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Center for Computational Systems Medicine level2
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Fusion Gene Summary

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Fusion Gene Sample Information

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Fusion ORF Analysis

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Fusion Amino Acid Sequences

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Fusion Protein Functional Features

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Fusion Protein Structure

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pLDDT scores

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Ramachandran Plot of Fusion Protein Structure

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Fusion Protein-Protein Interaction

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Related drugs with this fusion protein

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Related disease with this fusion protein

Fusion Protein:AMFR-ITGAM

Fusion Protein Summary

check button Fusion gene summary
Fusion partner gene informationFusion gene name: AMFR-ITGAM
FusionPDB ID: 4216
FusionGDB2.0 ID: 4216
HgeneTgene
Gene symbol

AMFR

ITGAM

Gene ID

267

3684

Gene nameautocrine motility factor receptorintegrin subunit alpha M
SynonymsGP78|RNF45CD11B|CR3A|MAC-1|MAC1A|MO1A|SLEB6
Cytomap

16q13

16p11.2

Type of geneprotein-codingprotein-coding
DescriptionE3 ubiquitin-protein ligase AMFRRING finger protein 45RING-type E3 ubiquitin transferase AMFRintegrin alpha-MCD11 antigen-like family member BCR-3 alpha chainantigen CD11b (p170)cell surface glycoprotein MAC-1 subunit alphacomplement component 3 receptor 3 subunitintegrin, alpha M (complement component 3 receptor 3 subunit)leukocyte adhesi
Modification date2020032720200313
UniProtAcc

Q9UKV5

P11215

Ensembl transtripts involved in fusion geneENST idsENST00000290649, ENST00000564283, 
ENST00000287497, ENST00000544665, 
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)* DoF score7 X 5 X 5=1754 X 5 X 4=80
# samples 85
** MAII scorelog2(8/175*10)=-1.12928301694497
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(5/80*10)=-0.678071905112638
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)

PubMed: AMFR [Title/Abstract] AND ITGAM [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)AMFR(56419831)-ITGAM(31332562), # samples:3
Anticipated loss of major functional domain due to fusion event.AMFR-ITGAM seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
AMFR-ITGAM seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
AMFR-ITGAM seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
AMFR-ITGAM seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgeneAMFR

GO:0000209

protein polyubiquitination

17310145|19103148

HgeneAMFR

GO:0030433

ubiquitin-dependent ERAD pathway

11724934|17872946

HgeneAMFR

GO:0051865

protein autoubiquitination

24810856

HgeneAMFR

GO:0070936

protein K48-linked ubiquitination

12847084|24424410


check buttonFusion gene breakpoints across AMFR (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check buttonFusion gene breakpoints across ITGAM (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure


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Fusion Gene Sample Information

check buttonFusion gene information from FusionGDB2.0.
check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4BRCATCGA-D8-A27W-01AAMFRchr16

56419830

-ITGAMchr16

31332561

+
ChimerDB4BRCATCGA-D8-A27W-01AAMFRchr16

56419831

-ITGAMchr16

31332562

+
ChimerDB4BRCATCGA-D8-A27WAMFRchr16

56419830

-ITGAMchr16

31332561

+


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Fusion ORF Analysis


check buttonFusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000290649AMFRchr1656419831-ENST00000287497ITGAMchr1631332562+3404159121133421043
ENST00000290649AMFRchr1656419831-ENST00000544665ITGAMchr1631332562+4528159121133421043
ENST00000290649AMFRchr1656419830-ENST00000287497ITGAMchr1631332561+3404159121133421043
ENST00000290649AMFRchr1656419830-ENST00000544665ITGAMchr1631332561+4528159121133421043

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000290649ENST00000287497AMFRchr1656419831-ITGAMchr1631332562+0.0026156970.99738425
ENST00000290649ENST00000544665AMFRchr1656419831-ITGAMchr1631332562+0.001048530.99895144
ENST00000290649ENST00000287497AMFRchr1656419830-ITGAMchr1631332561+0.0026156970.99738425
ENST00000290649ENST00000544665AMFRchr1656419830-ITGAMchr1631332561+0.001048530.99895144

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Fusion Amino Acid Sequences


check button For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>4216_4216_1_AMFR-ITGAM_AMFR_chr16_56419830_ENST00000290649_ITGAM_chr16_31332561_ENST00000287497_length(amino acids)=1043AA_BP=460
MPLLFLERFPWPSLRTYTGLSGLALLGTIISAYRALSQPEAGPGEPDQLTASLQPEPPAPARPSAGGPRARDVAQYLLSDSLFVWVLVNT
ACCVLMLVAKLIQCIVFGPLRVSERQHLKDKFWNFIFYKFIFIFGVLNVQTVEEVVMWCLWFAGLVFLHLMVQLCKDRFEYLSFSPTTPM
SSHGRVLSLLVAMLLSCCGLAAVCSITGYTHGMHTLAFMAAESLLVTVRTAHVILRYVIHLWDLNHEGTWEGKGTYVYYTDFVMELTLLS
LDLMHHIHMLLFGNIWLSMASLVIFMQLRYLFHEVQRRIRRHKNYLRVVGNMEARFAVATPEELAVNNDDCAICWDSMQAARKLPCGHLF
HNSCLRSWLEQDTSCPTCRMSLNIADNNRVREEHQGENLDENLVPVAAAEGRPRLNQHNHFFHFDGSRIASWLPSFSVEVMHTTNILGIT
QASNSQLNAMRIAGSKLSPRLQYFGQSLSGGQDLTMDGLVDLTVGAQGHVLLLRSQPVLRVKAIMEFNPREVARNVFECNDQVVKGKEAG
EVRVCLHVQKSTRDRLREGQIQSVVTYDLALDSGRPHSRAVFNETKNSTRRQTQVLGLTQTCETLKLQLPNCIEDPVSPIVLRLNFSLVG
TPLSAFGNLRPVLAEDAQRLFTALFPFEKNCGNDNICQDDLSITFSFMSLDCLVVGGPREFNVTVTVRNDGEDSYRTQVTFFFPLDLSYR
KVSTLQNQRSQRSWRLACESASSTEVSGALKSTSCSINHPIFPENSEVTFNITFDVDSKASLGNKLLLKANVTSENNMPRTNKTEFQLEL
PVKYAVYMVVTSHGVSTKYLNFTASENTSRVMQHQYQVSNLGQRSLPISLVFLVPVRLNQTVIWDRPQVTFSENLSSTCHTKERLPSHSD
FLAELRKAPVVNCSIAVCQRIQCDIPFFGIQEEFNATLKGNLSFDWYIKTSHNHLLIVSTAEILFNDSVFTLLPGQGAFVRSQTETKVEP

--------------------------------------------------------------

>4216_4216_2_AMFR-ITGAM_AMFR_chr16_56419830_ENST00000290649_ITGAM_chr16_31332561_ENST00000544665_length(amino acids)=1043AA_BP=460
MPLLFLERFPWPSLRTYTGLSGLALLGTIISAYRALSQPEAGPGEPDQLTASLQPEPPAPARPSAGGPRARDVAQYLLSDSLFVWVLVNT
ACCVLMLVAKLIQCIVFGPLRVSERQHLKDKFWNFIFYKFIFIFGVLNVQTVEEVVMWCLWFAGLVFLHLMVQLCKDRFEYLSFSPTTPM
SSHGRVLSLLVAMLLSCCGLAAVCSITGYTHGMHTLAFMAAESLLVTVRTAHVILRYVIHLWDLNHEGTWEGKGTYVYYTDFVMELTLLS
LDLMHHIHMLLFGNIWLSMASLVIFMQLRYLFHEVQRRIRRHKNYLRVVGNMEARFAVATPEELAVNNDDCAICWDSMQAARKLPCGHLF
HNSCLRSWLEQDTSCPTCRMSLNIADNNRVREEHQGENLDENLVPVAAAEGRPRLNQHNHFFHFDGSRIASWLPSFSVEVMHTTNILGIT
QASNSQLNAMRIAGSKLSPRLQYFGQSLSGGQDLTMDGLVDLTVGAQGHVLLLRSQPVLRVKAIMEFNPREVARNVFECNDQVVKGKEAG
EVRVCLHVQKSTRDRLREGQIQSVVTYDLALDSGRPHSRAVFNETKNSTRRQTQVLGLTQTCETLKLQLPNCIEDPVSPIVLRLNFSLVG
TPLSAFGNLRPVLAEDAQRLFTALFPFEKNCGNDNICQDDLSITFSFMSLDCLVVGGPREFNVTVTVRNDGEDSYRTQVTFFFPLDLSYR
KVSTLQNQRSQRSWRLACESASSTEVSGALKSTSCSINHPIFPENSEVTFNITFDVDSKASLGNKLLLKANVTSENNMPRTNKTEFQLEL
PVKYAVYMVVTSHGVSTKYLNFTASENTSRVMQHQYQVSNLGQRSLPISLVFLVPVRLNQTVIWDRPQVTFSENLSSTCHTKERLPSHSD
FLAELRKAPVVNCSIAVCQRIQCDIPFFGIQEEFNATLKGNLSFDWYIKTSHNHLLIVSTAEILFNDSVFTLLPGQGAFVRSQTETKVEP

--------------------------------------------------------------

>4216_4216_3_AMFR-ITGAM_AMFR_chr16_56419831_ENST00000290649_ITGAM_chr16_31332562_ENST00000287497_length(amino acids)=1043AA_BP=460
MPLLFLERFPWPSLRTYTGLSGLALLGTIISAYRALSQPEAGPGEPDQLTASLQPEPPAPARPSAGGPRARDVAQYLLSDSLFVWVLVNT
ACCVLMLVAKLIQCIVFGPLRVSERQHLKDKFWNFIFYKFIFIFGVLNVQTVEEVVMWCLWFAGLVFLHLMVQLCKDRFEYLSFSPTTPM
SSHGRVLSLLVAMLLSCCGLAAVCSITGYTHGMHTLAFMAAESLLVTVRTAHVILRYVIHLWDLNHEGTWEGKGTYVYYTDFVMELTLLS
LDLMHHIHMLLFGNIWLSMASLVIFMQLRYLFHEVQRRIRRHKNYLRVVGNMEARFAVATPEELAVNNDDCAICWDSMQAARKLPCGHLF
HNSCLRSWLEQDTSCPTCRMSLNIADNNRVREEHQGENLDENLVPVAAAEGRPRLNQHNHFFHFDGSRIASWLPSFSVEVMHTTNILGIT
QASNSQLNAMRIAGSKLSPRLQYFGQSLSGGQDLTMDGLVDLTVGAQGHVLLLRSQPVLRVKAIMEFNPREVARNVFECNDQVVKGKEAG
EVRVCLHVQKSTRDRLREGQIQSVVTYDLALDSGRPHSRAVFNETKNSTRRQTQVLGLTQTCETLKLQLPNCIEDPVSPIVLRLNFSLVG
TPLSAFGNLRPVLAEDAQRLFTALFPFEKNCGNDNICQDDLSITFSFMSLDCLVVGGPREFNVTVTVRNDGEDSYRTQVTFFFPLDLSYR
KVSTLQNQRSQRSWRLACESASSTEVSGALKSTSCSINHPIFPENSEVTFNITFDVDSKASLGNKLLLKANVTSENNMPRTNKTEFQLEL
PVKYAVYMVVTSHGVSTKYLNFTASENTSRVMQHQYQVSNLGQRSLPISLVFLVPVRLNQTVIWDRPQVTFSENLSSTCHTKERLPSHSD
FLAELRKAPVVNCSIAVCQRIQCDIPFFGIQEEFNATLKGNLSFDWYIKTSHNHLLIVSTAEILFNDSVFTLLPGQGAFVRSQTETKVEP

--------------------------------------------------------------

>4216_4216_4_AMFR-ITGAM_AMFR_chr16_56419831_ENST00000290649_ITGAM_chr16_31332562_ENST00000544665_length(amino acids)=1043AA_BP=460
MPLLFLERFPWPSLRTYTGLSGLALLGTIISAYRALSQPEAGPGEPDQLTASLQPEPPAPARPSAGGPRARDVAQYLLSDSLFVWVLVNT
ACCVLMLVAKLIQCIVFGPLRVSERQHLKDKFWNFIFYKFIFIFGVLNVQTVEEVVMWCLWFAGLVFLHLMVQLCKDRFEYLSFSPTTPM
SSHGRVLSLLVAMLLSCCGLAAVCSITGYTHGMHTLAFMAAESLLVTVRTAHVILRYVIHLWDLNHEGTWEGKGTYVYYTDFVMELTLLS
LDLMHHIHMLLFGNIWLSMASLVIFMQLRYLFHEVQRRIRRHKNYLRVVGNMEARFAVATPEELAVNNDDCAICWDSMQAARKLPCGHLF
HNSCLRSWLEQDTSCPTCRMSLNIADNNRVREEHQGENLDENLVPVAAAEGRPRLNQHNHFFHFDGSRIASWLPSFSVEVMHTTNILGIT
QASNSQLNAMRIAGSKLSPRLQYFGQSLSGGQDLTMDGLVDLTVGAQGHVLLLRSQPVLRVKAIMEFNPREVARNVFECNDQVVKGKEAG
EVRVCLHVQKSTRDRLREGQIQSVVTYDLALDSGRPHSRAVFNETKNSTRRQTQVLGLTQTCETLKLQLPNCIEDPVSPIVLRLNFSLVG
TPLSAFGNLRPVLAEDAQRLFTALFPFEKNCGNDNICQDDLSITFSFMSLDCLVVGGPREFNVTVTVRNDGEDSYRTQVTFFFPLDLSYR
KVSTLQNQRSQRSWRLACESASSTEVSGALKSTSCSINHPIFPENSEVTFNITFDVDSKASLGNKLLLKANVTSENNMPRTNKTEFQLEL
PVKYAVYMVVTSHGVSTKYLNFTASENTSRVMQHQYQVSNLGQRSLPISLVFLVPVRLNQTVIWDRPQVTFSENLSSTCHTKERLPSHSD
FLAELRKAPVVNCSIAVCQRIQCDIPFFGIQEEFNATLKGNLSFDWYIKTSHNHLLIVSTAEILFNDSVFTLLPGQGAFVRSQTETKVEP

--------------------------------------------------------------

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Fusion Protein Functional Features


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr16:56419831/chr16:31332562)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
AMFR

Q9UKV5

ITGAM

P11215

FUNCTION: E3 ubiquitin-protein ligase that mediates the polyubiquitination of lysine and cysteine residues on target proteins, such as CD3D, CYP3A4, CFTR, INSIG1, SOAT2/ACAT2 and APOB for proteasomal degradation (PubMed:10456327, PubMed:11724934, PubMed:12670940, PubMed:19103148, PubMed:24424410, PubMed:28604676). Component of a VCP/p97-AMFR/gp78 complex that participates in the final step of endoplasmic reticulum-associated degradation (ERAD) (PubMed:10456327, PubMed:11724934, PubMed:19103148, PubMed:24424410). The VCP/p97-AMFR/gp78 complex is involved in the sterol-accelerated ERAD degradation of HMGCR through binding to the HMGCR-INSIG1 complex at the ER membrane (PubMed:16168377, PubMed:22143767). In addition, interaction of AMFR with AUP1 facilitates interaction of AMFR with ubiquitin-conjugating enzyme UBE2G2 and ubiquitin ligase RNF139, leading to sterol-induced HMGCR ubiquitination (PubMed:23223569). The ubiquitinated HMGCR is then released from the ER into the cytosol for subsequent destruction (PubMed:16168377, PubMed:22143767, PubMed:23223569). In addition to ubiquitination on lysine residues, catalyzes ubiquitination on cysteine residues: together with INSIG1, mediates polyubiquitination of SOAT2/ACAT2 at 'Cys-277', leading to its degradation when the lipid levels are low (PubMed:28604676). Catalyzes ubiquitination and subsequent degradation of INSIG1 when cells are depleted of sterols (PubMed:17043353). Mediates polyubiquitination of INSIG2 at 'Cys-215' in some tissues, leading to its degradation (PubMed:31953408). Also regulates ERAD through the ubiquitination of UBL4A a component of the BAG6/BAT3 complex (PubMed:21636303). Also acts as a scaffold protein to assemble a complex that couples ubiquitination, retranslocation and deglycosylation (PubMed:21636303). Mediates tumor invasion and metastasis as a receptor for the GPI/autocrine motility factor (PubMed:10456327). In association with LMBR1L and UBAC2, negatively regulates the canonical Wnt signaling pathway in the lymphocytes by promoting the ubiquitin-mediated degradation of CTNNB1 and Wnt receptors FZD6 and LRP6 (PubMed:31073040). {ECO:0000269|PubMed:10456327, ECO:0000269|PubMed:11724934, ECO:0000269|PubMed:12670940, ECO:0000269|PubMed:16168377, ECO:0000269|PubMed:17043353, ECO:0000269|PubMed:19103148, ECO:0000269|PubMed:21636303, ECO:0000269|PubMed:22143767, ECO:0000269|PubMed:23223569, ECO:0000269|PubMed:24424410, ECO:0000269|PubMed:28604676, ECO:0000269|PubMed:31073040, ECO:0000269|PubMed:31953408}.FUNCTION: Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PubMed:9558116, PubMed:20008295). It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration (PubMed:28807980). In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (PubMed:21193407). May regulate phagocytosis-induced apoptosis in extravasated neutrophils (By similarity). May play a role in mast cell development (By similarity). Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development (By similarity). {ECO:0000250|UniProtKB:P05555, ECO:0000269|PubMed:20008295, ECO:0000269|PubMed:21193407, ECO:0000269|PubMed:28807980, ECO:0000269|PubMed:9558116, ECO:0000305}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page

* Minus value of BPloci means that the break pointn is located before the CDS.
- Retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneAMFRchr16:56419830chr16:31332561ENST00000290649-1014122_142460.0644.0TransmembraneHelical
HgeneAMFRchr16:56419830chr16:31332561ENST00000290649-1014145_165460.0644.0TransmembraneHelical
HgeneAMFRchr16:56419830chr16:31332561ENST00000290649-1014186_206460.0644.0TransmembraneHelical
HgeneAMFRchr16:56419830chr16:31332561ENST00000290649-1014215_235460.0644.0TransmembraneHelical
HgeneAMFRchr16:56419830chr16:31332561ENST00000290649-1014276_296460.0644.0TransmembraneHelical
HgeneAMFRchr16:56419830chr16:31332561ENST00000290649-1014429_449460.0644.0TransmembraneHelical
HgeneAMFRchr16:56419830chr16:31332561ENST00000290649-101482_102460.0644.0TransmembraneHelical
HgeneAMFRchr16:56419831chr16:31332562ENST00000290649-1014122_142460.0644.0TransmembraneHelical
HgeneAMFRchr16:56419831chr16:31332562ENST00000290649-1014145_165460.0644.0TransmembraneHelical
HgeneAMFRchr16:56419831chr16:31332562ENST00000290649-1014186_206460.0644.0TransmembraneHelical
HgeneAMFRchr16:56419831chr16:31332562ENST00000290649-1014215_235460.0644.0TransmembraneHelical
HgeneAMFRchr16:56419831chr16:31332562ENST00000290649-1014276_296460.0644.0TransmembraneHelical
HgeneAMFRchr16:56419831chr16:31332562ENST00000290649-1014429_449460.0644.0TransmembraneHelical
HgeneAMFRchr16:56419831chr16:31332562ENST00000290649-101482_102460.0644.0TransmembraneHelical
HgeneAMFRchr16:56419830chr16:31332561ENST00000290649-1014341_379460.0644.0Zinc fingerRING-type
HgeneAMFRchr16:56419831chr16:31332562ENST00000290649-1014341_379460.0644.0Zinc fingerRING-type
TgeneITGAMchr16:56419830chr16:31332561ENST000002874971330592_600569.01153.0Calcium bindingOntology_term=ECO:0000255
TgeneITGAMchr16:56419830chr16:31332561ENST000005446651330592_600570.01154.0Calcium bindingOntology_term=ECO:0000255
TgeneITGAMchr16:56419831chr16:31332562ENST000002874971330592_600569.01153.0Calcium bindingOntology_term=ECO:0000255
TgeneITGAMchr16:56419831chr16:31332562ENST000005446651330592_600570.01154.0Calcium bindingOntology_term=ECO:0000255
TgeneITGAMchr16:56419830chr16:31332561ENST0000028749713301131_1135569.01153.0MotifNote=GFFKR motif
TgeneITGAMchr16:56419830chr16:31332561ENST0000054466513301131_1135570.01154.0MotifNote=GFFKR motif
TgeneITGAMchr16:56419831chr16:31332562ENST0000028749713301131_1135569.01153.0MotifNote=GFFKR motif
TgeneITGAMchr16:56419831chr16:31332562ENST0000054466513301131_1135570.01154.0MotifNote=GFFKR motif
TgeneITGAMchr16:56419830chr16:31332561ENST000002874971330569_629569.01153.0RepeatFG-GAP 7
TgeneITGAMchr16:56419830chr16:31332561ENST000005446651330569_629570.01154.0RepeatFG-GAP 7
TgeneITGAMchr16:56419831chr16:31332562ENST000002874971330569_629569.01153.0RepeatFG-GAP 7
TgeneITGAMchr16:56419831chr16:31332562ENST000005446651330569_629570.01154.0RepeatFG-GAP 7
TgeneITGAMchr16:56419830chr16:31332561ENST0000028749713301129_1152569.01153.0Topological domainCytoplasmic
TgeneITGAMchr16:56419830chr16:31332561ENST0000054466513301129_1152570.01154.0Topological domainCytoplasmic
TgeneITGAMchr16:56419831chr16:31332562ENST0000028749713301129_1152569.01153.0Topological domainCytoplasmic
TgeneITGAMchr16:56419831chr16:31332562ENST0000054466513301129_1152570.01154.0Topological domainCytoplasmic
TgeneITGAMchr16:56419830chr16:31332561ENST0000028749713301105_1128569.01153.0TransmembraneHelical
TgeneITGAMchr16:56419830chr16:31332561ENST0000054466513301105_1128570.01154.0TransmembraneHelical
TgeneITGAMchr16:56419831chr16:31332562ENST0000028749713301105_1128569.01153.0TransmembraneHelical
TgeneITGAMchr16:56419831chr16:31332562ENST0000054466513301105_1128570.01154.0TransmembraneHelical

- Not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneAMFRchr16:56419830chr16:31332561ENST00000290649-1014456_498460.0644.0DomainCUE
HgeneAMFRchr16:56419831chr16:31332562ENST00000290649-1014456_498460.0644.0DomainCUE
HgeneAMFRchr16:56419830chr16:31332561ENST00000290649-1014622_640460.0644.0RegionVCP/p97-interacting motif (VIM)
HgeneAMFRchr16:56419831chr16:31332562ENST00000290649-1014622_640460.0644.0RegionVCP/p97-interacting motif (VIM)
TgeneITGAMchr16:56419830chr16:31332561ENST000002874971330465_473569.01153.0Calcium bindingOntology_term=ECO:0000255
TgeneITGAMchr16:56419830chr16:31332561ENST000002874971330529_537569.01153.0Calcium bindingOntology_term=ECO:0000255
TgeneITGAMchr16:56419830chr16:31332561ENST000005446651330465_473570.01154.0Calcium bindingOntology_term=ECO:0000255
TgeneITGAMchr16:56419830chr16:31332561ENST000005446651330529_537570.01154.0Calcium bindingOntology_term=ECO:0000255
TgeneITGAMchr16:56419831chr16:31332562ENST000002874971330465_473569.01153.0Calcium bindingOntology_term=ECO:0000255
TgeneITGAMchr16:56419831chr16:31332562ENST000002874971330529_537569.01153.0Calcium bindingOntology_term=ECO:0000255
TgeneITGAMchr16:56419831chr16:31332562ENST000005446651330465_473570.01154.0Calcium bindingOntology_term=ECO:0000255
TgeneITGAMchr16:56419831chr16:31332562ENST000005446651330529_537570.01154.0Calcium bindingOntology_term=ECO:0000255
TgeneITGAMchr16:56419830chr16:31332561ENST000002874971330150_328569.01153.0DomainVWFA
TgeneITGAMchr16:56419830chr16:31332561ENST000005446651330150_328570.01154.0DomainVWFA
TgeneITGAMchr16:56419831chr16:31332562ENST000002874971330150_328569.01153.0DomainVWFA
TgeneITGAMchr16:56419831chr16:31332562ENST000005446651330150_328570.01154.0DomainVWFA
TgeneITGAMchr16:56419830chr16:31332561ENST00000287497133018_75569.01153.0RepeatFG-GAP 1
TgeneITGAMchr16:56419830chr16:31332561ENST000002874971330339_390569.01153.0RepeatFG-GAP 3
TgeneITGAMchr16:56419830chr16:31332561ENST000002874971330391_442569.01153.0RepeatFG-GAP 4
TgeneITGAMchr16:56419830chr16:31332561ENST000002874971330443_503569.01153.0RepeatFG-GAP 5
TgeneITGAMchr16:56419830chr16:31332561ENST000002874971330506_564569.01153.0RepeatFG-GAP 6
TgeneITGAMchr16:56419830chr16:31332561ENST00000287497133076_135569.01153.0RepeatFG-GAP 2
TgeneITGAMchr16:56419830chr16:31332561ENST00000544665133018_75570.01154.0RepeatFG-GAP 1
TgeneITGAMchr16:56419830chr16:31332561ENST000005446651330339_390570.01154.0RepeatFG-GAP 3
TgeneITGAMchr16:56419830chr16:31332561ENST000005446651330391_442570.01154.0RepeatFG-GAP 4
TgeneITGAMchr16:56419830chr16:31332561ENST000005446651330443_503570.01154.0RepeatFG-GAP 5
TgeneITGAMchr16:56419830chr16:31332561ENST000005446651330506_564570.01154.0RepeatFG-GAP 6
TgeneITGAMchr16:56419830chr16:31332561ENST00000544665133076_135570.01154.0RepeatFG-GAP 2
TgeneITGAMchr16:56419831chr16:31332562ENST00000287497133018_75569.01153.0RepeatFG-GAP 1
TgeneITGAMchr16:56419831chr16:31332562ENST000002874971330339_390569.01153.0RepeatFG-GAP 3
TgeneITGAMchr16:56419831chr16:31332562ENST000002874971330391_442569.01153.0RepeatFG-GAP 4
TgeneITGAMchr16:56419831chr16:31332562ENST000002874971330443_503569.01153.0RepeatFG-GAP 5
TgeneITGAMchr16:56419831chr16:31332562ENST000002874971330506_564569.01153.0RepeatFG-GAP 6
TgeneITGAMchr16:56419831chr16:31332562ENST00000287497133076_135569.01153.0RepeatFG-GAP 2
TgeneITGAMchr16:56419831chr16:31332562ENST00000544665133018_75570.01154.0RepeatFG-GAP 1
TgeneITGAMchr16:56419831chr16:31332562ENST000005446651330339_390570.01154.0RepeatFG-GAP 3
TgeneITGAMchr16:56419831chr16:31332562ENST000005446651330391_442570.01154.0RepeatFG-GAP 4
TgeneITGAMchr16:56419831chr16:31332562ENST000005446651330443_503570.01154.0RepeatFG-GAP 5
TgeneITGAMchr16:56419831chr16:31332562ENST000005446651330506_564570.01154.0RepeatFG-GAP 6
TgeneITGAMchr16:56419831chr16:31332562ENST00000544665133076_135570.01154.0RepeatFG-GAP 2
TgeneITGAMchr16:56419830chr16:31332561ENST00000287497133017_1104569.01153.0Topological domainExtracellular
TgeneITGAMchr16:56419830chr16:31332561ENST00000544665133017_1104570.01154.0Topological domainExtracellular
TgeneITGAMchr16:56419831chr16:31332562ENST00000287497133017_1104569.01153.0Topological domainExtracellular
TgeneITGAMchr16:56419831chr16:31332562ENST00000544665133017_1104570.01154.0Topological domainExtracellular


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Fusion Protein Structures

check button PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.
Fusion protein PDB link (fusion AA seq ID in FusionPDB)HgeneHchrHbpHstrandTgeneTchrTbpTstrandAA seqLen(AA seq)
PDB file >>>1821_AMFR_56419830_ITGAM_31332561_ranked_0.pdbAMFR5641983056419830ENST00000544665ITGAMchr1631332561+
MPLLFLERFPWPSLRTYTGLSGLALLGTIISAYRALSQPEAGPGEPDQLTASLQPEPPAPARPSAGGPRARDVAQYLLSDSLFVWVLVNT
ACCVLMLVAKLIQCIVFGPLRVSERQHLKDKFWNFIFYKFIFIFGVLNVQTVEEVVMWCLWFAGLVFLHLMVQLCKDRFEYLSFSPTTPM
SSHGRVLSLLVAMLLSCCGLAAVCSITGYTHGMHTLAFMAAESLLVTVRTAHVILRYVIHLWDLNHEGTWEGKGTYVYYTDFVMELTLLS
LDLMHHIHMLLFGNIWLSMASLVIFMQLRYLFHEVQRRIRRHKNYLRVVGNMEARFAVATPEELAVNNDDCAICWDSMQAARKLPCGHLF
HNSCLRSWLEQDTSCPTCRMSLNIADNNRVREEHQGENLDENLVPVAAAEGRPRLNQHNHFFHFDGSRIASWLPSFSVEVMHTTNILGIT
QASNSQLNAMRIAGSKLSPRLQYFGQSLSGGQDLTMDGLVDLTVGAQGHVLLLRSQPVLRVKAIMEFNPREVARNVFECNDQVVKGKEAG
EVRVCLHVQKSTRDRLREGQIQSVVTYDLALDSGRPHSRAVFNETKNSTRRQTQVLGLTQTCETLKLQLPNCIEDPVSPIVLRLNFSLVG
TPLSAFGNLRPVLAEDAQRLFTALFPFEKNCGNDNICQDDLSITFSFMSLDCLVVGGPREFNVTVTVRNDGEDSYRTQVTFFFPLDLSYR
KVSTLQNQRSQRSWRLACESASSTEVSGALKSTSCSINHPIFPENSEVTFNITFDVDSKASLGNKLLLKANVTSENNMPRTNKTEFQLEL
PVKYAVYMVVTSHGVSTKYLNFTASENTSRVMQHQYQVSNLGQRSLPISLVFLVPVRLNQTVIWDRPQVTFSENLSSTCHTKERLPSHSD
FLAELRKAPVVNCSIAVCQRIQCDIPFFGIQEEFNATLKGNLSFDWYIKTSHNHLLIVSTAEILFNDSVFTLLPGQGAFVRSQTETKVEP
1043


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pLDDT score distribution

check button pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.
AMFR_pLDDT.png
all structure
all structure
ITGAM_pLDDT.png
all structure
all structure

check button pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.
all structure


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Ramachandran Plot of Fusion Protein Structure


check button Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.
Fusion AA seq ID in FusionPDB and their Ramachandran plots

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Fusion Protein-Protein Interaction


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)
GenePPI interactors


check button Protein-protein interactors based on sequence similarity (STRING)
GeneSTRING network
AMFR
ITGAM


check button - Retained interactions in fusion protein (protein functional feature from UniProt).
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost interactions due to fusion (protein functional feature from UniProt).
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs to AMFR-ITGAM


check button Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDrugSourcePMID

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Related Diseases to AMFR-ITGAM


check button Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDiseaseSourcePMID

check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource