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Center for Computational Systems Medicine level3
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Fusion Gene Summary

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Fusion Gene Sample Information

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Fusion ORF Analysis

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Fusion Amino Acid Sequences

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Fusion Protein Functional Features

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Fusion Protein Structure

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pLDDT scores

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Ramachandran Plot of Fusion Protein Structure

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Potential Active Site Information

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Potentially Interacting Small Molecules through Virtual Screening

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Biochemical Features of Small Molecules with ADME

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Drug Toxicity Information

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Fusion Protein-Protein Interaction

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Related drugs with this fusion protein

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Related disease with this fusion protein

Fusion Protein:HMGA2-WIF1

Fusion Protein Summary

check button Fusion gene summary
Fusion partner gene informationFusion gene name: HMGA2-WIF1
FusionPDB ID: 36860
FusionGDB2.0 ID: 36860
HgeneTgene
Gene symbol

HMGA2

WIF1

Gene ID

8091

11197

Gene namehigh mobility group AT-hook 2WNT inhibitory factor 1
SynonymsBABL|HMGI-C|HMGIC|LIPO|STQTL9WIF-1
Cytomap

12q14.3

12q14.3

Type of geneprotein-codingprotein-coding
Descriptionhigh mobility group protein HMGI-CHMGA2/KRT121P fusionhigh-mobility group (nonhistone chromosomal) protein isoform I-Cwnt inhibitory factor 1
Modification date2020032920200313
UniProtAcc

P52926

Q9Y5W5

Ensembl transtripts involved in fusion geneENST idsENST00000403681, ENST00000354636, 
ENST00000393577, ENST00000393578, 
ENST00000425208, ENST00000536545, 
ENST00000541363, 		ENST00000286574, 
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)* DoF score43 X 25 X 15=161257 X 11 X 6=462
# samples 4314
** MAII scorelog2(43/16125*10)=-5.22881869049588
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(14/462*10)=-1.72246602447109
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)

PubMed: HMGA2 [Title/Abstract] AND WIF1 [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)HMGA2(66345196)-WIF1(65445253), # samples:1
HMGA2(66232350)-WIF1(65471635), # samples:1
HMGA2(66357153)-WIF1(65471634), # samples:1
HMGA2(66232349)-WIF1(65462685), # samples:1
HMGA2(66232349)-WIF1(65448994), # samples:1
HMGA2(66232350)-WIF1(65445251), # samples:1
Anticipated loss of major functional domain due to fusion event.HMGA2-WIF1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
HMGA2-WIF1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
HMGA2-WIF1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
HMGA2-WIF1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
HMGA2-WIF1 seems lost the major protein functional domain in Hgene partner, which is a CGC due to the frame-shifted ORF.
HMGA2-WIF1 seems lost the major protein functional domain in Hgene partner, which is a transcription factor due to the frame-shifted ORF.
HMGA2-WIF1 seems lost the major protein functional domain in Tgene partner, which is a CGC due to the frame-shifted ORF.
HMGA2-WIF1 seems lost the major protein functional domain in Tgene partner, which is a essential gene due to the frame-shifted ORF.
HMGA2-WIF1 seems lost the major protein functional domain in Tgene partner, which is a tumor suppressor due to the frame-shifted ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgeneHMGA2

GO:0000122

negative regulation of transcription by RNA polymerase II

14627817

HgeneHMGA2

GO:0002062

chondrocyte differentiation

21484705

HgeneHMGA2

GO:0006284

base-excision repair

19465398

HgeneHMGA2

GO:0007095

mitotic G2 DNA damage checkpoint

16061642

HgeneHMGA2

GO:0010564

regulation of cell cycle process

14645522

HgeneHMGA2

GO:0010628

positive regulation of gene expression

18832382

HgeneHMGA2

GO:0031052

chromosome breakage

19549901

HgeneHMGA2

GO:0031507

heterochromatin assembly

16901784

HgeneHMGA2

GO:0035978

histone H2A-S139 phosphorylation

16061642

HgeneHMGA2

GO:0035986

senescence-associated heterochromatin focus assembly

16901784

HgeneHMGA2

GO:0035988

chondrocyte proliferation

21484705

HgeneHMGA2

GO:0042769

DNA damage response, detection of DNA damage

19465398

HgeneHMGA2

GO:0043065

positive regulation of apoptotic process

16061642

HgeneHMGA2

GO:0043066

negative regulation of apoptotic process

19465398

HgeneHMGA2

GO:0043392

negative regulation of DNA binding

14645522

HgeneHMGA2

GO:0043922

negative regulation by host of viral transcription

17005673

HgeneHMGA2

GO:0045869

negative regulation of single stranded viral RNA replication via double stranded DNA intermediate

17005673

HgeneHMGA2

GO:0045892

negative regulation of transcription, DNA-templated

18832382

HgeneHMGA2

GO:0045893

positive regulation of transcription, DNA-templated

15225648|15755872|17005673|17324944|17426251

HgeneHMGA2

GO:0045944

positive regulation of transcription by RNA polymerase II

14645522|18832382

HgeneHMGA2

GO:0071158

positive regulation of cell cycle arrest

16061642

HgeneHMGA2

GO:0071902

positive regulation of protein serine/threonine kinase activity

19549901

HgeneHMGA2

GO:0090402

oncogene-induced cell senescence

16901784

HgeneHMGA2

GO:2000648

positive regulation of stem cell proliferation

21484705

HgeneHMGA2

GO:2000679

positive regulation of transcription regulatory region DNA binding

18832382

HgeneHMGA2

GO:2000685

positive regulation of cellular response to X-ray

16061642

HgeneHMGA2

GO:2001022

positive regulation of response to DNA damage stimulus

16061642|19465398

HgeneHMGA2

GO:2001033

negative regulation of double-strand break repair via nonhomologous end joining

19549901

HgeneHMGA2

GO:2001038

regulation of cellular response to drug

16061642


check buttonFusion gene breakpoints across HMGA2 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check buttonFusion gene breakpoints across WIF1 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure


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Fusion Gene Sample Information

check buttonFusion gene information from FusionGDB2.0.
check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerKB3..HMGA2chr12

