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Center for Computational Systems Medicine level3
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Fusion Gene Summary

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Fusion Gene Sample Information

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Fusion ORF Analysis

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Fusion Amino Acid Sequences

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Fusion Protein Functional Features

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Fusion Protein Structure

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pLDDT scores

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Ramachandran Plot of Fusion Protein Structure

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Potential Active Site Information

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Potentially Interacting Small Molecules through Virtual Screening

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Biochemical Features of Small Molecules with ADME

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Drug Toxicity Information

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Fusion Protein-Protein Interaction

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Related drugs with this fusion protein

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Related disease with this fusion protein

Fusion Protein:HOOK3-RET

Fusion Protein Summary

check button Fusion gene summary
Fusion partner gene informationFusion gene name: HOOK3-RET
FusionPDB ID: 37395
FusionGDB2.0 ID: 37395
HgeneTgene
Gene symbol

HOOK3

RET

Gene ID

84376

5979

Gene namehook microtubule tethering protein 3ret proto-oncogene
SynonymsHK3CDHF12|CDHR16|HSCR1|MEN2A|MEN2B|MTC1|PTC|RET-ELE1
Cytomap

8p11.21

10q11.21

Type of geneprotein-codingprotein-coding
Descriptionprotein Hook homolog 3h-hook3hHK3hook homolog 3proto-oncogene tyrosine-protein kinase receptor RetRET receptor tyrosine kinasecadherin family member 12cadherin-related family member 16proto-oncogene c-Retrearranged during transfectionret proto-oncogene (multiple endocrine neoplasia and medullary
Modification date2020031320200322
UniProtAcc

Q86VS8

RTL1

Ensembl transtripts involved in fusion geneENST idsENST00000307602, ENST00000524839, 
ENST00000340058, ENST00000355710, 
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)* DoF score16 X 13 X 9=187232 X 31 X 11=10912
# samples 1948
** MAII scorelog2(19/1872*10)=-3.30050911125246
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(48/10912*10)=-4.50673733341565
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)

PubMed: HOOK3 [Title/Abstract] AND RET [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)HOOK3(42823358)-RET(43612030), # samples:2
Anticipated loss of major functional domain due to fusion event.HOOK3-RET seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
HOOK3-RET seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
HOOK3-RET seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
HOOK3-RET seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
TgeneRET

GO:0030155

regulation of cell adhesion

21357690

TgeneRET

GO:0030335

positive regulation of cell migration

20702524

TgeneRET

GO:0033619

membrane protein proteolysis

21357690

TgeneRET

GO:0033630

positive regulation of cell adhesion mediated by integrin

20702524

TgeneRET

GO:0035860

glial cell-derived neurotrophic factor receptor signaling pathway

28953886

TgeneRET

GO:0043410

positive regulation of MAPK cascade

28846099


check buttonFusion gene breakpoints across HOOK3 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check buttonFusion gene breakpoints across RET (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure


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Fusion Gene Sample Information

check buttonFusion gene information from FusionGDB2.0.
check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4papilary thyroid carcinomaDQ104207HOOK3chr8

42823358

RETchr10

43612030
ChimerKB3..HOOK3chr8

42823357

+RETchr10

43612031

+
ChimerKB3..HOOK3chr8

42823358

+RETchr10

43612030

+
ChiTaRS5.0N/ADQ104207HOOK3chr8

42823358

+RETchr10

43612030

+


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Fusion ORF Analysis


check buttonFusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000307602HOOK3chr842823357+ENST00000355710RETchr1043612031+461313221822530782
ENST00000307602HOOK3chr842823357+ENST00000340058RETchr1043612031+315513221822404740
ENST00000307602HOOK3chr842823358+ENST00000355710RETchr1043612030+461313221822530782
ENST00000307602HOOK3chr842823358+ENST00000340058RETchr1043612030+315513221822404740
ENST00000307602HOOK3chr842823358ENST00000355710RETchr1043612030461313221822530782
ENST00000307602HOOK3chr842823358ENST00000340058RETchr1043612030315513221822404740

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000307602ENST00000355710HOOK3chr842823358RETchr10436120300.000751780.9992482
ENST00000307602ENST00000340058HOOK3chr842823358RETchr10436120300.0044130480.995587

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Fusion Amino Acid Sequences


check button For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>37395_37395_1_HOOK3-RET_HOOK3_chr8_42823357_ENST00000307602_RET_chr10_43612031_ENST00000340058_length(amino acids)=740AA_BP=380
MAAAGKMFSVESLERAELCESLLTWIQTFNVDAPCQTVEDLTNGVVMAQVLQKIDPAYFDENWLNRIKTEVGDNWRLKISNLKKILKGIL
DYNHEILGQQINDFTLPDVNLIGEHSDAAELGRMLQLILGCAVNCEQKQEYIQAIMMMEESVQHVVMTAIQELMSKESPVSAGNDAYVDL
DRQLKKTTEELNEALSAKEEIAQRCHELDMQVAALQEEKSSLLAENQVLMERLNQSDSIEDPNSPAGRRHLQLQTQLEQLQEETFRLEAA
KDDYRIRCEELEKEISELRQQNDELTTLADEAQSLKDEIDVLRHSSDKVSKLEGQVESYKKKLEDLGDLRRQVKLLEEKNTMYMQNTVSL
EEELRKANAARSQLETYKRQEDPKWEFPRKNLVLGKTLGEGEFGKVVKATAFHLKGRAGYTTVAVKMLKENASPSELRDLLSEFNVLKQV
NHPHVIKLYGACSQDGPLLLIVEYAKYGSLRGFLRESRKVGPGYLGSGGSRNSSSLDHPDERALTMGDLISFAWQISQGMQYLAEMKLVH
RDLAARNILVAEGRKMKISDFGLSRDVYEEDSYVKRSQGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPERLFN
LLKTGHRMERPDNCSEEMYRLMLQCWKQEPDKRPVFADISKDLEKMMVKRRDYLDLAASTPSDSLIYDDGLSEEETPLVDCNNAPLPRAL

--------------------------------------------------------------

>37395_37395_2_HOOK3-RET_HOOK3_chr8_42823357_ENST00000307602_RET_chr10_43612031_ENST00000355710_length(amino acids)=782AA_BP=380
MAAAGKMFSVESLERAELCESLLTWIQTFNVDAPCQTVEDLTNGVVMAQVLQKIDPAYFDENWLNRIKTEVGDNWRLKISNLKKILKGIL
DYNHEILGQQINDFTLPDVNLIGEHSDAAELGRMLQLILGCAVNCEQKQEYIQAIMMMEESVQHVVMTAIQELMSKESPVSAGNDAYVDL
DRQLKKTTEELNEALSAKEEIAQRCHELDMQVAALQEEKSSLLAENQVLMERLNQSDSIEDPNSPAGRRHLQLQTQLEQLQEETFRLEAA
KDDYRIRCEELEKEISELRQQNDELTTLADEAQSLKDEIDVLRHSSDKVSKLEGQVESYKKKLEDLGDLRRQVKLLEEKNTMYMQNTVSL
EEELRKANAARSQLETYKRQEDPKWEFPRKNLVLGKTLGEGEFGKVVKATAFHLKGRAGYTTVAVKMLKENASPSELRDLLSEFNVLKQV
NHPHVIKLYGACSQDGPLLLIVEYAKYGSLRGFLRESRKVGPGYLGSGGSRNSSSLDHPDERALTMGDLISFAWQISQGMQYLAEMKLVH
RDLAARNILVAEGRKMKISDFGLSRDVYEEDSYVKRSQGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPERLFN
LLKTGHRMERPDNCSEEMYRLMLQCWKQEPDKRPVFADISKDLEKMMVKRRDYLDLAASTPSDSLIYDDGLSEEETPLVDCNNAPLPRAL

