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Fusion Protein:FOXO3-GMDS |
Fusion Protein Summary |
 Fusion gene summary |
| Fusion partner gene information | Fusion gene name: FOXO3-GMDS | FusionPDB ID: 31176 | FusionGDB2.0 ID: 31176 | Hgene | Tgene | Gene symbol | FOXO3 | GMDS | Gene ID | 2309 | 2762 |
| Gene name | forkhead box O3 | GDP-mannose 4,6-dehydratase | |
| Synonyms | AF6q21|FKHRL1|FKHRL1P2|FOXO2|FOXO3A | GMD|SDR3E1 | |
| Cytomap | 6q21 | 6p25.3 | |
| Type of gene | protein-coding | protein-coding | |
| Description | forkhead box protein O3forkhead box O3Aforkhead homolog (rhabdomyosarcoma) like 1forkhead in rhabdomyosarcoma-like 1forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1 | GDP-mannose 4,6 dehydrataseGDP-D-mannose dehydrataseshort chain dehydrogenase/reductase family 3E, member 1 | |
| Modification date | 20200329 | 20200313 | |
| UniProtAcc | O43524 | O60547 | |
| Ensembl transtripts involved in fusion gene | ENST ids | ENST00000343882, ENST00000406360, ENST00000540898, | ENST00000467288, ENST00000380815, ENST00000530927, |
| Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0) | * DoF score | 9 X 5 X 7=315 | 14 X 10 X 8=1120 |
| # samples | 12 | 15 | |
| ** MAII score | log2(12/315*10)=-1.39231742277876 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | log2(15/1120*10)=-2.90046432644909 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | |
| Context (manual curation of fusion genes in FusionPDB) | PubMed: FOXO3 [Title/Abstract] AND GMDS [Title/Abstract] AND fusion [Title/Abstract] | ||
| Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0) | FOXO3(108883032)-GMDS(1961200), # samples:2 | ||
| Anticipated loss of major functional domain due to fusion event. | FOXO3-GMDS seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. FOXO3-GMDS seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. | ||
| * DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
| Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
| Partner | Gene | GO ID | GO term | PubMed ID |
| Hgene | FOXO3 | GO:0000122 | negative regulation of transcription by RNA polymerase II | 20371612 |
| Hgene | FOXO3 | GO:0006417 | regulation of translation | 21329882 |
| Hgene | FOXO3 | GO:0043065 | positive regulation of apoptotic process | 20371612 |
| Hgene | FOXO3 | GO:0045648 | positive regulation of erythrocyte differentiation | 14734530 |
| Hgene | FOXO3 | GO:0045893 | positive regulation of transcription, DNA-templated | 10102273|15084260 |
| Hgene | FOXO3 | GO:0045944 | positive regulation of transcription by RNA polymerase II | 10102273|14734530 |
| Tgene | GMDS | GO:0019673 | GDP-mannose metabolic process | 9525924 |
| Tgene | GMDS | GO:0042351 | 'de novo' GDP-L-fucose biosynthetic process | 9525924 |
| Fusion gene breakpoints across FOXO3 (5'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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| Fusion gene breakpoints across GMDS (3'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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Fusion Gene Sample Information |
| Fusion gene information from FusionGDB2.0. |
| Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0) * All genome coordinats were lifted-over on hg19. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| Source | Disease | Sample | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| ChimerDB4 | COAD | TCGA-DM-A0XF-01A | FOXO3 | chr6 | 108883032 | + | GMDS | chr6 | 1961200 | - |
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Fusion ORF Analysis |
| Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
| ENST00000343882 | FOXO3 | chr6 | 108883032 | + | ENST00000530927 | GMDS | chr6 | 1961200 | - | 2062 | 925 | 238 | 1698 | 486 |
| ENST00000343882 | FOXO3 | chr6 | 108883032 | + | ENST00000380815 | GMDS | chr6 | 1961200 | - | 2062 | 925 | 238 | 1698 | 486 |
| ENST00000406360 | FOXO3 | chr6 | 108883032 | + | ENST00000530927 | GMDS | chr6 | 1961200 | - | 2101 | 964 | 205 | 1737 | 510 |
| ENST00000406360 | FOXO3 | chr6 | 108883032 | + | ENST00000380815 | GMDS | chr6 | 1961200 | - | 2101 | 964 | 205 | 1737 | 510 |
| DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
| ENST00000343882 | ENST00000530927 | FOXO3 | chr6 | 108883032 | + | GMDS | chr6 | 1961200 | - | 0.005954035 | 0.994046 |
| ENST00000343882 | ENST00000380815 | FOXO3 | chr6 | 108883032 | + | GMDS | chr6 | 1961200 | - | 0.005954035 | 0.994046 |
| ENST00000406360 | ENST00000530927 | FOXO3 | chr6 | 108883032 | + | GMDS | chr6 | 1961200 | - | 0.005406715 | 0.9945933 |
| ENST00000406360 | ENST00000380815 | FOXO3 | chr6 | 108883032 | + | GMDS | chr6 | 1961200 | - | 0.005406715 | 0.9945933 |
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Fusion Amino Acid Sequences |
| For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones. |
| >FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP >31176_31176_1_FOXO3-GMDS_FOXO3_chr6_108883032_ENST00000343882_GMDS_chr6_1961200_ENST00000380815_length(amino acids)=486AA_BP=229 MHPAPAREESARAPAAGGRAAKMAEAPASPAPLSPLEVELDPEFEPQSRPRSCTWPLQRPELQASPAKPSGETAADSMIPEEEDDEDDED GGGRAGSAMAIGGGGGSGTLGSGLLLEDSARVLAPGGQDPGSGPATAAGGLSGGTQALLQPQQPLPPPQPGAAGGSGQPRKCSSRRNAWG NLSYADLITRAIESSPDKRLTLSQIYEWMVRCVPYFKDKGDSNSSAGWKISFDLAEYTADVDGVGTLRLLDAVKTCGLINSVKFYQASTS ELYGKVQEIPQKETTPFYPRSPYGAAKLYAYWIVVNFREAYNLFAVNGILFNHESPRRGANFVTRKISRSVAKIYLGQLECFSLGNLDAK RDWGHAKDYVEAMWLMLQNDEPEDFVIATGEVHSVREFVEKSFLHIGKTIVWEGKNENEVGRCKETGKVHVTVDLKYYRPTEVDFLQGDC -------------------------------------------------------------- >31176_31176_2_FOXO3-GMDS_FOXO3_chr6_108883032_ENST00000343882_GMDS_chr6_1961200_ENST00000530927_length(amino acids)=486AA_BP=229 MHPAPAREESARAPAAGGRAAKMAEAPASPAPLSPLEVELDPEFEPQSRPRSCTWPLQRPELQASPAKPSGETAADSMIPEEEDDEDDED GGGRAGSAMAIGGGGGSGTLGSGLLLEDSARVLAPGGQDPGSGPATAAGGLSGGTQALLQPQQPLPPPQPGAAGGSGQPRKCSSRRNAWG NLSYADLITRAIESSPDKRLTLSQIYEWMVRCVPYFKDKGDSNSSAGWKISFDLAEYTADVDGVGTLRLLDAVKTCGLINSVKFYQASTS ELYGKVQEIPQKETTPFYPRSPYGAAKLYAYWIVVNFREAYNLFAVNGILFNHESPRRGANFVTRKISRSVAKIYLGQLECFSLGNLDAK RDWGHAKDYVEAMWLMLQNDEPEDFVIATGEVHSVREFVEKSFLHIGKTIVWEGKNENEVGRCKETGKVHVTVDLKYYRPTEVDFLQGDC -------------------------------------------------------------- >31176_31176_3_FOXO3-GMDS_FOXO3_chr6_108883032_ENST00000406360_GMDS_chr6_1961200_ENST00000380815_length(amino acids)=510AA_BP=253 MVLPQAAAAAPGSRSLRGVHVPPPLHPAPAREESARAPAAGGRAAKMAEAPASPAPLSPLEVELDPEFEPQSRPRSCTWPLQRPELQASP AKPSGETAADSMIPEEEDDEDDEDGGGRAGSAMAIGGGGGSGTLGSGLLLEDSARVLAPGGQDPGSGPATAAGGLSGGTQALLQPQQPLP PPQPGAAGGSGQPRKCSSRRNAWGNLSYADLITRAIESSPDKRLTLSQIYEWMVRCVPYFKDKGDSNSSAGWKISFDLAEYTADVDGVGT