000332050_WT1_chr11_32414301_ENST00000379079_length(amino acids)=362AA_BP=263
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSEKPYQ
CDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGEKPFSCRWPSCQKKFARSDELVRHHNMHQRNMTKLQL

--------------------------------------------------------------

>27858_27858_39_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000332050_WT1_chr11_32414301_ENST00000448076_length(amino acids)=362AA_BP=263
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSEKPYQ
CDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGEKPFSCRWPSCQKKFARSDELVRHHNMHQRNMTKLQL

--------------------------------------------------------------

>27858_27858_40_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000332050_WT1_chr11_32414301_ENST00000530998_length(amino acids)=365AA_BP=263
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSEKPYQ
CDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGKTSEKPFSCRWPSCQKKFARSDELVRHHNMHQRNMTK

--------------------------------------------------------------

>27858_27858_41_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000333395_WT1_chr11_32410699_ENST00000379079_length(amino acids)=275AA_BP=
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQTKLSEK

--------------------------------------------------------------

>27858_27858_42_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000333395_WT1_chr11_32410699_ENST00000448076_length(amino acids)=275AA_BP=
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQTKLSEK

--------------------------------------------------------------

>27858_27858_43_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000333395_WT1_chr11_32410699_ENST00000530998_length(amino acids)=275AA_BP=
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQTKLSEK

--------------------------------------------------------------

>27858_27858_44_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000333395_WT1_chr11_32414261_ENST00000332351_length(amino acids)=304AA_BP=
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSEKPFS

--------------------------------------------------------------

>27858_27858_45_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000333395_WT1_chr11_32414261_ENST00000379079_length(amino acids)=304AA_BP=
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSEKPFS

--------------------------------------------------------------

>27858_27858_46_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000333395_WT1_chr11_32414261_ENST00000448076_length(amino acids)=304AA_BP=
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSEKPFS

--------------------------------------------------------------

>27858_27858_47_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000333395_WT1_chr11_32414261_ENST00000530998_length(amino acids)=304AA_BP=
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSEKPFS

--------------------------------------------------------------

>27858_27858_48_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000333395_WT1_chr11_32414301_ENST00000332351_length(amino acids)=365AA_BP=263
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSEKPYQ
CDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGKTSEKPFSCRWPSCQKKFARSDELVRHHNMHQRNMTK

--------------------------------------------------------------

>27858_27858_49_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000333395_WT1_chr11_32414301_ENST00000379079_length(amino acids)=362AA_BP=263
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSEKPYQ
CDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGEKPFSCRWPSCQKKFARSDELVRHHNMHQRNMTKLQL

--------------------------------------------------------------

>27858_27858_50_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000333395_WT1_chr11_32414301_ENST00000448076_length(amino acids)=362AA_BP=263
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSEKPYQ
CDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGEKPFSCRWPSCQKKFARSDELVRHHNMHQRNMTKLQL

--------------------------------------------------------------

>27858_27858_51_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000333395_WT1_chr11_32414301_ENST00000530998_length(amino acids)=365AA_BP=263
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSEKPYQ
CDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGKTSEKPFSCRWPSCQKKFARSDELVRHHNMHQRNMTK

--------------------------------------------------------------

>27858_27858_52_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000397938_WT1_chr11_32410699_ENST00000379079_length(amino acids)=275AA_BP=
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQTKLSEK

--------------------------------------------------------------

>27858_27858_53_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000397938_WT1_chr11_32410699_ENST00000448076_length(amino acids)=275AA_BP=
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQTKLSEK

--------------------------------------------------------------

>27858_27858_54_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000397938_WT1_chr11_32410699_ENST00000530998_length(amino acids)=275AA_BP=
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQTKLSEK

--------------------------------------------------------------

>27858_27858_55_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000397938_WT1_chr11_32414261_ENST00000332351_length(amino acids)=304AA_BP=
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSEKPFS

--------------------------------------------------------------

>27858_27858_56_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000397938_WT1_chr11_32414261_ENST00000379079_length(amino acids)=304AA_BP=
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSEKPFS

--------------------------------------------------------------

>27858_27858_57_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000397938_WT1_chr11_32414261_ENST00000448076_length(amino acids)=304AA_BP=
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSEKPFS

--------------------------------------------------------------

>27858_27858_58_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000397938_WT1_chr11_32414261_ENST00000530998_length(amino acids)=304AA_BP=
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSEKPFS

--------------------------------------------------------------

>27858_27858_59_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000397938_WT1_chr11_32414301_ENST00000332351_length(amino acids)=365AA_BP=263
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSEKPYQ
CDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGKTSEKPFSCRWPSCQKKFARSDELVRHHNMHQRNMTK

--------------------------------------------------------------

>27858_27858_60_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000397938_WT1_chr11_32414301_ENST00000379079_length(amino acids)=362AA_BP=263
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSEKPYQ
CDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGEKPFSCRWPSCQKKFARSDELVRHHNMHQRNMTKLQL

--------------------------------------------------------------

>27858_27858_61_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000397938_WT1_chr11_32414301_ENST00000448076_length(amino acids)=362AA_BP=263
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSEKPYQ
CDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGEKPFSCRWPSCQKKFARSDELVRHHNMHQRNMTKLQL

--------------------------------------------------------------

>27858_27858_62_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000397938_WT1_chr11_32414301_ENST00000530998_length(amino acids)=365AA_BP=263
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSEKPYQ
CDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGKTSEKPFSCRWPSCQKKFARSDELVRHHNMHQRNMTK

--------------------------------------------------------------

>27858_27858_63_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000406548_WT1_chr11_32410699_ENST00000379079_length(amino acids)=275AA_BP=
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQTKLSEK

--------------------------------------------------------------

>27858_27858_64_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000406548_WT1_chr11_32410699_ENST00000448076_length(amino acids)=275AA_BP=
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQTKLSEK

--------------------------------------------------------------

>27858_27858_65_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000406548_WT1_chr11_32410699_ENST00000530998_length(amino acids)=275AA_BP=
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQTKLSEK

--------------------------------------------------------------

>27858_27858_66_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000406548_WT1_chr11_32414261_ENST00000332351_length(amino acids)=304AA_BP=
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSEKPFS

--------------------------------------------------------------

>27858_27858_67_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000406548_WT1_chr11_32414261_ENST00000379079_length(amino acids)=304AA_BP=
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSEKPFS

--------------------------------------------------------------

>27858_27858_68_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000406548_WT1_chr11_32414261_ENST00000448076_length(amino acids)=304AA_BP=
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSEKPFS

--------------------------------------------------------------

>27858_27858_69_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000406548_WT1_chr11_32414261_ENST00000530998_length(amino acids)=304AA_BP=
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSEKPFS

--------------------------------------------------------------

>27858_27858_70_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000406548_WT1_chr11_32414301_ENST00000332351_length(amino acids)=365AA_BP=263
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSEKPYQ
CDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGKTSEKPFSCRWPSCQKKFARSDELVRHHNMHQRNMTK

--------------------------------------------------------------

>27858_27858_71_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000406548_WT1_chr11_32414301_ENST00000379079_length(amino acids)=362AA_BP=263
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSEKPYQ
CDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGEKPFSCRWPSCQKKFARSDELVRHHNMHQRNMTKLQL

