UTHEALTH HOME    ABOUT SBMI    A-Z    WEBMAIL    INSIDE THE UNIVERSITY
FusionGDB Logo

Home

Download

Statistics

Examples

Help

Contact

Terms of Use

Center for Computational Systems Medicine level1
leaf

Fusion Gene Summary

leaf

Fusion Gene Sample Information

leaf

Fusion ORF Analysis

leaf

Fusion Amino Acid Sequences

leaf

Fusion Protein Functional Features

leaf

Fusion Protein-Protein Interaction

leaf

Related drugs with this fusion protein

leaf

Related disease with this fusion protein

Fusion Protein:HNRNPM-EEF2K

Fusion Protein Summary

check button Fusion gene summary
Fusion partner gene informationFusion gene name: HNRNPM-EEF2K
FusionPDB ID: 37274
FusionGDB2.0 ID: 37274
HgeneTgene
Gene symbol

HNRNPM

EEF2K

Gene ID

4670

29904

Gene nameheterogeneous nuclear ribonucleoprotein Meukaryotic elongation factor 2 kinase
SynonymsCEAR|HNRNPM4|HNRPM|HNRPM4|HTGR1|NAGR1|hnRNP MCaMKIII|HSU93850|eEF-2K
Cytomap

19p13.2

16p12.2

Type of geneprotein-codingprotein-coding
Descriptionheterogeneous nuclear ribonucleoprotein MCEA receptorN-acetylglucosamine receptor 1heterogenous nuclear ribonucleoprotein M4hnRNA-binding protein M4eukaryotic elongation factor 2 kinasealternative protein EEF2Kcalcium/calmodulin-dependent eukaryotic elongation factor-2 kinasecalmodulin-dependent protein kinase IIIeEF-2 kinaseelongation factor-2 kinaseeukaroytic elongation factor 2 kinase
Modification date2020032220200313
UniProtAcc

P52272

Q96G04

Ensembl transtripts involved in fusion geneENST idsENST00000325495, ENST00000348943, 
ENST00000602219, 
ENST00000561791, 
ENST00000263026, 
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)* DoF score16 X 12 X 10=19203 X 3 X 3=27
# samples 193
** MAII scorelog2(19/1920*10)=-3.33703498727757
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
log2(3/27*10)=0.15200309344505
effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs).
DoF>8 and MAII>0
Context (manual curation of fusion genes in FusionPDB)

PubMed: HNRNPM [Title/Abstract] AND EEF2K [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)HNRNPM(8532468)-EEF2K(22284947), # samples:1
Anticipated loss of major functional domain due to fusion event.HNRNPM-EEF2K seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
HNRNPM-EEF2K seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
HNRNPM-EEF2K seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
HNRNPM-EEF2K seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
TgeneEEF2K

GO:0046777

protein autophosphorylation

9144159|23184662


check buttonFusion gene breakpoints across HNRNPM (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check buttonFusion gene breakpoints across EEF2K (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure


Top

Fusion Gene Sample Information

check buttonFusion gene information from FusionGDB2.0.
check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4STADTCGA-CD-8531-01AHNRNPMchr19

8532468

+EEF2Kchr16

22284947

+


Top

Fusion ORF Analysis


check buttonFusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)
BP loci
(transcript)
Predicted start
(transcript)
Predicted stop
(transcript)
Seq length
(amino acids)
ENST00000348943HNRNPMchr198532468+ENST00000263026EEF2Kchr1622284947+40009491511362403
ENST00000325495HNRNPMchr198532468+ENST00000263026EEF2Kchr1622284947+3926875411288415

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000348943ENST00000263026HNRNPMchr198532468+EEF2Kchr1622284947+0.0003065090.9996935
ENST00000325495ENST00000263026HNRNPMchr198532468+EEF2Kchr1622284947+0.0003579850.999642

Top

Fusion Amino Acid Sequences


check button For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>37274_37274_1_HNRNPM-EEF2K_HNRNPM_chr19_8532468_ENST00000325495_EEF2K_chr16_22284947_ENST00000263026_length(amino acids)=415AA_BP=278
MAAGVEAAAEVAATEIKMEEESGAPGVPSGNGAPGPKGEGERPAQNEKRKEKNIKRGGNRFEPYANPTKRYRAFITNIPFDVKWQSLKDL
VKEKVGEVTYVELLMDAEGKSRGCAVVEFKMEESMKKAAEVLNKHSLSGRPLKVKEDPDGEHARRAMQKVMATTGGMGMGPGGPGMITIP
PSILNNPNIPNEIIHALQAGRLGSTVFVANLDYKVGWKKLKEVFSMAGVVVRADILEDKDGKSRGIGTVTFEQSIEAVQAISMFNGQLLF
DRPMHVKMETEENKTKGFDYLLKAAEAGDRQSMILVARAFDSGQNLSPDRCQDWLEALHWYNTALEMTDCDEGGEYDGMQDEPRYMMLAR

--------------------------------------------------------------

>37274_37274_2_HNRNPM-EEF2K_HNRNPM_chr19_8532468_ENST00000348943_EEF2K_chr16_22284947_ENST00000263026_length(amino acids)=403AA_BP=266
MVASGHVGAQAQRGAARSLTQSPDAEKMAAGVEAAAEVAATEIKMEEESGAPGVPSGNGAPGPKGEGERPAQNEKRKEKNIKRGGNRFEP
YANPTKRYRAFITNIPFDVKWQSLKDLVKEKVGEVTYVELLMDAEGKSRGCAVVEFKMEESMKKAAEVLNKHSLSGRPLKVKEDPDGEHA
RRAMQKAGRLGSTVFVANLDYKVGWKKLKEVFSMAGVVVRADILEDKDGKSRGIGTVTFEQSIEAVQAISMFNGQLLFDRPMHVKMETEE
NKTKGFDYLLKAAEAGDRQSMILVARAFDSGQNLSPDRCQDWLEALHWYNTALEMTDCDEGGEYDGMQDEPRYMMLAREAEMLFTGGYGL

--------------------------------------------------------------

Top

Fusion Protein Functional Features


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr19:8532468/chr16:22284947)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
HNRNPM

P52272

EEF2K

Q96G04

FUNCTION: Pre-mRNA binding protein in vivo, binds avidly to poly(G) and poly(U) RNA homopolymers in vitro. Involved in splicing. Acts as a receptor for carcinoembryonic antigen in Kupffer cells, may initiate a series of signaling events leading to tyrosine phosphorylation of proteins and induction of IL-1 alpha, IL-6, IL-10 and tumor necrosis factor alpha cytokines.FUNCTION: Catalyzes the trimethylation of eukaryotic elongation factor 2 (EEF2) on 'Lys-525'. {ECO:0000269|PubMed:25231979}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page

* Minus value of BPloci means that the break pointn is located before the CDS.
- Retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000325495+816204_281278.0731.0DomainRRM 2
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000325495+81671_149278.0731.0DomainRRM 1
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000348943+91771_149239.0692.0DomainRRM 1

