UTHEALTH HOME ABOUT SBMI A-Z WEBMAIL INSIDE THE UNIVERSITY

Home

Download

Statistics

Examples

Help

Contact

level2

	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein Structure
	pLDDT scores
	Ramachandran Plot of Fusion Protein Structure
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:HBS1L-POLN

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: HBS1L-POLN
	FusionPDB ID: 35722
	FusionGDB2.0 ID: 35722
		Hgene	Tgene
	Gene symbol	HBS1L	POLN
	Gene ID	10767	353497
	Gene name	HBS1 like translational GTPase	DNA polymerase nu
	Synonyms	EF-1a\|ERFS\|HBS1\|HSPC276\|eRF3c	POL4P
	Cytomap	6q23.3	4p16.3
	Type of gene	protein-coding	protein-coding
	Description	HBS1-like proteinERF3-similar proteinHsp70 subfamily B suppressor 1-like proteineRF3 family member	DNA polymerase nuDNA polymerase NDNA polymerase POL4Ppolymerase (DNA directed) nupolymerase (DNA) nu
	Modification date	20200313	20200313
	UniProtAcc	Q9Y450	.
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000314674, ENST00000367820, ENST00000367822, ENST00000367826, ENST00000367837, ENST00000525067, ENST00000367824, ENST00000415177, ENST00000445176, ENST00000527578,	ENST00000515357, ENST00000382865, ENST00000511885,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	6 X 5 X 6=180	17 X 13 X 10=2210
	# samples	7	21
	** MAII score	log2(7/180*10)=-1.36257007938471 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(21/2210*10)=-3.39558513661267 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)	PubMed: HBS1L [Title/Abstract] AND POLN [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	HBS1L(135360710)-POLN(2087471), # samples:2
Anticipated loss of major functional domain due to fusion event.	HBS1L-POLN seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. HBS1L-POLN seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Tgene	POLN	GO:0019985	translesion synthesis	20102227

Fusion gene breakpoints across HBS1L (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across POLN (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Top

Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	LIHC	TCGA-CC-A7IH	HBS1L	chr6	135360710	-	POLN	chr4	2087471	-
ChimerDB4	LIHC	TCGA-EP-A26S	HBS1L	chr6	135360710	-	POLN	chr4	2087471	-

Top

Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000367837	HBS1L	chr6	135360710	-	ENST00000382865	POLN	chr4	2087471	-	1471	637	207	1274	355
ENST00000367837	HBS1L	chr6	135360710	-	ENST00000511885	POLN	chr4	2087471	-	1471	637	207	1274	355
ENST00000367826	HBS1L	chr6	135360710	-	ENST00000382865	POLN	chr4	2087471	-	1266	432	128	1069	313
ENST00000367826	HBS1L	chr6	135360710	-	ENST00000511885	POLN	chr4	2087471	-	1266	432	128	1069	313
ENST00000314674	HBS1L	chr6	135360710	-	ENST00000382865	POLN	chr4	2087471	-	1376	542	112	1179	355
ENST00000314674	HBS1L	chr6	135360710	-	ENST00000511885	POLN	chr4	2087471	-	1376	542	112	1179	355
ENST00000367822	HBS1L	chr6	135360710	-	ENST00000382865	POLN	chr4	2087471	-	1457	623	193	1260	355
ENST00000367822	HBS1L	chr6	135360710	-	ENST00000511885	POLN	chr4	2087471	-	1457	623	193	1260	355
ENST00000367820	HBS1L	chr6	135360710	-	ENST00000382865	POLN	chr4	2087471	-	1373	539	109	1176	355
ENST00000367820	HBS1L	chr6	135360710	-	ENST00000511885	POLN	chr4	2087471	-	1373	539	109	1176	355
ENST00000525067	HBS1L	chr6	135360710	-	ENST00000382865	POLN	chr4	2087471	-	1233	399	161	1036	291
ENST00000525067	HBS1L	chr6	135360710	-	ENST00000511885	POLN	chr4	2087471	-	1233	399	161	1036	291

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000367837	ENST00000382865	HBS1L	chr6	135360710	-	POLN	chr4	2087471	-	0.008872327	0.99112767
ENST00000367837	ENST00000511885	HBS1L	chr6	135360710	-	POLN	chr4	2087471	-	0.008872327	0.99112767
ENST00000367826	ENST00000382865	HBS1L	chr6	135360710	-	POLN	chr4	2087471	-	0.005785403	0.9942146
ENST00000367826	ENST00000511885	HBS1L	chr6	135360710	-	POLN	chr4	2087471	-	0.005785403	0.9942146
ENST00000314674	ENST00000382865	HBS1L	chr6	135360710	-	POLN	chr4	2087471	-	0.007294476	0.9927055
ENST00000314674	ENST00000511885	HBS1L	chr6	135360710	-	POLN	chr4	2087471	-	0.007294476	0.9927055
ENST00000367822	ENST00000382865	HBS1L	chr6	135360710	-	POLN	chr4	2087471	-	0.008684593	0.99131536
ENST00000367822	ENST00000511885	HBS1L	chr6	135360710	-	POLN	chr4	2087471	-	0.008684593	0.99131536
ENST00000367820	ENST00000382865	HBS1L	chr6	135360710	-	POLN	chr4	2087471	-	0.007207796	0.99279225
ENST00000367820	ENST00000511885	HBS1L	chr6	135360710	-	POLN	chr4	2087471	-	0.007207796	0.99279225
ENST00000525067	ENST00000382865	HBS1L	chr6	135360710	-	POLN	chr4	2087471	-	0.020053796	0.9799462
ENST00000525067	ENST00000511885	HBS1L	chr6	135360710	-	POLN	chr4	2087471	-	0.020053796	0.9799462

Top

Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>35722_35722_1_HBS1L-POLN_HBS1L_chr6_135360710_ENST00000314674_POLN_chr4_2087471_ENST00000382865_length(amino acids)=355AA_BP=0
MARHRNVRGYNYDEDFEDDDLYGQSVEDDYCISPSTAAQFIYSRRDKPSVEPVEEYDYEDLKESSNSVSNHQLSGFDQARLYSCLDHMRE
VLGDAVPDEILIEAVLKNKFDVQKALSGVLEQDRVQSLKDKNEATVSTGKIAKGKERLAACLGVPIQEAAQFLESFLQKYKKIKDFARAA
IAQCHQTGCVVSIMGRRRPLPRIHAHDQQLRAQAERQAVNFVVQGSAADLCKLAMIHVFTAVAASHTLTARLVAQIHDELLFEVEDPQIP

--------------------------------------------------------------

>35722_35722_2_HBS1L-POLN_HBS1L_chr6_135360710_ENST00000314674_POLN_chr4_2087471_ENST00000511885_length(amino acids)=355AA_BP=0
MARHRNVRGYNYDEDFEDDDLYGQSVEDDYCISPSTAAQFIYSRRDKPSVEPVEEYDYEDLKESSNSVSNHQLSGFDQARLYSCLDHMRE
VLGDAVPDEILIEAVLKNKFDVQKALSGVLEQDRVQSLKDKNEATVSTGKIAKGKERLAACLGVPIQEAAQFLESFLQKYKKIKDFARAA
IAQCHQTGCVVSIMGRRRPLPRIHAHDQQLRAQAERQAVNFVVQGSAADLCKLAMIHVFTAVAASHTLTARLVAQIHDELLFEVEDPQIP

--------------------------------------------------------------

>35722_35722_3_HBS1L-POLN_HBS1L_chr6_135360710_ENST00000367820_POLN_chr4_2087471_ENST00000382865_length(amino acids)=355AA_BP=0
MARHRNVRGYNYDEDFEDDDLYGQSVEDDYCISPSTAAQFIYSRRDKPSVEPVEEYDYEDLKESSNSVSNHQLSGFDQARLYSCLDHMRE
VLGDAVPDEILIEAVLKNKFDVQKALSGVLEQDRVQSLKDKNEATVSTGKIAKGKERLAACLGVPIQEAAQFLESFLQKYKKIKDFARAA
IAQCHQTGCVVSIMGRRRPLPRIHAHDQQLRAQAERQAVNFVVQGSAADLCKLAMIHVFTAVAASHTLTARLVAQIHDELLFEVEDPQIP