--------------------------------------------------------------

>27858_27858_72_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000406548_WT1_chr11_32414301_ENST00000448076_length(amino acids)=362AA_BP=263
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSEKPYQ
CDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGEKPFSCRWPSCQKKFARSDELVRHHNMHQRNMTKLQL

--------------------------------------------------------------

>27858_27858_73_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000406548_WT1_chr11_32414301_ENST00000530998_length(amino acids)=365AA_BP=263
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPV
QGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYP
MQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSEKPYQ
CDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGKTSEKPFSCRWPSCQKKFARSDELVRHHNMHQRNMTK

--------------------------------------------------------------

>27858_27858_74_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000414183_WT1_chr11_32410699_ENST00000379079_length(amino acids)=281AA_BP=
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTVEGTSTGYTTPTAPQ
AYSQPVQGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQ
VPGSYPMQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQ

--------------------------------------------------------------

>27858_27858_75_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000414183_WT1_chr11_32410699_ENST00000448076_length(amino acids)=281AA_BP=
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTVEGTSTGYTTPTAPQ
AYSQPVQGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQ
VPGSYPMQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQ

--------------------------------------------------------------

>27858_27858_76_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000414183_WT1_chr11_32410699_ENST00000530998_length(amino acids)=281AA_BP=
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTVEGTSTGYTTPTAPQ
AYSQPVQGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQ
VPGSYPMQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQ

--------------------------------------------------------------

>27858_27858_77_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000414183_WT1_chr11_32414261_ENST00000332351_length(amino acids)=310AA_BP=
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTVEGTSTGYTTPTAPQ
AYSQPVQGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQ
VPGSYPMQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQ

--------------------------------------------------------------

>27858_27858_78_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000414183_WT1_chr11_32414261_ENST00000379079_length(amino acids)=310AA_BP=
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTVEGTSTGYTTPTAPQ
AYSQPVQGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQ
VPGSYPMQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQ

--------------------------------------------------------------

>27858_27858_79_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000414183_WT1_chr11_32414261_ENST00000448076_length(amino acids)=310AA_BP=
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTVEGTSTGYTTPTAPQ
AYSQPVQGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQ
VPGSYPMQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQ

--------------------------------------------------------------

>27858_27858_80_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000414183_WT1_chr11_32414261_ENST00000530998_length(amino acids)=310AA_BP=
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTVEGTSTGYTTPTAPQ
AYSQPVQGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQ
VPGSYPMQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQ

--------------------------------------------------------------

>27858_27858_81_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000414183_WT1_chr11_32414301_ENST00000332351_length(amino acids)=371AA_BP=269
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTVEGTSTGYTTPTAPQ
AYSQPVQGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQ
VPGSYPMQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQ
SEKPYQCDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGKTSEKPFSCRWPSCQKKFARSDELVRHHNMH

--------------------------------------------------------------

>27858_27858_82_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000414183_WT1_chr11_32414301_ENST00000379079_length(amino acids)=368AA_BP=269
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTVEGTSTGYTTPTAPQ
AYSQPVQGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQ
VPGSYPMQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQ
SEKPYQCDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGEKPFSCRWPSCQKKFARSDELVRHHNMHQRN

--------------------------------------------------------------

>27858_27858_83_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000414183_WT1_chr11_32414301_ENST00000448076_length(amino acids)=368AA_BP=269
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTVEGTSTGYTTPTAPQ
AYSQPVQGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQ
VPGSYPMQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQ
SEKPYQCDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGEKPFSCRWPSCQKKFARSDELVRHHNMHQRN

--------------------------------------------------------------

>27858_27858_84_EWSR1-WT1_EWSR1_chr22_29683128_ENST00000414183_WT1_chr11_32414301_ENST00000530998_length(amino acids)=371AA_BP=269
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTVEGTSTGYTTPTAPQ
AYSQPVQGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQ
VPGSYPMQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQ
SEKPYQCDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGKTSEKPFSCRWPSCQKKFARSDELVRHHNMH

--------------------------------------------------------------

Top

Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr22:29683128/chr11:32414301)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
EWSR1 Q01844	WT1 P19544
FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.	FUNCTION: Transcription factor that plays an important role in cellular development and cell survival (PubMed:7862533). Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3' (PubMed:7862533, PubMed:17716689, PubMed:25258363). Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors (PubMed:15520190). Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing (PubMed:16934801). Isoform 1 has lower affinity for DNA, and can bind RNA (PubMed:19123921). {ECO:0000269\|PubMed:15520190, ECO:0000269\|PubMed:16934801, ECO:0000269\|PubMed:17716689, ECO:0000269\|PubMed:19123921, ECO:0000269\|PubMed:19416806, ECO:0000269\|PubMed:25258363, ECO:0000269\|PubMed:7862533}.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000332035

