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	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:GNA11-TLE6

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: GNA11-TLE6
	FusionPDB ID: 33521
	FusionGDB2.0 ID: 33521
		Hgene	Tgene
	Gene symbol	GNA11	TLE6
	Gene ID	2767	79816
	Gene name	G protein subunit alpha 11	TLE family member 6, subcortical maternal complex member
	Synonyms	FBH\|FBH2\|FHH2\|GNA-11\|HHC2\|HYPOC2	GRG6\|PREMBL
	Cytomap	19p13.3	19p13.3
	Type of gene	protein-coding	protein-coding
	Description	guanine nucleotide-binding protein subunit alpha-11g alpha-11guanine nucleotide binding protein (G protein), alpha 11 (Gq class)guanine nucleotide-binding protein G(y) subunit alpha	transducin-like enhancer protein 6transducin like enhancer of split 6transducin-like enhancer of split 6 (E(sp1) homolog, Drosophila)
	Modification date	20200313	20200313
	UniProtAcc	P29992	.
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000078429, ENST00000586180,	ENST00000452088, ENST00000478073, ENST00000482627, ENST00000246112,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	8 X 4 X 5=160	5 X 5 X 5=125
	# samples	12	7
	** MAII score	log2(12/160*10)=-0.415037499278844 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(7/125*10)=-0.836501267717121 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)	PubMed: GNA11 [Title/Abstract] AND TLE6 [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	GNA11(3113482)-TLE6(2991841), # samples:1
Anticipated loss of major functional domain due to fusion event.	GNA11-TLE6 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. GNA11-TLE6 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. GNA11-TLE6 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. GNA11-TLE6 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner

Gene

GO ID

GO term

PubMed ID

Fusion gene breakpoints across GNA11 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across TLE6 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	STAD	TCGA-BR-8295-01A	GNA11	chr19	3113482	+	TLE6	chr19	2991841	+

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst

Tenst

Hgene

Hchr

Hbp

Hstrand

Tgene

Tchr

Tbp

Tstrand

Seq length
(transcript)

BP loci
(transcript)

Predicted start
(transcript)

Predicted stop
(transcript)

Seq length
(amino acids)

ENST00000078429

GNA11

chr19

3113482

ENST00000246112

TLE6

chr19

2991841

1368

718

1180

393

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000078429	ENST00000246112	GNA11	chr19	3113482	+	TLE6	chr19	2991841	+	0.00279162	0.9972084

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>33521_33521_1_GNA11-TLE6_GNA11_chr19_3113482_ENST00000078429_TLE6_chr19_2991841_ENST00000246112_length(amino acids)=393AA_BP=218
MVHGGVLPGACDNEAVVGRDVTARDWRHLRHLEHFCPRRHAVDAKEVVHSNACPPLAKGGELDAQDDAVFLPHHVPLAPAGALQPLLAIG
HAQQHPVLLGAVGQAHYLRLELVLQRFHDGPLVPAPQTGIRTPSPDVVALDHDTLDSIRITLEVLLGRVGELVLAPAVVAFLDARVLPQG
LDGTDVLMLEGGHLLHVHLPDQERIGLVLLVLVEDLERLHGPDHGLHGGEDVLVDELGEAALVLLRVAGAVDDAHLLDERALPALARAEQ
QQLELAPGVALVPPQLLLDLGVDPLGLLHLIAQATRHHGLQSHRPGPGRRGRLPAAAPRPPARPWPEPPRPPPAAALRPRPPALGPGPAR

--------------------------------------------------------------

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Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr19:3113482/chr19:2991841)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
GNA11 P29992	.
FUNCTION: Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Acts as an activator of phospholipase C. Transduces FFAR4 signaling in response to long-chain fatty acids (LCFAs). {ECO:0000269\|PubMed:27852822}.	FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

GNA11

chr19:3113482

chr19:2991841

ENST00000078429

46_53

158.66666666666666

360.0

Nucleotide binding

GTP

Hgene

GNA11

chr19:3113482

chr19:2991841

ENST00000078429

41_54

158.66666666666666

360.0

Region

G1 motif

Tgene

TLE6

chr19:3113482

chr19:2991841

ENST00000246112

419_456

414.6666666666667

573.0

Repeat

Note=WD 4

Tgene

TLE6

chr19:3113482

chr19:2991841

ENST00000246112

458_497

414.6666666666667

573.0

Repeat

Note=WD 5

Tgene

TLE6

chr19:3113482

chr19:2991841

ENST00000246112

499_538

414.6666666666667

573.0

Repeat

Note=WD 6

Tgene

TLE6

chr19:3113482

chr19:2991841

ENST00000246112

540_571

414.6666666666667

573.0

Repeat

Note=WD 7

Tgene

TLE6

chr19:3113482

chr19:2991841

ENST00000452088

332_372

291.6666666666667

450.0

Repeat

Note=WD 2

Tgene

TLE6

chr19:3113482

chr19:2991841

ENST00000452088

377_416

291.6666666666667

450.0

Repeat

Note=WD 3

Tgene

TLE6

chr19:3113482

chr19:2991841

ENST00000452088

419_456

291.6666666666667

450.0

Repeat

Note=WD 4

Tgene

TLE6

chr19:3113482

chr19:2991841

ENST00000452088

458_497

291.6666666666667

450.0

Repeat

Note=WD 5

Tgene

TLE6

chr19:3113482

chr19:2991841

ENST00000452088

499_538

291.6666666666667

450.0

Repeat

Note=WD 6

Tgene

TLE6

chr19:3113482

chr19:2991841

ENST00000452088

540_571

291.6666666666667

450.0

Repeat

Note=WD 7

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

GNA11

chr19:3113482

chr19:2991841

ENST00000078429

38_359

158.66666666666666

360.0

Domain

G-alpha

Hgene

GNA11

chr19:3113482

chr19:2991841

ENST00000078429

180_186

158.66666666666666

360.0

Nucleotide binding

GTP

Hgene

GNA11

chr19:3113482

chr19:2991841

ENST00000078429

205_209

158.66666666666666

360.0

Nucleotide binding

GTP

Hgene

GNA11

chr19:3113482

chr19:2991841

ENST00000078429

274_277

158.66666666666666

360.0

Nucleotide binding

GTP

Hgene

GNA11

chr19:3113482

chr19:2991841

ENST00000078429

178_186

158.66666666666666

360.0

Region

G2 motif

Hgene

GNA11

chr19:3113482

chr19:2991841

ENST00000078429

201_210

158.66666666666666

360.0

Region

G3 motif

Hgene

GNA11

chr19:3113482

chr19:2991841

ENST00000078429

270_277

158.66666666666666

360.0

Region

G4 motif

Hgene

GNA11

chr19:3113482

chr19:2991841

ENST00000078429

329_334

158.66666666666666

360.0

Region

G5 motif

Tgene

TLE6

chr19:3113482

chr19:2991841

ENST00000246112

284_322

414.6666666666667

573.0

Repeat

Note=WD 1

Tgene

TLE6

chr19:3113482

chr19:2991841

ENST00000246112

332_372

414.6666666666667

573.0

Repeat

Note=WD 2

Tgene

TLE6

chr19:3113482

chr19:2991841

ENST00000246112

377_416

414.6666666666667

573.0

Repeat

Note=WD 3

Tgene

TLE6

chr19:3113482

chr19:2991841

ENST00000452088

284_322

291.6666666666667

450.0

Repeat

Note=WD 1

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Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
GNA11
TLE6

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

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Related Drugs to GNA11-TLE6

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

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Related Diseases to GNA11-TLE6

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source