--------------------------------------------------------------

>37395_37395_3_HOOK3-RET_HOOK3_chr8_42823358_ENST00000307602_RET_chr10_43612030_ENST00000340058_length(amino acids)=740AA_BP=380
MAAAGKMFSVESLERAELCESLLTWIQTFNVDAPCQTVEDLTNGVVMAQVLQKIDPAYFDENWLNRIKTEVGDNWRLKISNLKKILKGIL
DYNHEILGQQINDFTLPDVNLIGEHSDAAELGRMLQLILGCAVNCEQKQEYIQAIMMMEESVQHVVMTAIQELMSKESPVSAGNDAYVDL
DRQLKKTTEELNEALSAKEEIAQRCHELDMQVAALQEEKSSLLAENQVLMERLNQSDSIEDPNSPAGRRHLQLQTQLEQLQEETFRLEAA
KDDYRIRCEELEKEISELRQQNDELTTLADEAQSLKDEIDVLRHSSDKVSKLEGQVESYKKKLEDLGDLRRQVKLLEEKNTMYMQNTVSL
EEELRKANAARSQLETYKRQEDPKWEFPRKNLVLGKTLGEGEFGKVVKATAFHLKGRAGYTTVAVKMLKENASPSELRDLLSEFNVLKQV
NHPHVIKLYGACSQDGPLLLIVEYAKYGSLRGFLRESRKVGPGYLGSGGSRNSSSLDHPDERALTMGDLISFAWQISQGMQYLAEMKLVH
RDLAARNILVAEGRKMKISDFGLSRDVYEEDSYVKRSQGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPERLFN
LLKTGHRMERPDNCSEEMYRLMLQCWKQEPDKRPVFADISKDLEKMMVKRRDYLDLAASTPSDSLIYDDGLSEEETPLVDCNNAPLPRAL

--------------------------------------------------------------

>37395_37395_4_HOOK3-RET_HOOK3_chr8_42823358_ENST00000307602_RET_chr10_43612030_ENST00000355710_length(amino acids)=782AA_BP=380
MAAAGKMFSVESLERAELCESLLTWIQTFNVDAPCQTVEDLTNGVVMAQVLQKIDPAYFDENWLNRIKTEVGDNWRLKISNLKKILKGIL
DYNHEILGQQINDFTLPDVNLIGEHSDAAELGRMLQLILGCAVNCEQKQEYIQAIMMMEESVQHVVMTAIQELMSKESPVSAGNDAYVDL
DRQLKKTTEELNEALSAKEEIAQRCHELDMQVAALQEEKSSLLAENQVLMERLNQSDSIEDPNSPAGRRHLQLQTQLEQLQEETFRLEAA
KDDYRIRCEELEKEISELRQQNDELTTLADEAQSLKDEIDVLRHSSDKVSKLEGQVESYKKKLEDLGDLRRQVKLLEEKNTMYMQNTVSL
EEELRKANAARSQLETYKRQEDPKWEFPRKNLVLGKTLGEGEFGKVVKATAFHLKGRAGYTTVAVKMLKENASPSELRDLLSEFNVLKQV
NHPHVIKLYGACSQDGPLLLIVEYAKYGSLRGFLRESRKVGPGYLGSGGSRNSSSLDHPDERALTMGDLISFAWQISQGMQYLAEMKLVH
RDLAARNILVAEGRKMKISDFGLSRDVYEEDSYVKRSQGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPERLFN
LLKTGHRMERPDNCSEEMYRLMLQCWKQEPDKRPVFADISKDLEKMMVKRRDYLDLAASTPSDSLIYDDGLSEEETPLVDCNNAPLPRAL

--------------------------------------------------------------

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Fusion Protein Functional Features


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr8:42823358/chr10:43612030)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
HOOK3