111_116

208.33333333333334

601.0

Repeat

Note=12

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000332035

117_125

208.33333333333334

601.0

Repeat

Note=13

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000332035

126_156

208.33333333333334

601.0

Repeat

Note=14

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000332035

157_163

208.33333333333334

601.0

Repeat

Note=15

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000332035

164_170

208.33333333333334

601.0

Repeat

Note=16

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000332035

171_177

208.33333333333334

601.0

Repeat

Note=17

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000332035

178_188

208.33333333333334

601.0

Repeat

Note=18

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000332035

17_27

208.33333333333334

601.0

Repeat

Note=2

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000332035

189_193

208.33333333333334

601.0

Repeat

Note=19

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000332035

194_201

208.33333333333334

601.0

Repeat

Note=20

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000332035

202_206

208.33333333333334

601.0

Repeat

Note=21

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000332035

28_34

208.33333333333334

601.0

Repeat

Note=3

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000332035

35_42

208.33333333333334

601.0

Repeat

Note=4

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000332035

43_50

208.33333333333334

601.0

Repeat

Note=5

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000332035

51_59

208.33333333333334

601.0

Repeat

Note=6

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000332035

60_68

208.33333333333334

601.0

Repeat

Note=7

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000332035

69_75

208.33333333333334

601.0

Repeat

Note=8

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000332035

76_84

208.33333333333334

601.0

Repeat

Note=9

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000332035

85_91

208.33333333333334

601.0

Repeat

Note=10

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000332035

8_16

208.33333333333334

601.0

Repeat

Note=1

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000332035

92_110

208.33333333333334

601.0

Repeat

Note=11

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000333395

111_116

264.3333333333333

355.0

Repeat

Note=12

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000333395

117_125

264.3333333333333

355.0

Repeat

Note=13

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000333395

126_156

264.3333333333333

355.0

Repeat

Note=14

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000333395

157_163

264.3333333333333

355.0

Repeat

Note=15

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000333395

164_170

264.3333333333333

355.0

Repeat

Note=16

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000333395

171_177

264.3333333333333

355.0

Repeat

Note=17

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000333395

178_188

264.3333333333333

355.0

Repeat

Note=18

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000333395

17_27

264.3333333333333

355.0

Repeat

Note=2

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000333395

189_193

264.3333333333333

355.0

Repeat

Note=19

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000333395

194_201

264.3333333333333

355.0

Repeat

Note=20

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000333395

202_206

264.3333333333333

355.0

Repeat

Note=21

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000333395

207_212

264.3333333333333

355.0

Repeat

Note=22

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000333395

213_218

264.3333333333333

355.0

Repeat

Note=23

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000333395

219_224

264.3333333333333

355.0

Repeat

Note=24

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000333395

225_230

264.3333333333333

355.0

Repeat

Note=25

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000333395

231_238

264.3333333333333

355.0

Repeat

Note=26

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000333395

239_245

264.3333333333333

355.0

Repeat

Note=27

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000333395

246_252

264.3333333333333

355.0

Repeat

Note=28

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000333395

253_259

264.3333333333333

355.0

Repeat

Note=29

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000333395

28_34

264.3333333333333

355.0

Repeat

Note=3

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000333395

35_42

264.3333333333333

355.0

Repeat

Note=4

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000333395

43_50

264.3333333333333

355.0

Repeat

Note=5

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000333395

51_59

264.3333333333333

355.0

Repeat

Note=6

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000333395

60_68

264.3333333333333

355.0

Repeat

Note=7

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000333395

69_75

264.3333333333333

355.0

Repeat

Note=8

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000333395

76_84

264.3333333333333

355.0

Repeat

Note=9

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000333395

85_91

264.3333333333333

355.0

Repeat

Note=10

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000333395

8_16

264.3333333333333

355.0

Repeat

Note=1

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000333395

92_110

264.3333333333333

355.0

Repeat

Note=11

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000397938

111_116

264.3333333333333

657.0

Repeat

Note=12

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000397938

117_125

264.3333333333333

657.0

Repeat

Note=13

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000397938

126_156

264.3333333333333

657.0

Repeat

Note=14

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000397938

157_163

264.3333333333333

657.0

Repeat

Note=15

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000397938

164_170

264.3333333333333

657.0

Repeat

Note=16

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000397938

171_177

264.3333333333333

657.0

Repeat

Note=17

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000397938

178_188

264.3333333333333

657.0

Repeat

Note=18

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000397938

17_27

264.3333333333333

657.0

Repeat

Note=2

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000397938

189_193

264.3333333333333

657.0

Repeat

Note=19

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000397938

194_201

264.3333333333333

657.0

Repeat

Note=20

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000397938

202_206

264.3333333333333

657.0

Repeat

Note=21

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000397938

207_212

264.3333333333333

657.0

Repeat

Note=22

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000397938

213_218

264.3333333333333

657.0

Repeat

Note=23

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000397938

219_224

264.3333333333333

657.0

Repeat

Note=24

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000397938

225_230

264.3333333333333

657.0

Repeat

Note=25

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000397938

231_238

264.3333333333333

657.0

Repeat

Note=26

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000397938

239_245

264.3333333333333

657.0

Repeat

Note=27

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000397938

246_252

264.3333333333333

657.0

Repeat

Note=28

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000397938

253_259

264.3333333333333

657.0

Repeat

Note=29

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000397938

28_34

264.3333333333333

657.0

Repeat

Note=3

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000397938

35_42

264.3333333333333

657.0

Repeat

Note=4

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000397938

43_50

264.3333333333333

657.0

Repeat

Note=5

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000397938

51_59

264.3333333333333

657.0

Repeat

Note=6

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000397938

60_68

264.3333333333333

657.0

Repeat

Note=7

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000397938

69_75

264.3333333333333

657.0

Repeat

Note=8

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000397938

76_84

264.3333333333333

657.0

Repeat

Note=9

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000397938

85_91

264.3333333333333

657.0

Repeat

Note=10

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000397938

8_16

264.3333333333333

657.0

Repeat

Note=1

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000397938

92_110

264.3333333333333

657.0

Repeat

Note=11

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000406548

111_116

264.3333333333333

656.0

Repeat

Note=12

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000406548

117_125

264.3333333333333

656.0

Repeat

Note=13

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000406548

126_156

264.3333333333333

656.0

Repeat

Note=14

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000406548

157_163

264.3333333333333

656.0

Repeat

Note=15

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000406548

164_170

264.3333333333333

656.0

Repeat

Note=16

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000406548

171_177

264.3333333333333

656.0

Repeat

Note=17

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000406548

178_188

264.3333333333333

656.0

Repeat

Note=18

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000406548

17_27

264.3333333333333

656.0

Repeat

Note=2

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000406548

189_193

264.3333333333333

656.0

Repeat

Note=19

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000406548

194_201

264.3333333333333

656.0

Repeat

Note=20

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000406548

202_206

264.3333333333333

656.0

Repeat

Note=21

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000406548

207_212

264.3333333333333

656.0

Repeat

Note=22

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000406548

213_218

264.3333333333333

656.0

Repeat

Note=23

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000406548

219_224

264.3333333333333

656.0

Repeat

Note=24

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000406548

225_230

264.3333333333333

656.0

Repeat

Note=25

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000406548

231_238

264.3333333333333

656.0

Repeat

Note=26

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000406548

239_245

264.3333333333333

656.0

Repeat

Note=27

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000406548

246_252

264.3333333333333

656.0

Repeat

Note=28

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000406548

253_259

264.3333333333333

656.0

Repeat

Note=29

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000406548

28_34

264.3333333333333

656.0

Repeat

Note=3

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000406548

35_42

264.3333333333333

656.0

Repeat

Note=4

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000406548

43_50

264.3333333333333

656.0

Repeat

Note=5

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000406548

51_59

264.3333333333333

656.0

Repeat

Note=6

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000406548

60_68

264.3333333333333

656.0

Repeat

Note=7

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000406548

69_75

264.3333333333333

656.0

Repeat

Note=8

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000406548

76_84

264.3333333333333

656.0

Repeat

Note=9

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000406548

85_91

264.3333333333333

656.0

Repeat

Note=10

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000406548

8_16

264.3333333333333

656.0

Repeat

Note=1

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000406548

92_110

264.3333333333333

656.0

Repeat

Note=11

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000414183

111_116

270.3333333333333

662.0

Repeat

Note=12

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000414183

117_125

270.3333333333333

662.0

Repeat

Note=13

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000414183

126_156

270.3333333333333

662.0

Repeat

Note=14

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000414183

157_163

270.3333333333333

662.0

Repeat

Note=15

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000414183

164_170

270.3333333333333

662.0

Repeat

Note=16

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000414183

171_177

270.3333333333333

662.0

Repeat

Note=17

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000414183

178_188

270.3333333333333

662.0

Repeat

Note=18

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000414183

17_27

270.3333333333333

662.0

Repeat

Note=2

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000414183

189_193

270.3333333333333

662.0

Repeat

Note=19

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000414183

194_201

270.3333333333333

662.0

Repeat

Note=20

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000414183

202_206

270.3333333333333

662.0

Repeat

Note=21

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000414183

207_212

270.3333333333333

662.0

Repeat

Note=22

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000414183

213_218

270.3333333333333

662.0

Repeat

Note=23

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000414183

219_224

270.3333333333333

662.0

Repeat

Note=24

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000414183

225_230

270.3333333333333

662.0

Repeat

Note=25

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000414183

231_238

270.3333333333333

662.0

Repeat

Note=26

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000414183

239_245

270.3333333333333

662.0

Repeat

Note=27

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000414183

246_252

270.3333333333333

662.0

Repeat

Note=28

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000414183

253_259

270.3333333333333

662.0

Repeat

Note=29

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000414183

28_34

270.3333333333333

662.0

Repeat

Note=3

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000414183

35_42

270.3333333333333

662.0

Repeat

Note=4

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000414183

43_50

270.3333333333333

662.0

Repeat

Note=5

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000414183

51_59

270.3333333333333

662.0

Repeat

Note=6

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000414183

60_68

270.3333333333333

662.0

Repeat

Note=7

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000414183

69_75

270.3333333333333

662.0

Repeat

Note=8

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000414183

76_84

270.3333333333333

662.0

Repeat

Note=9

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000414183

85_91

270.3333333333333

662.0

Repeat

Note=10

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000414183

8_16

270.3333333333333

662.0

Repeat

Note=1

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000414183

92_110

270.3333333333333

662.0

Repeat

Note=11

Tgene

WT1

chr22:29683128

chr11:32414301

ENST00000379079

236_244

204.33333333333334

303.0

Motif

9aaTAD

Tgene

WT1

chr22:29683128

chr11:32414301

ENST00000530998

236_244

187.33333333333334

289.0

Motif

9aaTAD

Tgene

WT1

chr22:29683128

chr11:32414301

ENST00000379079

323_347

204.33333333333334

303.0

Zinc finger

C2H2-type 1

Tgene

WT1

chr22:29683128

chr11:32414301

ENST00000379079

353_377

204.33333333333334

303.0

Zinc finger

C2H2-type 2

Tgene

WT1

chr22:29683128

chr11:32414301

ENST00000379079

383_405

204.33333333333334

303.0

Zinc finger

C2H2-type 3

Tgene

WT1

chr22:29683128

chr11:32414301

ENST00000379079

414_438

204.33333333333334

303.0

Zinc finger

C2H2-type 4

Tgene

WT1

chr22:29683128

chr11:32414301

ENST00000530998

323_347

187.33333333333334

289.0

Zinc finger

C2H2-type 1

Tgene

WT1

chr22:29683128

chr11:32414301

ENST00000530998

353_377

187.33333333333334

289.0

Zinc finger

C2H2-type 2

Tgene

WT1

chr22:29683128

chr11:32414301

ENST00000530998

383_405

187.33333333333334

289.0

Zinc finger

C2H2-type 3

Tgene

WT1

chr22:29683128

chr11:32414301

ENST00000530998

414_438

187.33333333333334

289.0

Zinc finger

C2H2-type 4

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000332035

300_340

208.33333333333334

601.0

Compositional bias

Note=Arg/Gly/Pro-rich

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000332035

454_513

208.33333333333334

601.0

Compositional bias

Note=Arg/Gly/Pro-rich

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000332035

559_640

208.33333333333334

601.0

Compositional bias

Note=Arg/Gly/Pro-rich

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000333395

300_340

264.3333333333333

355.0

Compositional bias

Note=Arg/Gly/Pro-rich

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000333395

454_513

264.3333333333333

355.0

Compositional bias

Note=Arg/Gly/Pro-rich

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000333395

559_640

264.3333333333333

355.0

Compositional bias

Note=Arg/Gly/Pro-rich

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000397938

300_340

264.3333333333333

657.0

Compositional bias

Note=Arg/Gly/Pro-rich

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000397938

454_513

264.3333333333333

657.0

Compositional bias

Note=Arg/Gly/Pro-rich

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000397938

559_640

264.3333333333333

657.0

Compositional bias

Note=Arg/Gly/Pro-rich

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000406548

300_340

264.3333333333333

656.0

Compositional bias

Note=Arg/Gly/Pro-rich

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000406548

454_513

264.3333333333333

656.0

Compositional bias

Note=Arg/Gly/Pro-rich

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000406548

559_640

264.3333333333333

656.0

Compositional bias

Note=Arg/Gly/Pro-rich

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000414183

300_340

270.3333333333333

662.0

Compositional bias

Note=Arg/Gly/Pro-rich

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000414183

454_513

270.3333333333333

662.0

Compositional bias

Note=Arg/Gly/Pro-rich

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000414183

559_640

270.3333333333333

662.0

Compositional bias

Note=Arg/Gly/Pro-rich

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000332035

256_285

208.33333333333334

601.0

Domain

Note=IQ

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000332035

361_447

208.33333333333334

601.0

Domain

RRM

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000333395

256_285

264.3333333333333

355.0

Domain

Note=IQ

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000333395

361_447

264.3333333333333

355.0

Domain

RRM

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000397938

256_285

264.3333333333333

657.0

Domain

Note=IQ

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000397938

361_447

264.3333333333333

657.0

Domain

RRM

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000406548

256_285

264.3333333333333

656.0

Domain

Note=IQ

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000406548

361_447

264.3333333333333

656.0

Domain

RRM

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000414183

256_285

270.3333333333333

662.0

Domain

Note=IQ

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000414183

361_447

270.3333333333333

662.0

Domain

RRM

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000332035

639_656

208.33333333333334

601.0

Motif

Nuclear localization signal

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000333395

639_656

264.3333333333333

355.0

Motif

Nuclear localization signal

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000397938

639_656

264.3333333333333

657.0

Motif

Nuclear localization signal

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000406548

639_656

264.3333333333333

656.0

Motif

Nuclear localization signal

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000414183

639_656

270.3333333333333

662.0

Motif

Nuclear localization signal

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000332035

1_285

208.33333333333334

601.0

Region

Note=EAD (Gln/Pro/Thr-rich)

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000332035

8_285

208.33333333333334

601.0

Region

Note=31 X approximate tandem repeats

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000333395

1_285

264.3333333333333

355.0

Region

Note=EAD (Gln/Pro/Thr-rich)

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000333395

8_285

264.3333333333333

355.0

Region

Note=31 X approximate tandem repeats

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000397938

1_285

264.3333333333333

657.0

Region

Note=EAD (Gln/Pro/Thr-rich)

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000397938

8_285

264.3333333333333

657.0

Region

Note=31 X approximate tandem repeats

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000406548

1_285

264.3333333333333

656.0

Region

Note=EAD (Gln/Pro/Thr-rich)

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000406548

8_285

264.3333333333333

656.0

Region

Note=31 X approximate tandem repeats

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000414183

1_285

270.3333333333333

662.0

Region

Note=EAD (Gln/Pro/Thr-rich)

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000414183

8_285

270.3333333333333

662.0

Region

Note=31 X approximate tandem repeats

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000332035

207_212

208.33333333333334

601.0

Repeat

Note=22

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000332035

213_218

208.33333333333334

601.0

Repeat

Note=23

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000332035

219_224

208.33333333333334

601.0

Repeat

Note=24

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000332035

225_230

208.33333333333334

601.0

Repeat

Note=25

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000332035

231_238

208.33333333333334

601.0

Repeat

Note=26

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000332035

239_245

208.33333333333334

601.0

Repeat

Note=27

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000332035

246_252

208.33333333333334

601.0

Repeat

Note=28

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000332035

253_259

208.33333333333334

601.0

Repeat

Note=29

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000332035

260_276

208.33333333333334

601.0

Repeat

Note=30

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000332035

277_285

208.33333333333334

601.0

Repeat

Note=31

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000333395

260_276

264.3333333333333

355.0

Repeat

Note=30

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000333395

277_285

264.3333333333333

355.0

Repeat

Note=31

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000397938

260_276

264.3333333333333

657.0

Repeat

Note=30

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000397938

277_285

264.3333333333333

657.0

Repeat

Note=31

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000406548

260_276

264.3333333333333

656.0

Repeat

Note=30

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000406548

277_285

264.3333333333333

656.0

Repeat

Note=31

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000414183

260_276

270.3333333333333

662.0

Repeat

Note=30

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000414183

277_285

270.3333333333333

662.0

Repeat

Note=31

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000332035

518_549

208.33333333333334

601.0

Zinc finger

RanBP2-type

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000333395

518_549

264.3333333333333

355.0

Zinc finger

RanBP2-type

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000397938

518_549

264.3333333333333

657.0

Zinc finger

RanBP2-type

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000406548

518_549

264.3333333333333

656.0

Zinc finger

RanBP2-type

Hgene

EWSR1

chr22:29683128

chr11:32414301

ENST00000414183

518_549

270.3333333333333

662.0

Zinc finger

RanBP2-type

Tgene

WT1

chr22:29683128

chr11:32414301

ENST00000379079

27_83

204.33333333333334

303.0

Compositional bias

Note=Pro-rich

Tgene

WT1

chr22:29683128

chr11:32414301

ENST00000530998

27_83

187.33333333333334

289.0

Compositional bias

Note=Pro-rich

Top

Fusion Protein Structures

PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.

Fusion protein PDB link (fusion AA seq ID in FusionPDB)	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	AA seq	Len(AA seq)

PDB file (11) >>>11.pdbFusion protein BP residue: CIF file (11) >>>11.cif	EWSR1	chr22	29674205	+	WT1	chr11	32410725	-	MKGFSPCRCSGCWLLPIGWISRLSAICRLSCIGGLGGGDCSSGGIISTSA IPLDRLAVCLGGSWSSITCTGPFYSGRLSIRSCIGGLPIGCSGLSLGIAD ISGLSIGSIALLPICLGGLCISLSGLGGVSTVALLRCSLAIGTVIRGRHF	156
3D view using mol* of 11 (AA BP:)


PDB file (14) >>>14.pdbFusion protein BP residue: 117 CIF file (14) >>>14.cif	EWSR1	chr22	29674205	+	WT1	chr11	32410725	-	MQRSRATVLTPPSPLKDMHRPPRHMGNKAMEPMDSPLMSAIPRLRPLQPM GRPPMQLLMDSLPLVILLQLPPRHTASLSRGMALVLMIPPLLQSPPPRPP MQLSLHMALSLLIQPMGSSQQPLHLQGEKPFSCRWPSCQKKFARSDELVR	166
3D view using mol* of 14 (AA BP:117)


PDB file (35) >>>35.pdbFusion protein BP residue: 197 CIF file (35) >>>35.cif	EWSR1	chr22	29683128	+	WT1	chr11	32410699	-	MQRSRATVLTPPSPLKDMHRPPRHMGNKAMEPMDSPLMSAIPRLRPLQPM GRPPMQLLMDSLPLVILLQLPPRHTASLSRGMALVLMIPPLLQSPPPRPP MQLSLHMALSLLIQPMGSSQQPLHLQAIPLHSRLVMIRAVTLSRTPMGNR AAMDSRVAMVNKAAMGSSLPLVTHPKLDPTAKLQVNIANRAAATGSRPSC	228
3D view using mol* of 35 (AA BP:197)


PDB file (43) >>>43.pdbFusion protein BP residue: CIF file (43) >>>43.cif	EWSR1	chr22	29683128	+	WT1	chr11	32414261	-	MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDV SYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPVQGYGTGAYDT TTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTSYSSTQPTSYDQS SYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQ	248
3D view using mol* of 43 (AA BP:)


PDB file (49) >>>49.pdbFusion protein BP residue: CIF file (49) >>>49.cif	EWSR1	chr22	29683128	+	WT1	chr11	32410699	-	MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDV SYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPVQGYGTGAYDT TTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQ PQSSTGGYNQPSLGYGQSNYSYPQVPGSYPMQPVTAPPSYPPTSYSSTQP TSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQA	275
3D view using mol* of 49 (AA BP:)


PDB file (51) >>>51.pdbFusion protein BP residue: CIF file (51) >>>51.cif	EWSR1	chr22	29683128	+	WT1	chr11	32410699	-	MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDV SYTQAQTTATYGQTAYATSYGQPPTVEGTSTGYTTPTAPQAYSQPVQGYG TGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNK PTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYPMQPVTAPPSYPPTS YSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQT	281
3D view using mol* of 51 (AA BP:)


PDB file (65) >>>65.pdbFusion protein BP residue: CIF file (65) >>>65.cif	EWSR1	chr22	29683128	+	WT1	chr11	32414261	-	MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDV SYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPVQGYGTGAYDT TTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQ PQSSTGGYNQPSLGYGQSNYSYPQVPGSYPMQPVTAPPSYPPTSYSSTQP TSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQA PSQYSQQSSSYGQQSEKPFSCRWPSCQKKFARSDELVRHHNMHQRNMTKL	304
3D view using mol* of 65 (AA BP:)


PDB file (70) >>>70.pdbFusion protein BP residue: CIF file (70) >>>70.cif	EWSR1	chr22	29683128	+	WT1	chr11	32414261	-	MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDV SYTQAQTTATYGQTAYATSYGQPPTVEGTSTGYTTPTAPQAYSQPVQGYG TGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNK PTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYPMQPVTAPPSYPPTS YSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQT GSYSQAPSQYSQQSSSYGQQSEKPFSCRWPSCQKKFARSDELVRHHNMHQ	310
3D view using mol* of 70 (AA BP:)

Top

pLDDT score distribution

pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

EWSR1_pLDDT.png

WT1_pLDDT.png

pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

EWSR1_WT1_11_pLDDT.png (AA BP:)

EWSR1_WT1_11_pLDDT_and_active_sites.png (AA BP:)

EWSR1_WT1_11_violinplot.png (AA BP:)

EWSR1_WT1_14_pLDDT.png (AA BP:117)

EWSR1_WT1_14_pLDDT_and_active_sites.png (AA BP:117)

EWSR1_WT1_14_violinplot.png (AA BP:117)

EWSR1_WT1_35_PAE.png (AA BP:197)

EWSR1_WT1_35_pLDDT.png (AA BP:197)

EWSR1_WT1_35_pLDDT_and_active_sites.png (AA BP:197)

EWSR1_WT1_35_violinplot.png (AA BP:197)

EWSR1_WT1_43_PAE.png (AA BP:)

EWSR1_WT1_43_pLDDT.png (AA BP:)

EWSR1_WT1_43_pLDDT_and_active_sites.png (AA BP:)

EWSR1_WT1_43_violinplot.png (AA BP:)

EWSR1_WT1_49_PAE.png (AA BP:)

EWSR1_WT1_49_pLDDT.png (AA BP:)

EWSR1_WT1_51_PAE.png (AA BP:)

EWSR1_WT1_51_pLDDT.png (AA BP:)

EWSR1_WT1_65_PAE.png (AA BP:)

EWSR1_WT1_65_pLDDT.png (AA BP:)

EWSR1_WT1_65_pLDDT_and_active_sites.png (AA BP:)

EWSR1_WT1_65_violinplot.png (AA BP:)

EWSR1_WT1_70_PAE.png (AA BP:)

EWSR1_WT1_70_pLDDT.png (AA BP:)

EWSR1_WT1_70_pLDDT_and_active_sites.png (AA BP:)

EWSR1_WT1_70_violinplot.png (AA BP:)

Top

Ramachandran Plot of Fusion Protein Structure

Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.

