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Fusion Protein:KEAP1-EYA2 |
Fusion Protein Summary |
 Fusion gene summary |
| Fusion partner gene information | Fusion gene name: KEAP1-EYA2 | FusionPDB ID: 41939 | FusionGDB2.0 ID: 41939 | Hgene | Tgene | Gene symbol | KEAP1 | EYA2 | Gene ID | 9817 | 2139 |
| Gene name | kelch like ECH associated protein 1 | EYA transcriptional coactivator and phosphatase 2 | |
| Synonyms | INrf2|KLHL19 | EAB1 | |
| Cytomap | 19p13.2 | 20q13.12 | |
| Type of gene | protein-coding | protein-coding | |
| Description | kelch-like ECH-associated protein 1KEAP1 delta Ccytosolic inhibitor of Nrf2kelch-like family member 19kelch-like protein 19 | eyes absent homolog 2 | |
| Modification date | 20200327 | 20200313 | |
| UniProtAcc | Q14145 | O00167 | |
| Ensembl transtripts involved in fusion gene | ENST ids | ENST00000171111, ENST00000393623, ENST00000588024, | ENST00000317304, ENST00000497428, ENST00000327619, ENST00000357410, |
| Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0) | * DoF score | 5 X 5 X 5=125 | 15 X 15 X 10=2250 |
| # samples | 5 | 30 | |
| ** MAII score | log2(5/125*10)=-1.32192809488736 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | log2(30/2250*10)=-2.90689059560852 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | |
| Context (manual curation of fusion genes in FusionPDB) | PubMed: KEAP1 [Title/Abstract] AND EYA2 [Title/Abstract] AND fusion [Title/Abstract] | ||
| Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0) | KEAP1(10599868)-EYA2(45797787), # samples:1 | ||
| Anticipated loss of major functional domain due to fusion event. | KEAP1-EYA2 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. KEAP1-EYA2 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. KEAP1-EYA2 seems lost the major protein functional domain in Hgene partner, which is a CGC due to the frame-shifted ORF. KEAP1-EYA2 seems lost the major protein functional domain in Hgene partner, which is a epigenetic factor due to the frame-shifted ORF. KEAP1-EYA2 seems lost the major protein functional domain in Hgene partner, which is a IUPHAR drug target due to the frame-shifted ORF. KEAP1-EYA2 seems lost the major protein functional domain in Tgene partner, which is a epigenetic factor due to the frame-shifted ORF. | ||
| * DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
| Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
| Partner | Gene | GO ID | GO term | PubMed ID |
| Hgene | KEAP1 | GO:0006511 | ubiquitin-dependent protein catabolic process | 15601839 |
| Hgene | KEAP1 | GO:0010506 | regulation of autophagy | 20452972 |
| Hgene | KEAP1 | GO:0016567 | protein ubiquitination | 15601839|15983046 |
| Hgene | KEAP1 | GO:0034599 | cellular response to oxidative stress | 15601839 |
| Tgene | EYA2 | GO:0016576 | histone dephosphorylation | 19351884 |
| Fusion gene breakpoints across KEAP1 (5'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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| Fusion gene breakpoints across EYA2 (3'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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Fusion Gene Sample Information |
| Fusion gene information from FusionGDB2.0. |
| Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0) * All genome coordinats were lifted-over on hg19. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| Source | Disease | Sample | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| ChimerDB4 | LUAD | TCGA-49-AARE-01A | KEAP1 | chr19 | 10599868 | - | EYA2 | chr20 | 45797787 | + |
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Fusion ORF Analysis |
| Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
| ENST00000171111 | KEAP1 | chr19 | 10599868 | - | ENST00000357410 | EYA2 | chr20 | 45797787 | + | 3369 | 2256 | 527 | 2263 | 578 |
| ENST00000171111 | KEAP1 | chr19 | 10599868 | - | ENST00000327619 | EYA2 | chr20 | 45797787 | + | 3606 | 2256 | 527 | 2263 | 578 |
| DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
| ENST00000171111 | ENST00000357410 | KEAP1 | chr19 | 10599868 | - | EYA2 | chr20 | 45797787 | + | 0.003174054 | 0.99682593 |
| ENST00000171111 | ENST00000327619 | KEAP1 | chr19 | 10599868 | - | EYA2 | chr20 | 45797787 | + | 0.003265551 | 0.99673444 |
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Fusion Amino Acid Sequences |
| For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones. |
| >FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP >41939_41939_1_KEAP1-EYA2_KEAP1_chr19_10599868_ENST00000171111_EYA2_chr20_45797787_ENST00000327619_length(amino acids)=578AA_BP= MLIFWNPMQPDPRPSGAGACCRFLPLQSQCPEGAGDAVMYASTECKAEVTPSQHGNRTFSYTLEDHTKQAFGIMNELRLSQQLCDVTLQV KYQDAPAAQFMAHKVVLASSSPVFKAMFTNGLREQGMEVVSIEGIHPKVMERLIEFAYTASISMGEKCVLHVMNGAVMYQIDSVVRACSD FLVQQLDPSNAIGIANFAEQIGCVELHQRAREYIYMHFGEVAKQEEFFNLSHCQLVTLISRDDLNVRCESEVFHACINWVKYDCEQRRFY VQALLRAVRCHSLTPNFLQMQLQKCEILQSDSRCKDYLVKIFEELTLHKPTQVMPCRAPKVGRLIYTAGGYFRQSLSYLEAYNPSDGTWL RLADLQVPRSGLAGCVVGGLLYAVGGRNNSPDGNTDSSALDCYNPMTNQWSPCAPMSVPRNRIGVGVIDGHIYAVGGSHGCIHHNSVERY EPERDEWHLVAPMLTRRIGVGVAVLNRLLYAVGGFDGTNRLNSAECYYPERNEWRMITAMNTIRSGAGVCVLHNCIYAAGGYDGQDQLNS -------------------------------------------------------------- >41939_41939_2_KEAP1-EYA2_KEAP1_chr19_10599868_ENST00000171111_EYA2_chr20_45797787_ENST00000357410_length(amino acids)=578AA_BP= MLIFWNPMQPDPRPSGAGACCRFLPLQSQCPEGAGDAVMYASTECKAEVTPSQHGNRTFSYTLEDHTKQAFGIMNELRLSQQLCDVTLQV KYQDAPAAQFMAHKVVLASSSPVFKAMFTNGLREQGMEVVSIEGIHPKVMERLIEFAYTASISMGEKCVLHVMNGAVMYQIDSVVRACSD FLVQQLDPSNAIGIANFAEQIGCVELHQRAREYIYMHFGEVAKQEEFFNLSHCQLVTLISRDDLNVRCESEVFHACINWVKYDCEQRRFY VQALLRAVRCHSLTPNFLQMQLQKCEILQSDSRCKDYLVKIFEELTLHKPTQVMPCRAPKVGRLIYTAGGYFRQSLSYLEAYNPSDGTWL RLADLQVPRSGLAGCVVGGLLYAVGGRNNSPDGNTDSSALDCYNPMTNQWSPCAPMSVPRNRIGVGVIDGHIYAVGGSHGCIHHNSVERY EPERDEWHLVAPMLTRRIGVGVAVLNRLLYAVGGFDGTNRLNSAECYYPERNEWRMITAMNTIRSGAGVCVLHNCIYAAGGYDGQDQLNS -------------------------------------------------------------- |
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Fusion Protein Functional Features |
| Four levels of functional features of fusion genes Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr19:10599868/chr20:45797787) - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. - How to search 1. Put your fusion gene symbol. 2. Press the tab key until there will be shown the breakpoint information filled. 4. Go down and press 'Search' tab twice. 4. Go down to have the hyperlink of the search result. 5. Click the hyperlink. 6. See the FGviewer result for your fusion gene. |
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| Main function of each fusion partner protein. (from UniProt) |
| Hgene | Tgene |
| KEAP1 | EYA2 |
| FUNCTION: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that regulates the response to oxidative stress by targeting NFE2L2/NRF2 for ubiquitination (PubMed:14585973, PubMed:15379550, PubMed:15572695, PubMed:15983046, PubMed:15601839). KEAP1 acts as a key sensor of oxidative and electrophilic stress: in normal conditions, the BCR(KEAP1) complex mediates ubiquitination and degradation of NFE2L2/NRF2, a transcription factor regulating expression of many cytoprotective genes (PubMed:15601839, PubMed:16006525). In response to oxidative stress, different electrophile metabolites trigger non-enzymatic covalent modifications of highly reactive cysteine residues in KEAP1, leading to inactivate the ubiquitin ligase activity of the BCR(KEAP1) complex, promoting NFE2L2/NRF2 nuclear accumulation and expression of phase II detoxifying enzymes (PubMed:19489739, PubMed:16006525, PubMed:17127771, PubMed:18251510, PubMed:29590092). In response to selective autophagy, KEAP1 is sequestered in inclusion bodies following its interaction with SQSTM1/p62, leading to inactivation of the BCR(KEAP1) complex and activation of NFE2L2/NRF2 (PubMed:20452972). The BCR(KEAP1) complex also mediates ubiquitination of SQSTM1/p62, increasing SQSTM1/p62 sequestering activity and degradation (PubMed:28380357). The BCR(KEAP1) complex also targets BPTF and PGAM5 for ubiquitination and degradation by the proteasome (PubMed:15379550, PubMed:17046835). {ECO:0000269|PubMed:14585973, ECO:0000269|PubMed:15379550, ECO:0000269|PubMed:15572695, ECO:0000269|PubMed:15601839, ECO:0000269|PubMed:15983046, ECO:0000269|PubMed:16006525, ECO:0000269|PubMed:17046835, ECO:0000269|PubMed:17127771, ECO:0000269|PubMed:18251510, ECO:0000269|PubMed:19489739, ECO:0000269|PubMed:20452972, ECO:0000269|PubMed:28380357, ECO:0000269|PubMed:29590092}. | FUNCTION: Functions both as protein phosphatase and as transcriptional coactivator for SIX1, and probably also for SIX2, SIX4 and SIX5 (PubMed:12500905, PubMed:23435380). Tyrosine phosphatase that dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph) and promotes efficient DNA repair via the recruitment of DNA repair complexes containing MDC1. 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress (PubMed:19351884). Its function as histone phosphatase may contribute to its function in transcription regulation during organogenesis. Plays an important role in hypaxial muscle development together with SIX1 and DACH2; in this it is functionally redundant with EYA1 (PubMed:12500905). {ECO:0000269|PubMed:12500905, ECO:0000269|PubMed:19351884, ECO:0000269|PubMed:21706047, ECO:0000269|PubMed:23435380}. |
| Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at * Minus value of BPloci means that the break pointn is located before the CDS. |
| - Retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| Hgene | KEAP1 | chr19:10599868 | chr20:45797787 | ENST00000171111 | - | 5 | 6 | 184_286 | 569.3333333333334 | 625.0 | Domain | Note=BACK |
| Hgene | KEAP1 | chr19:10599868 | chr20:45797787 | ENST00000171111 | - | 5 | 6 | 77_149 | 569.3333333333334 | 625.0 | Domain | BTB |
| Hgene | KEAP1 | chr19:10599868 | chr20:45797787 | ENST00000393623 | - | 5 | 6 | 184_286 | 569.3333333333334 | 625.0 | Domain | Note=BACK |
| Hgene | KEAP1 | chr19:10599868 | chr20:45797787 | ENST00000393623 | - | 5 | 6 | 77_149 | 569.3333333333334 | 625.0 | Domain | BTB |
| Hgene | KEAP1 | chr19:10599868 | chr20:45797787 | ENST00000171111 | - | 5 | 6 | 327_372 | 569.3333333333334 | 625.0 | Repeat | Note=Kelch 1 |
| Hgene | KEAP1 | chr19:10599868 | chr20:45797787 | ENST00000171111 | - | 5 | 6 | 373_423 | 569.3333333333334 | 625.0 | Repeat | Note=Kelch 2 |
| Hgene | KEAP1 | chr19:10599868 | chr20:45797787 | ENST00000171111 | - | 5 | 6 | 424_470 | 569.3333333333334 | 625.0 | Repeat | Note=Kelch 3 |
| Hgene | KEAP1 | chr19:10599868 | chr20:45797787 | ENST00000171111 | - | 5 | 6 | 471_517 | 569.3333333333334 | 625.0 | Repeat | Note=Kelch 4 |
| Hgene | KEAP1 | chr19:10599868 | chr20:45797787 | ENST00000171111 | - | 5 | 6 | 518_564 | 569.3333333333334 | 625.0 | Repeat | Note=Kelch 5 |
| Hgene | KEAP1 | chr19:10599868 | chr20:45797787 | ENST00000393623 | - | 5 | 6 | 327_372 | 569.3333333333334 | 625.0 | Repeat | Note=Kelch 1 |
| Hgene | KEAP1 | chr19:10599868 | chr20:45797787 | ENST00000393623 | - | 5 | 6 | 373_423 | 569.3333333333334 | 625.0 | Repeat | Note=Kelch 2 |
| Hgene | KEAP1 | chr19:10599868 | chr20:45797787 | ENST00000393623 | - | 5 | 6 | 424_470 | 569.3333333333334 | 625.0 | Repeat | Note=Kelch 3 |
| Hgene | KEAP1 | chr19:10599868 | chr20:45797787 | ENST00000393623 | - | 5 | 6 | 471_517 | 569.3333333333334 | 625.0 | Repeat | Note=Kelch 4 |
| Hgene | KEAP1 | chr19:10599868 | chr20:45797787 | ENST00000393623 | - | 5 | 6 | 518_564 | 569.3333333333334 | 625.0 | Repeat | Note=Kelch 5 |
| - Not-retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| Hgene | KEAP1 | chr19:10599868 | chr20:45797787 | ENST00000171111 | - | 5 | 6 | 565_611 | 569.3333333333334 | 625.0 | Repeat | Note=Kelch 6 |
| Hgene | KEAP1 | chr19:10599868 | chr20:45797787 | ENST00000393623 | - | 5 | 6 | 565_611 | 569.3333333333334 | 625.0 | Repeat | Note=Kelch 6 |
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Fusion Protein-Protein Interaction |
| Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in |
| Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160) |
| Gene | PPI interactors |
| Protein-protein interactors based on sequence similarity (STRING) |
| Gene | STRING network |
| KEAP1 | |
| EYA2 |
| - Retained interactions in fusion protein (protein functional feature from UniProt). |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Still interaction with |
| - Lost interactions due to fusion (protein functional feature from UniProt). |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
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Related Drugs to KEAP1-EYA2 |
| Drugs used for this fusion-positive patient. (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
| Hgene | Tgene | Drug | Source | PMID |
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Related Diseases to KEAP1-EYA2 |
| Diseases that have this fusion gene. (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
| Hgene | Tgene | Disease | Source | PMID |
| Diseases associated with fusion partners. (DisGeNet 4.0) |
| Partner | Gene | Disease ID | Disease name | # pubmeds | Source |
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