LRLLDAVKTCGLINSVKFYQASTSELYGKVQEIPQKETTPFYPRSPYGAAKLYAYWIVVNFREAYNLFAVNGILFNHESPRRGANFVTRK ISRSVAKIYLGQLECFSLGNLDAKRDWGHAKDYVEAMWLMLQNDEPEDFVIATGEVHSVREFVEKSFLHIGKTIVWEGKNENEVGRCKET -------------------------------------------------------------- >31176_31176_4_FOXO3-GMDS_FOXO3_chr6_108883032_ENST00000406360_GMDS_chr6_1961200_ENST00000530927_length(amino acids)=510AA_BP=253 MVLPQAAAAAPGSRSLRGVHVPPPLHPAPAREESARAPAAGGRAAKMAEAPASPAPLSPLEVELDPEFEPQSRPRSCTWPLQRPELQASP AKPSGETAADSMIPEEEDDEDDEDGGGRAGSAMAIGGGGGSGTLGSGLLLEDSARVLAPGGQDPGSGPATAAGGLSGGTQALLQPQQPLP PPQPGAAGGSGQPRKCSSRRNAWGNLSYADLITRAIESSPDKRLTLSQIYEWMVRCVPYFKDKGDSNSSAGWKISFDLAEYTADVDGVGT LRLLDAVKTCGLINSVKFYQASTSELYGKVQEIPQKETTPFYPRSPYGAAKLYAYWIVVNFREAYNLFAVNGILFNHESPRRGANFVTRK ISRSVAKIYLGQLECFSLGNLDAKRDWGHAKDYVEAMWLMLQNDEPEDFVIATGEVHSVREFVEKSFLHIGKTIVWEGKNENEVGRCKET -------------------------------------------------------------- |
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Fusion Protein Functional Features |
| Four levels of functional features of fusion genes Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr6:108883032/chr6:1961200) - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. - How to search 1. Put your fusion gene symbol. 2. Press the tab key until there will be shown the breakpoint information filled. 4. Go down and press 'Search' tab twice. 4. Go down to have the hyperlink of the search result. 5. Click the hyperlink. 6. See the FGviewer result for your fusion gene. |
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| Main function of each fusion partner protein. (from UniProt) |
| Hgene | Tgene |
| FOXO3 | GMDS |
| FUNCTION: Transcriptional activator that recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3' and regulates different processes, such as apoptosis and autophagy (PubMed:10102273, PubMed:16751106, PubMed:21329882). Acts as a positive regulator of autophagy in skeletal muscle: in starved cells, enters the nucleus following dephosphorylation and binds the promoters of autophagy genes, such as GABARAP1L, MAP1LC3B and ATG12, thereby activating their expression, resulting in proteolysis of skeletal muscle proteins (By similarity). Triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress (PubMed:10102273, PubMed:16751106). Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation (PubMed:21329882). In response to metabolic stress, translocates into the mitochondria where it promotes mtDNA transcription (PubMed:23283301). In response to metabolic stress, translocates into the mitochondria where it promotes mtDNA transcription. Also acts as a key regulator of chondrogenic commitment of skeletal progenitor cells in response to lipid availability: when lipids levels are low, translocates to the nucleus and promotes expression of SOX9, which induces chondrogenic commitment and suppresses fatty acid oxidation (By similarity). {ECO:0000250|UniProtKB:Q9WVH4, ECO:0000269|PubMed:10102273, ECO:0000269|PubMed:16751106, ECO:0000269|PubMed:21329882, ECO:0000269|PubMed:23283301}. | FUNCTION: Catalyzes the conversion of GDP-D-mannose to GDP-4-dehydro-6-deoxy-D-mannose. {ECO:0000269|PubMed:9525924}. |
| Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at * Minus value of BPloci means that the break pointn is located before the CDS. |
| - Retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| Tgene | GMDS | chr6:108883032 | chr6:1961200 | ENST00000530927 | 3 | 11 | 108_112 | 85.0 | 343.0 | Nucleotide binding | NADP | |
| Tgene | GMDS | chr6:108883032 | chr6:1961200 | ENST00000530927 | 3 | 11 | 86_87 | 85.0 | 343.0 | Nucleotide binding | NADP |
| - Not-retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| Hgene | FOXO3 | chr6:108883032 | chr6:1961200 | ENST00000343882 | + | 2 | 4 | 157_251 | 207.0 | 2263.0 | DNA binding | Fork-head |
| Hgene | FOXO3 | chr6:108883032 | chr6:1961200 | ENST00000406360 | + | 1 | 3 | 157_251 | 207.0 | 2000.0 | DNA binding | Fork-head |
| Hgene | FOXO3 | chr6:108883032 | chr6:1961200 | ENST00000343882 | + | 2 | 4 | 242_259 | 207.0 | 2263.0 | Motif | Nuclear localization signal |
| Hgene | FOXO3 | chr6:108883032 | chr6:1961200 | ENST00000406360 | + | 1 | 3 | 242_259 | 207.0 | 2000.0 | Motif | Nuclear localization signal |
| Tgene | GMDS | chr6:108883032 | chr6:1961200 | ENST00000380815 | 3 | 11 | 108_112 | 115.0 | 373.0 | Nucleotide binding | NADP | |
| Tgene | GMDS | chr6:108883032 | chr6:1961200 | ENST00000380815 | 3 | 11 | 30_35 | 115.0 | 373.0 | Nucleotide binding | NADP | |
| Tgene | GMDS | chr6:108883032 | chr6:1961200 | ENST00000380815 | 3 | 11 | 55_58 | 115.0 | 373.0 | Nucleotide binding | NADP | |
| Tgene | GMDS | chr6:108883032 | chr6:1961200 | ENST00000380815 | 3 | 11 | 86_87 | 115.0 | 373.0 | Nucleotide binding | NADP | |
| Tgene | GMDS | chr6:108883032 | chr6:1961200 | ENST00000530927 | 3 | 11 | 30_35 | 85.0 | 343.0 | Nucleotide binding | NADP | |
| Tgene | GMDS | chr6:108883032 | chr6:1961200 | ENST00000530927 | 3 | 11 | 55_58 | 85.0 | 343.0 | Nucleotide binding | NADP |
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Fusion Protein-Protein Interaction |
| Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in |
| Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160) |
| Gene | PPI interactors |
| GMDS | GMDS, TNKS, BLVRB, BAG3, ATIC, CAPN2, CTH, NSFL1C, PTMA, RAP1GDS1, TATDN1, WDYHV1, RNF166, MAT2B, RAB2A, TTC5, NDUFA9, PSMG4, CCDC22, SKA1, Ddb1, Kif2a, Smad3, Rab5c, Tnks, Shoc2, Tnks2, NF2, FBXW7, TNKS2, HIF1AN, NPHP3, ORF17.5, TAE1, TRIM14, EGLN3, EFTUD2, AAR2, PIH1D1, RBX1, BMH2, BMH1, KCNAB3, PLEKHA4, HSPA1B, HSPA8, PAGE4, PPP1R12B, CHD1L, SPRTN, |
| Protein-protein interactors based on sequence similarity (STRING) |
| Gene | STRING network |
| FOXO3 | |
| GMDS |  |
| - Retained interactions in fusion protein (protein functional feature from UniProt). |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Still interaction with |
| - Lost interactions due to fusion (protein functional feature from UniProt). |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
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Related Drugs to FOXO3-GMDS |
| Drugs used for this fusion-positive patient. (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
| Hgene | Tgene | Drug | Source | PMID |
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Related Diseases to FOXO3-GMDS |
| Diseases that have this fusion gene. (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
| Hgene | Tgene | Disease | Source | PMID |
| Diseases associated with fusion partners. (DisGeNet 4.0) |
| Partner | Gene | Disease ID | Disease name | # pubmeds | Source |
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