Fusion AA seq ID in FusionPDB and their Ramachandran plots

EWSR1_WT1_11.png

EWSR1_WT1_14.png

EWSR1_WT1_35.png

EWSR1_WT1_43.png

EWSR1_WT1_49.png

EWSR1_WT1_51.png

EWSR1_WT1_65.png

EWSR1_WT1_70.png

Top

Potential Active Site Information

The potential binding sites of these fusion proteins were identified using SiteMap, a module of the Schrodinger suite.

Fusion AA seq ID in FusionPDB	Site score	Size	D score	Volume	Exposure	Enclosure	Contact	Phobic	Philic	Balance	Don/Acc	Residues
11	1.033	193	1.101	668.85	0.613	0.65	0.853	0.864	0.702	1.231	0.825	Chain A: 4,5,6,7,8,9,10,11,12,13,14,15,16,17,19,20 ,21,22,23,24,29,40,47,49,50,51,52,53,54,56,57,58,5 9,60,61,62,64,81
14	0.512	15	0.319	38.416	0.643	0.64	1.001	0.428	1.315	0.326	0.518	Chain A: 135,137,154,155,158,159,162
35	0.795	67	0.784	149.205	0.647	0.59	0.818	0.188	1.04	0.181	0.56	Chain A: 195,196,197,198,200,210,213,214,216,217,2 18,220,221,224
43	0.467	16	0.365	35.672	0.729	0.558	0.832	0.439	1.06	0.414	0.209	Chain A: 215,216,217,230,231,234
65	0.531	22	0.455	79.919	0.786	0.566	0.786	0.289	0.997	0.29	0.549	Chain A: 258,259,260,261,262,263,267,278,279,280,2 89
70	0.497	15	0.433	52.136	0.797	0.546	0.714	0.222	0.792	0.28	0.883	Chain A: 264,265,266,267,269,273

Top

Potentially Interacting Small Molecules through Virtual Screening

The FDA-approved small molecule library molecules were subjected to virtual screening using the Glide.

Fusion AA seq ID in FusionPDB

ZINC ID

DrugBank ID

Drug name

Docking score

Glide gscore

Top

Drug information from DrugBank of the top 20 interacting small molecules.

ZINC ID

DrugBank ID

Drug name

Drug type

SMILES

Drug group

Top

Biochemical Features of Small Molecules

ADME (Absorption, Distribution, Metabolism, and Excretion) of drugs using QikProp(v3.9)