--------------------------------------------------------------

>35722_35722_4_HBS1L-POLN_HBS1L_chr6_135360710_ENST00000367820_POLN_chr4_2087471_ENST00000511885_length(amino acids)=355AA_BP=0
MARHRNVRGYNYDEDFEDDDLYGQSVEDDYCISPSTAAQFIYSRRDKPSVEPVEEYDYEDLKESSNSVSNHQLSGFDQARLYSCLDHMRE
VLGDAVPDEILIEAVLKNKFDVQKALSGVLEQDRVQSLKDKNEATVSTGKIAKGKERLAACLGVPIQEAAQFLESFLQKYKKIKDFARAA
IAQCHQTGCVVSIMGRRRPLPRIHAHDQQLRAQAERQAVNFVVQGSAADLCKLAMIHVFTAVAASHTLTARLVAQIHDELLFEVEDPQIP

--------------------------------------------------------------

>35722_35722_5_HBS1L-POLN_HBS1L_chr6_135360710_ENST00000367822_POLN_chr4_2087471_ENST00000382865_length(amino acids)=355AA_BP=0
MARHRNVRGYNYDEDFEDDDLYGQSVEDDYCISPSTAAQFIYSRRDKPSVEPVEEYDYEDLKESSNSVSNHQLSGFDQARLYSCLDHMRE
VLGDAVPDEILIEAVLKNKFDVQKALSGVLEQDRVQSLKDKNEATVSTGKIAKGKERLAACLGVPIQEAAQFLESFLQKYKKIKDFARAA
IAQCHQTGCVVSIMGRRRPLPRIHAHDQQLRAQAERQAVNFVVQGSAADLCKLAMIHVFTAVAASHTLTARLVAQIHDELLFEVEDPQIP

--------------------------------------------------------------

>35722_35722_6_HBS1L-POLN_HBS1L_chr6_135360710_ENST00000367822_POLN_chr4_2087471_ENST00000511885_length(amino acids)=355AA_BP=0
MARHRNVRGYNYDEDFEDDDLYGQSVEDDYCISPSTAAQFIYSRRDKPSVEPVEEYDYEDLKESSNSVSNHQLSGFDQARLYSCLDHMRE
VLGDAVPDEILIEAVLKNKFDVQKALSGVLEQDRVQSLKDKNEATVSTGKIAKGKERLAACLGVPIQEAAQFLESFLQKYKKIKDFARAA
IAQCHQTGCVVSIMGRRRPLPRIHAHDQQLRAQAERQAVNFVVQGSAADLCKLAMIHVFTAVAASHTLTARLVAQIHDELLFEVEDPQIP

--------------------------------------------------------------

>35722_35722_7_HBS1L-POLN_HBS1L_chr6_135360710_ENST00000367826_POLN_chr4_2087471_ENST00000382865_length(amino acids)=313AA_BP=36
MARHRNVRGYNYDEDFEDDDLYGQSVEDDYCISPSTARLYSCLDHMREVLGDAVPDEILIEAVLKNKFDVQKALSGVLEQDRVQSLKDKN
EATVSTGKIAKGKERLAACLGVPIQEAAQFLESFLQKYKKIKDFARAAIAQCHQTGCVVSIMGRRRPLPRIHAHDQQLRAQAERQAVNFV
VQGSAADLCKLAMIHVFTAVAASHTLTARLVAQIHDELLFEVEDPQIPECAALVRRTMESLEQVQALELQLQVPLKVSLSAGRSWGHLVP

--------------------------------------------------------------

>35722_35722_8_HBS1L-POLN_HBS1L_chr6_135360710_ENST00000367826_POLN_chr4_2087471_ENST00000511885_length(amino acids)=313AA_BP=36
MARHRNVRGYNYDEDFEDDDLYGQSVEDDYCISPSTARLYSCLDHMREVLGDAVPDEILIEAVLKNKFDVQKALSGVLEQDRVQSLKDKN
EATVSTGKIAKGKERLAACLGVPIQEAAQFLESFLQKYKKIKDFARAAIAQCHQTGCVVSIMGRRRPLPRIHAHDQQLRAQAERQAVNFV
VQGSAADLCKLAMIHVFTAVAASHTLTARLVAQIHDELLFEVEDPQIPECAALVRRTMESLEQVQALELQLQVPLKVSLSAGRSWGHLVP

--------------------------------------------------------------

>35722_35722_9_HBS1L-POLN_HBS1L_chr6_135360710_ENST00000367837_POLN_chr4_2087471_ENST00000382865_length(amino acids)=355AA_BP=0
MARHRNVRGYNYDEDFEDDDLYGQSVEDDYCISPSTAAQFIYSRRDKPSVEPVEEYDYEDLKESSNSVSNHQLSGFDQARLYSCLDHMRE
VLGDAVPDEILIEAVLKNKFDVQKALSGVLEQDRVQSLKDKNEATVSTGKIAKGKERLAACLGVPIQEAAQFLESFLQKYKKIKDFARAA
IAQCHQTGCVVSIMGRRRPLPRIHAHDQQLRAQAERQAVNFVVQGSAADLCKLAMIHVFTAVAASHTLTARLVAQIHDELLFEVEDPQIP

--------------------------------------------------------------

>35722_35722_10_HBS1L-POLN_HBS1L_chr6_135360710_ENST00000367837_POLN_chr4_2087471_ENST00000511885_length(amino acids)=355AA_BP=0
MARHRNVRGYNYDEDFEDDDLYGQSVEDDYCISPSTAAQFIYSRRDKPSVEPVEEYDYEDLKESSNSVSNHQLSGFDQARLYSCLDHMRE
VLGDAVPDEILIEAVLKNKFDVQKALSGVLEQDRVQSLKDKNEATVSTGKIAKGKERLAACLGVPIQEAAQFLESFLQKYKKIKDFARAA
IAQCHQTGCVVSIMGRRRPLPRIHAHDQQLRAQAERQAVNFVVQGSAADLCKLAMIHVFTAVAASHTLTARLVAQIHDELLFEVEDPQIP

--------------------------------------------------------------

>35722_35722_11_HBS1L-POLN_HBS1L_chr6_135360710_ENST00000525067_POLN_chr4_2087471_ENST00000382865_length(amino acids)=291AA_BP=14
MARHRNVRGYNYDEARLYSCLDHMREVLGDAVPDEILIEAVLKNKFDVQKALSGVLEQDRVQSLKDKNEATVSTGKIAKGKERLAACLGV
PIQEAAQFLESFLQKYKKIKDFARAAIAQCHQTGCVVSIMGRRRPLPRIHAHDQQLRAQAERQAVNFVVQGSAADLCKLAMIHVFTAVAA
SHTLTARLVAQIHDELLFEVEDPQIPECAALVRRTMESLEQVQALELQLQVPLKVSLSAGRSWGHLVPLQEAWGPPPGPCRTESPSNSLA

--------------------------------------------------------------

>35722_35722_12_HBS1L-POLN_HBS1L_chr6_135360710_ENST00000525067_POLN_chr4_2087471_ENST00000511885_length(amino acids)=291AA_BP=14
MARHRNVRGYNYDEARLYSCLDHMREVLGDAVPDEILIEAVLKNKFDVQKALSGVLEQDRVQSLKDKNEATVSTGKIAKGKERLAACLGV
PIQEAAQFLESFLQKYKKIKDFARAAIAQCHQTGCVVSIMGRRRPLPRIHAHDQQLRAQAERQAVNFVVQGSAADLCKLAMIHVFTAVAA
SHTLTARLVAQIHDELLFEVEDPQIPECAALVRRTMESLEQVQALELQLQVPLKVSLSAGRSWGHLVPLQEAWGPPPGPCRTESPSNSLA

--------------------------------------------------------------

Top

Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr6:135360710/chr4:2087471)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
HBS1L Q9Y450	.
FUNCTION: Cotranslational quality control factor involved in the No-Go Decay (NGD) pathway (PubMed:21448132). In the presence of ABCE1 and PELO, is required for 48S complex formation from 80S ribosomes and dissociation of vacant 80S ribosomes (PubMed:21448132). Together with PELO and in presence of ABCE1, recognizes stalled ribosomes and promotes dissociation of elongation complexes assembled on non-stop mRNAs; this triggers endonucleolytic cleavage of the mRNA, a mechanism to release non-functional ribosomes and to degrade damaged mRNAs as part of the No-Go Decay (NGD) pathway (PubMed:21448132). {ECO:0000269\|PubMed:21448132}.; FUNCTION: [Isoform 2]: Facilitates the association of the exosome complex with the SKI complex. {ECO:0000269\|PubMed:28204585}.	FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

HBS1L

chr6:135360710

chr4:2087471

ENST00000367822

258_482

143.33333333333334

633.0

Domain

tr-type G

Hgene

HBS1L

chr6:135360710

chr4:2087471

ENST00000367826

258_482

101.33333333333333

643.0

Domain

tr-type G

Hgene

HBS1L

chr6:135360710

chr4:2087471

ENST00000367837

258_482

143.33333333333334

685.0

Domain

tr-type G

Hgene

HBS1L

chr6:135360710

chr4:2087471

ENST00000367822

344_348

143.33333333333334

633.0

Nucleotide binding

GTP

Hgene

HBS1L

chr6:135360710

chr4:2087471

ENST00000367826

344_348

101.33333333333333

643.0

Nucleotide binding

GTP

Hgene

HBS1L

chr6:135360710

chr4:2087471

ENST00000367837

344_348

143.33333333333334

685.0

Nucleotide binding

GTP

Hgene

HBS1L

chr6:135360710

chr4:2087471

ENST00000367822

267_274

143.33333333333334

633.0

Region

Hgene

HBS1L

chr6:135360710

chr4:2087471

ENST00000367822

323_327

143.33333333333334

633.0

Region

Hgene

HBS1L

chr6:135360710

chr4:2087471

ENST00000367822

344_347

143.33333333333334

633.0

Region

Hgene

HBS1L

chr6:135360710

chr4:2087471

ENST00000367822

406_409

143.33333333333334

633.0

Region

Hgene

HBS1L

chr6:135360710

chr4:2087471

ENST00000367822

445_447

143.33333333333334

633.0

Region

Hgene

HBS1L

chr6:135360710

chr4:2087471

ENST00000367826

267_274

101.33333333333333

643.0

Region

Hgene

HBS1L

chr6:135360710

chr4:2087471

ENST00000367826

323_327

101.33333333333333

643.0

Region

Hgene

HBS1L

chr6:135360710

chr4:2087471

ENST00000367826

344_347

101.33333333333333

643.0

Region

Hgene

HBS1L

chr6:135360710

chr4:2087471

ENST00000367826

406_409

101.33333333333333

643.0

Region

Hgene

HBS1L

chr6:135360710

chr4:2087471

ENST00000367826

445_447

101.33333333333333

643.0

Region

Hgene

HBS1L

chr6:135360710

chr4:2087471

ENST00000367837

267_274

143.33333333333334

685.0

Region

Hgene

HBS1L

chr6:135360710

chr4:2087471

ENST00000367837

323_327

143.33333333333334

685.0

Region

Hgene

HBS1L

chr6:135360710

chr4:2087471

ENST00000367837

344_347

143.33333333333334

685.0

Region

Hgene

HBS1L

chr6:135360710

chr4:2087471

ENST00000367837

406_409

143.33333333333334

685.0

Region

Hgene

HBS1L

chr6:135360710

chr4:2087471

ENST00000367837

445_447

143.33333333333334

685.0

Region

Top

Fusion Protein Structures

PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.

Fusion protein PDB link (fusion AA seq ID in FusionPDB)	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	AA seq	Len(AA seq)
PDB file >>>693_HBS1L_135360710_POLN_2087471_ranked_0.pdb	HBS1L	135360710	135360710	ENST00000511885	POLN	chr4	2087471	-	MARHRNVRGYNYDEDFEDDDLYGQSVEDDYCISPSTAAQFIYSRRDKPSVEPVEEYDYEDLKESSNSVSNHQLSGFDQARLYSCLDHMRE VLGDAVPDEILIEAVLKNKFDVQKALSGVLEQDRVQSLKDKNEATVSTGKIAKGKERLAACLGVPIQEAAQFLESFLQKYKKIKDFARAA IAQCHQTGCVVSIMGRRRPLPRIHAHDQQLRAQAERQAVNFVVQGSAADLCKLAMIHVFTAVAASHTLTARLVAQIHDELLFEVEDPQIP	355

Top

pLDDT score distribution

pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

HBS1L_pLDDT.png

POLN_pLDDT.png

pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

Top

Ramachandran Plot of Fusion Protein Structure

Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.

Fusion AA seq ID in FusionPDB and their Ramachandran plots

Top

Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
HBS1L
POLN

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

Top

Related Drugs to HBS1L-POLN

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

Top

Related Diseases to HBS1L-POLN

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source