Q86VS8

RET

RTL1

FUNCTION: Probably serves as a target for the spiC protein from Salmonella typhimurium, which inactivates it, leading to a strong alteration in cellular trafficking (By similarity). Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). May regulate clearance of endocytosed receptors such as MSR1. Participates in defining the architecture and localization of the Golgi complex. Acts as an adapter protein linking the dynein motor complex to various cargos and converts dynein from a non-processive to a highly processive motor in the presence of dynactin. Facilitates the interaction between dynein and dynactin and activates dynein processivity (the ability to move along a microtubule for a long distance without falling off the track) (PubMed:25035494). FHF complex promotes the distribution of AP-4 complex to the perinuclear area of the cell (PubMed:32073997). {ECO:0000250|UniProtKB:Q8BUK6, ECO:0000269|PubMed:11238449, ECO:0000269|PubMed:17237231, ECO:0000269|PubMed:18799622, ECO:0000269|PubMed:25035494, ECO:0000269|PubMed:32073997}.; FUNCTION: (Microbial infection) May serve as a target for the spiC protein from Salmonella typhimurium, which inactivates it, leading to a strong alteration in cellular trafficking. {ECO:0000305}.1358

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page

* Minus value of BPloci means that the break pointn is located before the CDS.
- Retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneHOOK3chr8:42823358chr10:43612030ENST00000307602+112210_126374.0719.0DomainCalponin-homology (CH)
TgeneRETchr8:42823358chr10:43612030ENST000003400581019724_1016712.01073.0DomainProtein kinase
TgeneRETchr8:42823358chr10:43612030ENST000003557101020724_1016712.01115.0DomainProtein kinase
TgeneRETchr8:42823358chr10:43612030ENST000003400581019730_738712.01073.0Nucleotide bindingATP
TgeneRETchr8:42823358chr10:43612030ENST000003557101020730_738712.01115.0Nucleotide bindingATP
TgeneRETchr8:42823358chr10:43612030ENST000003400581019805_807712.01073.0RegionNote=Inhibitors binding
TgeneRETchr8:42823358chr10:43612030ENST000003557101020805_807712.01115.0RegionNote=Inhibitors binding

- Not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneHOOK3chr8:42823358chr10:43612030ENST00000307602+1122167_433374.0719.0Coiled coilOntology_term=ECO:0000255
HgeneHOOK3chr8:42823358chr10:43612030ENST00000307602+1122462_667374.0719.0Coiled coilOntology_term=ECO:0000255
HgeneHOOK3chr8:42823358chr10:43612030ENST00000307602+1122553_718374.0719.0RegionNote=Required for association with Golgi
TgeneRETchr8:42823358chr10:43612030ENST000003400581019168_272712.01073.0DomainCadherin
TgeneRETchr8:42823358chr10:43612030ENST000003557101020168_272712.01115.0DomainCadherin
TgeneRETchr8:42823358chr10:43612030ENST00000340058101929_635712.01073.0Topological domainExtracellular
TgeneRETchr8:42823358chr10:43612030ENST000003400581019658_1114712.01073.0Topological domainCytoplasmic
TgeneRETchr8:42823358chr10:43612030ENST00000355710102029_635712.01115.0Topological domainExtracellular
TgeneRETchr8:42823358chr10:43612030ENST000003557101020658_1114712.01115.0Topological domainCytoplasmic
TgeneRETchr8:42823358chr10:43612030ENST000003400581019636_657712.01073.0TransmembraneHelical
TgeneRETchr8:42823358chr10:43612030ENST000003557101020636_657712.01115.0TransmembraneHelical


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Fusion Protein Structures

check button PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.
Fusion protein PDB link (fusion AA seq ID in FusionPDB)HgeneHchrHbpHstrandTgeneTchrTbpTstrandAA seqLen(AA seq)
PDB file (605) >>>605.pdbFusion protein BP residue: 380
CIF file (605) >>>605.cif		HOOK3chr842823357+RETchr1043612031+
MAAAGKMFSVESLERAELCESLLTWIQTFNVDAPCQTVEDLTNGVVMAQV
LQKIDPAYFDENWLNRIKTEVGDNWRLKISNLKKILKGILDYNHEILGQQ
INDFTLPDVNLIGEHSDAAELGRMLQLILGCAVNCEQKQEYIQAIMMMEE
SVQHVVMTAIQELMSKESPVSAGNDAYVDLDRQLKKTTEELNEALSAKEE
IAQRCHELDMQVAALQEEKSSLLAENQVLMERLNQSDSIEDPNSPAGRRH
LQLQTQLEQLQEETFRLEAAKDDYRIRCEELEKEISELRQQNDELTTLAD
EAQSLKDEIDVLRHSSDKVSKLEGQVESYKKKLEDLGDLRRQVKLLEEKN
TMYMQNTVSLEEELRKANAARSQLETYKRQEDPKWEFPRKNLVLGKTLGE
GEFGKVVKATAFHLKGRAGYTTVAVKMLKENASPSELRDLLSEFNVLKQV
NHPHVIKLYGACSQDGPLLLIVEYAKYGSLRGFLRESRKVGPGYLGSGGS
RNSSSLDHPDERALTMGDLISFAWQISQGMQYLAEMKLVHRDLAARNILV
AEGRKMKISDFGLSRDVYEEDSYVKRSQGRIPVKWMAIESLFDHIYTTQS
DVWSFGVLLWEIVTLGGNPYPGIPPERLFNLLKTGHRMERPDNCSEEMYR
LMLQCWKQEPDKRPVFADISKDLEKMMVKRRDYLDLAASTPSDSLIYDDG
740
3D view using mol* of 605 (AA BP:380)
PDB file (641) >>>641.pdbFusion protein BP residue: 380
CIF file (641) >>>641.cif		HOOK3chr842823357+RETchr1043612031+
MAAAGKMFSVESLERAELCESLLTWIQTFNVDAPCQTVEDLTNGVVMAQV
LQKIDPAYFDENWLNRIKTEVGDNWRLKISNLKKILKGILDYNHEILGQQ
INDFTLPDVNLIGEHSDAAELGRMLQLILGCAVNCEQKQEYIQAIMMMEE
SVQHVVMTAIQELMSKESPVSAGNDAYVDLDRQLKKTTEELNEALSAKEE
IAQRCHELDMQVAALQEEKSSLLAENQVLMERLNQSDSIEDPNSPAGRRH
LQLQTQLEQLQEETFRLEAAKDDYRIRCEELEKEISELRQQNDELTTLAD
EAQSLKDEIDVLRHSSDKVSKLEGQVESYKKKLEDLGDLRRQVKLLEEKN
TMYMQNTVSLEEELRKANAARSQLETYKRQEDPKWEFPRKNLVLGKTLGE
GEFGKVVKATAFHLKGRAGYTTVAVKMLKENASPSELRDLLSEFNVLKQV
NHPHVIKLYGACSQDGPLLLIVEYAKYGSLRGFLRESRKVGPGYLGSGGS
RNSSSLDHPDERALTMGDLISFAWQISQGMQYLAEMKLVHRDLAARNILV
AEGRKMKISDFGLSRDVYEEDSYVKRSQGRIPVKWMAIESLFDHIYTTQS
DVWSFGVLLWEIVTLGGNPYPGIPPERLFNLLKTGHRMERPDNCSEEMYR
LMLQCWKQEPDKRPVFADISKDLEKMMVKRRDYLDLAASTPSDSLIYDDG
LSEEETPLVDCNNAPLPRALPSTWIENKLYGMSDPNWPGESPVPLTRADG
782
3D view using mol* of 641 (AA BP:380)


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pLDDT score distribution

check button pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.
HOOK3_pLDDT.png
all structure
all structure
RET_pLDDT.png
all structure
all structure

check button pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.
HOOK3_RET_605_pLDDT.png (AA BP:380)
all structure
HOOK3_RET_605_pLDDT_and_active_sites.png (AA BP:380)
all structure
HOOK3_RET_605_violinplot.png (AA BP:380)
all structure
HOOK3_RET_641_pLDDT.png (AA BP:380)
all structure
HOOK3_RET_641_pLDDT_and_active_sites.png (AA BP:380)
all structure


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Ramachandran Plot of Fusion Protein Structure


check button Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.
Fusion AA seq ID in FusionPDB and their Ramachandran plots
HOOK3_RET_605.png
all structure
HOOK3_RET_641.png
all structure

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Potential Active Site Information


check button The potential binding sites of these fusion proteins were identified using SiteMap, a module of the Schrodinger suite.
Fusion AA seq ID in FusionPDBSite scoreSizeD scoreVolumeExposureEnclosureContactPhobicPhilicBalanceDon/AccResidues
6051.0811271.124302.1830.4410.7590.9621.4460.8341.7341.092Chain A: 398,399,400,401,403,404,406,424,426,443,4
47,456,472,473,474,475,476,478,479,546,547,549,559
,560

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Potentially Interacting Small Molecules through Virtual Screening


check button The FDA-approved small molecule library molecules were subjected to virtual screening using the Glide.
Fusion AA seq ID in FusionPDBZINC IDDrugBank IDDrug nameDocking scoreGlide gscore

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check button Drug information from DrugBank of the top 20 interacting small molecules.
ZINC IDDrugBank IDDrug nameDrug typeSMILESDrug group

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Biochemical Features of Small Molecules


check button ADME (Absorption, Distribution, Metabolism, and Excretion) of drugs using QikProp(v3.9)
ZINC IDmol_MWdipoleSASAFOSAFISAPISAWPSAvolumedonorHBaccptHBIPHuman Oral AbsorptionPercent Human Oral AbsorptionRule Of FiveRule Of Three


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Drug Toxicity Information


check button Toxicity information of individual drugs using eToxPred
ZINC IDSmileSurface AccessibilityToxicity


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Fusion Protein-Protein Interaction


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)
GenePPI interactors
HOOK3CUL3, AKTIP, HOOK1, HOOK2, HOOK3, FAM160A2, VPS16, VPS41, SCFD1, CCDC8, ZC4H2, COMTD1, TNFSF13B, IFT57, FNTB, MRE11A, NTRK1, SSX2IP, CEP63, Nedd1, Rab5c, Zbtb48, DUSP3, FAM160A1, MTMR4, SYCE3, RALBP1, CDC16, NCAPH2, KLHL10, KLHL20, MYO6, TGOLN2, DYNC1I2, DYNC1LI1, DYNC1LI2, DYNC1H1, DCTN1, BRPF1, RAB21, RCN2, TPM3, MYH10, HYOU1, ACOT9, KRT19, ACTG1, ACACA, ACACB, IARS2, MTHFD1L, ZNF326, RAD50, MCCC1, GFPT1, PCCB, AASS, MTPAP, TUBB2B, HLCS, YWHAQ, MCM5, DLD, MYL6, ABCB7, PC, PCNA, CP, PCCA, PATL1, HNRNPH1, KRI1, SNX2, RBM14, DLG5, FAM160B1, FAM160B2, KIF1C, KLC4, TBC1D23, KIF1B, WDR11, FAM91A1, KLC2, GOLGA5, TBKBP1, WDR62, PCM1, NFKB1, TPD52L2, CPD, KIAA0319L, CEP192, PPP6R2, AAK1, KIF5B, ATG2A, CAPZA1, FAM21A, CAPZB, CCDC88A, FAM21C, NAP1L4, TJP2, ANKRD52, HAUS5, ABI2, GPATCH1, ANKRD28, KIAA0196, BSDC1, RELA, APC, FAM120A, LUZP1, COBL, BCR, BACH2, ARHGEF11, CEP55, WASF2, SIPA1L3, PPP6R3, AFTPH, VPS45, CORO1B, BCCIP, WDR83, PHF8, NAP1L1, LTV1, ALMS1, NBN, GAPVD1, OSBPL3, PPP6C, IFT27, CGN, SYNRG, MAP7D3, HBS1L, CEP97, ZC3H7A, DVL2, STRN, IGF2R, TJP1, SPAG5, CC2D1A, PLEC, MAPT, MEOX2, CTIF, KRT13, KRT27, PNMA5, ORF3a, M, nsp4, nsp6, ORF6, ORF7a, ORF7b, nsp12, nsp2, nsp3, CSDE1, TP53, EBAG9, AP4E1, KRT37, SYCE1, ITGA8, KIAA0226, HAP1, TMEM74, PIPSL, KRT31, FAM9C, NUBP1, CD40, MAGEA8, DUSP16, GPR17, KRT34, LCTL, AGBL4, SKAP1, ANKRD22, FEM1A, WHAMMP3, FTL, TRIM52, ARHGEF10, PPARD, SDF4, GFAP, EIF4ENIF1, Eif3g,
RETSTAT3, DOK6, DOK5, SRC, SHC1, PTPRF, DOK2, DOK4, DOK1, GRB2, CRK, PLCG1, PDLIM7, GRB7, FRS2, NRTN, GFRA1, GRB10, IKBKG, MCRS1, CBL, CBLC, RET, HSP90AA1, NOTCH2NL, NOTCH3, AIP, EGFR, NTRK1, ZBTB48, HNRNPD, SORT1, SYNCRIP, SGTB, BAG6, HIST1H3A, KYNU, TXNL4A, ZCCHC8, ICE2, MAPK3, PTK2, MAPK1, PTPRR, DUSP26, Shc1, PIK3R1, FBXO7, NEDD4, SHANK2, LRRK1, PTK6, CTNNB1, SHC4, ERBB2, ADAM10, SPINT2, PCDH7, LRIG2, APP, GDNF, IGF1R, CLU, EPCAM, CDH2, TGFBR1, PTK7, FAT1, DHCR24, SMN1, PCDH19, NCSTN, CERS2, CHP1, FANCD2, ITM2C, SEL1L, PCDHGB5, CKB, NRP1, PLXNB2, NCK2, CTNND1, TULP3, PTPN1, NF2, CRKL, CAMLG, NUMB, BASP1, ROR2, NOTCH2, PTPN13, ANK3, RAB3GAP1, PEA15, LLGL1, GPRIN3, RAPH1, MARK2, CYFIP1, ARC, GPRIN1, ANKLE2, VRK2, MIB1, PHACTR4, UNC5B, TMEM57, SYAP1, FAF2, ERBB2IP, FAM129B, KIAA1715, PEAK1, VANGL2, BAIAP2, EPN1, NUMBL, PRKAR2A, GAB1, PTPRA, DDX39A, TIMP1, EPHB4, MANEA, SRY, RABAC1, LEMD3, TMEM214, PDZD8, GPR50, SMIM5, RNF149, SDF2L1, BMPR1A, PLEKHH3, ACVR2A,


check button Protein-protein interactors based on sequence similarity (STRING)
GeneSTRING network
HOOK3all structure
RETall structure


check button - Retained interactions in fusion protein (protein functional feature from UniProt).
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost interactions due to fusion (protein functional feature from UniProt).
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs to HOOK3-RET


check button Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDrugSourcePMID

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Related Diseases to HOOK3-RET


check button Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDiseaseSourcePMID
HOOK3RETLung AdenocarcinomaMyCancerGenome
HOOK3RETBreast Invasive Ductal CarcinomaMyCancerGenome
HOOK3RETThyroid Gland Papillary CarcinomaMyCancerGenome
HOOK3RETAdenocarcinoma Of Unknown PrimaryMyCancerGenome
HOOK3RETBladder Urothelial CarcinomaMyCancerGenome

check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource
HgeneHOOK3C0032460Polycystic Ovary Syndrome1CTD_human
HgeneHOOK3C1136382Sclerocystic Ovaries1CTD_human
TgeneRETC1833921Familial medullary thyroid carcinoma23CTD_human;GENOMICS_ENGLAND;ORPHANET;UNIPROT
TgeneRETC3888239HIRSCHSPRUNG DISEASE, SUSCEPTIBILITY TO, 116GENOMICS_ENGLAND;UNIPROT
TgeneRETC0025268Multiple Endocrine Neoplasia Type 2a15CTD_human;GENOMICS_ENGLAND;ORPHANET;UNIPROT
TgeneRETC1708353Hereditary Paraganglioma-Pheochromocytoma Syndrome12CLINGEN
TgeneRETC0025269Multiple Endocrine Neoplasia Type 2b10CTD_human;GENOMICS_ENGLAND;ORPHANET;UNIPROT
TgeneRETC0238463Papillary thyroid carcinoma3CTD_human;ORPHANET
TgeneRETC1275808Congenital central hypoventilation3CTD_human;GENOMICS_ENGLAND;UNIPROT
TgeneRETC1859049CCHS WITH HIRSCHSPRUNG DISEASE3CTD_human;ORPHANET
TgeneRETC0009402Colorectal Carcinoma2CTD_human;UNIPROT
TgeneRETC0009404Colorectal Neoplasms2CTD_human
TgeneRETC0019569Hirschsprung Disease2CTD_human
TgeneRETC0027662Multiple Endocrine Neoplasia2CTD_human;GENOMICS_ENGLAND
TgeneRETC0085758Aganglionosis, Colonic2CTD_human
TgeneRETC0266294Unilateral agenesis of kidney2ORPHANET
TgeneRETC1257840Aganglionosis, Rectosigmoid Colon2CTD_human
TgeneRETC3661523Congenital Intestinal Aganglionosis2CTD_human
TgeneRETC0006413Burkitt Lymphoma1CTD_human
TgeneRETC0031511Pheochromocytoma1CGI;CTD_human;GENOMICS_ENGLAND;UNIPROT
TgeneRETC0038220Status Epilepticus1CTD_human
TgeneRETC0040136Thyroid Neoplasm1CGI;CTD_human
TgeneRETC0151468Thyroid Gland Follicular Adenoma1CTD_human
TgeneRETC0206693Medullary carcinoma1CTD_human
TgeneRETC0238462Medullary carcinoma of thyroid1CGI;CTD_human
TgeneRETC0270823Petit mal status1CTD_human
TgeneRETC0311335Grand Mal Status Epilepticus1CTD_human
TgeneRETC0343640African Burkitt's lymphoma1CTD_human
TgeneRETC0393734Complex Partial Status Epilepticus1CTD_human
TgeneRETC0549473Thyroid carcinoma1CGI;CTD_human;UNIPROT
TgeneRETC0740340Amyloidosis, Familial1CTD_human
TgeneRETC0751522Status Epilepticus, Subclinical1CTD_human
TgeneRETC0751523Non-Convulsive Status Epilepticus1CTD_human
TgeneRETC0751524Simple Partial Status Epilepticus1CTD_human
TgeneRETC1257877Pheochromocytoma, Extra-Adrenal1CTD_human
TgeneRETC1609433Congenital absence of kidneys syndrome1CTD_human;GENOMICS_ENGLAND;ORPHANET
TgeneRETC3501843Nonmedullary Thyroid Carcinoma1CTD_human
TgeneRETC3501844Familial Nonmedullary Thyroid Cancer1CTD_human
TgeneRETC4721444Burkitt Leukemia1CTD_human