ZINC ID

mol_MW

dipole

SASA

FOSA

FISA

PISA

WPSA

volume

donorHB

accptHB

Human Oral Absorption

Percent Human Oral Absorption

Rule Of Five

Rule Of Three

Top

Drug Toxicity Information

Toxicity information of individual drugs using eToxPred

ZINC ID

Smile

Surface Accessibility

Toxicity

Top

Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

EWSR1

PRTFDC1, ZDHHC3, MSC, SLC22A24, MYO1F, KXD1, KHDRBS2, DNAJB3, FASN, NLE1, MNS1, PRUNE2, WWP2, NDUFB1, BNIP3L, NSUN4, KRR1, WWP1, RMND5B, SLC1A1, RASL11B, DFFA, WDR37, RPS15A, CPSF6, C11orf16, YY1AP1, RNF183, MTCP1, TULP2, RBPMS, KEL, MYOZ2, FAM131C, HMGA1, NPPB, HERPUD1, CD177, RPL31, VPS72, ACTL6A, RAD23A, MAGEA11, CFDP1, DMRTB1, CXADR, ZNF165, SSBP2, TPGS2, RAB37, CETP, NDUFV1, DYNLL2, NBPF3, CEACAM5, GPBP1L1, SERP2, GNPDA1, C19orf57, ELAVL3, ELAVL4, LILRA3, BAD, CCDC7, MRPS18B, CUEDC2, CNST, TSPAN3, CCDC91, TRIM37, NINL, NTNG2, CPSF7, PGLS, EPT1, MYL6, SMAD4, TMSB4Y, TRPV5, MVK, MAPK1IP1L, MDFI, MTMR9, PLSCR1, RALYL, PDHX, C10orf12, RHOXF2, MATK, SALL2, AGT, KCNMB1, SUV39H2, SMNDC1, ARHGDIA, PUF60, GSK3B, ILK, CD2BP2, BARD1, CREBBP, BTK, SF1, SNRPC, ELK1, PTK2B, CALM1, POU4F1, EZH2, IRF3, TONSL, RFX3, HLTF, SUPT4H1, ZNF184, HIST1H2BN, BLZF1, HDAC3, FXR2, HMGN4, POLR3A, ECD, ZBTB1, SCMH1, SUZ12, E2F8, TRIM5, ZNF383, DHX9, SMN1, PCM1, RAD21, NDRG1, CEBPA, ELAVL1, SIRT7, HNRNPA1, TSG101, TP53, POLR2A, YBX1, TDRD3, CUL3, CUL4A, CUL4B, CUL5, CUL2, CUL1, COPS5, COPS6, DCUN1D1, CAND1, NEDD8, KCND3, ATN1, ATXN3, ERCC5, HBP1, HSPA2, PCBP1, USP7, SRSF5, TMEM126A, SAP30BP, GORASP2, MBD3, MRPS9, HAX1, SFXN1, ITGA5, TCIRG1, RNF168, GEMIN5, HAS1, MTCH1, NDUFA5, MCAT, MRPL57, HDAC2, ESR1, FN1, VCAM1, ITGA4, CD81, PRMT1, SF3B4, EP300, PRRC2A, EWSR1, FUS, ITGB5, NONO, TRAF3, HNRNPUL1, EPAS1, CHERP, CDK12, ITCH, WBP4, rev, RPA3, RPA2, RPA1, HSPA5, RIOK2, TRAF1, TRAF2, SEC24D, TFG, SEC24A, SSBP3, PRR13, ATPAF2, PEF1, JUN, CUL7, OBSL1, CCDC8, RNF2, BMI1, EGFR, ABL1, SRPK1, ABCE1, PRMT8, RPS6KB2, ACAA2, ACAT1, EIF4H, ANXA2, HIP1R, PICALM, POR, NTRK1, SCARNA22, NPM1, KRT2, PRDX2, S100A9, YWHAZ, DDX17, PCLO, ANXA6, SEC24C, GIPC1, CSRP2, FHL1, HNRNPA3, CTTN, MARCKSL1, PARP1, SERPINB3, CCDC50, KIF2A, KRT6B, HIST1H1D, STK38, H2AFV, PACSIN2, U2AF1, RSL24D1, XRCC5, ESYT3, RPL29, SDF2L1, LRP1B, MSN, SEC23A, RPL7A, SNX18, PPIL1, RPS27A, IGHM, SUMO3, GTF2I, RUVBL1, KRT16, RBM8A, RPL8, SRSF9, ZC3HAV1L, GAPDH, ETV3, IGF2R, COL5A2, HNRNPD, ANP32B, WAC, TFAP2A, TTN, CBR3, ARGLU1, HNRNPAB, SRRT, ATP1B3, COPS7B, PRR12, ATF3, NOMO3, NOMO1, NOMO2, EYA3, C1orf198, MAZ, U2AF2, SSB, TRA2B, C1orf52, HMGB1, HMGB1P10, RPRD1A, HNRNPH3, EIF5A2, C1orf131, SEC13, MAPRE1, CSTF2T, SRSF3, LENG1, UPF1, HDGFRP2, RPS10P7, RPS10P11, RPS10, RPS10P13, RPS10P4, RPS10P22, DACH1, ANO1, NCOR1, MLX, SUMO1P3, SUMO1, WIZ, PFDN6, ARFIP2, ZHX3, EEF1B2, MBIP, BAG6, DENND2A, PRCC, SRSF1, EIF4ENIF1, SPTAN1, CDCA8, PLS3, API5P1, API5, PSMA4, DNTTIP1, AKAP8, NCOA3, SMTN, FBRS, SMARCE1, ERC1, WBP11, SPTBN1, NFRKB, OLA1, ZNF207, R3HDM1, TRIM33, SAFB, UBFD1, SRSF7, SRSF2, GATA6, VDAC1P1, VDAC1, IL16, GMNN, ILF2, MED4, QKI, VCL, MFAP1, SNAP29, PADI1, BCL9, BCL9L, PKM, GPATCH11, CASC3, PSMC6, CACYBP, RPL12P6, RPL12P32, RPL12P14, RPL12, RPL12P2, RPL12P35, RPL12P19, TPI1, TPI1P1, CHAF1A, MIA3, CIC, SDCBP, CA2, FKBP3, ACE, NKX2-5, CSTF2, PFDN2, UBTF, FAM207A, LOC729774, BRD8, C12orf45, C1orf35, TCF20, SOD1, SPAG7, MED8, ETS2, ALDOC, FKBP4, INCENP, CEP85, CECR2, TFE3, SUPT16HP1, SUPT16H, MAPT, HTATSF1, RPS18P12, RPS18P5, KPNA2, TMX1, CKAP4, HSPE1, COPRS, PTGES3, LAMP2, ERLIN2, CTNNBL1, TOMM22, NRBF2, C9orf78, NCOA6, MED26, RANBP1, LOC389842, LOC727803, HMGB3, CANX, PUS7, RPSAP19, RPSA, RPSAP18, RPSAP58, RPSAP15, RPSAP8, RPSAP9, RPSAP12, RPSAP29, RPSAP61, ARHGAP17, USF1, PSIP1, SNRPEP2, SNRPE, NUDT5, PPM1G, OTUB1, AHCY, COPS3, NSMCE2, SAE1, PROSER1, GRWD1, CREB5, TAF9B, RBM33, EDF1, PGK1, FAM114A2, SRRM1, RAD23B, CIAPIN1, CIAPIN1P, LRRC59, PABPN1, KMT2A, RPRD1B, GPATCH8, CCDC43, DGCR14, PPP1R2, ERICH1, EIF5A, EIF5AL1, BAG3, PCNA, SOX7, PNISR, FAM168A, MED15, SRSF11, SIRT1, RSF1, MAML1, HPRT1, SPDL1, CRTC3, CEP55, CDV3, ALYREF, RNF40, STOML2, DGCR8, NUCKS1, UBN2, PSMD7, WNT10A, HMBS, KHDRBS1, VBP1, NCSTN, CDCA2, SFSWAP, ZRANB2, DDB1, RBBP6, ZEB1, SRSF6, LOC644422, EIF2S1, RFX5, RPS19, RPS19P3, TALDO1, CWC15, CDCA5, LOC645086, C11orf58, TXN, STX12, PHRF1, BSG, TAF4, SH3GL1, LIN37, HRNR, FAM192A, RRBP1, KIAA0907, GOLGB1, PAX9, P4HB, CHMP5, LDHB, CALR, SUMO2P1, SUMO2, PDIA6, AHSA1, EN2, CCDC124, RPLP0P6, RPLP0P2, RPLP0P3, RPLP0, NIPBL, PDLIM4, PRKCSH, C15orf39, HNRNPDL, PDIA4, NUP210, RPLP2P3, RPLP2, PRDX4, DAZAP1, UBE2T, PHAX, AMOT, MARCKS, LOC284685, SMARCC1, BCORL1, RFC4, GLRX3, ANP32E, HYOU1, NPM3, ATF7IP, SARNP, TRA2A, HDGF, STIP1, PELP1, KCTD12, GLO1, PCF11, CLIC1, DNAJC8, RNF114, SLC4A1AP, FAM50A, GTF2A1, PRPF40A, CDC37, PPIAP22, PPIA, SMARCC2, MEGF11, KIAA1143, DENR, LAMP1, MYBL2, PITX1, UBE2MP1, UBE2M, CHTF8, OTX1, NACA, FNBP4, GTF2F2, GLTSCR1, GTF2E1, PQBP1, EMD, RNF113A, GPALPP1, SNRPA, RRP15, RPS25, RPS25P8, GMEB2, LNPEP, DNAJB1, IGBP1P1, IGBP1, HINT1, ARID1A, PPIB, ANXA11, MATR3, Sgol2, PPARGC1A, MCM2, Ksr1, UBASH3B, SFPQ, CAPN13, HEY1, BRCA1, MTCH2, PPIE, TBX3, BMP4, CTNNB1, GSK3A, HNF1B, TCF7L2, TRIP4, YAF2, ZNF217, AAR2, PIH1D1, EFTUD2, TNIP2, CHD3, CHD4, HEXIM1, MEPCE, LARP7, RUNX1, AGR2, RECQL4, REST, CDK9, SMARCA4, DDIT3, FLI1, TP53BP1, MDC1, METTL3, METTL14, KIAA1429, RC3H1, RC3H2, ATG16L1, PHB, DISC1, NR2C2, UBQLN2, ZFYVE21, XRCC6, AGRN, USP19, HIST1H4A, APEX1, DDX5, SNRNP70, SNRPB, SNRPD1, SNRPD2, SNRPD3, RNU1-1, RBMX, HNRNPM, HNRNPA2B1, TAF15, DDX3X, TARDBP, CLINT1, HNRNPL, NUMA1, ZFR, SNRNP200, ZNF326, HNRNPK, SF3B1, TOE1, HSPA8, SNRPB2, DDX20, GOT2, ILF3, PRPF6, ZNF638, HNRNPF, HNRNPH1, HNRNPR, VCP, CAD, CCAR2, DDX23, GEMIN4, HSPA1A, PCMTD1, POTEF, PRMT5, RBM45, SAFB2, SF3B2, SF3B3, SNRPF, SRSF4, THRAP3, TIA1, TTC7A, ZCCHC8, ITFG1, ARAF, BRD7, SOX2, ARIH2, PLEKHA4, NGB, OPTN, ZC3H18, CELF1, MKI67, INS, Apc2, FBP1, N, ZNF768, SYNCRIP, KDM5C, DDX58, OGT, SPOP, UFL1, DDRGK1, WDR5, TPX2, MALL, SOX21, POU3F3, PTP4A3, TRIM8, RCHY1, nsp14, SOX5,

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
EWSR1
WT1

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

Top

Related Drugs to EWSR1-WT1

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

Top

Related Diseases to EWSR1-WT1

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene	Tgene	Disease	Source	PMID
EWSR1	WT1	Desmoplastic Small Round Cell Tumor	MyCancerGenome
EWSR1	WT1	Acute Myeloid Leukemia	MyCancerGenome
EWSR1	WT1	Astrocytoma	MyCancerGenome
EWSR1	WT1	Breast Invasive Ductal Carcinoma	MyCancerGenome
EWSR1	WT1	Central Nervous System Neoplasm	MyCancerGenome

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner	Gene	Disease ID	Disease name	# pubmeds	Source
Hgene	EWSR1	C0553580	Ewings sarcoma	3	CTD_human;ORPHANET
Hgene	EWSR1	C0002736	Amyotrophic Lateral Sclerosis	1	GENOMICS_ENGLAND
Hgene	EWSR1	C0033578	Prostatic Neoplasms	1	CTD_human
Hgene	EWSR1	C0206651	Clear Cell Sarcoma of Soft Tissue	1	ORPHANET
Hgene	EWSR1	C0206663	Neuroectodermal Tumor, Primitive	1	CTD_human
Hgene	EWSR1	C0279980	Extra-osseous Ewing's sarcoma	1	ORPHANET
Hgene	EWSR1	C0334584	Spongioblastoma	1	CTD_human
Hgene	EWSR1	C0334596	Medulloepithelioma	1	CTD_human
Hgene	EWSR1	C0376358	Malignant neoplasm of prostate	1	CTD_human
Hgene	EWSR1	C0700367	Ependymoblastoma	1	CTD_human
Hgene	EWSR1	C0751675	Cerebral Primitive Neuroectodermal Tumor	1	CTD_human
Hgene	EWSR1	C1275278	Extraskeletal Myxoid Chondrosarcoma	1	ORPHANET
Tgene	WT1	C0950121	Denys-Drash Syndrome	20	CTD_human;GENOMICS_ENGLAND;ORPHANET;UNIPROT
Tgene	WT1	C0027708	Nephroblastoma	8	CGI;CTD_human;GENOMICS_ENGLAND;ORPHANET;UNIPROT
Tgene	WT1	C3151568	NEPHROTIC SYNDROME, TYPE 4	6	CTD_human;GENOMICS_ENGLAND;UNIPROT
Tgene	WT1	C0950122	Frasier Syndrome	4	CTD_human;GENOMICS_ENGLAND;ORPHANET;UNIPROT
Tgene	WT1	C1837026	MEACHAM SYNDROME (disorder)	3	CTD_human;GENOMICS_ENGLAND;ORPHANET;UNIPROT
Tgene	WT1	C0023467	Leukemia, Myelocytic, Acute	2	CGI;CTD_human
Tgene	WT1	C0026998	Acute Myeloid Leukemia, M1	2	CTD_human
Tgene	WT1	C0345967	Malignant mesothelioma	2	CTD_human;GENOMICS_ENGLAND
Tgene	WT1	C1879321	Acute Myeloid Leukemia (AML-M2)	2	CTD_human
Tgene	WT1	C2930471	Bilateral Wilms Tumor	2	CTD_human
Tgene	WT1	C0006142	Malignant neoplasm of breast	1	CTD_human
Tgene	WT1	C0007102	Malignant tumor of colon	1	CTD_human
Tgene	WT1	C0009375	Colonic Neoplasms	1	CTD_human
Tgene	WT1	C0017636	Glioblastoma	1	CTD_human
Tgene	WT1	C0017658	Glomerulonephritis	1	CTD_human
Tgene	WT1	C0018054	Gonadal Dysgenesis, 46,XY	1	ORPHANET
Tgene	WT1	C0019284	Diaphragmatic Hernia	1	CTD_human
Tgene	WT1	C0023418	leukemia	1	CTD_human
Tgene	WT1	C0023473	Myeloid Leukemia, Chronic	1	CTD_human
Tgene	WT1	C0023487	Acute Promyelocytic Leukemia	1	CTD_human
Tgene	WT1	C0024121	Lung Neoplasms	1	CTD_human
Tgene	WT1	C0027809	Neurilemmoma	1	CTD_human
Tgene	WT1	C0029463	Osteosarcoma	1	CTD_human
Tgene	WT1	C0030297	Pancreatic Neoplasm	1	CTD_human
Tgene	WT1	C0031149	Peritoneal Neoplasms	1	CTD_human
Tgene	WT1	C0085215	Ovarian Failure, Premature	1	CTD_human
Tgene	WT1	C0086367	Gonadotropin-Resistant Ovary Syndrome	1	CTD_human
Tgene	WT1	C0206115	WAGR Syndrome	1	CTD_human;GENOMICS_ENGLAND;ORPHANET
Tgene	WT1	C0235833	Congenital diaphragmatic hernia	1	CTD_human
Tgene	WT1	C0242379	Malignant neoplasm of lung	1	CTD_human
Tgene	WT1	C0265699	Congenital hernia of foramen of Morgagni	1	CTD_human
Tgene	WT1	C0265700	Congenital hernia of foramen of Bochdalek	1	CTD_human
Tgene	WT1	C0334588	Giant Cell Glioblastoma	1	CTD_human
Tgene	WT1	C0346647	Malignant neoplasm of pancreas	1	CTD_human
Tgene	WT1	C0346990	Carcinomatosis of peritoneal cavity	1	CTD_human
Tgene	WT1	C0678222	Breast Carcinoma	1	CTD_human
Tgene	WT1	C0751374	Schwannomatosis, Plexiform	1	CTD_human
Tgene	WT1	C1257931	Mammary Neoplasms, Human	1	CTD_human
Tgene	WT1	C1458155	Mammary Neoplasms	1	CTD_human
Tgene	WT1	C1621958	Glioblastoma Multiforme	1	CTD_human
Tgene	WT1	C1704377	Bright Disease	1	CTD_human
Tgene	WT1	C1840452	Hyaloideoretinal degeneration of Wagner	1	GENOMICS_ENGLAND
Tgene	WT1	C1868672	NEPHROTIC SYNDROME, STEROID-RESISTANT, AUTOSOMAL RECESSIVE	1	ORPHANET
Tgene	WT1	C2931803	Deletion 11p13	1	ORPHANET
Tgene	WT1	C2936694	Swyer Syndrome	1	ORPHANET
Tgene	WT1	C3494522	Hypergonadotropic Ovarian Failure, X-Linked	1	CTD_human
Tgene	WT1	C4510744	46,XY partial gonadal dysgenesis	1	ORPHANET
Tgene	WT1	C4552079	Premature Ovarian Failure 1	1	CTD_human
Tgene	WT1	C4704874	Mammary Carcinoma, Human	1	CTD_human