66232349

+WIF1chr12

65445164

-
ChimerKB3..HMGA2chr12

66232349

+WIF1chr12

65445250

-
ChimerKB3..HMGA2chr12

66232349

+WIF1chr12

65456356

-
ChimerKB3..HMGA2chr12

66232349

+WIF1chr12

65471634

-
ChimerKB3..HMGA2chr12

66232350

+WIF1chr12

65445164

-
ChimerKB3..HMGA2chr12

66357153

+WIF1chr12

65445164

-
ChiTaRS5.0N/AEF058195HMGA2chr12

66345196

+WIF1chr12

65445253

-
ChiTaRS5.0N/AEU263619HMGA2chr12

66232350

+WIF1chr12

65471635

-
ChiTaRS5.0N/AEU263620HMGA2chr12

66357153

+WIF1chr12

65471634

-
ChiTaRS5.0N/AEU263621HMGA2chr12

66232349

+WIF1chr12

65462685

-
ChiTaRS5.0N/AEU263622HMGA2chr12

66232349

+WIF1chr12

65448994

-
ChiTaRS5.0N/AEU263623HMGA2chr12

66232350

+WIF1chr12

65445251

-


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Fusion ORF Analysis


check buttonFusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000403681HMGA2chr1266232349+ENST00000286574WIF1chr1265445164-21481389114992292
ENST00000541363HMGA2chr1266232349+ENST00000286574WIF1chr1265445164-138162237365794
ENST00000393577HMGA2chr1266232349+ENST00000286574WIF1chr1265445164-115639714843294
ENST00000393578HMGA2chr1266232349+ENST00000286574WIF1chr1265445164-181910601663220
ENST00000425208HMGA2chr1266232349+ENST00000286574WIF1chr1265445164-181910601663220
ENST00000536545HMGA2chr1266232349+ENST00000286574WIF1chr1265445164-181910601663220
ENST00000354636HMGA2chr1266232349+ENST00000286574WIF1chr1265445164-181910601663220
ENST00000403681HMGA2chr1266232349+ENST00000286574WIF1chr1265445250-22341389114992292
ENST00000541363HMGA2chr1266232349+ENST00000286574WIF1chr1265445250-14676224902163
ENST00000393577HMGA2chr1266232349+ENST00000286574WIF1chr1265445250-124239714842692
ENST00000393578HMGA2chr1266232349+ENST00000286574WIF1chr1265445250-190510601663220
ENST00000425208HMGA2chr1266232349+ENST00000286574WIF1chr1265445250-190510601663220
ENST00000536545HMGA2chr1266232349+ENST00000286574WIF1chr1265445250-190510601663220
ENST00000354636HMGA2chr1266232349+ENST00000286574WIF1chr1265445250-190510601663220
ENST00000403681HMGA2chr1266232349+ENST00000286574WIF1chr1265471634-2964138911402240366
ENST00000541363HMGA2chr1266232349+ENST00000286574WIF1chr1265471634-21976223731473366
ENST00000393577HMGA2chr1266232349+ENST00000286574WIF1chr1265471634-19723971481248366
ENST00000393578HMGA2chr1266232349+ENST00000286574WIF1chr1265471634-263510608111911366
ENST00000425208HMGA2chr1266232349+ENST00000286574WIF1chr1265471634-263510608111911366
ENST00000536545HMGA2chr1266232349+ENST00000286574WIF1chr1265471634-263510608111911366
ENST00000354636HMGA2chr1266232349+ENST00000286574WIF1chr1265471634-263510608111911366
ENST00000403681HMGA2chr1266232350+ENST00000286574WIF1chr1265445164-21481389114992292
ENST00000541363HMGA2chr1266232350+ENST00000286574WIF1chr1265445164-138162237365794
ENST00000393577HMGA2chr1266232350+ENST00000286574WIF1chr1265445164-115639714843294
ENST00000393578HMGA2chr1266232350+ENST00000286574WIF1chr1265445164-181910601663220
ENST00000425208HMGA2chr1266232350+ENST00000286574WIF1chr1265445164-181910601663220
ENST00000536545HMGA2chr1266232350+ENST00000286574WIF1chr1265445164-181910601663220
ENST00000354636HMGA2chr1266232350+ENST00000286574WIF1chr1265445164-181910601663220
ENST00000403681HMGA2chr1266345196+ENST00000286574WIF1chr1265445253-22671422114992292
ENST00000393577HMGA2chr1266345196+ENST00000286574WIF1chr1265445253-1275430148459103
ENST00000403681HMGA2chr1266232350+ENST00000286574WIF1chr1265471635-2964138911402240366
ENST00000541363HMGA2chr1266232350+ENST00000286574WIF1chr1265471635-21976223731473366
ENST00000393577HMGA2chr1266232350+ENST00000286574WIF1chr1265471635-19723971481248366
ENST00000393578HMGA2chr1266232350+ENST00000286574WIF1chr1265471635-263510608111911366
ENST00000425208HMGA2chr1266232350+ENST00000286574WIF1chr1265471635-263510608111911366
ENST00000536545HMGA2chr1266232350+ENST00000286574WIF1chr1265471635-263510608111911366
ENST00000354636HMGA2chr1266232350+ENST00000286574WIF1chr1265471635-263510608111911366
ENST00000403681HMGA2chr1266232350+ENST00000286574WIF1chr1265445251-22341389114992292
ENST00000541363HMGA2chr1266232350+ENST00000286574WIF1chr1265445251-14676224902163
ENST00000393577HMGA2chr1266232350+ENST00000286574WIF1chr1265445251-124239714842692
ENST00000393578HMGA2chr1266232350+ENST00000286574WIF1chr1265445251-190510601663220
ENST00000425208HMGA2chr1266232350+ENST00000286574WIF1chr1265445251-190510601663220
ENST00000536545HMGA2chr1266232350+ENST00000286574WIF1chr1265445251-190510601663220
ENST00000354636HMGA2chr1266232350+ENST00000286574WIF1chr1265445251-190510601663220

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000403681ENST00000286574HMGA2chr1266345196+WIF1chr1265445253-0.269143020.730857
ENST00000393577ENST00000286574HMGA2chr1266345196+WIF1chr1265445253-0.217827420.7821726
ENST00000403681ENST00000286574HMGA2chr1266232350+WIF1chr1265471635-0.0027380380.997262
ENST00000541363ENST00000286574HMGA2chr1266232350+WIF1chr1265471635-0.0022237070.9977763
ENST00000393577ENST00000286574HMGA2chr1266232350+WIF1chr1265471635-0.0013984860.9986015
ENST00000393578ENST00000286574HMGA2chr1266232350+WIF1chr1265471635-0.0023745610.9976254
ENST00000425208ENST00000286574HMGA2chr1266232350+WIF1chr1265471635-0.0023745610.9976254
ENST00000536545ENST00000286574HMGA2chr1266232350+WIF1chr1265471635-0.0023745610.9976254
ENST00000354636ENST00000286574HMGA2chr1266232350+WIF1chr1265471635-0.0023745610.9976254
ENST00000403681ENST00000286574HMGA2chr1266232350+WIF1chr1265445251-0.54268070.4573193
ENST00000541363ENST00000286574HMGA2chr1266232350+WIF1chr1265445251-0.50831430.49168572
ENST00000393577ENST00000286574HMGA2chr1266232350+WIF1chr1265445251-0.481830840.51816916
ENST00000393578ENST00000286574HMGA2chr1266232350+WIF1chr1265445251-0.134694170.86530584
ENST00000425208ENST00000286574HMGA2chr1266232350+WIF1chr1265445251-0.134694170.86530584
ENST00000536545ENST00000286574HMGA2chr1266232350+WIF1chr1265445251-0.134694170.86530584
ENST00000354636ENST00000286574HMGA2chr1266232350+WIF1chr1265445251-0.134694170.86530584

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Fusion Amino Acid Sequences


check button For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>36860_36860_1_HMGA2-WIF1_HMGA2_chr12_66232349_ENST00000354636_WIF1_chr12_65445164_ENST00000286574_length(amino acids)=220AA_BP=
LNLGAGTQKTSSPGSARLVQDSGASSPSPSDSPVPPLPAPATLGGAVPPTTLSSACAPCPTLSRRSLPILLCFPTAQGTFNLNLFSLSLS
LSLSLSLSLSLSLAGWGEEEEEFFPRLTFQGTQFTPSLFPFQAASEVLPVPATPDPNPREEPLRPQSLSSFSLASLLLLLPPPPPPPPPP

--------------------------------------------------------------

>36860_36860_2_HMGA2-WIF1_HMGA2_chr12_66232349_ENST00000354636_WIF1_chr12_65445250_ENST00000286574_length(amino acids)=220AA_BP=
LNLGAGTQKTSSPGSARLVQDSGASSPSPSDSPVPPLPAPATLGGAVPPTTLSSACAPCPTLSRRSLPILLCFPTAQGTFNLNLFSLSLS
LSLSLSLSLSLSLAGWGEEEEEFFPRLTFQGTQFTPSLFPFQAASEVLPVPATPDPNPREEPLRPQSLSSFSLASLLLLLPPPPPPPPPP

--------------------------------------------------------------

>36860_36860_3_HMGA2-WIF1_HMGA2_chr12_66232349_ENST00000354636_WIF1_chr12_65471634_ENST00000286574_length(amino acids)=366AA_BP=83
MSARGEGAGQPSTSAQGQPAAPAPQKRGRGRPRKQQQEPTGEPSPKRPRGRPKGSKNKSPSKAAQKKAEATGEKRPRGRPRKWAEYFYEF
LSLRSLDKGIMADPTVNVPLLGTVPHKASVVQVGFPCLGKQDGVAAFEVDVIVMNSEGNTILQTPQNAIFFKTCQQAECPGGCRNGGFCN
ERRICECPDGFHGPHCEKALCTPRCMNGGLCVTPGFCICPPGFYGVNCDKANCSTTCFNGGTCFYPGKCICPPGLEGEQCEISKCPQPCR
NGGKCIGKSKCKCSKGYQGDLCSKPVCEPGCGAHGTCHEPNKCQCQEGWHGRHCNKRYEASLIHALRPAGAQLRQHTPSLKKAEERRDPP

--------------------------------------------------------------

>36860_36860_4_HMGA2-WIF1_HMGA2_chr12_66232349_ENST00000393577_WIF1_chr12_65445164_ENST00000286574_length(amino acids)=94AA_BP=
MSARGEGAGQPSTSAQGQPAAPAPQKRGRGRPRKQQQEPTGEPSPKRPRGRPKGSKNKSPSKAAQKKAEATGEKRPRGRPRKWRRDPPES

--------------------------------------------------------------

>36860_36860_5_HMGA2-WIF1_HMGA2_chr12_66232349_ENST00000393577_WIF1_chr12_65445250_ENST00000286574_length(amino acids)=92AA_BP=
MSARGEGAGQPSTSAQGQPAAPAPQKRGRGRPRKQQQEPTGEPSPKRPRGRPKGSKNKSPSKAAQKKAEATGEKRPRGRPRKWGTKPASY

--------------------------------------------------------------

>36860_36860_6_HMGA2-WIF1_HMGA2_chr12_66232349_ENST00000393577_WIF1_chr12_65471634_ENST00000286574_length(amino acids)=366AA_BP=83
MSARGEGAGQPSTSAQGQPAAPAPQKRGRGRPRKQQQEPTGEPSPKRPRGRPKGSKNKSPSKAAQKKAEATGEKRPRGRPRKWAEYFYEF
LSLRSLDKGIMADPTVNVPLLGTVPHKASVVQVGFPCLGKQDGVAAFEVDVIVMNSEGNTILQTPQNAIFFKTCQQAECPGGCRNGGFCN
ERRICECPDGFHGPHCEKALCTPRCMNGGLCVTPGFCICPPGFYGVNCDKANCSTTCFNGGTCFYPGKCICPPGLEGEQCEISKCPQPCR
NGGKCIGKSKCKCSKGYQGDLCSKPVCEPGCGAHGTCHEPNKCQCQEGWHGRHCNKRYEASLIHALRPAGAQLRQHTPSLKKAEERRDPP

--------------------------------------------------------------

>36860_36860_7_HMGA2-WIF1_HMGA2_chr12_66232349_ENST00000393578_WIF1_chr12_65445164_ENST00000286574_length(amino acids)=220AA_BP=
LNLGAGTQKTSSPGSARLVQDSGASSPSPSDSPVPPLPAPATLGGAVPPTTLSSACAPCPTLSRRSLPILLCFPTAQGTFNLNLFSLSLS
LSLSLSLSLSLSLAGWGEEEEEFFPRLTFQGTQFTPSLFPFQAASEVLPVPATPDPNPREEPLRPQSLSSFSLASLLLLLPPPPPPPPPP

--------------------------------------------------------------

>36860_36860_8_HMGA2-WIF1_HMGA2_chr12_66232349_ENST00000393578_WIF1_chr12_65445250_ENST00000286574_length(amino acids)=220AA_BP=
LNLGAGTQKTSSPGSARLVQDSGASSPSPSDSPVPPLPAPATLGGAVPPTTLSSACAPCPTLSRRSLPILLCFPTAQGTFNLNLFSLSLS
LSLSLSLSLSLSLAGWGEEEEEFFPRLTFQGTQFTPSLFPFQAASEVLPVPATPDPNPREEPLRPQSLSSFSLASLLLLLPPPPPPPPPP

--------------------------------------------------------------

>36860_36860_9_HMGA2-WIF1_HMGA2_chr12_66232349_ENST00000393578_WIF1_chr12_65471634_ENST00000286574_length(amino acids)=366AA_BP=83
MSARGEGAGQPSTSAQGQPAAPAPQKRGRGRPRKQQQEPTGEPSPKRPRGRPKGSKNKSPSKAAQKKAEATGEKRPRGRPRKWAEYFYEF
LSLRSLDKGIMADPTVNVPLLGTVPHKASVVQVGFPCLGKQDGVAAFEVDVIVMNSEGNTILQTPQNAIFFKTCQQAECPGGCRNGGFCN
ERRICECPDGFHGPHCEKALCTPRCMNGGLCVTPGFCICPPGFYGVNCDKANCSTTCFNGGTCFYPGKCICPPGLEGEQCEISKCPQPCR
NGGKCIGKSKCKCSKGYQGDLCSKPVCEPGCGAHGTCHEPNKCQCQEGWHGRHCNKRYEASLIHALRPAGAQLRQHTPSLKKAEERRDPP

--------------------------------------------------------------

>36860_36860_10_HMGA2-WIF1_HMGA2_chr12_66232349_ENST00000403681_WIF1_chr12_65445164_ENST00000286574_length(amino acids)=292AA_BP=
MPLASSQAPWLGGPPGLPPPHTFLPAQPPPLYPPTIRGGHSPHHHPLPKRKRVHAHTPHTLTLTHTHTHSSHLNLGAGTQKTSSPGSARL
VQDSGASSPSPSDSPVPPLPAPATLGGAVPPTTLSSACAPCPTLSRRSLPILLCFPTAQGTFNLNLFSLSLSLSLSLSLSLSLSLAGWGE
EEEEFFPRLTFQGTQFTPSLFPFQAASEVLPVPATPDPNPREEPLRPQSLSSFSLASLLLLLPPPPPPPPPPAPTHRRRRHRQRLLLSSS

--------------------------------------------------------------

>36860_36860_11_HMGA2-WIF1_HMGA2_chr12_66232349_ENST00000403681_WIF1_chr12_65445250_ENST00000286574_length(amino acids)=292AA_BP=
MPLASSQAPWLGGPPGLPPPHTFLPAQPPPLYPPTIRGGHSPHHHPLPKRKRVHAHTPHTLTLTHTHTHSSHLNLGAGTQKTSSPGSARL
VQDSGASSPSPSDSPVPPLPAPATLGGAVPPTTLSSACAPCPTLSRRSLPILLCFPTAQGTFNLNLFSLSLSLSLSLSLSLSLSLAGWGE
EEEEFFPRLTFQGTQFTPSLFPFQAASEVLPVPATPDPNPREEPLRPQSLSSFSLASLLLLLPPPPPPPPPPAPTHRRRRHRQRLLLSSS

--------------------------------------------------------------

>36860_36860_12_HMGA2-WIF1_HMGA2_chr12_66232349_ENST00000403681_WIF1_chr12_65471634_ENST00000286574_length(amino acids)=366AA_BP=83
MSARGEGAGQPSTSAQGQPAAPAPQKRGRGRPRKQQQEPTGEPSPKRPRGRPKGSKNKSPSKAAQKKAEATGEKRPRGRPRKWAEYFYEF
LSLRSLDKGIMADPTVNVPLLGTVPHKASVVQVGFPCLGKQDGVAAFEVDVIVMNSEGNTILQTPQNAIFFKTCQQAECPGGCRNGGFCN
ERRICECPDGFHGPHCEKALCTPRCMNGGLCVTPGFCICPPGFYGVNCDKANCSTTCFNGGTCFYPGKCICPPGLEGEQCEISKCPQPCR
NGGKCIGKSKCKCSKGYQGDLCSKPVCEPGCGAHGTCHEPNKCQCQEGWHGRHCNKRYEASLIHALRPAGAQLRQHTPSLKKAEERRDPP

--------------------------------------------------------------

>36860_36860_13_HMGA2-WIF1_HMGA2_chr12_66232349_ENST00000425208_WIF1_chr12_65445164_ENST00000286574_length(amino acids)=220AA_BP=
LNLGAGTQKTSSPGSARLVQDSGASSPSPSDSPVPPLPAPATLGGAVPPTTLSSACAPCPTLSRRSLPILLCFPTAQGTFNLNLFSLSLS
LSLSLSLSLSLSLAGWGEEEEEFFPRLTFQGTQFTPSLFPFQAASEVLPVPATPDPNPREEPLRPQSLSSFSLASLLLLLPPPPPPPPPP

--------------------------------------------------------------

>36860_36860_14_HMGA2-WIF1_HMGA2_chr12_66232349_ENST00000425208_WIF1_chr12_65445250_ENST00000286574_length(amino acids)=220AA_BP=
LNLGAGTQKTSSPGSARLVQDSGASSPSPSDSPVPPLPAPATLGGAVPPTTLSSACAPCPTLSRRSLPILLCFPTAQGTFNLNLFSLSLS
LSLSLSLSLSLSLAGWGEEEEEFFPRLTFQGTQFTPSLFPFQAASEVLPVPATPDPNPREEPLRPQSLSSFSLASLLLLLPPPPPPPPPP

--------------------------------------------------------------

>36860_36860_15_HMGA2-WIF1_HMGA2_chr12_66232349_ENST00000425208_WIF1_chr12_65471634_ENST00000286574_length(amino acids)=366AA_BP=83
MSARGEGAGQPSTSAQGQPAAPAPQKRGRGRPRKQQQEPTGEPSPKRPRGRPKGSKNKSPSKAAQKKAEATGEKRPRGRPRKWAEYFYEF
LSLRSLDKGIMADPTVNVPLLGTVPHKASVVQVGFPCLGKQDGVAAFEVDVIVMNSEGNTILQTPQNAIFFKTCQQAECPGGCRNGGFCN
ERRICECPDGFHGPHCEKALCTPRCMNGGLCVTPGFCICPPGFYGVNCDKANCSTTCFNGGTCFYPGKCICPPGLEGEQCEISKCPQPCR
NGGKCIGKSKCKCSKGYQGDLCSKPVCEPGCGAHGTCHEPNKCQCQEGWHGRHCNKRYEASLIHALRPAGAQLRQHTPSLKKAEERRDPP

--------------------------------------------------------------

>36860_36860_16_HMGA2-WIF1_HMGA2_chr12_66232349_ENST00000536545_WIF1_chr12_65445164_ENST00000286574_length(amino acids)=220AA_BP=
LNLGAGTQKTSSPGSARLVQDSGASSPSPSDSPVPPLPAPATLGGAVPPTTLSSACAPCPTLSRRSLPILLCFPTAQGTFNLNLFSLSLS
LSLSLSLSLSLSLAGWGEEEEEFFPRLTFQGTQFTPSLFPFQAASEVLPVPATPDPNPREEPLRPQSLSSFSLASLLLLLPPPPPPPPPP

--------------------------------------------------------------

>36860_36860_17_HMGA2-WIF1_HMGA2_chr12_66232349_ENST00000536545_WIF1_chr12_65445250_ENST00000286574_length(amino acids)=220AA_BP=
LNLGAGTQKTSSPGSARLVQDSGASSPSPSDSPVPPLPAPATLGGAVPPTTLSSACAPCPTLSRRSLPILLCFPTAQGTFNLNLFSLSLS
LSLSLSLSLSLSLAGWGEEEEEFFPRLTFQGTQFTPSLFPFQAASEVLPVPATPDPNPREEPLRPQSLSSFSLASLLLLLPPPPPPPPPP

--------------------------------------------------------------

>36860_36860_18_HMGA2-WIF1_HMGA2_chr12_66232349_ENST00000536545_WIF1_chr12_65471634_ENST00000286574_length(amino acids)=366AA_BP=83
MSARGEGAGQPSTSAQGQPAAPAPQKRGRGRPRKQQQEPTGEPSPKRPRGRPKGSKNKSPSKAAQKKAEATGEKRPRGRPRKWAEYFYEF
LSLRSLDKGIMADPTVNVPLLGTVPHKASVVQVGFPCLGKQDGVAAFEVDVIVMNSEGNTILQTPQNAIFFKTCQQAECPGGCRNGGFCN
ERRICECPDGFHGPHCEKALCTPRCMNGGLCVTPGFCICPPGFYGVNCDKANCSTTCFNGGTCFYPGKCICPPGLEGEQCEISKCPQPCR
NGGKCIGKSKCKCSKGYQGDLCSKPVCEPGCGAHGTCHEPNKCQCQEGWHGRHCNKRYEASLIHALRPAGAQLRQHTPSLKKAEERRDPP

--------------------------------------------------------------

>36860_36860_19_HMGA2-WIF1_HMGA2_chr12_66232349_ENST00000541363_WIF1_chr12_65445164_ENST00000286574_length(amino acids)=94AA_BP=
MSARGEGAGQPSTSAQGQPAAPAPQKRGRGRPRKQQQEPTGEPSPKRPRGRPKGSKNKSPSKAAQKKAEATGEKRPRGRPRKWRRDPPES

--------------------------------------------------------------

>36860_36860_20_HMGA2-WIF1_HMGA2_chr12_66232349_ENST00000541363_WIF1_chr12_65445250_ENST00000286574_length(amino acids)=163AA_BP=
MVLAAASWGGRVLSSEALGRQVVPGLKWTAAPRPHRVRSSCLPPPLPPLQPLRLDRPGAAQHLSGDEVIGLGTPAGRARAGGLRGLLLLR

--------------------------------------------------------------

>36860_36860_21_HMGA2-WIF1_HMGA2_chr12_66232349_ENST00000541363_WIF1_chr12_65471634_ENST00000286574_length(amino acids)=366AA_BP=83
MSARGEGAGQPSTSAQGQPAAPAPQKRGRGRPRKQQQEPTGEPSPKRPRGRPKGSKNKSPSKAAQKKAEATGEKRPRGRPRKWAEYFYEF
LSLRSLDKGIMADPTVNVPLLGTVPHKASVVQVGFPCLGKQDGVAAFEVDVIVMNSEGNTILQTPQNAIFFKTCQQAECPGGCRNGGFCN
ERRICECPDGFHGPHCEKALCTPRCMNGGLCVTPGFCICPPGFYGVNCDKANCSTTCFNGGTCFYPGKCICPPGLEGEQCEISKCPQPCR
NGGKCIGKSKCKCSKGYQGDLCSKPVCEPGCGAHGTCHEPNKCQCQEGWHGRHCNKRYEASLIHALRPAGAQLRQHTPSLKKAEERRDPP

--------------------------------------------------------------

>36860_36860_22_HMGA2-WIF1_HMGA2_chr12_66232350_ENST00000354636_WIF1_chr12_65445164_ENST00000286574_length(amino acids)=220AA_BP=
LNLGAGTQKTSSPGSARLVQDSGASSPSPSDSPVPPLPAPATLGGAVPPTTLSSACAPCPTLSRRSLPILLCFPTAQGTFNLNLFSLSLS
LSLSLSLSLSLSLAGWGEEEEEFFPRLTFQGTQFTPSLFPFQAASEVLPVPATPDPNPREEPLRPQSLSSFSLASLLLLLPPPPPPPPPP

--------------------------------------------------------------

>36860_36860_23_HMGA2-WIF1_HMGA2_chr12_66232350_ENST00000354636_WIF1_chr12_65445251_ENST00000286574_length(amino acids)=220AA_BP=
LNLGAGTQKTSSPGSARLVQDSGASSPSPSDSPVPPLPAPATLGGAVPPTTLSSACAPCPTLSRRSLPILLCFPTAQGTFNLNLFSLSLS
LSLSLSLSLSLSLAGWGEEEEEFFPRLTFQGTQFTPSLFPFQAASEVLPVPATPDPNPREEPLRPQSLSSFSLASLLLLLPPPPPPPPPP

--------------------------------------------------------------

>36860_36860_24_HMGA2-WIF1_HMGA2_chr12_66232350_ENST00000354636_WIF1_chr12_65471635_ENST00000286574_length(amino acids)=366AA_BP=83
MSARGEGAGQPSTSAQGQPAAPAPQKRGRGRPRKQQQEPTGEPSPKRPRGRPKGSKNKSPSKAAQKKAEATGEKRPRGRPRKWAEYFYEF
LSLRSLDKGIMADPTVNVPLLGTVPHKASVVQVGFPCLGKQDGVAAFEVDVIVMNSEGNTILQTPQNAIFFKTCQQAECPGGCRNGGFCN
ERRICECPDGFHGPHCEKALCTPRCMNGGLCVTPGFCICPPGFYGVNCDKANCSTTCFNGGTCFYPGKCICPPGLEGEQCEISKCPQPCR
NGGKCIGKSKCKCSKGYQGDLCSKPVCEPGCGAHGTCHEPNKCQCQEGWHGRHCNKRYEASLIHALRPAGAQLRQHTPSLKKAEERRDPP

--------------------------------------------------------------

>36860_36860_25_HMGA2-WIF1_HMGA2_chr12_66232350_ENST00000393577_WIF1_chr12_65445164_ENST00000286574_length(amino acids)=94AA_BP=
MSARGEGAGQPSTSAQGQPAAPAPQKRGRGRPRKQQQEPTGEPSPKRPRGRPKGSKNKSPSKAAQKKAEATGEKRPRGRPRKWRRDPPES

--------------------------------------------------------------

>36860_36860_26_HMGA2-WIF1_HMGA2_chr12_66232350_ENST00000393577_WIF1_chr12_65445251_ENST00000286574_length(amino acids)=92AA_BP=
MSARGEGAGQPSTSAQGQPAAPAPQKRGRGRPRKQQQEPTGEPSPKRPRGRPKGSKNKSPSKAAQKKAEATGEKRPRGRPRKWGTKPASY

--------------------------------------------------------------

>36860_36860_27_HMGA2-WIF1_HMGA2_chr12_66232350_ENST00000393577_WIF1_chr12_65471635_ENST00000286574_length(amino acids)=366AA_BP=83
MSARGEGAGQPSTSAQGQPAAPAPQKRGRGRPRKQQQEPTGEPSPKRPRGRPKGSKNKSPSKAAQKKAEATGEKRPRGRPRKWAEYFYEF
LSLRSLDKGIMADPTVNVPLLGTVPHKASVVQVGFPCLGKQDGVAAFEVDVIVMNSEGNTILQTPQNAIFFKTCQQAECPGGCRNGGFCN
ERRICECPDGFHGPHCEKALCTPRCMNGGLCVTPGFCICPPGFYGVNCDKANCSTTCFNGGTCFYPGKCICPPGLEGEQCEISKCPQPCR
NGGKCIGKSKCKCSKGYQGDLCSKPVCEPGCGAHGTCHEPNKCQCQEGWHGRHCNKRYEASLIHALRPAGAQLRQHTPSLKKAEERRDPP

--------------------------------------------------------------

>36860_36860_28_HMGA2-WIF1_HMGA2_chr12_66232350_ENST00000393578_WIF1_chr12_65445164_ENST00000286574_length(amino acids)=220AA_BP=
LNLGAGTQKTSSPGSARLVQDSGASSPSPSDSPVPPLPAPATLGGAVPPTTLSSACAPCPTLSRRSLPILLCFPTAQGTFNLNLFSLSLS
LSLSLSLSLSLSLAGWGEEEEEFFPRLTFQGTQFTPSLFPFQAASEVLPVPATPDPNPREEPLRPQSLSSFSLASLLLLLPPPPPPPPPP

--------------------------------------------------------------

>36860_36860_29_HMGA2-WIF1_HMGA2_chr12_66232350_ENST00000393578_WIF1_chr12_65445251_ENST00000286574_length(amino acids)=220AA_BP=
LNLGAGTQKTSSPGSARLVQDSGASSPSPSDSPVPPLPAPATLGGAVPPTTLSSACAPCPTLSRRSLPILLCFPTAQGTFNLNLFSLSLS
LSLSLSLSLSLSLAGWGEEEEEFFPRLTFQGTQFTPSLFPFQAASEVLPVPATPDPNPREEPLRPQSLSSFSLASLLLLLPPPPPPPPPP

--------------------------------------------------------------

>36860_36860_30_HMGA2-WIF1_HMGA2_chr12_66232350_ENST00000393578_WIF1_chr12_65471635_ENST00000286574_length(amino acids)=366AA_BP=83
MSARGEGAGQPSTSAQGQPAAPAPQKRGRGRPRKQQQEPTGEPSPKRPRGRPKGSKNKSPSKAAQKKAEATGEKRPRGRPRKWAEYFYEF
LSLRSLDKGIMADPTVNVPLLGTVPHKASVVQVGFPCLGKQDGVAAFEVDVIVMNSEGNTILQTPQNAIFFKTCQQAECPGGCRNGGFCN
ERRICECPDGFHGPHCEKALCTPRCMNGGLCVTPGFCICPPGFYGVNCDKANCSTTCFNGGTCFYPGKCICPPGLEGEQCEISKCPQPCR
NGGKCIGKSKCKCSKGYQGDLCSKPVCEPGCGAHGTCHEPNKCQCQEGWHGRHCNKRYEASLIHALRPAGAQLRQHTPSLKKAEERRDPP

--------------------------------------------------------------

>36860_36860_31_HMGA2-WIF1_HMGA2_chr12_66232350_ENST00000403681_WIF1_chr12_65445164_ENST00000286574_length(amino acids)=292AA_BP=
MPLASSQAPWLGGPPGLPPPHTFLPAQPPPLYPPTIRGGHSPHHHPLPKRKRVHAHTPHTLTLTHTHTHSSHLNLGAGTQKTSSPGSARL
VQDSGASSPSPSDSPVPPLPAPATLGGAVPPTTLSSACAPCPTLSRRSLPILLCFPTAQGTFNLNLFSLSLSLSLSLSLSLSLSLAGWGE
EEEEFFPRLTFQGTQFTPSLFPFQAASEVLPVPATPDPNPREEPLRPQSLSSFSLASLLLLLPPPPPPPPPPAPTHRRRRHRQRLLLSSS

--------------------------------------------------------------

>36860_36860_32_HMGA2-WIF1_HMGA2_chr12_66232350_ENST00000403681_WIF1_chr12_65445251_ENST00000286574_length(amino acids)=292AA_BP=
MPLASSQAPWLGGPPGLPPPHTFLPAQPPPLYPPTIRGGHSPHHHPLPKRKRVHAHTPHTLTLTHTHTHSSHLNLGAGTQKTSSPGSARL
VQDSGASSPSPSDSPVPPLPAPATLGGAVPPTTLSSACAPCPTLSRRSLPILLCFPTAQGTFNLNLFSLSLSLSLSLSLSLSLSLAGWGE
EEEEFFPRLTFQGTQFTPSLFPFQAASEVLPVPATPDPNPREEPLRPQSLSSFSLASLLLLLPPPPPPPPPPAPTHRRRRHRQRLLLSSS

--------------------------------------------------------------

>36860_36860_33_HMGA2-WIF1_HMGA2_chr12_66232350_ENST00000403681_WIF1_chr12_65471635_ENST00000286574_length(amino acids)=366AA_BP=83
MSARGEGAGQPSTSAQGQPAAPAPQKRGRGRPRKQQQEPTGEPSPKRPRGRPKGSKNKSPSKAAQKKAEATGEKRPRGRPRKWAEYFYEF
LSLRSLDKGIMADPTVNVPLLGTVPHKASVVQVGFPCLGKQDGVAAFEVDVIVMNSEGNTILQTPQNAIFFKTCQQAECPGGCRNGGFCN
ERRICECPDGFHGPHCEKALCTPRCMNGGLCVTPGFCICPPGFYGVNCDKANCSTTCFNGGTCFYPGKCICPPGLEGEQCEISKCPQPCR
NGGKCIGKSKCKCSKGYQGDLCSKPVCEPGCGAHGTCHEPNKCQCQEGWHGRHCNKRYEASLIHALRPAGAQLRQHTPSLKKAEERRDPP

--------------------------------------------------------------

>36860_36860_34_HMGA2-WIF1_HMGA2_chr12_66232350_ENST00000425208_WIF1_chr12_65445164_ENST00000286574_length(amino acids)=220AA_BP=
LNLGAGTQKTSSPGSARLVQDSGASSPSPSDSPVPPLPAPATLGGAVPPTTLSSACAPCPTLSRRSLPILLCFPTAQGTFNLNLFSLSLS
LSLSLSLSLSLSLAGWGEEEEEFFPRLTFQGTQFTPSLFPFQAASEVLPVPATPDPNPREEPLRPQSLSSFSLASLLLLLPPPPPPPPPP

--------------------------------------------------------------

>36860_36860_35_HMGA2-WIF1_HMGA2_chr12_66232350_ENST00000425208_WIF1_chr12_65445251_ENST00000286574_length(amino acids)=220AA_BP=
LNLGAGTQKTSSPGSARLVQDSGASSPSPSDSPVPPLPAPATLGGAVPPTTLSSACAPCPTLSRRSLPILLCFPTAQGTFNLNLFSLSLS
LSLSLSLSLSLSLAGWGEEEEEFFPRLTFQGTQFTPSLFPFQAASEVLPVPATPDPNPREEPLRPQSLSSFSLASLLLLLPPPPPPPPPP

--------------------------------------------------------------

>36860_36860_36_HMGA2-WIF1_HMGA2_chr12_66232350_ENST00000425208_WIF1_chr12_65471635_ENST00000286574_length(amino acids)=366AA_BP=83
MSARGEGAGQPSTSAQGQPAAPAPQKRGRGRPRKQQQEPTGEPSPKRPRGRPKGSKNKSPSKAAQKKAEATGEKRPRGRPRKWAEYFYEF
LSLRSLDKGIMADPTVNVPLLGTVPHKASVVQVGFPCLGKQDGVAAFEVDVIVMNSEGNTILQTPQNAIFFKTCQQAECPGGCRNGGFCN
ERRICECPDGFHGPHCEKALCTPRCMNGGLCVTPGFCICPPGFYGVNCDKANCSTTCFNGGTCFYPGKCICPPGLEGEQCEISKCPQPCR
NGGKCIGKSKCKCSKGYQGDLCSKPVCEPGCGAHGTCHEPNKCQCQEGWHGRHCNKRYEASLIHALRPAGAQLRQHTPSLKKAEERRDPP

--------------------------------------------------------------

>36860_36860_37_HMGA2-WIF1_HMGA2_chr12_66232350_ENST00000536545_WIF1_chr12_65445164_ENST00000286574_length(amino acids)=220AA_BP=
LNLGAGTQKTSSPGSARLVQDSGASSPSPSDSPVPPLPAPATLGGAVPPTTLSSACAPCPTLSRRSLPILLCFPTAQGTFNLNLFSLSLS
LSLSLSLSLSLSLAGWGEEEEEFFPRLTFQGTQFTPSLFPFQAASEVLPVPATPDPNPREEPLRPQSLSSFSLASLLLLLPPPPPPPPPP

--------------------------------------------------------------

>36860_36860_38_HMGA2-WIF1_HMGA2_chr12_66232350_ENST00000536545_WIF1_chr12_65445251_ENST00000286574_length(amino acids)=220AA_BP=
LNLGAGTQKTSSPGSARLVQDSGASSPSPSDSPVPPLPAPATLGGAVPPTTLSSACAPCPTLSRRSLPILLCFPTAQGTFNLNLFSLSLS
LSLSLSLSLSLSLAGWGEEEEEFFPRLTFQGTQFTPSLFPFQAASEVLPVPATPDPNPREEPLRPQSLSSFSLASLLLLLPPPPPPPPPP

--------------------------------------------------------------

>36860_36860_39_HMGA2-WIF1_HMGA2_chr12_66232350_ENST00000536545_WIF1_chr12_65471635_ENST00000286574_length(amino acids)=366AA_BP=83
MSARGEGAGQPSTSAQGQPAAPAPQKRGRGRPRKQQQEPTGEPSPKRPRGRPKGSKNKSPSKAAQKKAEATGEKRPRGRPRKWAEYFYEF
LSLRSLDKGIMADPTVNVPLLGTVPHKASVVQVGFPCLGKQDGVAAFEVDVIVMNSEGNTILQTPQNAIFFKTCQQAECPGGCRNGGFCN
ERRICECPDGFHGPHCEKALCTPRCMNGGLCVTPGFCICPPGFYGVNCDKANCSTTCFNGGTCFYPGKCICPPGLEGEQCEISKCPQPCR
NGGKCIGKSKCKCSKGYQGDLCSKPVCEPGCGAHGTCHEPNKCQCQEGWHGRHCNKRYEASLIHALRPAGAQLRQHTPSLKKAEERRDPP

--------------------------------------------------------------

>36860_36860_40_HMGA2-WIF1_HMGA2_chr12_66232350_ENST00000541363_WIF1_chr12_65445164_ENST00000286574_length(amino acids)=94AA_BP=
MSARGEGAGQPSTSAQGQPAAPAPQKRGRGRPRKQQQEPTGEPSPKRPRGRPKGSKNKSPSKAAQKKAEATGEKRPRGRPRKWRRDPPES

--------------------------------------------------------------

>36860_36860_41_HMGA2-WIF1_HMGA2_chr12_66232350_ENST00000541363_WIF1_chr12_65445251_ENST00000286574_length(amino acids)=163AA_BP=
MVLAAASWGGRVLSSEALGRQVVPGLKWTAAPRPHRVRSSCLPPPLPPLQPLRLDRPGAAQHLSGDEVIGLGTPAGRARAGGLRGLLLLR

--------------------------------------------------------------

>36860_36860_42_HMGA2-WIF1_HMGA2_chr12_66232350_ENST00000541363_WIF1_chr12_65471635_ENST00000286574_length(amino acids)=366AA_BP=83
MSARGEGAGQPSTSAQGQPAAPAPQKRGRGRPRKQQQEPTGEPSPKRPRGRPKGSKNKSPSKAAQKKAEATGEKRPRGRPRKWAEYFYEF
LSLRSLDKGIMADPTVNVPLLGTVPHKASVVQVGFPCLGKQDGVAAFEVDVIVMNSEGNTILQTPQNAIFFKTCQQAECPGGCRNGGFCN
ERRICECPDGFHGPHCEKALCTPRCMNGGLCVTPGFCICPPGFYGVNCDKANCSTTCFNGGTCFYPGKCICPPGLEGEQCEISKCPQPCR
NGGKCIGKSKCKCSKGYQGDLCSKPVCEPGCGAHGTCHEPNKCQCQEGWHGRHCNKRYEASLIHALRPAGAQLRQHTPSLKKAEERRDPP

--------------------------------------------------------------

>36860_36860_43_HMGA2-WIF1_HMGA2_chr12_66345196_ENST00000393577_WIF1_chr12_65445253_ENST00000286574_length(amino acids)=103AA_BP=
MSARGEGAGQPSTSAQGQPAAPAPQKRGRGRPRKQQQEPTGEPSPKRPRGRPKGSKNKSPSKAAQKKAEATGEKRPRGRPRKWPQQVVQK

--------------------------------------------------------------

>36860_36860_44_HMGA2-WIF1_HMGA2_chr12_66345196_ENST00000403681_WIF1_chr12_65445253_ENST00000286574_length(amino acids)=292AA_BP=
MPLASSQAPWLGGPPGLPPPHTFLPAQPPPLYPPTIRGGHSPHHHPLPKRKRVHAHTPHTLTLTHTHTHSSHLNLGAGTQKTSSPGSARL
VQDSGASSPSPSDSPVPPLPAPATLGGAVPPTTLSSACAPCPTLSRRSLPILLCFPTAQGTFNLNLFSLSLSLSLSLSLSLSLSLAGWGE
EEEEFFPRLTFQGTQFTPSLFPFQAASEVLPVPATPDPNPREEPLRPQSLSSFSLASLLLLLPPPPPPPPPPAPTHRRRRHRQRLLLSSS

--------------------------------------------------------------

Top

Fusion Protein Functional Features


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr12:66345196/chr12:65445253)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
HMGA2

P52926

WIF1

Q9Y5W5

FUNCTION: Functions as a transcriptional regulator. Functions in cell cycle regulation through CCNA2. Plays an important role in chromosome condensation during the meiotic G2/M transition of spermatocytes. Plays a role in postnatal myogenesis, is involved in satellite cell activation (By similarity). Positively regulates IGF2 expression through PLAG1 and in a PLAG1-independent manner (PubMed:28796236). {ECO:0000250|UniProtKB:P52927, ECO:0000269|PubMed:14645522, ECO:0000269|PubMed:28796236}.FUNCTION: Binds to WNT proteins and inhibits their activities. May be involved in mesoderm segmentation.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page

* Minus value of BPloci means that the break pointn is located before the CDS.
- Retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneHMGA2chr12:66232350chr12:65445251ENST00000354636+3424_3483.0107.0DNA bindingNote=A.T hook 1
HgeneHMGA2chr12:66232350chr12:65445251ENST00000354636+3444_5483.0107.0DNA bindingNote=A.T hook 2
HgeneHMGA2chr12:66232350chr12:65445251ENST00000354636+3471_8283.0107.0DNA bindingNote=A.T hook 3
HgeneHMGA2chr12:66232350chr12:65445251ENST00000393578+3424_3483.091.0DNA bindingNote=A.T hook 1
HgeneHMGA2chr12:66232350chr12:65445251ENST00000393578+3444_5483.091.0DNA bindingNote=A.T hook 2
HgeneHMGA2chr12:66232350chr12:65445251ENST00000393578+3471_8283.091.0DNA bindingNote=A.T hook 3
HgeneHMGA2chr12:66232350chr12:65445251ENST00000403681+3524_3483.0110.0DNA bindingNote=A.T hook 1
HgeneHMGA2chr12:66232350chr12:65445251ENST00000403681+3544_5483.0110.0DNA bindingNote=A.T hook 2
HgeneHMGA2chr12:66232350chr12:65445251ENST00000403681+3571_8283.0110.0DNA bindingNote=A.T hook 3
HgeneHMGA2chr12:66232350chr12:65445251ENST00000425208+3424_3483.093.0DNA bindingNote=A.T hook 1
HgeneHMGA2chr12:66232350chr12:65445251ENST00000425208+3444_5483.093.0DNA bindingNote=A.T hook 2
HgeneHMGA2chr12:66232350chr12:65445251ENST00000425208+3471_8283.093.0DNA bindingNote=A.T hook 3
HgeneHMGA2chr12:66232350chr12:65471635ENST00000354636+3424_3483.0107.0DNA bindingNote=A.T hook 1
HgeneHMGA2chr12:66232350chr12:65471635ENST00000354636+3444_5483.0107.0DNA bindingNote=A.T hook 2
HgeneHMGA2chr12:66232350chr12:65471635ENST00000354636+3471_8283.0107.0DNA bindingNote=A.T hook 3
HgeneHMGA2chr12:66232350chr12:65471635ENST00000393578+3424_3483.091.0DNA bindingNote=A.T hook 1
HgeneHMGA2chr12:66232350chr12:65471635ENST00000393578+3444_5483.091.0DNA bindingNote=A.T hook 2
HgeneHMGA2chr12:66232350chr12:65471635ENST00000393578+3471_8283.091.0DNA bindingNote=A.T hook 3
HgeneHMGA2chr12:66232350chr12:65471635ENST00000403681+3524_3483.0110.0DNA bindingNote=A.T hook 1
HgeneHMGA2chr12:66232350chr12:65471635ENST00000403681+3544_5483.0110.0DNA bindingNote=A.T hook 2
HgeneHMGA2chr12:66232350chr12:65471635ENST00000403681+3571_8283.0110.0DNA bindingNote=A.T hook 3
HgeneHMGA2chr12:66232350chr12:65471635ENST00000425208+3424_3483.093.0DNA bindingNote=A.T hook 1
HgeneHMGA2chr12:66232350chr12:65471635ENST00000425208+3444_5483.093.0DNA bindingNote=A.T hook 2
HgeneHMGA2chr12:66232350chr12:65471635ENST00000425208+3471_8283.093.0DNA bindingNote=A.T hook 3
HgeneHMGA2chr12:66345196chr12:65445253ENST00000403681+4524_3494.0110.0DNA bindingNote=A.T hook 1
HgeneHMGA2chr12:66345196chr12:65445253ENST00000403681+4544_5494.0110.0DNA bindingNote=A.T hook 2
HgeneHMGA2chr12:66345196chr12:65445253ENST00000403681+4571_8294.0110.0DNA bindingNote=A.T hook 3
TgeneWIF1chr12:66232350chr12:65471635ENST00000286574110178_21096.0380.0DomainEGF-like 1
TgeneWIF1chr12:66232350chr12:65471635ENST00000286574110211_24296.0380.0DomainEGF-like 2
TgeneWIF1chr12:66232350chr12:65471635ENST00000286574110243_27196.0380.0DomainEGF-like 3
TgeneWIF1chr12:66232350chr12:65471635ENST00000286574110274_30696.0380.0DomainEGF-like 4
TgeneWIF1chr12:66232350chr12:65471635ENST00000286574110307_33896.0380.0DomainEGF-like 5

- Not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneHMGA2chr12:66345196chr12:65445253ENST00000354636+1424_340107.0DNA bindingNote=A.T hook 1
HgeneHMGA2chr12:66345196chr12:65445253ENST00000354636+1444_540107.0DNA bindingNote=A.T hook 2
HgeneHMGA2chr12:66345196chr12:65445253ENST00000354636+1471_820107.0DNA bindingNote=A.T hook 3
HgeneHMGA2chr12:66345196chr12:65445253ENST00000393578+1424_34091.0DNA bindingNote=A.T hook 1
HgeneHMGA2chr12:66345196chr12:65445253ENST00000393578+1444_54091.0DNA bindingNote=A.T hook 2
HgeneHMGA2chr12:66345196chr12:65445253ENST00000393578+1471_82091.0DNA bindingNote=A.T hook 3
HgeneHMGA2chr12:66345196chr12:65445253ENST00000425208+1424_34093.0DNA bindingNote=A.T hook 1
HgeneHMGA2chr12:66345196chr12:65445253ENST00000425208+1444_54093.0DNA bindingNote=A.T hook 2
HgeneHMGA2chr12:66345196chr12:65445253ENST00000425208+1471_82093.0DNA bindingNote=A.T hook 3
TgeneWIF1chr12:66232350chr12:65445251ENST00000286574810178_210339.3333333333333380.0DomainEGF-like 1
TgeneWIF1chr12:66232350chr12:65445251ENST00000286574810211_242339.3333333333333380.0DomainEGF-like 2
TgeneWIF1chr12:66232350chr12:65445251ENST00000286574810243_271339.3333333333333380.0DomainEGF-like 3
TgeneWIF1chr12:66232350chr12:65445251ENST00000286574810274_306339.3333333333333380.0DomainEGF-like 4
TgeneWIF1chr12:66232350chr12:65445251ENST00000286574810307_338339.3333333333333380.0DomainEGF-like 5
TgeneWIF1chr12:66232350chr12:65445251ENST0000028657481038_177339.3333333333333380.0DomainWIF
TgeneWIF1chr12:66232350chr12:65471635ENST0000028657411038_17796.0380.0DomainWIF
TgeneWIF1chr12:66345196chr12:65445253ENST00000286574810178_210339.3333333333333380.0DomainEGF-like 1
TgeneWIF1chr12:66345196chr12:65445253ENST00000286574810211_242339.3333333333333380.0DomainEGF-like 2
TgeneWIF1chr12:66345196chr12:65445253ENST00000286574810243_271339.3333333333333380.0DomainEGF-like 3
TgeneWIF1chr12:66345196chr12:65445253ENST00000286574810274_306339.3333333333333380.0DomainEGF-like 4
TgeneWIF1chr12:66345196chr12:65445253ENST00000286574810307_338339.3333333333333380.0DomainEGF-like 5
TgeneWIF1chr12:66345196chr12:65445253ENST0000028657481038_177339.3333333333333380.0DomainWIF


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Fusion Protein Structures

check button PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.
Fusion protein PDB link (fusion AA seq ID in FusionPDB)HgeneHchrHbpHstrandTgeneTchrTbpTstrandAA seqLen(AA seq)
PDB file (4) >>>4.pdbFusion protein BP residue:
CIF file (4) >>>4.cif		HMGA2chr1266232349+WIF1chr1265445250-
MSARGEGAGQPSTSAQGQPAAPAPQKRGRGRPRKQQQEPTGEPSPKRPRG
92
3D view using mol* of 4 (AA BP:)
PDB file (5) >>>5.pdbFusion protein BP residue:
CIF file (5) >>>5.cif		HMGA2chr1266232349+WIF1chr1265445164-
MSARGEGAGQPSTSAQGQPAAPAPQKRGRGRPRKQQQEPTGEPSPKRPRG
94
3D view using mol* of 5 (AA BP:)
PDB file (12) >>>12.pdbFusion protein BP residue:
CIF file (12) >>>12.cif		HMGA2chr1266232349+WIF1chr1265445250-
MVLAAASWGGRVLSSEALGRQVVPGLKWTAAPRPHRVRSSCLPPPLPPLQ
PLRLDRPGAAQHLSGDEVIGLGTPAGRARAGGLRGLLLLRLPPLPPSTPD
VQRLPKREEGRRRRRGGGAAGGGGGGGWVPEVEVAVEVEVARGGGKRGRR
163
3D view using mol* of 12 (AA BP:)
PDB file (31) >>>31.pdbFusion protein BP residue:
CIF file (31) >>>31.cif		HMGA2chr1266232349+WIF1chr1265445164-
LNLGAGTQKTSSPGSARLVQDSGASSPSPSDSPVPPLPAPATLGGAVPPT
TLSSACAPCPTLSRRSLPILLCFPTAQGTFNLNLFSLSLSLSLSLSLSLS
LSLAGWGEEEEEFFPRLTFQGTQFTPSLFPFQAASEVLPVPATPDPNPRE
EPLRPQSLSSFSLASLLLLLPPPPPPPPPPAPTHRRRRHRQRLLLSSSSS
220
3D view using mol* of 31 (AA BP:)
PDB file (60) >>>60.pdbFusion protein BP residue:
CIF file (60) >>>60.cif		HMGA2chr1266232349+WIF1chr1265445164-
MPLASSQAPWLGGPPGLPPPHTFLPAQPPPLYPPTIRGGHSPHHHPLPKR
KRVHAHTPHTLTLTHTHTHSSHLNLGAGTQKTSSPGSARLVQDSGASSPS
PSDSPVPPLPAPATLGGAVPPTTLSSACAPCPTLSRRSLPILLCFPTAQG
TFNLNLFSLSLSLSLSLSLSLSLSLAGWGEEEEEFFPRLTFQGTQFTPSL
FPFQAASEVLPVPATPDPNPREEPLRPQSLSSFSLASLLLLLPPPPPPPP
292
3D view using mol* of 60 (AA BP:)
PDB file (118) >>>118.pdbFusion protein BP residue: 83
CIF file (118) >>>118.cif		HMGA2chr1266232349+WIF1chr1265471634-
MSARGEGAGQPSTSAQGQPAAPAPQKRGRGRPRKQQQEPTGEPSPKRPRG
RPKGSKNKSPSKAAQKKAEATGEKRPRGRPRKWAEYFYEFLSLRSLDKGI
MADPTVNVPLLGTVPHKASVVQVGFPCLGKQDGVAAFEVDVIVMNSEGNT
ILQTPQNAIFFKTCQQAECPGGCRNGGFCNERRICECPDGFHGPHCEKAL
CTPRCMNGGLCVTPGFCICPPGFYGVNCDKANCSTTCFNGGTCFYPGKCI
CPPGLEGEQCEISKCPQPCRNGGKCIGKSKCKCSKGYQGDLCSKPVCEPG
CGAHGTCHEPNKCQCQEGWHGRHCNKRYEASLIHALRPAGAQLRQHTPSL
366
3D view using mol* of 118 (AA BP:83)


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pLDDT score distribution

check button pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.
HMGA2_pLDDT.png
all structure
all structure
WIF1_pLDDT.png
all structure
all structure

check button pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.
HMGA2_WIF1_4_pLDDT.png (AA BP:)
all structure
HMGA2_WIF1_4_pLDDT_and_active_sites.png (AA BP:)
all structure
HMGA2_WIF1_4_violinplot.png (AA BP:)
all structure
HMGA2_WIF1_5_pLDDT.png (AA BP:)
all structure
HMGA2_WIF1_5_pLDDT_and_active_sites.png (AA BP:)
all structure
HMGA2_WIF1_5_violinplot.png (AA BP:)
all structure
HMGA2_WIF1_12_pLDDT.png (AA BP:)
all structure
HMGA2_WIF1_12_pLDDT_and_active_sites.png (AA BP:)
all structure
HMGA2_WIF1_12_violinplot.png (AA BP:)
all structure
HMGA2_WIF1_31_PAE.png (AA BP:)
all structure
HMGA2_WIF1_31_pLDDT.png (AA BP:)
all structure
HMGA2_WIF1_31_pLDDT_and_active_sites.png (AA BP:)
all structure
HMGA2_WIF1_31_violinplot.png (AA BP:)
all structure
HMGA2_WIF1_60_PAE.png (AA BP:)
all structure
HMGA2_WIF1_60_pLDDT.png (AA BP:)
all structure
HMGA2_WIF1_118_PAE.png (AA BP:83)
all structure
HMGA2_WIF1_118_pLDDT.png (AA BP:83)
all structure
HMGA2_WIF1_118_pLDDT_and_active_sites.png (AA BP:83)
all structure
HMGA2_WIF1_118_violinplot.png (AA BP:83)
all structure


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Ramachandran Plot of Fusion Protein Structure


check button Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.
Fusion AA seq ID in FusionPDB and their Ramachandran plots
HMGA2_WIF1_4.png
all structure
HMGA2_WIF1_5.png
all structure
HMGA2_WIF1_12.png
all structure
HMGA2_WIF1_31.png
all structure
HMGA2_WIF1_118.png
all structure

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Potential Active Site Information


check button The potential binding sites of these fusion proteins were identified using SiteMap, a module of the Schrodinger suite.
Fusion AA seq ID in FusionPDBSite scoreSizeD scoreVolumeExposureEnclosureContactPhobicPhilicBalanceDon/AccResidues
40.407120.26818.8650.7650.5280.9290.1411.150.1231.391Chain A: 73,74,75,76,77,78,79,81
50.34670.24122.9810.8920.4960.6790.0780.9380.0830.879Chain A: 75,76,77,78,79,80,81,82
121.041311.116265.4820.550.6450.8761.3820.6472.1370.956Chain A: 3,4,5,6,7,8,13,15,18,19,20,21,22,23,24,26
,27,28,29,30,31
310.6240.57988.8370.7710.5440.6690.4510.6380.7072.29Chain A: 71,72,73,75,80,81,84,85
1180.869770.875175.9590.6580.6220.870.4210.9920.4240.893Chain A: 105,106,107,122,123,124,125,126,162,175,1
76,177,178,186,187,188,189,191,213,214

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Potentially Interacting Small Molecules through Virtual Screening


check button The FDA-approved small molecule library molecules were subjected to virtual screening using the Glide.
Fusion AA seq ID in FusionPDBZINC IDDrugBank IDDrug nameDocking scoreGlide gscore

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check button Drug information from DrugBank of the top 20 interacting small molecules.
ZINC IDDrugBank IDDrug nameDrug typeSMILESDrug group

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Biochemical Features of Small Molecules


check button ADME (Absorption, Distribution, Metabolism, and Excretion) of drugs using QikProp(v3.9)
ZINC IDmol_MWdipoleSASAFOSAFISAPISAWPSAvolumedonorHBaccptHBIPHuman Oral AbsorptionPercent Human Oral AbsorptionRule Of FiveRule Of Three


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Drug Toxicity Information


check button Toxicity information of individual drugs using eToxPred
ZINC IDSmileSurface AccessibilityToxicity


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Fusion Protein-Protein Interaction


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)
GenePPI interactors
HMGA2RELA, NFKB1, PIAS3, RB1, PRMT6, NPM1, SMAD1, SMAD5, SMAD9, PRKCA, HDGF, PTBP1, APEX1, SYNCRIP, XRCC6, PSIP1, PA2G4, PCBP2, E4F1, OBSL1, HIST1H3A, CREB1, NFATC1, PCGF1, POU5F1, DLST, EZH2, SUZ12, MYC, TP53, MDM2, CSK, VRK1, HIST1H1B, PRKAG2, DUX4, DUX4L9, SYDE1, ARHGAP21, ARHGAP39, Plekhg5, ZNF263, MAFB, KAT2B, HIST1H2BG, LMNB1, NDUFAF7, CSNK2A1, LIG3, SUPT16H, VRK3, SP2, C1QBP, PARP2, TOP3A, CSNK2A2, XPC, RANBP17, PARP1, SUPV3L1, HIST2H2AC, HIST2H3PS2, KPNA4, USP7,


check button Protein-protein interactors based on sequence similarity (STRING)
GeneSTRING network
HMGA2all structure
WIF1all structure


check button - Retained interactions in fusion protein (protein functional feature from UniProt).
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost interactions due to fusion (protein functional feature from UniProt).
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with
HgeneHMGA2chr12:66345196chr12:65445253ENST00000354636+1444_630107.0E4F1
HgeneHMGA2chr12:66345196chr12:65445253ENST00000393578+1444_63091.0E4F1
HgeneHMGA2chr12:66345196chr12:65445253ENST00000425208+1444_63093.0E4F1


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Related Drugs to HMGA2-WIF1


check button Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDrugSourcePMID

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Related Diseases to HMGA2-WIF1


check button Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDiseaseSourcePMID

check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource
HgeneHMGA2C1519176Salivary Gland Pleomorphic Adenoma2ORPHANET
HgeneHMGA2C0005612Birth Weight1CTD_human
HgeneHMGA2C0006826Malignant Neoplasms1CTD_human
HgeneHMGA2C0027626Neoplasm Invasiveness1CTD_human
HgeneHMGA2C0027651Neoplasms1CTD_human
HgeneHMGA2C0086692Benign Neoplasm1CTD_human
HgeneHMGA2C0175693Russell-Silver syndrome1GENOMICS_ENGLAND
HgeneHMGA2C0473935Radiolabeled somatostatin analog study1GENOMICS_ENGLAND
HgeneHMGA2C0796160MENTAL RETARDATION, X-LINKED, SNYDER-ROBINSON TYPE1GENOMICS_ENGLAND
HgeneHMGA2C1096309Myolipoma1GENOMICS_ENGLAND
HgeneHMGA2C430514012q14 microdeletion syndrome1ORPHANET
TgeneWIF1C0009402Colorectal Carcinoma1CTD_human
TgeneWIF1C0009404Colorectal Neoplasms1CTD_human
TgeneWIF1C0014859Esophageal Neoplasms1CTD_human
TgeneWIF1C0024623Malignant neoplasm of stomach1CTD_human
TgeneWIF1C0027439Nasopharyngeal Neoplasms1CTD_human
TgeneWIF1C0038356Stomach Neoplasms1CTD_human
TgeneWIF1C0238301Cancer of Nasopharynx1CTD_human
TgeneWIF1C0546837Malignant neoplasm of esophagus1CTD_human
TgeneWIF1C1708349Hereditary Diffuse Gastric Cancer1CTD_human