- Not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000325495+816390_396278.0731.0Compositional biasNote=Poly-Gly
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000325495+816612_616278.0731.0Compositional biasNote=Poly-Gly
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000348943+917390_396239.0692.0Compositional biasNote=Poly-Gly
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000348943+917612_616239.0692.0Compositional biasNote=Poly-Gly
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000325495+816653_729278.0731.0DomainRRM 3
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000348943+917204_281239.0692.0DomainRRM 2
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000348943+917653_729239.0692.0DomainRRM 3
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000325495+816400_608278.0731.0RegionNote=27 X 6 AA repeats of [GEVSTPAN]-[ILMV]-[DE]-[RH]-[MLVI]-[GAV]
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000348943+917400_608239.0692.0RegionNote=27 X 6 AA repeats of [GEVSTPAN]-[ILMV]-[DE]-[RH]-[MLVI]-[GAV]
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000325495+816400_405278.0731.0RepeatNote=1
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000325495+816407_412278.0731.0RepeatNote=2
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000325495+816415_420278.0731.0RepeatNote=3
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000325495+816426_431278.0731.0RepeatNote=4
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000325495+816433_438278.0731.0RepeatNote=5
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000325495+816440_445278.0731.0RepeatNote=6
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000325495+816446_451278.0731.0RepeatNote=7
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000325495+816453_458278.0731.0RepeatNote=8
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000325495+816461_466278.0731.0RepeatNote=9
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000325495+816468_473278.0731.0RepeatNote=10
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000325495+816475_480278.0731.0RepeatNote=11
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000325495+816482_487278.0731.0RepeatNote=12
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000325495+816493_498278.0731.0RepeatNote=13
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000325495+816500_505278.0731.0RepeatNote=14
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000325495+816507_512278.0731.0RepeatNote=15
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000325495+816514_519278.0731.0RepeatNote=16
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000325495+816521_526278.0731.0RepeatNote=17
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000325495+816528_533278.0731.0RepeatNote=18
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000325495+816540_545278.0731.0RepeatNote=19
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000325495+816547_552278.0731.0RepeatNote=20
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000325495+816554_559278.0731.0RepeatNote=21
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000325495+816562_566278.0731.0RepeatNote=22
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000325495+816567_572278.0731.0RepeatNote=23
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000325495+816575_579278.0731.0RepeatNote=24
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000325495+816580_585278.0731.0RepeatNote=25
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000325495+816588_593278.0731.0RepeatNote=26
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000325495+816603_608278.0731.0RepeatNote=27
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000348943+917400_405239.0692.0RepeatNote=1
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000348943+917407_412239.0692.0RepeatNote=2
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000348943+917415_420239.0692.0RepeatNote=3
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000348943+917426_431239.0692.0RepeatNote=4
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000348943+917433_438239.0692.0RepeatNote=5
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000348943+917440_445239.0692.0RepeatNote=6
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000348943+917446_451239.0692.0RepeatNote=7
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000348943+917453_458239.0692.0RepeatNote=8
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000348943+917461_466239.0692.0RepeatNote=9
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000348943+917468_473239.0692.0RepeatNote=10
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000348943+917475_480239.0692.0RepeatNote=11
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000348943+917482_487239.0692.0RepeatNote=12
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000348943+917493_498239.0692.0RepeatNote=13
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000348943+917500_505239.0692.0RepeatNote=14
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000348943+917507_512239.0692.0RepeatNote=15
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000348943+917514_519239.0692.0RepeatNote=16
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000348943+917521_526239.0692.0RepeatNote=17
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000348943+917528_533239.0692.0RepeatNote=18
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000348943+917540_545239.0692.0RepeatNote=19
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000348943+917547_552239.0692.0RepeatNote=20
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000348943+917554_559239.0692.0RepeatNote=21
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000348943+917562_566239.0692.0RepeatNote=22
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000348943+917567_572239.0692.0RepeatNote=23
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000348943+917575_579239.0692.0RepeatNote=24
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000348943+917580_585239.0692.0RepeatNote=25
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000348943+917588_593239.0692.0RepeatNote=26
HgeneHNRNPMchr19:8532468chr16:22284947ENST00000348943+917603_608239.0692.0RepeatNote=27
TgeneEEF2Kchr19:8532468chr16:22284947ENST000002630261418116_326588.0726.0DomainAlpha-type protein kinase
TgeneEEF2Kchr19:8532468chr16:22284947ENST000002630261418296_302588.0726.0Nucleotide bindingATP
TgeneEEF2Kchr19:8532468chr16:22284947ENST00000263026141881_94588.0726.0RegionCalmodulin-binding


Top

Fusion Protein-Protein Interaction


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)
GenePPI interactors


check button Protein-protein interactors based on sequence similarity (STRING)
GeneSTRING network
HNRNPM
EEF2K


check button - Retained interactions in fusion protein (protein functional feature from UniProt).
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost interactions due to fusion (protein functional feature from UniProt).
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


Top

Related Drugs to HNRNPM-EEF2K


check button Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDrugSourcePMID

Top

Related Diseases to HNRNPM-EEF2K


check button Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDiseaseSourcePMID

check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource